取扱説明書 - 東洋紡ライフサイエンス事業部
Contents
1. 2 PCR 3 Tm 55 C lt 65 C 60 15 5 Channel F1 2 data collection PCR2. 60 15 5 Detector SYBR Quencher None data collection 30 2 2 data collection PCRH 1 1 3 60 72 95C 60 95C 15 x40 60C 15 72C 45 data collection PCR 2 2 60 95 C 60 95C 15 x40 60C 60 data collection 2 Roche LightCycler 1
3. 4 QPK 201 1 20C 4C 3 1 PCR
4. 2 PCR 6 ReverTra Ace cDNA ReverTra Ace w 100 FSK 101 PCR cDNA ReverTra Ace qPCR RT Kit 200 FSQ 101 PCR cDNA ReverTra Ace qPCR RT Master Mix 200 FSQ 201 DNA PCR cDNA ReverTra Ace qPCR RT Master Mix 200 FSQ 301 with gDNA Remover 10 000U x 1 TRT 101 ReverTra Ace DNA RNase
5. 20 30mer GC 40 609 200bp 150bp 50 lt 150bp PCR PCR ABI PRISMP7700 LightCycler LineGene PCR 2 DNA cDNA 1st strand cDNA cDNA dT
6. PCR cDNA ReverTra Ace qPCR RT Kit Code FSQ 101 209 1st strand cDNA ReverTra Ace w Code FSK 101 10 cDNA PCR DNA PCR DNA DNA 110ng DNA 4 4 1 ABI PRISM 7700 1 PCR 0 4M 50w
7. 55 10 20 5 30 5 5 200bp 15 data collectton 10 Detector F1 Temperature Transition Rate 20 sec
8. LightCycler PCH 2 QPK 201 QPK 201X5 50L 50L x 200 x 1000 SYBR Green a Realtime PCR 1mL x5 1mL x5 x5 Master Mix 2 SYBRY Green Realtime PCR Master Mix dNTP MgClsz Taq DNA Taqg PCR SYBR Green gt DNA 1 20 10
9. LightCycler 20L LineGene 10 L 0 2 0 6 M 3 PCR PCR 1 2 3 42C 20 5 PCR 1 2 3 Tm
10. 2 8 TEL 06 6348 3786 FAX 06 6348 3833 E mail order_lifescience toyobo jp 141 8633 10 2 TEL 03 6422 4819 FAX 03 6422 4951 E mail order_lifescience toyobo jp TEL 06 6348 3888 FAX 06 6348 3833 9 00 lt 12 00 13 00 17 00 E mail tech_osaka toyobo jp URL http www toyobo co jp bio
11. PCR GO cDNA cDNA PCR
12. 2 data collection PCR 3 55 72 95C 30 95C 5 x40 55C 10 72 C 15 data collection Melting Curve 3 BioFlux LineGene 1 PCR 0 4M 10wL 2 2 AL SYBR Green Realtime PCR Master Mix 5 AuL 1 10 M 0 4 wL 2 10wM 0 4 wL 2 AL 10 gL SYBRY Green Realtime PCR Master Mix 1 02 0 2 0 6ux M
13. ReverTra Ace ReverTra Ace RNase Inhibitor 5U L ReverTra Ace RT RT RT DNA DNA RT RT ReverTra Ace 3U L 0 3U L RT RNase Inhibitor 5U L 32 AL ReverTra Ace 100U L 1 AL 33 AL PCR 0 4M 50wL 11 AL SYBR Green Realtime PCR Master Mix 25 L 1 10 M 2 AL 2 10M 2 AL RT ReverTra Ace 3U L 5 AL 5 AL 50 L SYBRY Green Realtime PCR Master Mix 1 2
14. PCR PCR GC 3 1 PCHR
15. 55 lt 65 C ABI PRISM 7700 42 C 20 95 C 5 95 C 15 x 40 60C 15 72C 45 data collection Roche LightCycler 42 C 20 95 C 5 95 C 5 x40 55 C 10 72C 15 data collection BioFlux LineGene 42 C 20 95C 5 95C 15 x40 60C 15 72C 30 data collection 5 1 1 SYBRY Green 2 PCR
16. 3 60 72 95 C 60 95C 15 x40 60C 15 72C 30 data collection Melting Curve 4 1 RT PCR 1 cDNA DNA DNA PCR RNA 1 RT PCR 1 RT PCR PCR ReverTra Ace Code TRT 101 2 PCR RNase Inhibitor Code SIN 201 0 5U L ReverTra Ace 0 01 0 3U u L
17. RNase Inhibitor recombinant PCR Realtime PCR Master Mix PCR 10 000U x 1 x 5 10 000U x 1 x 10 2 500U x 1 2 500Ux 1 x5 1mL x 5 1mLx 5 x 5 TRT 101X5 TRT 101X10 SIN 201 SIN 201X5 QPK 101 QPK 101X5 SYBR Green NIRS 20 SYBR Green Realtime PCR Master Mix Plus 1mLx5 x5 1mLx1 QPS 101T PCH 1 67mL x 3 QPS 101 THUNDERBIRD Probe qPCR Mix 1 67mL x 3 x5 QPS 101X5 SYBR Green PCR 1 0 0 0 THUNDERBIRD SYBR qPCR Mix 1 67mLx 3 x5 QPS 201X5 OR POR 500g Lx5 QRT 101 Lx5 x5 QRT 101X5 RNA direct Realtime PCR Master Mix S00LXS5 XS5 1 step PCR SYBR Green 500NL x 5 QRT 201 RNA direct SYBR Green 500Lx5 x5 QRT 201X5 Realtime PCR Master Mix 530 8230
18. DNA 2x SYBR Green I Molecular Probes Inc PCR 2 LightCycler BSA ABI PRISMP7700 Taq PCR 10 1
19. L 16 L SYBR Green Realtime PCR Master Mix 25 uL 1 10w M 2 AL 2 10M 2 AL 5 AL 50 ML SYBR Green Realtime PCR Master Mix 1 2 0 2 0 6 M 2 PCR 3 1 Tm 55 C lt 65C
20. PCR 0 4M 20wL 6 4 L SYBR Green Realtime PCR Master Mix 10 gL 1 10guM 0 8 wL 2 10wM 0 8 wL 2 AuL 20 AL SYBRY Green Realtime PCR Master Mix 1 2 0 2 0 6ux M 2 PCR Tm 55 lt 65C
21. ldeas amp Chemistry SYBR Green Realtime PCR Master Mix Code No QPK 201 QPK 201X5 TOYOBO CO LTD Life Science Department OSAKA JAPAN A3314K 1 2 2 GED 3 3 4 4 5 5 1 ABI PRISM 7700 5 2 Roche LightCycler 7 3 BioFlux LineGene 9 4 1 RT PCR 10 5 12 6 14 LightCycler Idaho Technology Inc Roche Molecular Systems Inc SYBR Molecular Probes Inc ABI PRISM Applera Corporation 1 1 1
Download Pdf Manuals
Related Search