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1. 39 5 4 7 Setting the section thickness ssssssssesesrsrerersrursrerurururunurururururunnnnnnurnnnnunnnntananannnnnnnnnnnnnanunnnnnnnnnae 39 5 5 Daily operation of the instrument sesesronrenvnnerverrenvnververrervenvsverversenrsserverrersesesverversessssesvenversenenne 40 5 5 1 Freezing specimens onto specimen discs with quick freeze shelf and stationary heat extractor cccceececscsscsscssssscssseccecsessuseeseesesseseesecsesenseacesseseesausassesoesausaasecesensaneeees 40 5 5 2 Freezing specimens onto specimen discs with 90 prism Instruments W SPECIMEN cooling Only i evenvrererverenerrererverenerrerervereserreneresreservevesesvesesvenesenvene 41 5 5 3 Optional accessories for freezing specimens onto specimen discs and for removing frozen SDE CINE HS Lue 41 5 5 4 Inserting specimen discs into the SPECIMEN head memmesvrververerveverververrenrrnerverresreververserseseesenne 42 5 5 5 Specimen TENG 42 annn ee ONN pea aaa E A eteuianncnen naan 43 6o AUIS FUNG NOTK EE 44 DI TEN 44 5 6 2 Switching off the instrument for a longer period Of time srsrernanrnnvnverrervenvaververrenrenervervenvenenne 44 EE EE NE EE 45 5 7 1 Automatic chamber defrost cycle aiicceiesiatiessandstavsasdsavinvinvenitaieatvisamndsicvenivsaveavtdstadsdsldncarvauveadaiaciine 45 5 7 2 Manual chamber defrost cycle sssssssssssssesssesurenurererururununurunurununnnnnnnururunntntntanunnnnnnnnnnunnnununnnnnnnnnnnae 46 5 7 3 Defrosting
2. Chapter 2 3 5 e Motorized sectioning Chapter 2 3 6 e Defrosting handling frozen tissue Chapter 2 3 7 e Frozen instrument parts and acces sories Chapter 2 3 8 e Infectious radioactive material Chapter 2 3 9 5 5 1 Freezing specimens onto specimen discs with quick freeze shelf and stationary heat extractor Size the specimen Apply sufficient amount of embedding medi um onto ambient temperature specimen disc Inside the cryochamber place the specimen onto the disc and orient Place specimen disc with specimen into one of the 10 openings in the quick freeze shelf see below and freeze The freezing process can be sped up further by using the stationary heat extractor 1 Next to the low temperature stabilizer 2 Parking Station 3 specimen discs 3 can be placed within Its pivoting range Place the heat extractor 1 directly onto the specimen surface After approx 30 seconds the specimen Is completely frozen Instruction Manual V1 3 03 2008 5 Operation 5 5 2 Freezing specimens onto specimen discs with 90 prism Instruments w specimen cooling only In instruments with specimen cooling the 90 prism which ts inserted into the specimen head is an additional option to freeze specimens at maximum temperature Installing the 90 prism e Loosen screw 3 at specimen head e Insert pin 1 of prism completely into recep tacle
3. Chapter 2 3 7 ber and empty e Frozen instrument parts and e Remove remaining section waste from cryo accessories chamber Chapter 2 3 8 e Working accessories brushes specimen Infectious radioactiv material holders etc can be disinfected inside the Chapter 2 3 9 cryochamber e Once the selected chamber temperature has been reached spray Leica Cryofect on the surfaces and accessories to be disinfected e Allow to soak for 15 minutes e Use paper towels or another type of disposable cleaning cloths to wipe up disinfectant and remaining section waste e Dispose of paper towels together with sec tion waste e f previously altered set chamber tempera ture back to its former value e Putspecimens back into the cryochamber Leica CM 30508 Cryostat n3 1 Disinfection cleaning and maintenance 1 3 i 54 Disinfection with conventional disinfectants Switch the instrument off Carefully remove knife blade as well as spec imens and accessories from the chamber Remove section waste from the chamber and dispose of it Remove sliding window see chapter 7 6 Ex changing the fluorescent light lamp Remove microtome see chapter Removing the microtome Allow the microtome to thaw disinfect and allow to dry thoroughly 7 9 1 Drying the microtome e Use a hairdryer dry and before reinstalling into the chamber allow the microtome to cool down to room
4. e Switch off mains switch e Unplug the instrument from mains e fthe lamp is broken or splintered Have lamp replaced by Leica Technical Service gt Risk of injury Use only those replacement lamps that cor respond to technical specification see chapter 4 2 Technical Data 15 3 Installation 3 1 Site requirements Make sure to read and follow all safety instructions in chapter 2 3 2 Site requirements A 3 1 1 General site requirements e No direct sunlight e Electrical power supply within distance length of power cord approx 4 meters do not use extension cords e No draft caused by air conditioning etc e Even floor surface e Practically vibration free floor Handwheel easily accessible Room temperature constantly below 22 C Relative humidity of air maximum 60 High ambient temperature and or high i air humidity negatively affect instrument cooling performance 3 1 2 Electrical connections Make sure to read and follow all safety instructions in chapter 2 3 3 Electrical connections A 16 3 2 Unpacking and installation Unpacking instructions are always located in a transparent protective envelope on the outside of the instrument shipping crate A 3 2 1 Repacking Make sure to read and follow all safety instructions provided in chapter 2 3 1 Transport and on the unpacking instructions We
5. C Instruction Manual Leica CM 3050 S Cryostat English V1 3 03 2008 Always keep this manual near the instrument Read carefully prior to operating the instrument Leica CM3050 S Cryostat Cca MICROSYSTEMS Important note The information contained in this document represents state of the art technology as well as Year of Manufacture sessies the current state of knowledge Leica will notassume liability for errors that might be contained in this manual nor for accidental damage or damage arising from the delivery performance or use of this manual Country of Origin Federal Republic of Germany Therefore no claims can be made based on the text and illustrations in this instruction manual Leica Biosystems Nussloch GmbH reserves the right to change technical specifications without notice since each of our products Is subject to a policy of continuous improvement This document is protected under copyright laws Leica Biosystems Nussloch GmbH retains all rights related to this documentation Any repro duction of text and illustrations or any parts thereof in form of printing photocopying micro fiches or other methods including electronic sys tems requires the prior written permission of Leica Biosystems Nussloch GmbH The serial number and the year of manufacture are specified on the nameplate at the back of the instrument Jeica Leica Biosystems Nussloch GmbH MICROSYS
6. Switching specimen cooling back on immedi ately after defrosting As soon as the chamber defrost cycle Is com pleted specimen cooling can be switched back on immediately e Press until menu item Set specimen temperature is displayed SJEIT TIEIMIP Jo T 3 5 c TETT TTTTTTTT TT lal e Press CH SJE T TJEIM P O T 3 5 C RRR EERE Specimen cooling is switched back on Despite the possibility of switching the specimen cooling system back on im mediately after the automatic chamber defrost cycle we recommend the auto matic defrost cycle be set at a point in time when the entire procedure can be controlled automatically See also information on menu items 6 and 7 in chapter 5 3 4 Menu func tions i 45 5 Operation 5 7 2 Manual chamber defrost cycle In addition to the automatic defrost cycle a manual chamber defrost cycle can be activat ed when necessary e Press until menu item Set chamber temperature is displayed SJEIT TEMP Jc T 3jo c TT TTTTTTTT TT fe e Press EA A brief sound signals that the manual chamber defrost cycle has started SJEIT TIEIMIP C T 3lo c The manual defrost cycle ends either when the selected defrost cycle duration is over or when a chamber temperature of 5 C is reached To abort the manual defrost cycle e Go back to menu item Set chamber tem perature and press S again Instrum
7. wards the knife as long as the key Is held Coarse feed slow forward Travelling speed 500 um s e To activate the function press and hold The specimen head travels forward towards the knife as long as the key is held STOP LED located in key W Coarse feed fast forward As long as the forward coarse feed both fast and slow is in motion the STOP LED is illumi nated Upon reaching the front limit position the STOP LED starts flashing and the display in control panel 1 reads STOP Instruction Manual V1 3 03 2008 5 Operation Functional area B all models 5 4 6 Trimming and sectioning functions switching between trimming and sectioning mm Press this key to alternate between the ST f two functions tam illuminated Trimming mode is active trm Off Sectioning mode Is active Selecting trimming thickness e Activate trimming mode e Select trimming thickness sectioning thickness at which trimming Is carried out by pressing or The selected thickness is displayed in the cor responding LED 5 70 30 50 100 150 Motorized trimming e Select Continuous stroke in functional area A e Activate trimming by pressing RUN STOP and RUN ENABLE located in functional area C simultaneously or by depressing the footswitch e To stop trimming press RUN STOP or RUN ENABLE or depress the footswitch again Trimming thickness can b
8. Retraction stroke Sectioning stroke inside sectioning window Sectioning UU stroke HL Sectioning window i Retraction stroke 32 Digits 3 6 indicate coarse feed position HOME STOP e Rear limit position e Front limit position indication of malfunction or handwheel locked e Microtome plug loose implausible values etc LOCKED Indication LOCKED completely overlays digits 3 through 16 i e while LOCKED is displayed no other parameters will be displayed in digits 3 16 of the lower line e Handwheel is locked e Further indications see chapter 6 1 Error messages indicated in the display screen Instruments with chamber cooling only FIT eit psoe tt mjajHfojME I IT Pe Mee OTD9G0000000000 aie Sie 1 2 ft lo eles See tee Digits 7 16 indicate the following parameters e Refrigeration status Digit 15 cryochamber Digit 16 specimen Meaning of l refrigeration Refrigeration OFF symbols Refrigeration ON Ey Defrosting active e Section thickness set value e Section counter actual value e Indication of total section thickness actual value e Preset counter Digits 7 16 Scroll via arrow buttons to display individual parameters Instruction Manual V1 3 03 2008 5 Operation 5 3 6 Indication of refrigeration state section thickness setting and counters These parameters can be displayed
9. and correctly aligned anti roll plate 48 Edge of anti roll plate dam aged Blunt knife Knife and or anti roll plate dirty Clean with dry cloth or brush Replace anti roll plate Use different part of knife or replace knife Instruction Manual V1 3 03 2008 6 Troubleshooting applications tips Problem Causes Remedies Sections curl on anti roll plate Anti roll plate does not pro trude far enough beyond the knife edge Readjust correctly Scraping noise during section ing stroke and specimen return stroke Anti roll plate protrudes too far beyond the knife edge and is scraping against the specimen Readjust correctly Ondulated sections Knife damaged Edge of anti roll plate damaged Use different part of the knife or replace knife Replace anti roll plate Chatter marks form during sectioning Specimen insufficiently frozen onto specimen disc Specimen disc not clamped tightly enough Specimen holder ball joint not clamped Knife not clamped tightly enough Selected section thickness too thick specimen has come off the specimen disc Specimen very hard and inho mogeneous Blunt knife Knife profile inappropriate for the type of specimen to be sectioned Wrong clearance angle Refreeze specimen onto disc Check disc clamping Check ball joint clamping Check knife clamping Refreeze specimen onto disc Increase section thickne
10. temperature e Allow to dry at room temperature at least over night longer if nec essary Disinfect and dry disassembled parts accessories and tools Disinfect chamber walls with commercial alcohol disinfectant Place an adequate container underneath the drain tube at the right side of the instrument Pull stopper out of chamber bottom and drain off disinfecting solution into the con tainer Put stopper back into drain in cryochamber Allow cryochamber to dry thoroughly Reinstall microtome see chapter 7 5 3 Only when they are completely dry reinsert the disinfected accessories tools into the cryochamber Reinsert the sliding window Switch the instrument back on Once a suitable chamber temperature has been reached return the specimens to the cryochamber 7 4 Cleaning When necessary the instrument surfaces can be cleaned with mild commercial house hold cleaners Surfaces which are potentially contaminated infectious germs should always be cleaned with alcohol disinfectants Instruction Manual V1 3 03 2008 7 Disinfection cleaning and maintenance 7 5 Removing reinstalling the 7 5 2 Disassembling the specimen head microtome instruments w specimen cooling only 7 5 1 Removing the microtome e Use Allen key size 5 to remove screw 2 from the center of the specimen head 1 sure to observe the following safety in structions e Removing reinstalling the micro
11. tome Chapter 2 3 11 AN When removing the microtome make e Defrosting handling frozen tissue Chapter 2 3 7 e Infectious radioactive material Chapter 2 3 9 e Do not twist the refrigerating tube see chapter 2 3 11 Removing reinstalling the microtome e Hold hook 4 part of standard delivery in one of your hands and with your other hand rest refrigerating tube 3 on hook Insert hook 4 with refrigerating tube 3 into the opening above mark V e Disinfection and cleaning Chapter 2 3 10 e Prior to removing the microtome carry out preparation steps as per chapter 7 3 switch instrument off remove accessories etc Leica CM 30508 Cryostat 55 7 Disinfection cleaning and maintenance Remove plug of temperature sensor 7 Remove plug of coarse feed motor Pull bushing backward arrow 1 and hold Place your thumb between plug 6 and socket at the microtome arrow 2 To remove plug 6 from microtome pull it away from the microtome while at the same time carrying out a slight jogging movement arrows 3 56 Loosen screws 8 with Allen key Lift microtome a little and pull slightly to the left This loosens the plastic coupling 11 which connects the two axles Remove the microtome from the chamber 7 5 3 Reinstalling the microtome make sure to observe the following safety instructions When reinstall
12. 1 and 6 2 These chapters list the most common types of problems which many times have simple causes that can be eliminated by the actual user If you find Call Technical Service in the Remedy column please do so Never try to solve these problems yourself 25 5 Operation 52 Switching on fuses ou GJ TO TJA 2 Automatic cutout for sectioning motor 1 Combination Mains switch automatic cutout for the instrument Switching on e To switch on the instrument place switch 1 in upper lock in position Mains switch automatic cutout for the instru ment In addition to its function as ON OFF switch switch 1 is also the automatic cutout for the in strument If the automatic cutout has been triggered e g caused by overcurrent e Put switch 1 back in upper lock in position 26 Automatic cutout for sectioning motor The instrument is equipped with an automatic cutout 2 for the sectioning motor If the sectioning motor is overloaded e g when sectioning extremely hard specimens etc the automatic cutout 2 will be triggered To make the instrument functional again after the automatic cutout 2 has been triggered e Push automatic cutout 2 inwards until it locks into position Instruction Manual V1 3 03 2008 5 Operation 5 3 Control panel 1 i e Oo e a LI LEICA 30508 1 3 1 Key functions in control panel 1 fey ON OFF key for cryocha
13. 2 in the specimen head Important the entire rear surface of the prism must fit tightly against the specimen head surface e Retighten screw 3 at specimen head The prism now forms a horizontal surface for quick specimen freezing Freezing specimens with the 90 prism e Apply sufficient amount of embedding medi um onto ambient temperature specimen disc add specimen and orient e Insert specimen disc with specimen into the receptacle 4 of the prism e Press O to activate menu item Fast Object Freezing and activate function by pressing see chapter 5 3 4 Menu functions item 4 Leica CM 30508 Cryostat The specimen head is now automatically cooled down to the lowest temperature 50 C e Once the specimen is frozen remove the disc from the prism and store it in the quick freeze shelf until inserting it into the speci men head e Press again to activate menu Item Fast Object Freezing and deactivate the function by pressing once again see chapter 5 3 4 Menu functions item 4 The specimen head temperature now automati cally returns to the previously selected set val ue e Loosen screw 3 at specimen head and re move prism 1 e Store prism inside the cryochamber 5 5 3 Optional accessories for freezing specimens onto specimen discs and for removing frozen specimens Mobile heat extractor In addition to the stationary heat extractor part of standard delivery a mo
14. Ice Adapt speed Check knife clamping Check disc clamping Place embedding medium on luke warm specimen disc inside the cryochamber place the specimen onto the disc and freeze in quick freeze shelf Use different part of knife or replace knife Set correct clearance angle Prepare new specimen Tissue sticks to the anti roll plate Anti roll plate too warm or incorrectly adjusted Static electricity Fat on corner or edge of anti roll plate Knife rusty Cool or readjust anti roll plate Eliminate cause Remove fat alcohol Remove rust Flattened sections curl up as soon as anti roll plate is lifted from the knife 50 Static electricity or draft Anti roll plate too warm Eliminate cause Cool anti roll plate Instruction Manual V1 3 03 2008 6 Troubleshooting applications tips Problem Causes Remedies Sections tear Temperature too low for the tissue to be sectioned Knife blunt dirty dusty frosted or rusty Upper edge of anti roll plate damaged Hard particles in the tissue Rear face of knife dirty Select higher temperature and wait for the new temperature to be reached Eliminate cause gt see details on individual problems Exchange anti roll plate If application permits section at lower plane Clean Inconsistent or insufficient specimen feed Microtome was not completely dry when refrigeration was switched on ice bui
15. Type Marks of conformity Nominal voltage Nominal frequency Power draw Max start up current for 5 sec Protective fuse Automatic cutout Pollution degree Overvoltage installation category Heat emission max CM3050S 10 CM3050S 1 CM3050S 8 CM3050S 9 100 VAC 10 230VAC 10 240VAC 10 100 V AC 10 50 Hz 50 Hz 50 Hz 60 Hz 1800 VA 1800 VA 1800 VA 1800 VA 35 A eff 25 A eff 25 A eff 30 A eff T15A M3 T10A T1 T10A T1 T15A M3 2 2 2 2 1800 J s 1800 J s 1800 J s 1800 J s Refrigeration system Cryochamber Temperature selection range Defrosting Refrigeration capacity O Safety factor Refrigerant Compressor oil Quick freeze shelf Maximum temperature Number of quick freeze stations Specimen cooling Option Temperature range Defrosting Refrigeration capacity O Safety factor Refrigerant Compressor oil Leica CM 30508 Cryostat 50 Hz 0 C to 40 C 2K 0K Automatic hot gas defrost cycle programmable in 15 min increments 1 automatic defrost cycle per 24 hours Duration 6 12 minutes Manual defrost cycle 690 W 3 280 g 5g refrigerant R 404A 0 6 I EMKARATE RL 22S ICI 43 C 0 K 2K 10 10 C to 50 C 3K Manual defrost cycle electric 320 W 3 185 g 5g refrigerant R 404A 0 4 alpha 22 Kyodo or RENISO E22 Fuchs 60 Hz 0 C to 40 C 2 K 0K Automatic hot gas defrost cycle programmable in 15 min increments 1 a
16. by about 2 cm e Roll the cryostat onto the U frame as far as it will go The rear of the cryostat housing has to fit closely to the rear bar of the U frame ANN MAN ANS A e Roll the cryostat onto the U frame as far as it will go The rear of the cryostat housing has to fit closely to the rear bar of the U frame a Zz waa Zs ER A When placing the instrument onto the e Plug the height adjustment device into the ame AE se mre POE Cole mains socket The unit is now ready for use does not get caught and thus crushed l y l between U frame and cryostat hous ing 60 Instruction Manual V1 3 03 2008 9 Appendix 9 1 5 Control keys The control keys are located on top of the con trol unit see photograph To lift the instrument press 4 To lower the instrument press V As long as a button is held the instrument will go up or down until reaching the upper or lower limit 9 1 6 Maintenance and care Protect the lifting pistons in the four legs of the height adjustment device against dust and water Approximately every six months lubricate the legs with a thin coat of resin free oil Once every year have the height adjustment device checked by an authorized Leica ser vice engineer Leica CM 30508 Cryostat 9 1 7 Troubleshooting 1 Slight and uniform loss of height of the de vice In the course of several weeks IS nor mal Remedy Cor
17. e When released the grip locks into position Instruction Manual V1 3 03 2008 2 Safety 2 2 3 Emergency stop function instruments with sectioning motor only The emergency stop is activated via the red emergency stop button in control panel 2 or via the footswitch o Control panel 2 Footswitch Activating the emergency stop function e Press emergency stop button or step on footswitch forcefully gt STOP STOP red lights up As soon as the emergency stop function is acti vated the sectioning motor stops Deactivating the emergency stop e To deactivate rotate emergency stop button in direction of arrow If the emergency stop function has been activated by the footswitch unlocking is not necessary func tion is unlocked as soon as the footswitch is released i Leica CM3050 S Cryostat 2 2 4 Knife guard All knife holders are equipped with a knife guard gt see separate instruction manuals on knife holders A Always cover the cutting edge with the knife guard e Prior to doing any work on knife and or specimen e Prior to exchanging specimens e During work breaks 11 2 Safety 2 3 Safety instructions handling and operation of the instrument 2 3 1 Transport To avoid severe damage to the instrument by running It while the compressor oil is dis placed from its regular position e Do nottilt the i
18. i rinted circuit boards n O 2 electronic EET ee ache CARANA pep mechanical parts HEF Tete PE fpicalcomporents JPP O pes TO Oo iignsouces 2 o light sources o ATEGDEN SPT ERT NRS MST SJT 11363 2006 RER ENRE ERAF Indicates that the concentration of the hazardous substance in all materials In the parts is below the relevant threshold of the SJ T 11363 2006 standard x RABKABASMRAEYSERAFOR VBEPHSBRUSIT 11363 2006 IERTARE RR Indicates that the concentration of the hazardous substance of at least one of all materials In the parts is above the relevant threshold of the SJ T 11363 2006 standard Note The actual product may or may not include in all the part types listed above Leica CM 30508 Cryostat 67 Notes 68 Instruction Manual V1 3 03 2008
19. must be cleaned and decontami nated in the appropriate manner Since it Is not possible to decontaminate for prion diseases such as CJD BSE CWD etc equipment exposed to specimens containing prion diseases cannot be returned to Leica Microsystems for repair On site repair of prion contaminated equipment will only be conducted after the Field Service Engineer has been educated in the risks instructed in the policies and procedures of the institution and provided with personal protective equipment This certificate duly completed must be placed in the instrument attached to the outside of the shipping crate or handed directly to the service engineer Packages will not be opend nor servicing commenced until the Company or service engineer have received a satisfactory certificate Should returned goods be considered a hazard by the Company they will be re turned immediately to the customer at his her expense NB Microtome knives must be in boxes Description Name Model Fabr No KAT No Quantity A Yes This equipment has not been in contact with unfixed biological samples This equipment has been exposed internally or externally to hazardous materials as indicated below Please provide further detail here Blood body fluids pathological samples Other biohazards lt D 7 Been mL ILD Chemicals substances hazardous to health Other hazards This equipment has been cleaned and decontaminated Yes No If yes give de
20. recommend to keep the original shipping crate and the unpacking instructions for the Leica CM3050 S For repacking proceed as per unpacking in structions in reverse order Available models e Basic instrument with sectioning motor Without specimen cooling e Basic instrument Without sectioning motor with specimen cooling e Basic instrument with sectioning motor with specimen cooling Instruction Manual V1 3 03 2008 3 Installation 3 3 Standard delivery md md M M Basic instrument Handwheel with marking antibacterial assesseer 0471 42558 Heat extractor stationary ccccccccsccscsssscssceserssecsesessrssesessesrsensessevensaees 0369 11197 Low temperature stabilizer for heatextractor ow eee eee 0452 27919 90 prism for direct specimen freezing on specimen head with clamping screw to facilitate specimen trimming sresvavrrrerrnnn 0443 25949 ETEN 0470 43550 4 Specimen discs MIM iuasoceonsstencantseatonst dasuseisesseaina qaeadalittonnavedcetaitaienian 0416 19275 4 Specimen discs NN 0370 08587 TT 0443 25723 SOLE EL 0443 33549 Se UO Wi SLO ay EE NE 0400 26817 Foot switch with protective guard uu ccccessscssssrssesssrssesnceteesensenes 0502 29977 FT INA osna E 0443 25732 Freezing shelf COVER siceniosdocavnsnciuenotinnssiennvinnnesinwinnnsaicaundesoosarntilsiusenommnnennanin 0443 30783 NN 0398 13088 DEE e E E ET EE A EE E EEE E EA 0436 43463
21. via the ar row keys When pressing the parameters are dis played in the sequence listed on this page when pressing a they are displayed in re verse order The display can be scrolled contin uously i e after the last parameter the display will automatically start again with the first 1 Refrigeration state CiT 310 C JO T 3 5 C mjajHjojM E I 1 eg Meaning of the refrigeration symbols asterisks gt see chapter 5 3 5 Status indication 2 Section thickness Ci T 3 o fc jolt 35 C The sectioning thickness is set with the or key see chapter 5 4 7 Setting the section thickness 3 Section counter CIT 3 o c Jo T 3 5 C TITT TT TT f amp nfo o o Once displayed via arrow keys the section counter can be setto 0 at any time e To set to 0 press y lf the section counter is not set to 0 by the user at some point sections are counted up to 999 at which point the counter automatically switches back to 0 Leica CM 30508 Cryostat 4 Total section thickness counter C T 8 o C 1o T 3 5 C IT TT TT amp fufofo ofo jo Can also be set to 0 at any time via ov key If the total section thickness indication Is not set to 0 manually the instrument counts up to maxi mum 9999 5 um at which point the counter will au tomatically switch back to 0 This indication shows the total thickness of all sec
22. 419 33981 Anti roll plate QlasSLuLaqnsemeamsnsmeaaanemaniaennnnniis 14 0419 33816 Knife holder CE Iow prof ccccccesssscsessssrssessscsssesescessessnseesesesenees 14 0419 33990 Knife holder CE high prof i mmvererevvrververvrverrerveverververreververserns 14 0419 33991 GE holder CE EEE 14 0419 33992 Pressure plate rear HP 22 Lusnnaesansgamnsandadl eniedjun 14 0502 29553 Pressure plate rear LP 22 mmmmmmemememmenmmmmmnmmnmneenen 14 0502 29551 Anti roll plate assy 70 MM rsrrnvrrrrrenvrrervervrververvenrnververreservervenen 14 0419 33980 Anti roll plate assy 70 MM rsrrnrrrrrrenvrververvrververvevrrververreneevervenen 14 0419 37258 Anti roll plate assy 70 MM rsrrnvrrrrrenverervervrververveververversenesvervenen 14 0419 37260 Anti roll plate glass ssererrerrnvervrnvnververvevrnvervenrenerverveneeverveneenen 14 0419 33813 LEN 14 0419 35693 Knife holder ET vvs 14 0419 32073 Specimen disc 20MM Leese 14 0370 08636 Specimen disc 25MM sieciscrsasacsrsscnarscsstcunnandoensenenssnstinisanbonsietadies 14 0416 19275 Specimen disc JO MM sunne nannestad 14 0370 08587 SPECIMEN disc 10 MM isssvstacssicssvssvsinsvstivanasnecnsdvisesrscvenevssnseusannenat 14 0370 08637 Specimen disc 55 MUM svesincissonidswasianssnssnsisrmeiinontienvesaucneantninasnnlassns 14 0419 26491 Specimen stage snscccssesisadetasisnsssaaetcastutatiansteanzeresietnatanisenninnmeudt 14 0419 26750 He Se EE 14 0416 38207 ge CLO I EEE 14 0443 26836 M
23. 6 Dispensing slides 8 pCS esessssssssssrsrsrsrsnurernrsrurnrrrennrrrererirrrisirirreens 14 0201 39127 Case w embedding SYSTEMS cccccccsecssessecscsessrsseseerssesesersenees 14 0201 40670 Easy Dip staining cnt Wt scassciveiveivecnidssvecnsavcincivnisiaenvsiysaneseranneedacars 14 0712 40150 Easy Dip staining cnt pK rerrorrorrverrorvrrerverrererverresvsververesverververennns 14 0712 40151 Easy Dip staining Cnt grn ER 14 0712 40152 Easy Dip staining cnt VW licsetceseaceptacicansithonstaniaiaivnndnatensicdatamasntnncincaies 14 0712 40153 Easy Dip staining cnt bl m ernernemvrnemivrenivmeneieninn 14 0712 40154 Easy Dip staini rack grey cccccescseseesssessscessesnsessessnseeseresensas 14 0712 40161 Sale y OV OS SIZED EE ne arei EEE 14 0340 40859 Safety gloves size M essessssssserenesersrineririirsininrnrsrsrsrsrsrnrnrnrerrrnnns 14 0340 29011 OCT Lompound 125 EE EE 14 0201 08926 Cryostat oil ENN 14 0336 06100 Leica Cryofect 4x350 ml aac sesteetteteaian anecdauatrarsennae ned toceeaatntuesaraneete 14 0387 42801 63 10 EG Declaration of Conformity Seica JU IJUULIdL at MICROSYSTEMS 8 N L a H a m a ee ee EE 1 fh WY Lf E j ATA 14 gt WeVwraiiwt7 4h DE k A ion OI Goniormity y We herewith declare in exclusive responsibility that the Leica CM3050 S Cryostat was developed designed and manufactured to conform with the e Directive 2006 95 EC of the European Parlia
24. A Fumengs 2059759 number 3 2469 Senee 2807 SF 3 7 lao Microarea usiach GrH mumki Deris Minds n Ty 1 Important information General This instruction manual includes important in structions and information related to the operat ing safety and maintenance of the instrument The instruction manual is an important part of the product It must be read carefully before using the instrument for the first time and must always be kept with the instrument If additional requirements which exceed the scope of this manual are imposed by regula tions and or laws on accident prevention and environmental protection in the country of Operation appropriate instructions for compliance with such requirements must be added to this manual Read this instruction manual carefully before attempting to use or operate the instrument A Please pay particular attention to chapter 2 safety features safety instructions Please read this information even if you are already familiar with the operation and use of other Leica products 1 2 Designated use The Leica CM3050S is a powerful cryostat for rou tine as well as research applications in biology medicine and industry The instrument has been designed for rapid freezing and sectioning of tissue samples The instrument has not been designed for un attended storage of tissue material The instrument may only be operated within the scope of its designated u
25. ED senere ease vost EE 14 0398 18542 AE 14 0443 25949 GS EGO EEE 14 0800 37739 Anti static kit high prof meerernemvrernivemimenemenee 14 0800 37740 Height adj module CM3050 S erresrenveververvevervesvesveverververvesssververns 14 0443 26148 Height adj module CM3050 S rrnrrvrrvrrvrververvrvrrvesesrevesvesvesesvervenen 14 0443 26147 Cryoembed system cpl set nmsmmarvrervvrvvrrerervrverrervrververvrnns 14 0201 39115 Embed well bar set 18mm rnrnnrsrrrrenvnververvesvrvervesveverververrevesversenns 14 0201 39116 Embed well bar Set 24mm rrrrnrrrrrrerveververvesvrververvsverververrevesversenns 14 0201 39117 Embed well bar Set 30mm rsrrnrrrrrvenvrververvesvrvervesveververversevesverserns 14 0201 39118 Freezing griddle elevated freezing block set uo cece 14 0201 39119 Instruction Manual V1 3 03 2008 9 Appendix Leica CM3050 S Cryostat Embedding well bar 4X 18MM rrnrsrrvrvvrrervrnerverveserververreververvesernes 14 0201 39120 Embedding well bar 4x24MM rrrvrsrrrvnvvrrervrnervervesesververseververvesennes 14 0201 39121 Embedding well bar 3x30MM rrrrnrrrvrvvrrervrnerrerveserververseververvesernns 14 0201 39122 SPECIMEN stage square 28MmmM nrnsrnvrverrervrververrerervervesvevrrvervensene 140201 39123 SPECIMEN stage square 36mmM rnsnnnnrververveververveservervesvevrrvervesene 140201 39124 Ovewr disc heat extractor muemumnemnememenimenemieninn 14 0201 39125 BNI e e E E E E A 14 0201 3912
26. Kuvaas aeenknaadene dar 57 Warranty and service sassssssususnununnnuununnunununnununnnnunnnnunununnununnunnnnnnunnnnnnnnnnnunnnnunnnnnn ennan 58 Appendix ve nannan 59 9 1 Electrohydraulic height adjustment ssesesessssesererensrerereneernrriniersrurununununnnnnnnunnnnnnnrnrurenunnrnrnrnrnsnnnnas 59 TEI MOS SIO VE E E E A E E 59 SN secrenosranien AEE 59 913 ENN ne 59 SEE EE NE ENN 60 NNN 61 9 1 6 Maintenance and Care ssrsrsvarerrerverrenrsnerverrenrsververserresesvesverrersesssserverrerssvesversessesessenvervenrssenvervenenne 61 Eee See NE NE 61 RE EE Aes AD EEE EE 61 9 2 Ordering information ACCOSSONES Javed 62 EC Declaration of CGonformity armarasvravnnvnnvnnvnnvnnvnnvnnvnnvnnennennennennennnnnennennennnnnennennnnnns 64 Decontamination Certificate master u mnsvnavnavnsvrnvnaennvnnvnnensennennennennennennennnnnennennennennnn 65 Information for the People s Republik of China nesornrnenoenrnrnvnnrnvnennrnrnenvrnrnenenn 67 Instruction Manual V1 3 03 2008 1 Important information The chapters of the Leica CM3050 S cryostat instruction manual Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 Chapter 6 Chapter 7 Chapter 8 Chapter 9 Chapter 10 Chapter 11 Chapter 12 Structure of the manual e Table of contents e Important information on this manual Safety e Read this chapter before operating the instrument Installation e Unpacking and installation e Standard deli
27. NBU ME 0183 28642 1 Leica brush w magnet iemerrsvererverrererververvrververessrvenverververesverrenen 0183 40426 TSN 0222 10050 TE NE 0222 04137 1 Allen key aan 0222 04138 1 Allen key Size 4 0 sccccsiceestodysantennastnsvuinsi slvstinasubeaussadenensussdeansendiverhvountesss 0222 04139 1 Allen key with spherical head size 4 0 0 cesses 0222 32131 1 Allen key EN 0222 04140 1 Allen key with handle size 5 0 assesseer 0194 04760 1 Allen key size 6 0 nsesranrrnevrnvervenesvesvesvenvsvesvessevesvessesvsvesvesvessenesvesvenen 0222 04141 1 Single head wrench SIze 13 rnnarrrrenrererververvnververvevrrvenvervevervesverrenen 0330 33149 1 Single head wrench size 16 rsrrsrarerrrververvnververvenrnvervesvesesvesvenvenen 0330 18595 Bottle of OCT Compound mounting medium TRINN 0201 08926 Bottle of cryostat oil type 407 50 Ml eererrnnrrvrrvenvnrerverrenvrvervesvenrrverveneene 0336 06098 Pair of cut resistant gloves SIZE S mmmumvrvremvrererierereneniinen 0340 40859 Instruction manual Leica CM3050 S G E F S eeens 0708 37109 Leica CM 30508 Cryostat 17 3 Installation In addition to the above e Instruments with specimen cooling 1 90 Prism for low temperature quick freezing e Configured instruments 1 Knife holder base 1 Knife holder with accessories e Instruments with sectioning motor 1 Footswitch with protective guard Further accessories Further accessories which you order
28. Next steps see chapter 5 4 6 Trimming Sectioning e Adjust knife knife holder relative to speci men see Knife holder instruction manual e Place anti roll guide onto knife e Orient specimen if necessary see chapter 5 5 5 Specimen orientation e Select desired section thickness see chap ter 5 4 7 Setting the section thickness e Start sectioning at approx 20 um reduce section thickness gradually until reaching the appropriate thickness for the type of specimen to be sectioned e Discard the first 2 to 3 sections cut at the de sired section thickness When sectioning manually rotate the hand wheel evenly and at uniform speed For all details on motorized sectioning please refer to chapters 5 4 1 to 5 4 4 Selecting a sectioning mode Start and stop sectioning Setting the sectioning window Selecting sectioning speed Emergency stop For advice on appropriate Sectioning tempera tures for different types of tissue please refer to chapter 6 3 Reference chart for temperature settings 43 5 Operation 56 Finishing work 5 6 1 Daily routine steps Remove all specimens from the cryochamber and store safely See also corresponding safety instruc tions in chapter 2 3 7 Defrosting han dling frozen tissue e Lock handwheel e Remove knife from knife holder disinfect dry see chapter 7 Disinfection cleaning and maintenance and st
29. Set specimen temperature Is displayed SJE T TIE MIP Jo T IC Press key briefly iy The line in the specimen temperature field is replaced by an asterisk 3 SJEIT TEMP OTI 1 C PE tT ETE et et tty TE From this point on the refrigeration sys A tem is activated and the desired set temperature can be selected with one of the arrow keys Press arrow key and release upon reaching the desired value epT J 1 40 Jo T f3 5 C mju Hjo M E Tt tt gt Note on the arrow key function Each time one of the arrow keys is pressed briefly the value displayed in the menu will be changed one step up or down If an arrow key is pressed and held the value will change continually auto repeat function When holding the key scrolling speed will in crease after a while s soon as a value Is displayed it is at the same time automatically stored 29 5 Operation 2 Cryochamber temperature a Press key until menu item Set chamber temperature is displayed gt Continue as described for specimen temperature 3 Preset counter instruments with sectioning motor only Press key until menu Item Preset counter Is displayed N tans JP RJE SJEIT CJO UJN TJEIR Preset counter activated JP RJE SJEIT CJO UJN TJEIR Preset counter deactivated Preset counter function The preset counter allows the user to carry out a p
30. TEMS Issued by Leica Biosystems Nussloch GmbH Heidelberger Str 17 19 D 69226 Nussloch Germany Phone 49 6224 143 0 Fax 49 6224 143 200 Internet http www histo solutions com Leica CM 30508 Cryostat 3 Table of Contents 1 Important information rs srnvenenvnvrnvnvnvrnvnvnernvnvnvrnvnvnernvnenenennenenennenenennennnernenenerernenenernenn 1 1 Symbols used in this manual and their meaning sreevrsvevvrrervervenrenvnververrenrrnernervervesveververserrenesnenne 1 W NCSU EE EE EE 8 1 3 Qualification of personnel EE nnna 8 Dis STN ren 9 2 1 General information on instrument design and safe handling soervrrrrnvenvrververvenrrnerverververennen 9 2 2 Integrated safety devices n srerrenrnnrrverrerrervenvrververrenrenenserrervenenververreressesverversenrssenserrerresesvervesreseenenn 9 2 2 1 Locking the handwheel serrrerrenverorrerrervenveververvenrsnerververrevesverversesssnesvervesvenssserservesssvesversesssvesvenne 10 2 2 2 Centering the handwheel grip rrorrvrverrervrnvererverrenvrnvnvenvenrenrrnerverrerrenesverversersesesvensesressssesverrenenne 10 2 2 3 Emergency stop function instruments with sectioning motor only ireseserrererverererrerevenrene 11 EE GE ET EEE EE EN 11 2 3 Safety instructions handling and operation of the instrument ssernevaververrervenverervervenvenenne 12 TTER 12 292 SN endene E 12 50 EMS 12 2 3 4 Handling microtome knives blades
31. bile heat extractor is available as an optional accessory see Ap pendix Ordering information accessories Store heat extractor in chamber to precool e Place onto specimen in quick freeze shelf e Remove as soon as specimen Is frozen 41 5 Operation Thermoblock The thermoblock makes it easy to remove fro zen specimens from the specimen discs 42 Do not store the thermoblock inside the cryochamber store at room tem perature and only place into the cham ber for removing specimens from discs Place cap 9 on one of the two ends so that the correct size opening 7 for the disc to be inserted is exposed Insert pin 6 of specimen disc 5 into the opening 7 After about 20 seconds remove the frozen specimen from the specimen disc use tweezers If cap 9 fits too loosely readjust with screw 10 Do not tighten screw too much Once the specimen has been detached from the disc remove the thermoblock from the cryochamber 5 5 4 Inserting specimen discs into the specimen head Lock handwheel in upper position If a knife has already been inserted into the knife holder cover knife edge with knife guard Loosen clamping screw 3 Insert specimen disc in opening 2 rear surface of specimen disc must fit tightly against surface of specimen head Tighten clamping screw 3 5 5 5 Specimen orientation Open clamping lever 4 gt 4a Orient
32. by pressing 4 For further information on the preset counter please refer to chapter 5 3 6 Item 4 Preset counter Instruction Manual V1 3 03 2008 5 Operation 4 Quick freezing at maximum specimen temperature instruments with specimen cooling only Press key until menu item Fast object fe freezing is displayed HJAfS T Opes JFJRJEJEIZ TIN FT TT loffe FT TT TT FJA SJT fopp y FIRE El Z 1 TI TTTT lon TT Press lock unlock key to activate or de activate ON OFF 5 Real time Press key until menu item Time is dis played ry a tans TIME 10 213 e Use arrow keys or to set real time Setting minutes as well as hours in 1 minute steps Holding arrow button in creases scrolling speed 6 Starting time of automatic defrost cycle Press key until menu item Defrost time is N displayed DJEIFIR Tf MJE 14 13 Time on display start time of defrost cycle e Use arrow keys 4 or to select the de sired defrost start time with 90 prism see also chapter 5 5 2 Leica CM3050 S Cryostat The automatic defrost cycle takes place once every 24 hours at the selected defrost time 7 Duration of defrost cycle Press key until menu item Defrost period is displayed DJEJF RJO SJT PJEIR HOD ease CIHJAIM BJEIR f 0 8 M I N e Use defrost keys 4 or y to adjust de frost t
33. ded period of time gt In case of power failure or instrument failure or during the automatic defrost cy cle tissue material can be destroyed During the defrost cycle the cryochamber Is partially warmed Therefore e Remove sensitive specimens from the chamber prior to defrosting e If automatic defrosting is programmed to take place during the night remember to remove all specimens from the cryo chamber prior to leaving work 2 3 8 Frozen parts of the instrument and frozen accessories Prolonged contact of bare skin to frozen surfaces of the instrument or to frozen accessories Specimen discs knife holder shelves etc can cause frostbite e Wear protective gloves 13 2 Safety 2 3 9 Infectious radioactive material 14 Danger Use caution when working with potentially infectious specimens gt Risk of infection When working with potentially infectious radioactive specimens e Wear protective clothes gloves protec tive boots mask lab coat in compliance with radiation safety regulations and or in house regulations on handling infec tious radioactive material When working with radioactive specimens e Comply with applicable radiation safety regulations e Dispose of radioactive specimen waste ac cording to applicable regulations 2 3 10 Disinfection and cleaning Prior to disinfection switch the instrument off and unplug It from mains For removal of the micr
34. downward at the left side to remove it from fastening clip 5 Pull the lamp towards the left to remove It from its socket 6 Installing the replacement lamp Leica CM 30508 Cryostat Hold the lamp in correct position see draw ing below and push towards the right until it locks into the socket Push the lamp slightly upwards until it locks into the clip Put the sliding window back in place Reconnect the instrument to mains and switch it back on 57 8 Warranty and service Warranty Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control proce dure based on the Leica in house testing standards and that the product is faultless and complies with all technical specifications and or agreed char acteristics warranted The scope of the warranty is based on the content of the concluded agree ment The warranty terms of your Leica sales organization or the organiza tion from which you have purchased the contractual product shall apply exclusively Technical service information lf you require technical service or replacement parts please contact your Leica sales representative or dealer who sold the product Please provide the following information Model name and serial number of the instrument Location of the instrument and name of the person to contact Reason for the service call Date of del
35. e changed while trim ming Is In motion by pressing or Leica CM 30508 Cryostat 5 4 7 Setting the section thickness e Activate sectioning mode mam off GIT J 90 10T 1 11 21 e Press or to select section thickness Pressing one of these keys once makes the display in control panel 1 switch to section thickness indication thickness value not yet being altered Pressing or further starts to change the actual section thick ness setting Selectable section thickness settings 0 2um in0 5 um steps 2 10um in 1 um steps 10 20um in 2 um steps 20 60um in 5 um steps 60 100um in10 um steps 100 300um in50 um steps 39 5 Operation 5 5 Daily operation of the instrument Select chamber temperature and in instru ments with specimen cooling specimen temperature according to the type of speci men to be sectioned see also chapter 6 3 Reference chart for temperature settings Do not continue with step 5 5 1 before the actual chamber specimen temperature corresponds to the set value This may take several hours e g when operating the in strument for the first time or when the instru ment had been switched off Prior to operating the instrument 40 please read and observe the following safety instructions e Integrated safety devices Chapter 2 2 e Handling microtome knives blades Chapter 2 3 4 e Knife guard Handwheel lock
36. ed will be included in the box contain ing the standard delivery items separate instruction manual In case of non configured instruments the knife holder is not a part of standard delivery but must be ordered separately i Knife holders are delivered with anti roll guide knife guard and a e Check all delivered parts againstthe packing list and against your order to verify whether the delivery is complete lf there is any difference contact your local Leica office immediately 18 Instruction Manual V1 3 03 2008 3 Installation 3 4 3 4 Installing inserting accessories 1 Installing the handwheel Insert pin 1 of the handwheel shaft into hole 2 Place spring washer 3 onto screw 4 as shown Tighten screw 4 with an Allen key Cover with selfadhesive disc 5 For purposes of transport e g narrow doors the handwheel can be removed To remove the handwheel proceed as de scribed above but In reverse order Leica CM 30508 Cryostat 3 4 2 Inserting the accessories i Place the rubber mat on top of the housing Insert the storage shelves into the cryo chamber Install the stationary heat extractor into the quick freeze shelf see also drawing in chapter 5 5 1 Freezing specimens onto specimen discs Insert the low temperature stabilizer into the quick freeze shelf it must be located in the pivoting range of the heat extractor see also drawing i
37. ents with specimen cooling When defrosting starts to avoid icing the spec imen head is automatically adjusted to a tem perature between 10 C and 5 C i e the set temperature value is deactivated temporarily same as with automatic defrost cycle see chapter 5 7 1 Automatic chamber defrost cy cle 10 seconds after the manual chamber defrost cy cle has been completed the specimen head re frigeration is switched back on cooling down to the set value 46 5 7 3 Defrosting the specimen head instruments with specimen cooling only To activate specimen head defrosting e Press until menu item Set specimen temperature Is displayed SJEIT TjelM P Jo t 3 5 C TITT TTTTTTTT TT fele e Press AA A brief sound signals that the manual chamber defrost cycle has started SJEIT TEMP O T 3 5 c PEt PTET Ee TE leE Specimen head temperature increases to be tween 20 C and 30 C for a duration of 10 minutes after which it returns to the set value To abort specimen head defrosting e Press until menu item Set specimen temperature is displayed and press once more Instruction Manual V1 3 03 2008 6 Troubleshooting applications tips 6 1 Displayed error messages The autodiagnosis function of the CM3050 S displays error messages In case of certain mal functions 1 Power failure This error message Is displayed after the instru ment had been disconnected fr
38. g handwheel 882 mm Depth 766 mm Height arm rest level 840 mm Overall height 1040 mm Weight incl microtome w o specimen cooling ca 180 kg according to IEC 1010 UL 3101 according to CECOMAF Liquid temperature 45 C Evaporation temperature 25 C Instruction Manual V1 3 03 2008 5 Operation 5 1 Operating the instrument for the first time Prior to setting up the instrument make sure to read the instruction manual carefully and in full When setting up the instrument please follow the individual chapters in the following order Leica CM3050 S Cryostat First study chapters 1 2 and 3 install the instrument as described in chapter 3 The overview in chapter 4 1 is helpful during the unpacking and instal lation procedure in order to identify individual parts and place them in the correct location Go on to chapter 5 Read chapters 5 1 5 2 5 3 and 5 4 to learn all about the controls Next start to work with the instrument chapter 5 5 Follow the instruc tions in chapter 5 5 Chapter 5 5 2 applies to instruments with specimen cooling only The temperature chart in chapter 6 3 is useful when It comes to select ing appropriate sectioning temperatures for different types of speci mens When disinfecting cleaning the instrument and or removing rein stalling the microtome please follow the instructions in chapter 7 In case of problems and malfunctions please refer to chapters 6
39. ime Only if necessary see below Important information for setting the defrost parameters Starting time of defrost cycle We recommend to program the automatic defrost cycle for a time out side regular working hours as a period of 4 hours should pass after the defrost cycle before work with the instrument is resumed see also chapter 5 7 1 Automatic chamber defrost cycle i Defrost cycle duration The duration of the chamber defrost cycle is set to 8 minutes in factory This setting should only be changed if the evaporator is not completely de iced during the 8 minute cycle In such cases increase the defrost cycle duration gradually until the the evaporator is ice free after defrosting The selected defrost cycle duration is valid for both automatic and manual chamber defrost cycle 31 5 Operation 5 3 5 Status display Each individual parameter Is displayed in a certain area of the display screen Upper line In the menu see chapter 5 3 4 indication of selected menu item Standard indication Chamber temperature instruments with specimen cooling chamber and specimen temperature Standard indication upper line Instruments with chamber and specimen cooling CIT 3fo C o T 3 5 C mf Hjolm e I IT ee Upper line Lower line Digits 1 and 2 indicate sectioning stroke position DE E Sectioning stroke outside sectioning window
40. ing the microtome e Removing reinstalling the microtome Chapter 2 3 11 e Display message Dry Microtome Chapter 2 3 12 Lock handwheel in lower position Place microtome in chamber Put one drop of cryostat oil per surface on the contact surfaces of plastic coupling 11 and handwheel coupling 12 Place plastic coupling 11 on handwheel coupling 12 Push microtome slightly towards the right until axle 9 fits tightly into coupling 11 Unlock handwheel Adjust the parts into each other by rotating the handwheel slightly backward and forth Lock handwheel again in lower position Tighten screws 8 Reconnect plugs of coarse feed motor 6 and temperature sensor 7 Mount specimen head in reverse order as described on previous page Insert accessories storage shelf section waste tray etc Insert sliding window Connect instrument to mains and switch back on Instruction Manual V1 3 03 2008 7 Disinfection cleaning and maintenance 7 6 Exchanging the fluorescent light Removal lamp Prior to exchanging the lamp please read and observe the following safety instructions e Maintenance j Chapter 2 3 13 Removing the sliding window e Hold sliding window 1 at grip bar 2 lift slightly and pull out towards yourself The lamp 3 is located behind the glare shield 4 Carefully hold lamp with both of your hands Slightly pull the lamp
41. ion thickness selection Ea pa RUN RUN STOP ENABLE STOP C Functional area C e Start Stop sectioning 34 Instruction Manual V1 3 03 2008 5 Operation Functional areas A and C Instruments with sectioning motor only 5 4 1 Selecting a sectioning mode start and stop sectioning Three different sectioning modes can be select ed for motorized sectioning Sectioning mode 1 Intermittent stroke The specimen head travels up and down as long as the RUN STOP and RUN ENABLE keys are pressed simulta neously or as long as the footswitch is depressed Sectioning mode 2 Continuous stroke I The specimen head travels up and down until it is stopped by pressing RUN STOP or RUN ENABLE or by depressing the footswitch Sectioning mode 3 Single stroke LT The specimen head carries out one sec tioning stroke and then stops in the low er limit position Activating a sectioning mode Press one of the three sectioning mode keys de scribed above While a sectioning mode Is ac tive the LED in the corresponding key will be il luminated Leica CM 30508 Cryostat Start stop motorized sectioning Sectioning mode 1 Intermittent stroke e Simultaneously press and hold RUN STOP and RUN ENABLE or depress and hold footswitch e To stop the sectioning stroke release the keys or the footswitch The specimen head stops immediately Sectioning mode 2 Continuous stroke e Simultaneou
42. ise Always set the sectioning window while in the sectioning stroke speci men moves downward Setting the window in retraction stroke specimen travels upward is not possible Press sectioning window key l Position upper edge of specimen just below the knife edge continue to rotate the hand wheel clockwise Press sectioning window key once more The value has been stored when the LED in the sectioning window key lights up briefly If the LED flashes the value has not yet been stored because the user has tried to set a win dow margin outside the possible window range the area in which the sectioning window can be set is slightly smaller than the total section stroke e n that case repeat the step in question i The sectioning window cannot be de leted If need be it can be set to maxi mum size i e almost the entire sec tioning stroke will be carried out at one and the same speed Every time after the instrument is dis connected from mains the sectioning window is set to maximum size upon switching back on This also applies when the instrument is switched on for the first time Instruction Manual V1 3 03 2008 5 Operation Functional areas A and C Instruments with sectioning motor only 5 4 3 Selecting sectioning speed e Sectioning speed is selected via the sliding potentiometer Speed can be changed while sectioning Is in motion Caution whe
43. ivery Decommissioning and disposal The instrumentor parts of the instrument must be disposed of in compliance with the local laws 58 Instruction Manual V1 3 03 2008 9 Appendix 91 Electrohydraulic height adjustment An electrohydraulic lifting device for individual working height adjustment is available as an optional accessory for the CM3050 S cryostat series 9 1 1 Designated use The electrohydraulic height adjustment device has been designed exclusively for adjustment of the instrument working level to the individual height of the user It has been designed for use with the Leica cry ostat series CM3050 S only and may under no circumstances be used to lift other instruments or persons Damage resulting from improper use of the product and or faulty operation invalidates the warranty Leica will not assume liability for any such dam age 9 1 2 Standard delivery Electrohydraulic height adjustment device for cryostat series CM3050 S complete outfit con sisting of a U shaped frame on four height ad justable feet and a two key control unit The height adjustment device is available in 2 voltages 1 230 240 V 50 Hz order no 0443 26147 2 230 240 V 50 Hz order no 0443 26147 Leica CM 30508 Cryostat A 9 1 3 Safety instructions Prior to operating the height adjustment device make sure to read the present instruction manu al Pay special attention to the following safe
44. kK Cartilage sk fe Cervical ske Fatty sk Heart and vascular Intestinal sk Kidney sk Laryngeal sk Lip sk sk Liver sk Lung sk Lymphoid Muscular Nose sk Pancreatic Prostate Qvarian KV Re oe Rectal Skin with fat sk Skin without fat Spleenal or bloody tissue sk Testicular sk Thyroid Tongue sk Uterus curettage sk ed RE BF Please note The recommended temperature settings are based on long term experience However they can only be guidelines for general orientation as each individual specimen may require particular adjustments 52 Instruction Manual V1 3 03 2008 7 Disinfection cleaning and maintenance 7 1 Safety instructions on disinfection 1 2 Spray disinfection with Leica and cleaning Cryofect 7 l For practical spray disinfection we recommend When disinfecting the instrument Leica Cryofect which is fully effective at tem make sure to observe the following 0 EE Sure to Fast de lie peratures in the negative range down to 20 C safety instructions e Disinfection and Cleaning e Set chamber temperature 20 C or warmer Chapter 2 3 10 e Remove specimens from the chamber and l l l store safely e Handling microtome knives blades Chapter 2 3 4 e Carefully remove knife blade from knife holder e Defrosting handling frozen tissue e Remove section waste tray from cryocham
45. ld up inside the micrometer mechanism Microtome defective Remove microtome from cham ber and dry possibly with hair dryer hairdryer only to be used outside the cryochamber or at ambient temperature Call Technical Service Specimen disc cannot be removed Specimen disc stuck to quick freeze shelf or specimen head due to moisture on underside of specimen disc Apply strong alcohol to contact point or heat the specimen head Cryostat inoperational Mains plug not properly con nected Automatic cut out has been triggered Check mains plug connection Reconnect automatic cut out switch instrument back on Sectioning motor inoperational Footswitch not connected Automatic cut out of sectioning motor has been trig gered Motor defective Connect footswitch Reconnect automatic cut out push inwards until it locks in place Call Technical Service No or insufficient refrigerating performance Leica CM 3050 S Cryostat Stopper not placed properly into drain in bottom of cryo chamber Compressor defective Leak in cooling system Close drain with stopper Call Technical Service Call Technical Service 51 6 Troubleshooting applications tips 6 3 Reference chart for temperature settings in minus C Tissue 10 15 15 25 25 50 Adrenal Bone marrow kK Brain Bladder Breast fatty sk Breast little fat
46. ll the institution which owns the in strument and in addition the designated per sonnel who operate service or repair the instrument 2 Safety 2 2 1 Locking the handwheel A Always cover the cutting edge with the knife guard and lock the hand wheel e Prior to doing any work on knife and or specimen e Prior to exchanging specimens e During work breaks The handwheel can be locked in 2 positions e with the grip in the uppermost position left e with the grip in the lowest position right Locking e Rotate handwheel until grip 1 is in upper or lower position e To lock press pin 2 to the right into position 2b gt The upper locking position for pin 2 is marked by a black dot 4 10 Instruments with sectioning motor The sectioning motor is now blocked All instruments The message LOCKED in the display of control panel 1 indicates that the handwheel has been locked cT 310 c Jo T 3 5 C J ltfolekep TITT Unlocking e To unlock push locking pin 2 to the left into position 2a e Display indication LOCKED disappears Instruments with sectioning motor The sectioning motor can now be activated again 2 2 2 Centering the handwheel grip A e To center grip 1 pull outwards and pivot into center of handwheel During motorized sectioning for safety reasons always center the handwheel grip
47. mains make sure the electrical power Supply in your laboratory corresponds to the values indicated on the instrument nameplate located at the rear of the in strument Instruction Manual V1 3 03 2008 2 Safety 2 3 4 Handling microtome knives blades Danger gt Microtome knives and disposable blades have extremely sharp cutting edges and can cause serious Injuries Therefore e Handle knives blades with utmost care e Never leave any knives blades in unpro tected places e Never place a knife no matter where with the cutting edge facing upwards e Never try to catch a falling knife e Always insert the specimen before inserting the knife 2 3 5 Knife guard handwheel lock 2 3 6 Always cover the cutting edge with the knife guard and lock the handwheel e Prior to doing any work on knife and or specimen e Prior to exchanging specimens e During work breaks Motorized sectioning Do not interrupt sectioning trimming by set ting the sliding potentiometer to zero speed gt This does not really switch the sectioning function off it only operates at 0 speed If the sliding potentiometer is accidental ly moved the instrument will resume sec tioning immediately risk of injury During motorized sectioning always center the handwheel grip Leica CM3050 S Cryostat 2 3 7 Defrosting Handling frozen tissue Never leave specimens unattended in the cryochamber over an exten
48. mber illumination changing instrument parameters To select the menu items for setting In the status indication pressing the ar row keys displays the individual current parameters VI In the menu pressing the arrow keys changes set values As soon as a value Is displayed it is au tomatically stored Cchambe the manual defrost cycle cry AA chamber This key ts functional only when one of the menu items SET TEMP CT C or SET TEMP OT C last one only applies to instruments with specimen cooling has been selected first Lock unlock key Locks the display to protect against accidental changes of set parameters Activates deactivates a number of functions 3 Leica CM3050S Cryostat 5 3 2 Display functions in control panel 1 The display in control panel 1 combines several different functions 1 When switching the instrument on A series of standard indications is displayed during which two basic parameters can be se lected with the arrow keys Language of display indications and speed level of sectioning motor See chapter 5 3 3 Display indications when switching on 2 Menu function activated via menu key Via the menu key a number of parameters can be selected then be activated deactivated via the lock unlock key and or changed via the ar row keys See chapter 5 3 4 Menu functions Setting param eters of refrigeration time and preset coun
49. ment and of the Council Low Voltage e Directive 2004 108 EC of the European Parliament and of the Council electromagnetic compatibility e Direktive 98 79 EC of the European Parliament and of the Council in vitro diagnostic medical devices The following harmonized standards were applied e EN 61010 1 2001 Safety requirements for electrical equipment for measurement control and laboratory use Part 1 General requirements e EN 61326 1 2006 Electrical equipment for measurement control and laboratory use EMC requirements Part 1 General requirements e EN 61010 2 101 2002 Safety requirement for electrical equipment for measurement control and laboratory use Part 2 101 Particular requirements for in vitro diagnostic IVD e EN 14971 2007 Medical devices Application of risk management to medical devices e EN591 2001 Instruction for use for in vitro diagnostic instruments for professional use e EN13850 2006 Safety of machinery Emergency stop Principles for design e EN1037 1995 Safety of machinery Prevention of unexpected start up In addition the following in house standards were applied e DIN EN ISO 9001 2000 Leica Biosystems Nussloch GmbH Postfach 1120 i s D 69222 Nussloch March 10 2008 President Biosystem Devision 64 Instruction Manual V1 3 03 2008 11 Decontamination Certificate master Dear Customer any productthatis to be returned to Leica Microsystems or serviced on site
50. n chapter 5 5 1 Freezing spec imens onto specimen discs Insert section waste tray and brush shelf Install knife holder base onto microtome base plate and clamp Install knife holder and clamp see knife holder instruction manual for details Place knife case with knife into chamber to precool Place all tools needed for section prepara tion into the chamber Close the sliding window For a complete overview of all individual parts gt see Chapter 4 1 Overview 19 3 Installation 3 4 3 The footswitch Connecting the footswitch e Insert footswitch into port 1 and secure Function 1 The footswitch performs the same task as the RUN STOP and RUN ENABLE keys activating 3 5 Prior to switching on the instrument deactivating motorized sectioning trimming In addition the footswitch can be used to acti After transport observe a waiting pe vate the emergency stop function riod of at least 4 hours before turning the instrument on See also safety in structions in chapter 2 3 1 Transport Models with footswitch All instruments with sectioning motor 1 Have you observed all safety instruc tions in chapters 2 3 2 Site require Important note ments and 2 3 3 Electrical connec In all instrument models that are tions delivered with footswitch the If not footswitch must be installed gt Please read chapters 2 3 2 and Mie
51. n working in continuous mode If the slider is set to 0 the sectioning motion stops however the actual sectioning mode is still active just operating at 0 speed If the slider is accidentally moved sectioning will be resumed Risk of injury Therefore Do not interrupt sectioning trimming by setting the sliding potentiometer to zero speed See also safety instruction in Chapter 2 3 6 Motorized sectioning A Vmax key As long as the button is pressed the sectioning motion is carried out at maxi mum speed 210 mm s While Vmax is active the sectioning window is inactive When the Vmax key is released the sectioning window becomes active again Leica CM3050S Cryostat 5 4 4 Emergency stop e Activate by e either pressing the emergency stop button forcefully must lock in position e or depressing the footswitch force fully Either way sectioning Is interrupted at once In both cases the red sr LED in functional area A lights up indicating that the emergency stop function has been activated Simultaneously all 3 sectioning modes are deactivated all 3 LEDs off e To deactivate the emergency stop function rotate the button in the direction of the ar row until it unlocks and slips upward into its Original position As soon as the emergency stop button has been unlocked the red sro LED is extinguished e fthe emergency stop function has been ac ti
52. ns Automatic chamber defrost cycle Manual chamber defrost cycle Specimen head defrosting manual instru ments with specimen cooling only A 5 7 1 Automatic chamber defrost cycle Read the safety instructions in chapter 2 3 7 Defrosting frozen specimens Make sure to remove sensitive speci mens from the cryochamber before the defrost cycle starts The automatic defrost cycle is carried out once every 24 hours atthe preselected time For details see chapter 5 3 4 Menu functions items 6 and 7 During the defrost cycle the corresponding as terisk is displayed inversely CIT 3 o e c 0 T 13 5 C mjajHjojM Ej 11 Pe Once the defrost cycle is finished the instrument automatically switches back to refrigeration Instruments with specimen cooling When defrosting starts to avoid icing the speci men head Is automatically adjusted to a temper ature between 10 C and 5 C i e the set tem perature value is deactivated temporarily Leica CM 30508 Cryostat The specimen head refrigeration is automatical ly switched back on when e atleast 4 hours have passed since the de frost cycle was completed e and the actual chamber temperature devi ates less than 5 K from set value After the specimen head refrigeration has been switched back on the specimen head tempera ture rises briefly to 10 C and then decreases until it reaches the set value
53. nstrument only transport in an upright position e After transport wait at least 4 hours be fore turning the instrument on gt The compressor oil may have been displaced during transport and must settle to its original position be fore switchingthe instrument on Otherwise the instrument may be severely damaged 2 3 2 Site requirements 12 Do not operate the instrument in rooms with explosion hazard To ensure proper instrument function main tain a minimum distance of 10 cm between walls and or furniture and all sides of the in strument 2 3 3 Electrical connections Do not use extension cords for connecting the instrument to mains gt Fire hazard gt Instrument malfunctions caused by voltage drop During the start up phase of the compressor the nominal voltage must not drop below the values specified in chapter 4 2 Technical data gt The compressor needs a start up current at between 25 and 35 see chapter 4 2 Technical data Ensure uniform current supply according to specifications gt Electrical power supply deviating from specifications damages the instrument e Therefore arrange for the electrical in Stallations on site to be checked by a trained professional and make sure any necessary upgrades are installed e Have the circuit protected by a fuse of its own e Do not connect any other consumers to the same circult e Prior to connecting the instrument to
54. om mains either due to power failure or because the mains switch had been turned off CIT 3J0 C 01T 3 5 c I PJO WJEIR FIA JLJU R E e To acknowledge the message press y for about 5 seconds 2 Coarse feed Coarse feed malfunction has occurred GIT 3 0 C Jo T 3 5 0 ASIASAT This message is also displayed if the coarse feed plug has not been reconnected properly after reinstallation of the microtome 3 Temperature indications If the symbol is displayed instead of chamber or specimen temperature Indication the corre sponding temperature sensor is broken or a short circuit has occurred Leica CM 30508 Cryostat 1 Service GIT 3 0 C JO T 3 5 C IsSjelrjvDplicfe 111 This message is displayed after a cer tain number of operating hours to re mind the user to have a preventive maintenance done by Leica Technical Service This message is displayed only if the instrument is not regularly serviced by Leica Technical Service We recommend a maintenance con tract once the warranty period has ex pired Contact your office for details If one of the error messages listed un der 2 3 and 4 is displayed please con tact Leica Technical Service local Leica sales 6 1 1 Other error indications sroe red in control panel 2 is illuminated e Emergency stop button accidentally activat ed gt Unlock e Footswi
55. ore in the knife case e Use a cold brush to sweep frozen sectioning waste into the section waste tray e Empty section waste tray dispose of sec tloning waste according to the applicable regulations on disposal of special category waste e Ifnecessary carry out a complete disinfection procedure see chapter 7 Disinfection cleaning and maintenance e Remove all specimens from the chamber and store safely e Close sliding window e Switch off cryochamber illumination e Use lock unlock key to lock display in control panel 1 Display background dark locked MG WEIS GAO EE ae ERROR Do not switch off mains switch auto matic cutout see chapter 5 2 Switch ing on fuses 44 5 6 2 Switching off the instrument for a longer period of time If you do not plan to use the instrument for an extended period of time it can be switched off However since this involves a lot of work see below it should be considered carefully whether switching off the instrument is really the better option If the instrument is switched off the microtome must be removed Then the microtome cryo chamber and all accessories must be disinfect ed and dried e For that purpose follow the instructions in chapters 7 1 through 7 5 3 Instruction Manual V1 3 03 2008 5 Operation 5 7 Defrosting The Leica CM3050 S has 3 instruments without specimen cooling 2 different defrosting optio
56. otome from the cryo chamber see chapter 2 3 11 Removing the microtome For disinfection wear protective gear gloves mask lab coat etc For disinfection only use alcohol based dis infectants Do not use solvents xylene acetone etc for cleaning or disinfection Do not spray disinfectants into the evapora tor gt Risk of icing Explosion hazard when working with alcohol Make sure the premises are appropriately ventilated When using disinfectants and detergents comply with all safety instructions supplied by the manufacturer of the product Dispose of waste liquids from disinfection cleaning as well as of sectioning waste ac cording to applicable regulations on disposal of special category waste Disinfected accessories must be thoroughly dry when reinserting them into the chamber gt Risk of icing Make sure the chamber is completely dry before switching the instrument back on gt Explosion hazard through alcohol vapors gt Risk of icing Instruction Manual V1 3 03 2008 2 Safety 2 3 12 Display message Dry microtome 2 3 11 Removing reinstalling the microtome Before removing the microtome Switch instrument off Unplug from mains Place handwheel grip in lowest position and lock gt When removing the microtome the specimen head must always be lok ked in the lowest position Otherwise the upper part of the slot cover might be bent and consequentl
57. r to switching on the instrument Luneuaraakesenneskeukkkednasunandisagsg mingdi 20 Instruction Manual V1 3 03 2008 Table of Contents 4 Instrument Properties rsrnrnvrvrnvnvnnvnvnvnvvnenenernvnvrernenenernenenernvnnnernenenerernenernnnenerernenenenn 21 A 6 EEE ER ee ee 21 Te EEE EN 23 EEE EE EE ne 25 5 1 Operating the instrument for the first time sesrrnvnnrrrerranvnvenverrenrenernerverrenrsnerververvesesverversenvenenne 25 5 2 OWitehing SEE EN 26 093 NE 21 5 3 1 Key functions IN control panel 1 ssesesronvrnvrrerrenrenvnverververvarerverversenerserversesresersersersesssvesversensenenne 21 5 3 2 Display functions in control panel TN vuanesnmnemuanandantennaidekj mmudnminn divdtnepoidi 21 5 3 3 Display indications when switching On Lummememsrmeasmmmsngsmennnan imnrdere 28 5 3 4 Menu functions setting parameters of refrigeration time and preset counter ess 29 DA E E E E E E E E E E E T 32 5 3 6 Indication of refrigeration state section thickness setting and counters rrsrsnerverrerrenvnrenne 33 o4 Eee AN EEE EE 34 5 4 1 Selecting a sectioning mode tart and stop SECTIONING rrrrrervarvrverrerrenrrverrerresrrnerserrerssvesvenne 35 5 4 2 Setting the sectioning WINdOW vssisssscsarsevaccdisaciivacvesacaninsansbivisbedenadinnsorsstastaessaneniniseasaavadiehantiindensenn 36 FN 37 DAA ENG 3 M EN nian E A N A E 38 5 4 6 Trimming and sectioning functions switching between trimming and sectioning
58. rect the loss of height by pressing the 4 button until the instrument is lifted back up to the desired working level 2 The cryostat Is in an inclined position due to an uneven upward movement of the lifting pistons Difference of level among the four legs gt 5mm Remedy Have the instrument travel once up and down over the entire height adjustment dis tance press 4 until the instrument reach es the upper limit position and then until the instrument reaches the lowest possible position Then lift the instrument back to reg ular working height Should the problem re occur several times call Leica Technical Service 3 The height adjustment device does not reach the maximum upper level or the instru ment lowers Itself by a relatively large dis tance gt 5 cm within a short time Remedy Call Leica Technical Service 9 1 8 Technical Data Nominal voltage 230 240 V 100 115 V Nominal frequency 50 Hz 60 Hz Type of actuation Elektrohydraulik Total height of stroke 230 mm Carrying power 200 kp Dimensions Width 880 mm without control unit 1030 mm with control unit Depth 555 mm Weigth approx 30 kg 61 9 92 Ordering information accessories 62 Knife holder base scccasusaiesanacavtinntsndbositsaraescanscinnatenbetersaniinnuntwaiaatanntts 14 0419 26140 Kite holder CN ecorimrennessmenaniani i 14 0419 33993 Knife SUD D 0 EE 14 0419 19426 Mer 14 0419 19427 Anti roll plate aSSy EEE 14 0
59. redefined number of sections in the Continu ous stroke sectioning mode Once the preselected number of sections has been carried out section counter reading 0 the specimen head will stop automatically in the lower limit position The reference value i e the value from which the preset counter counts downward can be selected in the corresponding menu Item In order to be able to set a reference value the preset counter function must be active lf the preset counter is deactivated see diagrams above press y to activate 30 Now there are two options 1 Increase or decrease the actual reference value by pressing 4 or 4 2 Press and y simultaneously in order to reload the former reference value 0 when setting up the instrument and then press ei ther 4 or individually in order to select the desired reference value Depending on where you start from and where you want to go method 1 or method 2 is quicker see ex amples Example 1 Actual preset counter reading 50 Former reference value 300 New reference value of 65 to be selected Method 1 is most convenient e Increase from 50 to 65 by pressing Example 2 Actual preset counter reading 50 Former reference value 300 New reference value of 320 to be selected Method 2 is most convenient e Press A and iy simultaneously to reload old reference value 300 e Increase from 300 to 320
60. rrsrnvrnvrrervenrrnrnverververvevenververrenerserverresrenerserversesssvesverversesenne 13 5 MENE 13 2 9 0 Motorized sectioning EE EEE EE 13 2 3 7 SEE 13 2 3 8 Frozen parts of the instrument and frozen accessories ceccessscssescsscsecssseeeeceesseseesessesensanenees 13 2 3 9 Infectious radioactive material esernerenerverrerrenvaververvenrenrrnenverrenreserververresessesverrersesrssenrerrenenne 14 2 3 10 Disinfection and cleaning siessisssnunciincsenvatinsiavedituevssntenusinsecndenisebstsiurenviaingsiviasimendesiacvetasienadvedsdrvaaeuts 14 2 3 11 Removing reinstalling the microtome ssesrervenranrnnvnverrenrenenververresesvenververrenssserverresseserversersesesvenne 15 2 3 12 Display message Dry microtome rereresvrnvnververrrnvrververrenesververresesnesverversesssserserresssservessessesesvenne 15 SEE LEG EEE EEE EEE 15 3 CU ee E E E E E E 16 EEE 16 3 1 1 General site requirements rummmvrvrervvrvrernemevmenenernneenimemenenenvnenenernnenisienen 16 Electrical ENN Penn 16 3 2 Unpacking and installation EE 16 s FG ennaa E E EIEN Ea EEGs 16 3 3 Standard delivery cssrescssainsinccsintincssantviansanpunsvinspnneessancnnanvinendiausnbiedeaabanndsveadbianeusersulessesdentecssatientaas 17 3 4 Installing inserting SEES wcstiacnscavteintirocigianietconeirsineusieintedtessiunetisovinnetenostousianiaemnineindiestinisadint 19 3 4 1 Installing the handwheel EE EE 19 3 4 2 Inserting the accessories EE EN 19 Me 20 35 Prio
61. s Oo hannene ar 2 3 3 Otherwise the instruments are not functional e Insert mains plug into wall outlet e Continue with chapter 5 1 Setting up the in strument 20 Instruction Manual V1 3 03 2008 4 Instrument Properties 41 Overview o omm l e LEICA 3050S i m T oO Leica CM3050 S Cryostat O C1 RON 10 11 12 13 14 15 16 17 18 19 20 21 22 Cryostat CM3050 S Control panel 1 Control panel 2 Storage shelf left Storage shelf right Rubber mat Mains switch Automatic cutout for instrument Automatic cutout for sectioning motor Footswitch port Quick freeze shelf Stationary heat extractor Mobile heat extractor Specimen disc Thermoblock optional Section waste tray Brush shelf Specimen head w o specimen cooling Specimen head with specimen cooling Option 90 Prism instruments with specimen cooling only Knife holder base Knife holder CE Knife holder CN Knife holder CS Footswitch with protective guard 21 4 Instrument Properties 22 Instruction Manual V1 3 03 2008 4 Instrument properties 42 Technical Data i Operating temperature range ambient temperature 18 C to 40 C All specifications related to tempe rature are valid only up to an ambient temperature of 22 C and relative air humidity of less than 60
62. se as described above and as per the instructions given in this manual Any other use of the instrument is considered improper 1 3 Qualification of personnel The Leica CM3050S may only be operated by trained laboratory personnel All laboratory personnel designated to operate the instrument must carefully read the present instruction manual prior to starting work with the instrument Instruction Manual V1 3 03 2008 A U A y 2 1 General information on instrument design and safe handling This instrument has been built and tested in ac cordance with the following safety regulations on electrical measuring control regulating and labo ratory devices For current information about applicable stand ards please refer to the CE declaration of con formity on our Internet site www histo solutions com In order to maintain this condition and to ensure safe operation the operator must observe the in structions and warnings contained in this instruc tion manual Leica CM 3050 S Cryostat 2 2 Integrated safety devices The instrument Is equipped with the following safety devices e Handwheel lock e Handwheel grip centering e Emergency stop function instruments with sectioning motor only e Knife holder equipped with knife guard The safety devices installed by the manufactu rer of the instrument only constitute the basis of accident prevention Mainly responsible for accident free operation is above a
63. sly press and release RUN STOP and RUN ENABLE or depress and release footswitch e To stop sectioning Press and release RUN STOP or RUN EN ABLE or depress and release the footswitch The specimen head stops upon reaching the lower limit position Sectioning mode 3 Single stroke e Simultaneously press and release RUN STOP and RUN ENABLE or depress and release footswitch e The instrument carries out one complete section retraction stroke and the specimen head stops upon reaching the lower limit po sition While sectioning Is in motion LED RUN is illu minated While the sectioning motor is deactivated LED STOP is illuminated Both LEDs are located in functional area C 35 5 Operation Functional areas A and C Instruments with sectioning motor only 5 4 2 Setting the sectioning window The term Sectioning window stands for a dif ferent slower speed setting in that part of the sectioning stroke where the specimen Is actual ly cut while the remaining sectioning stroke above and below the specimen as well as the retraction return stroke are carried out at a higher speed level time saving feature If a sectioning window Is set speed inside the window is as set on the control panel Outside the sectioning window the specimen head trav els at a proportionately higher speed i e Position lower edge of specimen approx 3 mm above the knife edge rotate hand wheel clockw
64. sories disinfect if applicable then dry thoroughly and reinstall GJTJ 1 J 9C JO TJ JG gt See chapters 7 1 7 5 mf jHjolm e f le Indication in instruments with specimen cooling Instruments without specimen cooling only CT centered is displayed 28 Instruction Manual V1 3 03 2008 5 Operation 5 3 4 Menu functions setting parameters of refrigeration time and preset counter Once the instrument has gone through the initial sequence of indications you can go on to the menu functions to select and or change instru ment parameters If display is locked g Press key until display background lights up Next press the menu key With each key stroke the following parameters will be displayed dis play sequence as listed 1 Specimen temperature in instruments with specimen cooling only 10 50 C 2 Cryochamber temperature 0 40 C 3 Selected number of sections preset counter ON OFF and setting selection from 1 to 999 4 Maximum specimen temperature ON OFF 5 Realtime in 1 minute steps 6 Defrost time starting time of automatic cryochamber defrosting in 15 minute steps 7 Duration of defrost cycle cryochamber defrosting 6 to 12 minutes duration Menu items 5 and 6 displayed on 24 hour clock Leica CM3050 S Cryostat a Specimen temperature instruments with specimen cooling only AN Press key until menu item
65. specimen via orienting screws 1 and 5 Retighten clamping lever 4 Instruction Manual V1 3 03 2008 5 Operation 5 5 6 Trimming sectioning Inserting the knife e Insert the knife disposable blade into the knife holder e Select appropriate clearance angle at knife holder settings between 4 and 6 are suit able for the majority of applications For details on how to insert and adjust the knives please refer to the separate instruction manual s for your knife holder s See also chapter 2 2 Integrated safety devices incl subchapter 2 2 4 Knife guard i Manual trimming e Adjust knife knife holder relative to speci men see Knife holder instruction manual e Unlock handwheel e Use motorized coarse feed to bring speci men close to knife see chapter 5 4 5 Coarse feed functions e Select desired section thickness see chap ter 5 4 7 Setting the section thickness e Move anti roll plate away from knife e Rotate handwheel to trim specimen down to desired sectioning plane Motorized trimming e Adjust knife knife holder relative to speci men see Knife holder instruction manual e Unlock handwheel e Center handwheel grip See chapter 2 2 2 Leica CM 30508 Cryostat e Use motorized coarse feed to bring speci men close to knife see chapter 5 4 5 Coarse feed functions e Move anti roll plate away from knife gt
66. ss if possible reduce specimen surface Use different part of knife or replace knife Use knife with a different pro file Readjust clearance angle set ting Condensation forms on anti roll plate and knife during cleaning Leica CM3050 S Cryostat Brush forceps cloth or other cleaning item too warm Accessories and cleaning material to be used pre cooled only Store on storage shelves inside the cryochamber 49 6 Troubleshooting applications tips Problem Causes Remedies Anti roll plate damaged after adjustment Anti roll plate protrudes too far beyond the knife edge Adjust ment was done in the direction of the knife Replace anti roll plate in future lift away from the knife when aligning Handle anti roll plate more carefully Thick thin sections Wrong temperature for the tissue to be sectioned Knife profile inappropriate for the specimen to be sectioned Ice build up on the knife back Handwheel rotation not uni form or inappropriate rotation speed Knife not clamped tightly enough Specimen disc not clamped correctly Embedding medium poured onto cold specimen disc spec imen comes off the disc once frozen Blunt knife Incorrect clearance angle Specimen dried out Select correct temperature Wait until correct temperature has been reached Use knife with different profile c or d or possible switch to disposable blade system Remove
67. tails of the method Please provide further detail here If no please indicate why not Such equipment must not be returned without the written agreement of Leica Microsystems Leica CM3050 S Cryostat 65 11 Decontamination Certificate master 3 Yes No The equipment has been prepared to ensure safe handling transportation Whenever possible please use the original transportation case box Important to avoid refusal of shipment Place one copy in the unit prior to packaging or hand it over to the service engineer Customer as sumes all responsibility for the immediate return shipment of articles sent to Leica without proper decontamination documentation lf you have any further question please call your local Leica organisation Leica Internal Use If applicable note corresponding Job and RAN RGA Number Job Sheet No SU Return Goods Authorisation BU Return Authorisation Number Signature Date Institute Department Name Adress Position eMail Phone Facsimile Seica MICROSYSTEMS Leica Biosystems Nussloch GmbH Heidelberger Str 17 19 69226 Nussloch Germany Phone 49 0 6224 143 0 Fax 49 0 6224 143 200 www leica microsystems com 66 Instruction Manual V1 3 03 2008 12 Information for the People s Republik of China Administrative Measures on the Control of Pollution Caused by Electronic Products HH HART Hazardous substances SITE EF i Cd B PBLE ee md ttt
68. tch not properly connected gt Check connection connect properly if applicable 47 6 Troubleshooting applications tips 6 2 Potential problems causes and remedies Problem Causes Remedies Frost on chamber walls and microtome Cryostat is exposed to draft open windows doors air conditioning Sliding window has been open for a long period of time ata very low chamber temperature Frost build up caused by breathing into the cryocham ber Eliminate draft sources or change place of installation of the cryostat Close sliding window Consider wearing a mask Sections smear Specimen not cold enough Knife and or anti roll plate not yet cold enough sections melt Select lower temperature Wait until knife and or anti roll plate have reached chamber temperature Sections splinter Sections not properly flattened Specimen too cold Static electricity draft Specimen not cold enough Large surface specimen Anti roll plate poorly adjusted Anti roll plate poorly aligned relative to knife edge Wrong clearance angle selected Knife blunt or damaged Select higher temperature Eliminate cause Select lower temperature Trim specimen parallel in crease section thickness Readjust anti roll plate Align correctly Set correct clearance angle Use different part of knife or replace knife Sections not properly flattened despite correct temperature
69. ter 3 Status indication function The actual status of selected parameters Is in dicated in the display screen Some of those parameters are displayed auto matically e g sectioning stroke phase coarse feed position handwheel locked Others can be displayed by pressing the arrow keys e g selected section thickness preset counter status etc See chapter 5 3 5 Status Indication and chap ter 5 3 6 Displaying state of refrigeration sec tion thickness and counters 21 5 Operation 5 3 3 Display indications when switching on Meaning of indication Operator activity Initialization phase 1 DALE BEE EE 8 8 8 8 8 8 8 8 8 8 88 8 8 8 8 Initialization phase 2 HOoooodoodobooobbobtl ufufafufafufufufafefajufufufujn Software version cm sjo sjo s Vis XX XIX pH gg Selected display language Select language via keys Sectioning motor speed level slow fast Select speed level via A keys Temperature prior to n mie 7 Temperature upon switching on gt 0 C no AAC OTS e WEIR Fla t u rfe Acknowledge indication by pressing once briefly CITI J 1 1 YO JOT DIR IY MPH IC IR 0 CME SCG Re ERROR If this indication is displayed Do not switch on ancar I ME a ee Fer ee ee tit Prior to switching on both microtome and ed press yr unt Dee i ground lights up accessories must be completely dry Remove microtome acces
70. the specimen head instruments with specimen cooling only servvervrvevenn 46 Leica CM3050S Cryostat 5 Table of contents 6 10 11 12 Troubleshooting applications tip nrnrnvrnrnvnvnnrnvnvenenvnvrnvnvnvnnenenernvnerernennnernenenernenn 47 6 1 Displayed error EEE EE 4 6 11 ME NNN Nr 4 6 2 Potential problems CAUSES and remedies srrserrerrenrrnernerververvsvesververresennerverrerrenssserverrersssennens 48 6 3 Reference chart for temperature settings in MINUS C muvreververvsvervrverveverrevesvesvsvesesverenne 52 Disinfection cleaning and maintenance r rrarrsrrarsavnnvnnennvnnvnnvnnennnnnesnesnennennesnennnn 53 1 1 Safety instructions on disinfection and cleaning r senvrnvrrenvenvrnvnverrervervnrerverrerrenenserversesresennen 53 1 2 Spray disinfection with Leica Cryofect n m memernemnevimenememvnenenenenenivieneneivnenen 53 7 3 Disinfection with conventional disinfectants reresvrovrerrerrrnrrverrerrervererververrenrrserverresresennen 54 EEE EE E 54 1 5 Removing reinstalling the MICrotome aiitenicesctianeieseaineerasur marina bnenensrmnstineatinnmandanel 55 1 5 1 Removing the microtome Lae 55 7 5 2 Disassembling the specimen head instruments w specimen cooling Only uu bb 1 5 3 Reinstalling the microtome sresrrnvnnervervenvnververrenvenesvervenrenssnesverrersssesvesversessenesvenversensssesvesrensssennens 56 15 Exchanging the fluorescent light lamp
71. tions carried out since the counter was last set to 0 All sections in sectioning and trim ming mode are counted 5 Preset counter GIT 3 0 C Jo T 3 5 C Setting preset counter to 0 Press oy key can also be pressed while the continuous sectioning mode is in motion in this case the preset counter is set to 0 and sectioning stops upon reaching the lower limit position After it has been set to 0 the preset counter Is inactive Reloading the former reference value Press and v simultaneously Reloading the former reference value is possi ble also while the continuous sectioning mode is in motion Upon pressing both arrow keys the instrument continues sectioning without interruption start ing to count down from the old reference value 33 5 Operation 5 4 Control panel 2 Instruments without sectioning motor Instruments with sectioning motor In instruments with section ing motor the control panel is equipped with three func tional areas A B and C see large diagram A Functional area A e Sectioning modes e Sectioning window e Sectioning speed e Emergency stop function In instruments without sec tloning motor control panel 2 only consists of functional area B see below 5 10 30 50 100 150 TRIM Functional area B Functional area B SECT e Coarse feed e Coarse feed Trimming e Trimming e Section thickness selection e Sect
72. ty instructions Prior to connecting the height adjust ment device to mains check to make sure that the nominal voltage and fre quency indications on the device name plate correspond to the actual mains voltage in your lab Do not move the height adjustment de vice with the Leica CM3050 S installed on the frame If the place of installation needs to be changed first take the Leica CM3050 S down from the height adjustment device and then move both devices separately Never place your feet underneath the U frame when lowering the height ad justment device Risk of injury Only authorized Leica technical serv ice engineers may carry out repairs on both the Leica CM3050 S and the height adjustment device This applies espe cially to all work carried out on the hy draulic system of the height adjustment device 59 9 Appendix 9 1 4 Installation e Open the shipping box and remove all packing material including the Sty rofoam pieces e Take the height adjustment device out of the box e Atthe place of installation place the U frame on the floor with the open side facing yourself Adjust the U frame parallel to the wall maintaining a distance of approx 10 cm between wall and U frame e Push the up button to lower the height adjustment device to the lowest possible position e n order to be able to move the instrument use a fork wrench and unscrew the rollers on which the Leica CM3050 S moves
73. utomatic defrost cycle per 24 hrs Duration 6 12 minutes Manual defrost cycle 690 W 3 280 g 5g refrigerant R 404A 0 6 I EMKARATE RL 22S ICI 43 C 0 K 2K 10 10 C to 50 C 3K Manual defrost cycle electric 320 W 3 185 g 5g refrigerant R 404A 0 41 alpha 22 Kyodo or RENISO E22 Fuchs 23 4 Instrument properties i Refrigerant and compressor oil to be replaced only by trained and authorized service 24 personnel CM3050S 3 CM3050S 6 CM3050S 7 Microtome c UL Rotary microtome 120V AC 10 208VAC 10 230 V AC 10 Section thickness setting 0 5 300 um 60 Hz 60 Hz 60 Hz Overall specimen feed 25 mm 1800 VA 1800 VA 1800 VA Vertical stroke 55 mm 35 A eff 30 eff 25 eff Specimen retraction 50 um Maximum specimen size 40 x 55 mm T15A T1 112A T1 T10A TI Specimen orientation 8 x y Z axis 2 2 2 Trimming 6 thickness settings 5 150 um Motorized coarse feed 1800 J s 1800 J s 1800 J s slow 500 um s fast 1 000 um s Fluorescent light lamp cryochamber illumination Sectioning motor Option Sectioning speed 50 Hz version Osram Dulux S 11 W 21 Slow min 0 1 mm s Light color LUMILUX hellweif max 100 mm s brilliant white V 210 mm s 60 Hz version Osram Dulux S 13 W 21 fast min 0 1 mm s Light color LUMILUX hellwei max 170 mm s brilliant white V 210 mm s Cryocabinet Dimensions Width w o handwheel 800 mm Width includin
74. vated with the footswitch unlocking Is not necessary As soon as the footswitch is released the red srop LED Is extinguished e To continue work select one of sectioning modes 1 3 and restart the sectioning motion See also chapter 2 2 3 Safety Emergency stop function for further information on the emer gency stop 37 5 Operation Functional area B all models 5 4 5 Coarse feed keys The four coarse feed keys in functional area B function as follows Coarse feed fast backward Travelling speed 1 000 um s e Press m key to activate Specimen head travels backward away from knife a until it reaches the rear limit position b until it is stopped by pressing one of the coarse feed keys to stop the function any of the four coarse feed keys can be pressed Coarse feed slow backward Travelling speed 500 um s e To start press and hold Specimen head travels backward away from knife as long as the key is held As long as the backward coarse feed both fast and slow is in motion the HOME LED is illuminated LED HOME located in key Coarse feed fast backward Upon reaching the rear limit position the HOME LED starts flashing and the display in control panel 1 reads HOME 38 1 Coarse feed fast forward Travelling speed 1 000 um s e To activate the function press and hold The specimen head travels forward to
75. very information on accessories Instrument properties e Overview e Technical data Operation e Controls e Setup daily use and shut down Trouble shooting and applications tips e Operating errors e Display error messages e Temperature reference chart Disinfection cleaning and maintenance Warranty and service Electrohydraulic height adjust ment Ordering information accessories EC Declaration of Conformity Decontamination Certificate Information for the People s Republik of China Leica CM 30508 Cryostat 1 1 Symbols used in this manual and their meaning A i Warnings appear in a grey box and are marked by a warning triangle AN Notes ie important user information appears in a grey box and is marked by an information i symbol 5 Figures in brackets refer to item num Fig 5 bers in drawings or to the drawings themselves Instrument type All information given in this instruction manual applies only to the instrument type indicated on the title page A name plate indicating the instrument serial number is attached to the back of the instru ment Required in feica N N Ce formation for all inquiries SOE oe 8 IDA For any inquir 120 V 60 Hz 1800 VA les specify e Instrument please Hovpikunlurdg Fulmenge 2809 tog Maximal zudsager Setnebscruck 2809 SF 3 Kollemitta R 404 A type Objektkihlung e Serial Katenita R 404
76. y damaged When removing the microtome e Wear gloves when removing the micro tome while it is still frozen gt Risk of frost bite e Qn instruments with specimen cooling do not distort the refrigerating tube If distorted it might break causing ex tremely cold refrigerantto escape gt Risk of frost bite Before reinstalling the microtome e Microtome must be completely dry gt Humidity in the interior of the micro tome freezes and causes microtome malfunctions and or damage to the microtome e All accessories tools removed from the cryochamber must be thoroughly dry be fore putting them back into the chamber gt Risk of icing Leica CM3050 S Cryostat If the error message Dry Microtome is dis played in control panel 1 the following has happened e Cryochamber refrigeration has been in terrupted for an extended period of time e g power failure causing the chamber temperature to rise into the positive digits e fthis message appears do not switch on the instrument but remove the microtome from the chamber disinfect if necessary and dry thoroughly before reinstalling it into the chamber see chapter 7 1 to 7 5 2 3 13 Maintenance Only technical service engineers authorized by Leica may access the internal compo nents of the instrument for service and re pair The fluorescent light lamp chamber illumi nation unless broken or splintered can be replaced by the user

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