“WAKO BIO WINDOW” No.59(2004.6)
Contents
1. 505 0 8mA 19M0 0 000000000 a 1 2 3 4 1 2 3 4 0 iA Lane 1 Marker Precision Plus Protein Standards Lane 2 restain Kaleidoscope Prestained Standard Lane 31 k Lane 4 IDE VIEW Western Size Marker UN 06 233 02211 20000 00000000 000 No enma 160 209716 7 4 1019 48006 206 15851 5 74 x 12119 4 800 j 1 ia ERNAL 7725 540 8665 00000000000 10 20 0606203 1788 1 9 4103 0023 00000 0000 00000 50 130 0303270 82431 00009 0 00098 0921622 1005 119 0 0 0 92 045 476 206R 0 02. DABI 0000000000 o 4 ImmuChemIHC Kiti 0 0 0 0 00000 0000 00000 0000000000000 20910 0 000000000000 4001 0 00000 00000 00000 0000 o 00 o 0000000000 0 0 IsHybISH 0 I 0 0 0 00000 00 181100 100 slides 1 181500 500 slides gt Normal Serum 05m 25m gt Biotinylated Antibody 02m 10779 gt 5 05m 25m gt Biotinylated 5 25m gt 10X 10179 5 0 7 gt Liquid DAB 106 5 0 gt Detoxification 1 5 0m 1811004 l Kit BioChaing W Antigen Retrieval Dewax Solution
3. o 00 0000 191 0 0 0 9 000 0000000 586 791216 82311006 1 0006 585 79135 282315006 Antigen Retrieval Dewax Solution 45 0006 580 79141 8231000 _ 600 O Bio Window No 59 JUN 2004 BioChaing 51 0 o IsHyb In Situ ISH Kit In siu 0 590 00 00000 0 000 0 0 0 0 0 I I 0 0 00 0 0 L 5 O BioChaini 0 IsHyb ISH 00 0000 0 00 0 0 0 0 0 00 000000000000000 o 00 0000080 000 15 000000 RNase 0000000 000 0 00000 0 1590000006 009 e IsHyb ISH 530 0 0 0000000 000006 401 1001 4001 0 u K2191050 50306270 gt Prehybridization Soluti
4. o 000 0000 12 34 56 7 910111 w w w __ w 30 601 60 5 5 200 12 0 0 No 194 129610 0 1 2 11 12 134 14501 4 10 No 131 145110 5 6 7 8 9 5 101 90 5 91 2 51 o0 0 6 9 QSN 31 0 209 20 00 000 000 00 0 00 0 00 0 00 0 00 0 000 0 300 32 12 o o 34 34 iong 0 09
5. o 000 0000000 0 09 0 00000000 580 76601 814366 N 1 Pyrene Maleimide 100 12 0006 588 766416 814456 Tetramethylrhodemine 6 Maleimide SingleIsomer 5mJ 22 0006 587 76851 Acrylodan 6 2 Dimethylaminonaphthalene 5mg 12 000 0000 00 000 0000000 0 09 0 00000000 589 766716 880586 Rhodamine B Ester Perchlorate 12 0006 589 764316 84717 4649 1060 330006 587 7661 880656 Tetramethylrhodamine Methyl Ester 25 21 6006 581 764916 817126 DiO 33 Dioctadecyloxacarbocyanine Perchlorste 100 22 0006 580 767216 880616 TMRE Tetramethyirhodamine Ethyl Ester Perchlorate 25 21 6006 581 76751 84905 _ 1 163 1 1 Dihexadecyi 3 3 3 3 Tetramethylindocarbocyanine Perchlorae 100mg 22 000 589 76811 84714 _ DiOC5 3 3 3 Dipentyloxacarbocyenine Iodide 100mj _ 12000 586 76941 84707 DiOC7 3 3 3 Iodide 100 12 000 000000000009 000 0000000 0 09 0 00000000 588 765214 810046 6 6 Carboxyfluorescein 00 12 0006 586 76561 8324 Ethidium Monoszide Bromide 22 0006 583 764516 822566 Coelenterazine cp 2501 g 330006 581 766316 81528
6. 4 4 4 lt lt gt lt gt lt gt lt gt lt gt 4 gt lt gt lt gt lt gt lt gt lt gt gt gt 4 gt gt lt gt T Receptor Signaling Pathway Westem Blot with Ant ZAP 70 clone 2F3 2 Jurkat cell lysate was probed with Anti ZAP 70 clone 2F3 2 0 5 g ml 000 0000000 0 0 0 0 00000000 569 172316 05 2530 Anti ZAP 70 human clone 2F3 2 Monolconal 250196 62 0006 16 2106 Anti 2 70 human FITC 10019606 780006 563 16891 06 2716 Anti ZAP 70 1504 560006 562 55681 14 404 2 70 active 2019 64 000 U K Bio Window No 59 JUN 2004 3 00000100000000 IYRIZ I ECOS Competent E coli DH 000000 o 5960 Hi Competence Broth 0 319 01343 0 000 1 191 250 0 o soc 1 o 009
7. 000000000 H RATAN AA coo 2 000 No 1 59 0 4 165 21364 average M W 200 000 500 000 525006 Pa 4 2 162 2137464 1 500 000 2 500 000 57 0006 z136 Glutamic 46 a 169 21384 average M W 4 000 000 6 000 000 500 61 500 0 000 0 00 D 00000000009 209 30006 1000 15 0006 Poly y Glutamic Acid Sodium Salt average M W 200 000 500 000 u 22521905 Poly y Glutamic Acid Sodium Salt M W 1 500 000 2 500000 49 500 Polyy Glutamic Acid Sodium Salt gt 168 214139 M W 4000 000 6 000 000 1 166 21414 54 000 10 Bio Window No 59 JUN 2004 0 ELISA 000 c 0000 0009 009 lu 50009 9 0 0000000 0 Bio Window No 59 JUN 2004 11
8. 0 00 0 7 0 0000000000000 0000000009 00 00000 7 50000000000 0 0 0 0 0000 0 0 0 0 0 0 0 1760 00000 00 o IPTG Induction E coli RNA 7 7 1 _ Target gene TZ RNA polymerase s 1 mF I lac promoter T7 promoter lac repressor l lac gene HOST CELL E coli genome 0 00 0 04 0009 0 0000000000000000000000000000000000000000000 o 000000000 000 0 0000000000 o 000000000 Wako Bio Wincow E mail biowin wako chem co jp 06 6201 5964 Bio Window No 59 JUN 2004 000 000 0000000 0 09 0 00000000 586 77921 60015 36 328 0 10000 99 300006 584 77961 69661 36 1580 000 10u g 27 0006
9. O WakoPURE system WekoPURE systemi 303 IF1 IF2 IF3 0000 EF Tu 5 0 0 0 0 1 RF2 201 0 00 0 000 7 0 00 0 0 0 0 0 00 00 00 0 00 0 000 00 o o 0000000 o RN e 000000000000 o 0 __ WakoPURE system 00002 00000 DNAD O 0 0000 00000 000000000 ej 0006060 DHF Ri Dihydrofolate reductase 2 3 4 1 0 000000 2
10. E Hind 000000001108 00000000000 000 PVDA 0 PVDF OD mamaman 00000 2 000000 g 7 _ 00 0 0 PVDF 0 IDD DU D 000 809 100 00000000 0 00 2 0 00000 004800 mtam A DNAD 000 0 065816 0000000000000 0000000000000000 0000000 000000 0 0 000000000006 000 0000000 0 09 0 00000000 367 00521 _ 66543 00000 30cmx 0 109 21006 36405411 66542 0000000 PVDF 20 20 0 10 16200 000000000000000000 Wako Bio Window No 59 JUN 2004 1 00000000 00 1004 00000000000000 260 6 21 0000000 794 0 0 20 0 1000000 33 00000000000 z Esas m0 00000000 0 000 00000000 e000000000000000
11. 5 000000000000 0 000 0000 00000 0 0 209 0 0 0 0 8 000000000 1 o 5 520 12well 0 0000000 20 00000000 9 12 34 56 7 89101112 1234567 8 9101112 s 123456789101112 12 34567 8 91011 000000 5 5 200 No 194 129610 0000000000000420 000000000 00000 20 00000 20 4 00000 800 00000 1200 000000 178 00551 4 000000 00 178 00551 00000 u 0 8mA cm 10 00000 0 8 10 0 PVDFI 0000 000000 Lane 10 Kaleidoscope Standard 5u 7 0 200000000 5 20000 134 145011 Lane 20 Precision Plus Standards 5u 0 8 56 200000 131 1451111 Lane 30 Western 5 Lane 9 Lane 4 WIDE VIEW Western 5u Lane 100 WIDE VIEW Western 1u Lane 5 Mid Range Prestained Marker 5u Lane 11 WIDE VIEW Western 5u Lane 6 10kDa Prestained 5 0 Lane 120 WIDE VIEW Western Marker 101 0 00 0 n ooon 0000000 genomo 192 12901
12. Lane 3 000 Lane 4 00 0 0000 3000 12500000 0 0 0 000000000000000000000 WakoPURE system 09 05 0000000000 0 WakoPURE 0 00000000 000000000000 000000000000 10 200000000000009 2 Window No 59 JUN 2004 000000 0400 00000 01100 gt 0 e 000 e 0000006 Shimizu Y Inoue A Tomari Y Suzuki Yokogawa T Nishikawa Ueda T Nature Biotechnology 19 751 2001 0 00 No 0 09 09000000009 299 59501 410 160006 295 595 system 16010 49800 0 00000000000000000000000000000000000000000 1K e cell signaling solutions 70 000000000 0 0 00 0 0 2 701 2 701 SLP 76 H 0000000000000 o gt lt gt 4 gt lt gt lt gt lt gt 4 lt gt
13. 7 5 2well p 40000 20000060 10 12006 199 1291196 SuperSep 7 5 1001 2 000 10 12 0006 196 12921 10 2well 20000 130009 120000 193 2931 SuperSep 10 17well 501 500 10 12 0006 190 12941 SuperSep 12 9 12 1 DA 14000 80 00 120006 197 12951 SuperSep 12 5 17well 301 300 10 12 0006 194 13061 SuperSep 19 126 9 6000 600060 180006 191 130719 5 15 17well N 201 30 119 180006 194 12961 SuperSep 5 20 12well BA 10 000 200 000 120006 191 129719 SuperSep 5 20 17well 501 750 10 4 12 0006 198 12981 SuperSep 10 20 12well RA 10 000 130 0006 00 120006 195 12991 SuperSep 10 20 17well 501 500 19 12 000 Q K TA Wako Bio Window No 59 JUN 2004 21 o 2 D Sample Preparation for Nulear Protein 00000000000 2 000000000 00 Proein 0000000000000000 00000000000000000 2 0 0000000000000 0 0000 0250 0 0 00000 gt Nuclear Cytoplasmic Extraction Reagent Nuclear Extraction Cytoplasmic Extraction ReagentI Cytoplasmic Extraction Reagent 10 gt 2 D Sample Buffer for Nuclear Protein 2 D Sample Buffer Component A 2 D Sample Bu
14. 229 0000000 26 0 CHO _ 0000000 9 MG 63 00 COS7 Veo MOLT 4 T 00000 3 0000000000 K52 _0000000000 SFME 987 00000000 ESD3 00000000 293 000 u STO 00000 0 000000000 000 UD e __ 696 0 ____ 3429064 DFOL _____ 1 2500 0 0000 0 570 0000 12 Bio Window No 59 JUN 2004 0000000 0 00000 0 00000 000 0000 00 0 0 000 00000 00000 o 0 000000000000000 o 0000000000 000 0 o 000 0000000 0 u 0 00000000 589 765514 811246 5 6 TAMRA SE 5 and6 Carboxytetramethylrhodamine 25 22 9006 584 76621 811256 5 SE 5 Carboxytetramethyirhodamine Succinimidyl Ester 5mg _ 22 9006 581 7661 811266 6 SE 6 Carboxytetramethyirhodamine Succinimidyl _ 5 22 9006 584 764814 811156 6 SE 6 Carboxy X Rhodamine Succinimidyl Ester 5mJ 22 9006 585 76911 81008 6 5 290006 588 76881 8115 5 CR6G SE 5mg 22000 00000000009
15. 0 09000000109 174 00553 __ 250 42006 a Mae n K TA Wako Bio Window No 59 JUN 2004 19 O SuperSeparator O 0 000 0 0 0 0 000 210000 000000000000 00 o o 0000 00000 0 0 20000000 0 V 00 0000000 0 00000 0 0 0000000000000 6 1000 0 00000 000 00 0000000000000 09 00009 000 UDUN 0 190 13421 1019 38000 K TA 2 Bio Window No 59 JUN 2004 00000000000000000
16. Asp AMC _ 000000 16 000 4000 2 000 0 0000 000 0 000 l 00000 0 340m0 0 0 00 429 27000 0 j 0000 000 000 00000009 7 00009 00000000 592 32881 MNA120 2 00000 0 13 400 14 Wako Bio Window No 59 JUN 2004 0000000 Q Val Asp OPHI in vivo 000 0000 0000 00 0000 0000 o o 0 000 000000 mg 0000 000 00 000 00 00000 0000 o 000 0000009 ___ 09 0000009 0 00000001 599 32293 OPH109 Q Va AspOPH 0000000 Img 143000 597 329051 AL010 Ac Asp Glu Va Asp Aldehyde 00 0 0 00010 lm 7 5006 594 329614 AL012 Ac Ile Glu Thr Asp Aldehyde _ 0000009 1mg 7 500 591 32971 AL022 Ac LeurGlu His Asp Aldehyde 0000 lm 7 5006 598 32981 AL026 Ac Leu Glu Va Asp Aldehyde 1000009 lm 7 5006 505 32991 AL013 Ac Tyr Val Ala Asp Aldehyde 0000 Img 7 5006 592 33001 0096 Ala Asp Aldehyde 0000000 lm 7 5006 599 33011 015 Ac ValGlu Ile Asp Aldehyde 0000009 1mg 7 500 00 00000 o 000 00000009 ___ 09 0000000 509 32891 DK010 2 ___ 0000000000000 12600 592 31823 DK006
17. 0000009 Img 126006 592 32901 DK001 LerDMK 10000000000000 126006 599 329114 DK007 _Z Leu Leu Tyr DMK 12 6006 596 32921 DKOB 2 0000009 12 6006 593 32931 DK002 2 1000009 126006 590 32941 DK009 _ Z Tyr Ala DMK 00000000 lm 12 6006 595 317936 CK032 2 _ 00000009 Img 17 6006 598 327416 CK022 ____ 00000000000001 9 134006 595 3251 OK22C Biotin Ala Ala Phe CMK ___ 0000000 17 6006 592 32761 17 0 0 0 000000 134006 59831783 CK023 __ 0000000 Img 134006 509 32771 CK036 Ala 000000 Img 134006 56 32781 CK021 AlaAlaProVa CMK ____ 000000000 __ 13 00 593 32791 CK020 _ MeOSuc Ala Ala ProVa CMK 000 0000000 106 134006 5063280 CK026 1000 1 Img 134006 59431763 CK008 Z Gly LerPheCMK ___ 1000000 134006 598 328114 CK001 L Le CMK 0000000 0000009 Img 134006 590 32821 MeOSuc Leu Ala Pro Phe CMK J 000000 134006 597 32831 CK034 CMK __ 1000009 134006 504 328416 CK009 2 000008000 Img 13400 B 0 mg 591 31773 CK01
18. CONTENTS 0009 WakoPURE system I A p 2 ECOS Competent E coli DHA p 4 Novageni p 6 BioChaing IsHyb In Situ Hybridization ISH p 9 DoFect GT1 12 BioChaing ImmuChem Immunohistochemistry IHC QQ p 8 Antigen Retrieval Dewax p 8 ulus p 11 2 70 p 3 0009 0 0 000 E 9 10 0 000 p 13 00000 0000000000 0006 14 00000 0000000 0000000000000 16 SuperSeparator Epi 20 0 Quick CBB PLUS 19 Supersep 00000 0000 0000 0 00 21 2 D Sample Preparation for 22 2 D Protein Molecular Weight Marker p 23 WIDE VIEW Western Size Marker p 24 000 000 0 0000 2004 iw 18 23 050000000000 23
19. 00000 57 Competent E coli DHA 9 42 591 301 20000 4 Bio Window No 59 JUN 2004 0 EcoS vu6 0 00000 09 oe 42 1 5 0 o 00000 Ecos 1 04 ECOS Competent DHA 0 00000 0000000 DH 0 0 0 0 pUC19 DNAD 000000009 000 Ecos 0000000000000 0000005 00000000000000 00000000 37 00000000000 o 00 009 0000000 0000000000 8 DH 0 0 0 DH 0000000000000 2 mnnn kn 0 17 1 00 12 z E Ligation Convenience 0 0 09 Liga
20. 581 77971 6966236 _pET 1660 000 10u 27 0006 588 77981 69739 36 208 0 1000 10u 27 0006 585 77991 69741 3 218 01000 g 27 0006 574 767016 69820 36 7 1 000 2ug 260006 582 78001 60864 36 288 0 000 27 0006 589 78011 69865 36 288 0 2000 27 0006 580 78041 70561 36 428 0 11000 300006 584 78061 70939 36 43 18 0 10000 99 300006 588 78081 0000 0000 10u g 462006 585 7811164 11 000 9 20u 99 47600 582 78121 pEx 1000 20u 99 47600 583 78031 70548 36 T710 00 10 31 000 g 358006 583 77931 69337 3 7100000000000 500 138006 580 77941 69348 3 T70 000000000 500pmo gt 13 8006 586 78021 69896 3 000 0000 20u g 9000 571 761 60449 46 81210 00 00000 Im 27 0006 577 76671 60450 36 8121 DEJN 00 00000 1460 573 76673 69450 4 8124 DEJN00 00000 Im 27 0009 574 76681 69451 36 8124 DE3 pLysS UU 0000 0 O4m 14000 570 76683 60451 46 8121 00 0000 0 lm 27 0006 587 77951 69454 46 5174 55 00 0000 0 Im 27 0006 572 76981 69825 46 NovaBlue 0000000 Im 27 0009 578 76721 609946 3 0 0000 02 10kbp 2510659 195006 57076021 6500230 DNA 0 0000 03 109 100 19500 577 769831 69149 3 00000 0 00000 15 150kDA 100lanes 19 5006 574 769414 69
21. 0 III T I pvp E u uuu ss 96 0 0 8 12 1 F gt u E u 200 1 9 REEE 2 EE NISPAS E A EE 60m 1 u EAER 100p 71 25 1001 1 uu E 12 1 y D l U u u u u u 1211 11 u uu A 1001 1 000000 000000 0 0 9620 00 401 000 101 1 0000 508 j 00010000 0030 0000000 000000 50 r 0001000 3 ILEI I LEEI ETIT C peptide STO pg m 00030100 000000 0009 00009 0 003 2 Mouse C peptide 100 JUD u Insulin 000000 tide 1 09 9 Insulin 000000 UIN 179504 Pro Insulin 85 0000 0000 40 000 0 Insulin 000000 20 2 Pro Insulin 000 0000009 0 00000000 638 05301 011 009 c 0000 000 75 000 000000 0 00 00000009 0 0 090000000009 639 01291 AKRIN 011 00 gt 00000 000 910 480006 63 01481 AKRIN 011T 00 000 000 T 910 480006 63
22. 0 0 00 0 D0E00000000000000 0 000000000000000000003 0 0 000 0000009 _______ 0 581 79311 89864 2 0 Sample Preparation Kit for Membrane Protein 75700 0 588 79821 89865 2 0 Sample Preparation Kit for Soluble Protein 45 800 000000000 585 79331 89866 2 0 Sample Preparation Kit for Insoluble Protein 1 45 800 2 Bio Window No 59 JUN 2004 2 0 Protein Molecular Weight Marker 0000000000 2 60000000 o 00 00 491 871 5 e 000000 2 D Marker Protein M W pl Value human plasma 6 24 Glutamic Dehydrogenase bovine liver 6 5 67 696 Actin bovine muscle 43K 526 Carbonic Anhydrase bovine erythrocytes 29 636 Myokinase chicken muscle 225 870 Trypsin Inhibitor soybeen 20KO 456 Myoglobin equine skeletal muscle IK 7074 00000 00000000 m H D F Human Apo Transferrin B Bovine Glutamic Dehydrogenase C B
23. 603206 0 00000 4 30 3 1 9 2700 363 03801 60346 00000 20 20 00 10 1600 363 00621 601066 000000000 4 3ni 0 0 19 2700 369 00601 _ 60400 000000000 4 20 20 00 10 16000 000000000000000000 000000000 Fluorotranse 00000000000 FluoroTrans 119 0 0 ne 000 0000000 0 09 0 00000000 5 0158 0 0 00000 W 7x 00 10 9 9 000 9 5 0157 00000000W 10 00 101 9 12000 BsP0159 00000000W 20 lt 20 00 10 9 160006 365 00681 22 0 0 00000 2 3 311100 17 2400 000006 000 0000000 0 09 0 00000000 020 160 0 0 0 00000 PVDF 7 84cmi 00 10 9 900 19 PM00C1050 0 0 00000 10 15 00 9 10 12000 _361 076216PvMoc2200 0 0 00000 PVDF 20 20 0 10 1600 364 0100114 020 0990 0 0000 0 PVDF 26cmx 3 3m 0 0 1 9 24006 020 1956 0 0 0 00000 PVDF 85 9 00 0 120006 00 16 0 00000 0 PVDF 13 00 1200 000000000 00000000000000000 9
24. His Asp AFC 000000 12 600 596 31583 1206 Ala 0000004 12 600 503 316136 AFC142 _Ac Val Asp Va Ala Asp AFC 00 10 700 597 325714 70 Glu His Asp AFC 2000000 10 700 594 3258149 AFC171 Ac Val Asn Leu Asp AFC 000000 12 6006 501 325916 AFC141 Ac Vad Glu lle Asp AFC 0000009 12 600 504 326016 2014 000000 12 600 591 32611 Ac Val Lys Met Asp AFC 0000000 12 600 0 00 0 00 0 0000 0 0 0 0 0 0000006 380 0 0 00 460nm 000 No D D UD U UIDUD 508 32621 AMC153 Ac Ile Glu Pro Asp AMC 0000000 12 600 505 32631 AMC145 Ac Ala Glu Va Asp AMC 0000000 e 12 600 599 31713 AMC138 Ac Asp Glu Va Asp AMC 00000000M 12 600 5902 32641 AMC144 00 12 6006 599 326516 140 Ac Ile Glu Thr Asp AMC 000000 12 6006 506 326616 1546 Ac Leu Glu His Asp AMC 0100000 e 10 700 503 326716 1574 Ac Leu Glu Thr Asp AMC 0100000 e 12 600 590 326816 AMC155 Ac Leu Glu Val Asp AMC 0000004 12 600 597 326914 AMC156 Ac Trp Glu His Asp AMC 0000004 12 600 590 32701 1206 Ac Tyr Val Ala Asp AMC 0000000 10 7006 597 327114 1414 lle 0000000 12 6006 594 32721 1796 Ac Val Glu Thr Asp AMC 0000000 12 600 591 32731
25. 0 0 098 0520772 0788 0 09 0 0 0 0928 0290858 2278 0 9 0 00098 0221222 3072 119 0 0000 928 011 71 0285 0 9 0 0 0 98 082 285 6381 0 0000010 0 0 0120 052 099 100 0000 0O 0120 052 806 O biowin http www wako chem co jp 04 215 61 01 DN 6 01
26. 000 0000 O PEST 00000 00000000 5 0 000 o _ 0000000 7 x 000000000000000 o TE 8858 1 444444 ha d O 1 00000000000 0 URL http wwwsochemcojp 0 000000000000000 0000 000 0000 0009 000000 0009 0000000000 000000 0009 00000000030102 2 0000000000040 50 33 TEL 06 6203 17886 TEL 03 3270 82436 FAX 066201 5964 _ FAX 0332708582 _ O g g 0 o A 18 Bio Window No 59 JUN 2004 O PLUS 00 00
27. 0000000000000000000 000000000 Plusg 00000 19 0000000000000066000000 000000000000006600000 0000000000000000000000 9 0000000000000000000 00000000 e I 0 6 0 7 0 8 0 9 0 10 0 11 0 pH NHs Z 4294752 Z 8 H H 8 w m Emi A 84 Hind 0 O 00000 0 1000 1ng 0 000 0 0 00 0000000000 000000000000000 o 00000 0 0 T Molecular Dynamics 16 Bio Window No 59 JUN 2004 0 00000 00000000 000 0000000 0 09 0 00000000 361 00541 60106 4 30 3 1 9 2700 361 03461 601006 4 20 20 0 00 10 160006 369 00581 60207 000 3n 00 1 27 00 362 03631 602006 0 00000 B 20 20 0 00 10 160006 360 05751
28. 000000009 1 10 cfu u g pUC19 DNA ECOM elin unn 09 00000000000 q ECOS 110 00100 4 i 0 9 00209 000000 19 0000000000030 Y O 0 00 50 000000 UD Ecosmw6 00000 U 0009 4261 450 00000 00 000000000030 000000 6 gt 0 0000000000 0378 0 0 0 00040 0 Ecos 10 0 000000000 III 429 3 000000000 9 4 00000 00 0 0 0 0 0 000 0000000000 0020000 0000000 500 gmg 5 0000000 25 00 50 000000000 12 51 009
29. 278 3 000 591 2000bp 50lanes 19 5006 50231461 70967 36 0000000000 0 0 Im 41 000 508 31463 70967 46 0000000000 0 0 280 000 575 76971 69755 36 00000000 lkit 195006 587 780514 49 HisTad 00000 7 0006 578 76961 69670 3 0000 lm 168006 574 76963 69670 4 00009 66 000 570 76661 69066 3 000 0000 19 500 581 78071 71110 36 0000 0 0000 10u 58006 571 76601 69522 3 7 00000000 50u 28000 507 31411 70921 4 0 0 0000 10xX 41 8006 575 76731 70584 3 0000 00000 000 100m 11 000 577 76735 7058449 000000 00000000 500m 42 800 570 3272141 70746 3 000 0000000 901 10 0000 180006 573 327114 7066439 000 90000000 97 10000UNQ 23 5006 585 78091 71300 3 00 000 00000000 00 00000 1 9 98006 588 78101 71366 3 000000000000000000002 00 10 17 500 Window No 59 JUN 2004 Biochaing 000000000009 ImmuChem Immunohistochemistri 0
30. 3 03411 0819 0 00000 00m0 100 997 0 62 0006 295 57401 0 00 ELISA 96 75000 G T DOJNDO DoFect GT1 d o 00 000000 6 0 0000000 o 000000002000000000000 o 9 vortex 0 oe 0 0 000000 000000 GFP U 0000 0 000000600 00000000 0000000000 2 000000 00000 0000000000 0000000000 0 00000 0 0 00 00 0 0 0 0 0 2 00000 0 0 00 000 00 000 9 0000000000000000 00000000 00000000000 gt Cell Line Cell _ 00000 A126 _ 0000000 UtSMC 000000000 PA 1 10000000000000 A549 MCF7 0009 MRC 5 000000 Hc HepG2
31. 3 D Phe Pro Arg CMK ___ Uuuuun 134006 508 32861 CK035 _ MeOSuc Trp Ala Pro Leu CMK 00000 000000000000 9 Img 134006 5095 32871 D Vali Leu Lys CMK 0000000 13 400 0 0 00 000 000 0000 00000 0000000 0 0000 Ti milli 000000000 Wako Wincow E mail biowin weko chem co jp 06 6201 5964 Window No 59 JUN 2004 15 00 00000 0000000000000 19831 00000 000000 m0 0000 0 00 661 0 9 1000000 0 00000000 6 Hind 4 14 4400000000000000 2 000
32. 6 ANTS 8 Aminonaphthalene 1 3 6 Trisulfonic Acid Disodium 100mg 12 0006 58476741 8461060 5 199 20906 580 76581 TMA DPH 235mg 22 000 Wako Bio Window U TN No 59 JUN 2004 13 Biomedicals Inc TW O 00000 0 00 ICN Biomedicals ESQ 000000000000 0000000000 3760 00000000 0 00 0 000 0 00 00 0 00000 0 0 0 0 00000 04 O 400m 0 0 00 S05nm 0 0 000000 o 0000008 ___ 09 C 0000 590 31623 1536 Ac Ile Glu Pro Asp AFC 0000000 12 600 595 325116 145 Ac Ala Glu Val Asp AFC 0000000 e 12 600 593 315903 AFC138 Ac Asp Glu Va Asp AFC 000 0 00 0 0 12 6006 592 325216 2026 Asp AFC 0000 12 600 599 32531 AFC144 __Ac Asp Met Gin Asp AFC 0000000 12 6006 596 32541 1696 Ser 000000 12 600 506 31603 1406 Ac Ile Glu Thr Asp AFC 000000 12 600 503 325516 158 Ac Leu Glu Glu Asp AFC 00000 B 12 6006 590 325616 1546 Ac Leu Glu His Asp AFC 0000004 13 400 591 316536 1574 Glu Thr Asp AFC 000000 12 600 597 31633 1556 Ac Leu Glu Val Asp AFC 000000 12 600 594 31643 AFC156 Ac Trp Glu
33. ffer Component B gt Protein Desalting Spin Column 00 2 0000000 98 0 0000 000000000 92 2000000000000 000 000000000 2000 0000 1 0000000000600 00000 a 2 002 00 mgmy NE PER Nuclear and Cytoplasmic Extraction Reagent 90 00000 80 16 5 5 pH4 151 e D00008 000000000000000000000 00 22 000000000 000000000009 0000 5000 gt 0000000000000 B B _ 00009 lt gt ell 4500x 41 10 0 0 0461 1500 g 10 0 0 el T 1500 9121 9 1500 91210 0 9 00002000009 Collection Tube 0 0 0 ollection Tube 0 0 9 Collection Tube 00000000000 0 0 o 00 0009 000 000000 9 _______ o 0 0000000009 584 79801 89863 2 0 Sample Preparation Kit for Nuclear Protein 1 6880 0000000 0 Cytoplasmic Extraction Reagenti IO 00000 0000 00000 00 0
34. on 25Mm gt Hybridization Solution 25Mm _____ ___ 12507 gt Oo Y Om 12507 20X SSC Buffer 25 0 gt 5 25 0 gt Anti Dig Antibody 251 000 0000000 0 09 0 00000000 IsHyb n Siru Hybridizatio ISHT Kit awo I T Wako Bio Window No 59 JUN 2004 9 o O 00 0 00000 0 000000000000000000000000 000000000000 o Bacillus subtilis 20 0 6001 905 o 21 6 000000 00 DLO 00000m bD 73 o
35. ovine Muscle Actin D Bovine Carbonic Anhydrase Chiken Myokinase Soybean Trypsin Inhibitor G Equine Myoglobin 000 0000009 _______ 0 _____ zo PanMan lt 09 09 000000 6 90 11 0000000000000 093000000000 o 8 000 00000 00000 1 0D o O 10 160 60 90 12 450 13 45 000 000000000000 0000 o 92 000000000 00009 2200 00009 Wako Bio Window No 59 JUN 2004 23 O WIDE VIEWTM Western Size 00000000000000 o 160 150 0 oe e 9 04 11 5 1 lane 0000 16 150 kDa 000000000000 0 S
36. tion Convenience Kit COSTM Competent E coli 000000000000000 000000 00000000004 00000000000 o Ligation Convenience Kit ECOS Coreetent E coli DH 4 DN 20070 22888 16511 a m Ligation Convenience Kit DNA DNAD 00000 10 6 2 2 Ligation Mix ECOS E coli 0 09 00000000 000 u u 000000006 315 05963 _ 10 32006 319059616 Ligation Convenience 1011 20 0006 319 01343 Hi Competence Broth 1m x 20 4 18 000 Bio Window No 59 JUN 2004 2 14 pETI 0 T7 000000 Space Cytoplasm Inner Membrane Cell Wall Outer Membrane 7 590 0 2 7
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