pBAsi-hH1 DNA
Contents
1. Adenovirus Dual Expression Kit 6170 pBAsi 3 DBAsi hH1 DNA 3220 human H1 promoter Accession No S68670 pBAsi hU6 DNA 3221 human U6 promoter Accession No X07425 pBAsi mU6 DNA 3222 mouse U6 promoter Accession No X06980 pBAsi hH1 DNA EcoRV Cl promoter human H1 BamH Xba Sal Acc Hinc Il Pst Sse8387 Sph Hind Ill EcoRV pBAsi hH1 2 790 bp Cla Amp 10mM Tris HCI pH8 0 1mM EDTA HR 2 790bp 1 dideoxy 2 PamH EcoRI 1 APA Cal EcoRV 2 1 Brummelkamp et al 2002 Science 296 550 553 2 Lee etal 2002 Nat Biotech 20 500 505 3 Paddison etal 2002 Genes and D2. ents described on the product web page It is your responsibility to review understand and adhere to any restrictions imposed by such statements All trademarks are the property of their respective owners Certain trademarks may not be registered in all jurisdictions v201401 pBAsi hH1 DNA Code No 3220 Size 20 ug Shipping at 20 C Store at 20 C 2 Lot No pBAsi RNA polymerase Ill pol Ill siRNA pol lll RNA DNA siRNA RNAi RNA
3. ev 16 948 958 4 Paul erg 2002 Nat Biotech 20 505 508 5 Suierg 2002 Proc Natl Acad Sci USA 99 5515 5520 6 Kawasaki et g 2003 Nucleic Acids Res 31 700 707 7 Tuschl T etal 2003 Nat Biotech 20 446 44 8 Shen C et al 2003 FEBS Lett 537 111 114 sier prea v201401 077 543 6116 TaKaRa ri 077 543 1977
4. pBAsi hH1 DNA Code No 3220 Size 20 ug Shipping at 20 C Store at 20 C x 2 years from date of receipt under proper storage conditions Lot No Concentration 9g ul Volume ul Regarding protocol please refer to the product manual supplied with this vector Description pBAsi vector Series is a plasmid vector containing a RNA polymerase Ill pol Ill promoter for expression of siRNA By inserting synthesized oligo DNA sequence for hairpin RNA into the downstream of the pol Ill pro moter siRNA expression vector is constructed The obtained plasmid is transfected into cells to perform RNAi experiment Moreover the region composed of the promoter and hairpin RNA se quence can be excised and be easily put on adenovirus vector plasmid for the construction of siRNA expression recombinant adenovirus Adenovirus Dual Expression Kit Cat 6170 can be used for this purpose Three kinds of plasmid vectors are available in pBAsi vector series Each carries each defferent promoter A customer can choose an appropriate vector depending on a kind of target cell and an experimental purpose pBAsi hH1 DNA Cat 3220 human H1 promoter Accession No S68670 pBAsi hU6 DNA Cat 3221 human U6 promoter Accession No X07425 pBAsi mU6 DNA Cat 3222 mouse U6 promoter Accession No X06980 Restriction enzyme map EcoRV Clal promoter human H1 BamH Xba Sal Acc Hinc Il Pst S
5. se8387 Sph Hind Ill EcoRV pBAsi hH1 2 790 bp Cla Amp Form 10mM Tris HCI pH8 0 1mM EDTA Preparation Purified by ion exchange column treatment Chain length 2 790 bp Purity Confirmed to maintain promoter and cloning site by dideoxy sequenc ing method Confirmed to be cleaved at a single site by restriction enzymes BamH and EcoRI and at two sites by restriction enzymes C a and EcoRV References 1 Brummelkamp et al 2002 Science 296 550 553 2 Lee etal 2002 Nat Biotech 20 500 505 3 Paddison etal 2002 Genes and Dev 16 948 958 4 Paul et g 2002 Nat Biotech 20 505 508 Sui etal 2002 Proc Natl Acad Sci USA 99 5515 5520 Kawasaki et a 2003 Nucleic Acids Res 31 700 707 Tuschl T etal 2003 Nat Biotech 20 446 44 8 Shen C et al 2003 FEBS Lett 537 111 114 5 6 7 Note This product is for research use only It is not intended for use in therapeutic or diagnostic procedures for humans or animals Also do not use this product as food cosmetic or household item etc Takara products may not be resold or transferred modified for resale or transfer or used to manufacture commercial products without written approval from TAKARA BIO INC If you require licenses for other use please contact us by phone at 81 77 543 7247 or from our website at www takara bio com Your use of this product is also subject to compliance with any applicable licensing requirem
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