Hybridization Solution
Contents
1. Non RI Alu DNA 100 9 ml2. Non RI Label Probe DNA 1 2x10 cpm ml or 1 10ng ml Non RI DNA 0 2 1ng ml RNA Southem Southen Norhem 1 2 4kb Non RI 4kb S N
3. Non RI RI 2 5 5pmoles ml 0 5 lt 1pmoles ml 1 10cm x 10cm 100cm9 5ml PerfectHyb 20 2 PerfectHyb 10cm x 10cm 100cm9 5ml 3 2 4 1 lt 2
4. 100ml 55ml 20 x SSPE 10ml 109 SDS 5ml 30ml B 0 1 x SSC pH7 0 0 1 SDS 1 2 10cm x 10cm 100cm 10ml 3 68 1 lt 2 4 B 68 10 5 X 6
5. SSC MagExtractor PCR amp Gel Clean up 200 NPK 601 25 000 Magical Trapper MGS 101 38 000 RI 50 gu 400 13 530 8230 2 8 TEL 06 6348 3786 FAX 06 6348 3833 103 8530 17 9 TEL 03 3660 4819 FAX 03 3660 4951 TEL 06 6348 3888 FAX 06 6348 3833 9 00 12 00 13 00 17 00 e mail techosk bio toyobo co jp URL http www toyobo co jp seihin xr
6. ii 1 tete 3 DNA RNA reer 4 eter 7 Appendix PU SSSEEEEEEECEDEDLPLPHPEPHPHPPPPPHPPPHPTPPPPPPPPPPPPPPPPPPPPPCTPPPPPPPPPPPPPPPPPPHTE 9 te 10 RI DNA EC CCP 11 CO 1 2 PerfectHyb Hybridization Solution Northern Southern blot RI Non RI Salmon sperm DNA Northern blo
7. Northern blot MM 80 Northern Southern 60 40 20 0 0 2 4 6 8 10 12 14 16 18 hr 1 Non RI DNA Northern blot actin cDNA Probe Southern blot VNTR Probe Total RNA 5g Genomic DNA 1 g 120 100 0 PerfectHyb 60 Conventional 40 0 2 4 6 8 10 12 14 16 18 hr 2 RI Transferrin receptor mRNA Total RNA 5 gg Molecular cloning 7 52 50
8. Non RI RI DNA RNA Northern blot
9. 6 68 5 2 7 68 C 15 2 8 RI X Non RI Non RI RNA Tm RI
10. SSC 0 1 x 0 2 x Non RI PerfectHyb Non RI 12 4kb
11. 10 _RI DNA _ RI DNA MagExtractor PCR amp Gel clean up Code No NPK 601 25 000 400 MagExtractor PCR amp Gel clean up Code No NPK 601 75 Magical Trapper Code No MGS 101 1 DNA 50 200 2 15 Il 2 3 4 300 10 5 6 759
12. Tm 18b Tm A T x 2 C G C x 4 18b Tm 81 5 16 6 log10 NaT 0 41 9 G C 600 N A A T G C G C G C N Na 0 75M PE Tm 10 C A Tm 10 C A Non RI A A Tm 70 55 60 RI
13. RI RI 1 10cm x 10cm 100cm 5ml PerfectHyb 68 C 20 2 5 3 68 C PerfectHyb 10cm x 10cm 100cm2 5ml 4 3 5 68 1
14. RI 4kb Probel Label Hyb 1 h 5 15 We cam xan Non RI RNA i 68 C B Southern a 68C 68 C A 68 C B Non RI A A B B RI
15. 5 5 2 6 10 2 7 RI X Non RI Non RI APPENDIX 20 xSSC 3 1 0 SDS A B 20 x SSPE 3M NaCI 0 3M lt 1L gt 1 175g NaCl 88g 900ml 2 1N HCI pH7 0 1L 3 lt 500ml gt 1 50g SDS 500ml 2 2x SSC 0 19 6SDS lt 500ml gt 1 50ml 20 x SSC 5ml 1092SDS 445ml 2 0 1 x SSC 0 1962SDS lt 500m
16. 1ml 10 E 7 8 lt 9 25 50 2 10 11 9 10 1 5ml 11 11 RI
17. A 2 x SSC pH 7 0 0 1 SDS B 0 1 x SSC pH 7 0 0 1 SDS Southern blot RNA Northern blot P9 X DNA RNA DNA RNA p7 RI
18. 02 03 PerfectHyb Hybridization Solution Code No HYB 101 TOYOBO CO LTD Biochemical Operations Department OSAKA JAPAN 37 RI RI RI
19. l gt 1 2 5ml 20 x SSC 5ml 10 SDS 492 5ml 2 3M NaCl 173mM 25mM EDTA lt 1L gt 1 175g NaCl 27g 7 4g EDTA 2Na 800ml 2 5N NaOH pH7 4 1L 4 w UV RI DNA
20. t DNA RNA RI cDNA Non RI Southern blot DNA Non RI
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