バイオセパレーションカタログダウンロード
Contents
1. r K Prep LAB300G K Prep FC750G K Prep FC1500G K Prep EX03KG K Prep EX06KG K Prep EX12KG K Prep EX25KGY mL min MPa mm WxDxH 1 100 15 800X600X650 1 300 1 750 1 1500 30 3000 60 6000 125 12500 250 25000 1100 X 1100 x x 700 Xx 750 X 1250 x 1300 Bioseparation products catalog 92. lgG Fc Mouse lgG Fc fragment Prepared from normal serum Pl YMC Pack Diol 200 5 um 300 X 8 0 mml D mAU 2 1 Rabbit lgG 2 Mouse IgG Fc fragment T T T T T T T T T T T T T T T T T T T T T T 1 T T T T T T T T T 0 6 10 15 20 min Eluent 0 1 M KH2PO04 K2HP 04 pH 6 9 containing 0 2 M NaCl Flow rate 1 0 mL min Temperature ambient 27 C Detecton UV at 220 nm Injection 5 uL 0 5 mdg mL uu YMC Pack C4 30 nm 5 um 150 X 4 6 mml D mAU 30 Mouse IgG Fc fragment R080619B T T T T T T T T T T T T T T T T T T T T T T T T T T T T T T T 0 5 10 15 20 25 30 35 40 min Eluent A water TFA 100 0 1 B acetonitrile TFA 100 0 1 25 45 B 0 40 min Flow rate 1 0 mL min Temperature 37 C Detection UV at 220 nm Injection 5 uL 1 0 md mL lgG lgG
3. YMC BioPro QA B Dorous Q tyDe 5 um 50 X 4 6 mmlLD 10 um 50 X 5 0 mml D 1 1 0valbumin 2 Trypsin Inhibitor mAU 2 mAU 1 2 200 200 500 ug 500 ug N080206B N080206E 100 250 ug 100 250 ug N080207C N080208C 100 ug 100 ug N080207B N080208B 0 50 ug 0 50 ug N080206A N080206D 0 10 20 30 min 0 10 20 30 min Trypsin inhibitor 5x107 e YMO BioPro QA Eluent A 20 mM Tris HCI pH 8 1 B 20 mM Tris HCI pH 8 1 containing 0 5 M NaCl 4x107 Dorous Q type 10 80 B 0 30 min Temperature 25 C 3x107 Detecton UVat280 nm Injection 100 pL xo7 1x107 Flow rate 0 5 mL min 180 cm hr for 4 6 mml D 150 cm hr for 5 0 mml D YMC BioPro QA 0 0
4. Y7M 600 8106 284 YMC 4F TEL 075 342 4503 FAX 075 342 4530 108 0014 5 29 20 601 TEL 03 5439 9790 FAX 03 5439 9791 URL http www ymc CO jD 15082K
5. YMC Triart Prep C8 S 10 um 20 nm C8 10 um 20 nm 1 YMC Triart Prep C8 S C8 10 um 20 nm 10 um 20 nm Pressure MPa m 1 2 3 4 5 6 7 8 9 10 11 Packing number Triart Prep Column size 100 X 50 mml D Packing pressure 6 5 MPa Eluent metnanol water 85 15 Flow rate 50 mL min Bioseparation products catalog 24 a MW 500 3 500 MW 1 007 MW 574 MW 556 MW 1 673 1 0xytocin 2 Met Enkephalin YMC Triart C18 4 5 3 Leu Enkephalin 4 Neurotensin 5 um 12 nm 5 YEndorphin 6 Endorphin MW 1 859 MW 3 465 TA12505 01 Column 150 X 4 6 mml D 2 3 Eluent A waterTFA 10070 1 1 B acetonitrile TFA 100 0 1 20 40 B 0 20 min YMC Triart Prep C18 S 4 5 6 Flo
6. 1Q OQ FDA PI100 500 PPI100 850 PI140 500 PI140 850 PI200 500 PI200 850 100 mm 100 mm 140 mm 140 mm 200 mm 200 mm 50 430 mm 400 780 mm 55 420 mm 405 770 mm 70 435 mm 420 785 mm min 0 39 L 2 20 L 13 2 L max 3 38 L 13 7 L 24 7 L 78 5 cm2 314 cm2 314 cm2 1 0 MPa 1 0 MPa 0 7 MPa 0 7 MPa 0 5 MPa 0 5 MPa 300 mm Quick Lock AB SR 2 TAC05 T
7. EE 0 e BioPro e SEC YMC Pack Diol 1 BioStream YO Pilot ee ECO PLUS 4 9 HPLC K Prep 9 DE DAU 9 Siosepar CONTENTS BioProp YMC BioPro QA F SP F YMC BioPro QA SP BioPro SmartSep Q S BioPro BioPro lon Exchange Screening Kit SEC YMC Pack Diol HPLC YMC Triart
8. 1IQ OQ A FE Wl Jill a DA AD 0O DAD DAD 50 DAD 100 DAD 150 DAD 200 DAD 300 DAD 450 DAD 600 DAD 800 DAD 1000 mm 50 100 150 200 300 450 600 800 1000 MPa 10 7 mm 850 mm 100 500 2 10 22 30 80 160 300 600 1200 DAU DAU 50 DAU 100 DAU 150 DAU 200 DAU 300 DAU 450 DAU 600 mm 50 100 150 200 300 450 600 MPa 10 4 mm 700 mm 100 400 2 10 22 30 60 140 250 Bioseparation products catalog so GMP ICHQ7 Q
9. YMC BioPro QA F YMC BioPro SP F YMC BioPro QA YMC BioPro SP um 3 5 CHzNT CHs 3 CHzCHzCHzSOs CHzNT CHs 3 CHzCHzCHzSOs CI Nat CE Nat 0 0 075 0 110 0 230 0 290 0 075 0 100 0 070 0 095 7 DBC gt 12 BSA DBC gt 10 human lgG DBC gt 110 BSA DBC gt 70 human lgG 4 60C pH 2 0 12 0 PEEK BioPro SmartSep BioPro SmartSep Q BioPro SmartSep S um 10 30 R N CH3 3 R SOs pH 2 0 12 0 meq mL resin gt 0 08 mg mL resin DBC gt 100 BSA DBC gt 100 lysozyme BioPro BioPro Q BioPro S BioPro DA BioPro CM um 75 R N CH3 3 R SOs R N CHs 2 R COOH pH 2 0 12 0 3 0 12 0 1 0 13 0 Cn gt 0 10
10. 1 10 8 1 0 mL min 5 uL injection 19 mL min 100 puL injection CCGCTCGAGCTAAAA 8 5 um AAAGCCTGTGTTACC Hydrosphere C185 um YMC Actus Hydrosphere C1 50 X 4 6 mml D 30 mer 50 X 20 mmlLD Recovery Impurities Te I 0 10 20 30 min En Brand 1I1 5 um Brand Il15 um 50 X 4 6 mml D 5OX19 mml D Impurities Ne Recovery 35 l 0 10 20 30 min 7 5 10 12 5 min 2 Bioseparation products catalog 56 purity gt 99 Eluent A 10 mM DBAA pH6 0 methanol 60 40 B 10 mM DBAA pH6 0 methanol 20 80 10 35 B 0 30 min Temperature ambient Detection UVat269 nm Sample synthetic oligonucleotide 100 mol mL di n butylamine acetic acid Hydrosphere C18 1 YMC
11. um 30 75 R NT CH3 3 R N CH3 3 R COOH 1 8 3 0 12 0 0 12 2 0 12 pH 20 120 0 120 1 0 13 0 1 0 13 0 Application 1 Transferrin 0 25 mg mL NE i EE 1 i 2 Cytochrome c 5 mg m 2 Trypsin inhibitor 0 5 mg mL i 2 3 3 Lysozyme 0 5 mg mL BioPro Q75 BioPro S75 1 1 2 3 BioPro DA60 BioPro CM60 D140123A D140123B a 0 5 10 15 20 25 min 0 5 10 15 20 25 min Column 1 mL type 26 X 7 0 mml D Column 1 mL type 26 X 7 0 mml D Eluent A 20 mM Tris HCI pH 8 1 Eluent A 20 mM NaH2P 04 NazHPO4 pH 6 8 B 20 mM Tris HCI pH 8 1 containing 0 5M NaCI B 20 mM NaH2PO4 NazHP 04 pH 6 8 containing 0 5 M NaCl 10 80 B 0 30 min 0 100 B 0 30 min Flow rate 180 cmhr 1 16 mL min Flow rate 180 cm hr 1 16 mL min Temperature 25 C Temperature 25 C Detection UV at220 nm Detecton UVat220 nm Injection 20 ul Injection 20 gt AN 2 Selection Kit BioPro um um Ol Exchange Selection Kit 75 60 1
12. um nm 100 g 500 g 1 kg Triart Prep C18 S 10 12 TAS12S11 120 000 15 TAS12S16 100 000 20 TAS12S21 80 000 Triart Prep C8 S 10 20 TOS20S11 120 000 15 TOS20S16 100 000 20 TOS20S21 80 000 Triart Prep SIL 10 12 TSS12S11 66 000 15 TSS12S16 55 000 20 TSS12S21 44 000 Bioseparation products catalog 26 cGMP 21 5 1IQ OQ CSV BSTP 800 BSTP 03K BSTS 03K BSTS 10K BSTS 30K 800 mL min 3000 mL min 3000 mL min 10 L min 30 L min 0 5 MPa 0 6 MPa 5 30C PFA PTFE EPDM SUS316L PTFE EPDM p
13. 5 000 C18 12 nm 5 000 20 000 C8 OP O 20 nm 20 000 C4 30 nm 5 000 20 000 C18 C8 C4 PROTEIN RP CN 100 000 MW 574 3 465 Hydrosphere C18 12 nm 5 um Brand E2 10 nm 5 um 1 BAM 12P MW 1 425 150 X 4 6 mmlD 150 X 4 6 mmlD NSIM ODS 4 MetE MW 574 ll 4 6 5 D Ala Me E nkephalin MW 588 2 5 4 6 6 y Endorphin MW 1 899 3 7 23 8 7 B En
14. 0 EE 2 m ws EE API 2015 8
15. 99 lgY 0 BioPro Q75 75 R NT CH3 3 QAA0S75 BioPro S75 R SOs SPAO0S75 BioPro DA60 60 R N CH3 2 DAM99S60 BioPro CM60 R COOH CMM99S60 15 Bioseparation products catalog Q S DA CM 4 1 mL 5 mL 2 4 1 mL 26 X 7 0 mmlL D 5 mL 26 X 15 6 mml D BioPro SmartSep Q BioPro SmartSep S BioPro DA BioPro CM
16. 0L YMC BioPro 0 20 40 60 min YMC BioPro QA F 5 um 100 X 4 6 mml D DNA 1Kb DNA ladder 75 12 216 bp mAU Eluent A 20 mM Tris HCI pH 8 1 containing 0 7 M NaCl B 20 mM Tris HCI pH 8 1 containing 1 0 M NaCl 0 100 B 0 30 min Flow rate 0 5 mL min 180 cm hr Temperature 25 C 0 Detection UV at 260 nm Injection 20 puL 0 25 mg mL AM DNA fe CE I YMC BioPro QA F 100 mm 0 10 20 min YMC BioPro QA F 5 um 100 X 4 6 mml D mAU _ NaN3 Lot A lgG4 MAb Eluent A 20 mM Tris HCI pH 8 1 B 20 mM Tris HCI pH 8 1 containing 0 5 M NaCl 10 25 B 0 60 min Flow rate 1 0 mL min 360 cm hr Temperature 25 C Detection UV at 220 nm Injection 14 pL 0 1 mdg mL Sample Mouse monoclonal lgG1 ant human IgG4 5 10 15 20 25 30 35 40 min Purified by DEAE chromatography containing NaN3 P0804
17. DBC 120 BiOPro SmartSep S30 porous S type 30 um 100 B porous S type 30 um 80 9 Column 50 X 5 0 mml D Flow rate 400 cm hr 1 32 mL min E 60 Temperature 25C UO 40 DBC mg mL resin 10 breakthrough 5 um Insulin Lysozyme Human Polyclonal lgG BioPro SmartSep S30 30 73 111 93 0 A porous S type 30 67 72 42 Insulin Lysozyme Human Polyclonal lgG MW 5 800 MW 14 300 MW 150 000 B porous S type 0 9 99 a BioPro SmartSep DBC lgG 2 lgG DBC 120 110 BioPro SmartSep S30 100 A porous S type 30 um 30 B porous S type 30 um 80 E 70 60 Column 50 X 5 0 mm M Equilibration buffer 20 mM citric acid Na0H pil5 3 50 Elution buffer Equilibration buffer containing 0 5 M NaCl 40 Flow rate 200 800 cm hr 0 66
18. rProtein A WE GR EA EE EOE RE 0 4 8 12 16 20 24 28 32 36 40 44 48 min um BioPro SmartSep Q10 10 R N CH3 3 QSAOS10 BioPro SmartSep S10 R SO3 SSAOS10 BioPro SmartSep Q30 30 R N CH3 3 QSA0S30 BioPro SmartSep S30 R SOs SSA0S30 Bioseparation products catalog BioPro 1 H H IH H 0 DBC Dynamic bindi
19. 106 MW l IgM 900 000 2 Thyroglobulin 670 000 3 IgA 390 000 4 Fibrinogen 340 000 5 Y Globulin 158 000 6 Ig9G 150 000 7 Transferrin 75 000 8 HSA human serum albumin 66 000 1 9 oi Antitrypsin 50 000 DioF300 10 0valbumin 45 000 gt 105 11 Carbonic anhydrase 30 000 12 Trypsin Inhibitor 20 100 13 Myoglobin 17 000 Diol 120 14 a Lactalbumin 14 100 15 Ribonuclease A 13 700 16 Cytochrome c 12 400 104 7 3 1 13 1 90 Eluton volume mL Column YMC Pack Diol 300 X 8 0 mml D 5 1 Mi N Eluent 0 1 M KHzP 04 KzHP04 pH 7 0 containing 0 2 M NaCl Diol 120 Diol 200 10 30002 an Temperature 25 lt Detecton UVat280 nm o 1 MW Diol 120 Diol 200 Diol 300 1 Glutamate dehydrogenase 290 000 2 Lactate dehydrogenase 142 000 2 4 3 Enolase 67 000 1 4 Adenylate kinase 32 000 23 5 Cytochrome c 12 400 4 5 Column YMC Pack Diol 500 X 8 0 mml D Eluent 0 1 M KH2P 04 K2HP04 pH 7 0 containing 0 2 M NaCl Flow rate 0 7 mL min Tempe
20. BioStream YMC Pilot ECO PLUS HPLC K Prep DAD DAU Bioseparatig products catalog YMC Pack Diol YMC BioPro 5 000 YMC Triart C18 3 Meteoric Core C18 BIO 3 Wide Pore Columns YMC Pack PROTEIN RP YMC Triart Dio HILIC i 25 YMC BioPro 4 3 YMC Pack Diol YMC Triart C18 phere C18 100
21. C4 30 nm 1 2 1 BSA MW 66 000 2 3 2 Conalbumin MW 77 000 DrODTOI 3 Lipoxidase MW 96 000 Column YMC Pack C4 5 um 30 nm 150 X 4 6 mml D Gradient 30 75 B 0 15 min 75 B 15 20 min Flow rate 1 0 mL min 05011405 D 04093007 D Temperature 37C I MUSEUM RS RR ER NER Detection UV at 220 nm 0 5 10 15 min 0 5 10 15 min A water TFA 100 0 1 A water TFA 100 0 1 B acetonitrile TFA 100 0 1 B acetonitrile 2 propanol TFA 50 50 0 1 C4 30 nm TFA 1 2 2 propanol 1 BSA MW 66 000 C4 30 nm 2 Conalbumin MW 77 000 3 Lipoxidase MW 96 000 Column YMC Pack 5 um 150 X 4
22. Cs 5 10 C8 15 20 C4 C4 CN YMCbasi asic PROTEIN RP 5 4 1 5 5 Web YMC Actus Web nm Triar
23. 2 1 C8 S pH 1 70C pH 11 5 50C 120 120 Triart Prep 100 YMC Triart Prep C18 S 100 YMC Triart Prep C18 S Se i C18 60 60 4 C18 40 nk 20 acetonitile water TFA 10 90 1 70C 20 3 20 0 1 1 1 1 1 0 1 1 1 0 20 40 60 80 100 0 100 200 300 hr hr Column 10 um 12
24. um nm X mm QA F 5 non porous 4 6 X 30 QF00S05 0346WP 110 000 SP F 5 non porous 4 6 X 30 SF00S05 0346WP 110 000 4 6X50 QF00S05 0546WP 120 000 4 6X50 SF00S05 0546WP 120 000 4 6X 100 QF00S05 1046WP 128 000 4 6 100 SF00S05 1046WP 128 000 Bioseparation products catalog o8 YMC BioPro SP 5 um 50X 4 6 mml D AU 0 5 0 20 40 60 min A Dorous S type 1 2 7 um 50 X 4 6 mml D AU i 4 3 N071018F 0 11 rr T T T Lg T Lg T T rr T 0 20 40 60 min 2 3 B 20 mM KHzP04 K2HP04 B porous type containing 0 5 M NaCl 10 um 50 X 5 0 mml D 0 100 B 0 60 min Flow rate 180 cm hr 0 5 mL min for 4 6 mml D ni 0 59 mL min for 5 0 mml D N071018D 0 0 1 3 20 40 60 min YMC BioPro QA YMC BioPro QA 5 um 50 X 4 6 mmlLD Albumin mAU 3 lgG E Transferrin 10 0 N080313E 0 10 20 30 min A porous Q type 10 um 50 X 4 6 mml D
25. HPLC Triart SIL Triart C18 Triart C8 Triart Prep SIL Triart Prep C18 S Triart Prep C8 S um 3 5 10 15 20 nm 12 12 20 12 20 20 17 20 13 a 2 0 10 0 pH 1 0 12 0 20 120 Triart
26. Triart Prep C18 S 10 15 20 20 2 0 10 0 ODS 2 0 12 0 Meteoric Core C18 8 7 ODS C18 2 7 lt Meteoric Core C18 BIO 16 5 ODS Pro C18 1 16 ODS 12 0 8 Hydrosphere C18 12 100 Pro C18 RS C18 8 22 ODS ODS A 3 5 1 0 FE ODS ODS AQ 15 20 50 14 100 5 000 100 000 2 pH Triart C18 1 0 12 0 3 Triart Prep C18 S 2 0 10 0 ODS Triart Prep C8 S 2 0 12 0 C8 Meteoric Core C18 BIO 1 0 10 0 3 ODS A 3 5 10 ODS 15 20 ODS AQ 5 10 15
27. nAU Plasmid pBR322 Hae I digest 8 587 bp 40 Plasmid pBR 322 4 361 bp 20 Eluent A 20 mM Tris HCI pH 8 1 B 20 mM Tris HCI pH 8 1 containing 1 0 M NaCl 10 70 85 B 0 20 min 85 B 20 25 min Flow rate 0 5 mL min UJ Temperature 35 C 9 1 1 0 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 I 1 1 1 1 Detection UM at 260 nm 0 5 10 15 20 25 min MJD A wv YMC Pack Diol 300 Diol 200 5 um 500 X 8 0 mml D X 2 i Plasmid pBR 322 Hae I digest 8 587 bp 40 Plasmid pBR322 _ 4 361 bp 30 20 1 Eluent 0 1 M KH2P04 K2HP04 pH 7 0 containing 0 2 M NaCl Flow rate 0 7 mL min Temperature ambient 25 C 0 P080617A Detection UV at260 nm Injection 10 ul 0 10 20 30 40 50 min DNA 8 587 bp SEC oligonucleotides miRNA YMC Triart C18 3 um 150 X 2 0 mml D 5 DUGG AGU GUG ACAAUG GUG UUG 3 21 nt MW 6890 1 5 DUGG AGU GUG ACAAUG GU
28. YMC Triart C18 100 Hydrosphere C18 Wide Pore Columns YMC Triart Diol HILIC YMC Pack Polyamine I Biostean Pilot ECO PLUS DAD DAU 50 mm K Prep LAB 20 50 mm YMC Actus 20 30 mm K Prep FC DAD DAU 50 150 mm 50 mm K Prep EX DAD DAU 100 600 mm Bioseparation products catalog 02 ib Human serum YMC BioPro QA 5 um 50 X 4 6 mml D a lgG Albumin 10 Transferrin N080313E 0 10 20 30 min Eluent A 20 mM Tris HCI pH 8 6 B 20 mM Tris HCI pH 8 6 containing 0 5 M Nacl 0 30 B 0 15 min 30 100 B 15 30 min Flow rate 0 5 mL min Temperature 25 C Detection UV at 280 nm Injection 20 AL 100 AL mL YMC Pack Diol 300 Diol 200 5 um 300 X 8 0 mml D X 2 Albumin Transferrin N080616A 0 10 20 30 40 50 60 min Eluent 0 1 M K
29. Fc 10 Bioseparation products catalog BSA Tryptic digest of BSA 2 YMC BioPro QA 5 um 50 X 4 6 mml D 20 10 0 N080422B 0 10 20 30 40 50 60 min pa YMC Pack Diol 120 Diol 60 5 um 500 X 8 0 mml D X 2 mAU 10 MW 60 50 10 40 i 30 20 10 10 _N080623L a 0 10 20 30 40 50 60 min YMCbasic 5 um 150 X 2 0 mml D mAU 40 30 20 10 0 NO080318B 0 10 20 30 40 50 60 min Undigested BSA BSA MW 66 000 3 100 20 000 PA Pyridylamino PA Sugar chains Eluent A 20 mM Tris HCI pH 8 6 B 20 mM Tris HCI pH 8 6 containing 0 5 M NaCl 0 15 B 0 30 min 15 60 B 30 60 min Flow rate 0 5 mL min Tempe
30. 2 200 mm UHPLC Bioseparation products catalog 22 mAU mAU 1 4 NH 0 CH 3 NN 3 80 WT 80 ON o N H H 2 3 Cytosine Thymine 60 0 NH 1 W 2 HN nN 40 NS 5 on Sh NH 4 H Uracil Adenine 3 20 3 N HN lt 0 0 Se NH HN 0 4 2 6 B111219E 8 9 8 Mi Guanine Triart C18 100 Column YMC Triart C18 5 um 12 nm 150 X 4 6 mml D Eluent 20 mM KH2P O04 K2HP 04 pH 6 9 Flowrate 1 0 mL min
31. Temperature 37 C Detection UV at254 nm Injection 8 pL 0 01 0 02 mg mL _ Column YMC Triart C18 5 um 12 nm mAU 150 X 2 0 mml D 20 Flow rate 0 2 mL min 151 Gradient 35 50 B 0 84 min 10 0 0 10 20 30 40 50 60 70 F111116H 80 0 Column YMC Triart C18 1 9 um 12 nm mAU 50 X 2 0 mml D 20 lowrate 0 8 mL min Gradient 35 50 B 0 7 min 10 5 S i 0 1 2 3 4 5 6 8 min F 111109K 30 mer 1 5 CMP 2 5 UMP 3 5 GMP 4 5 IMP 5 5 MP 6 5 AMP 3 4 5 6 4 8 10 12 min C130729D Column YMC TriartC18 5 um 12 nm 150 X 3 0 mml D Eluent 20 mM CHsC00H CHsCO0NH4 pH 4 7 Flowrate 0 425 mL min Temperature 25 C Detection UV at260 nm Injection 2 AL 50 9 mL d T 2 2o Eluent A 10 mM di n butylamine acetic acid pH 6 0 B methanol Temperature 35 C Detection UV at269 nm Injection 1 ML 5 nmolmL Triart C18 1 9 um 5 um 1 3 50 mm
32. 100 Sample IEC i 30 min 0 5 10 15 20 25 Eluent 0 1M KH2PO04 K2HP O24 pH 6 9 containing 0 2 M NaCI Flow rate 0 7 mL min Temperature ambient Detecton UVat280 nm Injection mL 0 45 mg lgY SEC YMC Pack Diol 200 5 um 300 X 4 6 mml D lgY 8 Eluent 20 Flow rate 15 0 1M KH2P04 K2HP 04 pH 6 9 containindg 0 2 M NaCl 0 23 mL min Temperature ambient 97 2kDa 66 4kDa 45 0kDa 29 0kDa 20 1kDa 14 3kDa Detection UV at 280 nm Durity 53 5 0 4090824 EE 0 5 10 15 20 min Non reduced SDS PAGE EC Marker SEC YMC Pack Diol 200 5 um 300 X 4 6 mml D mAU 7 5 IgY Eluent a Flow rate Detection 2 5 purity gt 99 0 0 0 Non reduced SDS PAGE 14 3kDa se wa 0 1 M KH2P04 KzHP04 pH 6 9 containing 0 2 M NaCl 0 23 mL min Temperature ambient UV at280 nm e ce x SN gt No ER 97 2kDa 45 0kDa 20 1kDa IsY BioPro Q75 YMC Pack Diol 200
33. 0 0 1 0 2 0 3 0 4 0 min 0 0 2 0 3 0 4 0 min Tri 9 Se MEMS 0 AI _ 2 10 0 1 Triart Prep 0 10 3 1 0 1 0 0 0 0 0 0 1 0 2 0 3 0 4 0 min 0 0 1 0 2 0 3 0 4 0 min RS 2 0 2 0 35 4 1 0 1 0 1 0 0 0 0 0 0 1 0 2 0 3 0 4 0 min 0 0 1 0 2 0 3 0 4 0 min 70 a x 1 0 0 0 0 0 1 0 2 0 3 0 4 0 min um nm 2 g 10 g Triart C18 3 12 TA12S03 28 000 140 000 5 TA12S05 21 000 105 000 Triart C8 3 12 TO12S03 28 000 140 000 5 TO12S05 21 000 105 000 Triart SIL 3 12 TS12S03 11 000 55 000 5 TS12S05 9 000 45 000
34. 2 62 mL min Temperature ambient 25 C 30 Detection UV at 280 nm Sample 1 5 mg mL human polyclonal lgG in equilibration buffer 20 10 0 200 400 600 800 1000 Linear velocity cm hr BioPro SmartSep DBC lgG 2 3 DBC Bioseparation products catalog 99 Purity BioPro SmartSep S30 30 um Recovery Load 45 mg Purity 99 2 Ribonuclease A Cytochrome c Lysozyme Total 1 3 BioPro SmartSep S30 90 9 80 3 99 2 90 6 A porous S type 80 6 59 6 98 3 80 1 B porous S type 72 5 70 2 97 2 80 2 Column 50 X 5 K140819P 1 Eluent A 20 mM NaH2P04 NazHP 04 pH 6 8 B 20 mM NaH2PO04 Naz2HP04 pH 6 8 containing 0 5 M NaCl 0 100 B 0 30 column volumes A porous S type 30 um Flowrate 1600cmhr 5 23 mL min Temperature 25 C 2 Detection UVat220 nm 3 Injection 30 mL 45 mg Proteins
35. 20 DBC DBC DBC Column BlOPro S75 50 50Iml 8 80 Flow rate 800 cm hr 2 62 mL min Equilibration buffer 20 mM Glycine NaOH pH 9 0 lei Elution buffer 0 5 M NaClin equilibration buffer dQ Sample 1 0 mg mL Lysozyme in equilibration buffer a Temperature ambient Detection UV at 300 nm xDBC was determined at 10 breakthrough 20 4 6 8 10 12 14 16 18 20 1 M NaOH 2 3 1 MNaOH 20 10 Column BioPro S75 50 X 5 0 mml D Eluent A 20 mM NaH2P04 NazHP 04 pH 6 8 B 20 mM NaH2PO4 Naz2HP04 pH 6 8 containing 0 5 M NaCl Gradient 0 100 B 0 60 min Linear Flow rate 180 cm hr 0 59 mL min Temperature 25 C t Detecti UV at 220 10 20 30 40 9000MU ha 24 1 Sample 1 Ribonuclease A 2 Cytochrome C 3 Lysozyme 0 5 mg mL BioPro Q75 DBC es EC 2 A E 120 Column BioPro Q75 50 X 4 6 mmlD Equilibration buffer 20
36. 6 mml D Eluent A waterTFA 100 0 1 3 B acetoniTile 2 propanolTFA 50 5070 1 30 75 B 0 15 min 75 B 15 20 min Flow rate 1 0 mL min Temperature 37C Detecton UV at 220 nm 04093007 D T T T T T T 1 2 16 m i Es aE EE 0 4 8 1 1 TT 1 In 05011711 D C4 12 nm 1 2 3 0 4 8 12 16 m 66 000 96 000 C8 C18 20 000 100 000 C4 30nm Bioseparation
37. Albumin mAU 3 ldG Transferrin B Dorous Q type 10 um 50 X 5 0 mmlLD Albumin Transferrin Eluent A 20 mM Tris HCI pH 8 6 B 20 mM Tris HC pH 8 6 containing 0 5 M NaCl 0 30 B 0 15 min 30 100 B 15 30 min Flow rate 0 5 mL min 180 cm hr for 4 6 mml D 150 cm hr for 5 0 mml D Temperature 25 C Detection UVat280nm Injection 20 pL Sample Human serum 100 pL mL og Bioseparation products catalog YMC BioPro SP YMC BioProSP 1 Ribonuclease A 0 5 mg mL 2 Cytochromec 0 5 mg mL 3 Lysozyme 0 5 mg mL Eluent A 20 mM KH2P04 K2HP 04 pH 6 8 pH 6 8 Temperature 25 C Detection UV at 220 nm Injection 20 uL for 4 6 mml D 23 6 uL for 5 0 mml D YMC BioPro QA YMC BioPro QA 5 um 50X 4 6 mml D peaks 50 307 20 10 0 10 20 30 40 50 60 min A porous Q type 10 um 50 X 4 6 mml D mAU Deaks 28 N080417C 0 10 20 30 40 50 60 min B Dorou
38. I 5 um 150 X 4 6 mml D il AL_ 10 20 min G910620A Eluent methanol 20 mM NH4HzP 04 80 20 Flow rate 1 0 mL min Temperature 37 C Detection FLS at x320Mnmaa mg 0 OMNI Injection 3 AL 3 3 pmol mL Sample PA Sugar Chain Series manufactured by TAKARA BIO INC PA HPLC Bioseparation products catalog o4 DNA 1 Kb DNA ladder 75 12 216 bp YMC BioPro QA F 5 um 100 X 4 6 mml D mAU 100 1 Eluent A 20 mM Tris HCI pH 8 1 containing 0 7 M NaCl 50 1 B 20 mM Tris HCI pH 8 1 containing 1 0 M NaCl 0 100 B 0 30 min Flow rate 0 5 mL min J Temperature 25 C CS OA eR Oi EE Detection UVat260 nm 0 10 20 30 ih Injection A TE DNA YMC BioPro QA F 100 mm lt pBR322 ae I Plasmid pBR322 restriction fragments YMC BioPro QA F 5 um 100 X 4 6 mml D
39. nm 150 X 4 6 mml D Column 10 um 12 nm 250 X 4 6 mmlL D Eluent 50 mM tiethylamine in water Eluent acetonitrile water 60 40 0 mM tiethylamine In methanol 80720 Flowrate 1 0 mL min Flow rate 1 0 mL min Temperature 37 C Temperature 50 C Detection UVat254 nm Detecton UV at 254 nm Sample n butyl benzoate Sample caffeine 25 Bioseparation products catalog 0 1 M NaOH acetonitrile 50 50 10CV 12 20 Insulin in human serum ait acetonitrile water 20 80 30CV acetonitrile water 90 10 5CV Column 50 X 4 6 mml D Eluent A waterTFA 100 0 1 B acetonitrile 29 36 B 0 2 min 36 B 2 3 min 29 B 3 6 min Flow rate 1 0 mL min Temperature 25 C Detection UV at 220 nm Sample 10 mg mL bovine insulin human serum 271 Injecton 6 AL 1 CV column volume 2 3CV 1 20 1 0 gt 1 0 gt 1 0 0 0 0 0 0 0 0 0 1 0 20 3 0 4 0 min
40. products catalog 05011708 D C16 30 nm 05011802 D In 0 4 8 12 16 min Condition A MW 500 18 400 Condition B MW 14 300 25 700 AGi6 MW Peak width min 40C 70C AU AU Peak capacity 60 Peak capacity Condition A 21 8 25 8 MPa 1 230 37 0 37 5 MPa 67 1 Oxytocin 1 007 0 017 0 014 3 160 3 740 psi 5 370 5 440 psi 1 2 2 Leu Enkephalin 556 0 015 0 015 40 401 3 B Endorphin 3 465 0 016 40 C 4 Insulin 5 733 0 015 5 B Lactoglobulin A 18 400 0 043 0 030 Condition B 1 Lysozyme 14 300 0 069 0 044 0 F111228B 0 F111226B 2 a Chymotrypsinogen 25 700 0 080 0 049 00 05 10 15 20 25 30 35 mm 00 05 10 15 20 25 30 35 40 minx LactoglobulinA 18 400 0 080 0 048 601 15 8 18 5 MPa 2 290 2 680 psi F111228A mAU 5 Column Eluent Peak capacity 107 2 601 27 4 28 4 MPa 3 970 4 120 psi F111226A 00 05 10 15 20 25 3 0 35 40C 70C Gradient 10 80 B 0 5 min Flow rate Detection Injection System YMC TriartC18 1 9 um 12 nm 50 X 2 0 mml D A water TFA 10070 1 B acetonitrile TFA 100 0 1 condition A B acetonitrile IPA TFA 50 5070 1 conditon B condition A 30 60 B 0 5 min condition B 0 4 mL min
41. 0 000 8 0X300 DL12S05 3008WT 110 000 8 0 Xx 300 DL30S05 3008WT 110 000 8 0X500 DL12S05 5008WT 160 000 8 0X500 DL30S05 5008WT 160 000 20 Xx 300 DL12S05 3020WT 450 000 20 x 300 DL30S05 3020WT 450 000 20X500 DL12S05 5020WT 650 000 20X500 DL30S05 5020WT 650 000 Web Bioseparation products catalog 5 000 WC pH ODS Triart C18 20 100 C18 Triart C18 ExRS 0 45 25 SE C8 Triart Phenyl 17 0 10 r 7
42. 0 psi 2 Two coupled 1 9 pm 100 X 2 0 mml D 30 min gradient 58 1 61 6 MPa 8 430 8 930 psi Column YMC Triart C18 1 9 um 12 nm Eluent A water TFA 100 0 1 B acetonitrile TFA 100 0 08 5 40 B 0 15 min for a single column 5 40 B 0 30 min for two coupled columns Flow rate 0 4 mL min Detection UV at 220 nm Injection 10 pL for a single column 20 uL for two coupled columns Sample Tryptic digest of Bovine Hemoglobin System Agilent 1290 UHPLC 1 9 um Peak capacity 365 Triart C18 P110705A Peak capacity 450 4 15 min P110728A 94 98 10 2 10 6 min 25mAU Peak capacity 630 et 15 min P110707A 8 30 min 70 23 1 9 um 100 mm Triart C18
43. 1 Sample 1 Ribonuclease A 0 5 mg mL 2 Cytochrome c 0 5 mg mL 3 Lysozyme 0 5 mg mL BioPro SmartSep 0 B porous S type 30 um lt 3 K140826P 1 0 ll 2 3 4 5 6 7 min mAU Column 50 X 5 Oml8 Eluent A 20 mM citric acid Na0H pH 5 3 B 20 mM citric acid Na0H pH 5 3 containing 0 5 M NaCI 0 100 B 30 column volumes Flowrate 180 cm hr 0 59 mL min Temperature ambient Detection UVat280 nm Sample Ant hTNF alpha IgG1 Purified by Affinity chromatography Injecton 0 25 mL 0 1 mg IgG1 BioPro SmartSep S30 rProtein A A porous S type 30 um BioPro SmartSep S30 IgG1 Protein A
44. 24A mAU NaN3 MAb Lot B DEAE YMC BioPro QA F 100 mm YMC BioPro QA F SP F 100 mm A 0 5 10 15 20 25 30 35 40 min Bioseparation products catalog YMC BioPro SP F 5 um 100 X 4 6 mml D mAU 3 mAU tR 60 0 min 12 Competitor WCX column 10 um 250 X 4 0 mml D mAU mAU 50 60 70 tR 118 2 min 80 min A 20 mM MES NaOH pH 5 6 B 20 mM MES NaOH pH 5 6 containing 0 2 M NaCl Initial gradient conc 35 B 70 mM NaCD Gradient slope 0 25 B min 0 5 mM Nacl Eluent Flow rate 180 cm hr 0 5 mL min for 100 X 4 6 mml D 0 378 mL min for 250 X 4 0 mml D Temperature 30 C Detecton UV at 2
45. 5 10 15 20 2 5 mg IIS gt 3 RY um nm X mm um nm X mm QA 5 DOrOUS 4 6 X 30 QAA0S05 0346WP 140 000 SP 5 porous 4 6 X 30 SPA0S05 0346WP 140 000 4 6X50 QAA0S05 0546WP 150 000 4 6X50 SPA0S05 0546WP 150 000 4 6 100 QAA0S05 1046WP 160 000 4 6 100 SPA0S05 1046WP 160 000 Bioseparation products catalog gt RS Q S NN 3 30 um 10 um
46. 700 MW ORY 2 Neurotensin 1 672 1 Myoglobin 17 000 3 4 3 Angiotensin I 1 040 104 2 Ribonuclease A 13 700 2 4 Glycine 75 3 Cytochrome c 12 400 1 4 Insulin Bovine 5 700 5 Insulin B chain 3 496 6 a Mating factor 1 684 7 Neurotensin 1 672 103L 8 Angiotensin I 1 296 9 CCK 0ctapeptde 1 143 gt 10 Bradykinin 1 060 Column YMC Pack Diol 60 gt 11 Angiotensin I 1 040 500 X 8 0 mml D 12 0xytocin 1 007 Eluent 0 1 M KH2P 04 KzHP04 13 Angiotensin 931 pH 7 0 14 MetEnkephalin 574 containing 0 2 M NaC 10 lt 15 Leu Enkephalin 9 acetonitrile 70 30 Flow rate 0 7 mL min 16 Tetraglycine 246 Temperature ambient 17 Glycine 75 Detection UV at 215 nm 0 10 20 30 40 min 10 15 20 1 Dio 60 Elution volume mL TIS gt RS S J Diol 60 5 6 4 6 X 300 DL06S05 3046WT 90 000 Diol 200 5 20 4 6X300 DL20S05 3046WT 90 000 8 0X300 DLO06S05 3008WT 110 000 8 0X300 DL20S05 3008WT 110 000 8 0X500 DLO06S05 5008WT 160 000 8 0X500 DL20S05 5008WT 160 000 20 Xx 300 DLO06S05 3020WT 450 000 20 x 300 DL20S05 3020WT 450 000 20 Xx 500 DLO06S05 5020WT 650 000 20X500 DL20S05 5020WT 650 000 Diol 120 5 12 4 6 X 300 DL12S05 3046WT 90 000 Diol 300 5 30 4 6X300 DL30S05 3046WT 9
47. 80 nm Injection ON Humanized monoclonal IgG1 1 mg mL Sample YMC BioPro SP F pH 5 6 YMC BioPro SP F 0 20 40 60 80 120 140 mnn Column YMC BioPro SP F 5 um 100 X 4 6 mml D Eluent A 20 mM NaH2zP 04 Naz2HP 04 pH 6 5 Lot E B 20 mM NaH2P04 NazHP04 pH 6 5 containing 0 2 M NaCl 0 50 B 0 5 30 min Flow rate 0 5 mL min 180 cm hr Temperature 25 C Detection UV at 215 nm Injection 10 AL Lot D Sample monoclonal antibody Il9G1 lk YMC BioPro SP F Lot B Lot A 0 5 10 15 20 25 30 min um nm x mm
48. AC10 TAC15 TAC25 TAC35 TAC50 5 mm 10 mm 15 mm 25 mm 35 mm 50 mm 4 0 MPa 3 0 MPa SR 8 0 MPa 5 0 MPa 5 0 MPa 5 0 MPa 4 0 MPa 2 5 MPa 125 mm 250 mm 500 mm AB 4 40 C SR 16 40 C 1 4 28G fittings 1 4 28G fittings 1 16 1 8 AB SR SUS 316 Bioseparation products catalog 28 HPLC KK Prepe E24 HPLC PC 1Q 0Q CSV K Prep LAB100S K Prep LAB300S K Prep FC750S KPrep FC1500 FC750S K Prep FC1500S K Prep EX03KS K Prep EXO6KS K Prep E EX12KS K KPrep EX25KS K Prep LAB100G eA
49. G UUG U 3 22 nt MW 7196 3 UV mAU 21nt ZZ 09 Eluent A 10 mM DBAA pH 7 5 10 B 10 mM DBAA pH 7 5 acetonitrile 50750 62 72 B 0 20 min 0 Flow rate 0 2 mL min Temperature 30 C PR 0 AM 9 9 PR 0 Detection UVat260 nm and ESInegative mode MS MIC Injection 4 AL 5 nmolmL Int x 100000 Instrument LC Shimadzu Prominence 5 m z 1721 5 m z 1738 1 MS Shimadzu LCMS2020 di n butylamin acetic acid 21 nt lt 2 nt LC MS 5 5 0 FB 10 0 125 150 17 5 min Triart C18 1 A Courtesy of M Yamada SHIMADZU CORPORATION Bioseparation products catalog 1 i EE YMC BioPro
50. H UV 3 BioStream mm 800X900X1360 900 x 1100 x 1800 900 x 1100 x 1800 1200 x 1200 X 1800 2000 x 1500 x 1800 200 kg 250 kg 300 kg 400 kg 600 kg 100V 15A 200V 20A 200V 30A 200V 40A 0 5 MPa 21 5 27 Bioseparation products catalog AV A kd
51. H2P04 K2HP04 pH 7 0 containing 0 2 M NaCl Flow rate 0 5 mL min Temperature ambient 25 C Detecton UV at 280 nm Injection 20 pL 100 AL mL SEC Mouse monoclonal IgG1 anti human IgG4 Purified by DEAE chromatography containing NaN3 2 YMC BioPro QA F 5 um 30 X 4 6 mml D NaNs ImAU Mouse monoclonal lgG1 Anti human IgG4 OO MD WwW WU P080220A Eluent A 20 mM Tris HCI pH 8 1 B 20 mM Tris HCI pH 8 1 containing 0 5 M Nacl 10 25 B 0 18 min Flow rate 1 0 mL min Temperature 25 C Detection UV at 220 nm Injection 10 uL 0 1 md mL a YMC Pack Diol 200 5 um 300 X 4 6 mml D mAU Mouse monoclonal lgG1 40 Ant human IgG4 NaN3 30 20 10 p080530A T T T T T T T T T T T T T T T T T T T T T T T T 0 5 10 15 20 25 30 min Eluent 0 1 M KH2P04 K2HP 04 pH 7 0 Flow rate 0 17 mL min Temperature ambient 25 C Detecton UV at 220 nm Injection 10 uL 0 05 mg mL
52. UV at 220 nm 1 uL 50 ug mL condition A 1 uL 250 ud mL conditlon B Agilent 1200SL min 0 0 05 10 15 20 2 5 3 0 3 5 40 min PC peak capacity 1 gradient tme peak width peak widh 2Wo sh averade MW 0 1 TFA 40 70 2 propanol IPA 10 000 40 condition B FF 7m 40 C 1 9 m 100 X 2 0 mml D 15 min gradient 46 5 48 5 MPa 6 740 7 030 psi 70 C 1 9 pm 100 X 2 0 mml D 15 min gradient 27 6 28 6 MPa 4 000 4 15
53. X 1 mL BPIESKS99 01PK 13 000 BioPro Q75 1 mL BPQAAO0S75 01PK 15 000 BioPro Q75 S75 DA60 CM60 4 75 5 mL BPQAA0S75 05PK 35 000 BioPro S75 1 mL BPSPA0S75 01PK 15 000 BioPro SmartSep 5 5 mL BPSPA0S75 05PK 35 000 BioPro DA60 1 mL BPDAM99S60 01PK 15 000 ui um a 5 mL BPDAM99S60 05PK 35 000 1 mL BPQSA0S30 01PK 15 000 BioPro CM60 1 mL BPCMM99S60 O1PK 15 000 BioPro SmartSep Q30 5 mL BPQSA0S30 05PK 35 000 5 mL BPCMM99S60 05PK 35 000 BioPro SmartSep S30 1 mL BPSSAO0S30 01PK 15 000 5 mL BPSSAOS30 05PK 35 000 Bioseparation products catalog SEC a RS Diol 60 6 1 Diol 120 12 5 000 10 5 5 0 7 5 Diol 200 20 1 Diol 300 30 100
54. bration buffer 160 Detecton UV at 300 nm 0 200 400 600 800 1000 1200 Linear Veloeity cm hr 250 200 Column 50 X 4 6 mml D Flow rate 180 cm hr S for anion exchange resin 6 150 Equilibration buffer 20 mM Tris HCI pH 8 6 Elution buffer 0 5M NaCl in eduilibraton buffer E Sample 1 5 mg mL BSA in equilibration buffer wm Detection UV at 280 nm c 100 for caHon exchange resin BioPro S75 Equilibraiton buffer 20 mM Glycine NaOH pH 9 0 50 Elution buffer 0 5M NaClin equilibration buffer BioPro Q75 Sample 1 5 mg mL Lysozyme in equilibration buffer Detection UV at 300 nm Lot A Lot B Lot C Lot D Lot E DBC BioPro Q S 1a Bioseparation products catalog CIP CIP cleaning in place BioPro 1 M NaOH CIP
55. dorphin MW 3 465 7 Eluent A water TFA 10070 1 B acetonitrile TFA 10070 1 A000313D 209B 0 J Flow ra TO Temperature 37 C Detection UVat220nm 0 5 10 15 20 min 0 5 10 15 20 min 5 000 C18 12 nm Triart Pro ODS Brand E2 1 7 Hydrosphere C18 MW 4 300 17 000 C18 20 nm C8 12 nm 1 04072910 D 04073006 D C8 30 nm 1 04080204 D 04073002 D 1 a EN jma MI EE Ui RN RL CR La T Lt 5 1 amnvml PE ime We LE T 0 5 10 15 min 0 5 10 IS min MW 12 400 MW 5 700 MW 4 300 l Cytoch
56. gt 0 10 gt 0 08 meq mL resin a DBC gt 160 BSA SBC 77 human lgG SBC 90 human lgG DBC SBC 16 Bioseparation products catalog oe 30 mm 100 mm YMC BioPro SP F 5 um 30 X 4 6 mml D AU 0 4 1 3 0 2 1 1 5 mL min 540 cm hr 1 RIDonuclease A 4 8 5 2 MPa 2 Cytochrome c 3 Lysozyme 0 0 N071023K Eluent A 20 mM KHzP04 KzHP04 pH 6 8 5 4 min B 20 mM KHzP04 K2HP04 pH 6 8 containin9 0 5 M NaCIl 0 100 B 0 4 min forYMC BioPro SP F YMC BioPro SP 5 um 50 X 4 6 mmlD 0 100 B 0 60 min forYMC BioPro SP R 3 Temperature 25 C 1 1 lt Detection UV at220 nm 05 Injecton 20 UN ID
57. mM Tris HCI pH 8 6 go Elution buffer 0 5M NaClin equilibration buffer O Flow rate 180 cm hr 0 50 mL min m Sample 1 5 mg mL BSA in equilibration buffer P 40 Temperature 2 Detection UV at 280 nm DBC was determined at 10 breakthrough 0 0 50 100 150 200 7 15 30 60 90 180 days Column BioPro Q75 50 X 4 6 mml D Eluent A 20 mM Tris HCI pH 8 1 B 20 mM Tris HCI pH 8 1 containing 0 5 M NaCl Gradient 10 80 B 0 60 min Linear Flow rate 180 cm hr 0 50 mL min Temperature 25 C Detection UV at 220 nm Injecton 5 20Ml BS UE33 CSS2 Sample 1 Transferrin 0 25 mg mL 2 Trypsin Inhibitor 0 5 mg mL Bioseparation products catalog lgY IEC BioPro Q75 75 um 50 X 4 6 mml D 500 Sample 400 300 200 100 Eluent A 20 mM Tris HCI pH 8 1 B 20 mM Tris HCI pH 8 1 containing 0 5 M NaCIl 10 B 0 15 min 30 B 15 30 min 90 B 30 40 min Flow rate 180 cm hr 0 5 mL min Temperature ambient Detecton UVat280 nm Injection 1 mL 20 mg Protein x Courtesy of Pharma Foods International Co Ltd SEC SEC ss YMC Pack Diol 200 5 um 300X 8 0 mml D
58. ng capacities were determined at 10 breakthrough um meq mL resin mg mL resin under following conditions i Column 50X 4 6 mml D BioPro Q75 75 0 13 183 Flow rate 180 cm hr 3 0 cm min B porous Q type 90 0 19 102 for anion exchange resin Equilibration buffer 20 mM Tris HCI pH 8 6 Elution buffer 0 5 M NaClin equilibraton buffer Sample 1 5 mg mL BSA in equilibration buffer a Detection UV at 280 DBC a 2 um meq mL resin mg mL resin for cation exchange resin Equilibration buffer 20 mM Glycine NaOH pH 9 0 BioPro S75 75 0 12 192 Elution buffer 0 5M NaClin equilibration buffer Sample 1 5 mg mL Lysozyme in equilibration buffer B porous S type 90 0 13 80 Detecton UV at 300 nm BioPro DBC 260 4 BioPro S75 220 B porous S type E A porous S type 200 m A 180 Column 50 X 5 0 mml D Equilibration buffer 20 mM Glycine NaOH pH 9 0 Elution buffer 0 5 M NaClin equilibraton buffer Sample 1 0 mg mL Lysozyme in equili
59. rature 25 C Detection UV at 220 nm Injection 20 1 Myoglobin MW 17 000 2 Insulin Bovine MW 5 700 3 Neurotensin MW 1 672 4 Tetraglycine MW 246 5 Glycine MW 75 Eluent 0 1 M KH2P04 K2HP04 pH 7 0 containing 0 2 M NaCl acetonitrile 70 30 Flow rate 0 7 mL min Temperature ambient 25 C Detecton UV at 220 nm Injection 5 AM Eluent A water TFA 100 0 1 B acetonitrile TFA 100 0 1 5 35 B 0 50 min 35 45 B 50 55 min 45 B 55 60 min Flow rate 0 2 mL min Temperature 37 C Detection UV at 220 nm Injection TE 150 X 4 6 mml D 0 10 20 min G910607A Eluent methanol 20 mM NH4H2P 04 5 95 Flow rate 1 0 mL min Temperature 37 C Detection FLS atEx 320 nm Em 400 nm Injecton 2 uL 3 3 pmolmL Sample PA SugarChain Series manufactured by TAKARA BIO INC YMC Pack ODS A 12 nm 5 um 1 PA Sugar Chain 014 hay netosamine trp agalncto EBEFaHmtGHLRTFY Gr lt i TE HH EC rt HE FT d Fl _ EF 2 PA Sugar Chain 001 tty lactossming WEmB hiantennary Gti Ma Bt Uy ag RAT YO PA lt Fa 3 PA Sugar Chain 002 HN Acetyl lactosaning type triantenry tii l Ma HT HE Er 6 FET De ha Hobo rr TEE FnWW PE gmridmtmmlrnTgrsusel To g Hr He a YMC Pack Polyamine
60. rature ambient Detection UV at 280 nm 1 0 10 20 30 40 mn 0 10 20 30 40 mn DioL200 17 Bioseparation products catalog YMC Pack Diol 300 Diol 200 5 um 300 X 8 0 mmlLD X 2 1 lgG YMC Pack Diol 300 Diol 200 5 um 300 X 4 6 mmlLD X 2 AlIDumin Transferrin mAU _ Human serum N080616A IgM MW 900 000 IgA MW 390 000 FiDrinogen MW 340 000 Y Globulin MW 158 000 N080616E N080616M N080617B N080616F IgG RD MW 150 000 Transferrin N080616C MW 75 000 HSA N080616D MW 66 000 ai Antitrypsin N080616K MW 50 000 Carbonic anhydrase P080528A a MW 30 000 Lysozyme 0 10 20 30 40 50 60 min N080616H MW 14 300 0 10 20 30 40 50 60 min Eluent 0 1 M KHz2P 04 K2HP04 pH 7 0 Flow rate 0 17 mL min Eluent 0 1 M KHzP04 KzHP04 pH 7 0 containing 0 2 M NaCl Temperature ambient 25 C Flowrate 0 5 mL min Detection UV at220 nm Temperature ambient 25 C Injection lOI Detecton UVat280 nm Sample Mouse ascites fluid 60 tmes diluton with water 1 MW 1 Insulin Bovine 5
61. rome c 2 Insulin Bovine 3 Amyloid 8 protein MW 14 100 C8 20 nm 4 Lysozyme MW 14 300 MW 17 000 6 5 a Lactalbumin 4 6 Myoglobin J 5 Column YMC Pack 5 um 150 X 4 6 mml D Eluent A water TFA 100 0 1 B acetonitrile TFA 100 0 1 25 60 B 0 20 min Flow rate 1 0 mL min Termperatue 90 EN 04082002 D UV at 220 nm Detection 00 om 4 300 17 000 C8 20 nm Bioseparation products catalog 20 MW 66 000 96 000
62. s Q type 10 um 50 X 5 0 mmlLD peaks 11 30 N080417G 0 10 20 30 40 50 60 min Eluent A 20 mM Tris HCI pH 8 6 B 20 mM Tris HCI pH 8 6 containing 0 5 M NaCl 0 15 B 0 30 min 15 60 B 30 60 min Flow rate 0 5 mL min 180 cm hr for 4 6 mml D 150 cm hr for 5 0 mml D Temperature 25 C Detection UVat220 nm Injection 20 pL Sample Tryptic digest of BSA BSA Dynamic binding capacity Eluted amount Recovery mg mL resin 10 breakthrough mg mL resin YMC BioPro QA 126 120 95 A porous Q type 73 58 79 B porous Q type 100 35 35 Recovery Eluted amount Dynamic binding capacity X 100 AU Breakthrough curves Column YMC BioPro QA50X 4 6 mml D A porous Q type 50 X 4 6 mmlL D B porous Q type 50 X 5 0 mml D O Linear velocity 180 cm hr A E quilibration buffer 20 mM Tris HCI pH 8 6 Elution buffer 20 mM Tris HCI pH 8 6 containing 1 0 M NaCl mm Detection UV at 280 nm Sample 1 md mL Bovine serum alDumin BSA in equilibraton buffer 0 20 40 60 80 100 120 140 160 BSA mg mL resin YMC BioPro
63. t C18 TA 8 8 Triart C18 ExRS TAR 12 12 Triart C8 TO 16 16 Triart Phenyl TPH 20 20 Triart Prep C18 S TAS 30 30 Triart Prep C8 S TOS YMCbasic 99 Meteoric Core C18 CAS PROTEIN RP 99 Meteoric Core C18 BIO CAW Pro C18 AS um Hydrosphere C18 HS 1 9 SP9 Pro C18 RS RS 2 S02 ODS A AA 2 SQ7 ODS AQ AQ 3 S03 Cs OC 5 S05 C4 BU 10 S11 CN CN 15 S16 YMCbasic BA 20 S21 PROTEIN RP PR 50 S50 9 Bioseparation products catalog Web mm mm 33 H3 1 0 01 4 WT 35 H5 2 0 02 Triart 1 9 um PT 50 05 2 1 Q1 Triart PTH 75 L5 3 0 03 100 10 4 0 04 125 R5 4 6 46 150 15 6 0 06 250 25 10 10 YMC Triart C18 1 9 um 100 X 2 0 mml D TA 12 SP9 10 02 PT L TA12SP9 1002PT ll
64. wrate 1 0 mL min Temperature 37 C 1 0 km 12 nm Detection UV at 220 nm Injecton 10 pL 0 167 mg mL TAS12S 11 01 0 0 2 5 5 0 7 5 10 0 12 5 15 0 17 5 min Triart Prep C18 S 10 um 15 um 20 um Triart C18 1 9 um 3 um 5 um MW 4 300 17 000 i 2 34 1 Cytochromec MW 12 400 YMC Triart Prep C18 S 5 2 nsulin bovine MW 5 700 10 12 nm A 3 Amyloid protein MW 4 300 Jrm 2 nm 5121214A 4 Lysozyme MW 14 300 1 4 5 a Lactalbumin MW 14 100 6 Myoglobin MW 17 000 3 6 2 5 Column 150 X 4 6 mml D Trl Eluent A water TFA 100 0 1 YMC Triart Prep C8 S B acetonitrile TFA 100 0 1 10 um 20 nm J 25 60 B 0 20 min S121214B Flow rate 1 0 mL min Temperature 37 C Detection UV at 220 nm 00 20 40 6 0 80 10 0 12 0 14 0 16 0 18 0 min Injection IOM 12 nm Triart Prep C18 S 20 nm Triart Prep C8 S 1
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