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Service Manual - Frank`s Hospital Workshop

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1. e nnn r nnn rn rrr rn nn MITTIT 58 3 13 External Temperature Probe n rr nr nnn rn nn 58 3 14 Serial Port 59 EEN Leed 60 4 SERVICE PROCEDURES een 61 4 1 Replacing Probe Tip or Probe Assembly ner nnn n TT MMMMM M 63 4 2 Bottles mee 64 4 3 Chassis 65 4 4 Electronics Rack T MMMMMMe Me e 66 4 5 Photometer TTLMMMMMMMeeee Me e e e 68 4 6 Photometer Assembly Placement e ener rr nnn nnn rn TM MMMMMM M 71 4 7 Filter Wheel TMMMMMMee e e e V 72 4 8 Plate Carrier 76 4 9 Hydraulic System rer rur T rr rr rr Tr T T Tee nee ne nee 77 4 10 Valve and Pressure Pump een 80 4 11 Washer emt
2. en a Remove screws holding pho tometer cover Shaft Filter Wheel c Remove filter wheel belt and filter wheel Rotation is clock wise e Install neutral density filters dot screens and interference filters me JO b Remove screws holding pho tometer PCB SN eX Vas E d Remove filter with ZA blunt object One or two nylon washers as disassembled One nylon washer f Assemble washers and filter wheel to filter wheel shaft Figure 4 7 2b Photometer Filter Change 74 106 Service Manual ELISYS UNO AN Human Step 4 Turn the photometer over so the three screw heads are facing up Remove the 3 6 32 screws retaining the photometer cover Set the photometer cover aside Remove the 4 4 40 screws securing the photometer PCB Hang the PCB to the side Note Do not touch the components on the PCB especially the photo diodes and their windows Do not let the filters get dirty dusty or contaminated Note the orientation and position and in the case of filters the wavelength of anything removed Remove the drive belt from the filter wheel Using the blade screwdriver loosen the filter wheel shaft Make notes of orientation of parts and their positions Remove the wheel Remove the shaft taking care not to misplace the nylon washers Step 5 Refer to Figure 4 7 1 Locate the filter t0 be removed The filters are fixed by use of a
3. m rr rr nnn nnn nn MTM MMMMM MM 36 2 12 Syringe Pumps e enn rn rn nn nn nn rn rn nn nnnm nnn TIT 36 EEN Tee 36 2 14 Computer Connections mm 38 2 15 Computer Software Interface mn 39 2 16 Instrument Functions enee 40 3 1 Service Tools er nr rn nnn nn nn nn rn nn nnn nn Tr TT MIT MMMMM MMMM 41 3 2 Error Messages enn n rr nn rn nn nn rn nn nn nnn rn nn Tr Tnm MITTIT 51 3 3 Valves m nr nn nnn nn nn nn nn 53 3 4 Vacuum and Pressure Systems nn 53 3 5 Motor Control eren rn nn nn nn rn nn nn nn nnn nn nnn T TT MTTMMMMMMM 53 3 6 Incomplete Aspiration nn nr rn nr nnn nn nn 55 3 7 Incomplete or Inaccurate Dispense nn rrr rrr nnn MTM 55 3 8 Photometer ee nn nn rn nn nn nnn nn nn rn nn nnn nn nn rnm MITTIT 55 3 9 Plate Temperature Control n rn nn nnn nn 57 3 10 Coil Temperature Control o n rn rr nnn rn rn nn 58 3 11 Probe Temperature Control eon r nnn nnn rr rn rn nr nn T MTM 58 3 12 Carrier Temperature Control
4. Turn the lamps on Turn the lamps off Display the filter voltages of the 4 channels at all 8 wavelengths Read row 8 wells nn strip 1 12 pd filters n of readings Read the specified well x strip A H nn strip 1 12 pd filters n of readings Display the filter volts at well position x strip A H nn strip 1 12 Take and store an air reading Read and store blank values x strip A H nn strip 1 12 entered for Channel 2 right rear all 4 channels all 8 wavelengths refer to error 523 Displays current stored channel blanks Service Manual ELISYS UNO 49 106 3 1 20 Probe Macros IGOWLxnn IGOWDXNNVVVVSS ir IPLVLXxxx IG RAANNVWWWVSS neue IGR1DNnnu vu VSSs IGR2AnnNvyvvySS eee IGR2Dnnvvyy SS Go to the specified well x strip A H nn strip 1 12 Go to the specified well and dispense x strip A H nn strip 1 12 vvvv vol in uL s speed 0 9 Probe dispense level at plate height from well bottom to disp point Change Depth of well in relation to probe pppp new depth Max probe depth xxxx maximum depth over racks Go to the specified position in Rack 1 nn position number Go to the specified position in Rack 2 nn position number Go to the position in Rack 1 and aspirate nn position vvvv vol in uL s speed 0 9 Go to the position in Rack 1 and dispense nn position vvvv vol in uL s speed 0 9 Go
5. f j a White Cooped Screw j j Pull to remove the white corrugsted air cooling tube ot the gold face plete on the back panel Tube Coupler Figure 4 7 2 Photometer Diagram Step 1 Disconnect the instrument from the AC mains and detach the power cord Disconnect bottles Remove the right side cover Set the cover out of the way Step 2 Refer to figures 1 3 1 and 4 5 Locate the photometer Note the location of the red marking for cable orientation before removing connector Note the orientation of the harness cable and connector from the coprocessor board Unplug the two upper cables from the photometer junction PCB Leave all other connections on this PCB alone Unplug the ground wire at the red quick disconnect terminator located at the chassis end of the ground wire Leave the two individual RED wires going to the lamp assembly attached Remove the white corrugated air tube by pulling it from the gold colored tube coupler plate located on the instrument s back panel Step 3 Locate the white slotted cap screw retaining the photometer to its mount Turn the cap screw until you can remove the threaded shaft Remove the photometer by sliding it out the side NOTE DO NOT REMOVE THE RED LAMP WIRES from the photometer Removing or altering other parts of the photometer will require the unit be realigned Do not move the lamp or the lamp bracket Service Manual ELISYS UNO 73 106 ec
6. AWARENESS Pressure arrier y Sensor Carrier External Pressure acuum Plate Temp Temp Trimpot Trimpot Temp Figure 5 8 Trimpot Locations on Daughter Board Service Manual ELISYS UNO 87 106 6 Alignment 6 1 Photometer Plate Carrier Alignment of the photometer plate carrier is only required to verify if you suspect that your instrument is having trouble reading e g poor repeatability on reading or if you move the X home or Y home flags Alignment Check Remove the aerosol shield probe shield and lamp housing cover Lift the instrument hood Taking care not to disturb the lamps release the lamp bracket by removing the 8 32 retaining screw Rotate the lamp bracket 90 degrees clockwise and screw the lamp bracket back down This will keep the lamp bracket in a position out of the way allowing you to see the position of the plate carrier with respect to the photometer Place the metal carrier in the metal plate insert into the plate carrier and enter a read well command for position A1 e g RWELA01723B Look straight down at the holes in the channel block while the read command is occurring and the holes of the channel block should be centered in the holes of the plate insert Repeat with position H12 opposite sides of the plate command RWELH12723B Refer to section 3 1 9 Photometer Commands Alignment Procedure To compensate for backlash the plate carrier assembly is fitted with a set of index p
7. The probe empties and flushes in the wash cup Liquid exits the wash cup through tubing that drains into a waste trough underneath the rack mover From there liquid drains to the drain bottle by gravity The washer aspirates liquid from the plate into the waste bottle The wash trough drains by gravity into the Waste Trough 2 11 Bottle Fill Level Sensing The signal BOTLV is fed through a voltage divider to stainless steel wire probes mounted in the caps of the wash rinse and waste bottles The signals are time division multiplexed via U9 into V F converter U14 and the resulting frequency appears at the CLK2 input of 8254 timer U2 The microprocessor reads a change in frequency which corresponds to a change in conductivity of the material between the probes Refer to 1 3 3 and 1 3 15 2 12 Syringe Pumps The syringe pump is an OEM component of the ELISYS UNO consisting of two syringe drives and one valve drive which are capable of independent operation through its own microcontroller board The pump module has its own internal command set and communications between the syringe pump and the ELISYS UNO Main PCB occur serially via a 3 wire cable connected to J2 on the Main Board The valve has four possible positions that connect any two ports at right angles The valve is used in only two of the four positions allowing the 2 5mL syringe to connect to either the priming tube or the interconnect tube Priming fluid from the prime bottle is in
8. 5 5 Coil Block Plug thermistor probe comes with distributor pack in External Probe Sensor jack Insert thermistor in the hole front and center of the block Compare Ext with Coil Measurements should be within 37 C 0 2 C If not adjust coil temperature pot on the Probe Sensor and Heater Control PCA R23 See caution at the top of the section Keference vacuum gauge l6 in Hg From vacuum blue y To vacuum side fitting on rear panel of waste bottle Tee fitting Figure 5 6 Gauge Connections Vacuum Calibration Service Manual ELISYS UNO 85 106 5 6 Vacuum Attach a vacuum gauge to the blue fitting on the right side of ELISYS UNO Type VAON to turn vacuum on then type ISTAT Vacuum and gauge should agree If not adjust pot Daughter R9 until agreement See caution at the top of the section Reference pressure gauge 6 psi From pressure red To pressure side fitting onrear panel of wash and rirse bottles Figure 5 7 Gauge Connections Pressure Calibration 86 106 Service Manual ELISYS UNO AN Human 5 7 Pressure Attach a pressure gauge to the red fitting on the right side of ELISYS UNO Type PRON to turn on pressure and wait for pressure to be reached Pressure should be at 5psi If not adjust the pressure trimpot Daughter R10 until pressures agree See caution at the top of the section 5 8 Trimpots on Daughter PCA E E HNOLOGY aro Ka 17 ba IS 1
9. Home Processor Al ve Plate y Plate X One Shot Count Home Wash Home Plate Mover Junction LED D14 status indicator of the thermistor on the Plate heater LED D15 currently not in use LED Display Board e P voc 3 E E Se 9 SG o 3 TG e zo 92 E xx q SS Oo ke ken AULA BOBBBBCLU 66 oe vou 2262 5 EEE 9 2075 2 S60 AASS A TTI A 2 vd a ob AS 2 SE zb A 3 zc oo SG 22902 ac e A 2a 2 os SUS SELO Ox nas SG GO a 42 106 Service Manual ELISYS UNO AN Human 3 1 3 Reports The ELISYS UNO software includes a number of functions intended to make it easier to diagnose problems in the field For example there are several files and reports which the end user can send back to the service center At the top of every report printed by ELISYS UNO is a message in the form ssss v vv bbb xxxx yyyy ppp ddd where ssss INGICALES nennen Instrument serial number V VV INDICATES 0 0 eeeecceececeeeeeeeeteeeeeeeeeees Software version number bbb indicates A Software build number i See note below xxxx Indicates rreren Main firmware revision yyyy indicates oes eeeeeeeeeeeeeeeeeeeeeeeeeeeeees Coprocessor firmware revision ppp indicates ee ceeeeeeeeeeeeeeeeeeeeeteeeeteees Primary filter if the report is for a single assay ddd indicates o Differential filter if the report is for a single assay Note If a set of parenthesis with another v vv and bbb appear you are viewi
10. Probe x Mover uc m ee bk Dx X Kam Bracket ER mn e E E eee Yee Fate Mover ALL eie AG Assembly EE Doo o O x vave and Pres Pump SE E E De 7 EEE EE Bed FE as DO Do LL jpemetamp EEE Piste Carrier Cl ee 62 106 Service Manual ELISYS UNO AN Human 4 1 Replacing Probe Tip or Probe Assembly Replacing the Probe Tip Should the probe tip become bent or otherwise damaged it can be easily replaced Unscrew the tip fitting Remove the probe tip Add a small amount of ELISYS UNO lubricant to the tip of the brass connector on the probe body Smooth out the lubricant on the threads by rolling a finger around the brass connector Screw the probe tip and fitting onto the end of the probe assembly until it is finger tight Make sure that you cannot wiggle the probe tip up and down in the fitting after it is screwed on this is an indication that the probe tip fitting is not screwed on all the way Replacing the Probe Assembly In the event that the probe assembly supplied with the ELISYS UNO becomes damaged it may be replaced as follows Remove the probe tip fitting and probe tip Loosen the set screw retaining the probe in the heating block with the supplied hex key Unplug the probe s green 4 pin connector from the junction board Unscrew the stainless fitting making sure that the red o ring remains on the end of the fitting If the o ring has fallen off into the fitting hol
11. and associated logic Check the sensors Make sure the transistor and LED are aligned Rack 1 is jammed the motor stalled while the instrument was attempting to move the rack 1 Check for mechanical obstructions or broken belts Check motor driver U22 and associated logic Check the sensor Make sure the transistor and LED are aligned Rack 2 is jammed the motor stalled while the instrument was attempting to move the rack 2 Check for mechanical obstructions or broken belts Check motor driver U22 and associated logic Check the sensor Make sure the transistor and LED are aligned Diluter Not acknowledging check the cable that plugs in the back of the diluter Service Manual ELISYS UNO 51 106 Uu E CSIO Inactive unable to communicate with coprocessor OT Zee ee here Incorrect length for Move All command VOS EE Timeout waiting for coprocessor message BOM Assess seth Diluter is not responding QUITE Timeout waiting for completion of last coprocessor command ONG cad dde ees Check reagent sample level R Probe dp too large RAN E Probe sensor malfunction 019 icri ec E e EC ER ERES parameter checksum error PODO ssiss sas seta siso tasas tas aaa ts ossada casais dessas probe jammed while level detecting 50241 EEN small syringe stroke error 2022 M v mo mid Mm m mM Ne ME large syringe stroke error The Coprocessor error messages start at 500 an
12. 106 Service Manual ELISYS UNO Bottle Connections Waste Sensor Black Waste Black Rinse Rinse Green Sensor Green Wash Yellow Wash Vacuum Blue Sensor Yellow Pressure Red Figure 81 3 3 Right Side Panel Showing Bottle Set Connections Power Switch c Power Inlet External Protective Reset Grounding Terminal Figure 1 3 4 Rear Panel Serial Port Switch AN Human Service Manual ELISYS UNO 9 106 Plate X Mover Oo Undercarriage e Y Index ORB q Sensor Base Is Not Shown X Home Sensor Drive Timimg sea Ka d X Home Flag X Index X Count Sensor Sensor e 0 0 X Limit E Sensor Y Home Sensor Figure 1 3 5 10 106 Service Manual ELISYS UNO AN Human Plate Y Mover Opto Encoder Wheel Drive Screw Wash Trough Shuttle Plate Insert L IL Figure 1 3 6 Service Manual ELISYS UNO 11 106 Rack Y Mover Rack Junction PCA TU H Kl LA In Switch Reagent Rack Motor x rj Figure 1 3 7 12 106 Service Manual ELISYS UNO Human Probe X Mover Serial s 2900 1783 to current 1opeog 1PM Japoougd to 2900 1013 Es Tal e Josuoas E SununoJq uoneug fe 1osuog CN MS p Z 99014 ra wor ba E
13. Back of Electronics Rack See also System Overview Diagram in Appendix B ELISYS UNO uses two switching power supplies both of which are mounted in the lower portion of the electronics rack The power supplies should require no maintenance or adjustment in the course of normal operation To access the power supplies unplug the instrument and remove the four screws which attach the back cover of the instrument Service Manual ELISYS UNO 31 106 Facing the back of the instrument you will see a small box at the right side containing the power receptacle and the main power switch Note that ELISYS UNO has no voltage select switch as the power supplies are auto switching Inside this box are the main fuses for the instrument See Fuse Replacement for more information Two sets of AC lines originate from inside this box One set goes directly to the first power supply and the other goes to the relay board The first power supply converts AC power to provide DC 12V 5V and 24V to the instrument It is always operating when the instrument is turned on The DC output from the first power supply connects to the adjacent DC Junction Board using a 13 wire header This board distributes the DC voltage and has connections for the Main Coprocessor Daughter boards as well as the syringe pumps and internal lamps See Appendices for the layout Only one of J56 or J54 will be connected to the pumps and J10 24VDC powers the internal lamps The three c
14. UL and CSA standards or approved for the country of use Use only the recommended fuse Do not substitute any other rating Replace the AC cover Also check fuses on power supply boards Fuse 3AG 1 1 2 Amp 6 3mm X 32mm T Slow Blow Follow the reverse of the procedure above to replace the AC panel Power supply fuse replacement N Set the power switch to OFF o position before performing any service work on instrument WARNING Unplug the instrument and remove the four screws that attach the back panel to the unit Each of the two power supplies contains a fuse on the AC mains CAUTION The two power supplies do NOT use the same types of fuses make sure to install only the proper fuses e The first power supply larger of the two uses a size 0 or 3AG 32mm X 6 3mm 5 amp 250V quick blow e The second power supply smaller power supply board uses a size 5mm X 20mm 2 amp 250V quick blow 4 5 Photometer The instrument incorporates solid state electronics in a very reliable field proven design If you experience problems please contact the manufacturer photometer assembly amp chassis mount lamp cover box je lamp terminal blocks forced air ventilation aperture block sample plate channel block M SE ee photo detectors filter wheel belt pcb cover box mount to chassis attachment screws Figure 4 5 Photometer Diagram 68 106 Service Manual ELISYS UNO AN Hum
15. Windows 95 prior to 95 the system should be upgraded ELISYS UNO has been tested with Microsoft Windows 95B 95C 98 Windows NT 4 Workstation Windows NT 4 Server and Microsoft Windows 2000 Professional Beta 3 The Processor section will show information regarding the computer itself The MHz speed of the computer is not available under Windows 95 98 The Memory section will report the total amount of physical and virtual memory There should be at least 8MB 8096K of physical memory The total virtual Page memory should be at least twice the physical memory If it is not the user should change the virtual memory settings under the Windows Control Panel Service Manual ELISYS UNO 43 106 3 1 6 Using ELISYS UNO Pack ELISYS UNO Pack CWPack is a supplemental stand alone program designed to ease the transfer of various ELISYS UNO data files assays jobs racks data etc Because the functionality of ELISYS UNO Pack will eventually be integrated into the main ELISYS UNO program CWPack has been kept intentionally simple and inflexible Contact your dealer if you do not have CWPack on your computer There are two basic operations that CWPack can perform packing and unpacking Packing files To pack a group of files perform the following steps Using the File Management functions within ELISYS UNO export all files to be transferred to an empty directory Start CWPack by selecting on the Windows Start Menu Prog
16. X and Z Movers are combined and are responsible for the probe movement Figure Number Figure 1 3 1 Figure 1 3 2 Figure 1 3 3 Figure 1 3 4 Figure 1 3 5 Figure 1 3 6 Figure 1 3 7 Figure 1 3 8 Figure 1 3 9 6 106 Service Manual ELISYS UNO Human ELISYS UNO Component Continued Figure Number Washer Figure 1 3 10 NOTE Responsible for the aspiration and dispensing of liquids using the wash head Electronics Rack Figure 1 3 11 NOTE Holds the main boards PCAs and the power circuitry Pressure and Valve Bracket Figure 1 3 12 NOTE Controls the fluid flow of the washer Syringe Pump Figure 1 3 13 NOTE Controls the fluid flow of the probe Photometer Figure 1 3 14 NOTE Houses the lamps filters and is responsible for photometric readings Bottles Figure 1 3 15 NOTE Holds liquids for the washer system ELISYS UNO Coordinate System Figure 1 3 16 NOTE Explains the coordinate system for ELISYS UNO Service Manual ELISYS UNO 7 106 Wash Station Photometer Wash Rinse and Waste bottles Rack 1 Rack 2 Reactlon Plate Reagent Rack Sample Rack Figure 1 3 1 Instrument Exterior without aerosol shield Probe Shield Syringes Top Cover or Hood z r Ze ra D EN D a Right Side Prime Panel Reagent Rack Sample Aerosol Rack Shield Plate Carrier Figure 1 3 2 Instrument Exterior aerosol shield translucent and syringe tubing not shown 8
17. air gap in microtiters that is aspirated following a pickup luL is the default and should always be sufficient Dispensetiob The is a height in counts relative to the bottom of the well This specifies that position at which the instrument will dispense when Dispense High is specified in the sample protocol The default is 42 DispenseLow sssssseeeeee The is a height in counts relative to the bottom of the well This specifies that position at which the instrument will dispense when Dispense Low is specified in the sample protocol The default is 35 LevelDetectOffset lt lt The level detect offset is used by the software to account for the difference between the detected liquid level and the actual liquid level The default is 0 and should not be changed MinSampleDispense This is the minimum amount that will be dispensed including push volume The default is 5uL MixExtraTime This setting is no longer used MixSpeed A This setting is no longer used PDrb ZAepiratet achor eere When aspirating from a bottle or vial the software calculates the ideal distance to move into the vial to aspirate the specified volume This factor is applied to calculation to account to variation in vial sizes and wall thickness The default is 1 35 35 PrbZMinimumDip sseeesse This is the minimum amount i
18. arrows move a large distance single arrows will move a smaller distance Service Manual ELISYS UNO 89 106 red arrows will move the rack in and out of the instrument blue arrows move the probe left and right green arrows move the probe up and down When you move the plate or the probe the instrument automatically raises the probe To lower it to the bottom of the well again use the button showing the probe being lowered Click Done to save the new setting or Cancel to keep the old one 6 4 Wash Cup Aligning the wash cup Select Align Wash Cup from the Instrument Setup dialog The probe will move to the center wash position and lower into the cup The probe tip should be roughly centered in the small center wash cup with the tip beneath the surface of the water in the cup Use the blue arrow buttons to move the probe left and right Use the green buttons to move the probe up and down The double arrow will move a larger distance and the single arrow a smaller distance When you move the probe left to right the probe will be automatically raised To lower the probe into the wash cup use the button showing the probe being lowered Click Done to save new setting or Cancel to keep the old one 90 106 Service Manual ELISYS UNO Human 6 5 Washer Aligning the washer Select Align Washer from the Instrument Setup dialog Insert a plate or strip tray when prompted and select OK T
19. e Qr Jopeag sde POU Abou o Z m x EEE s IOJOIN vod E NLONT XHd n asus ARE Ze S Zununow uongqi e 10suas Z 94014 d wo bg a Figure 1 3 8 13 106 Service Manual ELISYS UNO Probe Z Mover Figure 1 3 9 14 106 Service Manual ELISYS UNO Human Washer Dispense Aspirate Guide Rods Figure 81 3 10 Service Manual ELISYS UNO 15 106 Electronics Rack d Proto Racka Cow See Dis Za 8 Wise Power Fan Phatemeter Junction Junction Probe X sg v wi Doan Power Pump Mo ES r 1 Home Photometer Calibration Bio Back Chanel 1 valves Duni pe SIEH oe e DAUGHTER MAIN Chanel 3 Colitention Processor 1s gl Law End irl Sewer Chenvel 4 Shot Exteme Tero Sensor Wester Carrier Piste Tomo Prata xpos Plate Y Tanp eg 6 COPROCESSOR Fie G Poner Home s Vacuum Ni Probe Z Prote X wig Supply Piste V Tete Y Junction Junction Power Count em Evternl S LED Temperature acre EE zen Flug Dr Red iron Stripe D J Sires Mw Z Figure 81 3 11 Service Manual ELISYS UNO 16 106 AN Human Bottle Connections Pump and Valve Bracket WASH FLUID RINSE FLUID PRESSURE ul gt EI E gt a adi ud an gt x _ z ut E 2 Se E A 2 c ca BE Es ZE o 8 N eSI tus S S E B a us wa x ze a s va ul 8 so gt amp GE 3 om a KK PII gt a un RINSE YALYE
20. into the 8254 U10 on pin 9 where is read by the software The temperature is calibrated via the trimpot R23 on the probe sense board See the section on calibration The drive to the coil heat is provided by one gate of the L293 driver chip on the probe Z junction board which in turn drives the 4 75ohm heat resistors on the underside of probe sense board The LED D11 on the probe sense board indicates that the resistors are currently heating To check the current temperature setting use the PARM command and locate the Coil parameter 0370 37C To check the current coil temperature send a ISTAT command The Telix Mode window will display the coil temperature in the top left status box To turn the coil temperature control on use the command COON and to turn it off send COOF 3 11 Probe Temperature Control The probe body itself is also heated but uses a self regulating circuit rather than being under software control The thermistor inside the probe is fed into the comparator made up primarily of U29A on the probe sense board The circuit is calibrated via the trimpot R20 The output of this comparator switches one of the L293B gates on the probe Z junction board This driver provides power to the 2 32ohm 1 8 watt resistors inside the probe Heating is indicated by the LED D8 on probe sense board The firmware is able to turn off the coil temperature control by disabling the L293B chip U27 Probe temperature control is on when the
21. limited memory Try setting your print driver under Printers to print black amp white lower the print resolution or upgrade your printer driver 60 106 Service Manual ELISYS UNO Human 4 Service Procedures WARNING Set the power switch to OFF 0 position before performing any service work on this instrument ES JE Cover Lift Hood Probe Shield Aerosol Shield Left Side Panel Work On LL ae Boma EEE ER EES EE ES SyingePump x Is x Washer x o x x J x Racky Maer x Block ox px x Probe Z Mover x J x x Probe xZ Mover x x x Probe X Mover x III Photometer x x J x Filter Wheel Lamp Bracket x A x j Plate X Mover oo x j x Plate Y Mover x x PlateMover d o x 2 x Any Main Board x x J 1 x IAC Assembly x x o y Electronic Rack x x J A0 j x Valve and Pres Pump x x x x ee a ET EE E Wash II EECHER Bleed Tx Is j x E Pressure Ctl x x x Waste Trough 1 Wash Cup Dome tamp 1 1 4 14x 1x Plate Carrier x x ass 0 14 4 Service Manual ELISYS UNO 61 106 I Right Side Panel Plate Cover Photo Holder Release Pres Remove Bottes SES ERR O 7 OC Battles TT Syringe Pump eh DO DL o o Ricky Mover TD TE Do o Probe ZMover E E
22. mentioned steps Extra tie wraps of the type used for routing the cable have been enclosed in the ELISYS UNO service parts pack The cable should be routed making sure to have a small service loop of about 6 7 for plate travel Check the plate carrier s temperature calibration as per the instructions given in 5 4 Calibration Plate Carrier When installed go to Alignment Plate Carrier 76 106 Service Manual ELISYS UNO Human 4 9 Hydraulic System FEEDER LINE se LI LF Ze COUPLINGS AN SILICONE COUPLING SLEEVE T CONNECTOR VALVE A EEN m A gt L TE PRIMING TUBE SH HR ees ol U mI 2 By Geh E e a JE my L YHA CT Oo SYRINGE HEATER BLOCK INTERCONNECT TUBE A PROBE RIR 50 ul 25 mt M PRIMING FLUIO OOTTLE Figure 4 9 Hydraulic System Diagram 4 9 1 Syringe Pump To replace the syringe pump Unscrew the feeder line fitting from the T connector Remove the ELISYS UNO prime bottle amp prime tube by unscrewing the fitting to the valve and set the prime bottle aside Remove the four 6 32 truss head screws retaining the syringe pump assembly See Figure 4 9 2 Making sure the power to the ELISYS UNO is OFF pull the syringe pump assembly panel forward In the back of the pump assembly unplug the blue ribbon cable connector and the yellow power connector The syringe pump module c
23. pressure and vacuum ISPRSROX ante te tectus e dues Set the pressure control point xxxx PSI ex 0040 4 0psi IVOADOOOC uiia erm te e e mds Set or display volume calibration xxxx volume in mL ISSEN 42 22 2204 Show sensor levels Wash xxxx Waste xxxx Rinse xxxx xxxx counts 48 106 Service Manual ELISYS UNO 3 1 18 Diluter Control IWPRBWW eene emen IDASPWWV SX ea IDDSPvvvvSx EE IDMAPVVVVSX 0 ccccccceeececceececeeeeeseeeeneeeeenenes ISAIR OOK SX cs os ev eave Le Le t 3 1 19 Photometer IRbRCOWAnnpdnb nennen IRWELxnnpdnB eseeneeennnennenenn IVWELX N anna AN Human Wash probe tip vvvv volume in uL 2000 2 0mL if vvvv is not specified the default is used Specifies the default probe flush volume See IPARM and WPRB Initialize the syringe pumps x number of times Prime the syringe pumps n number of times to prime Scale factor for small syringe ie 1000 gt multiple specified volume by 1 000 Aspirate into syringe vvvv volume in uL large or small chosen automatically x syringe speed Dispense from the syringe vvvv volume in uL x syringe speed Make immediate air pocket vvvv volume in uL x syringe speed Set air pocket size xxxx volume x syringe speed air pocket is added automatically after each wash probe Display the available wavelengths Set the absorbance calibration factor Show the absorbance calibration factor
24. pressure or vacuum as necessary Two electromechanical valves the pressure control valve and the bleed valve are used to control and maintain the pressure in the system s wash and rinse bottles The pressure control valve controlled by U3 is an intermittent duty valve which regulates the pressure in the wash and rinse bottle When energized the valve closes causing pump pressure flow to be diverted from ambient into the check valve and into the bottles and sensor The bleed valve allows the instrument to relieve pressure during standby and when power is removed The bleed valve is a continuous duty valve also controlled via U3 and is continuously closed during any pressure cycle It also serves as an emergency pressure relief valve in the event of power failure or microprocessor crash and opens when pressure is no longer required such as in standby or when timed out A check valve oriented to allow flow to the bottle side only assures that the pressure is maintained on the bottle side of the system Pressure is sensed by the circuit of P2 and U8 Pressure build up occurs in the wash and rinse bottles simultaneously producing positive wash and rinse fluid pressure in the wash and rinse lines of the system see Appendix E Two intermittent duty valves called the Rinse Valve and Wash Valve which are normally closed are pulsed open via U3 on the Daughter Board The valves are used to regulate the flow of rinse and wash fluid to the washer h
25. reduce lamp life Insert the lamp leads into the connector until they bottom out The lamp lens end must be centered on the aperture and the lamp body must be perpendicular to the horizontal While holding the lamp in alignment tighten the lamp terminal Screws e Set the power switch to ON Shield your eyes from the lamp beam and observe the projection of the beam onto the aperture Refer to Figure 4 5 2b Spot Alignment The spot should be small and centered on the aperture The spot should be sufficiently large to just encircle the aperture If the spot is not centered repeat step 3 Service Manual ELISYS UNO 69 106 Select test IRFLT The instrument reports the detected voltage for each filter position All voltages should be between 2 00 volts and 10 00 volts If all the voltages report low repeat step 3 until optimum lamp position is obtained Lamp Lamp Terminal Connector Screws Lamp PCA Lamp must be perpendicular to strip Target d Ze E bracket and projected spot must be Paper Channel centered on aperture Block Projected spot must be bright and slightly larger than aperture hole so the outside edge of the spot does not touch or enter into the hole The spot should be well defined and centered on aper ture Use a small piece of paper as a target to observe the spot Figure 4 5 2b Spot Alignment on Channel Block 70 106 Service Manual ELISYS UNO AN Human 4 5 3 Adjust Lamp Bracket Level Screws
26. retaining ring Use a pointed object to get under the tabs of the retaining ring Pry two or three of the tabs teeth away from the filter until the ring is dislodged and remove the ring and filter The filter may be bumped or pushed from the wheel using the eraser end of a pencil or other blunt object Remove any remaining filter components from the filter cavity Locate then install the neutral filters and or screens if any that were included with the replacement filter some wavelengths do not require these extra screens and place them into the wheel cavity first Next insert the filter with the flat ring side down Place the replacement retainer ring over the filter so the tabs are angled away from the filter up and press ring in place with a 7 16 inch or 11mm wooden dowel or similar tool Step 6 Installation of the photometer is the reverse of disassembly Reinstall the filter wheel on the bracket taking care not to pinch any washer under the shaft Make sure no molding dimples from the washers are against the filter wheel Check the lube to see if it is dry dirty or missing Add more lube if needed Tighten the shaft Be sure that the belt is centered on the filter wheel and is not twisted Install the photometer PCB taking care to center the large hole around the shaft Note Replace the optical cover taking care to fit and position the gray cable in the slot of the cover Do not pinch the cable under the cover or it will be cut off Inse
27. strip 1 12 vvv vol in uL Wash row nn vvv volin uL x number of cycles Set current bottle to Wash Performs automatic prime Set current bottle to Rinse Performs automatic prime Show current bottle setting wash or rinse Check bottles levels Will report if there is an error Check wash head aspiration must change 0 5 open versus closed system Disable timed homing Enable timed homing Complete reset of the instrument Error messages are displayed when the instrument fails to operate correctly They are intended to help the operator locate the problem If error messages appear frequently a hardware problem is usually indicated Errors reported from Main processor go up to 499 Unknown command Check command for spelling and validity Parameter exceeds allowed range Too few or wrong parameter s Command has not been implemented Fluid has not been detected in programmed range Probe Z axis is jammed or Probe Z is jammed The motor stalled while the instrument was attempting to move the probe in the Z direction Check for mechanical obstructions or broken belts Check motor driver U27 and associated logic Check the sensors Make sure the transistor and LED are aligned Probe X axis is jammed or Probe X is jammed the motor stalled while the instrument was attempting to move the probe in the X direction Check for mechanical obstructions or broken belts Check motor driver U23
28. supplies ELISYS UNO contains three sets of the fuses The main fuses are located inside the AC box which is part of the electronics rack There is also one fuse in each of the two power supplies Since the supplies are autoswitching and can accommodate normal voltages it is unusual that any of these fuses should require replacement Replacing the main AC fuses N Set the power switch to OFF o position before performing any service work on instrument WARNING After unplugging the instrument locate the AC panel on the back of the unit The panel contains the power receptacle and the power switch Remove the two screws one at the top of the panel and one at the bottom that attach this panel to the instrument Gently pull this panel away from the instrument there will be several wires attached Remove the four screws holding the black AC cover on the AC panel and remove the cover Service Manual ELISYS UNO 67 106 The double clip fuse holder is mounted on the inside of the rear panel Locate the blown fuse and remove it with a fuse puller or carefully pry it out with a small screwdriver Install anew 1 5A T rating 250 V cartridge fuse The fuse link is in a clear glass package and the element is spirally wound on a fiberglass core The fuses must be replaced by a 1 1 4 inch glass cartridge fuse commonly known as 3AG or size 0 The nominal dimensions are 1 25 X 25 inches 32 X 6 3 mm The fuses must be approved to
29. the mechanisms and other systems The firmware is actually divided into two parts one for each of the two Z180 processors inside ELISYS UNO The communication between the processors is described above The firmware is programmed into two EPROMs UV Erasable Programmable Read Only Memory and installed on the two microprocessor boards The firmware Main is for the Main board 2900 100 and Cop for the Coprocessor 2900 200 The firmware revision will be printed on the EPROM labels 30 106 Service Manual ELISYS UNO Human The software refers to the Microsoft Windows program running on the external PC This is the program that interacts with the user and controls the ELISYS UNO Fundamentally this software breaks down all operations into a set of commands which the instrument can execute For an explanation of these commands refer to the Section on Troubleshooting The version of the software can be identified by selecting Help About from the ELISYS UNO pull down menu In this dialog you will see a message in the format Version x xx Build yyy The Build Number is the primary means of identifying the software version Also see section 3 1 3 Reports 2 7 Power Supply ZN The power supplies contain high voltage and can cause injury Do not operate the instrument with the back cover removed WARNING Relay Power Power PCA Supply 1 Supply 2 AC Assembly DC Junction PCA Figure 2 7
30. the tube The flare should be concentric with respect to the tubing ID and have an outer diameter of 0 15 3 81 mm e Pull the fitting washer o ring combination forward to the flare the tubing replacement is completed e Screw the fittings back into place and route the tubing through the tubing clips Prime the system making sure all air is cleared and there are no leaks Service Manual ELISYS UNO 79 106 4 10 Valve and Pressure Pump Anatomy Rinse Wash Pressure Bleed Valve Valve Valve Valve Figure 4 10 Valve and Pressure Diagram 4 10 1 Valve Tubing Replacement The silicone tubing used in the valves may become clogged or worn with age If this occurs the tubing should be unstuck cleaned or replaced Try holding the valve open and pull the tubing to reposition the clamping to another section of tubing To replace tubing Set the power switch to OFF O Refer to Figure 44 10 Locate the valve to be serviced Note the relevant end connections and location of the tubing within the valve Refer to Figure 4 10 1a and 4 10 1b Pull back the pinch bracket and remove the valve tubing from the valve body Disconnect the valve tubing from the fittings at both ends 80 106 Service Manual ELISYS UNO Human Cutthe replacement tubing to match the length of the original e Install the replacement tubing to the valve body Push the tubing over the fittings until seated If you have trouble sliding the tubing o
31. 1 and D2 provide visual feedback to the service technician of the tach pulses and home positions The Rack Junction PCB holds the motor drivers The index pulse buffers and limit logic are on the Main PCB The rack junction connects to Main via 10 pin ribbon cable and two wire power cable Plate Two bidirectional DC motors under microprocessor control turn 3 8 16 machined stainless steel lead screws via a 55 tooth 40 DP timing belt and two 27 tooth 40 DP timing pulleys One motor is dedicated to X motion of the plate carrier the other to the Y motion of the plate carrier Each drive pulley attached to the motor has an encoder wheel with ten holes which create pulses when passing between an IR LED and a phototransistor providing tachometer pulses to the microprocessor at the CLK1 input and CLK2 input at 8254 U16 Phototransistors positioned at extremes of the plate carrier movements disable the motor drivers U7 and U11 via NAND gates U10 and U18 when the travel limits are reached Two of these phototransistors additionally indicate the home positions to the microprocessor LED D1 on the Daughter board provides visual feedback to the service technician of the tach pulses and home positions The plate mover junction PCB holds the motor drivers The Daughter PCB holds the index pulse buffers limit logic and connects to the main PCB via a 26 pin ribbon cable and power Additionally phototransistors generate X and Y axis index pulses to position the p
32. 2 2 380 d Fr Probe Heat Probe Heat vepir 08N02 lt lei Sense Top Sense N008 0052 f Bottom 296N pcb Thu Jan 19 15 09 35 2006 98 106 Service Manual ELISYS UNO AN Human sus Er d oC SE at mr Jur PCB LED 995336 00 va EC a PCB Photometer LED 995323 PCB Lamp 995322 PCB Photometer Junction 995324 Service Manual ELISYS UNO 99 106 Plate Mover Junction 995331 Relay Junction Board 995334 100 106 Service Manual ELISYS UNO Human ing Internal Plumbi Appendix E TOU 1N09 34NSS34d lN3IB8WV lt QV3H HSYM HONOYL 31SYM 17 AVAL dida 03719 HE 3SN3dSIQ JISNIY 3SN3dSIQ HSVM QY3H uiui dm HSVM dnd HSVM 38034 3 1033N dl 390 SCH MII 13AIMS 319NY Lues I i d AS 31SVM ual 2ISOHdONQAH WANIYA fl om 3ISVM 7 Zeng et AWM 3NO T or 3sNni HSVIM FUNSSIAd cima HSVM Nid 39NISAS JONIHAS zunssaua IVA ros qu s z 100 RA 3A1VA VS INI ef NOLLISOd 2 WINILSAS TOY1NO9 IIINVYAAH 0062 101 106 Service Manual ELISYS UNO YOSN3S 3UNSSIAI YOSN3S IVA ALNA LNV LLINH3INI I N ATOA ALNG INS LLINH3INI ON 3A1VA u3ongduy ONILHS Qv3H1Ng 301 ALNG SNONNILNOI O N 3A1VA ALNA SNONNILNOD ON 3A1VA 20 80193NNOO 331 res 3ATIV 393H2 dnd TIVWS 801293NNOO 331 311108 ONISN L 3NOOlTIS ONISNL w b 31SVM 333 AUAVYS WO LLOS NO ONILL
33. 22222 nen e enn een nee n nen eee 91 APPENDIX A SELECTED PART ILLUSTRATIONS 92 APPENDIX B SYSTEM DIAGRAM 95 APPENDIX C BLOCK DIAGRAM 96 APPENDIX D PCB LAYOUTS mi 97 APPENDIX E INTERNAL PLUMBING 101 APPENDIX F SCHEMATICS rere nn nnnnnnne 103 AN Human 1 Introduction 1 1 ELISYS UNO General Description ELISYS UNO is a computer controlled instrument system capable of automating any or all stages of assay processing that involve these functions fluid handling aspirating and dispensing from 2 uL to 2 5 mL incubating heating to 25 C or 37 C probe coil to 37 C or ambient and reaction plate to 25 C 37 C or ambient Mixing reaction plate only strip washing 8 wells simultaneously timing from 1 sec to 24 hours optical reading UV Visible range calculating using numerous preprogrammed equations data storage unlimited capacity reporting with many options and customization to choose from The system allows you to define and program an unlimited number of customi
34. 2900 HYDRAULIC CONTROL BRACKET Pressure Figure 1 3 12 Service Manual ELISYS UNO 17 106 Syringe Pump 5 GA FEED LI TUB e ae NGE fi 3 Ww HT Ha n8 L d c3 E Log ete e AGE o T3 AA LL ru aa Fail D Fo HP LU 4 E 1 e uj py amp a n k e e g O Hi O ULE RETAINING ge Figure 1 3 13 Photometer lamp cover box 4 lamp terminal blocks de m forced air e lamps ventilation hose d nal UEM diffusers Se re aperture block sample plate channel block filter wheel motor belt mounting supports filters filter wheel photometer to mount retaining screw mount to chassis attachment screws Figure 1 3 14 18 106 Service Manual ELISYS UNO Human Bottle Set Sensor Cables Locking Nut Hydrophobic Tubing Filter Figure 1 3 15 Service Manual ELISYS UNO 19 106 1 4 Technical Specifications Technical Specifications for ELISYS UNO Overall Dimensions 86cm W x 51cm L x 40cm H approx weight 45kg Capabilities dilutions predilutions dispensing single or multiple reagents Pumps two syringe pumps sized 50 uL and 2 5 mL Probe 316 stainless for maximum reagent compatibility level sensing Minimum and maximum 2 uL 1 95 mL volume Precision 196 Maximum number of 96 specimens including calibrators and controls Maximum number of reagents 40 assorted repl
35. 83 BLO ALIBRA IONA AAA A 85 5 1 Setup Aaa 85 5 2 Photometer TMMMMMee ee eM 85 5 3 External Sensor L L L 85 5 4 Plate Carrier L 85 5 5 Coil Block L L 85 5 6 Vacuum LTLLMMMMMeEMeeMeLe0 86 5 7 Pressure T LMMMMMMMMe eeyee eM 87 5 8 Trimpots on Daughter PCA caca nn 87 6 ALIGNMENT LLL LLL LLL LLL aiaa 88 6 1 Photometer Plate Carrier 88 6 2 Rack 1 Rack2 89 6 3 Dispense nn 89 6 4 Wash Cup nn 90 6 5 Washer 222 22222222
36. 94 106 Service Manual ELISYS UNO Human iagram System Di Appendix B HE 73411 36084 1V3H 3804d 1v3H 7102 NO LSINNIHL AE A 39084 NI II w03 Sipal NIV 001 0062 RA KO duras Dag MX Sit 3N010Hd MALINO LOM Siit NOILONNT DA 00 Doug ow al O roc vel 031 O10Hd CT 19Lr81 MOES 32903402 002 0062 LELBI wm YILHINYO l DO DO6Z H A9010NH33 SS3N3EVMY ener cmo mul Appendix B System Diagram 95 106 32w3831NI B3indMO3 M N3MOd OY Service Manual ELISYS UNO iagram Block Di Appendix C UNUE 39v L DLNVI SINddAS 43M0d SHIHILIMGE Q32IN90234 goa Migas N uidi D MH ATX nem Gow wa 17777 1 amas as 311404009404 api 2 zn 354 u1H9nvd i 1 i 1 i aM 403530440 i 1 H i 804 KII 434061 3418 H i d MW Teen aries y eSEE Y vm En er sai WIRE au boom op gas Service Manual ELISYS UNO 96 106 Appendix D PCB LAYOUTS Probe X Encoder PCB Probe X Junction Main 2 R Im RS DR STE z uii Pe 55 EN IT 29iN pcb Thu Jon 26 14 17 08 2006 D proposes Pn d mel 1111 mu 4212014241440424 010110 pas VEFETETEETETETETEY L E EE Optos Rack Junction a PEPSEESEY ele um Ke Bra Sech Ea i fimm E Service Manual ELISYS UNO XX62 0840
37. Enter the name of the file to be unpacked here Press the button labeled Start CWPack will now process the packed file and put the files into the directory you specified When it is done the status window will report Unpack Successfull You may use the File Management functions within ELISYS UNO to import the data files Additionally once CWPack has been run at least once you can double click on a PCK file from within Windows Explorer and CWPack will start automatically with the Unpack files option already 44 106 Service Manual ELISYS UNO Human checked and the name of the PCK file already in the Package File Name entry In the ELISYS UNO Export Directory box enter the directory name of your choice 3 1 7 Instrument Report With the computer connected to the instrument and the instrument turned on select Instrument Setup from the Setup menu of ELISYS UNO Once the software has gathered the necessary information from the instrument the Print Report button will be enabled This report displays several vital statistics regarding the instrument and the configuration stored in the computer For an explanation of these functions see the following Communications ComPoft 32324232 B es the name of the port ELISYS UNO is using Usually COM1 or COM2 CombsSpeed uti aceite should always be 19200 Handshaking een should always be 1 Settings A FPIUQ sis This is the size of the
38. H sans Ha uunc toes Move probe Z axis to home IPRXIMDOOQU siete nie eer ees Move probe X to coordinate xxxx IPRZMZZZZ eret AO Move probe Z to coordinate zzzz IPRBMXXXXZZZ ur Move probe X amp Z to coordinates Ud u u WAA T Find fluid level on rack sense liguid level IPARK RE Move probe to waste area of wash cup IPSENDOOQ ee Set fluid sensitivity xxxx sensitivity level IPC use Show probe counts 3 1 15 Temperature Control COON dcdit din dites Turn on probe coil temperature control STEE E Naa Turn off probe coil temperature control LO aa n EPEE Set the coil temperature control point tttt temperature ex 0370 37 C ITCON lH Turn the plate temperature control on ITOFE iic EO DO ERES Turn the plate temp control off IPEA Lancet tienden Set the plate temperature control point tttt temperature ex 0370 37 C Ee e Check if the plate temperature is 37 C 0 1 IPTON TR Show external temperature probe MPO RER AR Transmit xxx seconds of readings from thermistors 3 1 16 Rack Control IRKA RE Move rack 1 left to home IRK2H EE ERE Hus Move rack 2 right to home IKI Mun Move rack 1 to coordinate yyyy IRK2MYYYY ii Move rack 2 to coordinate yyyy IRKSMXXXXYYYY us Move both racks at the same time xxxx coord for R1 yyyy coord for R2 3 1 17 Pressure Vacuum and Valve Control EK Eeer Pressure system on IVAON RA Vacuum system on EE EE Vacuum system off IST BY us Standby Turns off both
39. IS 13AIMS 319Nv LHOIN L Gem NIVYO do 31SVM 9401431443 Service Manual ELISYS UNO 102 106 Human Appendix F Schematics Revision N Revision L Revision j Revision H Revision K Service Manual ELISYS UNO 103 106 104 106 Service Manual ELISYS UNO Bemerkung zu Service Manual 173502 Das Printfile dieses Manuals ist nicht vollst ndig Vorgehensweise Diese Seite entnehmen und durch die Kopiervorlagen ersetzen Appendix F Schematics Revision J 9 Seiten Appendix F Schematics Revision K 9 Seiten Appendix F Schematics Revision L 12 Seiten Appendix F Schematics Revision N 12 Seiten gez S Ohse 02 08 06 Human Gesellschaft f r Biochemica und Diagnostica mbH Max Planck Ring 21 e D 65205 Wiesbaden Germany Telefon 49 6122 9988 0 Telefax 49 6122 9988 100 eMail human human de Internet http www human de 01 2006 06
40. Manual ELISYS UNO 65 106 4 4 Electronics Rack 4 4 1 AC Wiring Layout See Block Diagram in Appendix C Relay Power Power PCA Supply 1 Supply 2 AC Assembly DC Junction PCA Figure 4 4 1 Electronics Rack Rear PCBs 4 4 2 Board Replacement Each of the primary circuit boards is mounted to the top of the electronics rack with three 4 Phillips head screws and internal tooth lock washers To remove a board first unplug all of the cables attached to it being careful not to bend any of the pins If removing the Daughter board gently pull the tubing from the pressure and vacuum sensors or use a small flat bladed screwdriver to push the tubing off of the sensor Then remove all three screws If removing the Coprocessor or Daughter board gently pull the board horizontally away from the bus interconnect the right angle headers at the edge of the boards See Electronics Rack figure 1 3 11 Follow the reverse procedure to install a new board First insert the three screws through washers then reconnect all of the cables A diagram of the cables to each board is provided on the inside of the top cover as well as in this manual If any of the other circuit boards should need to be replaced the procedure is similar Each board is mounted with two or three screws each with a lock washer Be sure to note where each cable plugs into the board and also note the orientation Diagrams of each board are provided in this manual 4 4 3 EPR
41. OM Replacement A firmware update will consist of one or two EPROMS to be installed in the instrument To access the circuit boards turn off the instrument and lift the top cover The EPROM labeled MAIN goes in the Main board which is the board on the left side of the instrument The EPROM labeled COP goes in the Coprocessor board which is the middle of the three boards The procedure for installing an EPROM is given below and is the same for both EPROMs 66 106 Service Manual ELISYS UNO Human e Locate the EPROM socket and lift the small lever at the end of the socket This frees the EPROM for easy removal and insertion e f necessary gently bend the pins of the new EPROM so that the pins are perpendicular to the EPROM Hold the EPROM by the body and bend the pins against a flat surface such as a table to bend all the pins the same amount Do not over bend the pins since they are easy to break if bent too far AM Figure 4 4 3 Bending EPROM e Install the EPROM with the notch oriented as shown in the label on the EPROM socket The notch should face toward the left side of the instrument Press the lever down toward the PCB until it locks into place e Turn the instrument back on with the ELISYS UNO software running on your computer and verify that the instrument starts normally 4 4 4 Fuse Replacement An inactive instrument may result from a blown fuse However a blown fuse may indicate a problem with the power
42. Service Manual ELISYS UNO Human REVISION LIST OF THE MANUAL DATE Rev REVISION DESCRIPTION 01 2006 06 First edition CONTENTS 1 INTRODUCTION nennen 1 1 1 ELISYS UNO General Description mn 1 1 2 Warnings and Precautions EE 2 1 3 Layout m mann nn nn nn 6 1 4 Technical Specifications 20 2 PRINCIPLES OF OPERATION ree ree r eurer nenne re TTL 22 2 1 System Control Electronics Rack n nn rr nnn nnn rrr T MM MMMM M M 22 2 2 Main Control Board ener nnn nnn nn nnn nnn rn nn MT MTMMM MMM M 23 2 3 Coprocessor Control Board eon n rr tnn rn nn nnnm nnn MT MTM 24 2 4 Daughter Control Board nn 25 2 5 All Junction Boards nenn nn rn nnn nnn MTM 26 2 6 Main and Coprocessor eoe n rr nnn rn rn nn nn nn nn rn nn MI MTM MMMMM MM 30 2 7 Power Supply nnn r nnn rn nn nn 31 2 8 Watchdog Circuit eorr rr nnn nnn nn rn nn nnnm nmm MTMMMMMMMMMM 32 2 9 Motion Control ee 33 2 10 Plumbing eege 34 2 11 Bottle Fill Level Sensing
43. Towards the rear of the photometer are two 6 screws which are used for providing additional support and leveling the lamp bracket These screws should be firmly touching the underside of the lamp bracket To adjust them remove the lamp bracket and loosen the hex nut at the leveling screw base and turn the screw the necessary number of turns to level or provide support for the lamp bracket Retighten the hex nut when finished 4 5 4 Exhaust Fan To control the amount of heat generated by the four xenon lamps of the photometer the lamp housing of the photometer is coupled to a fan located to the right of the machine by a 10 length of corrugated PVC tubing The tubing is held in place at the end by fitting a corrugated ridge into the fan coupling and may be released by pulling it free Should the lamp housing need to be removed release the tubing from the ELISYS UNO at the fan coupling in the rear first When replacing the lamp cover seat the cover on the lamp bracket taking care to route the red lamp power wires through the exit notch in the housing Replace the tube in the fan coupling by pressing one of the tube s ridges through the hole in the coupling until it snaps into place Do not press more than one ridge through as that may interfere with the motion of the exhaust fan The lamp housing when properly ventilated will remain slightly warm to the touch 4 6 Photometer Assembly Placement 4 6 1 Adjust Photometer Lamp Alignment To
44. aceable racks and custom designed racks are available for various bottle sizes Reaction vessel standard microwells strips or plates Instrument bottles e 2L wash with low volume warning sensor e 1L rinse or 2nd Wash with low volume warning sensor 2L waste with full warning sensor e 1L priming bottle For EIA s incubation of each row of 8 is timed separately Thermal control plate well 25 C 37 C or remains at ambient Temperature controlled to 25 C providing the ambient room temperature is below 25 C The reagent and sample racks are not temperature controlled Wash head 8 probe automatic prime and rinse Programs create and run user programmable protocols aspirate dispense soak can wash wells for re use as applicable Reading Optical design reads absorbance in 4 simultaneous channels NIST traceable calibration user selects monochromatic or bichromatic results Light source tungsten halogen lamp 8 position filter wheel 340 405 450 505 545 600 630 700 or custom Interference filters long life hard coat ion assisted deposition 2nm 10nm typical half bandpass Linear range 0 2 to 3 0A Photometric accuracy e 1 of the reading 0 005A from 0 to 1 5A e t 296 of the reading 0 005A from 1 5 to 3 0A Format One 1 compact disk Operating systems Windows 95 98 or Windows NT 4 0 Minimum system Pentium 133 MHz 32 MB RAM 20 MB free HD VGA requirements monito
45. align a lamp make sure the ELISYS UNO is powered on Move the plate carrier bed 2 to the right by giving a PLXM0200 command in the telix mode window of the control software Turn the self homing feature off by issuing a HOFF command If the lamps are off at this point turn them back on by issuing an ILPON command Insert a white piece of paper on top of the channel block underneath the aperture block of the lamp bracket You will see four white elliptically shaped spots Adjust the spot of the lamp by raising or lowering the lamp with a pair of needle nose pliers taking care not to short the leads until it is in focus and centered over the channel block hole You may need to slightly rotate the lamp as well Remove the paper and check the filter voltages by executing an RFLT command Repeat the procedure until the voltages are in the acceptable range and replace the lamp cover Refer to figure 4 5 2 4 6 2 Photometer Mount and Adjustment The photometer assembly is retained in a mount assembly by a 4 long 10 32 pin The photometer is designed to be removed by first unscrewing the retaining pin disconnecting the cables from the photometer junction board and then sliding the photometer assembly out of the mount and through the right side of the machine see figure 4 7 2 The mount assembly serves to provide a fixed reference platform for the photometer with respect to the plate carrier assembly To adjust this position loosen the two 8 machine screw
46. ameter using the command PARM4Yyyyy You have now established the center point of the index bracket search windows These parameters will be used to determine the search range before a x and y slot error is generated in the event the index slot is not detected 6 2 Rack 1 Rack2 Aligning the racks Select either Align Rack 1 or Align Rack 2 from the Instrument Setup dialog When prompted remove the rack and select OK The probe will move to the alignment point on the rack as shown above right Use the arrow buttons in the setup dialog to move the probe and racks double arrows move a large distance single arrows will move a smaller distance red arrows will move the rack in and out of the instrument blue arrows move the probe left and right green arrows move the probe up and down When you move the rack or the probe the instrument automatically raises the probe To lower it again use the button showing the probe being lowered Click Done to save the new setting or Cancel to keep the old one 6 3 Dispense Aligning the dispense position Select Align Dispense from the Instrument Setup dialog Insert a plate or strip tray into ELISYS UNO when prompted then select OK The probe will move to well H01 and lower into the well The probe tip should be roughly centered in the well and touching the bottom Use the arrow buttons in the setup dialog to move the probe position if necessary double
47. an 4 5 1 Photometer Output Check Go into Telix mode and enter the command RFLT All four channels should have 2 10 volts on all wavelengths If any are less than 2 volts consult with the manufacturer Never adjust trim pots on the Coprocessor PCB to achieve the proper voltages 4 5 2 Lamp Replacement The lamps should not be handled with bare hands Any oil from your hands will cause the lamp to burnout and fail Install the new lamp in the place of the bad lamp You will have to trim the wires of the lamp to make it fit Match the bend of the other lamps Next you will have to align the lamp The tip of the bulb should point straight down The light should project straight into the photometer Retest the voltage using the RFLT command A lamp should be replaced only if it fails to light Contact your distributor if filter voltages are reported as low Materials and equipment e Replacement lamp e Phillips screw driver e Flat blade screw driver Procedure e Get e power switch to OFF O Open the instrument refer to Figure 4 5 and open the lamp cover CAUTION Lamp is HOT Allow the lamp to cool before handling e Refer to Figure 24 5 2 a and b Lamp Replacement Loosen but do not remove the lamp terminal screws Do not loosen or remove any other screws Remove the lamp by lowering it out of the connector e Use a pair of pliers or tweezers to handle the new lamp Avoid handling with bare skin since the oil from your skin can
48. an While in motion as each filter passes in front of the photodetector an infrared optical switch triggers a sampling of the peak voltage by the Coprocessor board The four voltages are then fed to four comparators which each compare the sampled voltage to the output of an exponential capacitor decay circuit The pulse at the output of the four comparators enables individual 16 bit counters in two 8254 programmable timers The counts are read by the microprocessor and this completes the analog to digital conversion The photo detector output is proportional to the intensity of the light whereas the width of the positive phase of the comparator output is proportional to the absorbance The resistance across the log cap RC decay determines the base of the log 10 for absorbance and is used to adjust the low end absorbance calibration gain Another four potentiometers are used to adjust the high end absorbance offset of each channel WAVEFORMS MEASURED AT Jd VALE READING BLANK AIF sf d d q L A d Ei J4 1 FHOME i N EK ye ad m om r ee Eee pp en a i n mt I N 1 1 s4Y VH TEN JE 2 E x A H d it ID SON Fon I Re MINIMUM PEAK HEIGHT ZU MAX OU F mE Ge ID B n pre qu E 24 J n idi NES aft fff MN HAT ger D pd ban Vs A nat E uS U E 30 m l BASELINE HASH WAVELENGTH ANALOG PEAKS Figure 2 13 W
49. an now be lifted from the chassis Install new syringe pump by reversing steps 6 3 Remove Syringes see procedure T connector and Valve from the old pump and transfer to the new one using the 3 32 hex key supplied in the ELISYS UNO tool kit to remove the four metric socket head screws retaining the T connector and valve Service Manual ELISYS UNO 77 106 4 9 2 Syringe Replacement The 2 5 mL and 50 uL syringes are luer lock type and are retained by a nylon set screw at the base of the syringe To remove a syringe first loosen the thumb screw at the base and then rotate the syringe in a clockwise direction to loosen the syringe from its luer fitting When replacing a syringe make sure that the thumb screw is tight and that the syringe is firmly seated onto its luer fitting by rotating the syringe counter clockwise until it can no longer be turned without great effort Periodically check to make sure that the syringes are tightly held as any looseness or backlash will affect the results of the pipetting Do not remove and clean plunger of syringe Doing so will require replacement of entire syringe Figure 4 9 2 Syringe Pump Diagram 78 106 Service Manual ELISYS UNO Ss Human 4 9 3 Fittings Tubing fittings etc ELISYS UNO tubing is 18 GA standard wall TFE for the feeder and syringe interconnect lines Fittings are 1 4 28 and are made of Delrin Tubing is flared at the ends and an o ring is used with the fitting to compr
50. ature Recommended operating less than 85 humidit Certifications e NRTL listed e CE marked Design and instrument specifications are subject to change without notice Service Manual ELISYS UNO 21 106 2 Principles of Operation 2 1 System Control Electronics Rack ELISYS UNO has three large printed circuit boards which regulate and control the system All three boards are mounted to the top of the electronics rack easily accessed by lifting the top cover of the instrument On the underside of the electronics rack visible only after the back cover of the instrument has been removed are the two switching power supplies and associated junction boards See Figures 1 3 11 Electronics Rack 2 2 Main PCB Connections 2 3 Coprocessor PCB Connections 2 4 Daughter PCB Connections 2 5 8 DC Junction PCB Connections and 2 7 Back of Electronics Rack The board on the far left as you face the front of the instrument is Main 2900 100 This board contains one of the two Z180 microprocessors The other microprocessor can be found on the Coprocessor 2900 200 board which is the middle of the three boards The Coprocessor board has a direct bus interface to the board on the right known as the Daughter 2900 300 The functions and communications between these boards are described in the next sections on the following pages 22 106 Service Manual ELISYS UNO Human 2 2 Main Control Board External inter
51. aveform Service Manual ELISYS UNO 37 106 2 14 Computer Connections The PC communicates with ELISYS UNO via the serial port on the back of the instrument The connector is a standard 9 pin serial port and the cable from the instrument to the PC is a standard Null Modem Cable Null Modem Cable is a cable made with a female 9 pin connect to another female 9 pin connector In this type of cable the RX TX receive and transmit and the CTS RTS lines are crossed The serial communication is at 19 200bps 8 data bits no parity and 1 stop bit Internally the serial port is connected to the Main board 2900 100 The microprocessor on this board is responsible for communication with the PC and the Coprocessor and for routing commands within the ELISYS UNO Computer ELIS S UNO fina 38 106 Service Manual ELISYS UNO Ss Human 2 15 Computer Software Interface 2 15 1 Explanation of terms Each of these terms is explained in more detail in the Owner s Manual Patient Database Stores complete patient records including name address and doctor information Sample Protocol Procedure for aspirating and dispensing reagents or samples Wash Protocol Procedure for washing wells Method Calculation modes and parameters for an assay ASSAY A test made up of protocols parameters and options necessary to run a test Panel A group of assays t
52. be safely used as a disinfectant Take special care not to spill liquid inside the instrument Particulate matter in wash solutions can clog washer probe head easily See the section on cleaning the probe head for special instructions on removing particulate matter from clogged washer heads Please take time to read this manual carefully before using the instrument For best results familiarize yourself with the instrument and its capabilities before attempting any clinical diagnostic tests Refer any questions to your instrument dealer 2 106 Service Manual ELISYS UNO Human Retain the original packing material for future use in the event that the instrument is placed in storage shipped to another location or returned for service Two people or more should lift the instrument by placing hands under the side panels and lifting ELISYS UNO should be installed on a sturdy level surface capable of supporting the instrument s weight 45 kg safely The instrument should be surrounded by the following clearances 46cm on each side 117cm on top 15cm front and 18cm back ELISYS UNO requires no fastening to the bench top IMPORTANT OPERATING PRECAUTIONS The quality of washing often affects the validity of test results To assure adequate washing e Perform periodic dispense volume repeatability checks as described in this manual e Rinse the wash head and probe after use e Handle and store the wash head carefully to prevent dama
53. coil temperature control is on See the commands COON and COOF 3 12 Carrier Temperature Control ELISYS UNO contains an auxiliary temperature control channel labeled on the schematic as Carrier This is not presently used 3 13 External Temperature Probe ELISYS UNO also contains a facility for using an auxiliary temperature probe To use this plug the YSI style probe issued to each distributor into the jack on the front of the electronics rack then place the probe in the position to be measured Use the command PTON to turn on the external probe The Telix Mode window will display the temperature under Ext whenever a STAT is received or send XMPT0060 to see external probe temperature displayed for the next 60 seconds This probe is measured in a similar manner as the plate thermistor using the amplifier U17B and calibrated at the factory via trimpot R23 on the Daughter board Also see section External Sensor For calibration see 5 3 External Sensor 58 106 Service Manual ELISYS UNO Human 3 14 Serial Port ELISYS UNO uses a standard RS 232C serial port to communicate with the computer The provided cable is a standard null modem cable which crosses the RX TX receive and transmit and CTS RTS clear to send and ready to send lines The CTS RTS pair is used by ELISYS UNO for handshaking The DTR DSR pair is not used Communication is at 19200 8 N 1 For more information see the command list The instru
54. d go up RN EE Parameter exceeds allowed range WEE Too few parameters OOS cc ed a a KIA ERR Command has not been implemented bOA 2922 Pixs hea nin n ES Plate X axis is jammed PO OD EEN Plate Y axis is jammed psp Wash axis is jammed the motor stalled while the instrument was attempting to move the wash head Check for mechanical obstructions broken belts and opto alignments Check motor driver U11 and associated logic rp Unknown coprocessor command oU EE Invalid dispense volume 25 350 allowed ZOG ee Wash head can not be moved unless PLX gt 210 RH Can not dispense unless PLX gt 210 POW TEEN Wash movement error Wash aborted see 506 NEE Waste bottle is full empty the Waste Bottle The instrument detected a full condition on the waste bottle If waste bottle is empty dry waste cap at terminal entry points Check sensor lead is plugged in properly Check the sensor lead for shorts Wrong cap has been used Make sure the cap with the short wires is inserted into the bottle and closed snug WE Wash bottle is empty b TA E Rinse bottle is empty Wash Rinse Bottles are low The instrument detected an empty condition on the wash or rinse bottle If the bottle is full check that the sensor s lead is securely connected to the bottle cap and plugged in properly Check the sensor leads for continuity Wrong cap has been used Make sure the cap with t
55. e remove and reinstall the o ring onto the fitting as indicated Pull the probe upwards until it is free from the heating block Install the new probe into the heating block Do not fully seat it against the heating block until you insert the stainless fitting and screw it into place finger tight Proper care should be taken not to cross thread the fitting into the hole Fully seat the probe body against the heating block by making sure the black o ring at the end of the probe body is seated firmly against the heating block This assures that the probe is seated to the proper depth While firmly pulling down on the probe body retighten the set screw Do not overtighten or the probe body may become damaged Plug the green 4 pin connector into the junction board Verify that the probe is connected correctly Service Manual ELISYS UNO 63 106 e Send a WPRB to ensure the probe is filled with Deionized water Then send a PCNT The average and instantaneous counts should be approximately the same 3 and in the range of 2 3000 e Verify that the probe connection to the heat block is secure and that the probe is free of obstructions by performing a WPRB command Junction Board Heat Coil Block Connector Probe 4 2 Bottles 4 2 1 Clogged Tubing Locate clogged tube Take tubing between finger and massage debris loose Also see Tubing Replacement 4 2 2 Bottle Sensors Make sure wires are straight and not touching T
56. e all ratings and markings on the instrument Consult this manual for further ratings information before making connections to the instrument e Do Not Operate Without Covers Do not operate this instrument with covers and panels removed e Use Proper Fuse Use only the fuse type and rating specified for this instrument e Avoid Exposed Circuitry Do not touch exposed connections and components when power is present e Do Not Operate With Suspected Failures If you suspect there is damage to this instrument have it inspected by a qualified service person e Provide Proper Ventilation Refer to the installation instructions for details on installing the product so it has proper ventilation e Do Not Operate in Wet Damp Conditions e Do Not Operate In An Explosive Atmosphere e Keep Instrument Surfaces Clean and Dry PAS The operation of ELISYS UNO may involve the use of biohazardous material Refer to your owner s manual for biohazard warnings WARNING 4 106 Service Manual ELISYS UNO Human 1 2 4 Safety Terms and Symbols The following terms appear in this manual DANGER indicates an injury immediately accessible as you read the marking WARNING indicates an injury hazard not immediately accessible as you read the marking CAUTION indicates a hazard to property including the product BIOHAZARD indicates biological agents that can cause disease in humans Lab workers handling potentially infectious mate
57. ead by precisely timed openings When the control valve is open to ambient and the pump is running the pressure will not change and only vacuum will be produced by the pump The vacuum side of the pump is connected to the waste bottle Vacuum is monitored by microprocessor control by monitoring the output of solid state pressure sensor circuit P1 and U6 on the Daughter PCB and switching the pump on and off accordingly Vacuum is not regulated but is monitored Refer to Error Messages Status Indicators and Plumbing Bottle Level Sensing 2 10 3 Wash System Dispense The pump produces 34 5 kPa air pressure at the top of the wash and rinse bottles The fluid is forced up from the bottle but is stopped by a solenoid pinch valve The valve is opened for a precise interval under microprocessor control to allow fluid to flow into the dispense cavity of the wash head where it is distributed to stainless steel capillary tubes and then into eight microwells Aspirate A vacuum is applied to the waste bottle any time the pump runs The pump is connected to a fine hydrophobic aerosol filter then connected to the waste bottle The filter prevents liquid from entering the pump The waste bottle also connects to the aspirate cavity of the wash head The vacuum is distributed across stainless steel capillary tubes thereby aspirating the contents of eight microwells simultaneously from the plate Service Manual ELISYS UNO 35 106 2 10 4 Draining
58. ed for normal operation but they can be used to fix some problems relating to data integrity To use these functions type the above command into Telix window as if sending to the instrument wegl EE Show Summary of these commands SJPR KEE Purge all the data files from a selected job e Jes cuu TER Re La Tee Rebuild the Job Index file If the file JobIndex IDX in the JOBS subdirectory becomes damaged this function can be used to restore it This make take a long time to complete if the computer contains a large amount of data IRIX arrasa ne eee EE Searches for damaged job and data files on the computer and prompts to fix them SR Rebuild the Assay Index file If the file AsyIndex IDX in the ASSAYS subdirectory becomes damaged or assay files have been manually moved this function can be used to force the index file to be rebuilt I el Oe Resets all instrument parameters to last known values Instrument will then restart Use with caution end EE EE Restores registry settings to program defaults See Registry section 3 1 9 Available Tests Commands example 9612 0 5 1 37 4 E F F w Next Rd none lt CR gt Commands listed below start with a followed by the four letter command code and any additional necessary parameters The line ends with a CR carriage return example PLTM02000300 lt CR gt If the command is invalid an error message will be sent back If the command is valid the entire command wi
59. ess against the flare to make a water airtight seal Never over tighten the fittings this could damage the seat of the valve or strip the threads of the fitting or T connector and cause an air leak Fittings should be tightened by hand only Should the tubing become excessively kinked or the o ring or fitting becomes damaged it will need to be replaced 4 9 4 Repair Tubing The general procedure for replacing the tubing is as follows e Cut the length of tubing necessary for the job Feeder Line 18 GA SW TFE 3 5 feet 1 07 meters syringe interconnect 18 GA SW TFE 8 inches 20 32 cm Cut this tubing evenly with a sharp knife e Remove old tubing For the syringe interconnect tube unscrew the fittings from the valve and T connectors at both ends For the feeder line unscrew the tube fittings at both ends and cut the fittings off Leave the old cut tube in place for now Flare the tubing at the T connector end so that the new tubing can sleeve inside Sleeve the new feeder tubing inside the old tubing and pull the new tubing through the cable carrier by pulling the old tubing at the probe mover end When the new tubing passes through the cable carrier discard the old tubing leaving the new tubing in place e Install the Delrin fittings at each end first followed by the washer and then the o ring Use the o ring to hold the fitting in place a few inches away from the tubing ends e Using a flaring tool make a flare at each end of
60. f Turns the coil and plate temperature off 40 106 Service Manual ELISYS UNO Human 3 TROUBLESHOOTING In this section each subassembly or component group is discussed and possible problems and solutions are outlined 3 1 Service Tools 3 1 1 Test Mode When servicing the instrument it is often necessary to be able to send commands directly to the ELISYS UNO This can be done by selecting Telix Mode from the View menu This mode displays the communication with the instrument and allows the commands to be typed in and executed For an explanation of the ELISYS UNO commands see Service Tools Software Service Commands Caution It is possible to damage the instrument by sending incorrect commands 3 1 2 Status Indicators Main Board There are two 7 Segmented LEDs D1 shows the index pulses Probe X ProbeZ Count Count Rack 1 Rack 2 Count A Count D2 shows the homing Probe X Probe Z Home u ME a Home Rack 1 Rack 2 Home Home Service Manual ELISYS UNO 41 106 Probe Sense Board LED status indicators on the Probe Sense PCB show whether or not the heat is on LED D11 shows the heat for the heater coil LED D8 shows the probe heat Coprocessor Board LED D10 shows the status of power supply and should always be on Daughter Board LED D12 Plate Y slot indexing LED D13 Plate X slot indexing LED 7 Segment Washer Count Plate X Plate Y Count
61. face to computer RS 232 Serial Interface Interface to Syringe Pumps RS 232 Serial Interface Position Detection and control of o O O O Probe Z Probe X Rack 1 Rack 2 Probe Coil Heating Probe Level Sensing CSI O Interface to Co processor Probe Com 2 mo Probe Z Probe X Service Manual ELISYS UNO 23 106 2 3 Coprocessor Control Board gt VV V ON Photometer Filter wheel Lamps via control of power supply 2 Bus interface to daughter board Power Supply 2 Power Filter Photometer Fan CSI O to Co processor 24 106 Service Manual ELISYS UNO AN Human 2 4 Daughter Control Board Position Detection and control of e Wash head e Plate X e Plate Y gt Plate Mixer v Plate Heater Temperature Sensor External Temperature Sensor gt Pressure Vacuum control e Pump e Pressure and Vacuum Sensors Valves Rinse Wash Bleed Pressure Ctrl gt Bottle Liquid Level Sensors Plate Mover Plate Board Coprocessor Power Mover PCA T Bottle t D rm g Sensors I Pressure Sensor LED External Display Temperature Plug Service Manual ELISYS UNO 25 106 2 5 All Junction Boards This section describes these additional boards their dedicated roles and their locations Figure 82 5 1 Probe Sensor Heater Control PCB Connections The Probe Sensor and Heater Control PCB control the fluid sensing and heat contr
62. ge e Use the prime cycle before each wash e Watch the instrument to see that the probe and wash head dispense is functioning properly Be sure to run a sufficient number of controls in each assay If controls are not within their acceptable limits or if you suspect incomplete or non uniform washing disregard test results Do not operate the instrument if the pressure is unstable or if the probe or wash head probes are damaged Service Manual ELISYS UNO 3 106 1 2 2 General Safety Summary Review the following safety precautions to avoid injury and prevent damage to this instrument or any products connected to it To avoid potential hazards use this instrument only as specified N Only qualified personnel should perform service procedures Contact your dealer to arrange factory training WARNING 1 2 3 To Avoid Fire or Personal Injury e Use Proper Power Cord Use only the power cord specified for this product and certified for the country of use e Ground the Product This product is grounded through the grounding conductor of the power cord To avoid electric shock the grounding conductor must be connected to earth ground An optional method is to attach a ground strap from the external grounding terminal on the rear panel of the instrument to a suitable ground such as to a grounded pipe or some metal surface to earth ground See Figure 1 3 4 e Observe All Terminal Ratings To avoid fire or shock hazard observ
63. hat are frequently run together Job Also known as a worklist it contains the list of patients or patient IDs and assays to run Service Manual ELISYS UNO 39 106 2 15 2 Navigating the software When you first start ELISYS UNO you will be asked to log in with a username At present this is used only for record keeping as this username will be printed in reports It is not necessary to enter a password ELISYS UNO uses the standard Windows 95 98 Windows NT 4 0 controls windows and dialogs If you are unfamiliar with these controls and how to use them please refer to your Windows documentation All of the ELISYS UNO functions are available from the pull down menus at the top of the program Some of the more common functions are also available from the main menu 2 15 3 ELISYS UNO status window This window displays the current status of the instrument including the temperature and the status of the waste wash and rinse bottles Also shown are the currently loaded racks and plate The Functions button will start the Instrument Functions dialog described in the Owner s Manual The Wash Wells will wash all twelve rows of the plate currently loaded in the instrument Use the New Wells button to tell the software that you have inserted a new plate or set of wells All wells will be marked as available The software automatically keeps track of which wells in the plate have been used To manually edit this setup or to mark ce
64. he long wires is inserted into the bottle and closed snug The black plastic separator should be at the bottom of the wires and the wires should not be crossed or touching each other Ee Filter wheel error wheel is not rotating Check for NMI interrupts at the processor 510 ico e aO OE UE Y ORTA Pressure system error pressure not building rapidly enough check valves bottle caps and tubing 52 106 Service Manual ELISYS UNO Human RH Vacuum system error no vacuum detected check pump and tubing PONG dOEEN Possible Aspiration failure wash head aspiration check failed Vacuum changes 5 inches when aspirate tubes make contact with flat surface Check pump tubing and wash head b19 EE Vacuum over range vacuum level too high gt 10 inches Hg Check for obstruction in the head or tubing DA xc EE Y slot not detected plate X registration slot not detected Check sensor at X LED on daughter board 6524 E X slot not detected plate Y registration slot not detected Check sensor at Y LED on daughter board WEEN Lamp X Failure one or more of the lamps is not operating correctly MAC ME ER Channel Blanks are not valid blanks have been reset Load wells A01 DO1 DO4 and A04 with 200uL of uncolored wetted solution such as blanking solution provided and run the command BLNKAO1 3 3 Valves Make sure power is off Each valve uses a short length of si
65. he Daughter board provides 12V to its systems through a Relay LR2 which is in turn driven by the re triggerable one shot comprised of C27 R17 and U13A The microprocessor re triggers U13A at intervals smaller than the one shot period If the microprocessor system crashes or otherwise malfunctions U13A will time out and shut down the valves and pumps by turning off Relay LR2 If this occurs the middle bar of a seven segment LED on the Daughter board will be turned off to provide a visual indication on the Daughter board 32 106 Service Manual ELISYS UNO AN Human 2 9 Motion Control Refer also to section 3 5 Motor Control for general information on motion sensing Racks Two bidirectional DC motors under microprocessor control turn 3 8 16 machined stainless steel lead screws via neoprene belts and nylon pulleys One motor is dedicated to Y motion of the rack 1 reagent the other to the Y motion of the rack 2 sample Attached to the lead screw is an encoder wheel which has ten holes that create pulses when passing between an IR LED and a phototransistor creating tachometer pulses provided to microprocessor These tachometer pulses are provided to the microprocessor at the CLK1 input and CLK2 input at 8254 U5 Micro switches positioned at extremes of rack movements disable the motor drivers U22 via NAND gates U18 when the travel limits are reached Two switches additionally indicate the home positions to the microprocessor LEDs D
66. he plastic spacer located at the bottom of the wash rinse wires should remain there providing the wires with a guide 4 2 3 Hydrophobic Filter Replacement The filter that removes contaminant particles from the exhaust of the vacuum pump must be replaced if clogged or damaged f the Aspiration Failure error message appears or if the instrument takes longer to get up to pressure the filter may be clogged If the waste bottle is overturned and the filter gets wet the filter must be replaced 64 106 Service Manual ELISYS UNO Ss Human To replace the filter e Set the power switch to OFF O e Release Pressure from bottle set by loosening the lid on Wash and or Rinse bottles e Pull the tubing from the fittings on the filter e Install the new filter with the INLET side pointing toward the waste bottle Push the tubing on the fittings until seated 4 2 4 Pressure Leak Check all bottle caps for tightness fitting connections tubing condition and pressure control valve If tubing needs replacing see Tubing Replacement section If valve is failing see Valve Replacement section 4 3 Chassis 4 3 1 Dome Lamp Replacement Figure 4 3 1 Dome Light Diagram To replace the light bulb e Turn off the power e Remove Dome Lamp cover e Twist light bulb and pull out e Install new light bulb by inserting and twisting the light bulb Replacement light bulb is a 12 volt 20 amp tubular 3 1 4 and replace cover Service
67. he plate will be moved under the wash head and the wash head will lowered into the wells Use the arrow buttons to adjust the position of the plate and wash head so that the aspirate Rack 2 needles are centered left to right toward the back of the wells and touching the bottoms of the wells The double arrows will move a large amount and the single arrows a smaller amount Use the blue arrows to move the plate left and right Use the red arrows to move the plate in and out Use the green arrows to move the wash head up and down When the plate is moved the wash head will automatically be raised To lower the wash head into the plate use the button showing the head being lowered Click Done to save the new setting or Cancel to keep the old one 2 Aspirate tube must not touch back side wall of 1 Aspirate tube must be as Figure So 3 Washer Alignment Service Manual ELISYS UNO 91 106 Appendix A Selected Part Illustrations KKK 187104 Prime Bottle 92 106 Service Manual ELISYS UNO Human 994111 Reagent Rack Sq other styles available 994113 Sample Rack other styles available D gl eg Ei c N v E a vo 3 o 4 eo be od v on x LH p uv 3 wn PS 82 o e 93 106 Service Manual ELISYS UNO 105161 Pump Ba ra 990113 Plate Cover 997420 8 Way Wash Head 990101 Aerosol Shield
68. igure 3 5 1 Typical Optical Switches With and Without Resister 54 106 Service Manual ELISYS UNO AN Human 3 6 Incomplete Aspiration One or more aspirate tubes may be clogged Clean the aspirate tubes using the cleaning wire Check the tubing path leading from the wash head to the waste bottle for kinked or pinched tubing Verify the vacuum using a gauge Check the exhaust filter for clogging Aspirate tubes should contact the bottom of all 8 wells simultaneously while aspirating not necessarily while positioning 3 7 Incomplete or Inaccurate Dispense One or more dispense tubes maybe clogged Clean the dispense tubes using the cleaning wire Check the tubing path leading from the wash or rinse bottle to the wash head for kinked or pinched tubing Check for stuck valves and clogged or pinched valve tubing Check the bleed valve for leakage Verify the pressure using a gauge If the instrument has been stored for an extended period without rinsing it may be necessary to unclog the tubing and or operate the valves manually in order to prime the instrument 3 8 Photometer The photometer consists of a mechanism with electronics and additional calibration and analog to digital conversion circuitry on the Coprocessor PCB It can be divided into several component systems e Lamp lamp terminal blocks related mounting hardware brackets and adjusting screws e Photometer PCBs for light detection speed and position sensing e Filte
69. indicator LED will show see Troubleshooting Status Indicators the movement of the axis On the sensor check to see if there is 1 2 volts voltage drop at the IR LED and the IR photo transistor switches from its low state less than 5 volts to high state 24 0 volts when the eye is blocked Another possibility is failure of the L293B motor driver Each motor has its own motor driver chip See Principles of Operation Motor Control for specific locations Other problems could be with other sensors that do not have indicators motor driver electronics wiring the motors themselves or the belt The belt should be attached correctly and moving smoothly around the pulleys Probe X The Probe X axis has a two phase Encoder Wheel and matching sensor pair on the left side which provides tachometer pulses to the microprocessor at the NMI input and PB4 input at 8255 U9 CAUTION The encoder wheel is fragile and can cut you do not damage The two phases of the encoder output are used by the software to determine direction and accurately track the probe s position On the right side of X travel there is a sensor with a phototransistor and LED that is at a known fixed point and is used as a reference to verify the encoder wheel count is correct and if not resets the count in software An additional sensor to the far left of the mechanism senses home for the microprocessor LED Transistor Wires LED Transistor Wires Nor Resistor F
70. inoperative Note Tubing must be installed in the valve for it to operate correctly check inputs and outputs at U3G If the pressure vacuum pump is inoperative check Q2A If the pump and all of the valves are inoperative no voltage present at any time check LR2 and U12H for proper operation Both should be active at all times while the instrument is powered The seven segment LED D1 on Daughter PCB should have the center line illuminated See Status Indicator section 3 5 Motor Control All of the axes of motion are of the same basic design a DC servo motor and a position sensor Probe X has a two phase encoder wheel while the other axis sensors are simpler either a circular multislot interrupter disk linear multislot interruptor or plain single interrupter such as end limit sensors Openings in linear multislot interruptors are also referred to as index slots and the Service Manual ELISYS UNO 53 106 interruptors are also called index brackets The most common failure in movement can be attributed to a physical obstruction Check for this possibility first Another less likely possibility is a problem with a sensor This can usually be determined by observing the sensor s status indicator If a problem is indicated check the IR phototransistor and LED of the sensors The phototransistor and LED should be perfectly aligned so that the eyes can see each other Check the home sensor pulley count and limit sensor output The
71. ittings until seated Be especially careful not to kink stretch or tension the tubing The replacement tubing should be routed in exactly the same place as the original 4 11 Washer Troubleshooting the washer involves the following steps Drive Screw E x Plate M Mount Motor Figure 4 11 Washer Diagram Service Manual ELISYS UNO 83 106 Clogged Head See Incomplete Aspirate or Dispense Clogged Tubing Take tubing between fingers and massage material loose If needed gently exercise tubing till clog is released If you are unable to release material see tubing replacement Noisy Drive Screw If you have an aluminum drive screw rod it will squeak loudly if it needs lubrication Apply a small amount of lubrication gel included The neoprene drive belt which couples the probe motor to the probe lead screw pulley can become worn or loosen with age Remove the four flat head screws at each corner of the plate mount Lift the plate mounting upward and forward to clear the probe arm Look at the bottom of the mechanism assembly Remove the old belt by carefully lifting one side off the probe motor pulley Remove the belt from the other pulley Do not bend the optical sensors mounted on the edge of the mechanism PCB Install the new belt first to the probe lead screw pulley then to the probe motor pulley Do not bend the optical sensors mounted on the mechanism PCB Do not twist the be
72. l determine if the command must be executed by the Coprocessor This is the case for example when sending a command such as WPRI Wash Prime Since both the wash head and the pressure system are controlled by the Coprocessor it is responsible for executing this command Using the CSI O connection Main will forward this command to the Coprocessor and wait for it to be completed When the command is complete the message will be sent from the Coprocessor to the Main board via the CSI O connection and then echoed by Main back to the PC using the serial port In addition to the commands originating from the PC Main and Coprocessor communicate to coordinate a variety of functions This communication is transparent and the ELISYS UNO can be treated as a unified instrument The only time when it may become necessary for the service technician to examine the CSI O connection is if the processors are unable to communicate at startup How to diagnose and solve this problem is covered in the troubleshooting section The Coprocessor board and the Daughter board should be considered one unit The Z180 microprocessor on the Coprocessor board directly controls the peripherals on the Daughter board by means of the bus interconnect The 26 pin right angle connectors directly tie together the data and address buses of the two boards 2 6 1 Firmware versus Software The internal software usually referred to as the firmware is responsible for the direct control of
73. late carrier with regard to the photometer Mixer Shaker Mixing in wells is accomplished by shaking the Plate Carrier Y axis platform The platform is supported by 4 flexible natural latex rubber mountings and is attached to the underside of the platform a motor driven counterweight Spinning the counterweight causes the platform to shake on its mountings with proportional intensity Microprocessor control of the shaker motor via U7 on the coprocessor PCB allows for adjusting the single mix shake intensity setting Adjust the mixing using Telix command IMSPDxxxx Adequate mixing should shake 250uL of reagent and sample vigorously but without splashing up Speeds 0030 to 0050 should cover the range Service Manual ELISYS UNO 33 106 Probe X A bidirectional DC motor under microprocessor control pulls a slide via a nylon belt and nylon pulleys The motor is dedicated to X motion of the probe Probe X has a two phased encoder wheel which creates pulses when passing between a reader providing tachometer pulses to the microprocessor at the NMI input and PB4 input at 8255 U9 The one phase of the index pulley is used by the software to determine direction and accurately track the probe s position The probe X junction board contains some jumpers that should not be adjusted Another phototransistor positioned toward the right side of the probe X movement is used to verify and if necessary reset the probe count additionally Another phototransi
74. lates to reference the plate position with respect to the photometer If the instrument is out of alignment you will need to adjust the index plates Caution Alignment of the plate carrier to the four separate channels of the photometer is critical for accurate absorbance measurements to occur and must before done after aligning the photometer Reblank after aligning the photometer by using the BLNKA01 command To adjust the alignment select under the script window the script Align 1st This script consists of a large series of the same command RWELAO1721 While this script is running observe the centering of the 4 photometer apertures in the plate carrier by viewing from above The carrier should position such that the apertures are in the centers of the 4 selected well positions If this positioning is not centered adjust in turn each axis of movement by repositioning the index brackets The x index is located at the right side of the plate mover mechanism The y is attached to the plate carrier Once centering is achieved type the command YREF This will re establish the location of the y index plate 88 106 Service Manual ELISYS UNO Human Next type RWELA01721 followed immediately by POST This will display the coordinates in the status window of well A01 Write these down Next type PARM4Xxxxx where xxxx is the plx parameter taken from the status window Next enter ply par
75. licone tubing Tubing may self adhere or become clogged with dried residue from fluids If the valve operates but no fluid is dispensed or aspirated the valve tubing may be blocked To check for blocked tubing do a visual inspection Grasp the tubing on either side of the valve body and gently stretch it If needed pull the tubing from the valve body Roll the tubing between your fingers and gently stretch it Reposition and test If still blocked see the section Valve Tubing Replacement See Appendix E Fluid System Schematic 3 4 Vacuum and Pressure Systems The pump requires no maintenance If the pump runs frequently or continuously while the instrument is not in use there is probably a leak Check the bottle caps and fittings Check that the tubing is firmly seated on barbs and all fittings are tight Turn the fittings only until finger tight Do not overtighten the plastic Luer fittings Check the operation of the valves to ensure they are closing completely If the pressure pump runs frequently or continuously check the bleed valve wash and rinse bottles and pressure system tubing for leakage The bleed valve must be completely closed while the pressure system is on If aspiration is poor or absent the exhaust filter may be clogged and should be replaced In the event that the exhaust filter gets wet due to a waste bottle spill it must be replaced for continued operation See the section Exhaust Filter Replacement If any valve is
76. ll be echoed and the instrument will begin processing When the command is complete the entire command will once again be sent back but with the first character formerly a changed to a 7 example PLTM02000300 lt CR gt A line beginning with a denotes a position report It will consist of the location of each of the 7 axes in the following order Rack 1 Rack 2 Plate X Plate Y Wash Z Probe X Probe Z example 20200 0100 0225 0400 0001 1520 0050 lt CR gt A line starting with a is a status report The Telix Mode window of the ELISYS UNO software will display this information in a self explanatory categories 46 106 Service Manual ELISYS UNO AN Human Lines beginning with a are error messages The three numbers following the asterisk will be the error code followed by a colon and then the text message Error numbers greater than 499 originate from the Coprocessor example 001 Invalid number of parameters lt CR gt Lines that begin with a are additional information Returned parameters settings or configuration etc example SERN 0000 lt CR gt Coordinates are always given in 100ths of inches 0100 1 inch The home position is 0000 Note Commands from the instrument will have a checksum inserted between the end of the string and the carriage return The Telix Mode window of the ELISYS UNO software will normally hide the checksums but they will be visible if using a differen
77. lt Turn the probe motor shaft several revolutions to ensure that the belt is seated without twisting Install the mechanism assembly and replace the four nuts 84 106 Service Manual ELISYS UNO Human 5 Calibration CAUTION Any instability of calibration could indicate an instrument problem Do not calibrate anything without considering all other alternatives 5 1 Setup See Alignment section of this manual 5 2 Photometer If you feel that the photometer needs calibrating call the manufacturer 5 3 External Sensor There is an external sensor External Temp Probe located on the electronics rack to the front right side Plug in the 37 C Reference comes in distributor pack In Telix mode send a STAT command then see if Ext in the status window reports 37 C 0 2 C If not adjust the external temp pot Daughter Board R23 and repeat See caution at the top of the section 5 4 Plate Carrier Dispense 200ml water in all wells Place a plate of strips in the metal plate insert in the plate carrier and plug thermistor probe comes with distributor pack in the External Probe Sensor jack Place thermistor probe in well at the center of the plate in center of fluid in the well tape down Close all lids and covers and let equilibrate Type ISTAT Compare Ext with Pit plate Measurements should be within 0 2 C If not adjust plate temp pot Daughter R28 and repeat See caution at the top of the section
78. mechanisms See troubleshooting for a full outline of what each light means The board is located behind the Probe Shield Coprocessor Daughter Diluter One or the Diluter other s used Supply Unused Main Interior Lamps Figure 2 4 8 DC Junction PCB Connections The DC Junction board is the distribution center for DC power for the instrument It is located under the electronics rack 28 106 Service Manual ELISYS UNO Human Plate X Carrier Plate Heat Not Used Plate Carrier Optional Heat Control Figure 82 5 9 Plate Mover PCB Connections The Plate Mover coordinates the Plate X axis Plate Y axis movements the Plate Carrier incubation mixing fan and wash movements It is located to the right and under the Plate Cover Note Plate Carrier connection is currently not used The extra two position connector with two orange wires should not be plugged into the board Power Mains Coprocessor Figure 32 5 10 Relay PCB Connections The Relay board turns the second power supply off It is mounted inside the electronics rack Service Manual ELISYS UNO 29 106 2 6 Main and Coprocessor The cable from J8 of the Main board 2900 100 to J12 of the Coprocessor board 2900 200 interconnects the two processors and allows them to communicate This connection uses a version of the Z180 s CSI O Clocked Serial 1 0 When a command is received from the PC by Main the firmware on the Main board wil
79. ment s serial port must be connected to the ELISYS UNO computer software for normal operation If the instrument emits a triple beep at startup this indicates that the instrument is unable to connect to the PC If the communication times out the instrument will initialize with no connection It then must be reset using the reset button on the back of the instrument before it will resume communication with a computer Use only a standard Null Modem cable to connect to the instrument to your PC Computer ELISYS UNO nia DES ZS Lee C nos a Te Ok he pu Dee d QU EE d e Rx Dr 3 y pU d i id E a DM 5 5 L F D a JS V T Fr G RATS 17 ka EN no T s m D CTS ja i i Service Manual ELISYS UNO 59 106 3 15 Printers 3 15 1 Black amp White A black and white printer should be set to print in black and white If your ELISYS UNO computer is hooked up to multiple printers or if you print to multiple printers on a network you may encounter a problem if one of them is black and white with a color capable driver If you print on a color printer and then print on a black and white printer before restarting ELISYS UNO it might attempt to print color on the black and white printer If you encounter this problem restart the ELISYS UNO software Known printers with this problem Citizen GSX 190 3 15 2 Color Some color capable printers do not correctly print graphs on computers with
80. n counts that the software will move the probe into the liquid when aspirating The default is 6 PushVolumer sum This is the default Push Volume used when creating a new assay See the sections on assay programming in the owner s manual for more information The default is 25uL HReagentt utoft eerren Sample protocols dealing with volumes less than or equal to this cutoff are treated as samples If the volume is greater or there is a dispense to ALL the protocol is treated as a reagent This distinction controls which aspirate dispense method is used See Service Manual ELISYS UNO 45 106 the sections on assay programming in the owner s manual for more information The default is 20uL ReagentVolFactor sseeeeseeeeeereeerereeeren This is the amount of extra volume aspirated when picking up reagent This extra volume is necessary to prevent reagent dilution The default is 1 20 20 SampleVolFactor This setting is no longer used SetAirSpeed cecececececeeeeeeeeeeeeeeeeeneeeees This is the speed at which air gaps are created The default is 2 and should not be changed Parameters This is the unformatted list of parameters direct from the instrument Please see the command list an alignment sections for information on these parameters 3 1 8 Software Service Commands There are several software maintenance commands that are also available None of these functions are requir
81. nd nylon pulley The motor is dedicated to vertical Z motion of the wash head The pulley has several index holes which create pulses when passing between an IR LED and a phototransistor providing tachometer pulses to the microprocessor at the CLKO input at 8254 U16 Phototransistors disables the motor driver U11 via NAND gate U10 when the travel limits are reached Travel limits are indicated by encoder flag slot attached to the washer shuttle The 7 segment LED on the daughter board provides visual feedback to the service technician of the tach pulses and home positions The plate mover junction PCB contains the motor drivers and connects to the daughter board via a 26 pin ribbon cable The daughter PCB holds the index pulse buffer and limit logic 2 10 Plumbing 2 10 1 Pressure and Vacuum Solid state sensors P1 and P2 on the Daughter board are amplified at U6 and U8 and time division multiplexed via U9 into V F converter U14 then measured at 8254 timer U2 This provides feedback to the microprocessor for coordinating pump activity and displaying measured pressure and vacuum levels The vacuum pump is driven by U3 and MOSFET Q2A The valves are driven by U3 and U15 34 106 Service Manual ELISYS UNO AN Human 2 10 2 Pumps and Valves Refer to Figure 1 3 12 for pictorial and Appendix E for Fluid System Schematic diagram A diaphragm pump and valves under microprocessor control via the daughter PCB are switched on and off to generate
82. ng a data file with a different version than the data file was created under 3 1 4 Updating the default reports The ELISYS UNO software includes a set of default report templates including a template for each mode and for various special reports The user can edit these default reports but the software contains a way to restore them to their original form at any time This may become necessary if the user edited report templates contain errors which prevent them from being used From the Setup menu of the software select Preferences and then Default Reports The Default Reports Setup dialog will be shown Select the button Restore Update all default report templates to restore all of the standard templates It is recommended that custom reports be created by first copying one of the default reports to a new name For more information on report templates please see the Report Template Specification in the Owner s Manual 3 1 5 System Info Report The system info report provides information on the computer that is running the ELISYS UNO software To access this report select System Info from the Help menu of ELISYS UNO The Windows version section will report information regarding the Windows installation In Windows 95 the SP section will report the system revision B which do affect ELISYS UNO If the user has one of the earlier versions of Windows CAUTION There are several known problems with the versions of
83. ol for the coil block and probe This board mounts to the top of the coil block Power Sense Encoder Wheel Figure 2 5 2 Probe X PCB Connections The probe X board controls the sideways movement of the probe This board is mounted on the left side of the probe X bracket under the electronics rack Control EN Main B em Motor Power Main Probe Sense Figure 82 5 3 Probe Z PCB Connections 26 106 Service Manual ELISYS UNO Human The Probe Z board controls the up down motion of the probe This board interfaces with the Probe Sense and Heater Control board located inside the probe housing Rack 2 o Rack 1 Control Main Figure 2 5 4 Rack Y PCB Connections The Rack Y Mover coordinates the motion of Rack 1 reagent rack and Rack 2 sample rack It is located on the rear of the Rack Mover Assembly Coprocessor Photometer Figure 2 5 5 Photometer Junction PCB Connections The Photometer Junction board is the junction point for the Photometer mechanism The board is located on the Photometer Module Photometer Junction Photometer LED Figure 2 5 6 Photometer PCB Connections Service Manual ELISYS UNO 27 106 The Photometer PCB optically reads the light emitted by the lamp after passing through the plate It is located inside the Photometer housing Daughter Figure 82 5 7 LED Display PCB Connections The LED Display shows the status of various states of the
84. oltage is lower than 6 0 VDC power supply 2 or the wiring from it to the lamps may be a problem Otherwise refer to the section Lamp Replacement paying particular attention to the lamp alignment instructions Never adjust or manipulate the trim pots on the coprocessor PCB to achieve the proper filter voltages Doing so will destroy the calibration The four photometer channels the filter wheel count CNT and NMI and filter wheel home FHOME all originate in the photometer mechanism The photometer PCB contains four photodiodes and very sensitive high gain op amp circuits Two phototransistors are positioned to see through the home and indexing holes in the filter wheel The exciter for these transistors are 2 LEDs located on another small circuit board on the opposite side of the wheel outside the cover box Because the Service Manual ELISYS UNO 55 106 photodetectors and related circuitry is solid state it should require no service Do not attempt to modify the photometer PCB Channel 1 read site is located under the lamp that is toward the front right Viewed from above channels 2 4 are located counter clockwise from 1 The filter wheel turns on a shoulder screw shaft and is driven by the filter wheel motor via two neo prene belts The filter wheel itself requires no adjustments The optical interference filters contained in the wheel are of metal ion assisted deposition IAD type construction and are intended to be maintenance free f
85. on board indicates that the heat strip is on The thermistor circuit in the plate is brought through the plate junction board and back to the Daughter board where it is fed into the amplifier at U20A The circuit is calibrated using the trimpot R28 on the Daughter board The output from this amplifier is fed into the demultiplexor CD4051 U9 then into the voltage to frequency converter AD654 U14 The output from the VF is read by the 8254 U2 on pin 9 The coprocessor firmware controls both the demux and VF to read all temperature pressure vacuum and bottle sensors which use the VF circuit To check the current coil temperature send a STAT command The Telix Mode window will display the plate temperature in the top left status box under PIt To turn the plate temperature control on use the command TCON and to turn it off send the command TOFF PLATxxxx example PLAT037 for 37 degrees will adjust the target temperature Software control is enabled for 25 37 C and off room temperature Service Manual ELISYS UNO 57 106 3 10 Coil Temperature Control The tubing from the probe is wound in the coil block allowing reagents to be prewarmed before being dispensed to the plate The coil block is heated under the control of the Main board firmware to the specified temperature A change in resistance of the coil thermistor at J55 of the probe sense board causes the frequency of the oscillator U29B to change This frequency is fed
86. onnectors J50 J51 and J52 provide 12VDC 5VDC and two grounds to each of the three main boards The 5V supply should be between 4 9V and 5 25V and the 12V supply between 11 5V and 12 25V depending on the current load The second power supply is turned on and off via the relay junction board which controls the AC input power using a 12VDC relay The relay is controlled by a ULN2003A U9C driver on the Coprocessor board This second supply provides the lamp and photometer operating voltage The lamps are wired directly to this supply to minimize voltage variation Lamp supply voltage is approximately 6VDC This supply also produces 14 15VDC which is routed to the Coprocessor board at J6 Components VR2 VR3 C37 and C39 regulate these voltages to 12VDC which are used to operate the photometer circuits See Principles of Operation of the Photometer for more information 2 8 Watchdog Circuit All valves motors and pumps are powered through the major boards The Main board provides 12 volts through an IRF9530A MOSFET Q1A which is in turn driven by the re triggerable one shot comprised of C14 R5 and U12A The microprocessor re triggers U16A at intervals smaller than the one shot period If the microprocessor system crashes or otherwise malfunctions U12A will time out and shut down the motors rack and probe and coil heating power by turning off Q1A If this occurs LED D3 will be turned off to provide a visual indication T
87. or the life of the instrument However in an environment where extreme humidity or temperature are encountered optical characteristics on one or more filters may be compromised Run Photometer Output Check as described in 4 5 1 If only one or two filter voltages are low on all channels the filter s may be suspect Refer to the service procedure Filter Replacement Never adjust trim pots on the Coprocessor PCB to achieve the proper voltages The filter wheel speed is regulated by seeing CNT NMI rate and adjusting the duty cycle of the output to the filter wheel motor When the wheel rotates the waveforms shown in the Photometer Test Points diagram section 3 8 1 should be generated The electronics consist of the 4 photodetectors and associated high gain amplifiers that convert the light signals to electric analog signals and buffer followers on the photometer PCB On the coprocessor board are gain of 2 amplifiers sampling analog switches and hold capacitors followed with high impedance buffers These buffers feed voltage comparators that are comparing the held light signal voltage with a logarithmic discharging voltage ramp The output of the comparators gate the corresponding 16 bit counters clocked at approximately 2 MHz to make the analog to digital conversion The NMI clamp diodes keep the gates from enabling the counters until the wheel rotates out of a filer count hole in the filter wheel This begins the hold phase of the analog proces
88. r serial port Windows 95 98 or Windows NT 4 0 Recommended system Pentium 333 MHz 32 MB RAM 20 MB free HD SVGA graphics and monitor serial port CD drive Windows 95 98 Windows NT 4 0 Main menu options Patients New Job Job Setup 20 106 Service Manual ELISYS UNO Human Technical Specifications for ELISYS UNO Secondary menu options create edit protocols import export assays data etc Control Run Setup Calculation modes absorbance single standard factor fixed time kinetics kinetics by standard or factor multi calibrator point to point linear regressions log logit cutoff by absorbance or standard and more Self monitoring modes lamp bottle volumes filters pressure vacuum mechanical function and more QC options store control data print Levey Jennings or QC range plots calculate SDs Serial port RS232 output only 9600 Baud 1 start bit 8 data 1 stop no arity no handshake serial cable provided Voltage range 100 250VAC Frequency range 50 60Hz Power maximum 160W Installation catego CAT II Environmental Conditions for Safe Operation e Indoor use e Altitude up to 2000 m e Temperature 5 C to 40 C e Humidity 80 for temperatures up to 31 C decreasing linearly to 50 humidity at 40 C e Mains supply voltage fluctuations not to exceed 10 of the nominal voltage Recommended Environmental Conditions Recommended operating 18 35 C temper
89. rams ELISYS UNO gt ELISYS UNO Pack There is a checkbox option Unpack files and two directory file name entry boxes ELISYS UNO Export Directory and Package File Name Make sure the Unpack files option is not checked ELISYS UNO Export Directory always defaults to a ELISYS UNO If you exported your files to a different directory enter the name of that directory here Package File Name always defaults to a ELISYS UNO CWPack pck If you would like the pack file to go into a different directory or have a different name enter that information here Press the button labeled Start CWPack will now process the ELISYS UNO export files into a pack file When it is done the status window will report Pack Successfull You may now transfer and or email the PCK file to it destination Unpacking files To unpack a packed file perform the following steps Start CWPack by selecting on the Windows Start Menu gt Programs gt ELISYS UNO gt ELISYS UNO Pack There is a checkbox option Unpack files and two directory file name entry boxes ELISYS UNO Export Directory and Package File Name Make sure the Unpack files option is checked ELISYS UNO Export Directory always defaults to a ELISYS UNO Enter the name of a new or empty directory here Do not enter the name of your ELISYS UNO data directory here Package File Name always defaults to a ELISYS UNO CWPack pck
90. rials must use universal precautions to reduce the risk of exposure to these agents These symbols may appear on the product Sb WARNING Protective Ground CAUTION BIOHAZARD Risk of Shock Earth Terminal Refer To Manual Risk of Infection FUSE For continued protection against risk of fire replace only Ek with fuse of the specified type and current ratings Disconnect equipment from supply before replacing fuse DANGER Pinch points sharp points and moving parts mechanisms may operate without warning Service Manual ELISYS UNO 5 106 1 3 Layout Refer to the figures on the following pages for illustrations of the ELISYS UNO and its components WARNING servicing the instrument N Hazardous line voltages are present behind the AC cover and on the power supplies Always disconnect the external AC power cable before WARNING N The operation of ELISYS UNO may involve the use of biohazardous material Refer to the Owner s Manual for biohazard warnings ELISYS UNO Component ELISYS UNO Exterior View A NOTE For clarity of illustration the aerosol shield is not shown ELISYS UNO Exterior View B Right Side Panel Rear Panel layout Sub assemblies Plate X Mover Plate Y Mover NOTE Plate X and Y Movers are combined and are responsible for the plate carrier movement Rack Y Mover NOTE Responsible for the sample and reagent rack s movement Probe X Mover Probe Z Mover NOTE Probe
91. robe tips are sharp and may cause bodily injury Do not place hands or fingers under the probe or wash head probes while instrument is in operation Always set the power switch to OFF 0 before working on the probe or wash head Never touch the probe or wash head while the instrument is operating N The probe performs a self clean periodically while the probe is idle Keep hands away from the probe at all times when WARNING the instrument is ON 1 e If the waste bottle is overturned during operation immediately set the power switch to OFF 0 If the hydrophobic filter becomes wet due to an overturned waste bottle it will be blocked Continued use of a blocked filter will impair washer effectiveness and or result in damage to the instrument e The wash and rinse bottles are pressurized during normal operation Do not remove bottle caps or tubing connections while the bottles are pressurized Turn off the instrument before changing bottles adding more solution or tubing connections e Solvents such as acetone or thinner will damage the instrument Do not use solvents to clean the unit Avoid abrasive cleaners the aerosol shield is liquid resistant but is easily scratched The exterior of the instrument may be cleaned with a soft cloth using plain water If needed a mild all purpose or non abrasive cleaner may be used A 1096 solution of chlorine bleach chlorine bleach 5 25 Sodium Hypochlorite or 70 isopropyl alcohol may
92. rs filter wheel drive belts pulleys and jackshaft and filter wheel motor e Electronics photodetector high gain amplifiers buffers gain of 2 amplifiers sample and hold log ramp generator voltage comparators counters NMI clamp The lamps are custom tungsten filament xenon gas filled lens end bulbs rated for 6V operation and consume 6W each They are intended to be maintenance free for many years since they are turned off automatically during periods of instrument inactivity However under heavy usage the lamps remains on for longer periods and may require replacement as the output becomes low near the end of its service life This is also apparent by the blackening of the glass envelope A physical shock to the instrument may necessitate lamp replacement due to filament breakage The lamp terminal blocks and lamp guide hold the lamp in alignment with the rest of the assembly The brackets provide adjustments in horizontal dimensions so that the filament can be centered on the light path The lamp must be replaced if output becomes low or the lamp fails completely To check for low lamp refer to the service procedures section Photometer Output Check Section 4 5 1 If all or most of the voltages are low for a particular channel and not for other channels or for all channels the lamp alignment is the most likely cause If all voltages are low rule out the power supply by measuring the voltage at the lamp terminals If the lamp v
93. rt the 3 6 32 screws and snug up Do not overtighten the photometer cover screws Doing so will bend the photometer cover and create light leakage Step 7 If the lamps were disturbed during the above operations check that the lamps hang vertically down and are aimed at the screen and aperture holes If necessary refer to the lamp alignment procedure in the service manual Step 8 Replace the side cover Step 9 Attach the power cord and connect the unit to the mains supply Turn the unit on Send command IRFLT The displayed numbers represent the voltages of the installed filters All voltages should be between 2 and 10 volts Service Manual ELISYS UNO 75 106 Step 10 Fill plate wells A1 A4 D1 D4 with water treated with surfactant to create a curved meniscus on the water surface Install this plate and send command BLNKAO1 to establish new blank levels 4 8 Plate Carrier The ELISYS UNO plate carrier is designed to be able to be removed for service from the plate mover assembly In the rare event that it becomes necessary to remove the plate carrier use a 1 16 hex key to remove the set screw retaining the 1 4 stainless steel guide rod Pull the guide rod out through the front of the instrument Cut away the tie wraps anchoring the orange and violet cable of the plate carrier and unplug the cable from the plate mover junction board The plate carrier is now free to be removed Replace the plate carrier by reversing the above
94. rtain wells as not available click the Edit Plate button Note The Wash Wells and New Wells functions are also available from the Run menu 2 16 Instrument Functions This dialog gives you easy access to some of the more common instrument functions To open it go to the View menu and select ELISYS UNO functions The following options are available Note The Start of Day and End of Day functions are also available from the Run menu Start of day Prepares the instrument to be run by turning on the photometer lamps washing the probe and priming the wash system End of day Prepares the instrument to be shut down at the end of the day by turning off the photometer lamps washing the probe and rinsing the wash system Reset Homes all of the mechanisms and reinitializes the syringes Standby Parks the probe and turns off the pressure system Park probe Moves the probe to the center wash station Wash probe Washes the probe Prime wash Primes the wash system with the solution in the wash bottle Prime Syringes Primes the syringes with fluid from the prime bottle Prime rinse Primes the wash system with the solution in the rinse bottle Wash Wells Washes all twelve rows of the plate Heat to 37 C Raises the coil and plate temperature to 37 C Temp Of
95. s at the rear of the photometer and position the photometer as necessary Avoid making large adjustments The photometer mount assembly should only be adjusted if precise alignment of the plate carrier with respect to the photometer cannot be achieved by displacing the carrier along the X and Y axis For alignment with Plate Carrier travel see Alignment Photometer and Alignment Plate Carrier to complete photometer placement Service Manual ELISYS UNO 71 106 4 7 Filter Wheel 4 7 1 Filter Label The filter label located on the lamp bracket describes the specific filter wheel configuration for your particular instrument Refer to Figure 4 7 1b Filter wheel position is the physical placement of the filter on the wheel The filter wheel position numbers are shown in Figure 4 7 1a Note the position of the Index hole and the Home hole Filter Position Rotation Index Hole Home Hole Figure 4 7 1a Filter Wheel Postions Filter Wheel Part 996040 Position Filter Position Filter 340 505 Filter Wheel 700 545 No 405 600 450 630 Figure 4 7 1b Filter Label 72 106 Service Manual ELISYS UNO Human 4 7 2 Photometer Filter Replacement Materials tools required e Replacement filter set e 1 Phillips screw driver e 1 Flat blade screw driver e 1 sharp pick or pointed object Note orientation then remove the too two comectors end urolug the ground wire from the Chassis Basa 4 ZI oi
96. sing synchronizing the start of the timer counting At the same time the processor uses the NMI to control the motor speed and update the filter count in software The home is used to reset the filter count in software The calibration is affected by trimpot adjustment on each channel at the input of the individual gain of two amplifiers offset and by the trimpot TP1 on the logarithmic discharging voltage ramp generator gain Never adjust trim pots on the Coprocessor PCB Contact the manufacturer if you question the calibration 56 106 Service Manual ELISYS UNO AN Human 3 8 1 Photometer Test Points WAVEFORMS MEASURED AT J4 WHILE READING BLANK AIR 1 I Le ONE REY OF FILTER WHEEL 240 ms DURING READINGS gt I r i o TI 1 A d e m En ry u EE Llc 1 1 I D EEA E E I T MINIMUM PEAK HEIGHT 24 MAM 104 I LI 4 1 LU hl PA E IB E BPE iva wo o NUN S sd E ee EE E ELLE AN des Lec dA 4 Sample el la Hold T lt 30 mY BASELI NE HASH N WAYELENGTH ANALOG PEAKS AT di oO H I I H Lb Lb l l l U H I l ER I Home 3 9 Plate Temperature Control The plate carrier contains a thermistor and a heating element totaling 12 ohms which are used to provide the temperature control The heat element is switched on and off under software control using the MOSFET Q11 on the plate junction board LED D14 on the plate juncti
97. stor indicates the home position to the microprocessor LED D1 on the Main board provides visual feedback to the service technician of the tach pulses and home positions The Probe X Junction PCB holds the motor driver The main PCB holds the limit logic and connects to the Probe X Junction PCB via a 10 pin ribbon cable and power Probe Z A bidirectional DC motor under microprocessor control turns a 1 4 20 machined brass lead screw via neoprene belt and nylon pulley The motor is dedicated to the vertical Z motion of the probe Probe Z uses a shuttle with an attached aluminum code wheel that has index holes which create pulses when passing between an IR LED and a phototransistor providing tachometer pulses to the microprocessor at the CLKO input at 8254 U5 There is a phototransistor positioned at the extreme of the probe Z movements It will disable the motor drivers U27 via NAND gate U18 when the travel limits are reached Additionally other phototransistors indicates the home positions to the microprocessor LED D2 provide visual feedback to the service technician of the tach pulses and home positions The Probe Z Junction PCB holds the motor driver and interfaces to Probe Sense board The main PCB holds the index pulse buffers limit logic and connects to the Probe Z Junction PCB via a 10 pin ribbon cable Washer One bidirectional DC motor under microprocessor control turns a 3 8 16 machined stainless steel lead screw via neoprene belt a
98. t communications program 3 1 10 General Commands um Repeat the previous command UNITS aan Han nen Initialize home all axes IHOME ss aaa aa dd Initialize home all axes ISE RIN su zoe co E o aan Show the serial number returns SERN xxxx IPOSL tat itti e e KN E Tanki Display position of all axes ISTAT 1444443448488 iu ru ets Display temperatures vacuum pressure status of bottles INE V Sneton hee Report the software revisions IPARM iudi e ee Display the current parameters IPARMnappDppp i ite Edit a parameter n par a par label xyz ect pppp new par value US e Move plate X axis to home IPISY E iiie iiie tied de ede e ete eiie Move plate Y axis to home iz EORRRRSPERERERERFIEREEFFEFRRERFEESERFEETEFERFERFETERERERE Move both plate X amp Y to home IPIEXIMDOOOC en Run Move plate X axis to coordinate xxxx IPLYMyyyy ue Move plate Y axis to coordinate yyyy IPETIMDOOOQ V aa Move plate X amp Y to coordinates IY ESEE E Reference the plate Y axis again the indexing sensor 3 1 12 Mixing IMXONIILE 2 2 2 2 AR ai S gas Turn on plate mixer tttt time in seconds ld Kit Turn off mixer IMSPDSSSS etre eie eds Set mix speed duty cycle ssss duty 0030 is the default A larger number results in a slower mix speed IWSHIH EE Move wash head to home VT e Er Move wash head to coordinate zzzz Service Manual ELISYS UNO 47 106 3 1 14 Probe X Z Control IPRXH BEE Move probe X axis to home IPRZ
99. to the position in Rack 2 and aspirate nn position vvvv vol in uL s speed 0 9 Go to the position in Rack 2 and dispense nn position vvvv vol in uL s speed 0 9 NOTE The above commands will be automatically translated by the ELISYS UNO software into commands of the following form This allows the software to automatically accommodate the different racks that can be loaded into the instrument IGORKnyyyyXxxx ue IGRKAnyyyyxxxxzzzzvvvvSsaaaa IGRKDnyyyyXXXXZZZZVVVVSS 3 1 21 Washer Functions IWSETxxxx IWPRI Move probe to rack n rack yyyy rack pos xxxx probe X pos Goto rack and asp n rack yyyy rack pos xxxx probe X pos zzzz probe dip vvvv volume in mL s speed 0 9 aaaa air pocket in uL Goto rack and disp n rack yyyy rack pos xxxx probe X pos zzzz probe dip vvvv volume in mL s speed 0 9 Set the wash head depth to use when washing xxxx depth Prime the wash system one time Dispense v uL from wash head vvv volume per will in ul Prime the rinse system one time Aspirate on the plate ss start strip 1 12 ee end strip 1 12 d D for double 50 106 Service Manual ELISYS UNO IDSPSsseevww idees IASDSsseevvV errar 3 2 Error Messages Human Dispense on the plate ss start strip 1 12 ee end strip 1 12 vvv vol in uL Do an aspirate dispense on plate ss start strip 1 12 ee end
100. troduced into the system by rotating the shear type valve to connect between the 2 5mL syringe and the priming tube then drawing down on the syringe to aspirate prime fluid from the bottle A third port on the top of the valve is unused Volume pipetting is produced by displacements generated in two syringes 2 5 mL and 50 uL The 2 5 mL syringe is used for large reagent volumes and the 50 uL syringe is for volumes less than 30 uL The 50 uL syringe is attached to a T fitting and does not require a valve The syringes are connected by an interconnect tube between the valve 2 5mL and T fitting 50mL and this is coupled to the pipetting system by a feeder line between the T fitting 50mL and a coupled line into the heat block probe assembly See Figure 71 3 13 2 13 Photometer The photometer consists of a mechanism with its own electronics and additional calibration and analog to digital conversion circuitry on the Coprocessor PCB The mechanism contains the light sources filters detectors and electronics Light from four tungsten xenon lamps passes downward though four wells in the sample plate and the samples they contain Inside the sealed box under the read wells is a rotating filter wheel and four photodiode detectors The filter wheel contains eight interference filters of various wavelengths and is speed controlled to approximately 3 rotations per second under software control 36 106 Service Manual ELISYS UNO Hum
101. ver the fittings dip ends of tubing in isopropyl alcohol Be especially careful not to kink stretch or tension the tubing The replacement tubing should be routed in exactly the same place as the original Mounting Screws Valve Valve Pinch Tubing Bracket Do not loosen these screws The adjustment Plunger will be disturbed and Solenoid and the valve Spring may not operate properly Figure 4 10 1a Wash and Rinse Dispense Valve Tubing Replacement e Pinch Bracket Do not loosen Tubing these screws The adjustment Combo Block will be disturbed x Tubing Retainer and the valve may not operate properly Figure 4 10 1b Bleed and Control Valve Tubing Replacement Solenoid Service Manual ELISYS UNO 81 106 4 10 2 Wash Dispense and Rinse Dispense Valve Replacement e Set the power to OFF 0 e Refer to Figure 1 3 12 4 10 and 4 10 1a Locate tubing and valve of interest in diagrams Note where valve tubing make connection with fittings just above the valve and disconnect tubing e Remove dispense valve mounting screws from the valve needing replacement e Unsolder the wiring from the original solenoid and resolder to the replacement solenoid e Install replacement valve by installing the mounting screws e Cut the replacement tubing to fit e Replacement valves are pre adjusted and do not need further adjustment e Reconnect the tubing to fittings Push the tubing o
102. ver the fittings until seated Be especially careful not to kink stretch or tension the tubing The replacement tubing should be routed in exactly the same place as the original 4 10 3 Bleed or Control Valve Replacement Note Bleed valve must be continuous duty type solenoid and will be marked CONT e Set the power switch to OFF O e Refer to Figure 1 3 12 4 10 and 4 10 1a Locate tubing and valve of interest in diagrams Note where valve tubing make connection with fittings just above the valve and disconnect tubing e Hold the solenoid plunger away from the valve tubing as indicated by arrow and remove the tubing by pulling through opening Do not loosen these screws The adjust ment will be disturbed and the valve may not operate properly Tubing Opening Replacement Mounting Screws Figure 4 10 3 Replacement Bleed Valve Mounting 82 106 Service Manual ELISYS UNO Human Remove the two screws which secure the defective valve Replace with new valve assembly by securing the assembly with the two supplied replacement mounting screws and lock washers Replacement valves are preadjusted and do not need further adjustment e Cutthe replacement tubing to fit Hold the solenoid plunger away from the valve bracket and insert the tubing between the plunger and the bracket while routing the tubing through the holding slots in the valve bracket Reconnect the tubing to fittings Push the tubing over the f
103. zed protocols by selecting displayed menu options from a Microsoft Windows 1 software program This open system can be programmed to perform any of the colorimetric biochemistry assays or EIA assays that can be handled using the volumes temperatures and wavelengths provided It has many possible applications in clinical and veterinarian testing environmental testing food and water analysis life Science research and may also be used in production processes involving micro volume dispensing diluting incubating reading washing and so on Service Manual ELISYS UNO 1 106 1 2 Warnings and Precautions N For in vitro diagnostics use WARNING 1 2 1 Intended Use The ELISYS UNO is designed for use in processing general chemistry and enzyme linked immunosorbent assays ELISA or EIA including clinical diagnostic assays requiring multi step washing rinsing and soaking This general purpose instrument is intended to be used by laboratory professionals who are capable of selecting the appropriate features and options for each specific clinical application e Some diagnostic assays utilize materials which are potentially biohazardous e Always wear protective apparel and eye protection while using this instrument e Always operate the instrument with the aerosol shield lowered e Do not use the instrument in a manner not specified by the manual or the protection provided by the instrument may be impaired e P

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