ジーンキューブ MTB - 医薬品医療機器総合機構
Contents
1. 2015 9 30 7 2015 1 21 6 22200AMX00914000 87749 NY MTB GENECUBE SDS SDS2. 4 1 PCR
3. 1 KOD Mix KOD DNA dNTP MTB Mix MTB F MTB R MTB QProbe 3 4 5 dNTI M1 MT P F F QProbe IC Mix B PC TB NC DNA Polymerase Chain Reaction PCR 1 DNA Dnaj
4. 3 4 1 2 3 2 8
5. 1 4 1 4 4 10 GENECUBE 5 DnaJ DNA PCR 122 1 2 99 2 160 3 4 100 0 1 60 0 1 61 1
6. ET E 1 a c b 100rzL 100 d e 2 3 H
7. GENECUBE 6 1 1 2 GENECUBE 3 lt
8. 8 1 35 0 C 0 0 sec 94 0 C 30 0 sec 97 0 1 0 sec 58 0 C 3 0 sec 63 0 5 0 sec 35 0 C 0 0 sec 50 0 2 94 0 C 30 0 sec 39 0 C 30 0 sec 40 0 75 0 0 09 C sec 1 GENECUBE Invalid 1 Tnvalid 2 EF E 1 2 MTB PC MTB NC
9. 2 DNA 3 KOD Mix IC Mix MAC 4 1000 10 EDTA KOD Mix MTB Mix IC Mix
10. PCR DNA QProbe 1 1 DNA 2 1 3 3
11. 122 99 2 121 122 2 59 1 2 0 62 2 122 99 2 121 122 3 80 0 0 80 160 100 0 160 160 4 80 0 0 80 160 100 0 160 160 2 3 1 1 N95 5
12. T 2001 Mar 15 29 6 e34 4 Torimura M et g Fluorescence Quenching Phenomenon by Photoinduced Electron Transfer between a Fluorescent Dye and a Nucleotide Base Anal Sci 2001 Jan 17 1 155 160 5 2007 2007 31 36 TOYOBO ldeas amp Chemistry 530 8230 2 8 TEL 06 6348 3335 FAX 06 6348 3833 104 6004 8 10 03 6219 7608 1 2
13. 4 5 80 1 SAP NALC NaOH NALC NaOH 5 SAP 50mL 1mL 15 mL
14. 80C 10 20 3 3 GENECUBE S GENECUBES 3000 xg 4 C 20 NALC r 100
15. fT 1 4 Mycobacterium tuberculosis M bovis M atfricanum M microt DNA 38 DNA 10 GENECUBE
16. Y mn DNA H DNA 3 1 A5249K M 1 E 1 3 1 3 3 SO EO 2 i SO
17. 1 KOD Mix 140u LX6 MTB Mix 140u LX6 IC Mix MTB 300LX1 MTB PC 300 LX1 MTB NC 300 1 1 Yamada Noda M et g Mycobacterium species identification A new approach via 7a gene sequencing Syst Appl Microbiol 2007 Sep 30 6 453 462 2 Kitabayashi M et g Gene Cloning and Polymerase Chain Reaction with Proliferating Cell Nuclear Antigen from Thermococcus kodakaraensis KOD1 Biosci Biotechnol Biochem 2002 Oct 66 10 2194 2200 3 Kurata S et g Fluorescent quenching based quantitative detection of specific DNA RNA using a BODIPY R FL labeled probe or primer Nucleic Acids Res 10 24 lt NQ MTBGC PS150930
18. NALC NaOH 2 20 NALC NaOH 20 25 15 50mL pH6 8 1mL 20 1 2 NALC NaOH 4 50mL 0 1mol L 2 9 50mL 121C 15 0 5g 24 2
19. F R KOD D dNTP A 1 HH 1 Probe 3 4 H 1 DNA KOD MTB QProbe DNA 1 Q HH 1
20. KOD Mix MTB Mix 2 MTB PC MTB NC 0 5mL 38 3 3 IC KOD Mix 3 M Mix 1 B Mix 3 4 2335 7 5 GENECUBE 6 GENECUBE 2 510nm 550nm 573nm 613nm MTB PC 3 6 E MTB NC
21. 2 3 4 5 6 0 5 7 121 C 20 0 50 1
22. MTB PC MTB NC GENECUBE lt gt 8 T 028 0 5mL 72 699S GENECUBE 3 8 1
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