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Shandon Excelsior ES®

1. OR move down the list to a blank position Go to empty position Key becomes available aaa 3 A new key is displayed CHANGE NAME Select this to display a OWERTY keyboard on the screen as shown 42 Shandon Excelsior ES Operator Guide 4 Highlight the first letter move around the displayed OWERTY keys with the keypad as shown The procedure is easy using both hands Select CAPS LOCK to display capital letters Select CONTROL to display accents umlauts and other characters 5 Press to put the highlighted character into the Reagent Name Select CAPS LOCK to return to plain letters 6 Repeat for all characters required 7 Press LEX J to set the Reagent Name and return to the previous menu Move down to the next item in the list Shandon Excelsior ES Operator Guide 43 3 Load Reagent Groups Loading Wax Use this menu to load an instrument with reagents Excelsior ES uses reagent bottles between 3 8 L and 5 0 L The Load Reagents screen below shows all bottles and wax containers empty You have to e Put the reagents in the correct cabinet positions and insert the colour coded reagent tubes with evaporation cap e Confirm in the screen that they have been loaded Highlight reagent Fill Container Confirm Loaded e Move down the reagent list and load reagents in the order wax flush reagents dehydrants clearants Fixatives are loaded from the Quality
2. CAN CSA C22 2 No 1010 1 92 Inc A2 1997 UL Std No 61010A 1 Issued by K Waldron Ouality Manager Thermo Fisher Scientific Anatomical Pathology Diagnostics Division Wise OIA Date 27 April 2007 Optional accessories considered subject to the In Vitro Diagnostics Directive IVDD are specifically identified on this Declaration of Conformity Further supplies of standard accessories are treated as spares Convenience aids offered as accessories are not subject to the IVDD Shandon Excelsior ES Operator Guide Introduction WARNING Reagent List Fixatives Dehydrants Clearants Infiltrants Flush Reagents Appendix B Approved Reagents This Section lists all the reagents specified by Thermo for use with the Shandon Excelsior ES tissue processor If you want to use a reagent not included in this list contact your Thermo agent for advice Do not use any reagents containing calcium chloride A REFER TO THE MATERIAL SAFETY DATA SHEETS MSDs WHEN HANDLING THE REAGENTS LISTED IN APPENDIX C A Formalin Glyo Fixx Ethanol Methanol up to 10 in ethanol IMS Industrial Methylated Spirits Isopropyl alcohol Xylene Toluene Shandon Xylene Substitute Chloroform Paraffin Wax Xylene Toluene Shandon Xylene Substitute Shandon Excelsior ES Operator Guide 119 Alcohols from dehydrants list Water Cleaning Agents Dilute detergent Water 120 Shandon Excelsior ES Operator Guide Introduc
3. contained in the Shandon Excelsior ES Service Manual A78410101 Any problems and queries should be referred to your supplier Shandon Excelsior ES Operator Guide Disposal of Sealed Lea Acid Batteries The Sealed Lead Acid batteries within Excelsior ES need to be replaced every two years Note If Excelsior ES has been operated in very low temperatures for much of the time or has been exposed to frequent mains failures the batteries should be replaced every year Batteries must only be replaced in pairs The battery manufacturers Yuasa Battery Sale UK Ltd advise their customers to comply with the relevant regulations within their particular country regarding disposal of this type of battery The batteries used in Excelsior ES are NP 12 12 12V 12Ah valve regulated sealed lead acid type rechargeable battery These batteries are classified as Class 8 Group III UN No 2800 Batteries wet non spillable electric storage special provision A67 and meet all requirements of the International Air transport Association L A T A Dangerous Goods Regulations Shandon Excelsior ES Operator Guide 11 12 Shandon Excelsior ES Operator Guide Chapter 1 Introducing Excelsior ES Overview The Shandon Excelsior ES is an enclosed automated tissue processor combining custom programming with simple operation and reagent management Screen Reaction Chamber Keypad Reagents Shandon Excelsior ES Op
4. to restore the original factory programs This eliminates any user defined programs and changes to the default programs 54 Shandon Excelsior ES Operator Guide 5 Customisation Hospital and Laboratory Name Shift Start and End Cassette Counting Level Key Available in Daily Operations The customisation screen is shown below and use of the setup options described Select HOSPITAL NAME to display the OWERTY keyboard Follow the text entry procedure described on page 43 and press when the name is complete Repeat for LABORATORY NAME The text is now displayed at the top of the screen Considering that Excelsior ES should be powered ON at all times entering details here switches the screensaver ON displaying a blank screen at times when Excelsior ES is left unattended Select ON to enable the cassette counting feature Excelsior ES prompts you to enter numbers as you add cassettes to the reaction chamber the instrument does NOT count the cassettes In Daily Operations mode a LEVEL option key is displayed when the reaction chamber lid is opened Shandon Excelsior ES Operator Guide 55 Select OFF to remove the LEVEL option from the Daily Operations mode For all processing the reaction chamber is filled to the random basket level The LEVEL key is ALWAYS enabled in Menu Mode Restart level If you select ON whenever the reaction chamber lid is opened during processing Excelsior ES
5. Do not modify the instrument if unauthorised modifications are carried out the instrument may be made unsafe and the warranty may be invalidated Potentially lethal voltages above 110V AC or 50V DC are present inside the instrument The instrument must be properly connected to a good earth Ground via the mains input supply Do not remove any panels or covers There are no user serviceable parts inside the instrument It is important to maintain normal standards of safety and Good Laboratory Practices Always use common sense and the best known practice when operating the instrument Shandon Excelsior ES Operator Guide Excelsior ES as supplied conforms with the international safety standards IEC 61010 2 010 However adding chemical reagents introduces potential hazards Good Laboratory Practice must be followed when dealing with these chemicals and operators must consider the potential for hazard when dealing with particular chemicals BEFORE using reagents ALWAYS refer to your own laboratory procedures and manufacturer safety data sheets MSDS Many of the reagents used with Excelsior are flammable Do not introduce any source of ignition into or near the instrument once it has been loaded with reagents Sources of ignition include electrostatic discharge resulting from a build up of static electricity This can under certain conditions result in ignition of fumes from any reagent used with Excelsior ES Good Lab
6. ES Operator Guide 23 Loading Specimens 1 Open the reaction chamber push the knob and lift the lid to display the red status screen Reaction Chamber Status Available Add specimens Use LEVEL key to select appropriate level to cover specimen baskets Close the lid i Instructions Press START to begin the process shown below or ss PROCESS SELECT Process 2 Biopsies Process End Time pag Tuesday 09 20 Processing end time ESSA fiji In 098 d GS un mu Process Select Select Change End Time Current pro gram Select to change Select to start pro Select to change baskets displayed cessing end time and fill level 2 Load baskets with a basket lid into the chamber stacked in uniform pairs and aligned so they sit properly on the agitation drive pins 3 Close the reaction chamber lid pushing down on both sides of the knob to ensure a good seal If there is no LEVEL key shown go to Starting Current Process Level Key Select LEVEL once for each pair of organised baskets up to three times for three pairs of baskets Select LEVEL four times for a random basket Ensure that the screen shows the baskets you put in the chamber If too many baskets are shown press the LEVEL key until the display is correct If you do not select LEVEL Excelsior ES assumes a random basket and fills the chamber to the highest level 24 Shandon Excelsior ES Operator Guide Starting the Current
7. Guide 21 Removing 1 Select DRAIN ALL CHAMBER and wait till draining Specimens stops 2 Open the lid and remove basket s If baskets are still shown in the chamber press the LEVEL key to clear them 3 Wipe out the reaction chamber With the LEVEL key enabled you can remove baskets a level at a time after infiltration 1 Select DRAIN NEXT LEVEL and wait till draining stops 2 Open the lid and remove basket s 3 Press LEVEL so the screen shows the number of baskets in the chamber Repeat steps 1 3 until the chamber is empty 4 Wipe out the reaction chamber Flushing and When the screen shows there are no more baskets in the chamber Rinsing the he Flush screen appears automatically you are required to flush PP Yy SY g Reaction Chamber the wax in the reaction chamber before starting the next process 1 Put empty basket s back in the reaction chamber and close the lid 2 From the displayed Flush screen select START For a different Flush program select SELECT FLUSH 3 When the monitoring screen shows the Flush program has finished remove basket s 4 Select OK Excelsior ES is ready to start a new process run 28 Shandon Excelsior ES Operator Guide About the Ouality Control Screen Setting Usage Limits Press QUALITY CONTROL to display the Quality Control screen below It shows e Count number of times reagents and filters have been used e Limit the numbe
8. are shown press the LEVEL key until the display is correct If you do not select LEVEL Excelsior ES assumes a random basket and fills the chamber to the highest level 64 Shandon Excelsior ES Operator Guide Select IMMEDIATE START or DELAYED START according to the current program for daytime or overnight processing Monitoring the The monitoring screen is displayed shown below Process Start Reagent Processing Current Step Details The status is shown as First Fill Wait Ensure that e The first reagent is drawn into the reaction chamber and the status line changes e The monitoring screen shows that the chamber is filled to the correct level e The baskets are completely covered with reagent Shandon Excelsior ES Operator Guide 65 Starting a Different Process 66 Changing the End Time Changing the End Day I 219 T Select PROCESS SELECT to display the Process Select screen Highlight the program you want and check that the End Time is correct Select IMMEDIATE START or DELAYED START 9 19 2005 lt Current Day Time Duration of highlighted programme Start End Day Time Select CHANGE END TIME Select SET END TIME Enter the time with the number keys and use the AM PM key if necessary Ensure that End Time and Process are correct Select DELAYED START Select CHANGE END TIME to highlight the time Select SET END TIME Sele
9. assumes that tissues have been added for processing and fills the chamber to the random basket level The table below summarises the fill level in the chamber after a restart Level ON Level OFF Random Random On Hold Alarm The number of minutes a process can be left on hold before an alarm is sounded Enter a number between 1 and 30 minutes Process Start When you open the lid the currently available program is always Selection Options displayed Process Start selection options below let you choose different programs for daytime and overnight processing Daytime Program The program used during the day Select from the program list displayed Available Until The latest time Excelsior ES will offer the daytime program Use the number keys to enter the time for Excelsior ES to stop displaying daytime programs NONE SELECTED You can choose not to have a daytime program appear when the lid is opened 56 Shandon Excelsior ES Operator Guide Overnight Program Preferred End Time Working Week 6 Reagent Management The program to be used overnight Select from the program list displayed The preferred time for the overnight program to end Enter the reguired time with the number key Note NONE SELECTED cannot be chosen for both Daytime and Overnight programs A Whether the working week is 5 6 or 7 days Consider the working week to start on a Monday Select the option required When all the in
10. is important that the certificate is forwarded by post or fax and a copy attached to the exterior of the container Containers will not be opened until the company is in possession of the required certificate This form MUST be completed by the customer and NOT by a Thermo Fisher or distributor employee If an instrument or part is to be returned to Thermo Fisher Scientific please note the following 1 Ifthe instrument or any part of it has been exposed to or been in contact with potential pathogenic or radioactive material itis essential that it is decontaminated 2 Set procedures are laid down in the European Health and Safety Directives for decontamination To avoid any misunderstanding we request that all instruments or parts returned to us must be accompanied by a certificate stating the following We certify that this Model Serial No has not been exposed to pathogenic radioactive or other hazardous material and has been cleaned OR has been decontaminated and cleaned if exposed to the above according to approved procedures following exposure to Has the instrument been used for work with human or animal Transmissible Spongiform Encephalopathies e g Creutzfeld Jacob disease Scrapie or BSE YES NO If yes please contact Thermo Fisher Service before taking any further action Position Name Block Capitals Company or Organisation Full Address Part 2 Guidelines for Returning Instruments Please use the c
11. language and select SELECT The screen displays the battery isolation switch and heater reset alert icons The icons must be cleared before any further operations 1 Turn ON the battery isolation switch Shandon Excelsior ES Operator Guide 91 2 Press the heater reset switch The icons and switches are shown the inset shows the view inside the cabinet behind the right door above the flush reagent bottles Leave the battery switched on and do not switch it off unless instructed to do so Note 1 When the Heater reset switch is set and the Battery Isolation Switch is turned on the icons will disappear from the display First battery charging after switching on both the main I O power switch and the Battery isolation Switch the instrument should be left for a period of 14 hours overnight to ensure the battery is fully charged Standby For continuous and consistent processing The instrument should be powered ON at all times Press the Standby key d5 to turn off the screen and lock the keyboard processing continues E qr 13 333 1 1 2 3 4 5 e 7 8 9 0 Note 92 Shandon Excelsior ES Operator Guide ls The red LED on the Standby button flashes when the instrument is in STANDBY mode To unlock the keyboard and turn the screen on press the C5 button again Powering OFF for Ifthe instrument is to be left unattended for long periods of extended time time or is to be moved carry
12. nr CE RETE Demo Mode Remote communications Access code unlocked Alarm sounded Door open Reagent Quality Control issue 94 Shandon Excelsior ES Operator Guide OI A Concept Demonstration program is currently active The pink screen background shows that the program is not live Press C on the keypad to exit the demonstration Reaction Chamber Heater Trip Processing cannot start Open the top right cabinet door and press the Reset switch Access Codes have been unlocked The name of the person who has unlocked the codes is displayed underneath the icon To re enable Access codes press non the keypad Cabinet Doors Open This condition brings up the Ouality Control screen Close the lower doors then press on the keypad to close the Ouality Control screen Battery Isolation Switch is not on Open the top right cabinet door and turn the rocker switch ON to restore battery backup and ensure power to the instrument Mains Power failure the instrument is running on battery backup power available power displayed Inspect mains power connections power cable and mains supply Ouality Control Alert Open the Ouality Control screen for details Hardware Issue There is a problem with the instrument Processing cannot start in this condition Press on the keypad to display the Fault Status screen see below for details Remote Alarm A remote alarm has sounded The icon shows whether it is Alarm 1 or Alarm
13. of the screen only e For dehydrants clearants and infiltrants wax the Quality Control screen is displayed automatically at the beginning of a process run If alcohol quality at bottle A1 is set as the trigger for rotation a triangle is displayed above the alcohol quality gauge When you see the yellow warning triangle alert inside fixative and flush reagent bottles you should change the reagents before running the next process Open the lower doors the Quality Control screen appears Then 1 Remove the reagents and tell Excelsior ES a Take out the Fix1 and Fix2 bottles and discard them b Highlight Fix1 and select REMOVE c Highlight Fix2 and select REMOVE 30 Shandon Excelsior ES Operator Guide Replacing Flush Reagents F1 3 Reagent Rotation Overview 2 Place the new reagents and tell Excelsior ES a Put new bottles of fixative in positions Fix and Fix2 b Highlight Fix2 and select NEW c Highlight Fix1 and select NEW This zeroes the Quality Control counter and removes the warning triangles from the screen Open the lower doors and remove flush reagents in the order F2 F1 F3 In the Quality Control screen 1 Highlight F2 and select REMOVE 2 Repeat for F1 and F3 3 Place the new reagents in positions F3 F1 F2 In the Quality Control screen highlight each reagent in turn and select NEW Excelsior ES rotation is a unique in process system for maintaining dehydra
14. out the following steps L Ensure that any processing programs have finished Carry out a Flush procedure Press the O OFF side of the main I O power switch to switch off Excelsior ES Wait approximately 45 seconds Press the O OFF side of the Battery Isolation Switch to isolate the battery Shandon Excelsior ES Operator Guide 93 Chapter 6 Troubleshooting Introduction The instrument systems place the highest priority on specimen integrity and processing quality Excelsior ES continually monitors its status giving clear visual and audible warnings in the event of a problem and providing extensive specimen safety measures The Thermo logo at the top of the screen is replaced by alert icons a highly visible indicator of instrument issues The Quality Control and Fault Status screens enable quick diagnosis e The rechargeable battery provides backup power if there is a mains power failure Asounding alarm with remote facility can send warnings out of working hours See page 79 for details This chapter contains the following sections e The alert icons explained and procedures to clear them e TABLE 1 on page 98 lists apparent instrument malfunctions with possible causes and suggested remedies e TABLES 2 and 3 on page 98 list common processing problems possible causes and suggested actions TABLE 4 on page 102 answers Frequently Asked Questions Back up power switch OFF Hardware Fault
15. place above the two right hand wax baths Fit a waste liner into the waste wax tray and make sure that the top of the liner is folded over the top of the tray Fit the lid To remove the waste wax open the wax door and carefully slide the waste wax tray from the instrument THE WASTE WAX TRAY CONTAINS HOT MOLTEN WAX KEEP THE TRAY LEVEL AT ALL TIMES DO NOT REMOVE THE TRAY AND LINER UNTIL THE WAX HAS SET Place the tray in a cool well ventilated area away from the instrument and allow to set When the waste wax has set remove the waste bag from the waste wax tray and discard the solid wax in accordance with local procedures and regulations Keep the waste wax tray safely for reuse Ensure that there is always a waste wax tray in the instrument with a correctly Shandon Excelsior ES Operator Guide Cleaning Wax Baths Reaction Chamber fitted liner A spare waste wax tray is provided Slide the tray back into its position above the wax baths and close the access doors THE METAL PLATES IN THE BASE OF THE WAX BATHS ARE HOT ALWAYS WEAR GLOVES After the old wax is discarded Wax Bath 3 is empty a Open the left hand wax access door Use absorbent paper to wipe out any remaining wax from the wax bath Make sure that no paper is left in the wax bath Open the lid and use the plastic spatula provided to remove any solidified wax from the lid top and sides of the Reaction Chamber If necessary wipe the surfac
16. process to absolute alcohol and reprocess 1 Extend exposure time 2 Use vacuum 3 Bisect if encapsulated speci men or produce thinner block 4 Reinfiltrate Use vacuum to promote infiltration Shandon Excelsior ES Operator Guide 99 100 TABLE 3 PROCESSING PROBLEMS HARD BRITTLE TISSUE Obvious drying of Drying during all or part of the transport specimen Brittle sections Excessive dehydra that fall out of tion block Microchatter Excessive clearing Excessive heat Shandon Excelsior ES Operator Guide SYMPTOMS POSSIBLE REMEDY CAUSE 1 Use the volume of 20 1 in appro priately sized container 1 Decrease time and or steps 2 Check graduated strengths of alcohol 3 Segregate small delicate specimens 4 Do not use heat and or vacuum 5 Soak for short time in chilled wa ter do not trim away hydrated area and section slowly 1 Decrease time and or steps 2 Evaluate xylene substitutes 3 Segregate small delicate specimens 4 Do not use heat and or vacuum 5 Soak for short time in chilled wa ter do not trim away hydrated area and section slowly 1 Reduce processing temperature during reagent steps 2 Decrease time and or steps 3 Check temperature reading with thermometer 4 Segregate small delicate specimens 5 Soak for shirt time in chilled water do not trim away hydrated area and section slowly Shandon Excelsior ES Operator Guide 101 102 TABLE 4 FREQUENTLY ASKED QUESTIONS
17. return to a previous menu Main Action Sub Menu Press key to select option Press number keys to enter values Standby Help information Highlight item in list J Silence alarm Multi functions Exit from menu e Using a menu is summarised below e Press a number key OR highlight one of the sub menus with the arrow keys Select one of the five options displayed e Press i to show instructions or more information Shandon Excelsior ES Operator Guide Processing in Menu Mode Processing in Daily Operations Mode About Thermo Approved Reagents About Reagents Fixatives About Reagents Dehydrants You can use Main Menu Actions 3 6 for routine processing and reagent replacement This way of using Excelsior ES enables quick modification of program and reagent parameters where needed For the convenience of laboratories not needing flexibility at all times a Daily Operations mode is available where setup program and reagent parameters cannot be changed Return to Menu Mode can be protected by access code This is explained in Chapter 4 Menu Mode Excelsior ES should be installed and used loaded ONLY with reagents from the Thermo approved reagent list on page 119 You should not use any other reagents with Excelsior ES under any circumstances Excelsior ES uses fixative in positions Fix and Fix 2 in the front of the cabinet Two bottles 5 0L are supplied with the ins
18. screen Carry out this check every month BATTERY Check the operation of the switch When the O side ISOLATION is pressed OFF the battery fault icon should be SWITCH displayed When the side of the switch is pressed ON the icon should disappear Carry out this check every month Shandon Excelsior ES Operator Guide Introduction Chapter 3 Instrument Setup Menu You access the Instrument Setup menu from Main Menu when you need complete control of the Excelsior ES configuration Examples are Commissioning a new instrument and loading reagents Complete sections 1 6 as shown on the screen below e Adapting an installed instrument to specific processing needs With Instrument Setup you can change the parameters set above at any time Removing loaded reagents to prepare an instrument for transportation This is explained in section 9 Unload Reagent Groups This chapter describes practical use of the menu sections and the selection keys to achieve associated instrument tasks From Main Menu select 1 Instrument Setup Shandon Excelsior ES Operator Guide 39 1 Set Clock 1 Select 1 Set Clock to display clock settings in the screen ES A Select a key to highlight settings 2 Select SET CLOCK or SET DATE to highlight the settings and display the SET options key Use the number keys to change the parameters When the clock is correct Select SET Select 12 24 HOUR CLOCK and DAY lt gt M
19. 2 Press on the keypad to silence the alarm Shandon Excelsior ES Operator Guide 95 96 OR Using the Fault Status Screen Underfills and Recovery Netmon Session in progress Netmon information is being downloaded and the instrument is being checked If the wrench icon is displayed press on the keypad to display the Fault Status screen below Items monitored More information available The Fault Status Screen shows the following information A numbered list of monitored items Description e Item Status reported as OK WARN or FAIL e The last time each item was monitored Latest Trigger e The number of times there is an issue with any item If the status is WARN you can select ACKNOWLEDGE If the status is FAIL you can select RETRY These selections clear the alert icon and you can try to continue processing If the problem persists contact your Thermo Distributor Reaction chamber underfill and overfill events are displayed here Shandon Excelsior ES Operator Guide BE AWARE OF SAMPLES USED THEY MAY POSE A BIOHAZARD TABLE 1 INSTRUMENT FUNCTION SYMPTOM CAUSE REMEDY Excelsior ES does The instrument is Wait 30 seconds for the initial not respond when still carrying out tests to finish the mains power is initial tests switched on No power supply Connect the power lead Switch on the mains power and instru ment main power switch The mains fuses Replace the main
20. 240 B1000722 6764255 6764250 B1000722 6764262 6764260 6764265 B1000722 9990505 6764506 6764508 9990507 9990509 B1000722 6774060 6774006 501006 502004 Paraplast X tra 1 kg bag 8 503002 A full range of reagents and cassettes is listed in the Thermo catalogue Formal Fixx Glyo Fixx and HistosolveAdobe Garamond Pro are trademarks of Thermo Scientific Paraplast is a registered trademark of Sherwood Medical Peel A Way is a registered trademark of Polysciences Shandon Excelsior ES Operator Guide 117 118 Declaration of Conformity This Declaration of Conformity is only valid when the instrument is used in accordance with the Operator Guide A78410120 Manufacturer s Name Manufacturer s Address Product Description Product Designation Part numbers Year of Marking CE Thermo Shandon Limited Trading as Thermo Fisher Scientific Chadwick Road Astmoor Runcorn Cheshire WA7 1PR ENGLAND Enclosed Tissue Processor Shandon Excelsior ESTM A78400006 including accessories supplied as standard 2002 This product conforms with the essential requirements of the following directives In Vitro Diagnostics Directive Low Voltage Directive 98 79 EC 2006 95 EC This product complies with the following International Standards EMC Safety EN 61326 1997 Inc A1 1998 EN 61000 3 2 2001 EN 61000 3 3 1995 IEC 61010 1 1993 Inc A2 1995 EN 61010 2 010 1994 Inc A1 1996
21. AME To set the access code for the Administrator 1 Highlight Admin Access code not set 2 Select SET ACCESS CODE 3a Enter a four digit code number and press 3b Enter the code again and press You can set Access Codes for different users to define who can perform different functions with Excelsior ES To change the access code groups Enter the Administrator access code on the number keys You can now set up to 7 user groups Shandon Excelsior ES Operator Guide 71 Adding Users To add a user to a group to a Group 1 Use the arrow buttons to move the cursor to the group 2 Select ADD USER 3 The QWERTY keyboard is displayed enter the new user name The name can have up to 5 characters 4 Select EXIT after entering the name to return to the access code screen the new user is now in the group For this user the Administrator can promote demote change name change access code or delete To change the access code Highlight the user you want to change and select CHANGE ACCESS CODE Follow the on screen instructions same as admin change access code and press ENTER To delete a user select DEMOTE to move the user to the lowest user defined group and then select DELETE USER A user has access to the functions of their group and the groups below To enable access codes when the set up is complete press Printout Options The Printout Options screen is shown You can print e Hard copies
22. Control screen Refer to the diagram below for wax loading Wax Bath Inside Door Inside Lever Wax Bath Main Door Waste Wax Tray Main Door Lever Metal Cover 44 Shandon Excelsior ES Operator Guide Wax loading involves e Removing the waste wax tray and metal cover Filling the three wax baths with wax pellets e Replacing the metal cover and waste wax tray this should include liner and lid Starting the wax heater The wax bath main door has two open positions set with the two levers Use the main lever to put the door in the open horizontal door position This lets you move the waste wax tray Use the inclined door position when adding wax pellets 1 Open the wax bath main door to the open position Pull out and remove the waste wax tray and metal cover Pull down the inside door to the open position this lets you return both doors together to the inclined position 4 Attach the wax fill chute as shown Wax Fill Chute Wax Pellets Only i nu EC N Starting from the right carefully fill each wax bath with wax pellets Shandon Excelsior ES Operator Guide 45 Ensure that the wax pellets are evenly distributed in the wax bath push them towards the back if necessary You can fill to within a few millimetres from the top of the dividing wall the pellets melt to the final lower level within approximately 4 hours Take care not to spil
23. E INSTRUMENT ALWAYS ACT WITH COMMON SENSE AND BE AWARE OF THE SAMPLES USED TAKE SUITABLE PRECAUTIONS A A THIS SYMBOL IS USED ON THE EQUIPMENT OR IN A DOCUMENT TO 4 THIS SYMBOL WARNS YOU THAT SURFACES ARE HOT IF THIS SYMBOL APPEARS ON THE INSTRUMENT ALWAYS REFER TO THIS OPERATOR GUIDE A WARN THAT HARMFUL CHEMICALS ARE USED WITH THE INSTRUMENT REFER TO THE MATERIAL SAFETY DATA SHEETS FOR THE CHEMICALS USED ALWAYS ACT WITH COMMON SENSE AND BE AWARE OF LOCAL LABORATORY PROCEDURES TAKE SUITABLE PRECAUTIONS A X THIS SYMBOL IS USED ON THE EOUIPMENT OR IN A DOCUMENT TO WARNING A warning is given in the document if there is a danger of personal injury or damage to samples or eguipment A Note Notes give more information about a job or instruction but do not Jorm part of the instructions A Shandon Excelsior ES Operator Guide How to Use This Guide The Shandon Excelsior ES is intended for use in pathology laboratories by operators familiar with tissue processing technigues and laboratory eguipment Before operating the instrument you should read the section Safety Information on page 8 This Operator Guide is structured to let you start processing guickly and safely with Excelsior ES Chapter 1 Introducing Excelsior ES gives you a tour of the instrument explaining general principles of processing with Excelsior ES You should now be ready to operate the instrument in Daily Operations mode You can learn later
24. Excelsior ES Operator Guide 113 Fuses Switch Conventions Environment Thermo Part Number Centre of Gravity Position External Remote Alarm and Printer to comply with IEC950 or IEC1010 1 Mains plug fuse where applicable 13A 250V Mains input fuses x 2 T10A 250V Remote alarm fuses x 2 F 5A 250V Fuse spares kit A78410028 Note Fuses should only be replaced by technically competent personnel l Power On 0 Power Off Standby General Indoor use only Temperature operation 5 C to 40 C Temperature transit storage 25 C to 55 C 70 C for short expo sure Humidity Maximum 80 RH at 31 C decreasing linearly to 50 RH at 40 C Altitude up to 2000m 6 500 feet Pollution degree 2 Over voltage categoryll A78400006 Centre of gravity position for a fully laden instrument see also page 5 114 Shandon Excelsior ES Operator Guide Accessories Baskets Extraction Adaptor its Filters Monitor Reagent Bottles General INSTRUMENT Organised Basket Random Basket Large Block Metal Divider for Random Basket Basket Lid QUANTITY 6 1 Plastic Basket Carrying Container microwaveable Downdraft Extraction Duct Adaptor Kit Main Air System Duct Adaptor Kit Carbon Potassium Permanganate Filter Potassium Permanganate Filter Monitor Clamp 5 litre Reagent Bottle Wax Waste Tray Kit comprising Wax Waste Tray Wax Waste Tray Lid Wax Waste Liners Door Ke
25. G PRACTICES EXCELSIOR ES WEIGHS APPROXIMATELY 112 KG 246 LB WHEN EMPTY AND 200KG 440LB WHEN FULL AT LEAST TWO PEOPLE ARE NEEDED TO SAFELY MOVE THE INSTRUMENT A 3 Carefully wheel the Shandon Excelsior ES up the ramp until it sits securely in the base Shandon Excelsior ES Operator Guide 123 124 Remove the handles and secure them at the back of the instrument Make sure that the foam pieces are in place Raise the ramp and fit the Inner Cover ATO Secure the ramp to the Inner Cover with tape Fit the Outer Cover Fit the lid over the Outer Cover LL Shandon Excelsior ES Operator Guide 8 Secure the packaging by two bands through the palette and over the lid and one band around the bottom of the packaging WHEN TRANSPORTING EXCELSIOR ES AFTER PACKAGING ENSURE THAT THE INSTRUMENT IS KEPT UPRIGHT AT ALL TIMES A Shandon Excelsior ES Operator Guide 125 Thermo SCIENTI THE RETURN SAFETY DECLARATION Part1 Decontamination Certificate Any instrument or part of any instrument must be clean before being returned and where necessary accompanied by a completed Decontamination Certificate Should the instrument or any part of it be received in an unclean condition or Thermo Fisher Scientific consider it to be a hazard the instrument or part will be returned unrepaired at the expense of the customer It
26. IR B P Time to Reaction Basket eagent rocess E Temperature N Drain Chamber Contra Name Time N Chamber Vacuum rotation Program steps 52 Shandon Excelsior ES Operator Guide Changing Program Step Parameters Hold Use Time e A program can have between 1 and 17 steps To create new programs the suggested seguence is 1 Move to an empty slot in the Program Select screen and produce a new Program title 2 Move to the Define Program screen and highlight your new program Select COPY This displays the list of defined programs from which you can copy the steps Choose a program with similar steps to your requirements You now have a new program title complete with steps which you can change as required Additional steps can be inserted or steps deleted To change the step information Highlight a step The reagent bottle position for the step is highlighted and the bottle outlined e When the Reagent column is highlighted select CHANGE REAGENT to change either the reagent name or the location to be used for that step The Hold Use column shows the temperature for each reagent in its storage location and when in use in the reaction chamber The Hold temperature for concealed reagents and wax can be changed in the Instrument Setup Menu Define Reagent Groups option The Use temperature can be entered here using the keypad The Time column shows hours and minutes for e
27. NNECT AND SWITCH ON UNTIL THE INSTRUMENT HAS BEEN THOROUGHLY DRIED OUT AND CHECKED BY A SERV ICE ENGINEER A Shandon Excelsior ES Operator Guide 105 A A Reagent Bottle Maintenance Fix1 Fix2 Ex1 Ex2 F1 F2 Bottles DO NOT USE ACETONE OR BOUIN S FIXATIVE ANY ACCIDENTAL SPILLAGE OF REAGENTS ON THE KEYPAD SHOULD BE REMOVED BY IMMEDIATELY WIPING WITH A CLOTH AND A SMALL AMOUNT OF ALCOHOL A POTENTIALLY LETHAL VOLTAGES ABOVE 110VAC ARE PRESENT INSIDE THE UNIT DO NOT REMOVE ANY ACCESS COVERS A THE FOLLOWING INSTRUCTIONS ARE THE RECOMMENDATIONS OF THERMO IF ANOTHER CLEANING METHOD IS REQUIRED PLEASE CONTACT THERMO A INSPECT THE INSTRUMENT FOR OBVIOUS DAMAGE OR WEAR WHENEVER YOU CLEAN OR USE IT A REMOVE THE MAINS PLUG FROM THE SUPPLY SOCKET BEFORE YOU CLEAN THE FIXED COMPONENTS OF THE INSTRUMENT A USE GLOVES AND SAFETY GLASSES WHEN DEALING WITH REAGENTS A These are the reagent supplier s bottles in the front cabinet Note that 2 empty 5 litre bottles are supplied for the Fix 1 and Fix 2 positions To remove the bottles carefully remove the dip tubes from the reagent bottles 106 Shandon Excelsior ES Operator Guide Flush 3 Water Bottle A and clean any contamination from the surface of the tubes with a lint free cloth Remove the bottles from the instrument and replace their caps Dispose of the used reagent in accordance with your local regulations and procedures The Flush 3 bottle is in the cab
28. ONTH keys to change the display format AD 40 Shandon Excelsior ES Operator Guide 2 Define Select 2 Define Reagent Groups to display a sub menu with Reagent Groups Storage temperatures for the concealed reagents e Reagent group names Storage temperatures Reagent group names Settings c REP Select to change Select to change storage temperature reagent group name Excelsior ES can heat the concealed reagents during storage for faster more consistent processing The defaults are 30 C for the concealed reagents and 60 C for wax Storage at ambient temperature is available described below To change the storage temperatures Select REAGENT TEMP or WAX STORAGE TEMP e For concealed reagents select AMBIENT or use the number keys up to a maximum of 35 C then press EXIT e Wax is stored in the molten state so the ambient temperature option is not applicable e The available range is 45 C to 65 C e Thermo recommends wax storage temperature 4 C above melting point A Shandon Excelsior ES Operator Guide 41 Note Do not set the temperature of the concealed reagents to more than 15 C above the minimum ambient laboratory temperature A Changing Reagent To change a reagent group name Group Name 1 Select a group DEHYDRANT REAGENT CLEARANT REAGENT or INFILTRATION R A pre defined reagent list is displayed 2 Choose a group name from the displayed list and select SELECT
29. OUESTION ANSWER What is the recom mended way to open the lid during a process run Why is the minimum time in the first wax bath 30 mins How can a step be deleted in a programme Where are the keys to change the alcohol gual ity rotation setting Shandon Excelsior ES Operator Guide Select the lid release key and wait until the status mes sage at the top pg the right hand box on the monitor ing screen says Lid released before opening the lid Note As you have reguested an action you must allow the instrument time to complete it s original activity and then implement the action If you don t do this your subseguent actions might cause you unnecessary problems On no account simply open the lid as this causes and alarm The minimum immersion times for the wax baths are First wax bath 30 minutes Additional wax steps 5 minutes each You need to hold the wax in the chamber for sufficient time to heat the surface of the level sensors If the wax is not hot around the sensor s then when the wax drains out a covering of wax will remain over the sensor The instrument still registers wax up to that level and will therefore keep trying to drain that wax out 30 minutes is the time it takes to ensure that the level of sensors are all up to temperature Use the Delete Step key If you select 0 time in a position Excelsior ES will still draw the fluid into the reaction chamber and then d
30. Process Monitorin Process the tart Select IMMEDIATE START or DELAYED START according to the current program for daytime or overnight processing The monitoring screen is displayed shown below Reagent Current Step Processing Details The status is shown as First Fill Wait Ensure that e The first reagent is drawn into the reaction chamber and the status line changes e The monitoring screen shows that the chamber is filled to the correct level e The baskets are completely covered with reagent Shandon Excelsior ES Operator Guide 25 Starting a Different Process 26 Changing the End Time Changing the End day l PS E Select PROCESS SELECT to display the Process Select screen Highlight the program you want and check that the End Time is correct Select E I sis or DELAYED START Current Day Time Duration of highlighted programme Start End Day Time Select CHANGE END TIME Select SET END TIME Enter the time with the number keys and use the AM PM key if necessary Ensure that End Time and Process are correct Select DELAYED START Select CHANGE END TIME to highlight the time Select SET END TIME Select NEXT DAY on the menu and press the key until Day and Date are correct If you press too many times and go past the day you need you can select PREVIOUS DAY You can set Day and Date up to seven days in advance When End Tim
31. Select DRAIN ALL CHAMBER and wait till draining stops 2 Open the lid and remove basket s If baskets are still shown in the chamber press the LEVEL key to clear them 3 Wipe out the reaction chamber Shandon Excelsior ES Operator Guide 67 You can remove baskets a level at a time after infiltration 1 Select DRAIN NEXT LEVEL and wait till draining stops 2 Open the lid and remove basket s 3 Press LEVEL so the screen shows the number of baskets in the chamber Repeat steps 1 3 until the chamber is empty 4 Wipe out the reaction chamber Flushing and When the screen shows there are no more baskets in the chamber Rinsing the Reaction the Flush screen appears automatically you are required to flush 68 hamber the wax in the reaction chamber before starting the next process 1 Put empty basket s back in the reaction chamber and close the lid 2 From the displayed Flush screen select START For a different Flush program select SELECT FLUSH 3 When the monitoring screen shows the Flush program has finished remove basket s 4 Select OK Excelsior ES is ready to start a new process run Shandon Excelsior ES Operator Guide About the Ouality Control Screen Setting Usage Limits Press QUALITY CONTROL to display the Quality Control screen below It shows e Count number of times reagents and filters have been used e Limit the number of uses before alerts are displayed e Ar
32. Shandon Excelsior ES Operator Guide English A78410120 Issue 3 O 2006 Thermo Fisher Scientific All rights reserved Thermo Shandon Limited is an ISO 9001 and TickIT Accredited Company Thermo Fisher Scientific is the trading name of Thermo Shandon Limited All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries Thermo Fisher Scientific makes every endeavour to ensure that the information contained in its support documentation is correct and clearly stated but does not accept responsibility for any errors or omissions The development of Thermo products and services is continuous Make sure that any published information that you use for reference is up to date and relates to the status of the product If necessary check with Thermo Fisher or your local Thermo representative A This manual may not in whole or in part be copied photocopied reproduced translated or converted to any electronic or machine readable form without prior written consent of Thermo Fisher All information contained in this manual is proprietary and confidential and the exclusive property of Thermo Fisher Scientific This manual is protected by copyright and any reproduction is prohibited This manual is for use only by the individuals to whom it has been made available by Thermo Fisher Scientific Contact addresses Anatomical Pathology Anatomical Pathology 93 96 Chadwick Road 4481 Campus Drive Astmoor Runcorn Kalamazo
33. T Manager 1 Open the upper right door and insert the disc Select DOWNLOAD 3 When the lamp is no longer illuminated the download is complete and you can remove the floppy disc 36 Shandon Excelsior ES Operator Guide Summary You have e Processed your tissue specimens e Flushed the instrument e Replaced fixative and flush reagents e Managed the replacement of the dehydrants clearants and wax across twelve containers using one rotation procedure Shandon Excelsior ES Operator Guide 37 38 Regular The following table describes items for regular checks Maintenance WAX LEVEL Check wax levels in all wax baths BATHS Carry out this check every day LID SEAL Always keep the lid and seal clear of solid wax deposits LATCH Check that the lid latch operates correctly whenever lid is opened The downdraft system should operate and the reaction on screen should show the lid open REAGENT REAGENT When loading or unloading reagents check that the STORAGE TUBES flexible parts of the reagent tubes in the front of the AREA cabinet are not damaged or collapsed REACTION Press the red Heater Trip next to the Reset button in CHAMBER the Reagent Storage Area The Heater Reset switch HEATER TRIP should operate and the reaction chamber Heater trip icon will be displayed on the screen Press the Heater Reset switch fully in to reactivate the reaction chamber heaters The reaction chamber Heater Fault icon will disappear from the
34. W3 are empty whilst Ex1 Ex2 and WW are full as shown 72 Shandon Excelsior ES Operator Guide mmn 1 Replace the Wax a Open the cabinet doors and take out the waste wax tray WW The Quality Control screen appears b Take the second waste wax tray WW supplied with the instrument and put it with a new liner back in the cabinet In the Quality Control screen highlight WW and select CONFIRM EMPTY c Add paraffin wax pellets to the empty wax bath W3 Highlight wax and select START HEATING While the wax is heating go to 2 below 2 Replace alcohol and xylene a Take out containers Ex1 waste alcohol and Ex2 waste xylene and dispose of them b Place fresh 100 96 alcohol and fresh xylene into positions Ex1 and Ex2 In the Quality Control screen highlight the text under alcohol and select NEW Highlight the text under xylene and select NEW 3 Transfer reagents to concealed bottles Before continuing ensure that the wax level is correct Running your next process completes the reagent rotation Shandon Excelsior ES Operator Guide 73 Reagent Rotation Examples of reagent rotation procedures are described here for Example 2 rotating reagents as directed by the Quality Control screen Control All Groups The timeline assumes a schedule of one process run per day DAY 1 The alcohol quality has reached its defined limit and a triangle is displayed above the alcohol quality indicator The Quality Cont
35. about reagent management programming and customising Fxcelsior ES Chapter 2 Daily Operations Mode shows you how to run defined processs programs replace and rotate reagents These are with current settings Chapter 3 Instrument Setup Menu gives details of Excelsior ES programming reagent loading and customisation Chapter 4 Menu Mode explains using Excelsior ES with all options available Chapter 5 Installing Excelsior ES shows you how to install the instrument on site and correctly connect to an electrical supply Chapter 6 Troubleshooting explains alert symbols and gives instructions to clear them using the Fault Status screen The reference lists suggest solutions to common processing issues Shandon Excelsior ES Operator Guide Safety Information THIS SECTION DETAILS IMPORTANT SAFETY INFORMATION PLEASE READ ALL OF THIS SECTION CAREFULLY Thermo products are designed for convenient and reliable operation and to accepted standards of safety Their use does not entail any hazard if operated in accordance with the instructions given in this manual However incorrect actions by user may damage this equipment or cause a hazard to health It is important for you to obey the following safety precautions All users must read and understand the Operator Guide and only operate the unit in accordance with the instructions If the instructions are not followed then the protection provided by the instrument may be impaired
36. ach processing step the maximum is 99 59 Highlight the time and change by entering a number with the number keys Shandon Excelsior ES Operator Guide 53 Notes 1 The time to transfer reagents is included in this time Steps should be at least 3 minutes long If a shorter step is entered and fluid transfers take longer than 3 minutes then the program will overrun its expected end time A 2 The first wax step in any program should be at least 30 minutes long in order to minimise wax carry over on the reaction chamber walls and to allow the level sensors to warm up to the wax temperature A 3 The Drain column shows a time in seconds to allow the specimens to drain before moving to the next processing step Vac The Vac column controls the pressure in the reaction chamber during each step Select VACUUM ON to process at approximately 650 mBar absolute 350 mBar below atmospheric pressure VACUUM OFF to process at atmospheric pressure VACUUM CYCLING for a pressure increase decrease cycle every 15 minutes Stir The Stir column controls the Contra stir rotation Note Select Stir setting 3 for the equivalent of Thermo Shandon Pathcentre Contra stir A Copying Step 1 From the Define Program screen Select COPY to display Parameters the list of defined programs 2 Highlight the required program from the list When the correct program is highlighted selecc COPY NOW Select RESTORE DEFAULTS
37. affin wax pellets to the empty wax bath W3 Highlight wax and select START HEATING While the wax is heating go to 2 below 2 Replace alcohol a Take out container Ex1 waste alcohol and dispose of b Place fresh 100 alcohol into position Ex1 In the Ouality Control screen highlight the text under alcohol and select NEW 3 Before continuing ensure that the wax level is correct Running your next process completes the reagent rotation Shandon Excelsior ES Operator Guide 75 7 Options From Main Menu Select 7 Options to display the Options Menu screen Daily Operations This takes you to Daily Operations mode Select Language Highlight the language you want and select SELECT Set Time and Date Sets Time and Date as in Chapter 3 Instrument Setup The Access Codes screen is shown below 76 Shandon Excelsior ES Operator Guide Administrator Access Code With no functions protected operators can move between menus and options without restriction Access Code protection is available for the following functions Change reagents Enter Menu mode Start a program run Step a program Override OC warnings Edit programs e Change options You have to e Use PROMOTE to move and prioritise functions Use ADD USER to assign users to the functions The first function Edit Access Groups is assigned to the default user name Admin This is the Administrator The name can be changed using CHANGE N
38. agents Reagent Rotation Options Shandon Excelsior ES Operator Guide Appendix A Appendix B Appendix C Appendix D Installing Excelsior ES 85 Introduction Unpacking Moving Levelling Connecting Monitor Printer Fitting Filters Connecting to Mains Power Powering ON and OFF Standby Troubleshooting 95 Introduction Alert Icons The Fault Status Screen Underfill Recovery Problem Cause Remedy Tables and Frequently Asked Questions Cleaning and Maintenance 105 Specification and Accessories 113 Specification Accessories Part Numbers Ordering Reagents Declaration of Conformity Approved Reagent List 119 Introduction Fixatives Dehydrants Clearants Infiltrants Flush Reagents Cleaning Agents Program Examples 12 Routine Overnight Program Rapid Biopsy Program Standard Flush Program Extended Flush Program Rinse Program Transportation Instructions 123 Packaging the Instrument Product Return Safety Declaration Warranty Statement Index 128 Shandon Excelsior ES Operator Guide Shandon Excelsior ES Operator Guide Symbols The following symbols and conventions are used throughout this manual and on the instrument WARN THAT INSTRUCTIONS MUST BE FOLLOWED FOR SAFE AND CORRECT OPERATION IF THIS SYMBOL APPEARS ON THE INSTRUMENT ALWAYS REFER TO THIS OPERATOR GUIDE A THIS SYMBOL IS USED ON THE EQUIPMENT OR IN ADOCUMENT TO WARN THAT THERE MAY BE A BIOHAZARD ASSOCIATED WITH TH
39. am 121 Standard Flush Program 122 Extraction filters fitting 87 F Fault Status screen 97 Filters 87 extraction filters upper filter potassium permanganate 88 lower filter carbon 88 Fixative approved reagents 20 Flush preparing chamber for first use 50 Flush program standard extended 28 68 Fuse mains plug 90 mains input 114 H Heater reset switch 91 Height 113 l Information button 19 Inspect reagent and wax checks 98 Installing Excelsior connecting monitor 87 electrical requirements wiring details 91 fitting filters extraction filters lower filter carbon 88 upper filter potassium permanganate 88 introduction 63 85 95 105 108 119 123 Instrument Depth 113 Instrument Height 113 Instrument Weight 9 113 Instrument Width 113 L Levelling instrument 86 LIMS interface 113 Loading reagents fixatives 48 fixed reagents 49 50 89 Maintenance 10 regular 105 Monitor 87 Notes add Notes feature 52 Shandon Excelsior ES Operator Guide 129 0 organised baskets 16 17 p Physical Specification 113 Pinchpoint symbol Al 6 Powering OFF the instrument 103 Powering the instrument OFF 93 ON 91 Power Supply Requirements 113 114 Printout options 78 Processing Problems troubleshooting 98 Hard Brittle Tissue 101 Soft Spongy Tissue 100 Product Return Safety Declaration 126 Programs rapid biopsy 121 routine overnight 121 Program select Program sele
40. and 2 are shown indicating normally open and normally closed connectors Shandon Excelsior ES Operator Guide 87 Fitting Air Extraction Fitters BOTH FXTRACTION FILTERS MUST BE FITTED DO NOT OPERATE THE INSTRUMENT IF EITHER EXTRACTION FILTER IS NOT FITTED A FOR CORRECT OPERATION OF THE EXTRACTION SYSTEM MAKE SURE THAT THE UPPER AND LOWER DOORS ARE CLOSED A Excelsior ES is delivered with new filters fitted into the instrument Before using the instrument e Remove the filters Remove the plastic wrappers e Replace the filters The two main extraction filters separated by a removable metal baflle are behind the top door The upper filter uses potassium permanganate to extract formalin vapours The lower filter uses carbon to extract solvent vapours To remove the filters 1 Open the top door and remove the metal baffle plate 2 Slide out the filters and remove the plastic wrappers 3 Replace the filters in the correct positions so that the airflow arrow on the filter points up 4 Replace the baffle plate and close the top door Upper Filter Baffle Lower Filter Shandon Excelsior ES Operator Guide Downdraft Filter Fitting Optional Vent Adaptors Note 1 The filters fit tightly into their slots for efficient air extraction The downdraft filter potassium permanganate is at the back of the instrument behind the reaction chamber 1 Remove the filter s
41. ard the used reagent in accordance with local procedures and regulations When the reaction chamber is empty e The screen highlights the empty bottle The option LOAD REAGENT is shown To load a new reagent 1 Place a new reagent bottle in position Ex1 or Ex2 and highlight the empty bottle 2 Select LOAD REAGENT and follow the screen instructions Using Inspect to You can also rotate a reagent into an empty concealed reagent Rotate a Reagent bottle by inspecting the reagent in the next concealed bottle for that reagent group When you inspect the next bottle the options include RETURN REAGENT DISCARD REAGENT and ROTATE AND RETURN REAGENT If you select ROTATE AND RETURN REAGENT the reagent is emptied into the original concealed bottle now empty You can then fill that empty bottle with new reagent from the exchange bottle or rotate the reagent from the next concealed bottle A Note After inspecting xylene or equivalent reagents the chamber must be flushed before processing Inspecting a Wax Highlight the relevant wax bath Select INSPECT WAX to move the wax into the reaction chamber 82 Shandon Excelsior ES Operator Guide To return the wax to the wax bath press RETURN WAX Note 1 The wax must be present for a minimum of 30 minutes in the reaction chamber before returning to the wax bath A 2 If wax is drawn into the reaction chamber a flush must be run A To discard all the exist
42. ast step for those process groups which have been rotated Shandon Excelsior ES Operator Guide 71 Reagent Rotation An example procedure is described here the feature Control All Example 1 Groups in the Quality Control screen enables alcohol in A1 xylene in X1 and wax in W1 to be replaced together The timeline assumes a schedule of one process run per day DAY 1 The alcohol quality has reached its defined limit and a triangle is displayed above the alcohol quality indicator The Quality Control screen is displayed automatically when the next processing run is due to begin 1 a In the Quality Control screen highlight the text Control All Groups The text below alcohol and xylene is Rotation Due next Run The text below wax is Discard Due next Run The selection key options are ACKNOWLEDGE ALL and POSTPONE ALL Select ACKNOWLEDGE ALL 2 Open the doors to ensure that containers Ex1 Ex2 and WW are empty waste wax tray WW should have a liner 3 Run your next process to continue with the rotation Excelsior ES uses reagents as usual from Al X1 and W1 discarding them into the exchange positions and the waste wax tray After the process steps using reagents A2 X2 and W2 these are not returned to these positions they are moved forward by one position and become the new reagents for Al X1 and W1 This continues for all subsequent reagents DAY 2 The next morning the screen indicates A6 X3 and
43. at the I O power switch at the rear of the instrument is switched off O side of the switch pushed IN Instruments are supplied with power cords with moulded plugs suitable for many countries If another plug is required it is necessary for a technically competent person to remove the moulded plug from the white power cord UK style plug and fit a suitably rated fused plug using the wiring convention shown below Shandon Excelsior ES Operator Guide Powering the Instrument ON Live L or L2 terminal Neutral N or L1 terminal Green yellow wire Earth terminal E ground or V Insert the appropriate mains cable into the mains connector on the rear panel of the instrument and clip the cable restraint over the mains connector Connect the mains supply cable to the local power supply outlet EXCELSIOR ES MUST BE ELECTRICALLY EARTHED MAKE SURE THAT THE INSTRUMENT IS PLUGGED INTO A PROPERLY EARTHED GROUNDED MAINS SUPPLY A IT MUST BE POSSIBLE TO INTERRUPT THE MAINS SUPPLY AT SOURCE BY REMOVING THE PLUG FROM THE MAINS SUPPLY SOCKET A Press the I ON side of the I O power switch When Excelsior ES is powered ON you should notice the following 1 The cooling fans audibly start and the instrument LED lamps illuminate 2 The rotary valve motor audibly starts 3 After 30 seconds the language screen is displayed Highlight the required
44. ct NEXT DAY on the menu and press the key until Day and Date are correct If you press too many times and go past the day you need you can select PREVIOUS DAY You can set Day and Date up to seven days in advance When End Time and Day are correct select DELAYED START Shandon Excelsior ES Operator Guide Adding Specimens After Starting Process No Baskets Added Baskets Added Aborting a Process When Processing is Complete Removing Specimens You can add tissue cassettes after a process has started 1 Select LID RELEASE Wait for the downdraft fan to start and for the vacuum to release if the step uses vacuum 2 Open the lid add cassettes or baskets and then close the lid Follow either 3a or 3b below 3a In the event that you do not add more baskets or are using a random basket simply select RESTART This continues processing to the original fill level 3b If you do add additional baskets use the LEVEL key accordingly so that the basket level shown on the screen corresponds with the baskets in the chamber Then select REFILL AND RESTART From the monitoring screen 1 Select STOP 2 Select ABORT The process is stopped and the reaction chamber drains The status line on the monitoring screen displays Process Complete You have to e Drain the reaction chamber e Remove baskets e Wipe away excess wax from the chamber lid and seal e Flush and rinse the chamber 1
45. ct screen 53 Q Quality Control override QC warnings 77 Reaction chamber heater trip 96 Reagent rotation 32 72 control all groups 34 74 rotation management 57 Shandon Excelsior ES Operator Guide Reagent storage area 38 Reagent Tubes 47 Regular maintenance 38 Removing specimens Drain options 28 67 Rinse program 122 S Safety information 8 Set LIMS interface 80 SPECIFICATION AND ACCESSORIES Accessories Instrument accessories 115 baskets 115 extraction adaptor kits 115 Reagents 116 Specification 114 Centre of Gravity Positions 114 Electrical 114 Environment 114 Part Numbers 114 Physical 114 Switch Convention 114 T Transportation instructions 123 Troubleshooting instrument function table 1 98 processing problems hard brittle tissue table 3 101 increasing process time 100 soft spongy tissue table 2 100 U X Underfill Xylene alert displayed 99 loading clearants 49 User access groups 77 GOP substitute ordering 116 V Vacuum release knob 99 Vent adaptors 89 W Warranty statement 127 Waste wax 21 32 34 72 74 liner 45 Wax loading wax 44 melting wax 46 wax bath heater 46 paraffin wax pellets 21 adding 33 35 73 75 storage temperature 41 waste wax container WW removing 32 34 72 74 wax rotation 32 72 Wax Waste Tray 108 Wiring details 91 Shandon Excelsior ES Operator Guide 131
46. e Waste Wax Tray after reaching the usage limit Fixative and Flush Reagent Bottles are in the front of the cabinet Two Exchange Bottles are stored empty ready for reagent exchange operations involving the concealed bottles The concealed Dehydrant and Clearant Reagent Bottles fixed within are NOT accessible to operators The Electrical Connections panel is for mains power and monitor optional printer and remote alarms data communications and the instrument POWER ON OFF switch Shandon Excelsior ES Operator Guide 15 Systems Monitoring To assure processing guality and specimen security Excelsior ES constantly monitors its systems and continuously updates the displayed status When you see the Thermo logo displayed at the top of the screen this means all systems are OK If Excelsior ES detects that part of the system is not OK the Thermo logo is replaced by one or more alert icons Systems OK ALERT The icons can indicate the need for reagent management and maintenance tasks hardware issues and instrument faults See Chapter 6 Troubleshooting for more information 16 Shandon Excelsior ES Operator Guide How You Process You can put specimen cassettes into organised baskets With Excelsior ES arranged in pairs Shandon Excelsior ES Operator Guide 17 You can process up to three Levels of organised baskets arranged in pairs For larger specimens use a random basket Up to 3 Levels of or
47. e and Day are correct select DELAYED START Shandon Excelsior ES Operator Guide Adding Specimens After Starting Process No Baskets Added Baskets Added Aborting a Process When Processing is Complete You can add tissue cassettes after a process has started 1 Select LID RELEASE Wait for the downdraft fan to start and for the vacuum to release if the step uses vacuum 2 Open the lid add cassettes or baskets then close the lid Follow either 3 or 4 below 3 In the event that you do not add more baskets or are using a random basket simply select RESTART This continues processing to the original fill level 4 If you do add additional baskets and the LEVEL key is enabled press the LEVEL key accordingly The basket level shown on the screen must correspond with the baskets in the chamber Then select REFILL AND RESTART Note With the LEVEL key enabled and Restart set to ON the chamber is refilled to the random basket level For more information on Level and Restart options see Chapter 3 Instrument Setup Customisation page 51 From the monitoring screen 1 Select STOP 2 Select ABORT The process is stopped and the reaction chamber drains The status line on the monitoring screen displays Process Complete You have to Drain the reaction chamber Remove baskets Wipe away excess wax from the chamber lid and seal Flush and rinse the chamber Shandon Excelsior ES Operator
48. e reaction cham ber is empty You have selected New Notes Each Run on the pro gramme screen The lid is not fully closed The emergency vacuum release has been removed The pipe is not fully intersected in the bottle This is correct these bottles are empty after a discard run The reagents from A6 and X3 have been rotated to A2 and X2 The tray has not been detected Go to Reagent and Wax Checks 9 and confirm there are no reagent transfers in progress In spect a reagent for example A1 then return it to the bottle Go to Edit Programme and deselect this option if you don t want it Open and close the lid press on either side of the knob to close the lid do not use the knob itself Refit the red vacuum release knob behind the panel between the two front filters Ensure the pipe reaches to the bottom of the bottle and is not bent or twisted Remove the discarded reagents and replace them with the new 10096 alcohol and xylene in the Ex1 and Ex2 positions respec tively After the next run A6 and X3 are filled with new reagents 1 Go to the Quality Control Screen and confirm that the tray has not registered 2 Find the reagent position 3 From Main Menu use Reagent and Wax Checks to cor rectly fill the reagent bottle If there is an instrument malfunction remove ALL specimens from the reaction chamber If it is necessary to release the chamber vacuum open the top rig
49. e use Fit the transport handles and carefully tilt the instrument back and push it on the rear wheels Do not tilt the instrument when it is loaded with reagents Move the instrument carefully on all four castors ALWAYS KEEP THE INSTRUMENT LEVEL WHEN IT IS LOADED WITH WAX AND REAGENTS MOVE IT SLOWLY TO PREVENT WAX SPILLAGE A ALWAYS DISCONNECT AND REMOVE THE MONITOR BEFORE THE INSTRUMENT IS MOVED A Shandon Excelsior ES Operator Guide 85 Levelling the Instrument Ensure that you have all parts on the packing list If parts are missing or broken contact your dealer Notes 1 Ouote the instrument Serial Number your Order Number Invoice Number Delivery Note or Packing Slip Number and the date 2 If you need to transport the instrument refer to Appendix D for repacking instructions Excelsior ES must be level from front to back Before loading with reagents and wax ensure that it is fully adjusted EXCELSIOR ES WEIGHS APPROXIMATELY 112 kg 246 Ib WHEN EMPTY WHEN FILLED WITH REAGENTS AND WAX Excelsior ES WEIGHS 200 kg 440 Ib A Move Excelsior ES to its final position The floor should be level the floor and any covering must be non flammable There should be at least 95 mm 4 inches behind the instrument To ensure this leave the transit handles fitted Open the reaction chamber lid and remove the dessicant pack from the chamber Pour 50ml of water into the reaction chamber and use it as a fluid le
50. ent rotation e Unloading of reagents When the demonstration is running the screen background is pink and the Demo icon is displayed to indicate clearly that no real processing is being shown You can watch the demonstration at any time even during processing Fast Forward and Fast Fast Forward options let you speed up the demonstration To exit the demonstration press on the keypad This takes you to Daily Operations mode If the instrument has been set up with an access code for Menu Mode this has to be entered to return to Menu Mode e The Unload screen is only available when reagents have already been loaded into Excelsior ES e Follow the instructions on the Unload screen and ensure the waste wax tray is correctly fitted Select UNLOAD to pump the wax from the selected wax bath into the reaction chamber and then discard it into the waste wax tray e Repeat this process for the other wax baths e When the last wax has been unloaded the screen instructions will prompt you to select START for a flush run Shandon Excelsior ES Operator Guide 59 e This will remove any remaining wax from the reaction chamber e The unload procedure will continue by highlighting the current clearant bottle and the Ex 2 position bottle e Confirm that bottle Ex 2 is empty and select UNLOAD to continue to remove the reagents e Remove bottle Ex 2 and dispose of in accordance with local procedures and regulations e P
51. erated or maintained in accordance with the instructions in this Operator Guide Shandon Excelsior ES Operator Guide 127 Index Symbols A biohazard symbol 6 E harmful chemical symbol 6 A pinchpoint symbol 6 N warning symbol 1 6 sss w mua warning symbol 2 6 A Access codes 77 Administrator 77 Agitation 15 drive pins 24 64 Alarms remote audio 80 silence alarm button 80 96 Arrow keys 19 B Biohazard symbol A 6 C Centre of Gravity 114 Changing a program title 51 Clearant approved 119 position 20 Conformity declaration of 118 contact addresses 2 CONTROLS AND SCREEN LAYOUT Screen Displays Shandon Excelsior ES Operator Guide Information Icons Quality Control Warning 96 Remote Alarm 96 Customisation 43 Daily Operations processing overview 20 Declaration of Conformity 118 Define Program screen 53 program steps 54 Defining reagent groups 41 reagent temperature 41 Dehydrants loading 49 Dehydrant Reagent 42 Disk drive options 79 Downdraft vent adaptor 90 Downdraft filter fitting 89 Drain drain wax 82 Drain options 28 67 E Earthing Grounding Details 8 Edit program 51 add notes 51 change notes 52 drain 54 setting reaction chamber vacuum 54 Electrical Specification 113 114 Emergency vacuum release 99 Environmental conditions 114 EXAMPLE ROUTINES Extended Flush Program 122 Rapid Biopsy Program 121 Rinse Program 122 Routine Overnight Progr
52. erator Guide 13 Excelsior ES Instrument Tour Ca J zi Screen Keypad I e Disc Dri Reaction Chamber ED Extraction Filters D 0 Emergency Vacuum J Release is Waste Wax Tray L Switches INFILTRANT Wax Baths FLUSH Reagent Bottles FIXATIVE Reagent Bottles Exchange Bottles i Downdraft Filter Electrical Connections DEHYDRANT and CLEARANT Bottles 14 Shandon Excelsior ES Operator Guide The Reaction Chamber is where processing of tissue specimens takes place according to defined programs When the lid is open fumes are extracted through the Downdraft Filter The Screen has a menu window and a real time graphic display and the Keypad is used to operate Excelsior ES Five keys select menu options with number and arrow keys for other controls The Disc Drive uses standard floppy discs for Excelsior ES data transfer options Two Extraction Filters clean the cabinet air this helps to contain fumes within the instrument Behind the baffle plate the Vacuum Release Knob is for emergency situations only Switches inside the cabinet let you test and reset the heater and isolate the battery The Wax Baths store the infiltrant reagent which is discarded into th
53. es with absorbent paper and small amount of xylene THE LID SEAL AND TOP SURFACE OF THE REACTION CHAM BER MUST BE KEPT CLEAR OF WAX FOR THE INSTRUMENT TO OPERATE CORRECTLY THE LID SEAL CANNOT BE REMOVED FOR CLEANING DO NOT TRY TO REMOVE IT Shandon Excelsior ES Operator Guide 109 110 Cleaning the Monitor Use absorbent paper to wipe out any residual wax from the Reaction Chamber Note Flush reagents will last longer if wax is removed from the Chamber and Baskets before a flush is carried out 2 Use absorbent paper to gently wipe the 4 level sensors in the Reaction Chamber DO NOT use abrasive cleaners 3 Do not use metal tools to clean or scrape the reaction chamber 4 Do not use any chemicals other than those recommended in Appendix C 5 Do not scrape around the edge of the reaction chamber base c If any debris falls into this gap carefully use tweezers to remove it 6 Close the Reaction Chamber Lid and perform a flush to rinse the Cham ber Note 1 Wipe the Reaction Chamber after every flush and check for any contami nation Evidence of wax may indicate that the flush reagents need to be changed 2 Ensure the instrument is powered OFF Disconnect the monitor from the instrument Wipe the monitor gently with a soft damp cloth Shandon Excelsior ES Operator Guide WARNING Changing Filters Spillages 3 Donotuse solvents to clean the monitor Change the filters every 3 months To
54. f dehydrants the reaction chamber must be flushed as prompted by the START option key displayed Select START This involves Filling the empty Ex1 bottle with dehydrant at each concentration as highlighted on the Load Reagents screen e Transferring the reagent from Ex1 into the reaction chamber and then into the concealed bottle Bottle Al is outlined on the screen 1 Filla bottle between 3 8 L and 5 0 L with alcohol diluted to 75 2 Put the bottle in position Ex1 and insert the BLUE tube with cap 3 Select CONFIRM LOADED to start the dehydrant reagent transfer The next bottle A2 is outlined on the screen Repeat the above steps to load dehydrants A2 A6 Load A2 with 90 alcohol Load A3 with 95 alcohol Load A4 A6 with 100 96 alcohol This involves e Placing a bottle of clearant in position Ex 2 Transferring the reagent from Ex 2 into the reaction chamber and then into the concealed bottle If xylene is used as the clearant X1 Xylene is highlighted and bottle X1 outlined on the screen 1 Put the bottle of clearant in position Ex2 and insert the RED tube with cap 2 Select CONFIRM LOADED to start the clearant reagent transfer Repeat the above steps to load clearants X 2 and X 3 Shandon Excelsior ES Operator Guide 49 When all the clearants are loaded you are prompted to start a flush cycle to prepare the reaction chamber for use To do this select START When the fl
55. formation is correct press to return to the previous screen This section sets the trigger for reagent rotation and automatic display of the Quality Control screen at the start of a process This is for Excelsior ES to inform the operator e The defined reagent usage limit has been reached e They should rotate reagents Shandon Excelsior ES Operator Guide 57 58 3 Reagent groups triggered The Rotation Management default setting is shown below to prompt for rotation by alcohol guality in A1 Arrow across settings gt You scroll up and down the rotation modes Scroll across to display rotation options for a reagent group When the alcohol guality falls below the set level the Ouality Control screen is displayed before you start processing and you are prompted to rotate reagents To use reagent counting enter a number under Rotation Usage Limits to trigger prompting from process counts Highlight a day of the week if you want to rotate reagents according to the day selected It is possible to set more than one trigger but for correct use of the system you should only set Excelsior ES for one of the rotation trigger options Shandon Excelsior ES Operator Guide 7 Concept Demonstration 8 Daily Operations 9 Unload Reagent Groups The concept demonstration shows you reagent movements during tasks performed with Excelsior ES including e Loading reagents e Processing e Flushing e Reag
56. ganised baskets mm s Random basket A N N NISN l PU W m ALAN A B D PIIN N NNN You put the baskets into the reaction chamber covering the specimens with a basket lid 18 Shandon Excelsior ES Operator Guide e You select a start option on the keypad When you start a program Excelsior ES moves through the process steps drawing in reagents in turn and agitating the baskets to stir reagent around the specimens The instrument can be set up to heat the chamber and put the chamber under constant or cycled vacuum conditions for process steps This is described in Chapter 3 Instrument Setup About the Excelsior ES presents a uniquely compact efficient and highly Excelsior ES informative operator interface showing Interface e Reaction chamber status e Program details and menu text e Option keys e Animated graphics showing processing and reagent movement in real time e On screen help information Customer Information Alters Logo Reaction Chamber Alcohol Quality Wax N ahi Reagents mL LAJIA ptions Shandon Excelsior ES Operator Guide 19 20 You press one of the five keys to select an option The options are grouped according to the instrument mode and displayed menu The interface lets you perform tasks intuitively efficeintly and consistently e Press Exit Jto close a menu and
57. he bottle e Not bent twisted or kinked Shandon Excelsior ES Operator Guide 47 Loading Flush Flush reagents must be loaded in the order F 3 F 1 F 2 Reagents F 3 Flush 3 should be highlighted first on the screen When removing bottle caps keep them safely they are needed when the reagent is changed FLUSH 3 IS A WATER RINSE USED DURING THE LOAD ROUTINE AND FOR THE THIRD FLUSH REAGENT A DO NOT USE XYLENE AS THE THIRD FLUSH REAGENT A LOADING FLUSH 3 1 Fill the Flush 3 bottle supplied with Excelsior ES to the bottom of the neck with water 2 Replace the cap securely and put the GREEN reagent tube into the bottle 3 Select CONFIRM LOADED The screen moves to F1 Flush 1 highlighting the text and outlining the bottle In positions F1 and F2 Excelsior ES uses manufacturers reagent bottles LOADING FLUSH 1 1 Take a new bottle 3 8 5 litres of the first flush reagent e g xylene 2 Place the bottle on top of the Flush 3 bottle and insert the RED reagent tube with cap 3 Select CONFIRM LOADED LOADING FLUSH 2 The screen moves to F2 Flush 2 highlighting the text and outlining the bottle 1 Take a new bottle of the second flush reagent e g alcohol 2 Put the bottle on top of the Flush 3 bottle in front of bottle F1 and insert the BLUE reagent tube with cap 3 Select CONFIRM LOADED 48 Shandon Excelsior ES Operator Guide Loading Dehydrants Loading Clearants Before loading o
58. hecklist below to ensure that the instrument being returned is ready for collection All reagents wax removed from instrument including vapour traps if applicable Accessories are secured itemised Instrument has had transit clamps fitted as per Operator Guide if applicable Instrument is packed in original packaging RMA NUMBER CARRIER FOR ATTENTION OF Thermo Fisher Scientific Anatomical Pathology 4481 Campus Drive Kalamazoo MI 49008 USA Tel 1 800 522 7270 Fax 1 269 372 2674 www thermo com pathology Thermo Fisher Scientific Anatomical Pathology 93 96 Chadwick Road Astmoor Runcorn Cheshire WA7 1PR UK Tel 44 0 1928 562600 Fax 444 0 1928 562627 www thermo com pathology 126 Shandon Excelsior ES Operator Guide WARRANTY STATEMENT We are proud of our guality and reliability and of our after sales service We continuously strive to improve our service to our customers Please ask your distributor or representative about Service Contracts which can keep your purchase in peak condition for many years to come Warranty provisions necessarily vary to comply with differences in national and regional legislation and you can find details in your delivery documents or from your dealer or representative Please note that your warranty may be invalidated if e The instrument is modified in any way Accessories and reagents are used that are not approved by Thermo or The instrument is not op
59. ht door remove the metal plate and pull the vacuum release knob see pgs 14 15 Contact Thermo Service Shandon Excelsior ES Operator Guide TABLE 2 PROCESSING PROBLEMS SOFT SPONGY TISSUE SYMPTOMS POSSIBLE CAUSE REMEDY Difficult to section Section breaks up on the water bath Poor staining Clearing impossible Discoloured cloudy areas within specimen Section breaks up on water bath Wax cannot infiltrate Discoloured cloudy areas within specimen Section breaks up on the water bath Can feel or smell clear ing solution No support when sec tioned Section breaks up on the water bath Inadeguate fixation Inadeguate dehydra tion Inadeguate cleaning Inadeguate wax infil tration 1 Increase time in fixative 2 Use microwave enhanced technigue 3 Bisect if encapsulated speci men or produce a thinner block Note that improper fixation cannot be remedied 1 Increase time in alcohol 2 Make sure concentration of alcohol is correct 3 Adjust solution rotation schedule 4 Rule out contamination from lipids 5 Bisect if encapsulated speci men or produce a thinner block 6 Reinfiltrate in wx If inad eguate reverse process to abso lute alcohol and reprocess 1 Make sure the specimen is completely dehydrated 2 Adjust rotation schedule 3 Increase time in clearant 4 Use vacuum 5 Bisect if encapsulated speci men or produce a thinner block 6 Reinfiltrate in wax If inadeguate reverse
60. inet below the Flush 1 and Flush 2 bottles To remove Flush 3 water bottle first remove the Flush 1 and Flush 2 bottles Remove the green reagent tube from the rear of the bottle and carefully lift and slide the bottle out of the Reagent Storage Area Remove the cap and dispose of the water in accordance with any local procedures and regulations KEEP THE FLUSH 3 BOTTLE LEVEL TO AVOID ANY SPILLAGE A THE FLUSH 3 WATER BOTTLE MUST BE CLEANFD BEFORE IT IS REFILLED WITH WATER A To clean the Flush 3 water bottle rinse the bottle with clean water and mild detergent Use a bottle brush if reguired A DO NOT USE TEMPERATURES HIGHER THAN 60 C A Refill the Flush 3 bottle with water to the bottom of its neck and replace the cap Place the water bottle in the Reagent Storage Area with the cap towards the front of the instrument Fit the green drain tube into the open pipe at the rear of the Flush 3 Water Bottle Make sure the tube is fully inserted into the Water Bottle so that it sits on the bottom of the bottle and that there are no kinks in the flexible section Shandon Excelsior ES Operator Guide 107 108 Discarding Used Wax of tube REFER TO THE MATERIAL SAFETY DATA SHEETS MSDS WHEN HANDLING THE REAGENTS LISTED IN APPENDIX C A When the wax is changed using the rotation technigue as described the oldest wax is discarded into the waste wax tray Excelsior ES prompts you to confirm that the waste wax tray is in
61. ing wax and to start again with fresh wax press DISCARD ALL or to discard some of the wax to top up with fresh wax press DISCARD SOME The screen instructions ask you to confirm that the wax waste tray is empty Select CONFIRM LOADED to drain the wax from the reaction chamber to the wax waste tray Discard the used wax in accordance with local procedures and regulations Shandon Excelsior ES Operator Guide 83 When the reaction chamber is empty the screen highlights the empty wax bath and the second key shows START HEATING Pour new wax pellets into the wax tray and press START HEATING 84 Shandon Excelsior ES Operator Guide Introduction Unpacking Moving the Instrument when Empty Moving the Instrument when Loaded Chapter 5 Installing Excelsior ES This chapter includes Unpacking and moving the instrument Levelling the instrument Connecting the monitor Fitting the filters and vent adaptors Making mains power connections Powering the instrument ON USE SAFE LIFTING PRACTICES WHEN MOVING Excelsior ES Excelsior ES WEIGHS APPROXIMATELY 112 kg 246 lb WHEN EMPTY AND 200 kg 440 Ib WHEN FULL AT LEAST TWO PEOPLE ARE NEEDED TO SAFELY MOVE THE INSTRUMENT A Inspect the packaging If it is damaged inform your dealer and inspect the instrument after unpacking Remove the banding and lid from the packaging Follow the instructions on the packing case Do not discard the packaging store it flat for futur
62. l any wax pellets RISK OF SKIN BURNS FROM MOLTEN WAX A DO NOT FILL THE EXCELSIOR ES WAX BATHS WITH MOLTEN WAX USE WAX PELLETS ONLY A Wax bath W1 is highlighted on the screen 5 Select WAXBATH HEATER ON to switch on the wax bath heaters and melt the wax pellets 6 Follow the screen instructions and repeat the key selections for wax baths W2 and W3 7 When the wax has melted ensure that the level reaches the top of the interior walls of the wax baths 8 If the level is too low add more wax pellets 9 Fit a new liner into the waste wax tray then replace the metal wax cover waste wax tray and lid 10 Close the wax bath main door Waste Wali gt Liner Waste Wax Tray 46 Shandon Excelsior ES Operator Guide Excelsior ES is now ready for loading the rest of the reagents REFER TO THE MATERIAL SAFETY DATA SHEETS MSDS WHEN HANDLING ALL REAGENTS USED WITH THE INSTRUMENT A ENSURE THAT THE COLOUR CODED REAGENT TUBES ARE FULLY INSERTED INTO THE BOTTLES A About Colour Coded The diagram below shows the colour of the tube for each reagent Reagent Tubes position Fix1 Fix2 Ex2 Ex1 F2 FI Fixative Fixative Clearan it Clearan t Flush Flush Left Front Left Back Exchange Exchange Right Front Right Back GREEN GREEN Centre Back Centre Front BLUE RED e RED BLUE 6 i otto Ensure that each pipe is e Fully inserted in t
63. lace another empty bottle in the Ex 2 position and follow the screen instructions to remove the remaining clearant reagents e When the last clearant reagent has been unloaded the screen instructions will prompt you to select START for another flush run e This will remove any remaining clearant reagent from the reaction chamber Remove the F1 and F2 bottles from the cabinet and select UNLOADED e Dispose of the waste reagent in accordance with local procedures and regulations e Start another flush using the water in F3 e Remove the Fix 1 and Fix 2 bottles from the cabinet and select UNLOADED e Dispose of waste reagents in accordance with local procedures and regulations e The unload procedure will continue by highlighting the current dehydrant bottle and the Ex 1 position bottle 60 Shandon Excelsior ES Operator Guide Follow the screen instructions to confirm that the bottle Fx1 is empty Press UNLOAD to continue to remove the reagents Remove the Ex 1 bottle and dispose of in accordance with local procedures and regulations Place another empty bottle in the Fx 1 position and follow the screen instructions to remove the remaining dehydrants When the last dehydrant has been unloaded the screen returns to the Instrument Setup menu Shandon Excelsior ES Operator Guide 61 62 Shandon Excelsior ES Operator Guide Introduction Processing in Menu Mode Chapter 4 Menu Mode The Menu Mode sc
64. lide the metal cover up and remove it Slide the filter up and remove the plastic wrappers 2 Replace the filter ensuring that the airflow arrow points away from the instrument 3 Replace the metal filter cover locate the bottom of the cover behind the filter and then push the filter cover down and towards the front of the instrument The cover should be securely positioned on the raised wall M SS ASS SS EG I SS The optional vent adaptors allow fumes to be extracted into a fume cupboard or hood or vented to the outside atmosphere DO NOT USE THE VENT ADAPTORS TO EXTRACT THE FUMES THROUGH THE BUILDING HVAC SYSTEM OR A COMMON SITE EXTRACTION SYSTEM A Shandon Excelsior ES Operator Guide 89 Extraction Vent Adaptor Downdraft Extraction 90 Vent Adaptor Connecting to Mains Power Fit the vent adaptor to the instrument rear with the fixings supplied Route the ducting to a vented area Do not remove the main extraction filters Remove the downdraft filter and the metal filter cover Fit the downdraft extraction bracket into the filter pocket and press downwards and forward until it is secure Route the ducting to a vented area Ensure that the mains supply voltage corresponds with the voltage rating on the rating plate on the back of the instrument Note 1 The symbol on the rating plate indicates that the instrument operates on an alternating current supply AC Ensure th
65. nts clearants and infiltrants e Excelsior ES discards the used reagent from the appropriate containers used for the first steps in these processing groups Excelsior ES rotates the rest of the reagents Excelsior ES uses the fresh reagents in the last step for those process groups which have been rotated Shandon Excelsior ES Operator Guide 31 Reagent Rotation An example procedure is described here the feature Control All Example 1 Groups in the Quality Control screen enables alcohol in A1 xylene in X1 and wax in W 1 to be replaced together The timeline assumes a schedule of one process run per day DAY 1 The alcohol guality has reached its defined limit and a triangle is displayed above the alcohol guality indicator The Ouality Control screen is displayed automatically when the next processing run is due to begin 1 In the Quality Control screen highlight the text Control All Groups The text below alcohol and xylene is Rotation Due next Run The text below wax is Discard Due next Run The selection key options are ACKNOWLEDGE ALL and POSTPONE ALI J 2 Select ACKNOWLEDGE ALL 3 Open the doors to ensure that containers Ex1 Ex2 and WW are empty waste wax tray WW should have a liner 4 Run your next process to continue with the rotation Excelsior ES uses reagents as usual from Al X1 and WI discarding them into the exchange positions and the waste wax tray After the process steps u
66. o Cheshire WA7 1PR UK MI 49008 USA Tel 44 0 1928 562600 Tel 1 800 522 7270 Fax 44 0 1928 562627 Fax 1 269 372 2674 www thermo com pathology www thermo com pathology The Shandon Excelsior ES meets the following CE Mark requirements C In Vitro Diagnostic Directive 98 79 EC SP Low Voltage Directive 73 23 EEC as amended by 93 68 EEC e US Shandon Excelsior ES Operator Guide Contents How to Use This Guide 7 Safety Information 8 1 Introducing Excelsior ES 13 Overview Excelsior ES Instrument Tour Systems Monitoring How You Process With Excelsior ES About the Excelsior ES Interface Menus and Options 2 Daily Operations Mode 23 Introduction Option Keys Loading Specimens Level Key Starting the Current Process Starting a Different Process Removing Specimens The Ouality Control Screen Replacing Reagents Reagent Rotation Daily Options Menu 3 Instrument Setup Menu 39 Introduction Set Clock Define Reagent Groups Load Reagent Groups Colour Coded Reagent Tubes Edit Program Changing Program Title Defining Programs Program Step Parameters Customisation Process Start Selection Options Rotation Management Concept Demonstration Daily Operations Unload Reagent Groups Daily Options Menu 4 Menu Mode 63 Introduction Set Clock Processing in Menu Mode Loading Specimens Starting the Current Process Starting a Different Process Removing Specimens The Quality Control Screen Replacing Re
67. oratory Practice must be followed and ALL necessary precautions taken Where seismic regulations require the instrument to be secured use the handle locations at the rear of the instrument 2 x M8 female threads The Centre of Gravity position for a fully laden instrument is shown in the diagram Gravity Excelsior ES weighs approximately 112 kilograms 246 lbs when empty Do not try to move Excelsior on your own at least two people are needed to move an instrument Shandon Excelsior ES Operator Guide 10 If the instrument has been used with materials that are toxic or contaminated with pathogenic micro organisms follow the cleaning instructions given Before returning the instrument to Thermo the Product Return Certificate see Appendix D must be completed All reagents and filters used with Excelsior ES may become contaminated Personnel disposing of ALL reagents and filters used with Excelsior ES must follow local regulations for hazardous waste disposal The instrument should be regularly cleaned as described in Chapter 7 of this Operator Guide Use only factory approved accessories or replacement parts with Excelsior ES Correct maintenance procedures are essential for consistent performance It is recommended that a Maintenance Contract is taken out with your supplier The instrument must be serviced annually by a Thermo trained engineer in accordance with the instructions
68. ould comply with the reguirements of IEC1010 1 and or IEC950 Set LIMS Excelsior ES can send messages via the serial Interface D connector to report events listed on the LIMS screen Reagent and Wax Use this option to bring any concealed reagent or wax into the hecks reaction chamber outside of processing The Reagent and Wax Inspection Screen is shown 80 Shandon Excelsior ES Operator Guide Inspecting a Reagent A The screen shows e The loading dates for concealed bottles and wax baths The reagent usage count e The date of the last reagent rotation e The reagent usage count since the rotation RISK OF CONTAMINATION OF PROCESSING REAGENTS USE COMMON SENSE WHEN SELECTING WHICH REAGENT TO DRAW INTO THE REACTION CHAMBER Highlight the reagent bottle Select INSPECT REAGENT to move the reagent into the reaction chamber You can e Sample the reagent e Assess for correct volume the reagent level is just above sensor 3 when the instrument is loaded from 5L bottles If using 3 8 L 1 US Gallon bottles the chamber fluid level is just below sensor 3 e Add more reagent Select RETURN REAGENT to drain the chamber into the same bottle Or Shandon Excelsior ES Operator Guide 81 Select DISCARD REAGENT to discard the reagent Screen instructions prompt you to place an empty bottle in position Ex1 or EX Select CONFIRM EMPTY The reagent in the reaction chamber is drained into the empty bottle Disc
69. r of uses before alerts are displayed e Arrows indicating items which have reached limits Rotation information Rotation Discard due or Will Not Rotate Discard Position Reagents Count Usage Limit Filters Arrows If the Quality Control screen appears automatically when you start processing this means a usage limit has been reached For Fix Fix2 Fix3 F1 F2 F3 the limit is based on counts only The limits are set in the Quality Control screen but this can be done in Menu Mode only For filters the counts are displayed in weeks For Al X1 and WI the limit can be based on e Specific Gravity e Usage counts Day of the week Shandon Excelsior ES Operator Guide 29 Control All Groups Ouality Control and Yellow Triangle Alerts Replacing Fixative Reagents Fix1 2 The limit can only be changed from the Instrument Setup Rotation Management menu This is only available in Menu Mode see page 56 This refers to the rotation of dehydrants clearants and infiltrants If these reagents reach their usage limits at the same time the Control All Groups line is highlighted showing that this feature is available and you can rotate the three reagent groups together For fixative and flush reagents when the usage limit is reached a yellow triangle is displayed in the reagent bottle and at the top of the screen When the usage limit is reached for filters an alert triangle is displayed at the top
70. rain it immediately after entering the chamber From Menu Mode select Instrument Setup Rotation Management Highlight the Alcohol Ouality line Two selection keys Up and Down are displayed Shandon Excelsior ES Operator Guide 103 104 Shandon Excelsior ES Operator Guide Introduction A Chapter 7 Cleaning and Maintenance Normal standards of laboratory hygiene and routine maintenance procedures are all that is necessary to keep Shandon Excelsior ES in good and serviceable condition IF HAZARDOUS MATERIAL IS SPLIT ON OR INSIDE THE INSTRUMENT YOU MUST CARRY OUT THE APPROPRIATE DECONTAMINATION A CLEANING OR DECONTAMINATION METHODS OTHER THAN THOSE RECOMMENDED IN THE OPERATOR GUIDE SHOULD BE CHECKED WITH A THERMO AGENT TO ENSURE THAT THE PROPOSED METHOD WILL NOT DAMAGE THE EQUIPMENT A ALWAYS WEAR PROTECTIVE GLOVES WHEN YOU CLEAN OR DECONTAMINATE SHANDON EXCELSIOR ES TO PROTECT YOURSELF AGAINST THE EFFECTS OF CHEMICALS A DO NOT USE ANY CHEMICALS THAT MAY INTERACT WITH MATERIALS OF MANUFACTURE IF IN DOUBT CHECK WITH THERMO SERVICE DEPARTMENT A PHENOL AND HYPOCHLORITES IN STRONG SOLUTION WILL DAMAGE THE INSTRUMENT AND ITS ACCESSORIES A DO NOT USE ABRASIVE COMPOUNDS OR METAL COMPO NENTS TO CLEAN SHANDON EXCELSIOR ES OR ITS COMPO NENTS AND ACCESSORIES A ALWAYS WIPE UP ANY SPILLS IMMEDIATELY IN THE EVENT OF A MAJOR SPILLAGE DISCONNECT THE INSTRUMENT FROM THE MAINS SUPPLY WITHOUT DELAY AND DO NOT RECO
71. reen is shown below This chapter contains Processing in Menu Mode Using the Ouality Control screen Replacing fixative and flush reagents Reagent Rotation replacing wax and concealed reagents Options Reagent and Wax checks using the Inspect feature Processing in Menu Mode is the same as for Daily Operations with the exception that the LEVEL key option is permanently enabled Procedures are given below with reference to selection of the LEVEL key where appropriate Shandon Excelsior ES Operator Guide 63 Starting the 1 Open the reaction chamber push the knob and lift the lid to Current Process display the red status screen Instructions TANT t to begin the press PROCESS SELECT Process 2 Rapid Binpeler rocess End Time 01 47 huikea Processing end time Rapid Biopsies Current Program n n Inmediale Start Select to Select to start Select to same change bas prosessing end time kets dis played and fill level 2 Load baskets with a basket lid into the chamber stacked in uniform pairs and aligned so they sit properly on the agitation drive pins 3 Close the reaction chamber lid pushing down on both sides of the knob to ensure a good seal 4 Select LEVEL once for each pair of organised baskets up to three times for three pairs of baskets Select LEVEL four times for a random basket Ensure that the screen shows the baskets you put in the chamber If too many baskets
72. replace the filters follow the instructions in Chapter 5 Installing Excelsior ES Any reagent spills within the instrument will be contained Small spills such as drips from the reagent tubes when the reagents are changed will evaporate and be extracted To clean large spills remove all the reagent bottles in the Reagent Storage Area To access the spillage containment area lift and remove the metal shelf in the base of the Reagent Storage Area Clean any liquid from this area and dispose of in accordance with any local procedures or regulations Replace the metal shelf and the reagent bottles Shandon Excelsior ES Operator Guide 111 112 Shandon Excelsior ES Operator Guide Specification Physical Electrical Interface Connections Appendix A Specification and Accessories Width 670 mm 26 ins Depth 520 mm 20 ins Height to monitor platform 1130 mm 441 ins Height to top of monitor 1600 mm 63 ins Weight with no reagents 112 kg 2461 lbs Weight with typical reagents 200 kg 440 Ibs Power Supply Voltages 100 240 VAC Frequency 50 60 Hz Power 950 VA maximum 200VA typical Maximum supply voltage fluctuations not to exceed 10 of nominal voltage Internal batteries Yuasa NP 12 12 12V 12Ah valve regulated sealed lead acid type rechargeable battery Remote Alarm 3A 240 VAC operation non powered output LIMS Serial RS232 Netmon Serial RS232 Printer Centronics Parallel Shandon
73. rol screen is displayed automatically when the next processing run is due to begin It shows that alcohol and wax have reached the usage limit but xylene has not In the Quality Control screen The text below alcohol is Rotation Due next Run The text below xylene is Will not Rotate next Run The text below wax is Discard Due next Run 1 a Highlight alcohol and select ACKNOWLEDGE b Highlight wax and select ACKNOWLEDGE 2 Open the doors to ensure that containers Ex and WW are empty waste wax tray WW should have a liner 3 Run your next process Excelsior ES uses reagents as usual from Al and W1 discarding them into the exchange positions and the waste wax tray After the process steps using reagents A2 and W2 these are not returned to these positions they are moved forward by one position and become the new reagents for Al and W1 This continues for all subsequent reagents DAY 2 The next morning the screen indicates A6 and W3 are empty whilst Ex 1 and WW are full as shown 74 Shandon Excelsior ES Operator Guide Ld us nm i 1 0 Empty exchange containers 1 Replace the wax a Open the cabinet doors and take out the waste wax tray WW The Ouality Control screen appears b Take the second waste wax tray WW supplied with the instrument and put it with a new liner back in the cabinet In the Ouality Control screen highlight WW and select Confirm Empty c Add par
74. rows indicated items have reached limits e Rotation information Rotation Discard due or Will Not Rotate Discard Position Reagents Count Usage Limit Filters Arrows For Fix1 Fix2 Fix3 F1 F2 F3 the limit is based on counts only The limits are set in the Quality Control screen when in Menu Mode For filters the counts are displayed in weeks For Al X1 and W1 the limit can be based on e Specific Gravity e Usage counts Shandon Excelsior ES Operator Guide 69 Control All Groups Ouality Control and Yellow Triangle Alerts Replacing Fixative Reagents Fix1 2 Day of the week The limit is set from Instrument Setup Rotation Management see page 56 This refers to the rotation of dehydrants clearants and infiltrants If these reagents reach their usage limits at the same time the Control All Groups line is highlighted showing that this feature is available and you can rotate the three reagent groups together For fixative and flush reagents when the usage limit is reached a yellow triangle is displayed in the reagent bottle and at the top of the screen When the usage limit is reached for filters an alert triangle is ma at the top of the screen only For dehydrants clearants and infiltrants wax the Ouality Control screen is displayed automatically at the beginning of a process run If alcohol quality at bottle A1 is set as the trigger for rotation a triangle is displayed above
75. s e Take the second waste wax tray WW supplied with the instrument and put it with a new liner back in the cabinet In the Quality Control screen highlight WW and select CONFIRM EMPTY e Add paraffin wax pellets to the empty wax bath W3 Highlight wax and select Start Heating e While the wax is heating go to 2 below 2 Replace alcohol e Take out container Ex1 waste alcohol and dispose of e Place fresh 100 96 alcohol into position Ex 1 In the Quality Control screen highlight the text under alcohol and select NEW 3 Before continuing ensure that the wax level is correct e Running your next process completes the reagent rotation Shandon Excelsior ES Operator Guide 35 Daily Options Menu Download From the Daily Operations screen select OPTIONS to display the Daily Options screen below i N MI E a Instructions A eaae ane nins m Gunvcueus Select SET CLOCK to display the setting options if necessary Select MENU MODE to move to Main Menu You can download instrument technical files to a 3 5 inch floppy disc Before inserting it into the floppy disc drive ensure that the disc complies with the following e Formatted for IBM PC Ensured to be virus free with an up to date virus checker Contains no data e Not write protected ALL of the above must be satisfied for the download to be successful If you are unclear about any of these details consult your I
76. s fuse Replace have blown the instrument fuses Note Only a technically compe tent person should replace fuses Excelsior ES is Instrument in IS N powered ON but Standby screensaver Press Oon ELN the screen is blank ON instrument out of Standby The monitor is not At the back of the instrument connected correctly ensure that the power and signal cables are correctly connected The monitor is Replace the monitor Contact faulty your Thermo dealer A process is The lid is not Open the lid and close by started but the closed pressing down firmly on either first reagent is snot side of the knob do not use the drawn in knob itself to close the lid The lid seal is con Clean the seal lid taminated with wax After loading The second short Return to the Load Reagents reagents a Process flush routine at menu and complete the se Start screen isnot the end of the load quence displayed sequence has not been run Shandon Excelsior ES Operator Guide 97 98 The process start screen is not displayed and a message reguests reagent transfer The OWERTY keyboard is dis played at the start of a run An underfill is registered on first transfer but for no obvious reason An underfill warn ing occurs for the front reagents but the bottle is full Bottles AG and X3 appear empty The waste wax tray is not registered as fitted and a warning triangle is displayed Th
77. sing reagents A2 X2 and W2 these are not returned to these positions they are moved forward by one position and become the new reagents for Al X1 and WI This continues for all subsequent reagents DAY 2 The next morning the screen indicates A6 X3 and W3 are empty whilst Ex1 Ex2 and WW are full as shown 32 Shandon Excelsior ES Operator Guide Replace the wax Open the cabinet doors and take out the waste wax tray WW The Quality Control screen appears Take the second waste wax tray WW supplied with the instrument and put it with a new liner back in the cabinet In the Quality Control screen highlight WW and select CONFIRM EMPTY Add paraffin wax pellets to the empty wax bath W3 Highlight wax and select START HEATING While the wax is heating go to 2 below Replace alcohol and xylene Take out containers Ex1 waste alcohol and Ex2 waste xylene and dispose of them Place fresh 100 alcohol and fresh xylene into positions Ex1 and Ex2 In the Quality Control screen highlight the text under alcohol and select NEW Highlight the text under xylene and select NEW Transfer reagents to concealed bottles Before continuing ensure that the wax level is correct Running your next process completes the reagent rotation Shandon Excelsior ES Operator Guide 33 Reagent Rotation Examples of reagent rotation procedures are described here for Example2 rotating reagents as directed by the Qualit
78. the alcohol guality gauge When you see the yellow warning triangle alert inside fixative and flush reagent bottles you should change the reagents before running the next process Open the lower doors the Quality Control screen appears Then 1 Remove the reagents and tell Excelsior ES a Take out the Fix and Fix2 bottles and discard them b Highlight Fix and select REMOVE 70 Shandon Excelsior ES Operator Guide Replacing Fixative Reagents Fix1 3 Reagent Rotation Overview c Highlight Fix2 and select REMOVE 2 Place the new reagents and tell Excelsior ES a Put new bottles of fixative in positions Fix1 and Fix2 b Highlight Fix2 and select NEW c Highlight Fix1 and select NEW This zeroes the Ouality Control counter and removes the warning triangles from the screen Open the lower doors and remove flush reagents in the order F2 F1 F3 In the Quality Control screen 1 Highlight F2 and select REMOVE 2 Repeat for F1 and F3 3 Place the new reagents in positions F3 F1 F2 In the Quality Control screen highlight each reagent in turn and select NEW Excelsior ES rotation is a unigue in process system for maintaining dehydrants clearants and infiltrants e Excelsior ES discards the used reagent from the appropriate containers used for the first steps in these processing groups Excelsior ES rotates the rest of the reagents Excelsior ES uses the fresh reagents in the l
79. tion Routine Overnight Program Rapid Biopsy Program Appendix C Program Examples These are the default programs installed on Excelsior ES Leser e pue Jo acu v pe cm jo gt pe pm meet spem o K a sp s que Eoque op S L JN N KN KN woe p peitot t npe p pep p F ep p p e e Ja roose Tm foan wu sr E 1 C gt _ ia sm o Jo Goon gt _ a ea 9 Jo Goon 3 is user o o on gt is user Juo o on wa eea Era ena aa EnS e C I Inm C B fe us Inm Ja fon id s ee 8 e 1 jap vn 5 Shandon Excelsior ES Operator Guide 121 Standard Flush tron Extended Flush REAGENT HOLD USE TIME DRAIN VACUUM STIR Program Rinse Program f Trg vowruse me Oman fcon sw 122 Shandon Excelsior ES Operator Guide Introduction Packaging the Instrument Appendix D Transportation Instructions If the instrument is to be transported follow these packaging instructions after unloading ALL reagents and wax e Use ALL original packaging e Keep the instrument upright at ALL times 1 Place the base of the packaging in a clear area 3m is required in front of the packaging for the ramp and approximately 1m is required behind the packag ing Lower the ramp of the packaging 3m dd 2 Fit the handles to the back of the instrument WHEN MOVING EXCELSIOR ES USE SAFE LIFIIN
80. tle List Empty Program Positions Highlighted Title in Bold Text Notes Field From this screen you can e Change Program titles e Produce all new user defined Program titles e Add Notes 1 Highlight the title you want to change 2 Select CHANGE TITLE to display a column with pre defined and user defined titles 3 Move down the list to highlight any slot then select SELECT Highlight a user defined slot in the program title list Select CHANGE TITLE to display the OWERTY keyboard 3 Follow the procedure on page 43 to enter the new title ex and press EXIT to enter the Define Program screen Ne Shandon Excelsior ES Operator Guide 51 Adding Notes From the Program Select screen select CHANGE NOTES the QWERTY keyboard is displayed Using the text entry procedure see page 43 enter the text to appear whenever the Program Select screen for that title is displayed Select NEW NOTES EACH RUN to prompt the operator to add notes at the start of every process run which uses the highlighted program Note If this is selected the QWERTY keyboard is displayed before the start of every process run and must be cleared before processing can start A Select FIXED NOTES to display the same text in the Notes field Defining Highlight a program title and choose SELECT to display the Programs Define Program screen with program steps and parameters REAGENT HOLD USE TIME DRAIN VACUUM ST
81. to a printer e To the Excelsior ES screen e To the Excelsior ES disc drive 78 Shandon Excelsior ES Operator Guide Disc Drive Options Audio Remote Alarms 1 Highlight the information you want to print 2 Select a destination from the key options Electrical connections for a printer are detailed in chapter 5 Installing Excelsior ES The Disc Drive Options screen is shown 1 Highlight an option in From the list 2 Insert a loppy disc into the disc drive 3 Select SELECT Excelsior ES can save programs and instrument setup information on standard 3 5 floppy discs New languages and features can also be loaded The Audio Remote Alarms screen is shown with default settings Shandon Excelsior ES Operator Guide 79 The screen shows events monitored by Excelsior ES which can trigger alarms alerting operators to act and ensure processing guality To change settings 1 Highlight an event 2 Select from the sounding options Use the number keys for repeated sounds or CONTINUOUS to sound the alarm until it is silenced with the UE button on the keypad 3 For the remote alarm highlight under RAI and RA2 and select either OFF or ON Different tunes can be selected to play for each event Both remote alarms can be selected and operated for any combination of events External circuits should only be connected to the Remote Alarm socket by a technically competent person The external circuit sh
82. trument for fixative storage Dehydrants are stored in six concealed bottles at positions Al A6 in the back of the cabinet The bottles are loaded with solutions of different concentrations e The bottles are permanently fixed and not accessible to operators You work with one exchange bottle at position Shandon Excelsior ES Operator Guide 21 22 Shandon Excelsior ES Operator Guide Introduction Daily lela ale verview Selection Keys Described Chapter 2 Daily Operations Mode From Main Menu select 8 Daily Operations to display the Daily Operations screen below mu A LIT dha h h h ha a Routine processing flushing and reagent management are available no other menus are needed When you open the reaction chamber lid the red status screen shows the current processing program e After processing is complete Excelsior ES automatically shows the flush program to clean the chamber When reagents or filters are depleted Excelsior ES informs you which items need to be replaced PROCESS displays a red status screen with a current process program FLUSH displays a red status screen with a current flush program Use this to run separate flush and rinse cycles outside of routine processing as may be required OPTIONS displays the Daily options screen described at the end of this chapter QUALITY CONTROL displays the Quality Control screen Use this for reagent management Shandon Excelsior
83. ush cycle is finished the screen displays the Instrument Setup menu and Excelsior ES is ready for use Ensure that you leave an empty bottle in position Ex2 with the RED tube inserted Leave an empty bottle in position Ex1 with the BLUE tube inserted Ensure that you put the caps over the necks of the bottles to prevent reagent evaporation Notes 1 Thermo recommend that you load the reagents in this order so Excelsior ES is fully flushed with the correct reagents 2 Once the instrument is fully loaded the Load Reagents Menu cannot be accessed again A Loading Fixatives in positions Fix and Fix2 are not loaded from the Load FixatiVeS Reagents menu Instead the Quality Control screen is displayed before the first process run It will prompt for fixatives to be loaded and NEW to be selected for Fix and Fix2 if Fix2 is to be used When this happens 1 Put fixative bottles in positions Fix and Fix2 inserting the GREEN reagent tubes fully together with GREEN caps 2 Select NEW 3 Exit the Quality Control screen 50 Shandon Excelsior ES Operator Guide 4 Edit Program Changing a Program Title Producing a User defined Program Title The Program Select screen is displayed below showing Current program titles Program duration times based on the individual step times e A Notes field where further information can be entered for display when programs are highlighted Program Ti
84. vel When the instrument is level the water settles evenly at the bottom of the chamber If necessary adjust the front castors Loosen the locknut a wrench is provided then turn the castor with the adjustment rod provided When the instrument is level tighten the locknut Mop up the water and dry the reaction chamber 86 Shandon Excelsior ES Operator Guide Connecting a Printer Connecting a Remote Alarm 1 Remove the monitor and base from the packing 2 Secure the monitor base to the monitor instruction sheet in accessories box Place the monitor assembly on top of the instrument Connect the power and signal cables between the monitor and the instrument Secure the D type signal connector by tightening the screws K Go THE MONITOR USES 12V DC VOLTAGE ONLY DO NOT PLUG THE POWER LEAD INTO ANY OTHER POWER SUPPLY A ALL ACCESSORIES MUST BE CONNECTED TO EXCELSIOR ES BY TECHNICALLY QUALIFIED PERSONS ONLY A Mains P ower Power ON OFF Mains Fuses Remote alarm fuses I Monitor Signal EI Remote Alarm 1 Remote Alarm 2 Printer a Printers can be connected using the parallel printer port shown on the electrical connections panel Printers used should comply with IEC950 or IEC1010 1 Connections for Remote Alarms 1
85. y Control screen Control All Groups The timeline assumes a schedule of one process run per day DAY 1 The alcohol guality has reached its defined limit and a triangle is displayed above the alcohol guality indicator The Ouality Control screen is displayed automatically when the next processing run is due to begin It shows that alcohol and wax have reached the usage limit but xylene has not In the Ouality Control screen The text below alcohol is Rotation Due next Run The text below xylene is Will not Rotate next Run The text below wax is Discard Due next Run 1 Highlight alcohol and select ACKNOWLEDGE Highlight wax and select ACKNOWLEDGE 3 Open the doors to ensure that containers Ex1 and WW are empty waste wax tray WW should have a liner 4 Run your next process Excelsior ES uses reagents as usual from Al and W1 discarding them into the exchange positions and the waste wax tray After the process steps using reagents A2 and W2 these are not returned to these positions they are moved forward by one position and become the new reagents for Al and W1 This continues for all subsequent reagents DAY 2 The next morning the screen indicates A6 and W3 are empty whilst Ex1 and WW are full as shown 34 Shandon Excelsior ES Operator Guide 1 Replace the wax uu m m aL Open the cabinet doors and take out the waste wax tray WW The Quality Control screen appear
86. ys Operator Guide Screen Information Cards Service Manual Spatula 20 1 set 1 set PART NUMBER A78410025 A78410021 A78420158 A78420156 B35210007 A78410023 A78410024 9990610 9990612 Pack of 6B9990612CS A78410029 A78410026 A78410027 AP 14747 AP 14733 A78410100 A784 1002 A78410101 P09046 Shandon Excelsior ES Operator Guide 115 116 Reagents Wax fill chute FORMAL FIXX Concentrate 5x 3 8 litre bottle makes 19 litres Concentrate 5x 1 litre bottle makes 5 litres Ready to use 19 litres 5 gal Pre filled cups see catalogue for full details Wrench for 19 litre carboy Zinc Formal Fixx Concentrate 5x 3 8 litre bottle makes 19 litres Ready to use 19 litres 5 gal Wrench for 19 litre carboy GLYO FIXX Ready to use 3 8 litres Ready to use 19 litres 5 gal Concentrate 5x 3 8 litre bottle makes 19 litres Pre filled cups see catalogue for full details Wrench for 19 litre carboy SHANDON XYLENE SUBSTITUTE Ready to use 4 litres Ready to use 4 litres Ready to use 10 litres Ready to use 19 litres 5 gal Ready to use 209 litres 55 gal Wrench for 19 litre carboy PARAFFIN WAX Histoplast 10 kg bag Histoplast 2 5 kg box Paraplast 1 kg bag Paraplast Plus 1 kg bag Shandon Excelsior ES Operator Guide 1 1 1 1 1 e m N A coo CO A78430265 QUANTITY PART NUMBER 6764254 9990244 6764

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