Fluoview FV1000 hardware manual
Contents
1. IZ PREPARATION For OPERATION Page IT 1 1 Preparation for Operation Turning the Power On 3 4 LD405 440 laser FV10 LD405 440 e Make sure that the provided shorting plug is attached to the remote interlock or that is connected to your OLYMPUS FV5 LDPSU pa equipment and the interlock is released e Setthe power switch to ON e Turn the key to the ON position e Set the shutter switch Y OPEN LD405 440 laser power supply The red lighting of the LASER EMISSION LED of NOTE hor the LD405 440 laser power supply indicates that the laser is oscillating With a certain setup the laser beam is output by simply setting the shutter switch O to ON o 3 5 LD559 laser FV10 LD559 e Verify that the remote interlock is set properly e Connect the power cord with power receptacle or O PL LASER TEMP nom ae Turn POWER switch O to ON TEMP lamp will blink in J CJ green and temperature adjustment will take place When FV10 LD559 laser power supply the temperature adjustment is completed the blinking of TEMP lamp will turn to lighting Hold on about 3 minutes until the adjustment is completed e Insert the provided key into LASER key switch and turn it ON LASER lamp will blink in red and calibration will take place When the calibration is completed the blinking of LASER lamp will turn to lighting and a laser beam will be emitted in stable output condition Hold on2. about 2 minutes until the calibration is completed When LASER key is turned ON first time after POWER switch is turned ON the calibration will be executed About calibration Calibration is performed to adjust the setting temperature of optical element inside the laser head to optimal condition H PREPARATION For OPERATION I 1 2 Page Preparation for Operation Turning the Power On NOTE NOTE Be careful as a stronger laser beam that the rated power may be emitted during calibration Temperature error would occur if LASER key switch is turned OFF during calibration In such a case turn POWER switch to OFF and start the system again In an event that a power control error PL lamp lit in orange occurred while the system is in use turn both LASER key switch and POWER switch to OFF position and start the system again H PREPARATION For OPERATION Page IL 1 3 Preparation for Operation Starting the Software 1 2 Starting the Software Turn on the microscope and power supply units before starting this NOTE software 1 Enter the user name and password to log in the Windows FV10 ASW NOTE Log in using the user name given the Administrator s authority 14 ao 2 Double click the FLUOVIEW icon on the desktop FLUOVIEW icon E TIP tif more than one user uses the FV1000 each user should personally log ini i personally For details refer to Appendix E USER REGIST
3. Manual Hardware Manual e FV1000 FV10 ASW User s Manual Quick Start Also we have prepared one service manual for this system as below Technical personnels who perform the service require to take the service training e FV1000MPE FV1000 Service Manual Reproduction copying or duplication of a part or all of this software and manual is prohibited Page 1 Registered Trademarks Registered Trademarks Microsoft Microsoft Windows Excel for Windows are registered trademarks of Microsoft Corporation Other brand names and product names are trademarks or registered trademarks of their respective owners MANUAL CONFIGURATION L SYSTEM OVERVIEW INSISTO AA L 1 1 I PREPARATION For OPERATION 1 Preparation for Operation a rrr Il 1 1 2 Replacement of CUbDES ses aan en ea asian IL 2 1 3 Centration of Mercury Burner o oo oom IL 3 1 4 Replacement of Lamps mann IL 4 1 lil TROUBLE Q amp A 1 Troubleshooting Guide r sssssssssssssss Ill 1 1 NOTATIONS IN THIS MANUAL This manual complies with the following notations lt gt Notation of Caution Notes and Tips Notation Description N It indicates caution to prevent physical damage to user or damage to product including furniture in peripheral area NOTE Indicates details of caution to prevent damage or deterioration of product
4. Mercury power suppl Unit for obtaining the transmitted cd teka y image connectedito the laser the gas laser and so on Power supply for reflected ge into a single fiber light illumination microscope through a fiber Front View I System Overview Page L 1 5 Il PREPARATION For OPERATION On This Volume This volume describes the methods for preparation for operation of the FLUOVIEW FV1000 system After completing the preparations activate the software and start observation by controlling the display on the monitor screen Please read this volume so that you can understand the system before use CONTENTS po 1 Preparation for Operation 1 1 1 1 Turning the Power ON Woman 1 1 1 2 Starting the Soflw re 2 2 ne ehe ee 1 4 1 3 Exiting from the Software 1 1 5 1 4 Turning the Power Off mmm nana 1 5 2 Replacement of Cubes 2 1 2 1 Replacing the DM Cube oom 2 1 22421 With the EV 1O ASUN F22 EE AAAA RAA AEA AAAA reeset 2 1 2 1 2 With the FV10 OPD a 2 3 2 2 Replacing the Spectral Cube 2 4 2 2 1 Removing the spectral cube 2 4 2 2 2 Fabricating a spectral cube iii oooooooooooWoWo WWW mo Waka 2 5 2 2 3 Attaching the spectral cube oom r 2 6 3 Centration of Mercury Burner 3 1 4 R
5. affect the wavelength region of fluorescence are affected by those of the spectral characteristic data of the spectral the excitation dichroic mirror used in fluorescence characteristic data of double excitation fluorescence is dropped Flare is observed The glass in use is not Use fluorescence free glass fluorescence free glass The specimen is overstained Perform optimum dyeing again or increase the offset value 12 Visual fluorescent light The light path selector in the SU is Select the visual observation light path observation is not set for the visual observation impossible light path The shutter for the mercury burner is Open the shutter for the mercury burner closed The mirror unit incorporating a Engage a mirror unit containing DM in the dichroic mirror is not present in the light path turret of the illuminator II TROUBLE Q amp A Page IT 1 3 Troubleshooting Guide Irregularity 13 The light from the laser for the ASU additional scan unit is not output Cause The properties of the combined DM cube unit for ASU do not match the irradiated laser wavelength The combined DM cube unit for ASU Remedy Engage a DM cube unit matching the laser wavelength in the light path Engage the DM cube unit in the light II TROUBLE Q amp A is not in the light path path IT 1 4 Page OLYMPUS OLYMPUS CORPORATION Shinjuku Monolith 3 1 Nishi Shin
6. from the light path selector mechanism 6 Insert the desired DM cube in the dovetail of the light path selector mechanism and tighten the clamping screw using the Allen screwdriver 7 Loosen the screw retaining the guide lock plate slide it in the direction of the arrow and tighten the screw again 8 Attach the scan unit cover to the original position TIP To use DM replaced it requires the following preparations 1 Log in FV10 ASW software with the administrative right 2 Select Tools Maintenance and bring FV1000 Setup window to appear 13 Click on System Settings button and select Filter2 tab i i4 From the drop down list located in ASU DM group box select the DM that was replaced is Click on lt Save and Close gt button and close FV1000 Setup window 6 Re boot FV10 ASW software IE PREPARATION For OPERATION I 2 2 Page Replacement of Cubes Replacing the DM Cube 2 1 2 With the FV10 OPD 1 Perform the same operations as steps 1 to 5 in section 2 1 1 With the FV10 ASU to take out the DM cube 2 Using a precision Phillips screwdriver loosen the screw clamping the DM holder plate and take out the DM and DM holder plate 0 1 01 The applicable DM Dichroic Mirror diameter is 26 x38 mm with NOTE thickness of 1 0 05mm NOTE The DM should be inserted by distinguishing the face and back Make sure that the reflective surface interference film surface
7. is expanded using a newly designed wideband head amplifier and processing circuitry High speed imaging at 8 frames per sec is made possible by fast galvano mirror In addition high speed image acquisition is possible without stopping the Z series motors used in the XYZ and XZ observations During long hours of time lapse observation a stable supply of excitation light is made possible thanks to the feedback control of the intensity of each laser Together with the photon counting function this function ensures the stability and quantitative nature of long hour observations Three fluorescence channels three lasers and AOTF are provided as standard to meet a large variety of applications With a fully motorized scan unit and motorized microscope the entire system is motorized so the scanning conditions including those of the optics can be saved and reproduced When an extension laser irradiation unit is used for photon activation aiming at causing discoloration optical simulation or uncaging of the specimen a system optimized for cell function analysis experiments can be built When the system incorporates the spectral detector unit that is composed of a 2 channel spectral detector and 1 channel filter it is possible to set the detection conditions more flexibly acquire the fluorescence spectral data and use the fluorescence isolation function L 1 2 Page System Overview Optical Path Diagram 1 3 Opt
8. mirror 26 x38 mm thickness 1 0 05mm Filter holder la ring C ESSE Barrier filter Se aa Barrier filter F Filter holder ring O DM Reflective surface interference film surface DM holder 2 Clamping screw cross head i II PREPARATION For OPERATION Page 2 5 Replacement of Cubes Replacing the Spectral Cube NOTE NOTE When replacing the DM and barrier filter take special care not to contaminate them with fingerprints etc Orientation of the filter holder ring Attach the filter holder ring by changing its orientation according to the filter thickness insert the tip of a precision flat blade screwdriver into the notch on the ring and turn the ring taking care not to scratch the filter to lock it uE y N N Filter thickness 4 6 mm Filter thickness lt 4 mm 2 2 3 Attaching the spectral cube Attach the spectral cube together with the DMs and barrier filters by reversing the removing procedure iTo use DM replaced it requires the following preparations 1 Log in FV10 ASW software with the administrative right 19 Select Tools Maintenance and bring FV1000 Setup window to appear ia Click on lt System Settings gt button and select Filter2 tab i4 Verify that Nonconfocal Detector group box is set to FV10 OPD I ls Enter information of the spectral cube that was replaced in Cube drop down ist Emi
9. of the DM comes as the face 3 Insert the desired DM and tighten the screw to clamp the DM holder plate II PREPARATION For OPERATION Page IL 2 3 Replacement of Cubes Replacing the Spectral Cube 2 2 Replacing the Spectral Cube To improve the efficiency of fluorescence detection the fluorescence waveform separating dichroic mirrors and barrier filters 2 channels of the spectral cube inside the external photo multiplier FV10 OPD can be replaced according to the excitation wavelength to be used 2 2 1 Removing the spectral cube 1 Using an Allen screwdriver loosen the two cover clamping screws provided with slip off prevention mechanisms on the left side panel of the FV10 OPD and remove the cover Q 2 Loosen the cube cover clamping screws Q inside the cover in the same way as in step 1 and remove the cube cover by holding the cover knob O IL PREPARATION For OPERATION IL 2 4 Page Replacement of Cubes Replacing the Spectral Cube 3 Loosen the spectral cube clamping screw a little using the Allen screwdriver and pull out the spectral cube by holding the spectral cube insertion knob 2 2 2 Fabricating a spectral cube A desired spectral cube can be fabricated by attaching a commercially available barrier filter and DM to the spectral cube frame Dimensional conditions for the optical components 20 1 Barrier filter 25 mm max thickness 6mm 0 1 0 14 Dichroic
10. 1A4 iun 49914 Jose 1 Z OFFISOF PN AAA son ou In Jase7 Opp sova IW0LA4 OdWFOHOOLHT N yuk Goneuiuniii Xg 104 10 99 0q BuisnoH due Aindao 1 q 4 Id3 1utod Ie9oJuo5 uoN weibeig WEJS S L p L uoljein6iyuo wajSAS P L uoneINBYUOD WSISAS MBIAJSAO Uu9 s S System Overview System Configuration 1 4 2 System Appearance and Functions The applicable microscopes are the BX61 62TRF BX61WIF and IX81F Epi Fiber Illumination Unit Laser Power Supply Microscope Mouse Top View Keyboard Power supply unit Also controls the FV1000 scan Eu RR gt unit laser combiner etc Epi Fiber Illumination Unit Illumination unit based on mercury burner connected to the microscope through a fiber 20 Flat Panel Display Monitors for displaying the laser scanning image and control panel etc Laser power supply Power supply for Multi line Argon HeNe green and LD559 lasers System Controller Used to control the FV1000 file its images etc Scan Unit Heart of laser scanning microscope composed of scanner and light detector Microscope Designed for fluorescence observations Anti vibration table EN v er er supiy Dg N Keyboard MCOMB S MCOMB D m Power supply Power supply for Multi laser combiner Control box Controls the microscope Transmitted light detector incl transmitted light illumination unit Laser combiner
11. 2 5 Reflected light laser light enters the fluorescence image 1 2 6 Fluorescence image is POOF o o oma 1 2 7 Fluorescence image is dark and noisy 1 2 8 Image is irregularly blurred or the brightness is uneven 1 3 9 Observed image is out Of FOCUS oooo oma 1 3 10 The intensity of part of the wavelength region of the spectral characteristic data of fluorescence is dropped 1 3 11 Fl re iS Observed uuu uu uuu uuu uuu aaa 1 3 12 Visual fluorescent light observation is impossible 1 3 13 The light from the laser for the ASU additional scan unit is not OEE POE u UTU il RES Ti ADE SAD SS R EP 1 4 Troubleshooting Guide 1 Troubleshooting Guide The system may be unable to manifest its full performance due to its usage as well as malfunction In case a problem occurs with the system please check the following list to find appropriate countermeasures If the problem cannot be resolved by the described remedial action please contact Olympus for repair heras 1 Laser is not output from The laser unit is not turned ON Turn on the laser unit Make sure that the the extremity of the objective 2 Fluorescence image cannot be observed emission key is set to ON The laser wavelength is not selected Check the laser wavelength to be used The manual shutter of the Open the manua
12. OLYMPUS User s Manual Hardware Manual FLUOVIEW FV1000 CONFOCAL LASER SCANNING BIOLOGICAL MICROSCOPE FV10 ASW Ver 3 0 Thank you for your purchase of Olympus microscope at this time AX8061 Retain this manual in an easily accessible place near a system for future reference CAUTION oi CAUTION FV1000 is a CLASS 3B laser product The procedures for using this system are classified as follows e Service Service means any adjustment or repair performed by highly trained and skilled technical personnels who are provided the service training following to the service manual for this system The performance has influence on the feature of this system and there is a risk which unintended CLASS 3B laser light is emitted Maintenance Maintenance means adjustment or other procedures performed by customers to maintain that this system functions properly e Operation Operation means all performance described in the user s manuals in this system CLASS 3B laser light is only emitted from the objective lens during the actual execution The User s Manuals of this system consist of the following In order to maintain the full performance of this system and ensure your safety be sure to read these user s manuals and the operating instructions for the laser unit and light source unit before use e FV1000MPE FV1000 User s Manual Laser Safety Guide e FV1000 User s Manual Safety Guide e FV1000 User s
13. RATION OF we TIP it takes 20 to 30 seconds after the FLUOVIEW icon is double clicked till the software starts up NOTE Images cannot be observed if the manual shutter of the fluorescence mirror unit is close In this case slide the shutter to the open position NOTE When you lower the objective lens please pay a careful attention so that the objective lens does not touch the specimen II PREPARATION For OPERATION T 1 4 Page Preparation for Operation Exiting from the Software 1 3 Exiting from the Software Exit from the application software and shut down Windows NOTE After exiting the application software the light of mercury burner power supply unit may exposure to specimen To avoid this perform either of the followings Close the manual shutter of the mercury burner power supply unit Turn off the mercury burner power supply unit Close the manual shutter of the fluorescence mirror unit BX61WI or 1X81 1 4 Turning the Power Off Set the power switches of the units to O OFF When using of Multi line Argon laser FV5 LAMAR 2 Turn the key to OFF position Set the power switch to OFF When using of Multi line Argon laser FV5 LAMAR Turn the key to OFF position and wait for the fan to stop automatically when the laser unit has cooled down It takes several minutes until the fan of laser stops Set the power switch to OFF Also the power supply fan will stop auto
14. eafter centering is possible with the method described in the User s Manual for the Reflected Fluorescence System that is using the burner centering knob and mirror focusing ec 5 After completing centering remove the centering target and connect the light guide When starting observation turn the collector lens focusing knob to maximize the brightness of the observation field TIP The mercury bumer does not have to be centered untl the next time tis replaced IL PREPARATION For OPERATION I 3 2 Page Replacement of Lamps The Bulb 4 Replacement of Lamps 4 1 The Bulb 1 Fully loosen the lamp housing clamping screw 1 on to of the lamp housing cover with the provided Allen screwdriver 2 Lift the lamp housing cover 2 upward to remove it 3 Turn the lamp socket 3 by 90 in the direction indicated by the arrow 4 Holding the bulb 5 with gloves or a piece of gauze depress the bulb clamping lever 4 and insert the bulb pins 6 fully into the pin holes 7 on the lamp socket Gently release the bulb clamping lever to the original position to secure the bulb To prevent reduced bulb life or cracking do not touch the bulb with bare hands If fingerprints are accidentally left on the bulb wipe the bulb with a soft cloth 5 Side the lamp housing cover onto the housing base from the above Tighten the clamping screw 1 while pressing downward on the cover Caution for Bulb Re
15. eplacement of Lamps 4 1 4 1 The Bulb sds bsa me an an ina Man leise 4 1 4 2 The Mercury BUENO cio 4 2 Preparation for Operation Turning the Power On 1 Preparation for Operation 1 1 Turning the Power On Set the power switches of the following units to I ON e Power Supply Unit FV10 PSU FV5 LAMAR 2 power supply e Mercury Burner Power Supply Unit e Microscope Control Box BX UCB or IX2 UCB 2 Set the power switches of the PC and monitor to I ON 3 Turn on the lasers as follows 3 1 Multi line Argon laser FV5 LAMAR 2 e Set the power switch to ON This starts the fan of the laser e Turn the key to the ON position 3 2 Multi line Argon laser FV5 LAMAR e Set the power switch to ON This starts the fan of the laser e Turn the key to the ON position FV5 LAHEG 2 power supply It takes a few tens of seconds after the key is set to ON till the A eye A laser oscillation begins O POWER ON Cesar 3 3 Helium Neon Green Laser FV5 LAHEG 2 or FV5 LAHEG e Turn the key to the I ON position It takes a few tens of seconds after the key is set to ON till the laser oscillation begins FV5 LAHEG power supply To ensure stable laser light output it is recommended to NOTE warm up the laser power supply after turning it on The warm up period should be 10 minutes or more when using the Argon laser power supply and 30 minutes or more when using the Helium Neon Green or Red laser power supply
16. gently with gauze slightly moistened with absolute alcohol IT PREPARATION For OPERATION IL 4 2 Page Replacement of Lamps The Mercury Burner 5 Attach the socket section with burner to the original position and tighten the clamping screw 1 NOTE Align the external edges of the lamp housing with those on the socket section and push the lamp housing straight downward Burner Service Life USH 1030L 300 hours TIP This value assumes light cycles composed of 2 hours of lighting and 30 minutes of l extinction Do not turn it on and off at a shorter cycle than the above for this will shorten the service life of the burner Ternama anna After replacing the burner reset the hour counter to 0 0 as outlined above H PREPARATION For OPERATION Page I 4 3 HI TROUBLE Q amp A On This Volume This volume describes how to deal with troubles with the FLUOVIEW FV1000 system If any irregularity is observed read this volume before calling for service If the irregularity cannot be resolved by the described remedial action please contact Olympus for repair CONTENTS ss 1 Troubleshooting Guide 1 1 1 Laser is not output from the extremity of the objective 1 1 2 Fluorescence image cannot be observed 1 1 3 Transmitted image cannot be observed 1 2 4 Image is disturbed aman rare 1
17. ical Path Diagram Barrier filter Xx DM Ad 4 Barrier filter Laser beam Grating 3 Pinhole Galvano mirro Grating Slit h2 IR ss UV ka A gt he 1 h1 Slit vis u aa e en a u k o NM gt os al Mercury light Supply I System Overview rc Page L 1 3 obed P L I A IAJ9AO WEIS S I pun uogeuluinI XI 403 10399390 gt 19q14 Ida e9ojuo9 uoN SI30LA3 OXI 0LAd edossoJ IN BuisnoH duue1 Aindaowy M y 10 xog oU05 OdWOHOOLHT N Keldsiq joued 3214 02 TON TE Ie en a XI 1S 01A4 2d0ISOJIIN EA XI 10 yun ULIS A AOUN pug pieog a0ej19 uU Dd BI9d 0 LAS BIEMYOS MINIY padbueapy SS ASY OLAJ MS LAI 1359 0143 9JEMPYOS PODUBAPY Y Y 19101340 weJsAg NSd 01A4 Jun Ajddng samog ASY 0LA3 yu ue5s euonippy OdO0l A4 NS 104 10 99 9q EI0JUOJ UON ESSOTO LA loan dao das OLAS in AS HOIBNLISAS OLAS a ELFOTO LAS yu S5s ion 4j4 S3H V SA4J Z OF ROTA SOPQI SAd PL uoneigia HUY Ada OLAS HS IMXS 10 RosH v 9n Josuas JUIISIJON H yun ueos Of DENE ouueyo yyy euonippy Xg 10 Pe N EOS adosso1s W IMAS D LAS ONSA adbaidasoN uonisod lBu vans 0LAS ATXNS O LAS N JISOd 3 s NIX 335 1174 999id soN Buibuims zuvn vrsndl O rs pas 1269 To BENJ 0 LAI S19 88p 86b SIBWOIW O LAS ANana 0 LA J9UIQUIOS Jose AQ2304 0
18. juku 2 chome Shinjuku ku Tokyo Japan OLYMPUS EUROPA HOLDING GMBH Wendenstrasse 14 18 20097 Hamburg Germany OLYMPUS AMERICA INC 3500 Corporate Parkway Center Valley Pennsylvania 18034 0610 U S A OLYMPUS SINGAPORE PTE LTD 491B River Valley Road 12 01 04 Valley Point Office Tower Singapore 248373 OLYMPUS AUSTRALIA PTY LTD 31 Gilby Road Mount Waverley VIC 3149 Melbourne Australia OLYMPUS LATIN AMERICA INC 5301 Blue Lagoon Drive Suite 290 Miami FL 33126 U S A Printed in Japan 201012 01
19. l shutter fluorescence mirror unit is closed Manual system only The reflective mirror inside the Engage the reflective mirror in the light fluorescence mirror unit is not in the path light path Manual system only The objective is not in the light path Engage the desired objective in the light path When using a manual revolving nosepiece be sure to stop the objective in the click position The laser beam is too weak Increase the laser intensity The properties of the combined cube Engage a DM cube unit matching the unit used for the ASU auxiliary scan selected laser wavelength in the light unit or OPD non confocal point detector do not match the selected laser wavelength The confocal pinhole diameter is too Increase the pinhole diameter small The excitation Dichroic Mirror Engage a DM optimum for the observed selection does not match the fluorescence and excitation laser observed fluorescence wavelength wavelengths and excitation laser wavelength The spectral dichroic mirror and Engage a spectral DM and barrier filter barrier filter selections do not match matching the observed fluorescence in the observed fluorescence the light path wavelength The acquisition wavelength region Set an acquisition wavelength region setting is not suitable for the matching the observed fluorescence observed fluorescence wavelength Spectral detection system only The fluorescent dyeing method and Select a lase
20. matically For details refer to the instruction manual of laser units In case of LD559 laser Turn LASER key switch to OFF position and then turn POWER switch to O OFF position II PREPARATION For OPERATION Page I 1 5 Replacement of Cubes Replacing the DM Cube 2 Replacement of Cubes 2 1 Replacing the DM Cube Cd 2 1 Replacing the DM Cube The DM cube is used to connect the light path of the optional FV10 ASU Auxiliary Scan Unit or FV10 OPD Non confocal Point Detector with that of the scan unit and should be selected according to the observation method 2 1 1 With the FV10 ASU Set the light path of the scan unit to the LSM light path This can be done with the FLUOVIEW software For details refer to the Users Manual for the FLUOVIEW software 2 Loosen the four cover clamping knobs on the lower part of the right side panel of the scan unit and remove the cover Q 3 Using an Allen screwdriver loosen the screw retaining the guide lock plate move the guide lock plate in the direction of the arrow engage it with the pin below the guide and tighten the screw again to lock the guide Before moving After moving II PREPARATION For OPERATION Page IL 2 1 Replacement of Cubes Replacing the DM Cube 4 Using the Allen screwdriver loosen the clamping screw retaining the DM cube 5 Pull out the DM cube insertion knob toward you and take out the DM cube
21. n image monitor As shown in this figure the confocal optics incorporates a confocal aperture on the optically conjugate position Light detector confocal plane with the focus position Confocal aperture to eliminate light from other part than the focus position This causes the extraneous light to be viewed as darkness in the observation image it is possible to slice optically a tissue specimen that has thickness On the other hand an ordinary optical microscope the light from other part than the focus position is overlapped Laser with the imaging light of the focus Objective NO Specimen position so the image is blurred in overall The laser beam that has transmitted through the specimen is detected by the transmitted light detector and provides the transmitted image which is not a Light detector confocal image However when the fluorescence images of the transmitted and confocal images are combined it is possible to obtain very important information on the specimen I System Overview Page I 1 1 System Overview Features of the FV1000 1 2 Features of the FV1000 I System Overview 1 The photon counting mode is newly provided to improve the sensitivity and S N and to enable quantitative optical intensity measurement Photon counting makes possible long hours of quantitative observation by completely eliminating analog derived drift The dynamic range in which photon counting is possible
22. placement During Use or Right After Use The bulb and the lamp socket are areas near these will be extremely hot during and right after use Set the main switch to O OFF disconnect the power cord from the wall outlet then allow the old bulb and lamp housing to cool before replacing the bulb with a new of the designated type II PREPARATION For OPERATION Page IL 4 1 Replacement of Lamps The Mercury Burner 4 2 The Mercury Burner 1 Loosen the socket clamping screw 1 using the Allen screwdriver 2 Hold the upper section of lamp housing and pull it upward to remove the socket section NOTE To prevent malfunctions do not hold the lamp housing by the centering knobs 2 3 Place the socket section upside down as shown in Fig 5 TIP The lamp housing is equipped with the holder for i transportation in the factory shipment condition or with an old burner when the burner is replaced Remove the holder A A A A 4 Attach the positive pole of a specified mercury burner to the fixed mount on the upper side and the negative pole to the mount on the lower side NOTE Be sure to use the USH 1030L mfd by OLYMPUS or HBO103W 2 mfd by OSRAM burner Be careful and avoid leaving fingerprints or contaminants on the mercury burner Otherwise there is a danger of explosion due to distortion of glass caused by the stains If the burner is contaminated clean it by wiping
23. r optimum for the fluorescent excitation wavelength do not match dyeing method Focus is not adjusted Adjust the focus The PMT voltage of the detector is Increase the PMT voltage too low HI TROUBLE Q amp A Page IT 1 1 Troubleshooting Guide s Irregularit 2 Fluorescence image cannot be observed Cause The offset value is too large Remed Decrease the offset value The detector for the channel to be detected is not selected Select the detector 3 Transmitted image cannot be observed The transmitted light detection channel is not selected Select the transmitted light detection channel The transmitted light filter for the microscope is in the light path Disengage the filter from the light path The PMT voltage of the transmitted light detection channel is too low Increase the PMT voltage The offset value for the transmitted light detection channel is too large Decrease the offset value Image is disturbed Reflected light laser light enters the fluorescence image Fluorescence image is poor Fluorescence image is dark and noisy II TROUBLE Q amp A The system installation location is subject to excessive vibrations Extraneous light such as the light of a fluorescent lamp is detected The barrier filter is set erroneously or absent The set acquisition wavelength is overlapped with or too close to the e
24. rescence image is The excitation Dichroic Mirror Engage a DM optimum for the observed dark and noisy selection does not match the fluorescence and excitation laser observed fluorescence wavelength wavelengths and excitation laser wavelength The spectral dichroic mirror and Engage a spectral DM and barrier filter barrier filter selections do not match matching the observed fluorescence in the observed fluorescence the light path wavelength The acquisition wavelength region Set an acquisition wavelength region setting is not suitable for the matching the observed fluorescence observed fluorescence wavelength Spectral detection system only The confocal pinhole diameter is too Increase the pinhole diameter small The scanning rate is too high Decrease the scanning rate The HV setting is too high Decrease the HV and increase the gain An alternative remedy is to decrease the scanning rate and decrease the HV The width of the acquisition Increase the width of the acquisition wavelength region is too small wavelength region Dyeing is too pale Perform optimum fluorescent dyeing 8 Image is irregularly The specimen or stage is tilted Install the specimen and stage properly blurred or the brightness is uneven 9 Observed image is out of The focus is adjusted improperly Adjust the focus in visual observation focus 10 The intensity of part of The spectral characteristics of the Use an excitation DM that does not
25. s TIP a Hint or one point advice appears with TIP E attached SYSTEM OVERVIEW On This Volume This volume describes the overview of the FLUOVIEW FV1000 system Please read this volume so that you can understand the system before use CONTENTS ii 1 System Overview 1 1 1 1 PAN GIS cei 1 1 1 2 Features of the FV1000 oo m m J Ju 1 2 1 3 Optical Path Diagram aan akang 1 3 1 4 System Configuration 2 2 32 2 1 4 1 4 1 System Diagram u nr rn ana ana ATA TATATATA 1 4 1 4 2 System Appearance and Funco tions rrenan 1 5 System Overview Principles System Overview OLYMPUS FV1000 is a confocal laser scanning biological microscope system featuring improved basic performances sensor system scanning system and illumination system performances by considering the live cell observations with which long hours of stable measurement of weak fluorescence is required This microscope is equipped with 3 fluorescence channels 3 lasers and AOTF to meet various applications in a wide range of advanced research fields 1 1 Principles A laser scanning microscope converges the laser beam into a small spot using an objective and scans the specimen in the X Y direction using the laser beam The microscope then captures the fluorescent light and reflected light from the specimen using light detectors and outputs the specimen image on a
26. ssion DM text box BF CH1 text box and BF CH2 text box respectively i iG Click on lt Save and Close gt button and close FV1000 Setup window 7 Re boot FV10 ASW software IT PREPARATION For OPERATION IT 2 6 Page Centration of Mercury Burner 3 Centration of Mercury Burner For the reflected light fluorescence observation refer to the User s Manual for the Reflected Light Fluorescence System Since this system introduces the light of a mercury burner through the light guide the burner centering method is slightly different from that described in the User s Manual for the Reflected Light Fluorescence System This section is intended to describe the method specific to this system 1 Turn the shutter O fully toward the bottom to block the light When the light guide is disconnected another built in shutter is engaged automatically in the light path to ensure safety 2 Remove the light guide from the ULH holder and replace with the centering target O 3 Turn the shutter O toward the open direction The arc image of the mercury burner will be visible on the screen O of the centering target O Keep the shutter O closed except for centering operation to prevent the centering target from being heated up IL PREPARATION For OPERATION Page I 3 1 Centration of Mercury Burner 4 Turn the collector lens focusing knob on the lamp housing to bring the arc image into focus TIP E i Her
27. xcitation laser wavelength Spectral detection system only The barrier filter that can cut the wavelength of the laser light irradiated from the ASU auxiliary scan unit is not selected In the case of a spectral detection system the acquisition wavelength setting may be inappropriate The front lens of the objective is dirty When an objective with correction collar is in use the correction collar is adjusted improperly The cover glass thickness is inappropriate The laser beam is too weak The fluorescent dyeing method and excitation wavelength do not match each other Contact Olympus Turn the room light low before acquiring image Engage a barrier filter that can cut the excitation laser wavelength in the light path Select an acquisition wavelength that is not interfered with by the laser wavelength Note that when the confocal pinhole is large and the BS20 80 excitation DM is used penetration of laser light may become large Engage a barrier filter that can cut the laser wavelength from the ASU in the light path With a spectral detection system change the acquisition wavelength setting Clean the objective front lens by wiping it with a piece of gauze Adjust the correction collar properly Use a cover glass with thickness of 0 17 mm Increase the laser intensity Select a laser optimum for the fluorescent dyeing method IT 1 2 Page Troubleshooting Guide po 7 Fluo
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