Jenway 6300 Spectrophotometer Service Manual
Contents
1. 19 ABBE EA 30 279 922 434 ONG Hdd NAG 95797712 31 VG 0 IS 9853 98541 2 82 84 70385237 1 988751 2 41 IND 227 VIS AVMSID 202 9 GL 123007 1 220558 1 0 5319 SH3LAWVIG XSY 1 NI 4 JVIXILYN SYD 30 asnu pue 14 4 3141 SNWIG TIY 31725 HHHH TWHINID 931 1 5 5531 4 4 00 910024 951 1 10 LOH JON GPT 09 390 610 580 610 pTI 009 60d 7 500 A 50 810 087 090 065 9154 250 00 272 110 10095 950 950 200 60D _ 550 310 44 101 200 104 1801093155 720500 0 105 vee ON 90D pou isse 600 27 800 127 100 509 060 200 109 1092. an 780 esn 40 UTE 8141 3 4 8 38 lt lt lt lt 3383 HE 1 19149 OVANA 144444 Lerere 8 133 R He 24341913 eran 83459404 Ett PEELED ERER BO a av gt nv 32372021 B 28854 8 8 7 390539 553400 0 1 1404 900H LYM N ong suo BR 8 5588 81618 5 4 Ja 43 HiHi 1 4 JILVWSHIS 104100 Bass hE ABT ABTH 170 534 8169 QW 553 011 osn 40 9 84 22 _ I 31111 09929 940150949 psrw
3. 5141 31925 SSTINN 32 94370 MUST UPPERMOST 17 BLUCK MUST ASSEMBLED THIS WAY UP 6 0MM NOTE NOTE SIDE MARKED WITH 2 sure a Bos 069 SATONV sanoa HSINI4 522 0 5 9 NI 1270 9 asn 1 140 4 400 s 5141 31925 SSTINN 01 2385 0059 ZAW E 29140 JALIL 45 4 Od 02 N LJ _ L n 2 A J 284 E 72 S u a u gt S SOLENOID SHUTTER n p Ur Bk 7 02 0 9 PINS 5 8 PL2 amp SECURE TO CABLE Ur 7 0 2 4 WIRES FROM STEPPER MOTOR WIRE FORM WITH TYWRAPS ITEM 3 10 Br 7 0 2 NOTE FIT GRATING AT FINAL OPTO SENSOR VIEWED FROM REAR MIA 7 02 ASSEMBLY LEVEL 16 0 2 WIRING DIAGRAM _
4. TIY 3125 28 suet 82 559 9535 api hom gz 184 1495 2 gt pe 82455 Wet LED exs EesaS Ha 1303 22054 52 S 852 fom 219548 nom 719525 fam ae 89225 99255 2754 2345 66525 g hom WaT hom gz 255 592525 az 5 59 z Aa and api hom ae 522 555 82 81 51 lt 3325 0938 lt x 5 5 8155 229 thom 45 15 omg 5 wang fom 15 ONF 6 275 8 16575 9575 as Iwy m 8384 250 INIS zta 25 hom 5 675 5 5 56635 55 5 9 6125 9545 ue 83 6505 5 renas 8 5 5 20735 291758
5. 6300 Spectrophotometer Service Manual Main Index Section 1 Section 2 Section 3 Section 4 Section 5 Section 6 Section 7 Section 8 Section 9 Section 10 Section 11 Jenway 6300 Ser Man Introduction Quick Reference System Description Optical Description Electronic Description Software and Operation Diagnostics Maintenance Circuit Diagrams Assembly Diagrams Spare Parts List Section 1 Introduction 1 0 Index to Sections 1 1 About This Manual 1 2 Using This Manual 13 Warnings amp Safe Practice 1 4 Standards amp Certification 1 5 Ordering Spares 1 6 Returning Items 1 7 Contacting Jenway Limited Jenway 6300 Ser Man Introduction 1 0 Index to Sections Section 1 1 0 1 1 12 1 3 1 4 1 5 1 6 1 7 2 2 0 2 1 2 2 2 3 2 4 2 5 2 6 2 7 3 3 1 3 2 3 3 3 4 4 41 42 43 44 Introduction Index to Sections About This Manual Using This Manual Warnings amp Safe Practice Standards amp Certification Ordering Spares Returning Items Contacting Jenway Limited Quick Reference About Quick Reference Specification Main Sub Assemblies Power Supply Voltages Signal Levels Error Codes Special Key Functions Test Solutions System Description Background Sub Assemblies Accessories Outputs Optical Description Light source Grating Shutter and Filter Signal Detect
6. 2 4 8 3313 010 4041934 0089 UET 1AL AJEWISSY 13894 IUL susug gt Sa TONY 22 370H 4 9 SUL JN 0153713 1077 13 05 SNIVW 9 HILIMS SNIVW 11 01 11 02 11 03 11 04 11 05 11 06 11 07 11 08 Section 11 Spare Parts Packed Instrument Top Case Assembly Microprocessor PCB Lower Case Assembly Monochromator Assembly Detector PCB Power Supply PCB Rear Panel Assembly Jenway 6300 Ser Man 52 Part Number 6300 SPARE PARTS LIST Description Section 11 01 033 227 060 084 630 026 013 046 013 083 013 123 013 181 TBA 630 028 Section 11 02 630 025 630 032 Section 11 03 630 013 630 512 Section 11 04 010 040 060 040 060 311 Section 11 05 009 063 012 075 032 005 630 516 012 089 Section 11 07 630 506 002 054 012 085 020 002 020 006 Section 11 08 630 504 002 070 Jenway 6300 Ser Man 630 501 Packed Instrument Packing case complete with inserts Disposable cuvettes 4ml pack of 100 Instruction manual Mains cable without plug Mains Cable U S A plug Mains Cable European plug Mains Cable U K plug Service Manual Dust Cover 630 010 Top Case Assembly Membrane keypad Sampl
7. ON 900 37 09 pouSisst 505 WI 214 100 t09 71081 96 100 zoo 28022 201 200 09 poudisse 105 8 NOIS 509 100 009 7001 335 100 100 WL 085 100 009 604 075 100 303 49019 160 200 908 WTI 900 E0 sod JON 09 100 808 601 200 B 1 5 LAr 9 1 704 JON 7 5 paniis ei 20 010 oog 00 100 104 5812 985 01 560 200 104 peudisss 8 PouBiset 004 650 610 00 mar 8 15177 72 VP fra ayes ones 4 pue pang 7 syweuodwoo wy msw 01 994 pedsuy umoys se 14 6041504 10429010 1 014 8 UMOYS 10974 314 5 2851 8 01 1 60
8. 193559 01 19 1 0 1 9599 LN3WH YLLY 02 W3LD C3QIS 43 709 30 35993 SCISNI OL HOVLLV 23414 13 345 0059 9760 8 11610 291028 12610 9 9 xass 3 mowun a AJEWISSY 3592 IUIL poy NOILISCF Odd 861 9812 10 ar SATONV Sys Avil sena wrar ije e 7943 39 UO SS W4ad asn 5 COPPER SIDE 2 MAINS ON OFF SWITCH FROM TRANSFOMER LEADS 110MM LUNG RED LEAD TO PIN 1 RED GREY VIOLET ORANGE TRANSFORMER WIRING DETAIL VIEW ON REAR OF CONNECTOR 31 13385 0069 xassa 9 5 5 3592 dol 5 4 SATONV seal ad HSINI4 542 0 5 ug ssjwuad WW 9 asn aq snw
9. Section 8 Maintenance 8 1 Routine Maintenance 8 2 Dismantling 8 3 Optical Alignment 8 4 Energy Levels 6 5 Wavelength Calibration 8 6 A to D Calibration 8 7 Dto A Calibration 8 8 Performance Verification Jenway 6300 Ser Man 34 8 1 Routine Maintenance The Jenway Limited Model 6300 Spectrophotometer has been designed to give optimum performance with minimal maintenance It is only essential to keep the external surfaces clean and free from dust and to ensure that the area around and underneath the unit is also clean and dust free The sample area should be kept clean and accidental spillage should be wiped away immediately as some corrosive or solvent based samples or standards may attack the materials used in the sample chamber and cell holders To give added protection when not in use the unit should be disconnected from the mains supply and covered with the optional dust cover 630 028 For longer term storage it is recommended that the unit be returned to the original carton for re shipment a further external packing case suitable for the method of carriage should be used Details of all routine maintenance tasks including changing the lamp can be found in Section 4 of the Instruction Manual 8 2 Dismantling Before dismantling any of the following sub assemblies ensure that the unit is switched off and the power cable is disconnected from the supply Do not attempt to dismantle these units unless they
10. 1 30115215 8 1 221028 ILETO 3191 211 3 25 094908 YOLISLICOWABES 815 842 31111 59849 8 5 905 089 7434 580 O SW CALVO WO dev E 0171 558558 NIVI 55 238 anoa 0 204 55 97 5 16 0811 221058 1150 194 zaw 211 uoyssiwed Sin jo 9 10 we mn GaLVLS 5 1 you 8 511 SNIA 1 25 6531 53 101 21235 pepiaosd 99 4 5 atlas son cof 8 SYD ION H 60 950 700 PausIssy ION 600 L09 590 610 0129 900 650 500 909 2 8155 ION SOC zod 509 90 00 49001 660 200 09 poudissy OLE 70 100 09 pougissy 1
11. ques 34525 poet Eas eer 37127143034 223 fom az 355 Tue 200188 54745 3 7 anez 5 59 312 143238 143 fom 7 555 Tengs TOTAS 35527 8 1934 3 3 5 gt 14 5 21901 6 ter s 16 091 ADDES 211028 1460 121 816 9W9 9553 I gt A 25 ZAW COD cee 40155112929 Ni Sin QaLViS ASIMYAHLO Z1vos jo SN 10 peonposdas you pue 9 141 5531 5 101 31 25 LON 1 1 SYD NOILNWD a b0 00 605 8
12. In correct operation when this 200mV level is detected the grating rotates in the same direction for a further 200nm but in 0 5nm steps For each step an increasing output is returned until the peak is reached The grating is stopped when a decrease in output is measured and by reversing again for one step the position of the peak is verified If the grating rotates the full 200nm without the output increasing and then falling the error code Err 4 no zero order peak found is returned This test will be failed if samples or cuvettes etc are left in the sample compartment during the start up routine if the sample holder or sampling device in the sample chamber is incorrectly fitted aligned such that it obscures the light beam Also if the incorrect lamp is fitted if the lamp has not been fitted correctly if the dark shutter is stuck in the closed position or through contamination degradation or misalignment of other optical components 6 2 Photometrics When the Start Up test has been successfully completed the display returns to the last settings of wavelength and mode used before the instrument was turned off The wavelength displayed on the lower digital display can be adjusted by using the up and down arrow keys to select the value suitable for a specific application The cursor can be moved along the menu bar at the bottom of the screen by using the right and left arrow keys Moving the cursor under the T icon will enable the T
13. Key Functions 2 7 Test Solutions Jenway 6300 Ser Man 2 0 About Quick Reference This section contains a selection of the key information that is often forgotten or difficult to find when required Use Quick Reference as a memory jogger but for more information check out the references to the main sections on each point 2 1 Specification Wavelength Range Wavelength Resolution Wavelength Accuracy Spectral Bandwidth Light Source Optics Transmittance Absorbance Concentration Factor Photometric Accuracy Photometric Noise Stray Light Photometric Stability Readout Outputs Supply Voltages Power Dimensions Weight Jenway 6300 Ser Man Also see Section 1 2 of the Instruction Manual 320nm to 1000nm lnm 2nm 8 0nm Tungsten Halogen Single Beam Range 0 to 199 9 Resolution 0 1 Range 0 300A to 1 999A Resolution 0 001A Range 300 to 1999 Resolution 0 1 1 Units ppm mel blank 0 to 999 9 1000 to 9999 1 Less than 1 Less than 0 5 T Better than 1 per Hour after warm up Custom LCD Analogue 0 to 1999mV RS232 Serial 115 230 V a c Less than 50W 365 w x 272 d x 160 h mm 6Kg 10 2 2 Sub Assemblies Also see Section 11 Spare Parts 630 010 630 510 630 508 630 012 Jenway 6300 Ser Man Top Case Assembly includes the following 630 025 Keypad 630 013 Display PCB Lower case Assembly includes the
14. and request help or clarification before proceeding Carrying out this procedure will invalidate the manufacturers optical performance specification and should only be Jenway 6300 Ser Man 37 undertaken by personnel trained and equipped to verify optical performance of the instrument Instrument covers should only be removed by engineers trained in safe working practices and aware of electric shock hazards Removal of the monochromater cover will invalidate any warranty claim regarding performance to specification Do not look directly at the light source use eye protection or the lamp dimming function when necessary Do not touch any optical surfaces If in any doubt DO NOT PROCEED Ensure or verify by replacement that GENUINE JENWAY LAMP is fitted refer to the instruction manual for details There are many lamps that look similar but the filament position is critical and can only be guaranteed on Jenway lamps replacing the lamp may correct the problem without any further re alignment 1 Remove top half of the case by undoing four recessed screws in the corners of the base 2 Place top half of case behind the base taking to trap or strain the connecting cables 3 Remove the two screws retaining the black monochromator cover in the bottom half of the case Remove the cover and place to one side DO NOT TO TOUCH ANY OPTICAL COMPONENTS 4 Taking all precautions to avoid the risk of e
15. are in a clean dry and dust free environment Use a soft lint free cover on any benches that will have casework displays or keypads placed on them Use approved and tested anti static procedures when dismantling any electronic sub assembly or PCB and store these items in anti static containers where necessary General Access to all major sub assemblies can easily be gained by removing the top half of the case Access to the lamp housing can be made through the lamp access panel on the rear of the unit Jenway 6300 Ser Man 35 The sampling accessory be accessed through the sample chamber lid Top Bottom Case Assemblies The top and bottom case assemblies can easily be separated by unscrewing the four recessed screws in each corner of the base This should be done without inverting the unit by moving it forward over the front edge of the bench to unscrew the front two screws and then turning it around to do the same with the back two Turn it back round and then the top half of the case can be lifted off the bottom half take care not to strain any cables between the top and bottom sections Should it be necessary to work on the top case assembly by itself it is simply a matter of disconnecting the plug from SK5 on the power supply PCB then the top can be completely removed Microprocessor Display PCB The microprocessor display PCB is mounted in the top case assembly To remove it disconnect SK1 on the ribbon cable to
16. blocked by a shutter switched in and out by an electronically operated solenoid Before passing into the sample chamber the monochromatic light passes through an infra red filter when wavelengths below 360nm are selected Similar to the shutter this filter is switched in or out by an electronically operated solenoid under microprocessor control Signal Detector An 51133 photo diode is used as the detector on the 6300 it is mounted directly onto the detector PCB behind the lens block The detector PCB carries out all of the analogue signal processing including the A to D conversion and has its individual calibration data stored in an on board For more information see Section 5 2 Detector Circuit Jenway 6300 Ser Man 21 Section 5 Electronic Description 5 1 Power Supplies 5 2 Detector Circuit 5 3 Microprocessor and Display Jenway 6300 Ser Man 22 5 1 Power Supplies See Section 9 1 Power Supply Schematic The ac mains supply is reduced by the torroidal transformer giving two low voltage outputs from the secondary windings First the 20 ac output from the transformer is rectified by BR1 the 30V dc output from this rectifier is used as the input to two L4960 switch mode regulators and REG 3 Tungsten Lamp Supply The output of the switch mode regulator REG 3 is set to 12V dc by VR1 The 12V output can be reduced to 5 1V by a logic 1 on the gate of TR2 This happens during the sta
17. can be checked and carried out as follows Remove the top half of the case by undoing the four recessed screws in the corners of the base Place the top half of the case behind the base taking care not to trap or strain the connecting cables Taking all precautions to avoid the risk of electric shock connect the power cable and depress the right arrow key gt while turning the power switch on The display should show a mV reading on the top row followed by the wavelength on the 2 row and T at the bottom left hand side Connect the positive lead of a voltmeter to the anode of D4 on the power supply PCB and the negative lead to the black analogue output socket on the rear panel The anode of D4 is the contact close to SK3 that is also connected to the plate through hole between D4 and SK3 Select a range suitable for measuring up to 5V dc Press the down arrow key to reduce the wavelength to 30nm the voltmeter should read approximately 0 100V Press the down arrow key again to reduce the wavelength to 40nm the voltmeter should still read approximately 0 100V Press the down arrow key again to reduce the wavelength to 50 the voltmeter should now read approximately 5 00V Press the up arrow key and return the wavelength setting to 40nm and the voltmeter reading should return to approximately 0 100V Jenway 6300 Ser Man 40 Should these results not be obtained function of opto coupler should be checked
18. path See Section 7 2 2 7 Test Solutions 1 Holmium Perchlorate 5 w v solution of Holmium Oxide in 1 4N Perchloric acid this will give absorbance maxima at 361 4 416 1 451 1 485 3 536 5 and 640 5nm Potassium Dichromate 100 0mg I in 0 005M Sulphuric Acid use the Sulphuric Acid as the blank This will give an Absorbance value of 1 071 at 350nm Potassium Dichromate 50 0mg I in 0 005M Sulphuric Acid use the Sulphuric Acid as the blank This will give an Absorbance value of 0 536 at 350nm Sodium Nitrate 505 in deionised water should give less than 0 1 Transmittance at 340nm All these solutions are hazardous and the manufacturer suppliers safety precautions should be carefully followed at all times in preparation use and storage Jenway 6300 Ser Man 16 Section 3 System Description 3 1 Background 3 2 Sub Assemblies 3 3 Accessories 3 4 Outputs Jenway 6300 Ser Man 17 3 1 Background The model 6300 is a single beam visible spectrophotometer with Absorbance Transmission and Concentration measurement modes It is a direct replacement for the earlier model 6100 This manual covers the service maintenance and repair of all units with a serial number greater than 5000 For the service maintenance and repair of units with serial numbers less than 5000 please refer to the 6300 Mark 1 Service Manual 3 2 Sub Assemblies The model 6300 spectrophotometer can easily be broken dow
19. sud 329109 4 27 8 90191 1g Pos 8 z 30 0 1 slam 2721 2775 SILON 55 S 5 eanisues 21 1 popiaosd Heq 124 one s 5 10 1 10 2 10 3 10 4 10 5 Section 10 Assembly Diagrams 6300 Final Assembly 630 503 6300 Lower Case Assembly 630 510 6300 Top Case Assembly 630 010 6300 Optics Assembly 630 508 6300 Rear Panel Assembly 6630 012 Jenway 6300 Ser Man 51 AVN al 861161 retz al 86506 Y 0S 0 9 869062 PAP VF JWS LUN SATONV SX3LIWVIC ASY 14000 NI 1542 0 5 uo SSjwuad 4 13 4 10043149 WW NI 9 30 asn 40 ou snw s 5141 3795 LN3WN I 1991940 903 SI lI LING 114 0G 5 14 01 AAYVSSJJJNNN OL J LON DNILYA 114 LNAOW ONILVAD OL ANY ALY ld DOVE OL NILYX ANDE
20. 0N 200 620 020 109 4401 280 200 602 pousissy 009 pousissy JON 802 ANT 060 200 100 1001 680 200 902 WOOT 916 100 604 pousissy 10N 09 pousissy 804 WI 118100 09 TAT 872 100 104 pousissy 022102 01 9 100 904 001 885 100 102 ION SOA 086 100 002 AUT 760 200 704 4701 660 200 104 poudissy JON 004 a pam3yuop 200 265 NOTED 082 090 O6SWWZWU Std 12 10 N SST LLO BN 905 059 UI 1591 SE 1201109 51 141145 Jo yooyo ay 10106 51165 0990 401055 10 5109056 01 24 08 pue pyg 904 01 115 09 2420 2022004 19 BuLnvsp ZUJA E 905 069 324 qof jo 5122452 TOISW 5101281151 2125 UO MOTO LOS 069 raaf Ajquiasse ST Jo spodse 151 8 0 8 I 905 049 41910955 1032924 T 0069 SILON 55 30 13548 28 11116 261828 11518 1191 808339044 8888 sper anes diese 011 AVMNAL OFF RIFE 044 sero rave waa lt 5 ASY 18000 41 _
21. SK5 and PL106 to the membrane keypad Unscrew the four screws and the PCB can be removed The display module and microprocessor PCB should be treated as a pair and replaced together Removing the display from the PCB should not be attempted Detector PCB The detector PCB is mounted vertically at the far right hand side of the lower chassis It is easily removed by unscrewing the two screws recessed in the top of the metal mounting block SK4 on the power supply PCB should be removed and the ribbon cable pulled back under the monochromator it may be necessary to lift the monochromator to complete this task Remove the two screws and spacers that hold the lens block take care not to rotate it as the detector is mounted in a recess inside the block and can easily be broken off Remove the last screw with its nut and washer to enable the electrostatic screen to be removed from the PCB The Detector PCB stores detailed calibration data relating to the optics of the unit it is fitted in replacing the detector PCB without a full re calibration will invalidate the quoted specification Monochromator The monochromator is located across the front of the lower case It is a sealed unit and breaking the seals will Jenway 6300 Ser Man 36 invalidate warranty Before proceeding with replacement ensure the unit is in a clean dust and humidity free area Remove the four screws from the base plate two at the front one at the far right hand sid
22. acts as unregulated supply for lamp solenoid and fan supplies measure between and Star 2 on power supply PCB DAC Supply 10V dc supplies 10 generated on the RS232 Interface IC101 on the Display PCB measure between pins 2 positive and 15 OV on 1 101 the Display PCB 10V dc generated on the RS232 Interface IC101 on the Display PCB measure between pins 8 negative and 15 OV IC101 the Display PCB Jenway 6300 Ser Man 12 2 4 Signal Levels Also see Section 7 2 Shutter and Filter and 8 3 Energy Levels All analogue signal processing is dealt with on the Detector PCB The output from the detector is shown in the Diagnostics mode as a Voltage in mV For more information see Section 5 2 Detector Circuit and Section 7 1 The Diagnostics Mode This voltage display can be used to check lamp energy ageing the correct functioning of the IR Stray Light filter as well as the Dark Shutter 320nm Energy In the Diagnostics Mode see Section 7 1 Diagnostics Mode set the wavelength to 320nm close the Dark Shutter by pressing the right arrow key Allow the mV reading to stabilise and record the stable value Open the dark shutter by pressing the right arrow key and the mV reading must increase by more than 4mV from that recorded above If not the lamp should be changed if it still has not improved then the condition and alignment of the optical components should be checked D
23. aff in __ 10N 809 LECER M 109 600 100 0 500 909 41001 680 600 909 5 2 8 600 OND 100 ON 600 onp 100 839 096 100 009 085 100 002 EME 100 604 poudissy JON 908 OFE 100 804 pougissy 10N soa UAS 58 100 104 580 100 HOT 96 100 904 AML 01 200 51 895 100 04 pou issy pod HOI 20 010 108 8 504 960 700 009 491 060 200 4002 558 100 104 pou issy 004 200 50 TOPA Joyoedes 087 090 2154 06 255 092 210 190915 351 2291099135 205 059 52194 009295 asem 4 Jo 0 24 134541 UMOYS P TOTA 1089 PS wontsod u 329105 0 OAS Zursnoy oyu sud uF 069 Ja IAL Aiquiasse pue suonsansur 1 2 505 069 NAON Afquiosse Jo sjodse now 5 324102 151
24. amage the component and will invalidate the warranty 1 103 is to 8 line address decoder that can select the peripheral devices on the data bus being the keypad interface through IC104 or the display driver IC200 The watchdog circuit IC100 monitors the activity of the processor while IC101 generates the correct levels for the RS232 output The four amplifiers in IC300 form the D to A converter which generates the analogue output Jenway 6300 Ser Man 25 Section 6 Software and Operation 6 0 Warning 6 1 Start Up Routine 6 2 Photometrics 6 3 Concentration Jenway 6300 Ser Man 26 6 0 Warning This section gives an overview of the software functions it is not meant to be a detailed analysis of the software routines or code It must not be treated as a substitute Instruction Manual its purpose is to enable basic navigation through the operation and set up of the 6300 spectrophotometer sufficient to verify basic operation 6 1 Start Up Routine When the power to the unit is switched on a self test routine is activated During these start up tests the following parameters are checked and must be passed before operation can continue Dark Level Test The light beam is blocked by the dark shutter solenoid switching in This test checks that the output of the detector is below a threshold level when there is no light falling on it The test will be failed if the sample chamber lid is left open during the start up ro
25. and check that the reported value is now correct 8 6 A to D Calibration The A to D converter should only be calibrated by engineers who have been trained on this aspect of servicing by Jenway Limited Equipment Required A certified voltage calibrator with a resolution of 0 1mV and a range up to at least 4 0000V A lead for connecting the calibrator to pins 1 negative and pin 3 positive of SK2 on the detector PCB 8 pin Molex type connector Access the Detector PCB by removing the top case assembly as described in Section 8 2 Dismantling Remove the jumper from pins 3 and 4 on SK2 on the Detector PCB and fit the lead connected to the calibrator Switch the calibrator on and select a negative output or reverse the contacts Select the A to D calibration mode by turning the unit on with the Print key depressed The lower display will change to prompt for specific input levels and the upper display will show the relevant mV output The initial prompt is for 1 set the calibrator to give an input signal of lmV let the upper display settle and then press the enter key Then the prompt changes to 20mV set the calibrator to 20mV let the upper display settle and then press the enter key Continue responding to the prompts in this way for 39mV 200mV 390mV 2000mV and 3900mV Note that the 39mV and Jenway 6300 Ser Man 44 390mV levels repeated as these cross over poin
26. ark Current Set wavelength to 320nm Dark Shutter closed IR stray light filter closed Voltage Display should be zero 6mV If not and no light leaks are obvious damaged seals around sample chamber lid not closing fully damaged or poorly fitted casework etc then the detector or detector PCB may be faulty 720nm Output Set wavelength to 720nm Dark Shutter open IR stray light filter open Voltage Display must not be greater than 3600mV If greater than 3600mV check lamp lamp supply voltage other power supply levels and detector PCB Jenway 6300 Ser Man 13 2 5 Error Codes See also Section 3 1 and 3 2 of the Instruction Manual A number of Error Codes are generated that relate to various fault conditions these are detailed below with a brief description of some of the most common causes for these errors Err 1 Dark level too high during a functional calibration In normal operation the dark shutter closes during an operator instigated calibration sequence to ensure that the detector output is below a threshold level The calibration is aborted and Err 1 indicated if the detector output is above this threshold level The most likely cause is that the sample chamber door has been left open or was opened during the calibration sequence it may also occur due to a faulty detector PCB Err 2 Light level too low during a functional calibration This may be caused by the sample calibrant being too optically dense dark i
27. be seen to decrease towards zero Keep pressing the down or up arrow key until the white zero order light is positioned exactly and symmetrically across the exit slit Press the print key to switch the Infra red filter out of the light path and check using the white card that this light passes the sample chamber and onto the detector lens With the zero order light passing through the sample area onto the detector press the lt CAL gt key twice and ensure that the wavelength display reads zero Replace the black monochromator cover tighten the two retaining screws and place the top half of the case on the base taking care not to trap any cables Press the up and down arrow keys and check to see if the mV reading can be increased as the wavelength changes If the display goes over range 1 Press the left arrow key to reduce the lamp brightness Jenway 6300 Ser Man 39 17 mV reading be obtained within 2nm of the zero point calibrated by this procedure then press the lt CAL gt key twice when this peak mV reading is obtained If the peak reading is obtained at a wavelength greater than 2nm or less than 2nm then the above alignment procedure should be repeated When OK replace the four screws in the base section 18 wavelength calibration must now be carried out see Section 8 5 and then a full performance verification as detailed in Section 8 8 Grating Position Sensor Operation and adjustment
28. bove can be supplied as Calibration Filter Sets order part numbers 035 088 Where filters are not available the following reagents may be used 8 8 1 Holmium Perchlorate 5 w v solution of Holmium Oxide in 1 4N Perchloric acid this will give absorbance maxima at 361 4 416 1 451 1 485 3 536 5 and 640 5 8 8 2 Potassium Dichromate 100 0mg I in 0 005M Sulphuric Acid use the Sulphuric Acid as the blank This will give Absorbance values of 1 071 at 350nm 0 484 at 313nm Potassium Dichromate 50 0mg I in 0 005M Sulphuric Acid use the Sulphuric Acid as the blank This will give Absorbance values of 0 536 at 350nm 0 242 at 313nm 8 8 3 Sodium Nitrate 505 1 in deionised water should give less than 0 1 transmittance at 340nm All these solutions are hazardous and the manufacturer suppliers safety precautions should be carefully followed at all times in preparation use and storage 8 8 4 Wavelength Verification Equipment Required A certified wavelength standard i e Holmium Oxide Filter Holmium Perchlorate Solution etc See Section 8 8 1 Turn the unit on and allow the Start Up tests to complete then allow 15 minutes for the instrument to warm up Use the up and down arrow keys to select a wavelength about 10nm below the certified wavelength of the filter or standard to be used Jenway 6300 Ser Man 46 Select absorbance transmission modes using right left arrow keys depending on whe
29. bration Equipment Required A certified wavelength standard i e Holmium Oxide Filter Holmium Perchlorate Solution etc See Section 8 8 1 Wavelength calibration can be carried out in the Diagnostics Mode using the wavelength zero order offset function Do not carry out the following procedure without a suitable certified wavelength calibration standard Turn the unit on and allow the Start Up tests to complete Use the up and down arrow keys to select a wavelength about 10nm below the certified wavelength of the filter or standard to be used Select the absorbance or transmission modes using the right or left arrow keys depending on whether an Absorbance or transmittance standard or filter is being used Jenway 6300 Ser Man 42 Press the CAL key to set the display to 0 000 5 100 T Insert the filter or standard in the sample chamber and close the lid Press the up arrow key to increase the wavelength by and check that an Absorbance value has increased or a Transmission value has decreased Repeat the above until the first Absorbance value decreases or the first Transmission value increases At this point press the down arrow key and check that the previous value is attained again Then record this wavelength as the reported peak Repeat the above and check that the same value is reported NOTE 1 If it is known that there are other peaks closer than 10nm to the certified peak then the start wavelength sh
30. dealt with on the Detector PCB The output from the detector is shown on the Diagnostics Screen as a Voltage in mV For more information see Section 5 3 Detector Circuit and Section 7 1 The Diagnostics Mode This voltage display can be used to check lamp energy ageing the correct functioning of the IR Stray Light filter as well as the Dark Shutter Jenway 6300 Ser Man 41 320nm Energy In Diagnostics Mode see Section 7 1 Diagnostics Mode set the wavelength to 320nm close the Dark Shutter by pressing the right arrow key Allow the mV reading to stabilise and record the stable value Open the dark shutter by pressing the right arrow key and the mV reading must increase by more than 4mV from that recorded above If not the lamp should be changed if it still has not improved then the condition and alignment of the optical components should be checked Dark Current Set wavelength to 320nm Dark Shutter closed IR stray light filter closed Voltage Display should be zero If not and no light leaks are obvious damaged seals around sample chamber lid not closing fully damaged or poorly fitted casework etc then the detector or detector PCB may be faulty 720nm Output Set wavelength to 720nm Dark Shutter open IR stray light filter open Voltage Display must not be greater than 3600mV If greater than 3600mV check the lamp lamp supply voltage other power supply levels and detector PCB 8 5 Wavelength Cali
31. e Do not look directly at the light source or allow the light beam to fall directly on the eyes switch off or dim the lamp as described in Jenway 6300 Ser Man 6 procedures whenever possible and wear eye protection at ALL times The lamp gets very hot when in use always allow time for it to cool down before removal Always wear cotton gloves when removing a faulty lamp and replacing with a new one Finger marks dust and condensation can quickly destroy sensitive and expensive optical components always wear cotton gloves when the optical bench is uncovered and handle any components by their edges only Never touch optical surfaces Do not remove optical covers unless the unit is in a clean dust and condensation free environment Many of the reagents solutions and standards used for maintenance and calibration are corrosive or hazardous ensure all precautions supplied with them are followed where there is any doubt request a MSDS Material Safety Data Sheet from the supplier This instrument can be used for analysing a broad range of samples do not handle them unless you are qualified to do so Ensure that the instrument has been correctly decontaminated before working on it specifically in areas where the instrument may have been used for clinical biological corrosive or radioactive samples 1 4 Standards amp Certification No adjustments should be made to this instrument unless the test and measurement equipmen
32. e Chamber Lid 630 013 Microprocessor PCB Micro processor PCB EPROM Programmed 630 510 Lower Case Assembly Torroidal transformer assembly Large rubber feet Cooling fan 12V 60mm 630 508 Monochromator Assembly Tungsten halogen lamp base Tungsten halogen lamp Solenoid 12 IR Filter 6300 Chassis mount optocoupler 630 506 Detector PCB Detector PCB assembly 6300 470uF 16V radial electrolytic capacitor Photodetector 6300 Voltage regulator 791 05 Voltage regulator 7805 630 504 Power Supply PCB Main power supply PCB 1000uF 25V radial electrolytic capacitor 53 005 024 002 112 020 027 006 115 009 124 009 125 Section 009 123 016 021 062 241 017 050 11 10 Jenway 6300 Ser Man Bridge rectifier 4700uF 40V radial electrolytic capacitor L4960 voltage regulator 25 way D socket 4mm socket red 4mm socket black 640 006 Rear Panel Assembly Mains input socket 2A fuse 20mm for 220V supply Lamp Panel Retaining Screw Switch rocker 2p 54
33. e cursor on the bottom menu bar under the T icon Fill a cuvette with the deionised water used to make up the solutions and place it in the sample chamber Or for a filter standard use the blank filter supplied by the manufacturer Press the CAL key and ensure the reading is 100 0 Insert the stray light filter or solutions as above and ensure that the reading is within the specified tolerance of the instrument PLUS the tolerance of the filter solution used Jenway 6300 Ser Man 49 9 1 9 2 03 9 4 9 5 9 6 Section 9 Circuit Diagrams Power Supply Schematic 630 504 Power Supply Layout 630 504 Detector PCB Schematic 630 506 Detector PCB Layout 630 506 Microprocessor PCB Schematic 630 013 Microprocessor PCB Layout 630 013 Jenway 6300 Ser Man 50 518 856028 11 221628 1251 19 2193455 829 4 ZAW BBLS OLIAVMNSSL F gt 01655 905029 247472 3199 29 96 32 22 51 r s STON 31725 LON 08 7580 z ue HM Wa3N39 02 api fom GEN 40 379555 2 gt ee ees 9499 3504 51
34. e in front of the detector PCB the other on the far left hand side Carefully lift the monochromator assembly unplugging the connectors SK1 and SK9 without straining the cables The monochromator can now be removed Replacement is the reverse of dismantling but ensure that all cables are carefully fitted in the appropriate recesses so that they are not crushed when screwing the unit down When fitting a replacement unit ensure a full calibration is run so that the new calibration data for the new monochromator is stored See Section 8 5 8 4 and 8 7 Power Supply PCB With the monochromator removed as above the power supply PCB is easily removed by undoing the four screws holding it to the lower case assembly SK3 to the transformer and SK1 to the fan should be removed before lifting it out of the lower case Cooling Fan Carry out the above procedures to enable the fan to be lifted out of its recess in the lower case Ensure the position of the retaining spring and the direction of the airflow are noted for re assembly Transformer Remove SK3 from the power supply PCB and the push on connectors for the cables that go to the mains switch and inlet filter assembly Then remove the transformer by undoing the single bolt through the centre that holds it to the moulded bracket in the base assembly 8 3 Optical Alignment This procedure can irreversibly affect the performance and operation of the instrument Please read the following warnings
35. ernational Standard Methods as in beer colour measurements to EBC standards etc A range of units can be displayed against the top digital Concentration display The required units can be selected by moving the cursor with the right or left arrow keys along the bottom menu bar until it is under the UNITS icon The Up or Down arrow keys then be used to display in turn ppm mg l g l 90 or blank no units displayed When the required unit is displayed simply move the cursor back to xF or CONC To calibrate the concentration range against a known standard move the cursor beneath the CONC icon using the right or left arrow keys Insert a cuvette or test tube containing the known standard solution into the sample chamber close the lid and press the CAL key The CAL icon on the display will flash and the up or Jenway 6300 Ser Man 29 down arrow keys be used to adjust value displayed on upper digital display to the actual value of the standard being used When this value has been set a further press of the CAL key completes the calibration against this standard solution Cuvettes or test tubes containing samples can then be inserted in the sample chamber the sample chamber lid closed and readings taken directly from the display During the above calibration procedure the software calculates the factor required to multiply the Absorbance by to achieve the linear relationship with the concentration of the standard u
36. following 630 508 Optics Assembly 630 504 Power Supply PCB 630 506 Detector PCB 060 311 Cooling Fan 010 040 Torroidal Transformer Optics Assembly includes the following 012 075 Tungsten Halogen lamp 032 005 12V Solenoid 630 516 IR Filter Rear Panel Assembly includes the following 016 021 2A Fuse 20 x 5mm 017 050 Mains Switch 009 123 Mains Input Socket 11 23 Power Supply Voltages Also see Section 9 Circuit Diagrams Before commencing more complex fault finding it is important to check all the internally generated supply voltages are correct The following list is a useful guide to help quickly check these are functioning correctly Not all the points where these voltages can be measured are given and where the voltage is stated as unregulated variations may occur In general regulated supplies should vary by no more than 5 from their nominal value Tungsten Lamp Supply 12V dc regulated and set by measure at SK9 pin 5 with respect to SK9 pin 6 on the power supply PCB and at the terminals on the lamp base with the lamp fitted Solenoid and Fan Supplies 12V dc regulated and pre set measure at SK1 pin 1 with respect to SK1 pin 2 on the power supply PCB and on the solenoid and fan terminals Digital Supply 5V dc regulated and pre set measure at SK5 pin 1 with respect to SK5 pin 2 or on 5 1 pin with respect to SK1 pin 2 on the Display PCB Stepper Motor Drive 30V dc unregulated also
37. ium Dichromate solution See Section 8 8 2 Turn the unit on and allow the Start Up tests to complete then allow 15 minutes for the instrument to warm up Use the up or down arrow keys to select a wavelength at which the filter or solution is certified For the Potassium Dichromate solution use the Sulphuric Acid solution as a blank See Section 8 8 2 if the filter set includes a zero filter use this as the blank if not set the blank zero absorbance or 100 transmittance with an empty sample chamber Insert the blank ref above paragraph into the sample chamber and close the lid Press the CAL key and ensure the display reads 0 000 ABS or 100 T Remove the blank from the sample chamber Insert the certified filter or Potassium Dichromate solution and check that the reading is within the specified tolerance of the instrument PLUS the tolerance of the filter solution used Repeat this for other filters or solutions and at other specified wavelengths as necessary Jenway 6300 Ser Man 48 8 8 6 Stray Light Verification Equipment Required Certified Stray Light Filters or Sodium Nitrate Solution or Sodium Iodide Solution See Section 8 8 3 Turn the unit on and allow the Start Up tests to complete then allow 15 minutes for the instrument to warm up Select a wavelength at which the filter or solution is certified 340nm for Sodium Nitrate Select the Transmission mode by using the right and left arrow keys to move th
38. lectric shock connect the power cable and depress the right arrow key gt while turning the power switch on 5 The display should show mV reading on top row followed by the wavelength on the 2 row and T at the bottom left hand side 6 Pressing the right arrow key again gt should operate the solenoid in front of the exit slit assembly Press this key to ensure that the shutter is in the open position light is passing through the exit slit Jenway 6300 Ser Man 38 10 11 12 13 14 15 16 Pressing left arrow key will dim light source This should always be done when looking at or towards the light source For now ensure it is pressed to give maximum light output Follow the light path from the lamp through the entrance slit assembly on to the grating Use a narrow strip of white card to help show the position and shape of the light beam DO NOT TOUCH ANY OPTICAL COMPONNENTS Move the card in an arc in front of the grating from the exit slit assembly through the incident light beam and towards the torroidal transformer at the back of the instrument While doing this identify the bright coloured spectrum 1 order the white incident light beam the white zero order light beam and then the dimmer coloured spectrum 259 Press the up or down arrow key to move white zero order light beam towards the exit slit Usually the down arrow key The wavelength displayed should
39. must be carried Jenway 6300 Ser Man 32 out after this procedure has been used Also see Section 8 3 Optical Alignment 1 Press right arrow key gt while turning power switch 2 The display should show a mV reading on the top row followed by the wavelength on the 2 row and T at the bottom left hand side 3 Pressing the right arrow key again gt should operate the dark shutter solenoid Press this key to ensure that the shutter is in the open position the position that gives a maximum reading on the display 4 Press the down arrow key until the wavelength display reads 0 0 Press the left arrow key to dim the lamp 5 Use the up and down arrow keys to move in 0 5nm steps either side of zero and identify at which wavelength a peak mV reading is obtained Note this reading 6 Pressing the CAL key will re set this reading to zero and the CAL symbol on the display will flash While it is flashing carry out step 7 or step 8 7 Pressing the CAL key again will clear wavelength display offset noted in step 5 to zero 8 The offset noted in step 5 or an alternative correction see Section 8 5 Wavelength Calibration can be entered before pressing the CAL key by using the up or down arrows to set the display to the required offset 9 If step 7 was used a wavelength calibration must be carried out in both cases a performance verification as detailed in Section 8 8 must be carried out Jenway 6300 Ser Man 33
40. n into sub assemblies for the purposes of repair or replacement All the PCBs are easily removed see Section 8 2 Dismantling The monochromator is also a replaceable sub assembly There is a range of sampling accessories that can be easily fitted and removed from the sample chamber See Section 2 2 for details of the main sub assemblies and Section 11 for details of other spare parts The following paragraph lists the sampling accessories available 3 3 Accessories The following sampling accessories are available where necessary additional service information for these accessories is available on request The development of other sampling accessories 15 continuous please check current brochures or www jenway com for up to date information 632 001 External Sipper Pump 230V 632 031 External Sipper Pump 115V 634 001 4 Position Cell Changer 630 020 Test Tube Holder 13mm diameter 630 021 Test Tube Holder 25mm diameter 630 022 Test tube Holder 16mm diameter 630 005 20 to100mm Single Cell Holder 648 001 Water Heated Single Cuvette Holder Jenway 6300 Ser Man 18 Other accessories include 543 001 External 40 Column Printer 542 009 Interface Cable Kit 630 028 Dust Cover 037 201 Water refrigerant Circulator for use with 648 001 3 4 Outputs The 6300 has both analogue and RS232 outputs Details of the level of the analogue output for the different ranges that may be selected on the instruments is given in Secti
41. on 6 3 of the Instruction Manual Pin configuration for the RS232 socket is given in Section 6 2 of the Instruction Manual Section 6 1 of the Instruction Manual gives details of the various ASCII codes that may be transmitted to the 6300 to enable complete remote control from a terminal or PC Jenway 6300 Ser Man 19 Section 4 Optical Description 4 1 Light Source 4 2 Grating 4 3 Shutter and Filter 4 4 Signal Detector Jenway 6300 Ser Man 20 4 1 4 2 4 3 4 4 Light Source The 6300 uses a single Tungsten Halogen lamp to cover the full wavelength range from 320 to 1000nm This is a pre focussed lamp selected for the accurate position of the filament making re alignment after replacement unnecessary The use of lamps other than those supplied by Jenway Ltd part number 012 075 will cause error codes to be displayed and result in erroneous results See Section 4 2 of the Instruction Manual Grating From the lamp the light passes through a slit and lens assembly then onto a flat holographic grating with 1200 lines per millimetre The grating is directly coupled to the stepper motor which is under microprocessor control An opto coupler mounted just behind the grating acts as an end stop when the sensor vane connected to the grating mount blocks the light path across it Shutter and Filter The diffracted spectrum then passes through a further slit and lens arrangement after which the light beam can be
42. or Jenway 6300 Ser Man 5 5 1 5 2 5 3 6 6 0 6 1 6 2 6 3 7 7 1 7 2 7 3 7 4 8 8 1 8 2 8 3 8 4 8 5 8 6 8 7 8 8 Electronic Description Power Supplies Detector Circuit Microprocessor and Display Software and Operation Warning Start Up Routine Photometrics Concentration Diagnostics Diagnostics Mode Shutter and Filter Control Lamp Control Zero Order Calibration Maintenance Routine Maintenance Dismantling Optical Alignment Energy Levels Wavelength Calibration A to D Calibration D to A Calibration Performance Verification Jenway 6300 Ser Man Section 9 Circuit Diagrams 9 1 9 2 9 3 9 4 9 5 9 6 Supply Schematic 630 504 Power Supply Layout 630 504 Detector PCB Schematic 630 506 Detector PCB Layout 630 506 Microprocessor PCB Schematic 630 013 Microprocessor PCB Layout 630 013 Section 10 Assembly Diagrams 10 1 10 2 10 3 10 4 10 5 6300 Final Assembly 630 503 6300 Lower Case Assembly 630 510 6300 Top Case Assembly 630 010 6300 Optics Assembly 630 508 6300 Rear Panel Assembly 630 012 Section 11 Spare Parts List 11 01 11 02 11 03 11 04 11 05 11 06 11 07 11 08 11 09 Packed Instrument Top Case Assembly Microprocessor display PCB Lower Case Assembly Lamp Housing Assembly Monochromator Assembly Detector PCB Power Supply PCB Rear Panel Assembly Jenway 6300 Ser Man 11 Abo
43. ould be moved closer to the certified peak just past any others 2 If the Absorbance display goes over range during the test then carry out the Calibration at the initial wavelength with the filter or standard in the sample chamber Calculate the adjustment required to correctly align the reported figure with the certified value from the following Certified Value Reported Value Correction factor can be negative or positive maximum correction permissible is 3 0nm Switch the unit off and re start it in the Diagnostics Mode by holding down the right hand arrow key lt gt gt while turning power on The display should show a mV reading on the top row followed by the wavelength on the 2 row and T at the bottom left hand side Pressing the right arrow key again gt should operate the dark shutter solenoid Press this key to ensure that the shutter is in the open position the position that gives a maximum reading on the display Press the down arrow key until the wavelength display reads 0 0 Press the left arrow key to dim the lamp Use the up and down arrow keys to set the display to the correction factor calculated above observe polarity Jenway 6300 Ser Man 43 Pressing CAL key again will enter this value into memory as new wavelength calibration offset Switch the unit off and then on again allowing the Start Up tests to be completed re check the certified wavelength calibration standard as above
44. output against internally generated reference voltages See Section 8 6 View Start Up Routine Hold the lt Cal gt key depressed while turning on the power This enables the detector output and grating position to be monitored on the display during the Start Up Routine Jenway 6300 Ser Man 15 A to Calibration Hold lt Print gt key depressed while turning on the power This enables the A to D converter on the detector PCB to be calibrated against an external precision voltage source See Section 8 5 Diagnostics Mode Hold the lt right arrow gt key depressed while turning on the power The main display shows the signal from the detector in mV and the resolution of the wavelength display is increased to 0 5nm See Section 7 1 Further special key functions are invoked in the diagnostics mode as follows lt right arrow gt Further presses of this key will toggle the dark shutter open and closed See Section 7 2 lt left arrow gt Alternate presses of this key will reduce the lamp voltage to 5V dim and then return it to 12V See Section 7 3 lt Cal gt This key is used to set the wavelength display to 0 0 with the grating in a position where white light zero order is reflected on to the detector A second press of this key is required to confirm the setting before resuming the normal diagnostic functions See Section 7 4 lt print gt Alternate presses of this key will toggle the Infra red filter in and out of the light
45. ransmission measurement mode similarly moving it under the ABS icon will enable the Absorbance measurement mode These two modes are calibrated by simply inserting a cuvette or test tube containing a blank solution in the sample chamber closing the lid and pressing the CAL pushbutton The CAL icon on the display will flash while Jenway 6300 Ser Man 28 the calibration sequence is in progress when it stops main digital display will be set to 100 0 T or 0 000 ABS automatically Sample measurement can then be carried out by inserting a cuvette or test tube containing the sample solution into the sample chamber closing the lid and recording the value on the main display 6 3 Concentration Moving the cursor under the CONC icon on the bottom menu bar enables the concentration measurement mode NOTE Before selecting this mode a blank calibration must be carried out in the Absorbance mode at the wavelength to be used for the concentration measurement When the concentration mode is selected two further icons xF and UNITS appear on the bottom menu bar A linear relationship between Absorbance and Concentration is assumed for all measurements This enables concentration measurements to be made against a standard solution CONC mode or by entering a factor which adjust the slope of the straight line relationship xF mode The latter is also often used to improve the sensitivity of comparative colour measurements in a number of Int
46. rt up tests to reduce the light level for accurate zero order detection it can also be manually instigated by pressing the left arrow key when in the Diagnostics Mode Solenoid and Fan Supply The output of the switch mode regulator is pre set to 12V The output to the fan is via 5 1 pin and 2 The output to solenoid 1 Dark Shutter is via SK9 pins 1 and 2 The output to solenoid 2 IR Stray Light Filter is via Sk9 pin 3 and 4 5V Digital Supply The other transformer secondary output is half wave rectified and regulated to 5V by the linear regulator REG 2 The output is distributed via SK5 pin 1 Ancillary Supplies to the end stop opto coupler are connected via SK2 pins 1104 The RS232 output accessible through the rear panel on SK6 is fed from the microprocessor PCB via SKS and 2 TEA3717DP the stepper motor drivers controlled from the microprocessor PCB via SK5 pins 9 to 12 with outputs to the motor on SK2 pins 5 to 8 Jenway 6300 Ser Man 23 5 2 Detector Circuit Detectors and Amplifiers The current through the detector D1 S1133 type fitted is proportional to the incident light acts a current to voltage converter the gain being set by the feedback resistors in the T network In normal operation pins 3 and 4 of SK2 are linked so the signal passes to the three amplifiers of IC1 IC1b is set for unity gain has a gain of 10 1 14 a gain of 100 A to D Con
47. sed The Factor xF mode is automatically updated with this value which can be viewed by moving the cursor using the right or left arrow keys along the bottom menu bar until it is under the xF icon For future assays this factor could be used instead of the standard solution but good practice and standard operating procedure sop should be observed and the accuracy of this factor be verified on a regular basis If a factor is known or supplied in a sop then this can be entered directly in the Factor xF mode by moving the cursor using the right or left arrow keys along the bottom menu bar until it 15 under the xF icon The up or down arrow keys can then be used to adjust the value of the factor on the lower digital display from 0 to 9999 when the correct value has been set move the cursor back under the CONC icon using the left arrow key Cuvettes or test tubes containing samples can then be inserted in the sample chamber the lid closed and readings taken directly from the display Jenway 6300 Ser Man 30 Section 7 Diagnostics 7 1 The Diagnostics Mode 7 2 Shutter and Filter Control 73 Lamp Control 7 4 Zero Order Calibration Jenway 6300 Ser Man 31 7 1 T2 7 3 7 4 The Diagnostics Mode The Diagnostics mode is accessed using the following Special Key function hold down the right arrow key lt gt gt while turning the power on The main display now shows the linearised signal from the detector in mV and the re
48. solution of the wavelength display has increased to 0 5nm Shutter and Filter Control Once the diagnostics mode has been activated further presses of the right arrow key lt gt gt will toggle the dark shutter alternately between the open and closed positions With the covers still on the instrument the actual position of the dark shutter can be determined by the main display that will in general have a very low zero reading when the shutter is closed and a very high reading when the shutter is open Once the diagnostics mode has been activated alternate presses of the print key will toggle the Infra red filter in and out of the light path A similar effect to the above can be used to determine the actual position of the filter when the instrument covers are in place of course the difference between the in and out readings will be greater at higher wavelengths Lamp Control Once the diagnostics mode has been activated alternate presses of the left arrow key lt lt gt will reduce the lamp voltage to 5 dim and then return it to 12V This function is automatically used in the start up tests to accurately detect the zero order position For safety reasons it should also be used at all times when working on the instrument with any covers removed such that the lamp is exposed Zero Order Calibration Warning This procedure can be used to re set the stored wavelength calibration data so a new wavelength calibration
49. t Err 6 No dark level found in start up routine Leaving the sample chamber open during the start up routine or opening it once the procedure has started can cause this error It can also indicate the failure of the dark shutter solenoid the solenoid drive or the associated control circuits Err 7 Grating position sensor not detected This error occurs during the wavelength calibration procedure in the start up routine and is generated at the beginning of this procedure The grating is driven in a clockwise direction until the attached vane breaks the path of an opto coupler This is effectively the end stop and the point from which auto calibration will always start Failure to detect this point may be due to a faulty opto coupler wavelength drive from the power supply PCB a faulty motor or mechanical coupling 2 6 Special Key Functions There are a number of special key functions for use by trained engineers do not use them unless you are fully conversant with all the procedures these invoke Power On Reset Hold the lt Enter gt key depressed while turning on the power This clears the operator set parameters held in non volatile memory and is useful in correcting many software conflicts As this procedure by passes the start up routine the instrument must be re booted before use Analogue Output Calibration Hold the lt up arrow gt key depressed while turning on the power This enables fine calibration of the analogue
50. t signal source or filters to be used have a current calibration certificate that is traceable to national or international standards and that it is known that this test equipment is currently performing to the certified standards All solutions and reagents should be fresh and within any stated shelf life with a certificate of analysis 1 5 Ordering Spares When ordering spare parts as detailed in this manual please quote the Part Number and Description These items should be ordered from the original supplier of the equipment or your local Jenway Limited Distributor Jenway 6300 Ser Man 7 1 6 Returning Items Should it be necessary to return any item for any reason then this should be done through the original supplier of the equipment or your local Jenway Limited Distributor 1 7 Contacting Jenway Limited Before contacting Jenway Limited please check our web pages for any information or updates that may be helpful to you www jenway com Emails should be sent sales jenway com Fax 44 1371 821083 Phone 44 1371 820122 Please note no items can be returned to or will be accepted by Jenway Limited without a Returns Authorisation number RA number and a completed Safety Clearance and Decontamination certificate Jenway 6300 Ser Man Section 2 Quick Reference 2 0 About Quick Reference 2 1 Specification 2 2 Main Sub Assemblies 2 3 Power Supply Voltages 2 4 Signal Levels 2 5 Error Codes 2 6 Special
51. t may also be caused by lamp failure as well as the use of plastic or glass cuvettes at wavelengths where these materials will absorb a high proportion of the light energy Err 3 Standard out of range in a concentration measurement This may be because the standard is too optically dense at the wavelength selected or it may be too similar to the blank Dillution of the sample may be applicable for the former or the wavelength for the later method may require adjustment Err 4 No zero order white light found during wavelength calibration in start up routine This error is given at the end of the wavelength calibration routine indicating that the test was partly successful see Section 6 1 Start Up Routine Hence this error is normally caused by low energy levels due to lamp ageing or contamination deterioration of other optical components Also ensure that a sample or cuvette has not been left in the sample chamber during the start up tests and that there is no splashing or spillage on the exit and detector lenses Err 5 No zero order white light found during wavelength calibration in start up routine This error is given during the wavelength calibration routine indicating that even the initial threshold level was not achieved Possible causes are incorrect Jenway 6300 Ser Man 14 lamp fitted cuvette sample or other obstruction in the light path through the sample chamber as well as a possible optical component failure or misalignmen
52. ther an Absorbance or transmittance standard or filter is being used Press the CAL key to set the display to 0 000 5 100 T Insert the filter or standard in the sample chamber and close the lid Press the up arrow key to increase the wavelength by and check that an Absorbance value has increased or a Transmission value has decreased Press the up arrow key again to increase the wavelength by Inm and check that an Absorbance value has continued to increase or a Transmission value has decreased further Repeat the above until the first Absorbance value decreases or the first Transmission value increases At this point press the down arrow key and check that the previous value is attained again Then record this wavelength as the reported peak Repeat the above and check that the same value is reported NOTE 1 If it is known that there are other peaks closer than 10nm to the certified peak then the start wavelength should be moved closer to the certified peak just past any others 2 If the Absorbance display goes over range during the test then carry out the Calibration at the initial wavelength with the filter or standard in the sample chamber Check that the reported peak wavelength falls within the specified tolerance of the instrument PLUS the tolerance of the filter or standard used Jenway 6300 Ser Man 47 8 8 5 Absorbance Verification Equipment Required Certified Standard Absorbance Filters or Potass
53. this can be carried out as follows Remove the monochromator cover observing all the previously stated precautions Press the up arrow key to select a wavelength about 500nm and check that the grating rotates correctly Pass a piece of thick paper or card between the jaws of the opto coupler and check that when the card is inserted a voltage of approximately 5 00V is measured as above and that when it is removed this voltage drops to approximately 0 100V If these results are correct then the position should be adjusted as below if not then the opto coupler or power supply PCB may be faulty There may also be a bad connection between them Check the wiring to SK2 and that no wires are trapped under the monochromator To adjust the position press the down arrow key to set the wavelength to 50nm slacken the two fixing screws slightly and adjust the position of the opto coupler until the voltmeter reading just changes from 0 100V to 5 00V Tighten the screws and check that at 40nm the reading is approximately 0 100 and that at 5Onm it is approximately 5 00V If not repeat as above 8 4 Energy Levels Equipment Required None checked against internal settings Before proceeding with any calibration it is essential to ensure the correct functioning of the optical system this can be done very easily in the Diagnostics mode see Section 7 where the following performance should be obtained All analogue signal processing is
54. ts from one channel to the next When successfully completed the display returns to the last settings used in the measurement mode 8 7 Dto A Calibration Equipment required Voltmeter capable of reading 2 0V with a resolution of ImV The D to A calibration sets the levels of the analogue output This is carried out with the on board voltage reference at zero and 2000mV Select the D to A calibration mode by holding the up arrow depressed while the power is turned on The upper display will show dAC mV and the lower display will indicate the mV level that should be available on the Analogue output Connect a voltmeter to the analogue output on the rear panel Select a range that will display 2000mv to 0 1mV resolution The first prompt indicates an output level of 2000mV use the up and down and left and right arrow keys to adjust the actual reading on the voltmeter to 2000mV The left and right arrow keys change the output in 5mV steps the up and down arrow keys in 0 5mV steps When the correct level is reached press the enter key and the prompt moves to OmV repeat the above for this and the 2000mV levels When successfully completed the display returns to the last settings used in the measurement mode Jenway 6300 Ser Man 45 8 8 Performance Verification Equipment Required 1 Certified Wavelength Standard 2 Certified Absorbance Standards 3 Certified Stray Light Standard Items and 2 a
55. ut This Manual This manual covers the service maintenance calibration and repair of the Jenway Ltd model 6300 Spectrophotometer From serial number 5000 upwards for lower numbers please refer to 6300 Manual This manual must be used in conjunction with the Instruction Manual for this model as many of the routine maintenance procedures detailed in the Instruction Manual are not repeated in this Service Manual 1 2 Using This Manual This manual is only for the use of Engineers and Technicians who have successfully completed a Jenway Ltd approved Service Training course on the Model 6300 Spectrophotometer Updates to this manual will be circulated through the Jenway Ltd TSI Technical Service Information systems and to other registered users of this manual Please complete the form at the rear of this manual to register your copy for future updates In practice Section 2 Quick Reference and Section 8 Maintenance with the Diagrams in Section 9 and 10 will be most frequently used however it is good practice to read the complete manual initially and review it again periodically To find the information required refer to the Main Index or Index to Sections to identify the relevant Section page number required 13 Warnings amp Safe Practice Always disconnect the mains supply when any covers are removed as there are voltages present inside the unit that pose the risk of electric shock at levels that are hazardous to lif
56. utine if the dark shutter solenoid 1 is faulty electrical or mechanical or if the Detector or Detector PCB is faulty Wavelength Calibration This test checks for the zero order white light that is reflected through the sample chamber when the grating is in a position where it acts as a mirror Each time the unit is switched on this position is used as a physical reference point against which the stored wavelength calibration data is applied This test is carried out in the following manner the microprocessor instructs the grating to drive to its minimum value of 200nm This should ensure that the vane attached to the grating mount breaks the light path of the opto coupler mounted on the monochromator base plate that is set at a position of approximately 50 If it does not receive a signal from the opto coupler then error code Err 7 grating position sensor not detected is returned when the motor stops In correct operation a signal is returned when the vane reaches the opto coupler Then the microprocessor instructs the grating to rotate in the reverse direction in Inm steps for 200nm or until a signal greater than 200mV is returned from the detector this level Jenway 6300 Ser Man 27 with lamp dimmed can only be produced by white zero order light If this 200mV level can not be achieved then the grating stops after having rotated the 200nm and an error code Err 5 no peak light level found is returned
57. version Each of these amplified signals then pass into the first three channels CHO to CH2 of an 8 channel 12 bit serial A to D converter All three channels are converted and the microprocessor selects the channel that gives the best resolution without reaching saturation 32767 counts In effect this means CH2 will be selected for inputs up to 40mV for inputs up to 400mV and for inputs up to 4 0V The A to d converter requires a reference voltage of 4 096V which is generated from the 5V rail by D4 this is fed to pin 14 Ref of the A to D converter 1 2 The 1 4 maintains calibration data for the PCB see Section 8 6 A to D Calibration 5 3 Microprocessor and Display The microprocessor 1 102 is H8 325 type with 64K linear address space its architecture is register based and optimised for software written in the C language A 16 bit address bus is used with an 8 bit external data bus The 1024 bytes of internal RAM are shared equally between the internal requirements of the processor and for the storage of operator set variables The later are also stored in non volatile EPROM to enable the last settings to be restored after power has been interrupted or switched off Jenway 6300 Ser Man 24 The operating system software is stored in 64K by 8 bit EPROM IC106 This will have a label attached identifying the software version and date it was programmed Removal of this label may d
Download Pdf Manuals
Related Search