PrimerArray® Embryonic Stem Cells
Contents
1. SYBR Molecular Probe Inc http wwwr takara bio coJp 15 PH016 PNO016 dfaHRd4 Tal B11G Fax 1 http www takara bio co jp v201209Da2. PrimerArray PrimerArray 2 PrimerArray PCR 4 ul PCR PrimerArray 60 60 60 0 0 60 60 60 6 AA AXAX OOOOOOOOO OOOOOOOOOOOO CC OGKOGK2OKOG RY OCG OOOOOOOOOOOO AXAXAXXAXX XX OOOOOOOOOOO OOOOOOOOOOOO Test Sample http wwwr takara bio coJp 8 PH016 PNO016 3 PCR Sealing Film Plate Sealing Pad Sealing Film PCR Sealing Film
3. Perfect Real Time http www takara bio co jp realtime SYBR Green RT PCR RefSeq VI 2005 3 4 PCR 120 126 2008 PCR 3 1 RT PCR 39 43 2 VII
4. PCR PCR 4 24 4 PrimerArray PrimerArray 5 PCR PCR PCR PCR 1 PCR 2 PCR 1 3 PCR 95 30 2 Step PCR 95 5 60 30 40
5. PCR Thermal Cycler Dice Real Time System TP900 TP960 PCR 8 RNA NucleoSpin RNAII 740955 10 RNAiso Plus 9108 9109 DNase Recombinant DNase 1 RNase free 2270A PrimeScript RT Master Mix Perfect Real Time RR036A PCR SYBR Premix Ex Tag 1 Tli RNaseH Plus RR820A PrimerArray Analysis Tool for Embryonic Stem Cells Multiplate RO http wwwr takara bio coJp 4 PH016 PN016 0 1 1 4 ONI NR
6. Cs 2 RNA RNA IV Appendix 1 RNA 2 1 RNA NucleoSpin RNA II 740955 10 RNAiso Plus 9108 9109 RNA total RNA RNA DNA TE Buffer 250 ng ul 2 2 DNA total RNA DNA DNA PCR DNA total RNA DNase DNA NucleoSpin RNA II 740955 10 RNA
7. 3 24 1 4 15 000 rpm 5 4 10 3 M 250 ul gt 99 10 5 4 15 000 rpm 15 6 70 4 C 15 000 rpm 5 7 8 DEPC http wwwr takara bio coJp 6 PH016 PNO016 3 2 RNA total RNA PrimeScript RT Master Mix Perfect Real Time RR036A 1 1 Xx PrimeScript RT Master Mix 10 ul 1 Xx total RNA 250 ng ul 10 ul 2 5 ug RNase Free dH20 30 ul Total 50 ul
8. PCR 1 110 SYBR Premix Ex Tag Ti RNaseH Plus 2 x 12 5 ul 1 375 ul cDNA 50 ng ul 0 4 ul 44 wl dH2O 8 1 ul 891 Total 21 ul 2310 ul total RNA http wwwr takara bio coJp 7 PH016 PNO016 2 PCR 21 ul 8 1 8 13 273 ul 2 8 PCR 21 ul OOOOS QOQOQQQOQOQQOOQOC DOGOO 8 OO OOOC 8 COOCCOOCCCO COOCCOOCCOOC Control Sample Test Sample 4 2 PCR PrimerArray 1 PrimerArray
9. Threshold 2 Ct Tm Ct Tm PCR 3 PrimerArray Analysis Tool for Embryonic Stem Cells Ct Excel CSV Multiplate RQ Ct Multiplate RQ Thermal Cycler Dice Real Time System Multiplate RQ PCR Omit PrimerArray Analysis Tool for Embryonic Stem Cells h
10. 1E 02 1E 01 1E 00 1E 01 1E 02 1E 03 1E 04 1E 05 1E 05 1E 04 1E 03 1E 02 1E 01 1E 00 1E 01 1E 02 Control Sample Test Sample http wwwr takara bio coJp 10 000 00 100 00 PH016 PNO16 PrimerArray Embryonic Stem Cells 50 ul X 88 50 ulx 8 Forward amp Reverse primer mix 2 5 uM 25 12 Excel http www takara Dio CO jD Drimerarray_tool2 gE 4 5 H 1 11 12 1 one na ena EE EA ETE ET EE EE EE EE EE mlelmlwlmlmlglmlmlalnla al lalalzlalelmlmlelmle Islewlwlmlmlslwlelmlwlmwlw DD
11. 2 5 ug total RNA Y 20 PrimeScript RT Master Mix Perfect Real Time RR036A total RNA cDNA Y PCR 10 cDNA SYBRe Premix Ex Tag 1 Tli RNaseH Plus RR820A PCR Control Sample Test Sample 1 http wwwr takara bio coJp 2 PH016 PNO16 5 8 PrimerArray PCR PrimerArray Y PCR 1 2 Control Sample PCR PCR Control Sample i tt gi am fe Y 30 Test Sample Mi 1 Fett Penh nih 8 m mm wm fat Ct AACt
12. 2 37 15 85 5 4C 4 6 PCR Thermal Cycler Dice Real Time System IV Appendix 2 PCR 4 PCR 1 2 PCR 4 1 3 cDNA SYBR Premix Ex Tag II TIi RNaseH Plus RR820A PCR Control Sample Test Sample 1 cDNA cDNA
13. http wwwr takara bio coJp 9 PH016 PNO016 6 PCR PCR PrimerArray Analysis Tool for Embryonic Stem Cells Multiplate RQ 1 PCR a en mmm gs mm E er na Threshold PCR Log scale
14. DNase RNA DNase http wwwr takara bio coJp 5 PH016 PNO016 DNase DNA total RNA Recombinant DNase RNase free 2270A DNA DNase 1 total RNA x ul 20 50 ug 10 x DNase Buffer 5 ul 1 x RNase Inhibitor 40 U ul 0 5 ul 20 U Recombinant DNase RNase free 2 10U DEPC 42 5 x ul 50 fup 2 37 20 3 DNase A 1 2 5 ul 0 5 M EDTA 80 2 2 DEPC 100 fl up B 1 50 ul DEPC 100 ul 25 24 1 2 15 000 rpm 5
15. PHO 0 m PNO10 IdRaRa PrimerArray PrimerArray Embryonic Stem Cells s PrimerArray Embryonic Stem Cells Human PHO16 PrimerArray Embryonic Stem Cells Mouse PNO16 vV201209Da PrimerArray Embryonic Stem Cells RT PCR ES 88 8 RT PCR PrimerArray AnalysisTool for Embryonic Stem Cells Multiplate RQ 2
16. 2 URL http www takara bio co jp primerarray_tool2 1 Thermal Cycler Dice Real Time System MRO TP960 Thermal Cycler Dice Real Time System Single MRO TP870 Multiplate RQ Thermal Cycler Dice Real Time System RNA 1 PCR 2 5 3 5 cDNA 20 RNA 30 RNA DNase 1
17. PrimerArray 20 a A AE PrimerArray A PrimerArray 4 lt I 1 PrimerArray 20 0 I PrimerArray 2 PrimerArray 4
18. Relative Quantity Chart Gene Profile Relative Quantity Chart Hglatwe Muahtity ond Deereatie Tarqetassociation Gene Profile Hglatiwe uahtity Cond erate 2 3 CIIEI H I 7 BE ssociation http www takara bio co jp 12 PH016 PNO016 IV Appendix RNA RNA RNase 0 RNase RNA RNase A RNA 1 2 1 180 60 2 0 19 DEPC
19. 3 2 ul 4 PCR 95 30 2 Step PCR 95 3 60 30 40 http wwwr takara bio coJp 14 PH016 PNO016 PrimeScript RT Master Mix Perfect Real Time RR036A SYBR Premix Ex Tag 11 Tli RNaseH Plus RR820A B PCR Thermal Cycler Dice Real Time System TP900 Thermal Cycler Dice Real Time System MRO TP960 Thermal Cycler Dice Real Time System Single TP850 Thermal Cycler Dice Real Time System Single MRO TP870 96well Hi Plate for Real Time NJ400 Sealing Film for Real Time NJ500 NucleoSpin RNAII 740955 10 50 250 RNAiso Plus 9108 9109 Recombinant DNase RNase free 2270A B PrimerArray Primer Set ID 5
20. 37 1 DEPC 120 30 RNA RNA 180 60 DEPC 0 1 DEPC RNA 2 PCR I Applied Biosystems 7300 7500 Real Time PCR System Life Technologies 1 cDNA PCR 1 110 SYBR Premix Ex Tag ll Ti RNaseH Plus 2 Xx 25 ul 2750 ul ROX Reference Dye or Dye ll 50x 1 Al 110 ul cDNA 50 ng ul 2 0 8 ul 88 wl dH2O 15 2 ul 1672 ul Total 42 ul 4620 1 Appl
21. ied Biosystems 7500 Real Time PCR System ROX Reference Dye ll 7300 RealTime PCR System ROX Reference Dye 2 total RNA 2 PCR 42 uu 3 2 PrimerArray 8 ul http wwwr takara bio coJp 13 PH016 PNO016 4 PCR 95 C 30 2 Step PCR 95 C 5 60 31or34 3 40 3 7300 31 7500 34 Applied Biosystems 7500 Fast Real Time PCR System Life Technologies 1 cDNA PCR 1 110 SYBR Premix Ex Tag ll Ti RNaseH Plus 2 Xx 10 ul 1100 ROX Reference Dyell 50 xX 0 4 wl 44 wl cDNA 50 ng ul 0 4 ul 44 ul dH2O 6 ul 660 Total 16 8 ul 1848 wl total RNA 2 PCR 16 8 ul 3 2 PrimerArray
22. ttp wwwr takara bio coJp 10 PH016 PNO016 4 PrimerArray Analysis Tool for Embryonic Stem Cells Multiplate RQ PrimerArray Analysis Tool for Embryonic Stem Cells 1 Scatter Plot Fold Difference 3D Profile Scatter Plot 3D Profile 1 Fold Difference Oo_ 1E 04 1E 03 1E 02 1E 01 1E 00 1E 01 1E 02 Control Sample Scatter Plot Tu Tu 1 Q 3D Profile Fold Change 11 PHO16 http www takara bio co jp PNO016 Multiplate RO
Download Pdf Manuals
Related Search