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1. 121C 1 2 o gt 1 2 8C 2 0 18
2. 8G 4 30C 3 2 8C 2 8C a 1 10 28C 5 15 25C 8 2 8C 9 1 2 8C 1 18 25C 1 2 8C 4 ELISA BEP m 2 3 BEP 450 1
3. 2 1 log MIUL A9 a 8B 10 E 25 E HBs HBs 00 25 1 9 1 10 WHO 2 IU L
4. HIV 1 H 1 V 2 1 6 1
5. 2 EDTA 1 96 x 1 Anti HBs II MTP HCV HAV EE HBs 2 6mLX1 Anti HBs Conj POD HBs 3 1 5mL x 1 56C 30 Anti HBs IIREFP
6. 2 1 5 8 CV 9 P1 1 25 4 0 P2 1 65 3 2 P3 0 59 7 7 P4 0 28 3 7 CV 9 P1 1 25 3 9 P2 1 65 3 2 P3 0 59 8 3 P4 0 28 12 7 5 WHO Reference Standard 1 HIV HBV HCV 3 SS FA TE EU Vito Diagnostic Directive in the ae HIV 1 HIV 2 HBV HCV
7. 5 690nm 3 m 650nm 61 H 37 1C 1H 6 25uL 100uL 4 Al D1 100uL 1 E1 100uL 100uL 1
8. HBs B HBs HBs 3 4 d International Standard 2008 NBSC code 07 164 7 B HBs 9096 HBs B HBs HBs HBs H 1 B 2 93 f Bs B HBs 1096 HBs
9. 18 18 18 18 24 24 22 60 96 1 Tiollais P Pourcel C Dejean A The hepatitis B virus Nature 1985 317 489 95 2 ww 0 gt Lau JY Wright TL 342 1335 40 FrOsner G Viral he Molecular virology and pathogenesis of hepatitis B Lancet 1993 patitis In Thomas L ed Clinical Laboratory Diagnostics Frankfurt Main TH Books 1998 1260 87 Robson SC Scho 1994 84 530 5 Deinhardt F Zucke onViral Hepatitis i Jilg W Schmidt Bvaccine J Med Vi Ferguson M Heath replacement for the World Health Orgal b B Abdool Karim SS Viral hepatitis B an overview S Afr Med J man AJ Immunization aainst hepatitis B Report on a WHO Meeting Europe J Med Virol 1985 17 209 17 Deinhardt F Immune resDonses to late booster doses of Hepatitis 0 1985 17 249 54 A Report of the WHO collaborative study to calibrate a candidate First International Standard for hepatitis B immunoglobulin Geneva ization 2008 WHO BS 08 2084 1 22
10. 37 1 60 2 earnerp 5 03mL 4 2 i 20 6 100uL 7 8 15 25C 30 2
11. TEL 03 3493 8400 1 1 1 1 DL 114 005E 0QNE G15 C0502
12. 9 1 450nm 690nm BEP 6 100uL 650nm 615 BEP 5 1 3 H 6 EP BEP BEP B BEP
13. CV1 1 1596 E 755 HBs 99 1969 b HBs 216 215 HBV 539 533 WHO reference preparation 81IU 824 99 896 HB 1 R HCV HBs
14. B 8 E 1H al 3 HIV HBV HCV
15. HBs 4 14mL x 2 Anti HBs IINeg 1 5 100mL x 1 15 25C Washing Solution POD Concentrate 15 25C 6 30mL X1 Buffer Substrate TMB 7 3mL 1 Chromogen TMB 1 20mL 400mL 8 100mL 1 1 Stopping Solution POD 2 1mL 1
16. 2 1H 30 25uL 4x100L 1x100uL 100uL 1x100uL 60 2 37 1C y 4 h 4 100uL 30 2 15 25C 8 1 450nm 650nm 100uL
17. G Xa 3 5 2
18. ELISA HBs 100 000 IU L 1 999 1 99 20uL 180L sad 3 RR 2 8C HBs a HB HBs a HBs Es WeAnceayty2 jp 2 8C 4
19. 2 BEP BEP A ns 0 08 i nBs lneg 0 08 As As E O 1
20. BEP MI 1 Hg Ne Agrmgrp 0010 SA 0 120 Nings REFP 07 1 4 1 3 2 BEP Inva 8 id Test ee 2
21. 1 1000AMYO0051000 1 EDTA EDTA 2 8C 8 15 25C H 2 8C 3
22. H 1 HBs 8 H 80 12096 E HBs HBs mg Recommendations e Robert Koch Institute in Ger 1 any October 0 120 0 700 Bs 3 1 3 2 HBs E HBs 3
23. 0mL 1 6 1 1 1 2 10 IUL 100 UL HBs HBs 100 UL 1000 UL 1 9 20pL 180uL 10 000 1U L 1 99 1 9 20uL 180uL
24. B H 2 an HBs I 1 100 of the Standi 1995 HBs HBs gt 10 HBs 5 UL 0 WHO UL E f 9 Committee on Im UniZation oft 1 2 3 HBs 4
25. OQNE SIEMENS 2013 4 2 008 10 1 B Anti HBs II Enzygnost Anti HBs 1l H HH

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