“WAKO BIO WINDOW” No.94(2009.02)
Contents
1. HRP a i No lsotype SE Es No 3 1 2 g 207 013 21851 Anti GST Monoclonal Antibody GST lgG 5A7 0 125 1zg m g 20p Beads slurry 2 3ug 20ul 010 21861 Anti 6X His Monoclonal Antibody lgG 9F2 0 125 lug mi lt 720 6XHis Beads slurry 1 2g 20z 017 21871 1 Anti c Myc M lonal Antibod lgG 9E10 0 125 1 TO EQKLISEEDL a dy EE EE 1 1 2 g 20 014 218811 Anti HA M lonal Antibod lgG 4B2 0 125 1 NN YPYDVPDYA 8 IN Anti GST M lonal Antibody 011 21891 1 Ni GST IgG 5A7 1 250 1 2000 Peroxidase Conjugated Anti c Myc M lonal Antibody EO NAR IgG 9E10 1 250 1 2000 Peroxidase Conjugated EQKLISEEDL Anti HA Monoclonal Antibody 011 21911 jgG 4B2 1 250 1 2000 NE Peroxid2. 1 FITC 7 14 FITC 2 FITC HITG gt BSS HBSS ese SOO SS SS RS SS ee eS 200m gt Pen Strep EERRRPPPPPPPPPPPPPPPPTPPHPPHPPRHRHPTTPTPTT 100z PBS meee 250m FITC TF BB 85ul gt PP 250m ete 43z gt
3. Tocris An YX 4 hae CT ro Or Or Mg 3089 3041 3077 3078 3079 H Lt Lt 1 i NHMe 2 T TT N MeHN MeHN N N HCI N TT 3017 2556 1985 2911 F CI NMe HCI 1 N CI 2993 HN Sr CI RS HCI 00 2HCI N MeO CN N S 3189 3188 3085 Ol 3s0gg Cjo33466 ER 519148 48 2 Omg 36 400 50mg 160 000 3041 CP809101 hydrochloride 5 HT 479683 64 2 oe Ts000 513 64021 5 HT 10mg 48 900 i LY 272015 hydrochl 2B 72895 19 7 5 MM NN EU FOO 2208000 510 64031
4. 9 9 3 C3G C3G c3G 24 well plate 1 2X10 well 3 C3G C3G 5 O C3G OH C3G
5. ELISA Wako ELISA S N Reagent A Reagent B 2 CCT ELISA x1 x2 x1 x2 SDS PAGE 6 ee 1 Reagent A
6. Transient Receptor Families GPCR OST00033W TRPM4 Melastatin 4 OSM00019A p75NTR http www osenses com index php http wako chem cOo jp SiyaKu jutaku index htm fC EH senses eadgqemnts FOr Every Ssenses OSG000012W GLUT 3 U YA FEB 2009 No 94 HF HF 7 Chu C C N6
7. LH TSH GH 0 313 10ng m 450nm 620nm 10 Well 5 3 50 8 2 CV 5 4 3 4 CV 5 60 000 60 000 631 10161 635 13741 AKRTS 010 TSH 96 AKRGH 010 GH 96 G KY FEB 2009 No 94 e http www siyaku com Hako joWWinodow A 3 O
8. NG6 17 4 1g mg KNO 2 830 FeSO 7H O 27 85 NH SO 463 MnSO 4FHLO 4 4 KH PO 400 H BO 1 6 MgSO 7H O 185 ZnSO 7H O 1 5 CaCL 2H O 166 KI 0 8 Na EDTA 37 25 1 1 4 1g 17 17 20C 15 107 1 41g 107 107 120C 15 Fe lt gt
9. 4 3 Buffer 250 SBS the putor weel hie Brolein wel No i 511 51991 80 000 5 192 AAM AxyGem Crystallography Plate No 511 51991 192 96 Al H12 SBS No 518 52001 CP AxyPear 192 100 AxyPearl Lid for High
10. IgG Fig 1 50xg Bio Rad Criterion gel Cdk1 34 kal PVDF 196 in TBST Cdk1 0 2g m Liqht Chain 22 kha HRP 0 16 g m Clean Blot IP Detection Reagent HRP 0 2ug ml NFkB Bax IP Fig 2 A B C D Clean Blot Clean Blot IP Detection Detection GAR HRP Reagent GAR HRP Reagent A C IP B D NFxB A549 A G NFxB Bax
11. 1 637 14661 http wako chem cO jD SIyaKu jutaku index htm Mini column Cellufine Sulfate 1m 1m X5 28 000 637 14781 20051 Mini column Cellufine ET clean L 1m 1m X5 28 000 634 14791 20151 Mini column Cellufine ET clean S 1m 1m X5 28 000 637 146801 20251 Mini column Cellufine PB 1m 1m X5 28 000 638 14571 19551 Mini column Cellufine Phosphate 1m 1m X5 28 000 633 14641 19805 51 Mini column Cellufine A 500 1m 1m X5 20 000 630 14771 19907 51 Mini column Cellufine Q 500 1m 1m X5 25 000 639 14621 19800 51 Mini column Cellufine C 500 1m 1m X5 20 000 632 14731 19900 51 Mini column Cellufine Phenyl 1m 1m X5 23 000 636 14751 19905 51 Mini column Cellufine Butyl 1m 1m X5 23 000 638 14711 19845 51 Mini column Cellufine Chelate 1m 1m X5 28 000 G OK FEB 2009 No 94 HF S Hako joWWindow Ago2 Wako Anti Mouse Aqgo2 Monoclonal Antibody Argonaute2 Ago2 RNAi
12. 8 400 C3G 1uM C3G 10uM C3G 100ugM T 4 306 15701 P1 135 000 ij ision 1 WI nr eat Glycogen Assay Kit a 3 ek OxiRed Probe 570nm Ex Em 535nm 587nm i a IRN NI 1 lt OPD JS DE 90 Hydrolysis Buffer 50ul Development Buffer 15m OxiRed PO vial i DMSO 0 4m Hydrolysis Enzyme Mix Hydrolysis B
13. Purification of AduckyHokkaido Wac 1 04 HSN1 2 IP4W 8 PCDFCOHTTCHOHCLTLCTT 100D 15 SO0O 5 wr SN TF A 05 2 Elution EN LL LL LL LAC Ne 0 gt Fraction ho HA Titer Protein ug La 1 55 Elutlon 5 1 BZ 51 0 01M Phosphate buffer pH 7 4 0 01M Phosphate buffer 0 19M NaCl pH 7 2 0 01M Phosphate buffer 3M NaCl pH 7 0 1 propiolactone HA Titer A B
14. el 2 1 i Choline Assay Buffer ee 25m a i Choline Probeeeeeeeeeeeeeeeeeeeeeeeeeeseeeeeeeeeeeeee vial E 1 4 DMSO RT 400 Choline Enzyme Mix ee 1 vil 0 7 Acetylcholinesterase PP vial Choline Standard 5umol vial 1 2 a 5 Chaline nmoliwell X Standard Curve Standard Curve Choline Assay Buffer Choline Standard Choline Assay Buffer Reaction Mix Choline Assay Buffer RBBeHIBh Mix NO Choline Enzyme Mix Choline Assay Buffer 1 Reaction Mix 30 OD 570nm Ex Em 535 590nm
15. A B C D DAPI No 518 61911 ARY010 Proteome Profiler Human Pluripotent Stem Cell Array Kit kit 104 000 http www rndsystems com U TN R amp D Systems TECHNE CORPORATION 4901382 http wako chem co jp siyaku jutaku index htm lt FEB 2009 No 94 w HF ee 7272 2 Thermo EFI Clean Blot IP Detection Reagent HRP IAP 0 gt 1gG
16. 1P IgG 2 Table 1 IgG Fig 1 Fig 2 Table 1 IgG ge A G lgG TP gC Buffer lgG lgGs 1gG4 BSA SuperBlock Blocking Buffer Starting Block Blocking Buffer Mouse IgG lgG lgGs _ Rat HRP AP HRP AP
17. 2 14g m 99 2 a 3 2g m 43 43 4 R 2g m 394 3 5 IlgG 4 2 54g m 5 6XHis 6 7 FEB 2009 No 94 e http www siyaku com ECL DAnti GST Monoclonal Antibody No 013 21851 14g m GST 100 10 10u7 Anti 6x His Monoclonal Antibody No 010 21861 0 2 g m 6XHis 1007 10z Anti c Myc Monoclonal Antibody No 017 21871 0 25 g m c Myc 10ng Anti HA Monoclonal Antibody No 014 21881
18. K615 100 Choline Acetylcholine Quantification Kit 517 37 431 514 38421 513 37411 Ho17 38411 513 36511 511 36431 518 36441 510 38521 K603 100 K264 100 K606 100 K337 100 K347 100 K639 100 K607 100 K608 100 K661 100 FEB 2009 No 94 Cholesterol Cholesteryl Ester Quantitation Kit Glutathione Assay Kit GSH GSSG Total Glucose Assay Kit NAD NADH Quantification Kit NADP NADPH Quantification Kit L Amino Acid Quantitation Kit Lactate Assay Kit Uric Acid Assay Kit Ascorbic Acid Assay Kit 100 Assay 100 Assay 100 Assay 100 Assay 100 Assay 100 Assay 100 Assay 100 Assay 54 000 73 000 46 000 65 000 65 000 56 000 61 000 61 000 56 000 No mn U T http www siyaku com 727oZ 27477 TOCRIS F 5 Hydroxytryptamine 5 HT NN MIR IGR INS 3 7 5 HT 5 HT
19. 5 CV 5 4 4 CV 5 634 14291 mn hh AKDF2 020 TAC DerfIL ELISA Kit 96 G KY FEB 2009 No 94 e http www siyaku com Hako joWWindow Trans Genic Inc S _LL me O GPCR Anti Mouse GPR40 Monoclonal Antibody Clone No G16 G G protein coupled receptor GPCR GPR40 GPR40 gm r H 3
20. 1 4 8 NGF BDNF 3 i 4 Neurotrophin 4 Human recombinant 140 000 rhNT 4 NT 4 5 Neurotrophic 4 5 sg 39900 sz _ Brain Derived Neurotrophic Factor Human recombinant 020 12913 BDNF 40 000 _ Nerve Growth Factor Human recombinant 141 07601 rh2 NGE 20g 39 000 2 5S Nerve Growth Factor from Mouse Submaxillary Grand a 143 04861 m2 5S NGE 10g 15 700 Neurotrophin 3 Human recombinant a 141 06643 rhNT 3 10 g 45 000 ELISA 6 Ni NalleIeM e e LH Luteinizing Hormone LH LH
21. NSAIDs CF3 CH F N 0 281 23 COX 1 IC 0 80uM COX 2 IC 210pAM Kakuta H et g Cyclooxygenase 1 selective inhibitors are attractive candidates for analgesics that do not cause gastric damage Design and in vitro in vivo evaluation of a benzamide type cyclooxygenase 1 selective inhibitor Med Chem 51 2400 2411 2008 TFAP AF 5 Amino 2 pyridinyl 4 trifluoromethyl benzamide http wako chem co jp siyaku jutaku index htm FEB 2009 No 94 205 17381 20 000 K SY Hako joWWindow 8 Wako L_ 4 3 4 TrkB
22. mRNA siRNA microRNA mRNA RISC RNA induced silencing complex UP Ago2 RNAi RNA microRNA siRNA 0 Ago2 RISC microRNA 0 05 Sodium Azide 10 Glycerol TBS pH7 4 Clone No 2D4 IgG Ago2 N 2 10 1mg m 1 P388D1 Ago2 IP IP SDS PAGE kDa 1 2 1 2 Lane 1 IgG IP a Lane
23. HPLC 8 OH dG HPLC 8 OH dG O O HN N HN N l 5 HzN NN HzN NN dR DNA dR dG B 0H dG DNA ce 8 OH dG HPLC OAI 0 13 0 08 83 OH dG EE 0 i 0 4 8 12 16 20 24 28 min 8 OH dG E 8 OH dG 12 000 sample 8 OH dG 20 000 sample 8 OH dG 8 OH Gua 30 000 sample DNA 8 OH dG i DNA 8 OH dG 15 000 sample 8 OH dG
24. http wako chem cOo jp SiyaKu jutaku index htm kDa 250 150 100 58 S0 37 5 We 20 15 10 kDa 2S0 1S0 100 7S 0 37 25 20 15 10 kDa 250 1S0 100 S0 37 25 20 1S 10 7 2 25 20 15 10 kDa 250 150 100 7S S0 37 25 Ee 20 200ug 200ug 200ug 100ul 100 100 5m 10m 100m 5m 5m 5m 20 FEB 2009 No 94 kDa 250 150 100 S0 25 20 15 kDa 1S0 100 75 50 37 25 20 15 30 000 30 000 30 000 30 000 33 000 33 000 33 000 15 000 19 000 27 000 120 000 29 500 25 000 25 000 3 000 HF Hako joWWindow 0HG 8 OH dG PHG 3 OHG 8 2 8 OH dG
25. 107g 10 Protein G slurry 207 SDS PAGE CE 100kDa Ago2 5X 106 1 1 000 014 22023 30 000 Anti Mouse Ago2 Monoclonal Antibody Clone No 2D4 018 22021 100 50 000 011 22033 50ul 30 000 Anti Human Ago2 Monoclonal Antibody Clone No 4G8 015 22031 100 50 000 292 67301 microRNA Isolation Kit Mouse Ago2 10 45 000 0 540 8605 1 2 06 6203 1788 8 103 0023 5 13 03 3270 8243 8 092 622 1005 082 285 6381 052772 0788 045 476 2061
26. KOR 2 leG FITC KOR HEK293T A GPR40 HEK293 F DOR 2 IgG FITC DOR HEK293T GPCR No 308 33601 KG116 Anti Mouse GPR40 Monoclonal Antibody Clone No G16 25g 60 000 306 35721 KG136 Anti Human TSHR thyroid stimulating hormone receptor Monoclonal Antibody Clone No 3A6 50 g 55 000 303 35731 KG137 Anti Human LGR4 leucine rich repeat containing G protein coupled receptor 4 Monoclonal Antibody Clone No 12H6 50ug 55 000 301 37591 KG138 Anti Human GPR120 Monoclonal Antibody Clone No 2B6 25u8 55 000 304 37601 KG139 Anti Human KOR Monoclonal Antibody Clone No 5E3 25u8g 55 000 J Re 508 38461 KG142 Anti Human DOR Monoclonal Antibody Clone No 1E7 25u8g 55 000 P
27. 3 5 6 3 3 57 129 3 26 28 2009 3 27 29 FEB 2009 No 94 e http Www siyaku com 7 2 NR Wako SW 1 CH 2 Na NH Cs C 0 3 CaH N O HCI 256 77 0 a 8 123 05001 ATP Wako STNP ATP a i N N 4 C HL saNHT P2X
28. CHSE 214 LNCaP C8B4 PC 12 3T3 Vero Nai HEK 293 0Oaco 2 BHK CHO Hep G2 PER C6 HTB 13 U 2 OS NSC 34 Calu 3 16HBE Transfected cell lines Saos 2 DLD1 MBDK Primary prostate Endothelial cells E4embryo Melanoma 2 4 A1 Preadiposites Cardiomyocytes A549 Primary rat liver HUVEC Primary mouse embryo fibroblast Hi 5 BTI Tn 5B1 4 HTC 116 HEK LNCaP 24 hr Initial Post Thaw LNCaP 7 Day Growth EC Ne ee 6 E a 3 A 1 GR GOBND CN 3 Treated Surface Treated Surface Tissue Culture Treated Corning CellBIND Surface LNGap 7 3 SE ep Gr a gt
29. New Product Guide 220 U S http wako chem co jp siyaku jutaku index htm FEB 2009 No 94 Hako joWWindow 4 MY G N FOS CE NC Et 2 96 D
30. gt PPP 0 0 TP DPR 66m Lysotracker Tr 35z 3706 ooo 2m AntiBleach eenssereesereeesertereesees teresa 1m 8 oo 8 07 ALZM008 http wako chem cOo jp SiyaKu jutaku index htm FEB 2009 No 94 Hako joWWindow CORNING CORNING CellBIND Surface 2 CellBIND Cell Line
31. No 3 642 07681 3289 CellBIND 25cm 200 54 200 649 07691 3290 CellBIND 75cm 100 43 700 641 10601 3073 CellBIND 100cm 60 52 200 642 07701 3291 CellBIND 150cm 50 48 300 649 07711 3292 CellBIND 175cm 50 51 150 646 07721 3293 CellBIND 225cm 25 26 400 648 08021 3298 CellBIND 175cm 50 48 850 645 08031 431328 CellBIND 175cm 84 96 600 644 09101 431346 CellBIND 235cm 42 47 460 647 09191 3068 CellBIND 10 50 37 500 643 09193 3067 CellBIND 20 100 69 300 645 10741 10010 CellBIND 1720cm 4 39 200 645 10981 10024 CellBIND 1720cm 24 223 2
32. A 10Lx5 107x5 107X5 2009 2 1 2009 3 31 3096 No RN 391 02021 17X20 7 000 Chu N6 Medium Salt Mixture RN 399 02027 107X5 12 500 eo 396 02037 107X5 12 500 8 750 Murashige and Skoog Plant Salt Mixture wa 392 00591 17X20 7 000 399 00621 Gamborg s B5 Medium Salt Mixture 17 X20 7 000 G K na 8
33. 029 858 2278 022 222 3072 011 Z71 0285 0720 052 099 0720 052 806 DM E mail biowin wako chem co jp URL http www wako chem co jp Wako Chemicals USA Inc http www wakousa com Head Office Richmond VA http www wako chemicals de Tel 49 2131 311 0 Tel 1 804 714 1920 Los Angeles Sales Office Tel 1 949 679 1700 Boston Sales Office Tel 1 617 354 6772 09221 2 o1DN e Wako Chemicals GmbH Neuss 4 dd FEB 2009 No 94
34. C3 clone 5F9 H00000718 MO1 E 3 g m FGF1 clone 2E12 E 34g m MARCKSL1 clone 8C8 H00065108 M02 3g m TP53 clone 2C11 HO00007157 M0O4 f 1 1 1 8 1 5 g m Abnova Antibody Innovation CDKN1B clone 4B4 E6 H00001027 MO1 a tn es Me we 5g m HMGB1 clone 1E6 E10 HO00003146 M0O1 E 3g m NME1 clone 1D7 H00004830 M02 E 3g m httD Www Siyaku com Mak jo Wdow 0 B2M
35. Immuno Skim 1 Reagent B enhancer milk A549 5g X1 10 g X2 SDS PAGE 39 TBS T EBB1 X500 HRP IgG X7000 6 9 294 68601 2 290 68603 mmuno enhancer 10 298 68604 40 K 0 http wako chem co jp siyaku jutaku index htm FEB 2009 No 94 Hako joWWindow a TAC Cry j1 ELISA Kit 2 Cry jl ELISA 0 156 10ng m 450nm
36. eee p 21 EAR USR 2 4 2 22SNI 2 0 D 21 2 ER ee p 22 LH p 22 3 p29 BioVision Glycogen Assay Kit ogoooooooooooooopoooooooooooo D 23 BioVision Choline Acetylcholine Quantification Kit p 24 Tocris p25 AxyGen Biosciences p 26 Cellufine Mini Column goocooooooopooooooopooooooooooo so p27 Osenses p 19 10LX5 ee Oe eT p 20 EE p 20 Tocris 3 Ne ceoR2Hoe GO D 25 Wako Hako joWWindow Anti Tag Mlonoclonal Antibody c Myc HA GST 6 x His GST 6 xHis c Myc HA
37. H00000567 M01 B2M monoclonal antibody M01 clone 3F9 2C2 47 000 BAG1 H00000573 M01 BAG1 monoclonal antibody M01 clone 4E2 47 000 H00000573 M02 BAG1 monoclonal antibody M02 clone 2D3 47 000 BMPRI1B H00000658 M01 BMPRIB monoclonal antibody M01 clone 2F3 47 000 H00000658 M02 BMPR1B monoclonal antibody M02 clone 3A8 47 000 H00000658 M07 BMPRIB monoclonal antibody M07 clone 2E2 47 000 C3 H00000718 M01 C3 monoclonal antibody M01 clone 5F9 47 000 CDKN1B H00001027 M01 CDKNIB monoclonal antibody M01 clone 4B4 E6 47 000 H00001027 M02 CDKN1B monoclonal antibody M02 clone 2F4 47 000 CLU H00001191 M01 CLU monoclonal antibody MO1 clone 1A11 47 000 H00001191 M02 CLU monoclonal antibody M02 clone 2F12 47 000 COX17 510 56841 H00010063 M01 COX17 monoclonal antibody M01 clone 4G2 47 000 H00010063 M03 COX17 monoclonal antibody M03 clone 1A9 47 000 DDX54 H00079039 M01 DDX54 monoclonal antibody MO1 clone 2H6 47 000 H00079039 M02 DDX54 monoclonal antibody M02 clone 2E4 47 000 H00079039 M03 DDX54 monoclonal antibody M03 clone 5B3 47 000 H00000355 M01 FAS monoclonal antibody M01 clone 5B4 47 000 FASLG HO00000356 MO1 FASLG monoclonal antibody MO01 clone 1E1 3C2 0 1mg 47 000
38. http wako chem co jp siyaku jutaku index htm FEB 2009 No 94 Hako joWWindow Boston Biochem BostonBiochem a 3 Atg12 Ate79 E1 Atg8 LC3 E1 E2
39. CellBIND 1700cm CellBINDY 850cm CellBINDY 1750cm hn TN 50 50 50 50 50 50 50 20 44 20 ll nak jo Wdow 24 200 26 400 32 200 30 500 25 850 87 750 104 800 48 000 91 520 85 500 641 07771 648 07781 645 07791 644 07803 648 07801 3330 3310 3311 3312 3320 CellBIND 1 CellBIND 2 CellBIND 5 CellBIND 10 CellBIND 10 8 5 2 2 6 69 600 60 000 48 000 78 400 220 800 Osenses Osenses 2007
40. 5 HT 10mg 41 700 a LY 310762 1D A DE a EE 0 4 MY Sold under license from Eli Lilly and company 00 50mg 179 000 517 64041 5 HT 10mg 47 000 IE LY 334370 hydrochl 8908098 2 ON OD ODO Sold under license from Eli Lilly and company 24998 50mg 212 000 516 64011 a a 10mg 33 000 toni 2 RD NG I 3017 1 Methylpsilocin 5 HT 1465 16 3 50mg 146 000 514 50761 2556 PNU109291 5 HTip 187665 60 7 10mg 54 200 517 50751 1985 PNU 142633 5 HTjp 187665 65 2 10mg 52 300 694 50 Re046700 5 HT 202466 68 0 Rg 99600 50mg 170 500 8 213 64001 2993 Rs 127445 hydrochloride 5 HT 199864 87 4 mg 25699 50mg 196 000 19 6 ssg sB399885 hydrochloride 5 HT 4029860 9 0000 50mg 212 000 511 64061 5 ht 10mg 48 900 B 1 5A 0 9 MA Sold with the permission of GlaxoSmithKline OD 50mg 220 000 3085 Ziprasidone hydrochloride 5 HT D 198982 67 9 ome 5 000 Tocris
41. Bax 1 g m A CO HRP GAR HRP 0 16g m B D Clean Blot IP Detection Reagent HRP 0 4g m Pierce ECL Chemiluminescent Substrate 32106 515 59951 21230 Clean BloLIP Detection Reagent HRP 100 2 5m 33 000 Clean Blot IP Detection Kit HRP 2 000cm 512 59961 21232 y Clean Blot IP Detection Reagent HRP 2 5m 1kit 60 500 y StartingBlock T20 TBS Blocking Buffer 17 ECL Detection Reagent1 125m ECL Detection Reagent2 125m 519 59971 21233 Clean Blot IP Detection Reagent AP 100 2 5m 33 000 576 31961 20421 Immobilized Protein A G Sm 65 800 518 59321 28376 BupH Tris Buffered Saline Packs 40 packs 40 PACKS 26 000 575 82771 39000 Lane Marker Reducing Sample Buffer 5X 5m 10 000 552 76521 37543 StartingBlock T20 TBS Blocking Buffer 17 30 000 550 71821 32106 ECL Western Blotting Substrate kit 24 500 U K CAAA Evrogen e BVPU BITI Gateway TagRFP Gateway TagRFP C N attL1
42. _ No 518 24031 GelRed Nucleic Acid Gel Stain 10 000Xin DMSO 0 5m 29 500 519 20301 41003 GelRed Nucleic Acid Gel Stain 10 000 Xin Water 0 5m 31 000 551 93331 41004 GelGreen Nucleic Acid Gel Stain 10 000 Xin DMSO 0 5m 25 100 517 53333 41005 GelGreen Nucleic Acid Gel Stain 10 000 Xin Water 0 5m 28 000 U TN FEB 2009 No 94 http www siyaku com 727oZ 27477 8 8 9 MP Biomedicals
43. No 291 50502 DNA DNA DNA Extractor WBKit DNAExtractor TIS Kit P 8 OHdG Check No 307 07921 8 OHdG 296 67701 DNA ExuactoWTISKt 30000 a ELISA 8 OHdG Mt ELISA 2 8 OHdG Check 8 OHdG Check 8 OHdG Check 0 5 200ng m 8 OHdG Check 0 125 10ng m 307 07921 8 OHdG Check 96 80 000 301 06101 8 OHdG Check 96 80 000 aa gt 8 OHdG DNA _ 8 OHdG Assay Preparation Reagent Set _ 8 OHdG 8 2 a DNA DNA 50 0 G T
44. attL2 TurboGFP Gateway Technology Gateway destination vector NR MCS PUEeri TagR FP AS pucaori TagRFP AS Gateway Gateway TagR FP AS C TagRFP AS N entry clone MCS RoE 4kb _ Stop NB 7 attL2 C 4 4 kb attL2 NN Kan Meo Kan Nen K 1 F P 0 TagRFP AS C entry clone Gateway TagRFP AS N SY clone FP148 Gay TagRFP AS C entry FP149 Gateway TagRFP AS N entry clone I PP521 Gateway TurboGFP C entry clone 84 000 554 78281 FP522 Gateway TurboGFP N entry clone 84 000 Evrogen http www evrogen com Gateway destination vector URL http tools invitrogen com content sfs manuals gatewayman pdf Evrogen Notice
45. 100 30 GelRed GelGreen a 66 ya EtBr CCR title22 WD 50m 2 DD 90 EN EPA SW 846 GelRed GelGreen GelRed EPA SW 846 Dn pH EPA 9040C 4 0 5 3 ASTM D 93 gt 1502F 65 6C 2l509 6560 19 CCR Biotium EPA ASTM 200 300 4nQ 50n 500 700 Biotium nm URL http w
46. TIE1 H00007075 M01 TIE1 monoclonal antibody M01 clone 3F4 47 000 TP53 HO00007157 M01 TP53 monoclonal antibody M01 clone 2C3 47 000 HO00007157 M04 TP53 monoclonal antibody M04 clone 2C11 47 000 http www abnova com FEB 2009 No 94 e http www siyaku com 727oZ 27477 RI Proteome Profiler 15 ME 2 A1 A2 Control 15 2 A7 A8 Control D3 D4 Otx2 Bl B2 Oct 3 4 D5 D6 TP63 TP73L ee B3 B4 nanog D7 D8 Goosecoid GSC B5 B6 SOX2 E1 E2
47. FEB 2009 No 94 http www siyaku com 7 2 jP NFzB Bax A549 4g 4C A G 20421 Binding Wash Buffer 28376 3 Binding WashBuffer A G 100 2 2 500 Xg 1 Binding Wash buffer 500u7 3 Bax NFxB A G 5XLane Marker Reducing Sample Buffer 150 39000 5 HBPES Precise Protein Gels 25244 PVDF 88518 StartingBlock T20 Blocking Buffer 37543 NFxB 1g m
48. Atg12 Ats12 Atg7 B1 Atg10 B2 Atg5 Atg12 Atg5 Atg16 Atg8 LC3 Atg8 LC3 C Ats4 Atg7 E1 Atg12 Atg3 E2 PE Atg4 Atg8 LC3 PE Reagents Boston Biochem Atg Apg UL 410 His Apg8p1 GABARAP human recombinant C0 500ug 30 000 UL 420 His Apg8p2 GATE 16 human recombinant Co 500g 30 000 UL 430 His Apg8p3 MAP LC3a human recombinant E Co 500 g 30 000 Derivatives a UL 412 Biotin Hisg Apg8p1 GABARAP human recombinant Co 50g 35 000 UL 414 Fluorescein His Apg8p1 GABARAP human recombinant Coli 50 g 35 000 UL 416 Rhodamine Red His Apg8p1 GABARAP human recombinant E Co7 5
49. B2M clone 3F9 2C2 H00000567 MO1 E 3g m COX17 clone 4G2 No 510 56841 E 5g m HSD17B1 clone 2E5 H00003292 M03 3g m PGR clone 3E11 HO00005241 MO01 i 5 E 3g m FEB 2009 No 94 BAG1 clone 2D3 H00000573 M02 34g m DDX54 clone 2H6 H00079039 MO1 34g m KRT18 clone 2F8 H00003875 M01 3g m TP53 clone 2C3 3g m
50. P2X L P2X P2X ATP NN ON ATP 11 23mg m 0o o o HO P O P O P O oN O O O COX 1 Wako 3TFAP M 5 2 4 COX NH COX 1 COX 2 NSAIDs COX COX 2 HN NN COX 1 COX 1 O TFAP COX 1
51. CONTENTS a Anti Tag Monoclonal Antibody c Myc HA GST 6XHis p 2 OHG 8 OH dG p 4 DNA SC D 5 ELISA 8 OHdG tt p 5 Anti Mouse Ago2 Monoclonal AntiDodWV t D 28 Boston Biochem bi ee Immuno enhancer D TAC Cry jl ELISA Kit ee p 8 TACS DerfIL ELISA Kit ee p 8 GPCH CCICEO CGC 000 p 9 Abnova ee p 10 R amp D Proteome Profiler D 13 Pierce Clean Blot IP Detection Reagent HRP AP p 14 Evrogen Gateway TagRFP p 15 Biotium GelRed amp GelGreen 0 p 16 MP Biomedicals p 17 FEB 2009 CellBIND Surface ee p 18 Chu N6 Basal Salt Mixture m D 20 4 ee p 21 TNP ATP
52. 20 000 sample DNA 8 OH dG 40 000 sample FEB 2009 No 94 e http www siyaku com 7 2 8 OHdG DNA Wako cg DNA DNA TIS DNA DNA DNA 8 OHdG 8 2 8 OHdG Check 8 OHdG DNA DNA DNA 8 OHdG DNA Extractor TIS Kit DNA Extractor oo WB Kit
53. 620nm 2 40 Well 8 3 5 CV 596 4 4 CV 5 HBL C 1 Cry jl1 Cry j a TAC Derfll ELISA Kit Der fI ELISA 0 78 50ng m 450nm 620nm 2 20 Well
54. 0 54g m HA 5ng Anti GST Monoclonal Antibody Peroxidase Conjugated No 011 21891 1 1 000 GST 100z7 10 10u7 Anti c Myc Monoclonal Antibody Peroxidase Conjugated No 014 21901 1 1 000 c Myc 10ng Anti HA Monoclonal Antibody Peroxidase Conjugated No 011 21911 1 500 HA 10ng Anti GST Monoclonal Antibody 013 21851 010 21861 Anti 6x His Monoclonal Antibody 017 21871 Anti c Myc Monoclonal Antibody 014 21881 Anti HA Monoclonal Antibody 011 21891 Anti GST Monoclonal Antibody Peroxidase Conjugated 014 21901 Anti c Myc Monoclonal Antibody Peroxidase Conjugated 011 21911 Anti HA Monoclonal Antibody Peroxidase Conjugated ml Metal Chelate Agarose Set Ni Co Cu Zn RI 298 67401 Ni Co Cu Zn 1m 1 145 07981 141 07983 Ni Agarose 149 07984 031 19781 Co Agarose 038 19791 Cu Agarose 263 01871 Zn Agarose 294 02241 WakoPURE Spin Empty Column
55. 1 H00000356 M02 FASLG monoclonal antibody M02 clone 2G9 G8 47 000 H00000356 M03 FASLG monoclonal antibody M03 clone 3F3 47 000 FGF1 H00002246 M01 FGF1 monoclonal antibody MO1 clone 3F5 47 000 HO00002246 MO02 FGF1 monoclonal antibody M02 clone 2E12 0 1mg 47 000 H00002246 M03 FGF1 monoclonal antibody M03 clone 1F9 47 000 GAPDF 47 000 47 000 HMGB1 47 000 512 51281 H00003146 M02 47 000 HSD17B1 H00003292 M03 HSD17B1 monoclonal antibody M03 clone 2E5 47 000 ID2 HO00003398 MO1 ID2 monoclonal antibody MO01 clone 3C3 0 1mg 47 000 H00003398 M04 ID2 monoclonal antibody M04 clone 2C11 47 000 IL2RA H00003559 M04 IL2RA monoclonal antibody M04 clone 1D6 47 000 http wako chem co jp siyaku jutaku index htm FEB 2009 No 94 Hako joWWindow ra IL6R No H00003570 M01 IL6R monoclonal antibody MO01 clone 2G6 0 1mg 47 000 H00003570 M02 IL6R monoclonal antibody M02 clone 1E11 47 000 IL6ST H00003572 M02 IL6ST monoclonal antibody M02 clone 4A4 47 000 2 H00003572 M05 IL6ST monoclonal antibody M05 clone 2A4 47 000 ITGA6 H00003655 M01 ITGA6 monoclonal antibody M01 clone 4C1 47 000 K
56. 2 IP 150 100 Ago2 100kDas mn a a 25 1 1 P388D1 SNS Protein G slurry 20g SDS PAGE 100kDa Ago2 5 107 1 1 000 1 200 1 1 000 1 100 1 500 5 10ug IP Mouse Hamster Rat Human P388D1 NIH 3T3 CHO SCC 131 NCI H460 x x microRNA O x a NIH 3T3 NT NT x NT Non Tested 2 deck2020 Ago2 SDS 0 kDa 1 2 3 1 2 3 Lane1 NIH 3T3 TP Lane 2 SCC 131 IPF Lane 3 CHO IP 150 a As02 100kDa 100 75 50 37 2 NIH 3T3 SCC 131 CHO
57. Snail B7 B8 E Cadhern B3 E4 VEGE R2 KDR FIlk 4 1 OO C3 C4 GATA 4 E7 E8 Control C5 C6 HNF 3 FoxA2 Fl F2 Control C7 C8 PDX 1 IPF1 gt Human Pluripotent Stem Cel ATray eee 8 Human Pluripotent Stem Cell 1 Array Buffer ii Array Detection Antibody Cocktail Array Buffer 2 Concentrate BX eeeeeeeeeeeeeeeeeeeeeeeeeeeeeee eee Streptavidin HRP Ne CE CEE Array Buffer ii 8 Well Rectangular Multi dish ene Lysis Buffer 16 ht gt Transparency Overlay Template RMR SRR UR 7G DOMINIO Wash Buffer Concentrate 25 Xe 2 ee LZ A 1 NN ee Pixel Density ET a or Pixel Densily Pixel Density Pixel Density 3 tn 1 Oct 3 OX17 ES E ES BG01V
58. throughput Hanging Drop 100lids 81 300 U K FEB 2009 No 94 e http www siyaku com 7 27477 Cellufine Mini Column 1m7 5m Cellufine 2004 M UNI TT _aullnlls gt 1m 5 ye y
59. to Purchaser Evrogen Fluorescent Protein Products the Products are intended for research use only The Products are covered by U S Pat 7 417 131 and other Evrogen Patents and or Patent applications pending By use of these Products you accept the terms and conditions of the applicable Limited Use Label License http Www evrogen com products Evrogen FP license shtml U MX http wako chem co jp siyaku jutaku index htm FEB 2009 No 94 HF Hako joWWindow A pa GelRed amp GelGreen Biotium lnwing Frnducls for Science 2 EtBr 1
60. 0 g 35 000 UL 422 Biotin His Apg8p2 GATE16 human recombinant E Coli 50ug 35 000 UL 424 Fluorescein His Apg8p2 GATE16 human recombinant Co 50ug 35 000 UL 426 Rhodamine Red Hisg Apg8p2 GATE16 human recombinant E Co 50 g 35 000 UL 432 Biotin His Apg8p3 MAP LC3a human recombinant E Coli 50ug 35 000 UL 434 Fluorescein His Apg8p3 MAP LC38 human recombinant Coli 50 g 35 000 UL 436 Rhodamine Red His Apg8p3 MAP LC3a human recombinant Co 7 50 g 35 000 Activating Enzymes E1 FEB 2009 No 94 e http www siyaku com 7 2 Conjugating Enzymes E2 2 2 too HI il EM Affinity Matrices UL 415 Apg8p1 GABARAP Agarose human recombinant E Coli 0 5m 30 000 UL 425 Apg8p2 GATE16 Agarose human recombinant RR Col 0 5m 30 000 UL 435 Apg8p3 MAP LC3a Agarose human recombinant Co 0 5m 30 000 U T _
61. 00 No 649 08551 3294 CellBIND 35mm 210 13 860 646 08561 3295 CellBIND 60mm 126 10 080 643 08571 3296 CellBIND 100mm 40 6 720 FEB 2009 No 94 e http www siyaku com No 644 08501 641 08511 648 08521 645 10361 647 07751 644 07761 642 08041 3335 3336 3337 3338 3300 3340 3683 No 641 08011 646 08583 649 09531 No 431134 431344 431371 CellBIND 6 CellBIND 12 CellBIND 24 CellBIND 48 CellBIND 96 CellBIND 96 CellBIND 384
62. IT H00003815 M01 KIT monoclonal antibody M01 clone 6G12 47 000 KRT18 H00003875 M01 KRTI8 monoclonal antibody MO1 clone 2F8 47 000 H00003875 M03 KRT18 monoclonal antibody M03 clone 1G8 47 000 2 NAP2K7 H00005609 M01 MAP2K7 monoclonal antibody MO1 clone 4E8 47 000 MARCKSL1 H00065108 M02 MARCKSL1 monoclonal antibody M02 clone 8C8 47 000 H00065108 M03 MARCKSL1 monoclonal antibody M03 clone 2H5 47 000 NFYB H00004801 M01 NFYB monoclonal antibody M01 clone 6H6 47 000 NGFB H00004803 M01 NGFB monoclonal antibody M01 clone 2H4 47 000 NME1 H00004830 M01 NME1 monoclonal antibody M01 clone 2H1 47 000 H00004830 M02 NME1 monoclonal antibody M02 clone 1D7 47 000 PGR H00005241 M01 PGR monoclonal antibody M01 clone 3E11 47 000 PTEN H00005728 M01 PTEN monoclonal antibody M01 clone 2G9 47 000 H00005728 M02 PTEN monoclonal antibody M02 clone 3E7 47 000 RPS27A H00006233 M01 RPS27A monoclonal antibody MO1 clone 3E2 E6 47 000 H00006233 M02 RPS27A monoclonal antibody M02 clone 2G10 47 000 S100A2 HO00006273 M01 S100A2 monoclonal antibody M01 clone 2D10 A3 47 000 H00006273 M03 S100A2 monoclonal antibody M03 clone 3H8 47 000 H00006273 M06 S100A2 monoclonal antibody M06 clone M2 47 000
63. ase Conjugated YPYDVPDYA SN Anti 6XHis Monoclonal Antibody No 010 21861 C 6XHis ER 293T ProteinA G SDS PAGE Anti GST Monoclonal Antibody No 013 21851 Anti c Myc Monoclonal Antibody No 017 21871 IP IP kDa 1 2 3 4 5 kDa 1 2 3 4 5 34 Gh 50 1 293T EE 1 293T 43 2 lug m7 26 s 2 lug m7 3 2ug m7 3 2g m 4 S 2g m 4 S 2g m 34 5 lgG 5 lgG Anti 6XHis Monoclonal Antibody No 010 21861 Anti HA Monoclonal Antibody No 014 21881 JP JP kDa 1 2 3 4 5 6 7 kDa 1 2 3 4 5 N 2 5g m 1 6XHis R 1 293T
64. roteintech GPCR 3 No 65008 1 lg Anti Human CXCR3B Monoclonal Antibody 150z 68 000 60042 1 lg Anti Human CXCR4 Monoclonal Antibody 1507 68 000 60046 1 lg Anti Human CCBP2 Monoclonal Antibody 150 68 000 50650 1 AP Anti Mus musculus Ccr5 Polyclonal Antibody 150ul 68 000 51024 1 AP Anti human CXCR7 Polyclonal Antibody 150z 68 000 11073 2 AP Anti human CXCR4 Polyclonal Antibody 1507 68 000 11656 2 AP Anti human MCHR1 Polyclonal Antibody 150z 68 000 10163 1 AP Anti Human GPR24 Polyclonal Antibody 150ul 68 000 51029 2 AP Anti Human SSTR5 Polyclonal Antibody 150z 68 000 11300 1 AP Anti human P2RY6 Polyclonal Antibody 150ul 68 000 G K http wako chem co jp siyaku jutaku index htm FEB 2009 No 94 ee Mako BjoWindow Abnova Abnova 1IHC ELISA
65. uffer Hydrolysis Enzyme Mi vial 30 gt Development Enzyme Mi vial Glycogen Standard 2 0mg m BORN 100 Development Buffer Development Enzyme Mix Develonment a Buffer OxiRed Probe DMSO 10 ia 570mm ExyEm 535nm 587nm http wako chem co jp Ssiyaku jutaku index htm FEB 2009 No 94 Hako joWWindow 1 NR a BioVision A 4 570 nm 7 CN _ Bx Em 535 590 nm
66. ww biotium com EtBr DNA 200zg 100zg 50ug 25ug GelRed GelGreen HeLa EtBr GelRed EtBr GelRed 0 5 ln a gt GelRed GelGreen 2 0 GelRed EtBr EtBr 2 312nm 300nm GelRed GelGreen EtBr dsDNA ssDNA RNA EtBr GelRed GelGreen GelRed V GelGreen PCR
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