SPRIworksフラグメントライブラリシステム
Contents
1. II GS FLX 2 1 350bp 500bp 6 DN A 2 GS FLX 350bp 00 jk Consistent and Reproducible Library Yield of Raw Wells 1 087 101 1 064 381 Oneweay Analysis of Wean Tetal Molecules By Test of Filter Pass Wells 772 005 751 678 Filter Pass 72 83 72 77 pct_mixed failed SISSY 3 53 Dct_trimmed_too_short_primer 0 79 0 55 read_length_stdev 110 76 120 42 Reads Assembled Passing 92 55 90 58 Reads Assembled 714k 681k 7 SYBR FAST qPCR KAPA assembled length 4 5Mb 4 5Mb SPRIworks I SOLiID2. SPRI TE SPRI Solid Phase Reversible Immobilization an DNA SPRI TE ss 1 SPRIworks SPRIworks SPRI 10 4 5 3 SPRI SPRIworks 3 3 SPRIworks
3. 1 4 DNA 150 250bp 150 350bp 3 SOLiD 1uqg DNA SPRIworks DNA 8 SPRIworks qPCR 9 150 250bp 2 4ng 150 350bp 19 23ng 20 1 1ng SPRIworks
4. 1 H SPRLTE 3 1 SPRIworks SPRIworks Sa Es SPRI TE SPRlworks DNA PCR DNA 4 5 PCR PCR PCR DNA
5. a 3 A VE AN SE I a 20 2 eo2 0 SPRHTE DNA lt c SM 2 120 2 START SRPRIwoiks 1 3 DNA 10 5 SPRIworks I Genome Analyzer HiSeq 1 1 5
6. SOLiD4 3 1 150 250bp 150 350bp 1 BE SE 8 DNA 3 SOLiD4 Sequencing Metric Sequencing Metric normalized reads per NE of Mappable unigue start point Reads SOLiD V 4 0 Post library metric Library Type Enrichment SPRIworks multiplex gdapters 150 350 21 8 3 06 392 21 742 905 Size Selection SPRIworks regular gdapterS no SiZe 218 2 81 16 8 19 816 994 Selection Size Selection within Nick rans Manual regular adapters gel size 14 6 SN 27 6 22 603 855 Selection 9 SYBR FAST qPCR KAPA SPRIworks gt SPRIworks
7. 1 A88267 SPRIworks 1 1 4 550 000 A88267 SPRIworks TI 2 4 550 000 Genome Analyzer HISeqd ST A98473 SPRIworks 3 4 550 000 I SPRIworks GS a GS Junior Genome Analyzer HiSeq a a gt A84801 SPRIworks I 10 138 000 SOLiD A84803 SPRIworks I 50 650 000 GS FLX GS Junior 1 10 A84805 SPRIworks TI 10 128 000 1 4 5 A84806 SPRIworks TII 50 550 000 5 SR gm SOLiD A98476 SPRIworks I 10
8. A85410 SPRIworks II 3 SOLID A50100 SPRI TE A98476 SPRIworks 10 A98475 SPRIworks SPRI SPRI TE Beckman Coulter Beckman Coulter Beckman Coulter Inc lt SS BECKMAN COULTER 135 0063 3 5 7 TOC We re beffer fogefther 0120 566 730 http www beckmancoulter co jp B90023 2011 07 4000 E
9. 1 DNA 200 400bp 300 600bp 3 10 SPRIworks DNA 5 SPRIworks SPRIworks 1 GA 1 1 MiSeq 2 200 400bp 300 600bp 5 DN A 1 GA Sample name Read length Total Sequence Sequence Mapped WI
10. 128 000 SPRIworkS 500 W x612 D xX645 H mm Genome Analyzer HiSeq A84868 SPRIworks 1 500 000 6 Kg GS FLX GS Junior A85410 SPRIworks II 500 000 SOLID A98475 SPRIworks 500 000 1 7e ak N 6 A8487 1 SPRIworks Nebulizer 10 80 000 A84872 SPRIworks Nebulizer 50 365 000 SPRIworks A84879 SPRIworks 1 10 45 000 A50094 SPRI TE 450 000 1 Genome Analyzer HiSeq A50100 SPRI TE A84801 SPRIworks 1 10 A84868 SPRIworks T 2 GS FLX GS Junior A50100 SPRI TE A84805 SPRIworks II 10
11. DNA DNA DNA DNA cDNA PCR 2 SPRIworks 10 SPRIworks 1 10 8 10 5 S 3 9 3 vs SPRIworks I 1 20 4 Number of Libraries 10 5
12. 2 COULTER We re better fogether SPRIworks 5 EEE 1 DNA DNA DNA SPRIworks
13. al 6 9 z16 6 lt 9 Reads per Startpoint avg SPRIworks shot 8 36 17 162 237 13 988 940 12 491 985 2 09 size selection Control PhiX 36 25 040 301 22 092 804 20 283 538 1894 06 SPRIworks Iong 51 28 273 819 21 431 987 18 237 102 2 82 size selection SPP o rSs 7 GS FLX GS Junior IT 1 4 II DNA 350bp 500bp 3 GS FLX Titanium Rapid Library MID 500ng DNA SPRIworks DNA 6 SPRIworks KAPA SYBR FAST qPCR 7
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