Mouse anti-EGFr - Thermo Fisher Scientific
Contents
1. Dejar secar antes de ver 10 minutos DETECCI N Y DIAGN STICO DE AVER AS Problema Soluci n El control positivo muestra una Verifique los dem s controles positivos preparados durante esa misma serie con el instrumento para inmunotinci n d bil determinar si se debe al anticuerpo primario o a uno de los reactivos secundarios comunes Aseg rese de que el portaobjetos tenga la etiqueta con el c digo de barras de Ventana correcta Si est etiquetado correctamente verifique los dem s controles positivos preparados durante esa misma serie con el instrumento para determinar si se debe al anticuerpo primario o a uno de los reactivos secundarios comunes El control positivo es negativo MARCAS COMERCIALES g g Cap Plus Clearmount Digest All HistoGrip Histomount y Histostain NBATM PicTureTM y Zymed son marcas comerciales de Zymed Laboratories Inc Ventana ES NexES y BenchMark son marcas comerciales de Ventana Medical Systems Inc z ee a e Los anticuerpos V Line son elaborados exclusivamente por Zymed Laboratories Inc y no implican ni la aprobaci n ni el aval de Ventana Medical o R Systems Inc Las empresas Zymed y Ventana no estan afiliadas asociadas ni relacionadas de manera alguna Para obtener m s informaci n vea la versi n inglesa de la ficha t cnica o vaya a www invitrogen com TIENE ALGUNA PREGUNTA SOBRE ESTE PRODUCTO DIRI2. BEABSICHTIGTER NUTZEN Fir In Vitro Diagnostischem Gebrauch Die Interpretation muss im Zusammenhang mit der klinischen Vorgeschichte des Patienten und anderen Diagnostiktests eines qualifizierten Pathologen vorgenommen werden REAGENS GELIEFERT Maus Anti EGFr ist gereinigt von Maus asciter Fl ssigkeit und verd nnt in Phosphatgepufferter Salzl sung PBS pH 7 4 und 1 igem Rinder Serum Albumin BSA mit 0 1 Sodium Azid NaN als ein Konservierungsmittel Immunogen Humanes EGFr abgeleitet aus A 431 Zellen Isotyp IgG Klon 31G7 Positive Kontrolle Brustkarzinom Erwartetes F rbungsmuster Zellmenbran und cytoplasmatisch LAGERUNG 2 8 C VORAUSSETZUNGEN F R DIE IMMUNHISTOCHEMISCHE FARBUNG Invitrogens V Line Vorverd nnung wurde speziell f r die Verwendung mit den immunhistochemischen F rbungssystemen Ventana EST NexES und BenchMark in Verbindung mit Ventana Detektionskits und Ventana Reagenz Dispensern entwickelt Invitrogen hat diesen Antik rper titriert und qualit tskontrolliert um dessen konstante und zuverl ssige Leistung zu gew hrleisten In Tabelle 1 sind die Voraussetzungen f r die immunhistochemische F rbung zusammengefasst Tabelle 1 Voraussetzungen fur die FFPE Geweben Angabe Hitze Induzierte Epitop Wiedergewinnung HIER Nicht ben tigt Enzym Verdauung Protease I Enzym Inkubation 8 Minuten Inkubationsschritte f r den prim ren Antik rper 32 Minuten
3. BEN TIGTE ABER NICHT GELIEFERTE MATERIALIEN Reagens Katalog Nr 1 HistoGrip M 00 8050 2 PBS 0 01 M PBS 00 3000 3 Mayer s Haematoxylin 00 8011 4 Zitratpuffer pH 6 0 falls f r die durch W rmeeinwirkung induzierte Wiedergewinnung der Epitope erforderlich 00 5000 5 EDTA L sung falls f r die durch W rmeeinwirkung induzierte Wiedergewinnung der Epitope erforderlich 00 5500 6 Immunhistochemische Farbungssysteme Ventana ESTM NexES oder BenchMark e Strichcode Etikett e Objekttr ger F rbeautomaten ES NexES oder BenchMark e DAB oder AEC Detektionskit e Detektionskit spezifische Software e Waschl sung e Negatives Kontrollreagenz e Fl ssigkeits Deckglas 7 Pr parierungsl sung Histomount f r DAB Cat No 00 8030 GVA f r AEC oder Lichtstark Rot Cat Nr 00 8000 oder Clearmount f r AEC DAB oder Lichtstark Rot Cat Nr 00 8010 www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PI 08 4205 Rev 08 08 DCC 08 1089 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses PROBEN PRAPARATION 1 Gewebe in 10 igem neutral gepufferten Formalin fixiert oder anderen Fixierm
4. El anticuerpo prediluido V Line de Invitrogen est listo para usar con los sistemas de tinci n inmunohistoqu mica Ventana ES NexES y BenchMark Para obtener detalles espec ficos sobre el funcionamiento y la secuencia de procedimientos de los sistemas de tinci n inmunohistoquimica Ventana EST NexES y BenchMark consulte los manuales del usuario correspondientes y respectivos www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PI 08 4205 Rev 08 08 DCC 08 1089 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses CONTRATINCION Y CONSERVACION Protocolo Tiempo Contratinci n con hematoxilina 1 2 minutos Lavar en agua corriente con varios cambios hasta que el ba o de agua permanezca limpio Colocar en soluci n azulante es decir 10 mM PBS pH 7 4 con 2 cambios 15 20 segundos Lavar en agua destilada con 2 cambios 3 minutos en total Deshidratar secuencialmente en EtOH 70 95 95 100 y 100 Luego en xileno y xileno no deshidratar al usar AEC 2 minutos cada uno Aplicar cubreobjetos con soluci n de montaje Histomount Usar soluci n de montaje GVA cuando se use AEC
5. Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses CONTRECOLORATION ET CONSERVATION Protocole Dur e Contremarquer l h matoxyline 1 2 minutes Laver plusieurs reprises avec de l eau du robinet jusqu ce l eau soit bien claire Placer dans une solution d azurage par exemple PBS 10 mM pH 7 4 deux reprises 15 20 secondes Laver dans de l eau distill e deux reprises 3 minutes en tout D shydrater ensuite dans de l thanol 70 95 95 100 et 100 Ensuite dans du xyl ne et xyl ne Ne pas d shydrater lorsque l AEC est utilis 2 minutes chacun Appliquer le couvre lames avec une solution de fixation Histomount utiliser la solution de fixation GVA lorsque l AEC est utilis Laisser s cher avant de visualiser 10 minutes D PANNAGE Probl me Solution L immunocoloration du t moin Examiner d autres t moins positifs pr par s au cours de la m me analyse afin de d terminer si elle positif est faible est due l anticorps primaire ou un des r actifs communs secondaires V rifier que la lame comprend la bonne tiquette de code barres de Ventana Si elle est bien Le t moin positif est
6. www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses PREPARAZIONE DEI CAMPIONI 1 Utilizzare regolarmente tessuto fissato in 10 formalina neutra tamponata oppure in altro fissativo o in alternativa sezioni di tessuto congelato 2 Tagliare sezioni da 3 4 um e collocarle su vetrini caricati positivamente 3 Lasciare asciugare durante la notte a 37 C oppure per 2 4 ore a 58 C PRETRATTAMENTO Heat Induced Epitope Retrieval HIER Recupero di epitopi mediante calore se richiesto l Za 3 4 5 6 Dig 1 Deparaffinizzare i vetrini Le sezioni dei tessuti dovrebbero essere montate su vetrini rivestiti di silano poly L Lysine oppure HistoGrip Cat n 00 8050 Lavare i vetrini con acqua distillata per 3 per 2 min ciascuno Collocare un beker di vetro Pyrex da 1L contenente 500 mL di 0 01 M tampone citrato Cat n 00 5000 o soluzione EDTA Cat No 00 5500 su una piastra scaldante Riscaldare la soluzione tampone sino a quando bolle Questo passaggio pu essere preparato prima della deparaffinizzazione del vetrino poich il tampone pu richiedere diversi minuti per raggiungere il punto di ebollizione Collocare i vetrini in un rack scorrevole e metterli nel beker con tampone bollente Far bollire per 15 minuti Dopo il riscaldamento togliere il beker dalla piastra scaldante e far raffreddare per almeno15 20 minuti a temperatura ambien
7. ARIO Invitrogen recomienda el siguiente procedimiento para llenar los dispensadores de reactivo Ventana con el prediluido V Line de Invitrogen Consulte el Manual del Usuario de Ventana para obtener m s informaci n 1 Retire el tap n del extremo de la Jeringa de transferencia Ventana luego retire el mbolo por completo del cilindro y vuelva a colocar el tap n en el cilindro de la jeringa 2 Coloque una etiqueta a la Jeringa de reactivo y transferencia Ventana indicando el anticuerpo Invitrogen utilizado Vierta la ampolla que se adjunta de prediluido V Line 10 mL anticuerpo listo para usar en el cilindro de la Jeringa de transferencia Ventana luego vuelva a colocar el mbolo en el cilindro Invierta la Jeringa de transferencia Ventana colocando el tap n hacia arriba y el mbolo hacia abajo Luego retire el tap n Haga salir el aire de la Jeringa de transferencia Ventana empujando cuidadosamente el mbolo hacia el tap n Ahora retire el tap n de la punta de la Jeringa de reactivo Ventana Conecte la boquilla de la Jeringa de reactivo Ventana a la boquilla de la Jeringa de transferencia Ventana Dispense anticuerpo de la Jeringa de transferencia Ventana hacia la Jeringa de reactivo Ventana La cantidad m xima que puede contener la Jeringa de reactivo Ventana es 12 5 mL lo cual es suficiente para unas 120 pruebas 9 Coloque la etiqueta Invitrogen adjunta en la Jeringa de reactivo Ventana a DR
8. JASE A SU DISTRIBUIDOR LOCAL Representante autorizado de IVDD 98 79 EC Invitrogen Ltd Inchinnan Business Park 3 Fountain Drive Paisley PA4 9RF UK www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PI 08 4205 Rev 08 08 DCC 08 1089 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses e invitrogen CE Souris anti EGFr Clone 31G7 Pour Utilisation de Diagnostiques In Vitro X 08 4205 10 mL Anticorps de pr dilution V Line pr t l emploi Optimis pour les syst mes de coloration immunohistochimique Ventana ES M NexES et BenchMark Y UTILISATION VOULUE Pour Utilisation de Diagnostiques In Vitro L interpr tation doit tre faite par un pathologiste qualifi dans le cadre des ant c dents cliniques du patient et d autres tests de diagnostics REACTIF FOURNI Souris anti c EGFr est purifi partir de fluide ascite de souris et dilu dans du Phosphate Tamponn Salin PBS pH 7 4 et 1 d albumine de s rum de bovin BSA avec 0 1 azide de sodium NaN comme pr servatif Immunog ne EGFr humain d riv de cellules A 431 Isotype IgG Clone 31G7 Contr le Positif Cellules canc reuses du sei
9. Ltd Inchinnan Business Park 3 Fountain Drive Paisley PA4 9RF UK www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PI 08 4205 Rev 08 08 DCC 08 1089 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses
10. a hot plate Heat the buffer solution until it boils This step may be prepared before slide deparaffinization as the buffer may take several minutes to boil 4 Put the slides in a slide rack and place in the beaker with boiling buffer Keep it boiling for 15 minutes 5 After heating remove beaker from the hot plate and allow it to cool down for at least 15 20 minutes at room temperature 6 Rinse slides with PBS Cat No 00 3000 and begin the immunostaining protocol Enzyme Digestion if required 1 See Table 1 for specific enzyme digestion requirement and incubation time LOADING THE USER DEFINED DISPENSER The following procedure is recommended by Invitrogen for filling the Ventana Reagent Dispensers with Invitrogen s V Line Predilute Please refer to the Ventana Operator s Manual for further clarification 1 Remove the plug at the end of the Ventana Transfer Syringe then remove the plunger completely from the barrel and put the plug back on the syringe barrel 2 Appropriately label the Ventana Reagent and Transfer Syringe with the Invitrogen antibody in use Pour the enclosed vial of V Line Predilute 10 mL ready to use antibody into the barrel of the Ventana Transfer Syringe then put the plunger back into the barrel Invert the Ventana Transfer Syringe by positioning the plug upwards and the plunger downward Then remove the plug Displace the air out of the Ventana Transfer Syringe by carefully pus
11. bung auf Andere positive Kontrollen die w hrend desselben Verfahrens pr pariert wurden pr fen um zu bestimmen ob die Ursache der prim re Antik rper oder eines der allgemeinen sekund ren Reagenzien ist Positive Kontrolle ist negativ Sicherstellen dass der Objekttr ger mit dem richtigen Ventana Strichcode Etikett gekennzeichnet ist Bei richtiger Etikettierung m ssen andere positive Kontrollen die w hrend desselben Verfahrens pr pariert wurden gepr ft werden um zu bestimmen ob die Ursache der prim re Antik rper oder eines der allgemeinen sekund ren Reagenzien ist WARENZEICHEN Cap Plus Clearmount Digest All HistoGrip Histomount und Histostain NBA PicTure und Zymed sind Warenzeichen der Zymed Laboratories Inc Ventana ES NexES und BenchMark sind Marken der Ventana Medical Systems Inc V Line Antik rper werden ausschlieBlich von Zymed Laboratories Inc entwickelt und wurden nicht durch Ventana Medical Systems Inc genehmigt oder gef rdert Zymed und Ventana sind in keiner Weise angegliedert aneinander angeschlossen bzw miteinander verbunden Fiir genauere Information beziehen Sie sich bitte entweder auf die englische Version auf der Datenplatte oder auf www invitrogen com SOLLTEN SIE IRGENDWELCHE FRAGEN UBER DIESES PRODUKT HABEN WENDEN SIE SICH BITTE AN IHREN ORTLICHEN VERTEILER Bevollm chtigter Repr sentant f r IVDD 98 79 EC Invitrogen
12. e invitrogen CE Mouse anti Epidermal Growth Factor Receptor EGFr Clone 31G7 For In Vitro Diagnostic Use Lot No X 08 4205 10 mL V Line Predilute Ready to Use Antibody Optimized For Ventana ES NexES and BenchMark Immunohistochemistry Staining Systems INTENDED USE For In Vitro Diagnostic Use Invitrogen s monoclonal Mouse anti Epidermal Growth Factor Receptor EGFr antibody is intended to qualitatively stain squamous cell carcinoma in frozen and formalin fixed paraffin embedded tissue sections Interpretation must be made within the context of the patient s clinical history and other diagnostic tests by a qualified pathologist BACKGROUND The epidermal growth factor receptor EGFr HER 1 c erbB 1 is a 170 kDa transmembrane protein consisting of an extracellular EGF binding domain a short transmembrane region and an intracellular domain with ligand activated tyrosine kinase activity Two ligands can activate EGFr epidermal growth factor EGF and transforming growth factor alpha TGF a When TGF a binds to EGFr receptor tyrosine kinase activity is upregulated producing phosphorylation and an increase in cytosolic calcium within target cells This triggers increased synthesis of DNA and the proliferation and differentiation of target cells TGF a is thus an important effector of malignant transformation in a variety of cells EGFr is expressed in many normal epithelial tissues particularly in the basal layers
13. ges until water bath remains clear Place in blueing solution i e 10 mM PBS pH 7 4 with 2 changes 15 20 Seconds Wash in distilled water with 2 changes 3 Minutes Total i i 0 0 0 0 0 1 Dehydrate sequentially in 70 95 95 100 and 100 EtOH Then in xylene and xylene Do not dehydrate 2 Minutes Each when using AEC Apply coverslip with Histomount Mounting Solution Use GVA Mounting Solution when using AEC Allow to dry before viewing 10 Minutes TROUBLESHOOTING Problem Solution Positive control shows weak Check other positive controls prepared during the same instrument run to determine if it is due to the immunostaining primary antibody or to one of the common secondary reagents Assure that the slide has proper Ventana Bar Coding Label If it is labeled properly check other Positive control is negative positive controls prepared during the same instrument run to determine if it is due to the primary antibody or one of the common secondary reagents REFERENCES Cohen S et al J Biol Chem 257 1523 1539 1982 Chen WS et al Cell 59 33 43 1989 Pusztai L et al J Pathol 169 9 14 1993 Ozane B Richardson CS J Pathol 149 191 201 1986 Gusterson B et al Cell Biol Int Rep 8 649 658 1984 Damjanov I et al Lab Invest 55 5 588 592 1986 Niikura H et al Human Pathol 27 3 282 289 1996 Rusch V et al Ann Thorac Surg 62 798 810 1996 9 Agosti RM et al Virchows Arch Pathol Anat 420 321 325 1992 10 Ch
14. hing the plunger towards the plug Now remove the plug from the Ventana Reagent Syringe tip Attach the Ventana Reagent Syringe nozzle to the Ventana Transfer Syringe nozzle Dispense antibody from the Ventana Transfer Syringe into the Ventana Reagent Syringe The maximum amount held by the Ventana Reagent Syringe is 12 5 mL which provides approximately 120 tests 9 Place the enclosed Invitrogen label onto the Ventana Reagent Syringe Ww COS Invitrogen s V Line Predilute antibody is ready for use with the Ventana ES NexES and BenchMark Immunohistochemistry Staining Systems For specific details on operation and sequence of events of the Ventana ESTM NexES and BenchMark Immunohistochemistry Staining Systems refer to the respective and appropriate Ventana Operator s Manual www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PI 08 4205 Rev 08 08 DCC 08 1089 O 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses COUNTERSTAINING amp PRESERVATION Protocol Time Counterstain with hematoxylin 1 2 Minutes Wash in tap water with several chan
15. ica Ventana ES NexES y BenchMark PROPOSITO DE USO Para utilizaci n en diagn stico in vitro La interpretaci n de los resultados debe realizarla un pat logo cualificado dentro del contexto de la historia cl nica del paciente y otras pruebas diagn sticas REACTIVO SUMINISTRADO El anticuerpo EGFr de rat n se purifica a partir de fluido de ascitis en rat n y se diluye en tamp n fosfato PBS Ph 7 4 y seroalb mina bovina al 1 BSA con azida de sodio al 0 1 NaN como conservante Inmunog n EGFr humano derivado de las c lulas A 431 Isotipo IgG Clon 31G7 Control positivo Carcinoma de mama Pauta de tinci n esperada membrana celular y citoplasma ALMACENAMIENTO 2 8 C REQUISITOS PARA LA TINCI N INMUNOHISTOQU MICA El prediluido V Line de Invitrogen fue creado para usarse con los sistemas de tinci n inmunohistoqu mica Ventana ES NexES y BenchMark juntamente con los Equipos de detecci n Ventana y los dispensadores de reactivo Ventana Invitrogen ha realizado la valoraci n y controlado la calidad de este anticuerpo para garantizar un rendimiento uniforme y fiable La Tabla 1 resume los requisitos para la inmunotinci n Tabla 1 Requisitos para Tejido FFPE Especificaci n Eliminaci n de Epitope Inducida por Calor HIER No Necesaria Digesti n enzim tica Protease I Incubaci n enzim tica 8 minutos Incubaci n del anticuerpo primario 32 minutos
16. itteln auf regelm siger Basis benutzen oder gefrorene Gewebesetionen 2 3 4 um Sektionen schneiden und sie auf positiv ges ttigte Objekttrager legen 3 Uber Nacht bei 37 C trocknen oder fiir 2 4 Std bei 58 C VORBEHANDLUNG Hitze Induzierte Epitop Wiedergewinnung HIER wenn erforderlich 1 Objekttr ger deparaffinisieren Gewebesektionen sollten auf Silane Poly L Lysine oder HistoGrip pr pariert werden Cat Nr 00 8050 beschichtete Objekttrager 2 Objekttr ger mit destilliertem Wasser f r jeweils 2 Min waschen 3 Ein 1L Becherglas Pyrex das 500 mL von 0 01 M Citrat Puffer Cat Nr 00 5000 oder EDTA L sung Cat Nr 00 5500 enth lt auf eine hei e Platte legen Die Pufferl sung erhitzen bis sie kocht Dieser Schritt kann vor der Objekttr ger Deparaffinisierung vorbereitet werden da der Puffer mehrere Minuten braucht bevor er kocht 4 Die Objekttr ger in ein Objekttr gergestell setzen und das Becherglas mit dem kochenden Puffer plazieren Es 15 Minuten kochen lassen 5 Nach der Erhitzung das Becherglas von der heissen Platte entfernen und es fiir mindestens 15 20 Minuten bei Zimmertemperatur abk hlen lassen 6 Die Objekttr ger mit PBS Cat Nr 00 3000 sp len und das Immunof rbungs Protokoll beginnen Enzym Verdauung wenn ben tigt 1 Spezifische Enzym Digestionanforderungen und Inkubationszeiten sind Tabelle 1 zu entnehmen F LLEN DES VOM BENUTZER DEFINIERTEN DISPENSERS Invitrogen empfiehl
17. n Mod le de Coloration Attendue Membrane cellulaire et cytoplasmique STOCKAGE 2 8 C MAT RIEL N CESSAIRE POUR LA COLORATION IMMUNOHISTOCHIMIQUE La pr dilution V Line de Invitrogen a t con ue pour tre utilis e avec les syst mes de coloration immunohistochimique Ventana ES NexES et BenchMark en association avec les kits de d tection Ventana et les distributeurs de r actif Ventana Invitrogen a titr et v rifi la qualit de cet anticorps afin de garantir une performance homog ne et fiable Le tableau 1 regroupe le mat riel n cessaire pour l immunocoloration Tableau 1 Mat riel N cessaire pour Tissu FFPE Sp cification Recherche d Epitope de Chaleur par Induction HIER Pas Requis Enzyme de digestion Protease I Enzyme d Incubation 8 minutes Incubation des anticorps primaires 32 minutes MATERIELS NECESSAIRES MAIS PAS FOURNIS R actif Catalogue No 1 HistoGrip M 00 8050 2 PBS 0 01 M PBS 00 3000 3 Mayer H matoxyline 00 8011 4 Tampon citrate pH 6 0 si la RAC l exige 00 5000 5 Solution EDTA si la RAC l exige 00 5500 6 Syst mes de coloration immunohistochimique Ventana ES NexES ou BenchMark e Etiquettes code barres e Marqueur de lames automatis EST NexES ou BenchMark e Kit de d tection DAB ou AEC e Logiciel sp cifique au kit de d tection e Solution de lavage e R actif de t moin n gatif e Couvre lames liquide 7 Sol
18. n gatif tiquet e v rifier d autres t moins positifs pr par s au cours de la m me analyse afin de d terminer si c est d l anticorps primaire ou un des r actifs communs secondaires MARQUE DE FABRIQUE Cap Plus Clearmount Digest AITM HistoGrip Histomount et Histostain NBATM PicTure et Zymed sont des marques des Zymed Laboratories Inc Ventana ES NexES et BenchMark sont des marques d pos es de Ventana Medical Systems Inc Les anticorps V Line sont d velopp s exclusivement par Zymed Laboratories Inc et n insinuent pas une approbation ou un soutien de la part de Ventana Medical Systems Inc Zymed et Ventana ne sont en aucune fagon affili es ou associ es Pour des informations plus d taill es veuillez vous r f rer aux feuilles de donn es en version anglaise ou aller sur www invitrogen com SI VOUS AVEZ DES QUESTIONS SUR CE PRODUIT CONTACTEZ VOTRE DISTRIBUTEUR LOCAL Repr sentant Autoris pour IVDD 98 79 EC Invitrogen Ltd Inchinnan Business Park 3 Fountain Drive Paisley PA4 9RF UK www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PI 08 4205 Rev 08 08 DCC 08 1089 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By use of
19. n de tampon jusqu bullition Cette tape doit tre pr par e ant rieurement au d paraffinage des lames car porter le tampon bullition peut prendre plusieurs minutes Mettre les lames sur une grille lames et placer les dans le b cher avec le tampon en bullition Laisser bouillir pendant 15 minutes Apr s l chauffement retirer le b cher de la plaque chauffante et laisser le refroidir pour au moins 15 20 minutes temp rature ambiante Rincer les lames dans du PBS Cat No 00 3000 et commencer le protocole d immuno coloration yme de digestion si n cessaire Voir le tableau 1 pour la condition de digestion enzymatique sp cifique et le temps d incubation CHARGEMENT DU DISTRIBUTEUR TEL QUE D FINI PAR L UTILISATEUR Invitrogen recommande l utilisation de la proc dure ci apr s pour remplir les distributeurs de r actif Ventana avec la pr dilution V Line de Invitrogen Se reporter au manuel de l utilisateur de Ventana pour des d tails suppl mentaires 1 2 3 RAN 9 Retirer le bouchon l extr mit de la seringue de transfert Ventana retirer ensuite compl tement le piston du corps de pompe et remettre le bouchon dans le corps de pompe de la seringue Marquer de fa on appropri le r actif Ventana et la seringue de transfert avec l anticorps de Invitrogen utilis Verser le flacon de pr dilution V Line inclus 10 mL anticorps pr t l emploi dans le corps de pom
20. o Histomount Se si usa 1 AEC utilizzare la Soluzione di fissaggio GVA Lasciare asciugare prima dell osservazione 10 minuti www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PI 08 4205 Rev 08 08 DCC 08 1089 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses RISOLUZIONE DEI PROBLEMI Problema Soluzione Il controllo positivo mostra una Verificare altri controlli positivi preparati durante lo stesso ciclo di azionamento del dispositivo per immunocolorazione debole stabilire se ci causato dall anticorpo primario o da uno dei reagenti secondari comuni Accertarsi che il vetrino abbia l etichetta con il codice a barre Ventana corretta Qualora sia etichettato correttamente controllare altri controlli positivi preparati durante lo stesso ciclo di azionamento del dispositivo per stabilire se ci causato dall anticorpo primario o da uno dei reagenti secondari comuni Il controllo positivo negativo MARCHI REGISTRATI g i Cap Plus Clearmount Digest AIITM HistoGrip Histomount e Histostain NBA PicTure e Zymed sono marchi registrati di Z
21. of stratified epithelium and in squamous epithelium In normal tissues Invitrogen s monoclonal anti EGFr clone 31G7 stains epidermal cells of skin esophagus kidney testis placenta and prostate Positive staining appears as a linear to finely granular pattern in the cell membrane and adjacent cytoplasm or as coarsely granular cytoplasmic staining Invitrogen s 31G7 antibody reacts with EGFr and does not recognize the highly homologous c erbB 2 HER 2 protein Western blotting analysis with an EGFr vIll transfected NIH 3T3 cell line confirmed that clone 31G7 recognizes the 145 kDa variant III form of EGFr in addition to recognizing the wild type form of EGFr Over expression of EGFr has been observed in many types of neoplasia as the result of gene amplification and or increased protein transcription EGFr over expression has been detected in endometrial carcinoma correlated with myometrial invasion in lung neoplasms including metaplastic squamous epithelium squamous cell carcinoma adenocarcinoma and neuroendocrine lung tumors and in glioblastoma multiforme brain tumors EGFr expression has also been reported in most head and neck carcinomas Variable EGFr expression has been positively correlated with increasing tumor stage in transitional bladder renal cell and gastric carcinomas and in inverse correlation to estrogen receptor ER negative tumor status in breast carcinoma REAGENT PROVIDED Mouse anti EGFr antibod
22. os portaobjetos cargados positivamente 3 Secar durante toda la noche a 37 C o de 2 a 4 horas a 58 C PRETRATAMIENTO Eliminaci n de Ep tope Inducida por Calor HIER en caso necesario 1 Proceder con la desparafinaci n de las muestras Los cortes de tejido deber an montarse sobre portaobjetos cubiertos de silano poli L lisina o HistoGrip n de cat 00 8050 2 Lavar los portaobjetos con agua destilada 3 veces durante 2 minutos cada vez 3 Poner un vaso de precipitados Pyrex de 1 litro con 500 mL de tamp n de citrato 0 01 M n de cat 00 5000 o soluci n de EDTA n de cat 00 5500 en una placa de calor Calentar la soluci n tamp n hasta que hierva Este paso se puede preparar antes de la desparafinaci n de los portaobjetos puesto que pueden pasar varios minutos antes de que hierva el tamp n 4 Colocar los portaobjetos en una rejilla de soporte para portaobjetos y ponerlos en el vaso de precipitados con el tamp n hirviendo D jelo hervir durante 15 minutos 5 Despu s de calentar retire el vaso de precipitado de la placa de calor y d jelo que se enfr e a temperatura ambiente durante al menos 15 6 20 minutos 6 Enjuagar los portaobjetos con PBS n de cat 00 3000 y proceda con el protocolo de inmunotinci n Digesti n enzim tica en caso necesario 1 Consulte la Tabla 1 para ver el requisito espec fico de digesti n enzim tica y el tiempo de incubaci n CARGA DEL DISPENSADOR DEFINIDO POR EL USU
23. pe de la seringue de transfert de Ventana ensuite remettre le piston dans le corps de pompe Retourner la seringue de transfert de Ventana le bouchon tourn vers le haut et le piston vers le bas Retirer ensuite le bouchon Chasser l air de la seringue de transfert Ventana en poussant doucement le piston vers le bouchon Retirer ensuite le bouchon de l extr mit de la seringue de r actif Ventana Attacher l embout de la seringue de r actif Ventana sur l embout de la seringue de transfert Ventana Faire passer l anticorps de la seringue de transfert Ventana dans la seringue de r actif Ventana La quantit maximale contenue dans la seringue de r actif Ventana est de 12 5 mL ce qui permet d effectuer environ 120 tests Poser l tiquette de Invitrogen ci jointe sur la seringue de r actif de Ventana L anticorps de pr dilution V Line de Invitrogen est pr t tre utilis avec les syst mes de coloration immunohistochimique de Ventana ES NexES et BenchMark Pour des d tails sp cifiques concernant le fonctionnement et la s quence des v nements des syst mes de coloration immunohistochimique de Ventana EST NexES et BenchMark se reporter au manuel de l utilisateur Ventana en question www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PI 08 4205 Rev 08 08 DCC 08 1089 2007 Invitrogen
24. riassuntivamente i requisiti per l immunocolorazione Tabella 1 Requisiti per Tessuto FFPE Specificazione Recupero di epitopi mediante calore HIER Non Richiesto Digestione enzimatica Protease I Incubazione enzimatica 8 minuti Incubazione dell anticorpo primario 32 minuti MATERIALI NECESSARI MA NON FORNITI Reagente Catalogo n 1 HistoGrip M 00 8050 2 PBS 0 01 M PBS 00 3000 3 Ematossilina di Mayer 00 8011 4 Soluzione tamponata a base di citrato pH 6 0 se necessaria per il HIER 00 5000 5 Soluzione a base di EDTA se necessaria per il HIER 00 5500 6 Sistemi di colorazione immunoistochimica Ventana ES NexES e BenchMark e Etichette con codice a barre e Coloratori automatici di vetrini EST NexES o BenchMark e Kitdirilevazione DAB o AEC e Software specifico per il kit di rilevazione e Soluzione di lavaggio e Reagente controllo negativo e Coprivetrino liquido 7 Soluzione montante Histomount per DAB Cat n 00 8030 GVA per AEC oppure rosso solido Cat n 00 8000 oppure Clearmount per AEC DAB oppure rosso solido Cat n 00 8010 www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PI 08 4205 Rev 08 08 DCC 08 1089 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or
25. ringa di trasferimento Ventana spingendo cautamente lo stantuffo verso il tappo A questo punto rimuovere il tappo dalla punta della siringa di trasferimento Ventana Collegare l ugello della siringa per reagenti Ventana all ugello della siringa di trasferimento Ventana Erogare l anticorpo dalla siringa di trasferimento Ventana alla siringa per reagenti Ventana La siringa per reagenti Ventana ha una capacit massima di 12 5 mL ovvero un quantitativo sufficiente per eseguire 120 prove circa Attaccare l etichetta acclusa Invitrogen sulla siringa per reagenti Ventana Ventana ESM NexES e BenchMark Per informazioni particolareggiate sul funzionamento e la sequenza procedurale dei sistemi di colorazione immunoistochimica Ventana ES NexES e BenchMark si prega di consultare i rispettivi Manuali per l utente Ventana CONTROCOLORAZIONE E CONSERVAZIONE Protocollo Tempo Controcolorare con Ematossilina 1 2 minuti Lavare in acqua di rubinetto ricambiata diverse volte finch il bagnomaria rimane limpido Immergere in soluzione azzurrante ovvero 10 mM di PBS pH 7 4 eseguendo 2 ricambi 15 20 secondi Lavare in acqua distillata eseguendo 2 ricambi 3 minuti in tutto disidrata Disidratare sequenzialmente in EtOH al 70 95 95 100 e 100 Quindi in xilene e xilene Non a 2 minuti ciascuno re se si usa AEC Applicare il coprivetrino con la Soluzione di fissaggi
26. ristensen ME Danish Med Bull 45 2 121 134 1998 11 Neal DE et al Lancet 1 366 368 1985 12 Toi M et al Int J Cancer 43 220 225 1989 e ON On Be Ga bo TRADEMARKS Cap Plus Clearmount Digest All HistoGrip Histomount and Histostain NBA PicTure and Zymed are trademarks of Zymed Laboratories Inc Ventana ESTM NexES and BenchMark are trademarks of Ventana Medical Systems Inc V Line antibodies are developed solely by Zymed Laboratories Inc and do not imply approval or endorsement by Ventana Medical Systems Inc Zymed and Ventana are not affiliated associated or related in any way Authorized Representative for VDD 98 79 EC Invitrogen Ltd Inchinnan Business Park 3 Fountain Drive Paisley PA4 9RF UK www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PI 08 4205 Rev 08 08 DCC 08 1089 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses invitrogen ce Anticuerpo EGFr de rat n Clon 31G7 Para utilizaci n en diagn stico in vitro X 08 4205 10 mL Anticuerpo prediluido V Line listo para usar Optimizado para los sistemas de tinci n inmunohistoquim
27. t die folgende Methode zum F llen der Ventana Reagenz Dispenser mit Invitrogen V Line Vorverd nnung Weitere Anweisungen sind dem Ventana Benutzerhandbuch zu entnehmen 1 DOO 9 Den Verschluss am Ende der Ventana Transferspritze entfernen dann den Kolben ganz aus dem Zylinder herausziehen und den Verschluss wieder auf dem Spritzenzylinder anbringen Die Ventana Reagenz und Transferspritze entsprechend mit dem verwendeten Invitrogen Antik rper kennzeichnen Das beiliegende Fl schchen mit V Line Vorverd nnung 10 mL gebrauchsfertiger Antik rper in den Zylinder der Ventana Transferspritze geben und dann den Kolben wieder in den Zylinder schieben Die Ventana Transferspritze invertieren Dazu den Verschluss nach oben und den Kolben nach unten richten Dann den Verschluss entfernen Zum Entl ften der Ventana Transferspritze den Kolben vorsichtig in Richtung Verschluss schieben Dann den Verschluss von der Spitze der Ventana Reagenzspritze abnehmen Die Ventana Reagenzspritzend se an der Ventana Transferspritzend se befestigen Antik rper aus der Ventana Transferspritze in die Ventana Reagenzspritze spritzen Das maximale Fassungsverm gen der Ventana Reagenzspritze betr gt 12 5 mL und reicht f r etwa 120 Tests Das beiliegende Invitrogen Etikett auf der Ventana Reagenzspritze anbringen Der Invitrogen V Line Vorverd nnungs Antik rper kann jetzt mit den immunhistochemischen F rbungssystemen Ven
28. tana EST NexES und BenchMark eingesetzt werden Spezifische Details zum Betrieb und zur Ereignissequenz der immunhistochemischen F rbungssysteme Ventana ES NexES und BenchMark sind den entsprechenden Ventana Benutzerhandb chern zu entnehmen GEGENFARBUNG UND KONSERVIERUNG Protokoll Zeit Gegenf rbung mit H matoxylin 1 2 Minuten Mehrmals in Leitungswasser waschen bis das Wasserbad sauber bleibt Zweimal in Blaul sung platzieren z B 10 mM PBS pH 7 4 15 20 Sekunden Zweimal in destilliertem Wasser waschen Inggesamt 3 Minuten Nacheina Bei Verwendung von AEC nicht dehydrieren nder in 70 95 95 100 und 100 EtOH dehydrieren Dann in Xylen und Xylen dehydrieren Je 2 Minuten Deckglas mit Histomount Fixativ anbringen f r AEC muss GVA Fixativ verwendet werden Vor der Betrachtung trocknen lassen 10 Minuten PI 08 4205 www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com Rev 08 08 DCC 08 1089 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses FEHLERBEHEBUNG Problem L sung Positive Kontrolle weist eine schwache Immunf r
29. te Risciacquare i vetrini con PBS Cat n 00 3000 ed iniziare il protocollo di immunocolorazione estione Enzimatica se richiesto Per il tempo di incubazione ed i requisiti specifici per la digestione enzimatica consultare la Tabella 1 CARICAMENTO DELL EROGATORE DEFINITO DALL UTENTE Invitroge n raccomanda di osservare la seguente procedura per il riempimento degli erogatori di reagente Ventana con l anticorpo prediluito V Line di Invitrogen Per ulteriori chiarimenti si prega di consultare il Manuale per l utente Ventana 1 REN 9 L anticorpo prediluito V Line di Invitrogen fornito pronto per l uso ed da utilizzarsi con i sistemi di colorazione immunoistochimica Rimuovere il tappo posto sull estremit della siringa di trasferimento Ventana quindi estrarre completamente lo stantuffodal cilindro e rimettere il tappo sul cilindro della siringa Etichettare debitamente la siringa per reagenti e la siringa di trasferimento Ventana specificando il nome dell anticorpo Invitrogen utilizzato Versare il contenuto della boccetta acclusa di anticorpo prediluito V Line di Invitrogen 10 mL anticorpo pronto per l uso nel cilindro della siringa di trasferimento Ventana quindi reinserire lo stantuffo nel cilindro Capovolgere la siringa di trasferimento Ventana in modo che il tappo sia rivolto verso l alto e lo stantuffo verso il basso Quindi rimuovere il tappo Eliminare l aria dalla si
30. these products you accept the terms and conditions of all applicable Limited Use Label Licenses e invitrogen CE Topo anti EGFr Clone 31G7 Per uso diagnostico In Vitro X 08 4205 10 mL Anticorpo prediluito V Line Pronto per l uso Ottimizzato per i sistemi di colorazione immunoistochimica Ventana ES NexES e BenchMark SCOPI D UTILIZZO Per uso diagnostico In Vitro L interpretazione deve essere effettuata da un patologo qualificato entro il contesto dell anamnesi clinica del paziente ed in considerazione di altri test diagnostici REAGENTI FORNITI Topo anti EGFr viene purificato da liquido ascitico di topo e diluito in soluzione salina tamponata al fosfato PBS pH 7 4 e 1 di albumina di siero bovino BSA con 0 1 sodio azide NaN come conservante Immunogeno EGFr umano derivato dalle cellule A 431 Isotipo IgG Clone 31G7 Controllo positivo Carcinoma mammario Modello di colorazione previsto Membrana cellulare e citoplasmatica CONSERVAZIONE 2 8 C REQUISITI PER LA COLORAZIONE IMMUNOISTOCHIMICA L Anticorpo diluito V Line di Invitrogen stato sviluppato per l uso con i sistemi di colorazione immunoistochimica Ventana ES NexES e BenchMark insieme ai kit di rilevazione Ventana e gli erogatori di reagente Ventana Invitrogen ha titolato questo anticorpo e lo ha sottoposto ad un controllo della qualit per garantirne la performance coerente ed affidabile La Tabella 1 elenca
31. use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses MATERIALS REQUIRED BUT NOT PROVIDED Reagent Catalog No 1 HistoGrip M 00 8050 2 Antibody Diluent 00 3118 3 PBS 0 01 M PBS 00 3000 4 Citrate Buffer pH 6 0 if required for HIER 00 5000 5 EDTA Solution if required for HIER 00 5500 6 Ventana ESTM NexES or BenchMark Immunohistochemistry Staining Systems e Bar Code Labels e ES NexES or BenchMark Automated Slide Stainer e DAB or AEC Detection Kit e Detection Kit specific software e Wash Solution e Negative Control Reagent e Liquid Coverslip 7 Mounting solution Histomount for DAB Cat No 00 8030 GVA for AEC or Fast Red Cat No 00 8000 or Clearmount for AEC DAB or Fast Red Cat No 00 8010 SPECIMEN PREPARATION 1 Use tissue fixed in 10 neutral buffered formalin or other fixative on regular basis or frozen tissue sections 2 Cut 3 4 um sections and place on positively charged slides 3 Dry overnight at 37 C or for 2 4 hrs at 58 C PRETREATMENT Heat Induced Epitope Retrieval HIER if required 1 Deparaffinize slides Tissue sections should be mounted on silane poly L Lysine or HistoGrip Cat No 00 8050 coated slides 2 Wash slides with distilled water 3 times for 2 min each 3 Place a 1L glass Pyrex beaker containing 500 mL of 0 01 M citrate buffer Cat No 00 5000 or EDTA solution Cat No 00 5500 on
32. ution de Monture Histomount pour DAB Cat No 00 8030 GVA pour AEC ou Rouge Fixe Cat No 00 8000 ou Clearmount pour AEC DAB ou Rouge Fixe Cat No 00 8010 www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PI 08 4205 Rev 08 08 DCC 08 1089 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses PREPARATION DE SPECIMENS 1 Utiliser un tissu fix dans 10 Formaline Neutre Tamponn e ou autres fixatifs de base normale ou des sections gel es de tissu 2 Couper des sections 3 4 um et placer sur des lames charg es positivement 3 S cher toute la nuit 37 C ou pendant 2 4 hrs 58 C PRETRAITEMENT Recherche d Epitope de Chaleur par Induction HIER si n cessaire 1 2 3 6 Enz 1 D paraffiner les lames Les sections de tissu doivent tre mont es sur du silane du poly L Lysine ou du HistoGrip Cat No 00 8050 lames enduites Laver les lames dans 3 bains d eau distill e pendant 2 min chacun Placer un b cher en verre 1L Pyrex contenant 500 mL de 0 01 M tampon de citrate Cat No 00 5000 ou solution d EDTA Cat No 00 5500 sur une plaque chauffante Chauffer la solutio
33. www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PI 08 4205 Rev 08 08 DCC 08 1089 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses MATERIALES NECESARIOS PERO NO SUMINISTRADO Reactivo N de catalogo 1 HistoGrip M 00 8050 2 PBS PBS0 01 M 00 3000 3 Hematoxilina de Mayer 00 8011 4 Tampon citrato pH 6 0 si se necesita para HIER 00 5000 5 Soluci n EDTA si se necesita para HIER 00 5500 6 Sistemas de tinci n inmunohistoquimica Ventana EST NexES o BenchMark e Etiquetas de c digos de barras e Tinte de portaobjetos automatizado ES NexES o BenchMark e Equipo de detecci n DAB o AEC e Software espec fico para el Equipo de detecci n e Soluci n de lavado e Reactivo para control negativo e Cubreobjetos l quido 7 Soluci n de montaje Histomount para DAB n de cat 00 8030 GVA para AEC o para Fast Red n de cat 00 8000 o Clearmount para AEC DAB o Fast Red n de cat 00 8010 PREPARACI N DE LA MUESTRA 1 Utilizar tejido fijado en formalina neutra tamponada al 10 u otro fijador general o cortes de tejido congelados 2 Hacer cortes de 3 4 um y colocarlos sobre l
34. y clone 31G7 is purified from mouse ascites and diluted in phosphate buffered saline PBS pH 7 4 and 1 bovine serum albumin BSA with 0 1 sodium azide NaN3 as a preservative Immunogen Human EGFr derived from A 431 cells Total protein concentration g L Clone 31G7 Isotype IgG Antibody concentration mg L STORAGE 2 8 C POSITIVE CONTROL TISSUE Breast carcinoma EXPECTED STAINING PATTERN Cell membrane and cytoplasm REQUIREMENTS FOR IMMUNOHISTOCHEMISTRY STAINING Invitrogen s V Line Predilute was developed for use with the Ventana ES NexES and BenchMark Immunohistochemistry Staining Systems in combination with Ventana Detection Kits and Ventana Reagent Dispensers Invitrogen has titered and quality controlled this antibody to assure consistent and reliable performance Table 1 summarizes the requirements for immunostaining Table 1 Requirement for FFPE Tissues Specification HIER Heat Induced Epitope Retrieval Not Required Enzyme Digestion Protease I Enzyme Incubation 8 minutes Primary Antibody Incubation 32 minutes www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PI 08 4205 Rev 08 08 DCC 08 1089 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By
35. ymed Laboratories Inc Ventana ES NexES e BenchMark sono marchi commerciali di propriet di Ventana Medical Systems Inc Gli anticorpi V Line sono sviluppati esclusivamente da Zymed Laboratories Inc e non implicano alcuna autorizzazione o approvazione da parte di Ventana Medical Systems Inc Zymed e Ventana non sono imprese consociate affiliate o collegate in alcun altro modo Per informazioni pi dettagliate vi preghiamo di consultare la versione inglese del catalogo oppure visitate il sito www invitrogen com SE AVETE DOMANDE SUL PRODOTTO CONTATTATE IL VOSTRO DISTRIBUTORE PIU VICINO Rappresentante Autorizzato per IVDD 98 79 EC Invitrogen Ltd Inchinnan Business Park 3 Fountain Drive Paisley PA4 9RF UK www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PI 08 4205 Rev 08 08 DCC 08 1089 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses e invitrogen CE Maus Anti EGFr Klon 31G7 F r In Vitro Diagnostischen Gebrauch X 08 4205 10 mL V Line Vorverd nnung gebrauchsfertiger Antik rper Optimiert f r die immunhistochemischen F rbungssysteme Ventana ES NexES und BenchMark
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