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Optocomp Service Manual

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2. eere trennen 56 56 OPTOCOMP Parts eerte tette tette tete tete 56 OPTOCOMP I and Common 57 INJECTOR SPARE PARTS 4 04 e oem tm ts 57 SCHENUSTICS e Cert 58 Document no 8000 0006 00 Revision B Page 3 64 OPTOCOMP Service Manual SAFETY SYMBOLS The lightning flash with arrowhead within a triangle is intended to tell the user that parts inside the product are a risk of electrical shock to persons The exclamation point within a triangle is intended to the user to the presence of important operation and servicing instructions in the literature accompanying the device CAUTION Disconnect power cord before removing cover See user s manual prior to servicing Document no 8000 0006 00 Revision B Page 4 of 64 OPTOCOMP Service Manual INTRODUCTION The OPTOCOMP I and II Luminometers are stand alone photon counting instruments used in measuring light emitted from bio or chemiluminescent reactions The major difference between the two instruments is that the sample tubes are hand loaded one at a time into the OPTOCOMP I while the OPTOCOMP II can process 250 samples automatically in sequence The emitted photons
3. 40 REPLACING KEYBOARD I eese 40 REPLACING KEYBOARD PCB IL eese 41 REPLACING KEYBOARD PCB OPTOCOMP IB AND 4 REPLACING PRINTER ASSEMBLY OPTOCOMP I seen 42 REPLACING THE PRINTER ON THE 42 REPLACING THE PRINTER IN THE OPTOCOMP IB AND 43 REPLACING LID SEALS OPTOCOMP I AND IB eere 44 TONER TID SEALS ee ub Rae S aod de er M 44 Document no 8000 0006 00 Revision B Page 2 of 64 OPTOCOMP M Service Manual UPPER TID SFA Dri 44 LID SEALOPTOCOMPI n kamuua 45 REPLACING INJECTORS De E COM Meam 46 OPTOCOMP I AND II WITH OUTBOARD INJECTORS 46 n BA d tad x ERE not Posen ce 47 E 49 REPLACING OPTOCOMP TUBE 5 5 4 2 102011000000 50 REPLACING TUBE 5 5 4 4 2 5 2 2 21 000000000001 5 REPLACING OPTOCOMP CASSETTE 5 00 44 100 001 000000004 52 FRONT CASSETTE DRIVE wwwwwwwmmwmmanmmmammwm mm 52 REAR CASSETTE 00 402222 2 2 6 mwm 54 OPTOCOMP IIB FRONT CASSETTE DRIVE erret 55 OPTOCOMP RECOMMENDED SPARE PARTS
4. e 1 C22 214 1 5 1 1 RO Hoo Pg 5 53 RBZ 10 00 3 US 3 LEM Use RGZ Ps m i toe 358 212 110 Di gt 1476 1 vya 1 AA 6 1 0 5 OE 3 5 2 2 s _ 36 991 SHUTTER STALLED s fut SIUS 259 7 TSK co coo 4 2 155 c34 259 1 10 SK m a taa e 1 LG 8 2 5056556 See Note 3 _ _ Jie SHUTTER RUN 1 85 M SHUTTER DRIVE 2 gt m hs F04 F04 E 1 Ut YA SHUTTER 00 MOTOR Da US E See Note 3 5 2PM Ji2 8 SHUTTER DIR gt 2 5 id vs 12008 IL ENABLE 4 rA s See Note 2 u2 Ye 3 INDEX LOCRTOR 5 HC007 2 2 2K ODHMS 3 32k AAA AAA J12 12 R77 71 R101 13 00 R68 FROM 6 0 OHMS 2 7 R55 2 C27 R59 as FRONT STOP fu Cp oak HC00 1 011 Sect 3626250 See Note 3 135 il U3 Us S 5 2J12 FRONT INDEX 5 9 8 INDEX DRIVER 5 CASSETTE gt A 12UUR lt 5 GND L DRIVE Eo E E 2318 4 MOTOR iN E 5 BN Heo P 1 YA Nto LLL 5312 5 E HC02 C 2 5 REAR R27 14414 44 CASSETTE PH MEG See Note 3 DRIUE gt gt 12 6 MOTOR dd EN ju4 13 12UUR lus FRONT REAR 12 JHC02 09 2 Sa ALL RE
5. 12 Check the High Voltage setting of the replacement PCB as follows Connect a voltmeter between the J2 pins 1 and 3 on the PCB Connect a test lead Ball Clip to Alligator Clip etc between U 16 pin 6 and ground This is necessary due to the fact that the high Document no 8000 0006 00 Revision B Page 33 of 64 OPTOCOMP M Service Manual 13 14 15 16 17 18 voltage is not tumed on unless the lid is closed and the safety shutter is open Grounding U16 pin 6 defeats this feature Set the voltmeter to a range that will accept up to 1500 VDC Turn the OPTOCOMP on and observe the voltmenter The high voltage setting should be 1100 VDC If necessary adjust R13 on the MAIN PCB until the voltage at J2 1100 VDC NOTE If the voltage is at or around 460 VDC and will not change when R13 is adjusted check the connection of the ground lead to U16 pin 6 Turn the OPTOCOMP off and remove the meter leads and the test lead connected to U16 Replace front panel using care to ensure that the pin for the lid latch mechanism fits into the slot on the lid latch plate Set the panel in place apply power to the OPTOCOMP and press the LID RELEASE button once or twice to make sure that the solenoid fires and the lid latch plate rotates Install all front panel screws Install front shroud and screws MAIN PC BOARD REPLACEMENT OPTOCOMP I B AND II B 1 Zi wi oU Er E 10 Press the LID RELEASE button and open the lid OPTO
6. 901421 60035 E 5118 INJECT2 Bile UZ oa XTRL EXTRL vig 14 aag MESI SA mes 29 4 laz viz 16 8012 204 2 10 58 2 via 18 ADI 505 12UUR 1 50 11 yai 9 8011 50 029 Ritt 8175 15 2 acto F160 ub JILA ate 5 15 aas yo B By ET ME op INJECTS A15 12 3 AB ld 9 R119 F u27 fia EZ P24 1 ivo 9 TOK 05 64180 26 RAD 12 mo 10 ENT 054 1 TRESto gt 12VUR M13 55 816 5 hi 2 R144 5 4 pia 1 ive R115 24 1Y3 b gt 2 Sek RoHS 25035 1106 J12 040 12 1 2 ARESET ato ES 244 3 iC 2105 2 Hee 5 19165 ee 057 VYA TENDI OUT AB Hr Ge 9 5 12 H 3 17 ga 19UUR 4 12 1 DREQIN 20 8 vii ie _ac2 gt mH 9 8116 eteo ta cH EENABLECOUT AG H late LA its 1A 2 MBITAUIN 0555 4 615 viz E acs 50 33 6 VV 5 BUSREQ IN 2 18___804 dciza 1 JP15 5 o V az 1 S nOD aJe 05 37 SHEET 2 15 38 a uz 2 ace 4 dea 14 38 i pae vae SA n 2 vipi PAG 5 s 5U Rez ves 5 5 13 17 3 aco 12 82C55A 40 F510 yaa ES i Mm PT 10 SHEET 3 2 ate tout BL ace 2 o 140 0 _ ole R145 07 8022 Slo vep 2 pa S 475K GU D6 041 gt 2 i 07 n ji C42 C88 SQREF OUD 05 HC245 265106 PO
7. Turn the OPTOCOMP on and observe the voltmeter The high voltage setting should be 1100 VDC If necessary adjust R13 on the MAIN PCB until the voltage at J2 1100 VDC NOTE If the voltage is at or around 460 VDC and will not change when R13 is adjusted check the connection of the ground lead to U16 pin 6 13 Turn the OPTOCOMP off and remove the meter leads and the test lead connected to 016 14 Replace front panel Document no 8000 0006 00 Revision B Page 35 of 64 OPTOCOMP M Service Manual REPLACING THE DISPLAY OPTOCOMP I Tools required Philips screwdriver p pol QV Ure 59 10 12 13 14 15 Turn the off and remove the power cord from the wall socket Remove the top cover NOTE There is enough slack in the printer harness to allow the top cover to be placed on its side on the side of the instrument to the operator s left Remove the four screws holding the front panel Disconnect all wires and cables from the Front Panel to the Main PCB Remove the front panel Place the front panel upside down on a table or bench top Remove the two screws holding the display at the end near the red and black wires Remove the two flat head screws and plastic shoulder washers from the opposite end of the display Remove the display Install the new display 11 Install the two pan head screws through the mounting holes in the display near the red and black wires Install the two flat head sc
8. bellows etc the injectors If the injectors show no signs of leakage and are working satisfactorily replace the lid rear panel and front panel REPLACING OPTOCOMP II TUBE SENSOR Vo e pav 12 13 14 15 16 With power applied press the Lid Release button and open the lid Turn the off and remove the power cord from the wall outlet Remove the Front Shroud Remove the screws from Front Panel Disconnect the wires and cables from the Front Panel to the Main PCB and remove the Front Panel Disconnect the Lid Release Solenoid from the Main Harness Remove the 8 screws from the Upper Light Shield and remove the Upper Light Shield TURE Figure 5 Optocomp tube sensor Locate the Tube Present Sensor along the inside wall of the cassette housing Remove the two screws from the Tube Sensor Assembly Disconnect Tube Present Sensor Check the replacement Tube Sensor for binding etc and install if the plunger moves satisfactorily Replace Upper Light Shield Connect the Lid Release Solenoid Connect the Lid Sensor on Front Panel as well as the keyboard printer and display and install Front Panel NOTE Turn the power on at this point and press the Lid Release button to check that the Lid Release Mechanism works properly Replace Front Panel screws Replace Front Shroud Document no 8000 0006 00 Revision B Page 50 of 64 OPTOCOMP Service Manual REPLACING OPTOCOMP II
9. or both or none may be used with programmable delay timing KINETIC Up to 100 timed count intervals programmable from 0 02 to 650 seconds The time delay between count intervals is also programmable from to 3600 seconds Results may be reported as RLU values RLU vs time or both LUMINESCENT IMMUNOASSAY LIA Up to 10 Standard values may be entered used each with up to 10 replicates Timed counts may be programmed from 0 02 to 650 seconds The mean value and of up to 10 replicates is reported A menu of data handling routines including Ratio Point to Point and Spline Smoothing as well as others Document no 8000 0006 00 Revision B Page 8 of 64 OPTOCOMP M Service Manual CUTOFF Qualitative Sample RLU are compared to a Sample Value times a Cutoff Factor The reference may be either a Negative Reference or a Positive Reference Cutoff Factors are programmable Results are reported as Positive Negative or Equivocal relative to the Cutoff value s Complete programming flexibility is provided Operator safety has been a major consideration during the design and development of the OPTOCOMP I and OPTOCOMP II With proper use and care it will provide safe and reliable operation Misuse however could result in either damage to the instrument or serious personal injury to the operator CAUTION DANGEROUS VOLTAGES EZIST INSIDE THE INSTRUMENT USE CAUTION WHEN SERVICING This manual is intended for use by tec
10. us s ird i ag HPLT SLEEP 04 1096 1005227 05 PC6 ES 1 INATAB c LIR OUT D3 062 955 IDA PCS 2 1 CR30 J10 1 18 2 ya 82055 251 05 15 154148 46 R164 E m eum tes pt viue Dez ip ce pet I 2 DESEADA pcz HZ S o R162 410 7 4 Arao gt Slave 212 066 2 1 Roy pee 00123501 poo 6 S05 1410 A 959 43 16 pcs 065 5 25 00034 115 eeuF CTS0 215 15 05 WR 1 00 C1 051229 445 RD 5 jar 15 DoD 4 ari 8 0 14 11 6 2 6 CKA0 DREQU Ri4 Yl4 at CO 18 v MWEE 6 21 4 062 DG 5 iki PS aco 910 pa 22 VALKE 22 410 2 211007 TIIN 14 ad INTO 7_ 22 1509655 pig 6 D2 oTi 13 35 RST PEG 24 J10 4 511017 T2INGS RXAD INT1 22 12 001 Dit 7 Di GATEL 14 36 Ny 285 25 RTS0 INT2 E3 yaa DCO 8 50 22 J10 5 d 1 0 ycks E 025 akel zli 210 5 REIN ReOUT LS DSD0 cout Txs PE act 20 es FY z S 20 10152 11 7 4 16 RD Le 050 030 PB REDUTIZ TxAL 7 197 pares te Eza pet 9 2 OUT CKA TENDO EA 1 5 Nac a i va EE 1 E 1 1 OINXKS CISL 5 HC244 PE gt 7 111 7 2 049 3 D48 58 ce RXAL 25 ee 1 2 CR26 URLUE 1 TS eur 11 46 1 1N4148 J14 1 cB F miran HC04 NE 2 2574 er J oe
11. OPTOCOMP off and remove the power cord from the wall socket Remove the four screws from the outer shroud and remove the shroud Remove the three screws from the top of the front panel Remove the four flat head screws from either side of the front panel Lift the front panel slightly and disconnect the lid sensor Lift the front panel further and disconnect the harnesses running from the keyboard display and printer to the MAIN PCB Remove the front panel and place upside down on a table or bench top Remove the six Philips head screws holding the keyboard Remove the keyboard Install replacement keyboard NOTE It may be necessary to retain the ribbon cable from the original keyboard Replace six Philips head screws 13 14 Return the front panel to the base Connect the keyboard and display to the Main PCB J9 Keyboard J8 Display Ribbon Cable J13 Display Backlight Connect the lid sensor Replace flat head screws to the sides of the panel Replace the three screws to the top of the front panel Replace the front shroud REPLACING KEYBOARD PCB OPTOCOMP IB AND IIB l oa With power applied press the lid release button on the front panel OPTOCOMP II only Remove the two screws from the top of the front panel Lift the panel to a vertical position Disconnect the ribbon cable from the keyboard to the MAIN PCB Remove the six hex nuts from the backside of the keyboard NOTE It is necessary to remove
12. dropping the new seal into the opening at the top of the well chamber UPPER LID SEAL SLIT 4 UPPER LID SEAL 4 With power applied press the Lid Release button 5 Gently grasp the rubber Upper Lid Seal and pull down gently 6 Install new seal by gently sliding it onto the black nozzle in the lid NOTE The upper lid seal is slit to reduce the vacuum effect on the chamber Without the slit the lid may not open Make sure the slit in the new seal faces down when the lid is open Document no 8000 0006 00 Revision B Page 44 of 64 OPTOCOMP M Service Manual LID SEAL OPTOCOMP II 1 With power applied press the Lid Release button on the Front Panel and open the lid 2 Pinch the rubber lid seal gasket between you fingers and gently lift upward The gasket is not anchored and relies on a press fit to keep it in place 3 Install new Lid Seal Gasket by pressing it into the channel around the cassette housing Start from the middle of each side and work the material toward the corners to keep the material evenly distributed LID SEAL GASKET Document no 8000 0006 00 Revision B Page 45 of 64 OPTOCOMP Service Manual REPLACING INJECTORS Tools required Philips screwdriver 11 32 wrench or nut driver soldering iron OPTOCOMP I AND II WITH OUTBOARD INJECTORS 1 Switch the instrument off 2 Disconnect the Injector Cable from the rear panel of the OPTOCOMP 3 Disconnect the output lines from the injectors 4 R
13. ground lug from the PREAMP to change the PMT Document no 8000 0006 00 Revision B Page 29 of 64 Service Manual 9 10 11 12 13 14 15 16 17 18 19 20 Remove from the SHUTTER BLOCK assembly If necessary use a small pair of NEEDLE NOSE pliers to grip one of the pins on the PMT and using extreme caution lift straight up Check the replacement PMT to make sure that the felt packing is in place about an inch from PMT window NOTE Do not expose PMT to any excess ambient light as this will shock the PMT and cause the PMT to operate erratically for the first hour or so after power is applied Leave the rubber cover on the PMT until you are ready to install it into the SHUTTER BLOCK Install PMT Replace the PREAMP by lining up the pins on the PMT with the sockets on the If necessary gently rotate the PREAMP to align it with the mounting holes on the ring Install the two screws which secure the PREAMP to the mounting ring using care to assure that the two nylon spacers are installed on the back side of the PREAMP Install the PREAMP cover bar and two screws Replace rear panel Replace top cover Restore power to the OPTOCOMP I From the MAIN NEMU press the UTILITIES key on the keypad Press 11 ENTER RESUME Set the PMT FACTOR to 1 and press ENTER Whenever the PMT is replaced it is necessary to reset the PMT factor The PMT factor is det
14. groundlug to the PREAMP mounting plate 8 Remove the two screws holding the PREAMP CAUTION There are two nylon spacers on the back of the PREAMP which will fall off when the screws are removed C d 9teo otra YA 5 tt ai nf REMOVE SCREWS 9 Carefully remove the PREAMP NOTE The PREAMP is pressed onto the PHOTOMULTIPLIER TUBE or PMT It may be necessary to gently wiggle or rock the PREAMP until it is free of the PMT 10 Check the Revision Level of the replacement PREAMP to make sure that it is at least the same or higher than the one being replaced The Revision Level is printed in the upper right hand corner of the component side of the PREAMP 11 Install the new PREAMP by carefully pushing it onto the pins of the PMT NOTE The PMT is keyed to prohibit incorrect connection 12 Replace the groundlug onto the stud and tighten the nut with the 5 16 wrench 13 Connect the three cables to the PREAMP NOTE The coaxial cable with the three position connector should be plugged into J3 on the PREAMP 14 It is good practice at this point to check that the threshold voltage is properly set on the PREAME Before replacing all of the covers on the instrument connect the voltmeter between ground and U4 pin 2 on the PREAMP This is easily accomplished by connecting to R29 which is located next to the potentiometer R28 R29 is the middle resistor of the three located on the right of R28 Carefully apply power
15. pus 19 114 2 244 505 R166 Jt 10K 13106 2 aL MWE FROM 5 4 E SHUTTER STOLLED tre SHEET 2 KEYBD J RL mE 10 FROM SHEET 2 dei D um gt a gt sue tob o COUNT DONE INTERRUPT soe E R123 2 5 yX BEEPER MC 8 2 416 7 5 5 ALL RESISTORS 1 46 1 METAL FILM UNLESS OTHERWISE NOTED RNI corp 106 4 50 UNLESS OTHERWISE SPECIFIED ALL DIMENSIONS ARE IN INCHES TOLERANCES ARE FOLLOWS FRACTIONS DECIMALS ANGLES INSTRUMENTS INC 1 32 01 005 r gt MATERIAL 28 2000 28 2000 APPROVED DESIGNED BY BZ SCALE DRAWN BY ZM FINISH PART NAME TITLE REVISION SPECIAL NOTE APPLICATION DRAWING NUMBER 5 0700 0011 00 1 of 4 C Program Files ACAD2000 DRAWINGS SCHEMATICS 0700 0011SH1REVG3 dwg amp C0 152 00 70 LO FROM SHEET 1 FROM SHEET 1 8255 3CS gt 8255 265 gt RD gt WRe RESET JS J5 J5 J5 J5 J5 INDEX LOCATOR d us CASSETTE RERRe CASSETTE FRONT T5 R106 0 50 1 2 INDEX LOCATOR IL ENABLE TO FRONT RERR INDEX 4 FRONT STOPs 2 R142 4 7K 1 5U dA J14 0 FROM SHEET 1 J14 10 __ 22 CoB ts D5 lt 5 Dee eis FROM SHEET 1 P TO TP 5 5 oM JP1 e 42 20 4 7F 15 14 15 12 11 27 TO SHT 1 4 29 ALL RESISTORS
16. s Manual for instructions on running Q A to assure proper operation PMT REPLACEMENT OPTOCOMP I Part Number 0300 0048 00 Tools required Philips screwdriver 1 and 2 point Tritium calibration source 1 2 o gt Turn the OPTOCOMP I off and remove the plug from the wall receptacle OPTOCOMP I ONLY Remove the Top Cover by removing the four screws two on either side and lifting straight up See section Removing Covers Remove the 5 screws holding the rear panel and set it aside OPTOCOMP IB ONLY Remove the two screws securing the front panel located on the back lip of the front panel toward the rear of the instrument Pull the front panel toward you Itis hinged and will stay in the upright position Remove the screws holding the rear panel Lift the panel straight up and remove it from the instrument Document no 8000 0006 00 Revision B Page 28 of 64 OPTOCOMP M Service Manual i CH 6 Remove the two screws from the PREAMP COVER Remove the BAR and COVER from the PREAMP 7 Remove the two screws holding the PREAMP CAUTION There are two nylon spacers on the back of the PREAMP which will fall off when the screws are removed R EMOVE sc REWS 8 Carefully remove the PREAMP NOTE PREAMP is pressed onto the PHOTOMULTIPLIER TUBE or PMT It may be necessary to gently wiggle or rock the PREAMP until it is free of the PMT Place the PREAMP aside Itis not necessary to disconnect the wires or the
17. the washers and plastic spacers at this time Document no 8000 0006 00 Revision B Page 41 of 64 OPTOCOMP M Service Manual 6 8 9 10 Remove the keyboard NOTE Use care not to lose the spacers from the front side of the keyboard Install new keyboard making sure that there are spacers between the front panel and the keyboard Install spacers washers and hex nuts Connect the keyboard to the main PCB using the ribbon cable Close the front panel and replace the two screws REPLACING PRINTER ASSEMBLY OPTOCOMP I Tools required 1 point screwdriver 4 nut driver or socket pod oon 10 11 12 13 Turn power to instrument off remove power cord from wall receptacle Remove the Top Cover Remove the screws from the Front Panel Lift the Front Panel and disconnect the Printer Harness from the Main PCB and the Paper Advance Button Turn the Top Cover over and remove the six hex nuts securing the Printer Assembly Remove the Printer Assembly Install new Printer Assembly and replace hex buts and washers Connect the printer harness to the Main PCB 26 for 3 wire printer power J7 for printer data wire Replace Front Panel Replace Top Cover Install a roll of paper Press the Paper Advance button while applying power to the instrument to initiate a self test During the self test the display will read ATTEMPTING TO INITIALIZE PRINTER The printer will conduct a
18. useful for troubleshooting and repairing the Luminometers To access these utilities press UTILITIES enter the number of the utility you wish to access press ENTER At this point the instrument will beep signaling an improper keystroke press RESUME to display the utility OPTOCOMP I hidden utilities are listed below SERIAL NUMBER 10 FACTOR 11 INJECTOR TYPE 12 SENSOR STATUS 13 TWO POINT ADJUSTMENT 14 DISPLAY EDITED FLAG 15 The HIDDEN UTILITIES for the OPTOCOMP II are listed below HIDDEN UTILITY MENU 11 SERIAL NUMBER 12 PMT FACTOR 13 INJECTOR TYPE 14 SENSOR STATUS 15 TWO POINT CALIBRATION 16 DISPLAY EDITED FLAG 17 CUBE TYPE 18 DEFAULT DISPLAY NAME 19 DISPLAY OVERLOAD FLAG 20 HIDDEN UTILITY MENU Lists the numbers for the following choices 11 17 SERIAL NUMBER changes the serial number in the system memory This number is printed on Q A printouts as well as headers for all tests conducted on the instrument PMT FACTOR Basically the same as CALL FACTOR except it is not user selectable This is a method of normalizing the photomultplier tube INJECTOR TYPE Sets the system up for either an MGM type injector or a VALCOR type injector This adjusts the cycle time of the pulse sent to the injector by the CPU This should always be set to MGM SENSOR STATUS Gives a visible readout of the status of the sensors inside the instrument see belo
19. 0 0011 00 MGM INSTRUMENTS e 62 of 63 OPTOCOMP M Service Manual I UJ UI VT 1 6 ma Pici Pl 12 19 997 49494949 UU O706 DOA OPTeC ome AnP P C 4 SCHERATIC
20. 1 4W 17 METAL FILM UNLESS OTHERWISE Romecs y FROM 5 1 TO DISPLAY TO PRINTER 2 spe J7 1 PRINTER RCK J 10 15 PRINTER BUSY 177 11 oTP14 TO KEYBOARD KEYBD gt SHEET 1 MGM INSTRUMENTS INC DATE NOV 28 2000 PART NAME TITLE MAIN PC BOARD APPLICATION DRAWING NUMBER 0700 0011 00 C Program FilesVACAD2 HUe LU REVISIONS DESCRIPTION DATE APPROVED ELEASED PER ECN 102535 5 8 95 2 ELEASED PER ECN 102879 Ji 5 C9 20 TANT ee 55 470 T 25 25 25 12008 Ji 9 C SUPPLIES AGND t ISOLATED TP19 DOO0 DRAWINGS SCHEMATICS 0700 0011SH3REVG3 dwg lt J3 2 3 MTR ADJ R16 gi 20M 172 W LANE AAA 120 SHIELD OF IS GROUNDED PINS 1 5 UE dU UO W 2 lAAA 2 _ doc 5 1 1000 9 2 R22 R10 16 1 c40 1 cis 1 C16 1 C17 1 Ciz 4 9 AP 27 nU otur 5 3 0KU 0 5 0ku 3 0KU 3 0ku Ja C7 Sf 2414 C6 00220 R9 E 1 1 470 2 5 H V P S UNUSED GATES CONNECTED EXTERNALLY 1 2 2 15 3 E R168 1000PF 2iji 2 T GROUND PL
21. 5 Install the two flat head screws and shoulder washers to the other standoffs in the front panel taking care to mount the washers with the shoulder facing the front panel 16 Connect the cables from the keyboard and display to the proper places on the MAIN PCB J9 keyboard J8 display ribbon cable J13 display backlight 17 Connect the lid sensor 18 Replace front panel 19 Replace front shroud Document no 8000 0006 00 Revision B Page 37 of 64 OPTOCOMP M Service Manual REPLACING THE DISPLAY OPTOCOMP IB AND IIB 1 With power applied press the Lid Release button and open the Lid OPTOCOMP IIB only 2 Turn off power to the instrument and remove the plug from the wall socket Remove the two screws from the top of the front panel 4 Liftthe front panel and place it in a vertical position 2 Figure 2 Optocomp IB front panel rear view Bh Disconnect the display ribbon cable and the two wore backlighting harness from the Main PCB Remove the four screws from the display Remove the display Install the new display Replace the four mounting screws 10 Connect the cables from the keyboard and display to the proper places on the MAIN PCB J9 keyboard J8 display ribbon cable J13 display backlight 11 Close the front panel and replace the two screws at the top of the front panel Document no 8000 0006 00 Revision B Page 38 of 64 OPTOCOMP M Service Manual REPLACING PUSH BUTTON ASSEMBL
22. ANE POWER FAIL SENSE 70 SHEET 1 5U URZ LM2576T 5 50 2 R450 5U 1 4 4 1 de 14 J 1 5 220UH C78 C68 C67 c93 CoB 15 1 UF ALAR 2 120 1 1 1 1 1 1 1 1 C74 C73 C72 C71 70 C69 C80 UF 169 2 2 2 2 2 2 ALL RESISTORS 1 44 1 FILM UNLESS OTHERWISE NOTED MOUNTING HOLES 888 8 UNLESS OTHERWISE SPECIFIED ALL DIMENSIONS ARE IN INCHES TOLERANCES ARE FOLLOWS FRACTIONS DECIMALS ANGLES 1 32 xei 01 005 iT M G M INSTRUME MATERIAL DATE NOV 28 2000 APPROVED SCALE FINISH PART NAME TITLE SPECIAL NOTE APPLICATION MAIN OPTOCOMP 5 INC DESIGNED BY BZ DRAWN BY DRAWING NUMBER 0700 0011 00 ZM REVISION G 3 SHEET 3 of 4 1 Short R83 0 Ohms for UC3705T If using SG3635 then don t use R83 Place jumper to ground by adding jumper from 07 pin 11 to U7 12 R83 opened Add 3 39K RESISTOR R78 across U7 pins 15 ond 16 E 12UUR 12UUR 2 2K 0 OHMS 3 32K A A M AV AN me NN e ie TO 2 lt P 2 5895 R100 R99 R78 38 5 RBS
23. B TUBE SENSOR With power applied press the lid release button and open the lid Turn the power off and remove the power cord from the wall outlet Remove the two screws from the top of the Front Panel Rotate the Front Panel to the open position Disconnect the Lid Release Solenoid from the Main Harness Remove the 8 screws from the Upper Light Shield and remove the Upper Light Shield Locate the Tube Present Sensor along the inside wall of the cassette housing Remove the two screws from the Tube Sensor Assembly Disconnect Tube Present Sensor Check the replacement Tube Sensor for binding etc and install if the plunger moves satisfactorily 11 Replace Upper Light Shield 12 Connect the Lid Release Solenoid 13 Connect the Lid Sensor on Front Panel NOTE Turn the power on at this point and press the Lid Release button to check that the Lid Release Mechanism works properly 14 Close the front panel and replace the two screws to the top of the front panel SO Qo ob vc Unde paces Document 8000 0006 00 Revision Page 51 64 OPTOCOMP M Service Manual REPLACING OPTOCOMP II CASSETTE DRIVES FRONT CASSETTE DRIVE With power applied press the Lid Release button and open the lid Remove the Cassettes from the right side of the Cassette Housing Turn the OPTOCOMP off and remove the power cord from the wall outlet Remove Front Shroud Remove Front Panel Remove Upper Light Shield Remove Lower Light Shiel
24. CKGROUND LIMIT allows the user to set the maximum background luminescence prior to reagent injection Programmable from 0 to 999 NEXT MENU changes the display to selections 6 through 9 CAL FACTOR allows the user to normalize the instrument response by applying a multiplication factor Valid entries range from 063 to 2 0 COUNT DELAY allows the user to select a delay time in seconds prior to initiation of a count sequence Valid entries are to 10 seconds REPORT MODE allows the user to select the on board printer or the RS 232 port for data output FIRST MENU returns the user to the first UTILITIES display selections 1 5 The OPTOCOMP II differs slightly in the UTILITIES MENU It is as follows 1 OPERATOR NAME same as 2 DATE TIME same as I 3 Q A same as OPTOCOMP I 4 MOVE CASSETTES allows the user to position the cassettes using the internal motors of the instrument TAB INITIALIZE is also performed using this function placing the cassette drive tabs in the home position 5 NEXT MENU changes the display to selections 6 10 6 CAL FACTOR same as Document no 8000 0006 00 Revision B Page 11 of 64 OPTOCOMP M Service Manual 7 BACKGROUND LIMIT same as OPTOCOMP I 8 COUNT DELAY same as 1 9 REPORT MODE same as OPTOCOMPI 10 FIRST MENU returns user to the first five selections 1 5 SERVICES UTILITIES The HIDDEN UTILITIES are
25. COMP IIB only Turn the power off and remove the plug from the wall socket Remove the two Philips head screws from the top of the front panel and lift the front panel to a vertical position Disconnect all wires and cables from the MAIN PCB Remove seven Philips head screws securing the MAIN PCB Remove the MAIN PCB Check the replacement PCB for matching revision level NOTE It may be necessary to retain the EPROM S from the original PCB for use in the replacement Install new PCB into the base and replace the seven screws Replace the wires and cables to their respective plugs as follows JI AC power from main transformer 12 High voltage cable from PREAMP J3 5vdc for PREAMP 14 Signal cable from PREAMP 15 40 connector from sensors 16 Printer power J7 Printer data cable 18 Ribbon cable from display 19 Ribbon cable from keyboard 5 232 harness J11 Injector harness J12 Motor harness Check the High Voltage setting of the replacement PCB as follows Connect a voltmeter between the J2 pins 1 and 3 on the PCB Connect a test lead Ball Clip Alligator Clip Document no 8000 0006 00 Revision B Page 34 of 64 OPTOCOMP M Service Manual etc between U 16 pin 6 and ground This is necessary due to the fact that the high voltage is not turned on unless the lid is closed and the safety shutter is open Grounding U16 pin 6 defeats this feature 11 Set the voltmeter to a range that will accept up to 1500 VDC 12
26. D Injector Line Input Blue Injector Line Input Red Injector Line Dispense Blue Injector Line Dispense Red Fuse YA mp Slo Blo Cassette Extraction Tool Revision B Page 56 of 64 OPTOCOMP M Service Manual MGM Part Number 6110 0006 00 0112 0132 00 0110 4029 00 0110 4018 00 0110 4131 01 0112 0131 00 0112 0146 00 0800 0001 00 Description Lid Sensor Shutter Rack Assembly Shutter Cover 14 inch Shutter Cover Vanch Lid Latch Plate Shutter Motor Assembly Autotrol Shutter Motor Assembly Micreo Mo Operator s Manual OPTOCOMP I and Common Parts MGM Part Number 0700 0017 00 9100 0004 00 0700 0011 00 0112 0115 00 0112 3113 00 0112 0001 00 0112 0002 00 0112 0142 00 0112 0003 00 0300 0016 00 0300 0068 00 9000 0004 00 0111 3015 00 0200 0018 00 9100 0004 00 INJECTOR SPARE PARTS MGM Part Number 0112 0006 00 0112 0147 00 0112 0007 00 0112 0007 01 0200 0037 00 0200 0038 00 0112 0143 00 0200 0049 01 0200 0049 02 Document no 8000 0006 00 Description Keyboard Assembly Keyboard Overlay Main PC Board Assembly Pre Amp Board Assembly Photomultiplier Tube Printer Assembly LCD Display Main transformer Assembly Push Button Assembly lid Release and Paper Advance Power Entry Module Line Cord Thermal Printer Paper Paper Spool Foot Keypad Overlay Description Bellows Assembly Solenoid Assembly Injector Pump Assembly 200 300uL Injector Pump Assembly 50 199uL Valve Hitting Re
27. EASE key usually has to be struck repeatedly for this to happen as the chances of interrupting the CPU with a LID RELEASE request at the moment it is checking the shutter position are small This issue will be fixed in future software releases pA uus paru Qo Document no 8000 0006 00 Revision B Page 19 of 64 OPTOCOMP M Service Manual ADJUSTING OPTOCOMP I TUBE SENSOR The tube sensor protects the OPTOCOMP from injecting reagents into an empty sample chamber 1 2 3 With power applied Press Lid Release button Turn the power off and remove the power cord from the wall outlet Remove the Top Cover and Front Panel OPTOCOMP I or remove the two screws at the top of the Front Panel OPTOCOMP IB Do not disconnect the cables from the Front Panel Using a test lead ball clip alligator clip etc connect the tab of Q 7 on the Main PCB to ground This will cause the Tube Present LED to be continuously Restore power to the OPTOCOMP CAUTION Dangerous voltages exist inside the instrument Use extreme caution Using a voltmeter or oscilloscope measure the voltage at JP 11 to determine the output state of the Tube Present sensor A logic level low volts indicates that the sensor is turned on This is the state with no tube in the well Adjust R 148 on the Main PCB counterclockwise until the logic state at JP 11 changes to logic high level 4 5 VDC This is the proper stat
28. OPTOCOMP M Service Manual OPTOCOMP SERIES of LUMINOMETERS SERVICE MANUAL MGM Instruments Inc 925 Sherman Ave Hamden CT 06514 USA Phone 203 288 3523 Toll Free in US 800 551 1415 Fax 203 288 2621 E mail sales 9 mgminstruments com Web site http www mgminstruments com Document no 8000 0006 00 Revision B Page 1 of 63 OPTOCOMP M Service Manual SAFETY SYMBOLS 4 INTEODUCLTION aki 5 FRONT PANEL CONTROLS HI 6 THEORY OF OPERATION eU Ie T PREAMPEIFHER RE RSS ages 7 PMT SHUTTER ASSEMBLY amp PROTOCOLS 8 DATAS c AA on AA Ka za 8 ii na 8 LUMINESCENT IMMUNOASSAY amp CUTOFF 2 21 27 2 2 2000 0000000000000000000000000040 0 IA 9 SPECIFICATIONS it e dd p 10 MOTD UTES epo tuens pepe etiim eat MM e 11 SERVICES UTILITIES ii kaa kazia E e eda tu tas o i ape Mass RU UE 12 TROUBLESHOOTING Gute te Rud 15 Trouble shooting shutter Sta
29. SISTORS 1 4W 17 METAL C Program Files ACAD2000 DRAWINGS SCHEMATICS 0700 0011SH4REVG3 dwg FILM UNLESS OTHERWISE NOTED UNLESS OTHERWISE SPECIFIED ALL DIMENSIONS ARE IN INCHES TOLERANCES ARE FOLLOWS FRACTIONS DECIMALS ANGLES 1 32 01 005 T MATERIAL FINISH SPECIAL NOTE REVISIONS DESCRIPTION DATE APPROVED RELEASED PER ECN 102535 5895 1 RELEASED PER 102542 G 3 RELEASED PER 102879 BM SS 2 52 7 Note 2 Short R68 0 Ohms for 0 37057 Uf using SG3635 then don t use R68 Place jumper to ground by adding Jumper from 110 pin 11 to U10 pin 12 Leove R68 opened Add 3 32K RESISTOR R101 across 010 pins 15 ond 16 Note 3 When installing UC3705T into circuit board ot positi U8 U9 U11 or 044 swap pins 2 ond 4 Apply tubing te of these pins to prevent shorts See typical schematic below 0 3705 5 4 e 1 3 MGM INSTRUMENTS INC M PART NAME TITLE M I N p C B R D 2 5 4 of 4 APPLICATION OPTOCOMP DRAWING NUMBER 0700 0011 00 OPTOCOMP M Service Manual OW 8 3 mr Soe m Bow M XE m is B ap id poU wm ya amp 0 88 s YA B m 8 Sex 9 fe Document r REU F CPU BD P N 041
30. Y OPTOCOMP I Tools required Philips head screwdriver 4 wrench or nutdriver gee qos 9 10 11 12 13 Turn the off and remove the power cord from the wall socket Remove top cover Remove screws from Front Panel Disconnect all wires and cables from the Front Panel Remove Front Panel Figure 3 Optocomp IB Pushbutton assembly Remove the four hex nuts holding the push button mounting plate Remove the push button plate Install replacement push button assembly NOTE It may be necessary to retain the red and white buttons switch covers from the original assembly These are simply pressed on and can easily be removed by gently pulling on them Replace hex nuts and washers Place front panel gently into the base Connect the push buttons to the proper harnesses lid release to motor harness paper advance to the printer harness Install screws into front panel Replace top cover REPLACING PUSH BUTTON ASSEMBLY OPTOCOMP II l With power applied press the lid release button on the front panel and open the lid 2 Tum the OPTOCOMP II off and remove the power cord from the wall socket See section Removing covers Remove the FRONT SHROUD by loosening the four Philips head screws two on either side Remove the Front Panel by removing the three Philips head screws from the top of the Front Panel and the four flat head screws from either side of the Front Panel and lifting the p
31. ace Front Panel 14 Replace Front Shroud 15 Restore power and run the printer self test as described above REPLACING THE PRINTER IN THE OPTOCOMP IB AND IIB 1 With power applied press the lid release button and open the lid OPTOCOMP II ONLY Turn off the instrument and remove the power cord from the wall receptacle Remove the two screws from the top rear of the Front Panel Hinge the Front Panel to an open position Disconnect the Printer harness from the Main PCB Disconnect the Paper Advance button Remove the four hex nuts from the printer tray Remove the printer It may be necessary to retain the paper cover or door from the original printer assembly At this point remove the four screws which secure the Hinge Plates to the Paper tray and install the door on the new printer assembly 9 Install the new printer assembly 10 Connect the Printer Harness and Paper Advance button 11 Close the Front Panel and replace the two screws 12 Restore power to the instrument and test as described above po e sb Document no 8000 0006 00 Revision B Page 43 of 64 OPTOCOMP M Service Manual REPLACING LID SEALS OPTOCOMP I AND IB LOWER LID SEAL With power applied press the lid release button 2 Gently grasp the rubber gasket of the Lower Lid Seal and lift straight up NOTE The Lower Lid Seal is considered to be an assembly The black plastic ring is permanently attached 3 Install new lower lid seal by
32. an external computer The OPTOCOMP may also be programmed from an external source Document no 8000 0006 00 Revision B Page 5 of 64 OPTOCOMP M Service Manual FRONT PANEL CONTROLS START START a programmed protocol PROGRAM Edits protocol parameters LIST Lists protocol parameters UTILITIES Calls the UTILITIES menu INTIALIZE Primes injectors and injector lines RESUME RESUMES an interrupted protocol STOP Interrupts a protocol ENTER Accepts a displayed value Please consult the OPERATOR S MANUAL instructions for programming and normal operation Document no 8000 0006 00 Revision B Page 6 of 64 OPTOCOMP M Service Manual THEORY OF OPERATION The energy released as a result of the oxidation of certain luminescent molecules generates one or more photons of light The OPTOCOMP Luminometers are able to detect these individual photons by means of a highly sensitive PHOTOMULTIPLER TUBE PMT Figure 1 is a schematic diagram of a photon counting PMT 11 DYNODES GAIN 10 Figure 1 Photon counting photomultiplier tube The photons generated in the sample tube are collected by the photocathode surface on the back of the PMT faceplate This surface is formulated to generate free electrons in vacuum environment and reaches maximum sensitivity in the spectral region of luminescence blue The PMT consists of a series of dynodes each at a higher voltage The negatively charged electrons are attracted to the higher potential of
33. anel straight up Disconnect all cables and wires from the Front Panel Document no 8000 0006 00 Revision B Page 39 of 64 OPTOCOMP M Service Manual SO 00S 10 11 Place the front panel upside down on a table or bench top Remove the four hex nuts holding the push button mounting plate Remove the push button plate Install replacement push button assembly NOTE It may be necessary to retain the red and white button switch covers from the original assembly These are simply pressed on and can easily be removed by gently pulling on them Replace hex nuts and washers Reconnect all cables and wires to the Front Panel 12 13 Replace Front Panel screws Replace Front Shroud REPLACING PUSH BUTTON ASSEMBLY OPTOCOMP IB AND 1 8 9 10 11 With power applied press the lid release button and open the lid Turn the off and remove the power cord from the wall outlet Remove the two screws from the top of the front panel and lift the Front Panel to a vertical or open position Disconnect the Lid release button from the main harness and the Paper Advance button from the printer harness Remove the four hex nuts holding the push button mounting plate Remove the push button plate Install replacement push button assembly NOTE It may be necessary to retain the red and white buttons switch covers from the original assembly These are simply pressed on and can easily be removed b
34. are detected by a photomultiplier tube PMT and converted into electrical pulses The photon pulses counted are directly proportional to the amount of light emitted by the reaction are displayed as Relative Light Units RLU The resulting RLU are stored in memory for further data reduction and may be printed out for a permanent record A measuring sequence is initiated by selecting a protocol inserting the sample tube s into the counting chamber and closing the lid The OPTOCOMP II will automatically move the tubes into the counting position An internal computer controls any injections counts the RLU analyzes the data and displays and prints the results Up to 30 protocols may be programmed stored in non volatile memory and recalled at any time to run a test Programmable parameters include of test Cutoff LIA Kinetic Raw Data Protocol name Alphanumeric printout for positive test ID Injectors used None A only B only A then B Types of tubes Number of replicates of References Standards Controls and Sample tubes Count time Programmable from 0 02 to 60 seconds Data reduction Several different algorithms are available Consult the Operator s manual data is printed on the built in thermal graphics printer Kinetic study and LIA results may be plotted and the data displayed The RS 232 data communication port is b directional and may be used to transmit results to or receive control signals from
35. d ip cM Figure 6 Optocomp II front view front panel removed Document no 8000 0006 00 Revision B Page 52 of 64 OPTOCOMP Service Manual 8 Loosen the two hez nuts on the Cassette Drive Motor Bracket and rotate the bracket to provide slack in the drive belt e a CASSETTE 34 DRIVE MOTOR 9 Remove the two flat head screws from the right front of the Cassette Housing NOTE It may be necessary to remove the PMT to gain access to the center most screw The nozzle block should not be removed due to the fact that the aim of the injector lines is critical REMOVE THESE SCREWS 10 Disconnect the Tab Sensor PCB from the Main Harness Document no 8000 0006 00 Revision B Page 53 of 64 OPTOCOMP M Service Manual 11 Remove the Cassette Drive Mechanism 12 Check the replacement Cassette Drive Mechanism for freedom of travel etc connect the Tab Sensor PCBs and install if satisfactory 13 Replace the two flat head screws in the cassette housing 14 Check the Drive Belt for proper position of the sprockets 15 Pull the Drive Motor Bracket to remove any slack in the belt and tighten the two hex nuts on the Motor Bracket 16 Reconnect the Front Panel at this point and restore power to the instrument 17 From the Main Menu press Utilities 4 Enter to access the Cassette Motion Utility 18 Press to initialize the cassette drives Check to ensure that the tabs on the front drive are parke
36. d and not exposed in the cassette housing 19 From the Main Menu press 1 ENTER to cycle cassettes and 0 ENTER for Front Cassette first to check the operation of the new cassette drive mechanism If the drive is satisfactory turn the power off and disconnect the power cord from the wall outlet once again 20 Replace the upper and lower light shields 2 Reconnect the Lid Sensor Display Keyboard and Printer and install front panel Verify that the lid release mechanism works properly by either manually rotating the lid release or applying power and pressing the Lid Release button 22 Replace Front Panel Screws 23 Replace the Front Shroud REAR CASSETTE DRIVE With power applied press the Lid Release button and open the lid 2 Remove the Cassettes from the left side of the Cassette Housing 3 Turn the OPTOCOMP off and remove the power cord from the wall outlet 4 Remove the three screws from the lid at the rear of the Cassette Housing 5 Remove the 6 screws holding the rear panel and remove the Rear Panel 6 Remove the Lid Assembly by loosening the four hex nuts on the inside of the rear of the instrument and lifting straight up 7 Remove the rear light shield 8 Remove the two flat head screws from the left rear of the Cassette Housing 9 Loosen the two hex nuts holding the rear Cassette Motor Bracket 10 Remove the Cassette Drive Assembly and disconnect the Tab Sensor PCBs from the Main Harness 11 Check
37. d Valve Fitting Blue Plunger Solenoid Valve 200 uL Solenoid Valve 100uL Revision B Page 57 of 64
38. d If J 12 is disconnected from the main printed circuit board then the motor s operation can be checked while removed from the block by connecting it to an external 12 volt DC supply The motor should turn freely If it will not turn then the motor should be replaced If the motor is able to move the shutter freely then an error in the position sensing system should be suspected If errors occur only when attempting to close the shutter then check the Shutter Closed LED and sensor If errors occur only when attempting to open the shutter then check the Shutter Open LED and sensor See attached drawing showing location of the LEDs and sensors The procedure to check operation of the shutter sensors is as follows Close the LID disconnect the High Voltage and ground the TAB of transistor Q7 This will turn on the shutter LEDs and the tube present LED Disconnect J12 and apply the external power supply of 12VDC to the shutter motor to move it manually Applying the 12VDC to the blue wire pin 8 of J12 and the ground to the orange wire pin 7 of J12 will cause the shutter to move to the closed position When the shutter has moved completely to the closed position disconnect the power supply from the motor Next measure the voltages at the following points J5 pin 11 can be measured at R 137 see diagram below and J5 pin 12 can be measured at R138 These points are the anodes of the Shutter Closed and Shutter Opened LEDs used to sense shutte
39. e front panel and lift the front panel to a vertical position 3 Remove the screws securing the rear cover 4 Remove the rear cover 5 Disconnect the injector lines from the injector to be replaced 6 Remove the two screws from the bracket of the injector to be replaced Document no 8000 0006 00 Revision B Page 47 of 64 OPTOCOMP M Service Manual 7 Using a soldering iron heat the joints of the two wires on the terminals of the push pull solenoid and remove the wires from the terminals 8 Remove the injector 9 Place the new injector on the bracket and replace the two Philips head screws 10 Solder the two wires onto the terminals of the new injector 11 Reconnect the injector output lines 12 Restore power to the OPTOCOMP 13 From the MAIN MENU press the INTITALIZE key 14 Place the input lines of the injectors into a container of distilled water 15 Place an empty test tube in the counting chamber and close the lid 16 During the initialization of the injectors check for leaks from the fittings bellows etc on the injectors 17 If the injectors show no signs of leakage and are working satisfactorily replace the rear panel and top cover Document no 8000 0006 00 Revision B Page 48 of 64 OPTOCOMP M Service Manual OPTOCOMP IIB 1 Press the LID RELEASE button on the front panel and open the lid 2 Switch the OPTOCOMP off and remove the power cord from the wall socket 3 Remove the two screws fro
40. e with a tube in the well LWr Pcie Rize e E E d Pa aa a ae Ge 021357 2 7919e e i EE 8 Adjust R 148 clockwise until the sensor just turns on 9 Place a clean empty test tube in the well preferably polystyrene Adjust R 148 clockwise counting the number of turns necessary to make the sensor turn on once again or the potentiomenter has reached the end of its adjustment this is indicated by the potentiometer clicking NOTE If the sensor cannot be made to turn back on or if the number of turns to the end of adjustment is 5 turns or less the LED and or photo sensor MUST BE REPLACED After replacing an LED or photosensor it is necessary to start the adjustment process form the beginning Document no 8000 0006 00 Revision B Page 20 of 64 OPTOCOMP M Service Manual 10 Once the parameters of step 9 have been met adjust R 148 counter clockwise for half the counted number of turns This will set the adjustment approximately halfway between the detection limits of the photosensor 11 Turn the OPTOCOMP off and remove the test lead from Q 7 12 Turn the OPTOCOMP on again and program a Raw Data protocol for five sample replicates with a count time of 1 second 13 Check for proper operation of the Tube Present Sensor by placing a clean tube in the well and completely removing it after every acquisition 14 Close the lid once or t
41. emove the four screws two on each side holding the front cover of the injector housing Remove the front cover Ar TcoveR SCREWS 5 Using 11 32 wrench or nut driver remove the two hex nuts from the bracket of the injector to be replaced Document no 8000 0006 00 Revision B Page 46 of 64 OPTOCOMP M Service Manual 6 Using a soldering iron heat the joints of the two wires on the terminals of the push pull solenoid and remove the wires from the terminals 7 Remove the injector 8 Place the new injector on the studs replace the washers and hex nuts tighten with the 11 32 wrench 9 Solder the two wires onto the terminals of the new injector 10 Reconnect the injector output lines 11 Connect the injector cable to the rear of the OPTOCOMP 12 Restore power to the OPTOCOMP 13 From the MAIN MENU press the INITIALIZE key 14 Place the input lines of the injectors into a container of distilled water 15 Place an empty test tube in the counting chamber and close the lid for OPTOCOMP II place a full cassette of tubes at the first cassette position 16 During the initialization of the injectors check for leaks from the fittings bellows etc on the injectors 17 If the injectors show no signs of leakage and are working satisfactorily replace the front cover of the injector OPTOCOMP IB 1 Switch the OPTOCOMP off and remove the power cord from the wall socket 2 Remove the two screws from the top of th
42. en trying to close the shutter Press the stop key to see if the main menu will appear If the main menu is displayed then try to run a protocol Close the lid and attempt to read a tube If a shutter stall error occurs before the tube is read this indicates that the error occurs when the instrument is attempting to open the shutter If the error occurs after the tube is read but before the lid is opened this indicates that the error occurs when the instrument is attempting to close the shutter While allowing the instrument to attempt to move the shutter listen carefully to the motor Can you hear it running for a brief perhaps half second period If you can hear it running this indicates that the problem is probably with the sensor system In other words the motor is able to move the shutter but the main board may be unable to sense when the desired position is reached In this case the shutter would reach a hard stop and the motor would then draw excessive current resulting in a stall error Document no 8000 0006 00 Revision B Page 17 of 64 OPTOCOMP M Service Manual If the motor does not seem to be running at all then the problem is either with the motor mechanical jamming or the drive circuitry The shutter motor can be checked after removing it from the well chamber assembly Remove the two screws that secure the motor s mounting plate to the well chamber assembly The motor can then be removed from the block and it and its drive gear examine
43. ensor LC Lid Closed gives the status of the Lid Closed Sensor TP Tube Present gives the status of the Tube Present Sensor SS Shutter Stall tells whether a shutter stall has occurred during 50 Shutter Open gives the status of the Shutter Open SC Shutter Closed gives the status of the Shutter Closed Sensor OS Open Shutter opens the safety shutter CS Close Shutter closes the safety shutter PSO Pulse Shutter Open Opens the Safety Shutter incrementally PSC Pulse Shutter Closed closes the safety shutter incrementally Entering 1 will activate the tube present and shutter sensors QS Query Sensors With the lid Entering 2 will open the shutter Entering 3 will close the shutter Entering 4 will move the shutter slightly toward the open position Entering 5 will move the shutter slightly toward the closed position Document no 8000 0006 00 Revision B Page 14 of 64 OPTOCOMP M Service Manual TROUBLESHOOTING The following is a guide to assist in the troubleshooting and repair of the OPTOCOMP I and II Luminometer Should you still require assistance after consulting this guide please contact MGM Instruments Technical Service at 203 248 4008 or 1 800 551 1415 in the continental U S SYMPTOM Cassette Drive Error OPTOCOMP II Cassette Stall Error Display shifted High instrument backgrounds High voltage missing IL Sensor failure Low Injector Volume Keypad doesn t respond Membrane keypad cracked Memo
44. ermined using the Q A procedure Operator s Manual Section VII Maintenance With the CAL FACTOR set to 1 and the PMT FACTOR set to 1 the CALIBRATION NUMBER which is reported at the bottom of the Q A Report becomes the PMT FACTOR After running Q A from the MAIN MENU press UTILITIES 11 ENTER RESUME The OPTOCOMP will prompt you to enter a new PMT FACTOR Enter the number reported as the CALIBRATION NUMBER on the Q A report and press ENTER Rerun the Q A procedure now to insure that the CALIBRATION NUMBER is within 2 of 1 000 Document no 8000 0006 00 Revision B Page 30 of 64 OPTOCOMP M Service Manual PMT REPLACEMENT OPTOCOMP II 1 Press the LID RELEASE button on the front panel and open the lid Turn the OPTOCOMP II off and remove the plug from the wall receptacle 2 Remove the 4 large Philips head screws from the top of the PMT cover Remove the 4 smaller Philips head screws from either side of the PMT cover Remove the PMT cover 3 Stand the PMT SHUTTER BLOCK on the shutter cover Remove the two screws securing the PREAMP careful not to loose the two spacers from beneath the PREAMP SHUTTER COVER NYLON SPACER 4 Gently lift the PREAMP off the PMT It may be necessary to wiggle it slightly to free it from the pins of the PMT 5 Check the replacement PMT to make sure that the felt packing is in place about an inch from the PMT window NOTE Do not expose the PMT to any excess ambient light as this will s
45. ews removed lift the panel straight up to remove COVER SCREWS lt Document no 8000 0006 00 Revision B Page 24 of 64 OPTOCOMP M Service Manual REMOVING COVERS FROM THE OPTOCOMP IIB To remove the covers from the OPTOCOMP II B first with power applied press the Lid Release button on the front panel Open the lid and turn the instrument off and unplug the power cord from the wall socket Remove the two screws from the top of the Front Panel Lift the panel up and rotate it forward as with the OPTOCOMP IB This will allow access to the Front Panel components as well as the Front Cassette drive mechanism and associated sensors To gain access to Injectors Rear cassette drive or Power Transformer remove the six Philips head screws from the Rear Panel Lay the panel down at the rear of the instrument To remove the lid remove the three Philips head screws at the rear of the Cassette Housing Loosen the four 11 34 hexnuts located under the lid in the rear of the instrument NOTE It is not necessary to completely remove these nuts they need only be loosened since the lid is slotted illustration below shows the location of the two nuts on one side of the instrument Document no 8000 0006 00 Revision B Page 25 of 64 OPTOCOMP M Service Manual COMPONENT REPLACEMENT This section is meant for use as a guide to the replacement of certain components or modules in the OPTOCOMP I and II luminometer Should further as
46. f U31 should be directly connected to the output of the Shutter Open photo transistor and pin 13 of U31 should be directly connected to the output of the Shutter Closed photo transistor The connections to each photo transistor are as follows bm 11 57 7 peo ULL shutter Open 5v Photo Transistor RNI 16 11 ZA NEE 57 LED Ry uc J59 p Shutter Closed What to look for when shutter stall error occurs Potentiometer R96 not fully counter clockwise Connector 5 or J12 loose Shutter not moving properly because of jammed gear or loose shutter rack screw Injector precipitant dried in shutter area Problem with seating of either Shutter Open LED Shutter Open Sensor Shutter Closed LED or Shutter Closed Sensor in connectors of block assembly Short on motor Short on R79 Open on R79 Blown motor driver 08 or U9 0 If problem occurs when turning instrument on or after tube is read look at Shutter Closed circuitry first 11 If problem occurs when starting a protocol look at Shutter Open circuitry first Finally a shutter stall error may occur after hitting the LID RELEASE button while waiting at the main menu with the lid open This can happen because the CPU is constantly polling to check if the lid is open If the lid is open the CPU checks to make sure the shutter is closed If the LID RELEASE key is struck while the CPU is checking to see if the shutter is closed a shutter error may occur The LID REL
47. he MAIN PCB Remove seven Philips head screws securing the MAIN PCB Remove the MAIN PCB Check the replacement PCB for matching revision level NOTE It may be necessary to retain the EPROMS S from the original PCB for use in the replacement Install new PCB into the base and replace the seven screws Replace the wires and cables to their respective plugs as follows JI AC power from main transformer 12 High voltage cable from PREAMP J3 5vdc for PREAMP 14 Signal cable from 15 40 connector from sensors 16 Printer power J7 Printer data cable J8 Ribbon cable from display J9 Ribbon cable from keyboard 5 232 harness Injector harness 2 Motor harness Check the High Voltage setting of the replacement PCB as follows Connect a voltmeter between the J2 pins 1 and 3 on the PCB Connect a test lead Ball Clip to Alligator between U 16 pin 6 and ground This is necessary due to the fact that the high voltage is Document no 8000 0006 00 Revision B Page 32 of 64 OPTOCOMP M Service Manual not turned on unless the lid is closed and the safety shutter is open Grounding U16 pin 6 defeats the feature 10 Set the voltmeter to a range that will accept up to 1500 VDC 11 Turn the OPTOCOMP on and observe the voltmeter The high voltage setting should be 1100 VDC If necessary adjust R13 on the MAIN PCB until the voltage at 2 1100 VDC NOTE If the voltage is at or around 460 VDC and will not change whe
48. hnical service personnel Please read this manual thoroughly and familiarize yourself with the instrument before attempting to service it Covers should be removed by qualified personnel only Document no 8000 0006 00 Revision B Page 9 of 64 OPTOCOMP Service Manual SPECIFICATIONS Power requirements Sample size Weight OPTOCOMP I OPTOCOMP II Physical size OPTOCOMP I OPTOCOMP II Spectral Response Background Maximum Linear Count Rate Computer Interface Brown Out Protection Document no 8000 0006 00 100 to 240 VAC 50 60 Hz selectable 12 x 75 mm for OPTOCOMP I and II 4 X 40 mm for Approximately 25 Ib 11 3 Kg Approximately 51 Ib 23 Kg 18 w X 7T h X 15 5 d 45cm x 17cm x 39cm 18 w x 67h x 26 d 45cm x 17cm x 67cm 300 600 nm 50 RLU sec 1x10 RLU Second RS 232 Serial bi directional Automatically stores data during brown outs and power failures Revision B Page 10 of 64 OPTOCOMP M Service Manual UTILITIES The UTILITIES Menu contains parameters set by the user as well as hidden parameters which are to be utilized by service personnel The UTILITY functions that are operator accessible in the OPTOCOMP I are as follows l OPER ATOR NAME allows the user to select one of 5 names to be printed on the results header DATE TIME allows the user to adjust the internal clock calendar Q A checks detector response and instrument background BA
49. hock the PMT and cause the PMT to operate erratically for the first hour or so after power is applied Leave the rubber cover on the PMT until you are ready to install it into the SHUTTER BLOCK 6 Install the new PMT Document no 8000 0006 00 Revision B Page 31 of 64 OPTOCOMP M Service Manual 7 9 10 11 Replace the PREAMP by lining up the pins on the PMT with the sockets on the If necessary gently rotate the PREAMP to align it with the mounting holes on the ring Install the two screws which secure the PREAMP to the mounting ring using care to assure that the two nylon spacers are installed on the back side of the PREAMP Reassemble the PMT cover assembly and install it into the cassette housing Plug the instrument in and turn it on Reset the PMT FACTOR as described above MAIN PC BOARD REPLACEMENT OPTOCOMP I Tools required Philips screwdriver 1 and 2 point small flat blade screwdriver or tuning screwdriver ELSE 9 oo 9 Remove four screws holding TOP COVER two on either side and lift the TOP COVER off of the base There should be enough slack in connecting cables to allow for the TOP COVER to be placed on its right side Remove the four screws holding the FRONT PANEL two on either side and remove the FRONT PANEL NOTE There are three cables connected to the FRONT PANEL that must be disconnected before the panel can be removed Disconnect all wires and cables from t
50. ll probleTis etae peor tea ee nda 17 ADJUSTING OPTOCOMP I TUBE SENSOR eese rennen 20 COVER RBNIOVALT inea tin Quen tad RM NT 22 REMOVING COVERS FROM THE 22 REMOVING COVERS FROM THE OPTOCOMP 23 REMOVING COVERS FROM THE 25 COMPONENT REPLACEMENT iin iamini eH AUT a 26 PREAMP REPLACEMENT OPTOCOMP 1 1 01 0 0 26 PREAMP 28 PMT REPLACEMENT OPTOCOM D 28 PMT REPEACEMEBNT OPTOCONMP Il 4 osten eei ioter orae aet ene de SPERA 31 MAIN PC BOARD REPLACEMENT 32 MAIN PC BOARD REPLACEMENT 33 MAIN PC BOARD REPLACEMENT OPTOCOMP I B AND 34 REPLACING THE DISPLAY 1 36 REPLACING THE DISPLAY 36 REPLACING THE DISPLAY OPTOCOMP IB AND 38 REPLACING PUSH BUTTON ASSEMBLY 39 REPLACING PUSH BUTTON ASSEMBLY OPTOCOMP 39 REPLACING PUSH BUTTON ASSEMBLY OPTOCOMP IB AND IIB
51. m the top of the front panel and lift the front panel to a vertical position 4 Remove the screws securing the rear panel 5 Remove the rear panel 6 Remove the three Philips head screws from the base of the lid 7 Using an 11 32 socket or wrench loosen the four hix nuts holding the rear panel from the inside see diagram NOTE It is not necessary to remove these nuts they need only to be loosened the cover is slotted 8 Remove the LID ASSEMBLY Figure 4 Optocomp IIB with covers removed exposing injectors 9 Disconnect the injector lines from the injector to be replaced 10 Remove the two screws from the bracket of the injector to be replaced 11 Using a soldering iron heat the joints of the two wires on the terminals of the push pull solenoid and remove the wires from the terminals 12 Remove the injector 13 Place the new injector on the bracket and replace the two Philips head screws 14 Solder the two wires onto the terminals of the new injector 15 Reconnect the injector output lines 16 Restore power to the OPTOCOMP 17 From the MAIN MENU press the INITIALIZE key 18 Place the input lines of the injectors into a container of distilled water 19 Place a cassette full of empty test tubes in the counting chamber at cassette position 1 Document no 8000 0006 00 Revision B Page 49 of 64 OPTOCOMP M Service Manual 20 21 During the initialization of the injectors check for leaks from the fittings
52. n R13 is adjusted check the connection of the ground lead to U16 pin 6 12 Turn the OPTOCOMP off and remove the meter leads and the test lead connected to U16 13 Replace the FRONT PANEL and TOP COVER MAIN PC BOARD REPLACEMENT OPTOCOMP II 1 With power applied to the press the LID RELEASE button on the front panel and open the lid 2 Turn off power to the OPTCOMP and remove the plug from the wall socket 3 Remove the four screws from the outer shroud two screws on either side Remove the shroud 4 Remove the three Philips head screws from the top of the front panel 5 Remove the four flat head screws from the sides of the front panel and remove the front panel 6 Disconnect all wires and cables from the MAIN PCB 7 Remove seven Philips head screws securing the MAIN PCB 8 Remove the MAIN PCB 9 Check the replacement PCB for matching revision level NOTE It may be necessary to retain the EPROM S from the original PCB for use in the replacement 10 Install new PCB into the base and replace the seven screws 11 Replace the wires and cables to their respective plugs as follows JI AC power from main transformer J2 High Voltage cable from PREAMP J3 5vdc for PREAMP J4 Signal cable from PREAMP J5 40 pin connector from sensors J6 Printer power J7 Printer data cable 18 Ribbon cable from display 19 Ribbon cable from keyboard 5 232 harness J11 Injector harness J12 Motor harness
53. r bad LED requires adjustment OPTOCOMP I OPTOCOMP sensor flag binding OPTOCOMP II tube sensor binding due to reagent contamination Defective LED phototransistor or module Page 16 of 64 OPTOCOMP M Service Manual Trouble shooting shutter stall problems Shutter stall errors indicate that the main printed circuit board has detected that the shutter motor is drawing excessive current when attempting to move the shutter to either the open or closed position The first thing to check are connections at J 5 on the main printed circuit board This is the large 34 pin connector with multi colored wires which is attached to the main harness the other end of which is attached to the well chamber or block assembly Also check the connection at J 12 on the main printed circuit board a 12 pin brown connector near J 5 If the problem is not caused by loose connections then there is either a problem 1 mechanically with the shutter motor or shutter or shutter drive gear that is causing mechanical binding 2 with the shutter position sensing system see below or 3 with the drive circuitry on the main printed circuit board Also pressing the LID RELEASE button may cause a shutter stall error SHUTTER SENSOR EN One symptom that can be helpful when attempting to troubleshoot a shutter stall problem is to note when the error occurs If the error occurs when the instrument is turned on this indicates that the problem occurs wh
54. r position Both of these points should be 1 1 to 1 4 VDC measured with respect to ground If either of these points measures around 5 VDC this would mean the corresponding LED is open or the connection to the LED has opened check for continuity from the resistor to each LED if the connection is good the LED may have failed or the connection on the ground side of the LED may have come loose Log ems TT Tr S ai TEST POINTS If both of the voltages measured above check out properly check the photo transistor as follows with the shutter still in the CLOSED position and the TAB of Q7 still connected to ground locate U31 and measure the voltages at pins 11 and 13 Pin 11 should be logic low close to ground and pin 13 should be logic high above 3 5 VDC The LID must be closed and the High Voltage disconnected when the following is done otherwise the PMT may be exposed to room light Connect the external 12VDC power supply to the shutter motor again this time reversing the connection 12VDC to the orange wire pin 7 on J12 and ground the blue wire pin 8 J12 This will move the shutter to the open position Document no 8000 0006 00 Revision B Page 18 of 64 OPTOCOMP M Service Manual Once again measure the voltages at pins 11 and 13 of U31 This time pin 11 should be high and pin 13 should be low If you do not see these conditions then either one of the photo transistors has failed or a connection has come loose Pin 11 o
55. rews and shoulder washers to the other standoffs in the front panel taking care to mount the washers with the shoulder facing the front panel Connect the cables from the keyboard and display to the proper places on the MAIN PCB J9 keyboard J8 display ribbon cable J13 display backlight Replace front panel Replace top cover REPLACING THE DISPLAY OPTOCOMP 1 2 With power applied press the lid release button on the front panel Remove the four screws from the front shroud and remove the shroud 3 Remove the three screws from the top of the front panel and the four screws from the Ee pour v ee 12 13 side of the front panel Gently lift the front panel toward the front of the instrument and disconnect the lid sensor Disconnect the lid release button Disconnect the two cables from the display to the Main PCB Disconnect ribbon cable from the keyboard to the MAIN PCB Disconnect the printer harness from the main PCB Remove the front panel and place upside down on a table or bench top Remove the two screws holding the display at the end near the red and black wires Remove the two flat head screws and plastic shoulder washers from the opposite end of the display Remove the display Install the new display Document no 8000 0006 00 Revision B Page 36 of 64 OPTOCOMP Service Manual 14 Install the two pan head screws through the mounting holes in the display near the red and black wires 1
56. rom the top of the Front Panel and lift the panel to a vertical or open position Remove Upper Light Shield See OPTOCOMP II Remove Lower Light Shield Loosen the two hex nuts on the Cassette Drive Motor Bracket and rotate the bracket to provide slack in the drive belt Remove the two flat head screws from the right front of the Cassette Housing Disconnect the Tab Sensor PCB from the Main Harness Remove the Cassette Drive Mechanism Check the replacement Cassette Drive Mechanism for freedom of travel etc connect the Tab Sensor PCBs and install if satisfactory Replace the two flat head screws in the cassette housing Check the Drive Belt for proper position on the sprockets Pull the Drive Motor Bracket to remove any slack in the belt and tighten the two hex nuts on the Motor Bracket Reconnect the Front Panel at this point and restore power to the instrument From the Main Menu press Utilities 4 Enter to access the Cassette Motion Utility Press 0 to initialize the cassette drives Check to ensure that the tabs on the front drive are parked and not exposed in the cassette housing From the Main Menu press 1 ENTER to cycle cassettes and 0 ENTER for Front Cassette first to check the operation of the new cassette drive mechanism If the drive is satisfactory turn the power off and disconnect the power cord from the wall outlet once again Replace the upper and lower light shields Close the front panel and replace
57. ry Loss Document no 8000 0006 00 POSSIBLE CAUSE Broken Drivebelt Drive gear loose Drive sensor failure Drive tab bent Motor failure gear Box Debris blocking tab sensor Improper setup of cassettes Warped cassettes 82C55 U3 Photomultiplier tube Preamp Reagent residue in counting chamber Light leak due to a poor seal High voltage transformer Index locator sensor Optocomp II ONLY contaminated with reagent Bad index locator Injector lines plugged Injector fittings loose Bellows to valve connection loose Valve clogged Injector requires adjustment Bad keyboard replace Replace membrane Bad battery RAM socket Revision B Page 15 of 64 OPTOCOMP M Service Manual Instrument doesn t count Printer Failure Sensor failures Lid Sensors Shutter Sensors Tube Sensor Document no 8000 0006 00 Revision B foot interference PMT failure Pre amp failure High voltage failure 5 volt failure on main board Printer unplugged Main board power supply failure Pinter jammed with paper Printer head blown printing partial characters Loose connection on LED or phototransistor OPTOCOMP I Broken sensor module OPTOCOMP II Loose connector LED or phototransistor plugged in backward Defective LED phototransistor or module Loose connection on LED or phototransistor Sensor connected backward Defective LED phototransistor or module Shutter gear loose Shutter moto
58. self test consisting of about 20 lines of characters and 1 solid block When the self test is complete the Main Menu will be displayed If the printout is unsatisfactory for instance too light it may be necessary to adjust the print density The adjustment is located under the paper tray Remove the paper roll and remove the plug at the bottom of the tray Insert a small flat blade screwdriver or tuning screwdriver tweeker into the access hole and turn the potentiometer SLIGHTLY clockwise until the desired print density is reached CAUTION Excess print density can destroy the printhead REPLACING THE PRINTER ON THE OPTOCOMP II xp er la With power applied press the Lid Release button and open the lid Turn the instrument off and remove the power cord from the wall receptacle Remove the Front Shroud Remove the screws from the Front Panel Disconnect the wires and cables from the Front Panel to the Main PCB Remove the Front Panel and place it face down on a table or bench Remove the six hex nuts holding the Printer Assembly Document no 8000 0006 00 Revision B Page 42 of 64 OPTOCOMP M Service Manual 7 Disconnect the Paper Feed switch from the Printer Harness 8 Remove the Printer Assembly 9 Install new Printer Assembly 10 Replace hex nuts and washers 11 Connect the Paper Feed button on the Printer Harness 12 Connect the wires and cables from the Front Panel components to the Main PCB 13 Repl
59. sistance be necessary please contact Instruments Technical Assistance PREAMP REPLACEMENT OPTOCOMP I Part Number 998062 Tools required Philips screwdriver 1 and 2 point 5 16 wrench or nut driver voltmeter small flat blade screwdriver To replace the PREAMP on the OPTOCOMP I follow these steps l 2 Turn the OPTOCOMP I off and remove the power cord from the receptacle OPTOCOMP I ONLY Remove the Top Cover by removing the four screws two on either side and lifting straight up NOTE The printer harness will have enough slack to allow the top cover to be placed on its lift side during this procedure Should you prefer you can disconnect all cables which are connected to the top cover and place it aside Remove the 5 screws holding the rear panel and set it aside OPTOCOMP IB ONLY Remove the two screws securing the front panel located on the back lip of the front panel toward the rear of the instrument Pull the front panel toward you It is hinged and will stay in the upright position Remove the 6 screws holding the rear panel Lift the panel straight up and remove it from the instrument Remove the two screws from the PREAMP COVER Remove the BAR AND COVER form the PREAMP Document no 8000 0006 00 Revision B Page 26 of 64 OPTOCOMP M Service Manual 7 Disconnect the three wire harness from the PREAMP Two coaxial cables and one discreet wire harness Using a 5 16 wrench loosen and remove the nut holding the
60. the next dynode The voltage between the dynodes gives each electron sufficient energy to dislodge several additional electrons the multiplication effect At the end of the dynode string nearly one million electrons have been generated by a single photon The last electrode in the PMT The anode collects the current and conducts it to a resistor A small voltage pulse is generated and is amplified by the preamplifier PREAMPLIFIER Refer to the Preamplifier Schematic The voltage pulse is generated across resistor R2 Typically 1100 VDC is applied across the dynode resistor divider string R3 through R14 C5 is a high voltage 22pf capacitor that couples the pulse to the unity gain buffer amplifier U1 UI presents a low impedance pulse source to inverting amplifier U2 in order to maintain a high slew rate without oscillation The positive going pulse is fed through capacitor C17 to comparator U4 Potentiometer R28 sets the comparator threshold voltage Vth for approximately 25mV This value eliminates most stray noise yet is low enough to sense single photon events The output of U4 is a TTL pulse of 15 40 nanoseconds which is carried via a coaxial cable to the main PCB Document no 8000 0006 00 Revision B Page 7 of 64 OPTOCOMP Service Manual PMT SHUTTER ASSEMBLY Figure 2 is a schematic representation of the physical arrangement of the PMT safety shutter and the shutter open and closed sensors A bi directional DC motor moves the shu
61. the replacement Cassette Drive Mechanism for freedom of travel etc and install if satisfactory connect the Tab Sensor PCBs to the Main Harness 12 Replace the two flat head screws in the cassette housing 13 Check the Drive Belt for proper position on the sprockets 14 Pull the Drive Motor Bracket to remove any slack in the belt and tighten the two hex nuts on the Motor Bracket 15 Restore power to the instrument 16 From the Main Menu press Utilities 4 Enter to access the Cassette Motion Utility Document no 8000 0006 00 Revision B Page 54 of 64 OPTOCOMP M Service Manual 17 18 19 Press 0 to initialize the cassette drives Check to ensure that the tabs both drives parked and not exposed in the cassette housing From the Main Menu press 1 ENTER to cycle cassettes and 1 ENTER for Rear Cassette first to check the operation of the new cassette drive mechanism If the drive is satisfactory turn the power off and disconnect the power cord from the wall outlet once again Replace the Lid Assembly the 3 screws at the rear of the Cassette Housing and tighten the four hex nuts on the inside of the instrument 20 Replace the Rear Panel OPTOCOMP IIB FRONT CASSETTE DRIVE l e sd 11 12 13 14 15 16 17 18 19 With power applied press the Lid Release button and open the lid Tum power off and remove the power cord from the wall outlet Remove the two screws f
62. the two screws at the top of the front panel Follow the instructions above for the removal and replacement of the rear cassette drive Document no 8000 0006 00 Revision B Page 55 of 64 OPTOCOMP M Service Manual OPTOCOMP RECOMMENDED SPARE PARTS OPTOCOMP I Parts MGM Part Number Description 0110 3023 00 Well Reflector 0112 0004 00 Well Liner Assembly 0112 0005 00 Lid Seal Lower 0200 0012 00 Lid Seal Upper 0200 0005 00 Lid Spring 0330 3750 01 0112 5220 40 0112 5220 30 0112 5230 40 0112 5230 30 6110 0008 00 0112 3111 20 0112 0106 00 0112 0111 00 0111 3001 02 0111 3006 00 0200 0010 00 0200 0011 00 8000 0000 00 Parts Part Number 011 4018 05 0110 4016 00 0112 0120 00 0112 0010 00 0112 4200 00 0200 0032 00 0200 0022 00 6110 0005 00 0700 0014 01 0700 0014 02 0112 5220 10 0112 5220 20 0112 5230 10 0112 5220 20 0300 0057 00 0200 0300 00 Document no 8000 0006 00 Fuse 3 8 Amp Slo Blo Injector Line Input Red Injector Line Input Blue Injector Line Dispense Red Injector Line Dispense Blue Shutter Sensor Shutter Rack Assembly Shutter Motor Assembly Autotrol Lid Release Solenoid Lid Catch Tube Platform Tube Spring Well Liner Operator s Manual Description Cassette Lid Gasket Tube Sensor Assembly Cassette Drive Mechanism Cassette Drive Motor Assembly Lid Release Solenoid Cassette Drive Belt Shutter Sensor Tab Sensor Assembly Tab Sensor Assembly LE
63. to the instrument Document no 8000 0006 00 Revision B Page 27 of 64 OPTOCOMP Service Manual 15 16 17 18 19 and check the voltage If necessary adjust R28 until the voltmeter reads 25mV Turn the instrument off at this point Check that all connections are properly made Install the two screws to hold the PREAMP using the two nylon spacers on the back of the PREAMP Install the PREAMP cover bar and two screws Replace rear panel Replace top cover Refer to Operators Manual Section VII 5 and perform a Q A test to assure that the instrument is operating properly PREAMP REPLACEMENT OPTOCOMP II l Press the LID RELEASE button on the front panel and open the lid Turn the OPTOCOMP II off and remove the plug from the wall receptacle Remove the 4 large Philips head screws from the top of the PMT cover Remove the 4 smaller Philips head screws from either side of the PMT cover Remove the PMT cover Stand the PMT SHUTTER BLOCK on the shutter cover and remove the 3 wires Connected to the PREAMP Remove the grounding lug from the ring on the PMT Remove the two screws securing the PREAMP being careful not to loose the two spacers from beneath the PREAMP Gently lift the PREAMP off the PMT It may be necessary to wiggle it slightly to free it from the pins of the PMT Install the new PREAMP and refer to steps 10 through 15 above for installation procedures Plug the instrument in and turn it on Refer to the Operator
64. tter in front of the detection aperture whenever measurements are not being made This prevents damage to the photocathode due to exposure to ambient light with the high voltage applied Another safety measure is reduction of the high voltage when the shutter is closed When the lid is closed and a count sequence is initiated the shutter motor moves the shutter to the open position The shutter open sensor is activated when the shutter physically breaks the infra red beam of the sensor At this point the high voltage is restored the injection sequence is activated if programmed and the measurement taken Current to the Shutter Motor is monitored and a stall message is displayed and the motor is turned off if excess current is drawn See SHUTTER STALL ERROR The computer also monitors shutter travel time and the shutter motor is turned off if the shutter sensors are not activated in a preset amount of time See SHUTTER DRIVE ERROR At the end of the count sequence or whenever the LID OPEN button is pressed the shutter is moved in front of the PMT The SHUTTER CLOSED SENSOR assures that the shutter is fully closed before the lid is allowed to open TYPES OF PROTOCOLS RAW DATA Timed counts programmable from 0 02 to 650 seconds The mean value RLU and Coefficient of Variation of up to 10 replicates is reported Blank tube subtraction may be programmed using the mean value of up to 10 blank tube replicates Either injector A or B
65. w Document no 8000 0006 00 Revision B Page 12 of 64 OPTOCOMP Service Manual TWO POINT ADJUSTMENT Used with LIA software to illustrate an actual curve as well as an adjusted curve after two calibrators have been run Typically set to zero reporting only the adjusted curve DISPLAY EDITED FLAG Prints a flag to alert the user that the values printed were edited rather than actual DEFAULT MAIN MENU NAME Allows the name on the main menu default is to be customized to the users company name or any name up to 16 characters DISPLAY OVERLOAD FLAG Alerts the user to the fact that the maximum count rate has been exceeded in certain cases EXAMPLE To access and change the PMT factor on an OPTOCOMP I from the MAIN MENU press UTILITIES OPTOCOMPI SELECT START PROGRAM LIST UTILITIES INITIALIZE Press 1 1 followed by ENTER and RESUME The following will be displayed PMT FACTOR 1 06 PRESS ENTER To change the PMT factor simply enter the new PMT factor on the from panel keypad and press ENTER The instrument will automatically return to Main Menu Document no 8000 0006 00 Revision B Page 13 of 64 OPTOCOMP M Service Manual SENSOR STATUS SENSORS LO Y LC N TP Y SS N SO N SC Y FUNCTIONS 1 5 2 5 3 5 4 5 5 PSC The first line indicates the status of the sensors on the instrument the second line presents a list of test selections LO Lid Open gives the status of the Lid Open S
66. wice with no tube in the well and check that the instrument does not perform an acquisition with an empty sample chamber 15 When the tube sensor is determined to be satisfactory replace covers and panels as necessary Document no 8000 0006 00 Revision B Page 21 of 64 OPTOCOMP M Service Manual COVER REMOVAL REMOVING COVERS FROM THE OPTOCOMP I THE FOLLOWING STEPS SHOULD BE PERFORMED ONLY WITH THE INSTRUMENT TURNED OFF AND THE POWER CORD DISCONNECTED To remove the covers from the OPTOCOMP I Remove the four Philips head screws two from either side of the top cover see below Lift the Top Cover straight up and place on the left side of the instrument as shown below Document no 8000 0006 00 Revision B Page 22 of 64 OPTOCOMP Service Manual Next remove the four flat head screws two from either side of the front panel as shown below Disconnect and remove the Front Panel The inside of the OPTOCOMP shown now be completely exposed REMOVING COVERS FROM THE OPTOCOMP IB Remove the two screws from the top rear of the Front panel as shown below Lift the cover and rotate it toward the front of the instrument as shown Document no 8000 0006 00 Revision B Page 23 of 64 OPTOCOMP M Service Manual This will expose the Front Panel components Main PCB as well as the Shutter Block If access to the Preamp PMT or Injectors is necessary remove the rear cover by removing the six screws as shown With the scr
67. y gently pulling on them Replace hex nuts and washers Connect the Paper Advance Button to the Printer Harness Connect the Lid Release Button to the Main Harness Close the Front Panel and replace the two screws REPLACING KEYBOARD PCB OPTOCOMP I Tools required Philips head screwdriver 1 and 2 point IAE E 10 Turn the off and remove the power cord from the wall socket Remove the four screws from the top cover Remove the top cover and place on its side to the operator s left of the instrument Remove the four flat head screws from the sides of the front panel Disconnect the cables from the keyboard and display to the MAIN PCB Remove the front panel and place it upside down on a table or bench top Remove the six Philips head screws holding the keyboard Remove the keyboard Install replacement keyboard NOTE It may be necessary to retain the ribbon cable from the original keyboard Replace six Philips head screws Document no 8000 0006 00 Revision B Page 40 of 64 OPTOCOMP M Service Manual 11 Connect keyboard and display to PCB J9 Keyboard J8 Display Ribbon Cable J13 Display Backlight 12 Replace the front panel and screws 13 Replace the top cover REPLACING KEYBOARD PCB 1 DON OUA ee 10 11 12 15 16 17 18 With power applied press the lid release button on the front panel and open the lid Turn the

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