Retrovirus Titer Set（for Real Time PCR）
Contents
1. RNA http wwwr takara bio coJp 4 6166 RT PCR 2 1 RNA Control Template2. http wwwr takara bio coJp 11 6166 TafdR4 Tel 077 543 6116 Fax 077 543 1977 http www takara bio coJp v201303Da
3. http wwwr takara bio coJp 3 6166 V One Step SYBR PrimeScript RT PCR Kit Perfect Real Time 1 co transfection RT PCR DNase DNase DNA
4. MOI multiplicity of infection MOI RNA 4 MOI One Step SYBR PrimeScript RT PCR Kit Perfect Real Time SYBR Green 1 RT PCR cDNA PrimeScript PCR
5. 1 2 3 4 Master Mix RNase Free dH2O Master Mix One Step SYBR PrimeScript RT PCR Kit Perfect Real Time TakaRa Ex 7ag HS PrimeScript RT enzyme Mix I 50 20 20 One Step SYBR PrimeScript RT PCR Kit Perfect Real Time 2XOne Step SYBR RT PCR Buffer Vortex
6. DNase 1 1 gt 12 5 ul 10 X DNase Buffer 2 5 ul DNasel 5 U ul 2 0 wl RNase Inhibitor 40 U wl 0 5 ul RNase Free dH20 7 5 ul Total 25 ul 2 DNase 37 30 70 10 20 DNase Recombinant DNase RNase free 2270A 10 Xx DNase1Buffer Ricombinant RNase Inhibitor 2313A 2 2 RNA Control Template RNA Control Template RT PCR RNA RNA
7. RNA Control Template 1 EASY Dilution for Real Time PCR RNA Control Template 5 X108 copies 5 1 x 108 copies u 1 2 EASY Dilution for Real Time PCR 18 ul 2 6 3 1 1 Xx 108 copies uu 2 ul 2 4 2 2 u 3 5 6 RNA ul EASYDilution ul copies u 8 RT PCR RNA Control Template 2 2
8. 2 4 29 y 1 0036x 27 763 RSq 0 9983 25 Eff 99 5 23 Ct 15 t 1 1 82 18 2 182 1820 x10 ivp ml 4 http www takara bio coJp 9 6166 VII Appendix RNA Control Template RNA Control Template RNase RNA RNase RNA VII PCR Thermal Cycler Dice Real Time System TP900 Thermal Cycler Dice Real Time System Lite TP700 8 amp 0 2 ml Hi 8 Tube 0 2 ml Hi 8 Flat Cap
9. 7g42 Ex Tag HS 1 RT PCR 1 RT PCR SYBRS Molecular Probes Inc 100 25 1 Forward Titer Primer FRT 1 10 pmol ul 50 ul 2 Reverse Titer primer RRT 1 10 pmol ub 50 ul 3 RNA Control Template 5x108 copies ul 10 ul 4 EASY Dilution for Real Time PCR 1 mlX2 5 DNase 1 RNase Free 5 U ul 40 ul 6 10 Xx DNase Buffer 50 wil 7 RNase Inhibitor 40 U wl 10 ul RT PCR 100 DNase 20 20C 20 RNA Control Template Deep freezer 70 80 I One Step SYBR PrimeScript RT PCR Kit Perfect Real Time RR066A One Step SYBR PrimeScript RT PCR Kit Il Perfect Real Time RR086A http wwwr takara bio coJp 2 6166 IV
10. TP900 5 http www takara bio co jp 7 6166 VI 1 1 GT T Retro DMEM 10 FBS 2 4 8 16 32 RT PCR 2 Ct 2 3 25 y 1 0732x 19 042 4 RSsq 0 9908 1 0
11. 61 60 IdfaR4 Retrovirus Titer Set for Real Time PCR vV201303Da Retrovirus Titer Set for Real Time PCR RT PCR MLV murine leukemia virus RNA RNA One Step SYBR PrimeScript RT PCR Kit Perfect Real Time RR066A RR086A RNA 4 MLV MLV RNA
12. 3 One Step SYBR PrimeScript RT PCR Kit Perfect Real Time RR066A 4 DNase V 1 5 VI 1 http wwwr takara bio coJp 6 6166 4 Thermal Cycler Dice Real Time System Thermal Cycler Dice Real Time System Pattern 1 Hold 42 5 95C 10 Pattern 2 PCR Cycle 40 95 5 60 30 Pattern 3 Dissociation 1 Thermal Profile Setup Thermal Cycler Dice Real Time System
13. 908 22 21 20 19 1 1 1 1 2 4 8 16 32 3 DMEM 10 FBS Ct 21 2 y 1 0862x 13 676 RSq 0 9945 Ef 89 3 8 3 7 6 5 4 3 1 1 1 1 2 4 8 16 32 64 2 GT T Retro http www takara bio co jp 8 6166 2 1 DMEM 10 FBS ZsGreen HT1080 FACS 1 82 x 106 ivp ml 10 100 1 000 DNase RT PCR
14. NJ300 NJ302 RetroNectin Recombinant Human Fibronectin Fragment T100A B CultiLife Spin KB630 Retrovirus Packaging Kit Eco Ampho 6160 6161 DNA pDON AL2 Neo DNA pDON Al 2 DNA 3653 3654 pMEI 5 Neo DNA pMEI 5 DNA 3655 3656 siRNA DNA pSINsi hH1 DNA pSINsi hU6 DNA pSINsi mU6 DNA 3660 3661 3662 http wwwr takara bio coJp 10 6166 IX
15. 10 5 VI 2 http wwwr takara bio coJp 5 6166 3 RT PCR 1 gt lt 2 x One Step SYBR RT PCR Buffer IIl 3 12 5 ul 1 Xx TakKaRa Ex Tag HS 5 U ul 3 0 5 PrimeScript RT enzyme Mix ll 3 0 5 ul Forward Titer Primer FRT 1 10 pmol ul 05ul 0 2uM Reverse Titer primer RRT 1 10 pmol ub 05ul 0 2uM 45 2 ul RNase Free dH2O 3 8 5 ul Total 25 ul 1 Thermal Cycler Dice Real Time System TP900 2 1 2
Download Pdf Manuals
Related Search