1、 システムの起ち上げ（電源の入れ方）
Contents
1. Acquisition Mode AM Average Fig18 Line 4 Mode Line Frame Method Mean Sum Number Page 7 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 2 Channels Mode Acquisition Channels w Channels Channel Ch2 Pinhole 1 00 Airy Unis 11 6 pm section Ga n Amp Offset Track Tracki wv 458 477 4865 514 561 633 800 Status Transmission 488 nm 633 nm Fig19 Channels2. Light Path ZEN Page 8 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 Workspace Configuration 2
3. Page 27 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 fig 30 OD fig30 LSMD 2 I lism xWindows Zeiss Image Browser http www zeiss de ImageBrowser I E
4. Single plane 1 BURE Series Movie 1 Compress Page 30 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 I I File Browser ZEN File Browser Ik ZEN Ctrl F File Menu File Browser Explorer x Image Database IE ZEN x File Browser File
5. on off Page 9 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 4 Single Track Simultaneous CHH 138 LSM5EXCITER I l IT l IT RRI ORARIA Lt LF RNR O l IV Acquisition Fig8 Acquisition m Angustin Paat Find Connie Setup Manager 0 Laser DE Imaging Setup 0D p Ugh Path Online Acquisition D Acoulsition Mode 0 Channels Focus u A298 i Regions Mulidmensional Ae CL hc D Z5 Bleaching D o Time Sakes Or The San 0 i Information On Experiment Page 10 Carl Zeiss Co Ltd LSM35EXCITER ZEN 2007 4
6. Detector Gain amp Ampl Offset 4 W 2 Detector Gain Amp Offset Find Saturation 100 Detector Gain Ampl offset ZEV Continuous Acquisition Acquisition Channels E stol Channels Detector Gain Ample Offset Channel Ch2 Pinhole Gain Amp Offset Intensity0 Track Tracki 458 477 633 800 Status DE Transmission
7. Ocular Setup manager Light path Light Path E Light Paih Congo m re o 00 1 reny ee Focus u Aperture 0 3911 1 BF US on off mx Page 12
8. Section Single Page 26 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 6 HHEBRORT HBORFERAUL a Single om lt Start 2D Time New
9. Multi Channel Acguisiton Oz Z Stack Mode Mode First Last Center Mark First Last Z VIDdEHEARNHAHK TT EE DL Seas 2 Interval 1 044 z Keep interval Slice Optimal Interva 3 08 ym Continucus oL Set Last 20 241 Set First 25 695 Range 45 94 um Focus Position 2 73 um een Set First Z Set Last Z B Stop Fig39 Z stack Z stack Navigation A bi Page 33 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 Sectioning Scan
10. DIE DIC 1 2 0 PC ALEF 2 UIC DIC DIC HI 40 DICH 40 1 DIC DIC DIC
11. Rhodamine FITC 14 i y t Fitc Ex gt Fitc Em HE 1 1 s J ti Multi Wrack AE Multi Track 1 Line p Frame Page 23 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 Single Track Configuration Find amp Multi Track Acquisition Imaging Setup
12. 1 Airy Fig19 Page 18 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 4 IV 1 Find Image acduisition Find Fast Continuous Single Stop
13. Multi Track GECTERSNNEGEN Mode Channel Mode Switch track every Frame re Tracki Ch GF Imaging Setup S p Configuration NG Single Track Alexa 488 Cy3 Such rackevery RRS Multi Track Multi NLO i Fi Tada NLO 2 Load Mi test meta fig25 Mode Page 24 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 Store Delete Find Multi Track Find
14. Intensity Acquisition Mode w Acquisition Mode Objective Plan Apochromat 63x7 1 4 Oil DIC Scan Mode Frame T ii Frame Size k 512 Pixel Dwell 1 60 psec Scan Time 3 93 sec u Averaging Number O San Area zia 4 Aquisition Mode Single 4 2 Page 22 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 5 Multi Track Sequential CHE 478 LSM Single Track Multi Track 2 gi Aa Rhod 5 N a Be es An 1 Single Track
15. 30 21 2 Pro Mode Basic Mode 5 Time Series Cydes Interwal Fig6 Page Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 ZEN Fig6 E Light Path Acquistion Fig7 ZEN
16. Warge fia26 2D Marge Dimension Overlay on off Page 25 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 fig27 Split Detector Gain Amp Offset Multi Track Detector Gain amp Ample Offset ww Channels Chamirk1 Ch Pinhole i 1 00 Airy mC pemean D TAU Gain Amp Offset Track Tracki ww W 458 AT 48 514 561 633 8p Status Transmission S 488 nm 633 nm fig28 Channel Gain amp Amp Offset
17. Page 39 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 LSM Page 40 ZEISS Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 1 Ph 1 3 Ph Ph 1 2 Page 41 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 8 IV ON 1 2 3
18. 512x512 0ptimal N A Acquisition Mode Speed 1 10 8 9 7 Acquisiton Mode AA Bit Depth 8bit 256 12bit 4096 2
19. U fig30 2 a fig30 3 Page 28 Carl Zeiss Co Ltd LSMS5EXCITER ZEN 2007 Save Save peichern unter ar Speichern E Desktop f Eigene Dateien demo data W Arbeitsplatz G JenaKulur Netzwerkumgebung ee Zeiss EC CiscoSecure Authentication Agent CL 4 2 Icons ee a Bee LSM lsm la Dateiname image 2 Dacbp SHE Abeechen Save IL 1 Export TIFF JPEG etc Tagged Image FI File Menu Wb Export
20. A C D E 3 F Fig4 ZEN 6 7 8 9 5 10 Fig5 ZEN Page 6 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 3 VIKRDLIFOBAHRT ZEN ZEN LSM5 ZEN
21. 3 Fig4 D Fig5 1 Fig5 1 overlay 3 ZEN
22. Power Enable OFF 2 OFF 3 LSM File Exit Start Exit 4 Windows Start gt Shut Down PC OFF 5 OFF 6596 309 5 15 Page 43 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 Page 44
23. Fig2 ZEN Start System Login ZEN 300 xX Laser Scanning Microscope LSM 510 0 Boot Start System Image Processing Login ZEN 2007 Laser Scanning Microscope LsM 510 Boot Status CP DIOs CP Video Swilches CP Cameras ICP HRZ a CP Focus Tg Start System Fig3 C Start System Image Processing Page 4 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 Boot status c Page 5 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 ZEN Fig4 5 gt ZEN
24. ra _ n Switch track every Frame rn Track1 Ch Fig15 Imaging Setup w Imaging Setup Configuration Ill Single Track Alexa 488 Cy3 seq 1 Z Ai Alexa 488 Cy3 sim Multi Track Multi Switch track every duo seg NLO 1 le Tada NLO 2 ose 1 ose P Jasers on Mode ch2 test meta z stack config Fig16 Page 14 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 Load E Store Delete ka UV 405nm DA B FITC Cy2 G Rhodanin Cy3 R Cy5 Setup Manager
25. AOTF Excitation Page 20 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 Fig21 Channel Gain Ample Offset RHR NTRERDET Ample Offset Transmission Excitation AOTF 488nm 5 gt 10 12 m EB 4 2 _T_ O Mr PM _ u _ A f hr IX VI RRE IL maye IIIUI dO TH KI DELELLUT Bd AIIID VISEL TITE gJ View Dimension
26. 4 HBO Field Stop Aperture Stop FL P amp C X N A a A oQ N e Page 42 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 9 ER TADKT EIROOFF 1 OFF HeNe543 HeNe633 90 Ar Standby OFF OFF 5 D 5
27. 0N Axio Observer 1 6 6 TFT Page 35 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007
28. Power Enable ON 90 Standby F on Power 9 10 LED LED 2 1 PC 2 3 77 97 MIF POEM OiR Page 2 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 2 10 Page 3 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 2 INRL TIE2TIYTIKDESLIf LE Ben Fig1 ZEN 2007
29. g Wew Dimensions Ch rn fig22 Range Indicator I fig22 View Dimension 1 Range Indicator 0 Detector Gain Amp Offset fia23 Ranae Indicator Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 1Airy fig21 Detector Gain 1000 1100 fig 21 Channel ig21
30. off Page 11 Carl Zeiss Co Ltd LSM35EXCITER ZEN 2007 4 I Microscope Setup Fo 0cular Camera LSM Ocular TF Camera CCD LSM Fig10
31. 26 Page 37 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 8 1 1 0cular 2 TFT HAL on off ML Si ON 3 H 3 LD a Aa Page 38 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 53 8 IT 200um
32. Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 Fig11 AxiolmagerZ1 w E Lighi Paih m CHEREBOEYFL Y Bo X cite 120 Conkguralion 6 or HBO100 er I M ER B Clgsad 100 38 A go pan on 10 0 MAT es 0 3911 1 ML Fig12 Reflector Turret H RBREDKFEIFUHL figl3 U Load Save LIJ SE Light Path Ocular Configuration Page 13 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 Fig13 4 I Conf
33. Contents of image window single pare Raw data series Contents of image window single plane Contents of image window series Full resolution image window angle plane Full resohution image window series Select file name and save Selat Ale name and save Fig33 Export Format Data Raw data Contents of image window Ortho Page 29 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 Full resolution image window image window 1024x1024
34. Fast Continuous Detector Gain Amp Offset var io Fig20 Image Display Detector Gain Z Continuous Detector Gain Page 19 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 a 1 Average Fast Continuous a Find
35. New File Browser wN ZEN 2007 Light Edition File Edit Window Help Fig34 File Menu File Browser Op en m HDD CFP DAPI Alexa546 YFP CFP DAPI Page 31 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 Fig35 File Browser I 1 File W Open N ZEN 2007 Light Edition File Edit Window Help N ZEN 2007 Light Edition Fle Edit Window Help w B Fig36 File Menu Fig87 File Open l
36. Page 36 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 1 2 3 L 4 5 6 7 8 01 2 9 10 25mm 11 2 13 LM set 14 X Y 15 16 L 17 18 BER OnOff 2 3200 19 On Off 20 TFT 21 22 MER 23 24 25
37. 1 Laser Control Exciter TWLFDHTOL F ERISEUEF Setup Manager Laser LSM510 510META DPSS 501 10 561 Chameleon 720 930 HeNe633 633 le Laser Informat on Max mum Power 30 0 mW Wavelength 458 ATT 488 514 nm Status Warming up Tube Current 60A Output Fig9 LSM510 510META Blue Diode 405 405nm standby 5 on Argon 2 458 477 488 514nm standby 5 on Output 509 6 HeNe 543nm 594nm 633nm on 10 off
38. Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 1 ERTLADESLIT EROIAT 1 2 On 3 PC 4 Ar HeNe 1 A 3 PC Windows Log in 4 HeNe 543nm G HeNe 633nm R ON Page 7 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 405nm V 90 Ar 458 488 514nm B Ar
39. Light Path Light Path fig17 gr C Light Path BP 500 550 IR Marar EP NFT 565 BP 650 710 IR F Le HFT 4585616 o 33 Laser gp Nong Fig17 Single Track Light path LSM HFT NFT Page 15 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 LP BP 4 IV Scan Control
40. Optimal Interval Z ERNEST TE ks Optical Slice Optimal Interval 3 27 MM Z D un Start Z Average x Z ZEN LE Page 34 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 8 KFRAMEI LS SHE ALA Axio Observer ZI LSM
41. 21x E Biomed lt e eE Biafilm lsm CRmouseFITGTRITG sm 21 BioMed mdb Maus DIC Haarzelle m CytoskeletonDNalsm MDCKcells lsm DaPleCFPAlexa546 lsm DaPleGFPAlexa546 lsm drosophilaprojektion Ism ICytoske letonDNA lsm 0 p Fig38 File Page 32 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 7 DIBEOFRHKIZILT Z 1 2 Sectioning Scan 3
42. 1 Acquisition Mode 2 Channels amp AOTF 1 2 1 Acquisition Mode Acquisition Acquisiton Mode Acquisition Mode Objective Plan Apochromat 63x 1 4 Oil DIC Scan Mode Frame Frame Size Y52 Optimal Speed Pixel Dwell 1 60 psec Scan Time 3 93 sec Averaging Number Bit Depth 86Bit Scan Area gt Fig18 Acquisition Mode AxioSkop2FS Optimal TOE Page 16 ZEISS Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 Frame Size
43. iguration LM 2 Single gc Multi gc 5 MultiTrack DZETEROKENTEH LET M Workspace Zoom Ocular Camera Fig14 LSM Single Track Acquisition Imaging Setup Single Track Imaging Setup en all
44. xport 2 lsm C D LSM5 D I lsm File Menu Save Save as ZEN
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