“WAKO BIO WINDOW” No.72(2005.12)
Contents
1. 1 30 DNA 2m7 50 g DNA A260 280 1 97 A260 230 2 04 PCR Na gp 531 N 3 Ne_ 9 633 07311 QuickGene 610L 1480 000 630 07321 QuickGene DNA L 48 44 800 G K vbo Cybox Co Ltd2. 80 wm 8 QuickGene 800 UM re A 1 1 gt 1 1 4 w yn Fr 4 DNA WHOLE BLOOD DNA RNA RNA RNA PLUS RNA DNase
3. Cell Line Nucleofector C Kit CCRF CEM NSO cells um Cell Line Nucleofector L Kit A 10 EL4 OMDCKcells BHK 21 FDC P1 WEHI 231 cells DU 145 25 p Cell Line Nucleofector Solution 2 25m 3 Supplement RR 8 i Ss 0 5m pmaxGFP Et EE 88 30 lu 9g 2 Certified cuvettes NE 25 CCRF CEM NSO cells A 10 BHK 21 DU 145 EL4 FDC P1 MDCK cells WEHI 231 cells y Plastic pipettes ee 25 pmaxGFP 24 No 553 81201 VCA 1004 Cell Line Nucleofector9C Kit 25 NEW 550 81211 VCA 1005 Cell Line Nucleofector L Kit 25 ROCK Wako Y 27632 Y 27632 RA trans N 4 pyridyl 4 1 aminoethyl cyclohexanecarboxamide 2HCI HzO ROCK Rho associated coiled coil forming kinase Rho ROCK p160ROCK Ki 140nmo1l 7 1
4. ke 96 X1 150 15 000pg m 5 p 6000pg m0 Oe 500 7X1 5 Ge 60mIiXx1 10 C 100 7X1 pp 100 7 X1 y TMB ee 12m X1 y 147 HSO4 ee 12m X1 y 10XO oo 100m X1 N LO Mouse 1 100 C II 100 Rat Ca 92 TY 4 SC U 1000 2000 3000 mouse C peptide pg ml FNo 631 07231 AKRCP 031 C U 96 65 000 639 07271 AKRCP 030
5. SYBR Gold SYBR Green I EtBr 5 7 35 65 8 120V 8 60 SYBRe Gold Lane1 DGGE Marker I Lane2 DGGE Marker Il SEE QO G G Fragments DGGE Markerl OD DD DGGE Markerl OD D 0 0 65 DGGE Marker lt gt DGGE Marker Il II DGGE Marker Il II DGGE D Code 82 120V 8 60 fragment 53 542 54 55 No
6. 540 8605 1 2 06 6203 1788 103 0023 5 13 03 3270 8243 092 622 1005 082 285 6381 052 772 0788 045 476 2061 029 858 2278 022 222 3072 011 2710285 0720 052 099 0720 0652 806 DM E mail biowin wako chem co jp URL http www wako chem co jp 05 219 Z o1 DN
7. Turbo GFP Phi YFP No 100g 555 81021 AB511 Ant TurboGFP antibody 1004g 30 000 551 81023 AB512 rabbit polyclonal ELISA in cell 2004g 42 000 552 81031 AB513 Anti TurboGFP d antibody 1004g 30 000 558 81033 AB514 rabbit polyclonal 200zg 42 000 ELISA in cell 559 81041 AB603 Anti PhiYFP d antibody OE 1004g 30 000 555 81043 AB604 rabbit polyclonal gt 200zg 42 000 d denature
8. 1 FEPE1L 8 SDS CD 1 4 30 QUO 500 1 35 No W 648
9. TurboGFP C N TurboGFF TurboGFP Gateway destination vector TurboGFP attL1 a ori i ori TurboGFP ateway TurboGFP N Gateway TurboGFP C eritry CIOne 4 3 kb afry Clone 3 kh y entry cione 4 3 kD Kan Kan MCS StOD codon stop cotions FNe 5 557 78271 FP521 Gateway TurboGFP C entry clone 554 78281 FP522 Gateway TurboGFP N entry clone yo Evrogen
10. 1 1 1 zm 1 1mm BCS 001
11. 60 DNase RNase DNase RNase Wako 24 DNase RNase Wako Bio Window DEC 2005 No 72 No TI 078 04981 3 600 074 04983 Gentamicin Sulfate DNase RNase dE 9 800 072 04984 S1 000 070 04941 3 000 072 04945 Glycerol DNase RNase ET 5 000 079 05131 Glycogen Solution abt 20mg m from Mussel DNase RNase 10 500 072 05001 4 500 074 05005 Guanidine Hydrochloride DNase RNase 13 000 078 05003 20 000 073 04992 3 500 075 04991 Guanidine Thiocyanate DNase RNase 7 000 077 04995 25 000
12. EG 750 0 EG 750 0 450 000 500 000 300 000 KN B MELYe 1 eAA4laUe e 5 9 2010 N s Novagen Origami 2 OrigamiTM Origamirw 2 K 12 trxB gor 2 Origamirw Origamirv 2 HRV3C pET47b 50b Origamirw
13. MethylEasy Competitor A Conyentional Lane 1 100ng DNA Lane2 10ng DNA Lane 3 1ng DNA Lane 4 100pg DNA Lane ve DNA mn mi Competitor A Conventional 0 3 4 lt 96 2 3 4 ve ys Conventional 4 Ve 2 Aagarose gel 100pg 100ng DNA DNA MethyIEasyrw PCR MethyIEasyTM DNA Bisulphite Modification Kit 1 25 MethylEasy High Throughput DNA Bisulphite Modification Kit 1 96 amaxa Cell Line Nucleofector C L Kit ion amaxa Nucleofector Device R T V C L
14. 0 45 m 25mm 50 14 000 G K C NmllereN e e C U C C C ELISA C C 0 gb lot i 2 8 0009 6y HDMitp emm mikafmil sm Goel aye intnginikerse 5 lx LR Ss 1 6Um us Aihaya ee Shibsyagi 7 me ee sm er CcC U
15. 318 06411 5 600 HY 314 06413 DGGE Marker I 5 fragments 16 000 312 06414 30 1504 X5 56 000 311 06401 5 600 NR 317 06403 DGGE Marker II 10 fragments 30 150 20 000 315 06404 70 000 No EE EHP 316 90341 3 000 316 06211 28 000 319 06201 35 000 316 06331 20 000 318 06271 25 000 315 06281 32 000 315 90051 EtBrSolution 9 000 Wako Bio Window DEC 2005 No 72 EvaGreen a Biotium EvaGreenTM Real Time PCR qPCR SYBR Green I AEx 4 Em 500nm 525nm EvaGreenTM SYBR Green 1 SYBR9 Green I 3 5 1 DNA PCR 2 4 3 SYBR Green I Real Time PCR SYBR Green I 2 4 SYBR 1x 6000 EvaGreen j SYBRO 5x SYBR Green Ev
16. 54 000 641 08491 384 54 000 G K Wako Bio Window DEC 2005 No 72 23 Wako Ni Agarose Ni 6X Ni IDA S O lt 20 40 mom 06 40 180 zm lt mM kDa WakoPURE system J N 4 DHFR 23kDa ND 8 66 su lane 1 45
17. 750 1200 1200 MM 1chamber 95 1chamber 480 160 X3 URL http www ecogenomics co jpD 0A 25 000 27 500 30 000 BEG EG 12chamber a TK EE Total RNA 6 is
18. C y FEAM lt gt a a Amax nm DNP 348 MCA Trp Abz 06 DABCYL 485 EDANS AMCA 04 QXLTM 490 488 EDANS AMCA 8 QXLTM 520 508 530 FAM ETIC HiLyte FluorTM 488 QXLrw 570 578 Cy3 TAMRA ROX HiLyte Fluorrv 555 6 QXLrv 610 594 628 ROX Texas Red a QXLTM 670 668 Cy5 HiLyte Flour 647 QXLW QXLTM 680 679 Cy5 HiLyte FlourTM 647 23 800 552 80311 acid SE Succinimidyl Ester 22 000 QXL 490 C2 amine 23 800 C2 maleimide 23 800 hydrazide 23 800 48 800 559 80321 acid SE Succinimidy1 Ester 22 000 QXL 520 23 800 C2 maleimide 23 800 hydrazide 23 800 23 800 556 80331 acid SE Succinimidy1 Ester 22 000 QXL 570 C2 amine 23 800 C2 maleimide 23 800 hydrazide 48 800 23 800 553 80341 acid SE Succinimidy1 Ester 22 000 QXLrw 610 C2 amine 23 800 hydrazide 23 800 vinyl sulfone 23 800 23 800 550 80351 acid SE Succinimidy1 Ester 33 000 QXLrw 670 C2 amine 73 800 C2 maleimide 73 800 hydrazide 73 800 48 800 557 80361 acid SE Succinimidyl Ester 65 8
19. Sodium fluoride Sodium orthovanadate Sodium pyrophosphate 8 glycerophosphate BCA Assay Coomassie Plus 2 8 1 m7 100 m A MEK PMEK we PIERCE Biotechnology PERBIO HeLa B MAPK PMAPK Haltrv Phosphatase Inhibitor Cocktail ie q es Haltrw Phosphatase Inhibitor Cocktail X1 Panel A MEK PMEK MEK C STAT3 PSTAT3 FN Panel B MAP PMAP MAP a PanelC STAT3 PSTAT3 STAT3 8 550 81711 76420 Haltrw FNo 79 79931 78410 Haltrw 59 73851 78415 Haltrw EDTA
20. Y 27632 BMP 94 HPLC 0 I N _ NH C nmCH CHs 1 Uehata M et al Nature 389 990 1997 2HCIHzO 2 Sakamoto K et al 7 Pharmacol Sci 92 56 2003 3 Nishimaru K et al 7 Pharmacol Sci 92 424 2003 CisHztNs0 2HCHzO 338 27 CAS 146986 50 7 FN 257 00511 Y 27632 Y 27632 12 000 KN B Pierce HaItTV 4
21. SYBR Gold 200 300 400 500 600 700 Wavelength nm Ames oO oO x oO oO o EtBr SYBRe Gold SYBRe Gold lt gt GelRedrv 10 000 1 10 000 50m7 GelRedrv 10 000 5z7 4C EtBr SYBR Gold GelRedrTM EtBr EtBr lt 12 1 1kb DNA 200ng 100ng 50ng 25ng 1 XTBE EtBr EtBr No
22. 18 24 lac Overnight ExpressrV 0 Rosetta 2 RosettarM CGG Rosettarw 2 AGG AGA AUA CUA CCC GGA CGG tRNA pRARE2 BL21 Overnight ExpressrV HRV3C pET47b 50b argN5 tRNA BL21 Rosetta BL21 Rosetta M XtRNA DE3 DE3 DE3 pLysS DE3 pLysS 1I 1 U L t
23. gor 2 OrigamirM 18 24 FN EEPDYE 559 80701 71344 3 Origamirv 2 Competent Cells 15 400 556 80711 71345 3 Origamirv 2 DE3 Competent Cells 15 400 553 80721 15 400 550 80731 15 400 557 80741 71408 3 Origamirv 2 DE3 Singlesrv Competent Cells 11rxn 50 X11 18 600 554 80751 71409 3 Origamirv 2 DE3 pLysS Singlesrv Competent Cells 11rxn 5047X11 18 600 551 80761 71402 3 RosettarM 2 Competent Cells 0 4m 0 2m X2 15 400 558 80771 71397 3 RosettarM 2 DE3 Competent Cells 0 4m 0 2m X2 15 400 555 80781 71400 3 RosettarM 2 DE3 Singlesrv Competent Cells 11rxn 50 X11 18 600 550 78901 71403 3 RosettarM 2 DE3 pLysS Competent Cells 0 4m 0 2m X2 15 400 552 80791 71404 3 RosettarM 2 DE3 pLacI Competent Cells 0 4m 0 2m X2 15 400 555 80801 71350 3 Rosetta gamirM 2 Competent Cells 0 4m 0 2m X2 15 400 552 80811 71351 3 Rosetta gamirM 2 DE3 Competent Cells 0 4m 0 2m X2 15 400 559 80821 71353 3 Rosetta gami
24. gt GelRedrv10 000 Buffer 3 300 3XGelRed Buffer50m GelRedrv10 000 15 7 3XGelRedrw 30 3XGelRedrw 2 3 EtBr SYBR Gold GelRed SYBR Gold SYBR Gold 2 3 SYBR Gold 2 gt 1 SYBRS Gold 559 78731 41000 GelRedrw Nucleic Acid Gel Stain 10 000x in DMF Evrogen EVRr GEN Gateway TurboGFP Gateway TurboGFP C N Gateway Technology attL1 attL2 TurboGFP
25. DNA TISSUE DNA 3 RNA 2 DNA PCR RNA RT PCR DNA 6 8 200L 1X10 HL60 rw _ DNA RNA RNA 13 8 p53 exon6 actin mRNA DNA 13 8 PCR NR WS o RNA 13 8 M 100bp ladder pe 1 DNA 10ng L M 100bp ladder 6 8 2 DNA 1ng zL 1 total RNA 100pg zL QuickGene 800 3 DNA 0
26. 307 09241 60315 HS 100 40 000 30 0 100 m 302 09291 60311 250 ATP 250 31 000 ATP 300 09231 60312 250 250 37 500 ATP ATP ATP 304 09251 60254 ATP a 250 25 000 ee 301 09261 60260 ATP ATP 2X10 6M 20 5 000 5 5 301 13431 60263 CT150 RNN 150 40 000 309 09321 60259 AT100 100 45 000 303 09221 60375 II i ND I 100 27 000 HTM o G K ae nippi
27. C U 96 75 000 No 637 07191 S 62 000 636 07281 IAKRIN 011S S 96 62 000 S 5 G T C 100N ATP C 100 10RLU 25 10 4 412X55mm 0 lt 77 Na NN ey
28. EN 010 19531 10 500 019 20231 4 2 Aminoethyl benzenesulfonyl Fluoride mg lt 8 000 015 20233 Hydrochloride DNase RNase 1g 57 000 019 20091 3 600 011 20095 4 Amino 2 hydroxymethyl 1 3 propanediol IDNase RNase 8 600 015 20093 Tris 12 000 014 20482 1 700 018 20485 Ammonium Acetate DNase RNase 5 000 016 20481 9 000 014 20161 3 300 010 20163 Ampicilin Sodium DNase RNase 4 600 012 20162 9 500 022 15371 2 500 028 15373 Benzylsulfonyl Fluoride DNase RNase 6 300 020 15372 25g D 20 000 023 15041 5 Bromo 4 chloro 3 indolyl 100mg 8 000 029 15043 np DNase RNase 9 30 000 025 15361 5 Bromo d chloro indoly D glucuronide 6 100 01590 Leoheglammemem Ss CDNase Race EN 0 0 026 15151 5 Bromo 4 chloro 3 indolylphosphate 5 700 022 15153 Toluidine Salt BCIP p Toluidine Salt DNase RNase 2 16 000 eeeF 033 19682 4 600 035 19681 Cesium Chloride DNase RNase 13 800 037 19685 48 000 032 19451 2 400 030 19452 Chloramphenicol DNase RNase 3 900 038 19453 100g 13 000 030 18911 Cytidine 5 Triphosphate Tetrasodium Solution DNase RNase 10 500 548 02331 Deoxyribonuclease 1
29. 10X Buffer PR 0 0 0 0 0 GE 0 oul 50m 7 MgCl a ee ee 0 a Ge I 62 2 2 dNTP 2mol 0 0 0 te Sal 20 X EvalGreen EE EE EE 0 i EE 87828980CSPSi82S20SES288086c 2 5 7g7 DNA polymerase Hot Start Tag DNA polymerase ee 1 5 units ii 0 1 1 amo1 ee 50 Wako Bio Window DEC 2005 No 72 EtBr SYBR Gold GelRed W Nucleic Acid Gel Stain EtBr Birotium SYBR Gold EtBr 20C AEx AEm 295nm 595nm 20 GelRedrvw 10 000 0 5m 100 30 GelRed Ps
30. 4 9 RLU M ATP ATP ee U pe a CELL 5 4 8 2 NS 232 AC C 100n AC 185X110X75mm 700g 4X10 1X10 10mol ATP Bacillus circulans ATP 10 10 10 10F 10 106 107 109 Cell CFU m No 307 09361 60481 C100x 550 000 300 09351 60183 3 5m C 100N 1000 12 000 ATP ATP
31. glyceraldehyde 3 phosphate dehydrogenase GAPDH alpha fetoprotein AFP PCR lt Tester cDNA gt HepG2 Total RNA 100ng SMART PCR cDNA Synthesis Kit 5 3 dT PCR tag Primer 5 GCATCACTGATCGCACAGGTCGTCTACTTTTTTTTTTTTTTTTTTVN 3 47mer Primer SMART PCR cDNA Synthesis Kit PCR Primer II 5 pGCATCACTGATCGCACAGGTC 3 5 21mer Primer lt Driver cDNA gt Total RNA 100ng SMART PCR cDNA Synthesis Kit Rsa 1 GT AC Tester cDNA 1ng Driver cDNA 200ng PCR HepG2 cDNA NormalLiver cDNA Subtracted cDNA lt GAPDH ET PCR fg HepG2 cDNA 3677 09 We Subtract
32. 075 04871 Guanosine 5 Triphosphate Tetrasodium Solution DNase RNase 10 500 090 05141 Isopropyl D thiogalactopyranoside 6 2 500 096 05143 IPTG o 9 000 121 05242 2 100 123 05241 Lithium Chloride DNase RNase 4 500 129 05243 11 000 126 05111 10me 9 000 122 05113 _ 8 17 000 120 05114 D Luciferin Potassium Salt DNase RNase 51 000 126 05116 131 15052 2 000 NEW 133 15051 Magnesium Chloride Hexahydrate DNase RNase 2 500 135 15055 4 000 131 14572 lt 25m 2500 133 14571 2 Mercaptoethanol 99 DNase RNase 5 100 162 21313 5g 3 300 164 21312 Polyoxyethylene 20 Cetyl Ether Brii 58 DNase RNase 26g 5 700 EA Polyoxyethylene 23 Lauryl Ether Brij 35 DNase RNase 166 21615 163 21201 Polyoxyethylene 10 Octylphenyl Ether 4 3 700 165 21205 Triton X 100 DNase RNase 560my 9300 160 21211 Polyoxyethylene 20 Sorbitan Monolaurate 2 200 166 21213 Tween 20 DNase RNase 3 000 161 21621 Polyoxyethylene 20 Sorbitan Monooleate 3 a 50m 2 000 163 21625 me Sm Me re macht 000 164 21552 2 300 166 21551 Potassium Acetate DNase RNase 3 100 168 21555 4 700 166 22112 1 500 NEW 160 22115 Potassium Chlor
33. 1 4 BCS 001 Ne 305 72291 BCS 001 62 000 000 M N 12 7 10 amp 12 13 15 Ecogenomics Inc EG EG EG 750
34. Acids Leukotrienes Lipids Neurochemistry Nitric Oxide Wako Bio Window Oxylipin E mail biowin wako chem co jp Prostaglandin F A X 06 6201 5964 Thromboxane U TN Wako Bio Window DEC 2005 No 72 O A O MOLECUAR TECHNOLOGIES INC DOJINDO Labeling Kits DOJINDO Labeling Kits Dojindo Labeling Kits 2005 10 20 50 Peroxidase Labeling Kit NH 1 sample 6 800 Biotin Labeling Kit NH 1 sample 4 800 Fluorescein Labeling Kit NH 1 sample 8 400 No 344 90823 Peroxidase Labeling Kit
35. NHL 1sample 6 800 343 90893 Biotin Labeling Kit NHL 1sample 4800 343 90913 Fluorescein LabeHng Kit NHz 1 sample 8 400 3sample G T y Buy 2 Get 1 Free 2 1 gt 2005 11 1 2006 2 20 gt y HT No 368 00051 4192 HT 0 24m 25mm 50 14 000 367 00021 4184 HT 0 45zm 25mm 50 14 000 361 00301 4612 0 24m 25mm 50 14 000 368 00311 4614
36. lane 2 lane 3 DHFR a lane 4 Ni Agarose 6X WakoPURE MEF 100K L_ HEFR 20 14 4 No 145 07981 5ml 19 000 141 07983 Ni Agarose 10m 27 000 149 07984 100m 120 000 No 299 59501 WakoPURE system in vitro 4 16 000 295 59503 16 49 800 233 02233 WakoPURE ME 100K 100K 4 4 000 237 02231 WakoPURE system 201 13 000 294 02241 WakoPURE Spin Empty Column 20 WakoPURE system 291 50301 10 9 000 LK
37. 00 QXL 680 48 800 C2 maleimide 48 800 hydrazide 48 800 Wako Bio Window DEC 2005 No 72 AnaSpec FRET No 23884 DABCYL acid Ultrapure Grade 7 500 554 80371 81801 DABCYL acid SE Succinimidyl Ester 16 300 551 80381 81802 DABCYL C2 maleimide 22 000 558 80391 81822 DNP C2 maleimide 12 000 551 80401 23887 EDANS 12 000 FRET No EE 558 80411 23486 01 22 000 23486 Boc Asp EDANS OH 137 500 23487 5g 550 000 8 555 80421 22 000 Fmoc Asp EDANS OH 137 500 550 000 552 80431 22 000 Boc Glu EDANS OH 137 500 550 000 559 80441 22 000 Fmoc Glu EDANS OH 137 500 550 000 556 80451 22 000 Boc Lys DABCYL OH 137 500 550 000 553 80461 23496 01 22 000 Fmoc Lys DABCYL OH 137 500 550 000 U TN Cayman Chemical wm 2006 2007 Cayman Cayman Chemical Cyclooxygenases Cytokines Fatty
38. 05 No 72 5 FUJIFILM QuickGene QuickGene DNA RNA QuickGene 800 QuickGene 610L QuickGene 800 ann 8 6 DNA DNA RNA
39. 07 oe tt p 19 DMT DOJINDO Labeling Kits p 20 Buy 2 Get 1 Free p 20 p 23 Total RNA Wako DsDD cDNA Subtraction Kit WMako DsDD cDNA Subtracton cDNA cDNA Total RNA SMART Total RNA HepG2 ATCC No HB 8065 Normal Liver BioChain Total RNA 100ng SMART PCR cDNA Synthesis Kit cDNA Tester cDNA HepG2 Driver cDNA Normal Liver DsDD cDNA Subtractton Subtracted cDNA HepG2 cDNA Normal Liver cDNA Subtracted cDNA 10ng
40. 08261 NCO430639 25cm2 60 30 000 645 08271 NCO430641 75cm2 10 9 000 642 08281 150cm2 19 000 649 08291 175cm2 20 000 642 08301 225cm2 22 000 649 08311 100mm 10 500 646 08321 150mm 12 000 643 08331 8 40 000 640 08341 14000 647 08351 18 600 644 08361 19 000 641 08371 22 000 648 08381 23 000 645 08391 28 000 648 08401 96 28 000 645 08411 96 40 000 642 08421 96 40 000 646 08941 25 000 649 08431 96 46 000 646 08441 96 46 000 643 08451 36 000 640 08461 37 000 647 08471 384 37 000 644 08481 384
41. 1ng pL 2 total RNA 10pg zL Wako Bio Window DEC 2005 No 72 Ne 636 05601 QuickGene 800 1040000 633 05611 33 400 630 05621 QuickGene RNA S 51 400 637 05631 37 400 638 07001 51400 639 07151 26 500 QuickGene 610L 2m7 50 7g DNA na QuickGene 800 200 7 QuickGene 610L 2m 10 DNA DNA 12 6
42. BIO VVINAOW DsDD cDNA Subtraction Kit Wako o li D 2 DGGE Marker 1 DGGE Marker p 3 Evrogen Gateway TurboGFP p 6 Evrogen p 6 Human Genetic Signatures MethyIEasy DNA Bisulphite Modification Kit D 7 amaxa CellLine NucleofectorBC L Kit p 8 EG Roeser p 15 Biotium _EvaGreenM 5 oo p 4 Biotium GelRed Nucleic Acid Gel Stain p 5 AnaSpec QXLTM p 18 Pierce Halt p 9 Novagen os i iis p 16 Ni Agarose DE EE RE RT ER p 24 Y 27632 Y 27632 ee D 8 FUJIFILM QuickGene ES DE CRE D 12 Cybox p 14 C 1 00 D 22 HP p 10 p 14 Cayman 2006 20
43. Bovine recombinant RNase Prot 1 000U 7 000 544 02333 Solution RNase free Protease free ON 30 000 041 29231 adenosine 5 Triphosphate Sodium Salt Solution DN 7 900 048 29241 cytdine 5 Triphosphate Sodium Salt Solution 7 900 045 29251 guanosine 5 Iriphosphate Sodium Salt Solution 7 900 042 29261 hymidine 5 Triphosphate Sodium Salt Solution 7 900 049 29271 uridine 5 Triphosphate Sodium Salt Solution 7 100 294 60801 5 Iriphosphate Sodium Salt Solution Set D 18 000 043 29291 Deoxyribonucleoside 5 Triphosphate Sodium Salt Mixture Solution DNase RN 9 000 045 29192 1 700 047 29191 WW Dimethylformamide DNase RNase 2 000 049 29195 3 400 041 29351 50m 2 600 047 29353 Dimethyl Sulfoxide DNase RNase 3 900 043 29355 9 900 044 29221 2 300 Dithiothreitol DNase RNase 10 000 042 29222 34 000 2 0 079 05011 3 500 075 05013 D Galactosamine Hydrochloride DNase RNase 7 400 073 05014 25 000 0749 SOmg m Genetdn Disulate Solutton DNase RNase 4 100 59000 DNase RNase Protease
44. RNA M I U I U M I U I DU Human tissue plasminogen eulWtRNA activator pET 21d 2 metTtRNA S 4965 bp pLysSRARE2 7667 bp mm lt argW tRNA am thrT tRNA PE gy7 tRNA yrUtRNA thrU tRNA argU tRNA 6591 bp Rosetta gami 2 Origamir 2 RosettarM 2 Rosetta gamirv 2 Origamirv 2 pRARE2 recA endA acT K 12 HS DXidiZed i HS argX tRNA yo tRNA leuW tRNA SH 1 argWw tRNA gbyTtRNA argU tRNA 1 ileX tRNA SH Target Protein Target Protein L 7 tRNA trxB
45. aGreen 1x 5000 EvaGreen 0 5x 4000 3000 2000 1000 PCR PCR lt 1 gt Real Time PCR 2 Real Time PCR PCR SYBR Green I PCR SYBR Green I SYBR Green 1 PCR 0 SYBR Green 100 LL so 0 60 extra peak 40 20 5 O 0 0 5 10 15 20 25 30 35 40 PCR lt 3 gt EvaGreenrw 4 gt PCR DNA 96C PCR 4 SYBR Green I PCR 1 TBP 2 SDHA 3 RPL4 4 HMBS Real Time PCR gt 50
46. d d LF DNA 5 3 HUMAN GENETIC SIGNATURES Tk MethyIEasy V DNA Bisulphite Modification Kit bisulphite DNA DNA 5 5 mC 5 mC DNA PCR DNA DNA Methylation Specifc PCR OBRA MS SNuPE MALDI TOF DNA DNA NN DNA 1 100p
47. ed Normal Lver EE cDNA Subtracted cDNA 0 31 15 Hep G2 N Liver Subtracted AFP gt AFP PCR PCR Subtracted cDNA HenG2 cDNA 12 27 4097 Hep G2 GAPDH i _ Normal Liver HepG2 AFP 20 51 15 28 CDNA Subtracted UNMI14 1251 1934 86 ko Hep G2 N Liver Subtracted 294 62001 DsDD cDNA Subtraction Kit Wako Wako Bio Window DEC 2005 No 72 DGGE DNA DGGE Marker1l 5 fragnnents DGGE Marker ll 10 fragments DGGE denaturing gradient gel electrophoresis DNA DGGE Marker1 50ng 5 ul DGGE DGGE Markerll 100ng 5 pl DGGE 10mwTris HCI pH8 0 10m EDTA pH8 0 0 025 Bromophenol Blue 35 Glycerol DGGE 2oc 35 IIH 1 5z Loading Buffer
48. g DNA 2 Cytosine one DNA DNA 7 Deaminatr DNA 1 A 96 T oe 1 20 25 i We 1 gt MethyIEasyrv DNA Lane 1 MethylEasy DNA Reagent mene 5 2mIXx 1 3 Reagent 9 earner 2gX 1 x 4 NN 2 Reagent 3 ree 3mIx 1 Reagent A ee 25mIx 1 Control Sample 1 1 DNA 40 7X1 Control Sample 2 2 DNA 20 7X1 Control Samples 3 3A amp 3B 40 7X2 p Microcentrifuge tubes or 2m7 X25 2 Aagarose gel 1 8 29 DNA MethyIEasy 2 PCR20 DNA 3PCR20 DNA 2 gt DNA MethyIEasyrw
49. ide DNase RNase 2 500 168 22111 4 000 166 21671 100m7 2 600 168 21675 2 Propanol DNase RNase 3 600 166 21051 14 700 191 13912 1 700 NEW 195 13915 Sodium Acetate DNase RNase 2 500 193 13911 4 000 192 13925 2 000 NEW 190 13921 Sodium Chloride DNase RNase 1kg 3 500 198 13721 3 000 196 13792 Sodium Cholate DNase RNase 35 5 500 194 13402 10 000 196 13401 Sodium Dextran Sulfate 5000 DNase RNase 23 000 198 13405 105 000 190 13982 2 500 DY 192 13981 Sodium Dodecyl Sulfate DNase RNase 3 800 194 13985 12 000 191 13831 rm 1g 4300 NEW 197 13833 Spermidine DNase RNase 13 000 198 13525 1 500g 3 500 196 13521 Sucrose Ultra Pure DNase RNase 5 200 204 16212 1 500 NEW 206 16211 100w v Trichloroacetic Acid Solution DNase RNase 2 800 208 16215 8 800 215 01211 3 400 217 01215 Urea DNase RNase 6 000 211 01213 9 000 212 01101 Uridine 5 Triphosphate Tetrasodium Solution DNase RNase 10 500 DNase RNase K O Wako Bio Window DEC 20
50. rM 2 DE3 pLacl Competent Cells 0 4m 0 2m X2 15 400 556 80831 71352 3 Rosetta gamirM 2 DE3 pLysS Competent Cells 0 4m 0 2m X2 15 400 DE3 DE3 pLysS 3 0 4m No 553 80841 71431 3 Origamirv 2 Competent Cell Set 1set 0 4m X3 551 78811 71405 3 Rosettarv 2 Competent Cell Set 1set 0 4m X3 559 78851 71432 3 Rosetta gamirM 2 Competent Cell Set 1set 0 4m X3 Wako Bio Window DEC 2005 No 72 QXL ANSSPEC FRET Fluorescence Resonance Energy Transfer FRET FBET RE dr W AnaSpec FRET QXLrw
Download Pdf Manuals
Related Search