T4- FITC
Contents
1. TQ Prep Multi Q Prep CD4 T4 T4 T4 CD4 T T CD4 gt CD4 1 1042. 1 12mm x 75mm 2 100xL 3 10L X MSlgG1 FITC 10L 4 10 12 5 TQ Prep Multi Q Prep 6 25 35 EPICS 7 2 2 2 4 2 8 C
3. No Wash No Wash Q Prep TQ Prep Q Prep TQ Prep T4 CD4 DiGeorge SCID AIDS AIDS HIV HIV CD4 CD4 CD4
4. T4 T8 CD4 19 65 T4 EPICS PROFILE S PLUS IV T4 FITC n 8 Min Max Mean 1SD TA 39 56 47 6 T4 mm 594 1 649 1 069 369 T4 CD4 CD4
5. T CD4 488nm FITC Tm 4 FS 90 SS 1 2 3 d 1 1 12
6. EDTA 6 909
7. 6 1 2 8 C 2 20 25 C 3 RD1 FITC N T4 FITC 6603862 50 0 5mL 1 2 10 11 12 13 14 16 17 18 19 20 21 22 23 24 McMichael AJ ed Leukocyte Typing Ill White Cell Differentiation Antigens 1987 Oxford Oxford University Press p 202 206 Morimoto C Letvin NL Distaso JA Aldrich WR and Schlossman SF 1985 The isolation and characterization of the human suppressor
8. 1 TQ Prep Multi Q Prep 7546999 300 TQ Prep Multi Q Prep 3 A B C 2 1 6603152 300 1mL PBS 24mL 9 259 6 2 PBS PBS 6603369 1 500mL pH 7 2 0 2 3
9. 6603862 2009 11 2006 9 20200EZY00030000 877449 T T4 FITC w FITC Fluorecscein isothyocyanate 1 T4 FITC 0 01 lt 0 1 g T4 CD4 62kD CD4 8096 T 609
10. MslgG1 FITC T4 FITC FB 6603864 50 0 5mL EDTA 1 100L 1 1 2mL 3 10x10 mm 10x10 mm 3x107mm Q Prep PBS 20 25C 6 a gt 10 x 10 mm 10 20 x 10 2 20 30 x10 3 30 40 x10 5 40
11. Fc MSlgG1 FITC 1 29 6 29 T T Harvard Medical School Dr S F Schlossman Coulter Immunology T B
12. B CD20 85 100 3 96CV 596 1 0 19 3 4 5
13. 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460 135 0063 5 7 TOC BECKMAN COULTER 4 4 6603862 0911
14. Q Prep ik 2 CD4 Flow Count LH 700 mm mm x x 10 CYTO TROL 6604248 Fc Mo2 RD1 KC56 FITC CD14 CD45
15. 24 2 1 2 100L 10 xL Xt MSlgG1 FITC 10L 45 PBS 2 3mL WMA THELL 400 450 x g 5 PBS 25 1mL 30 2 250u L Anz STU PBS 2mL 400 450xg 5 9 11 PB
16. CD4 RAHTI CD4 T T T B T CD4 Class l MHC T4 SFCI12T4D11 CD4 ERRI T BALB c 2 AO R LRAR RR U v 77 A NS 1 AG4 lg lgG1H kL T4 FITC FITC Fluorescein isothyocyanate FITC 5 lt 10 468 509nm 504 541nm 1 0 5mL BSA 0 2 0 01M NaCl 0 15M NaN3 0 1 T 1 4
17. CD4 T4 T8 SLE CD8 TAIT8 SLE CD4 CD8 SLE 3 4 CD4 T4 T8 SLE SLE CD4 CD8 SLE T4 T8 TA T8 T4 T8 CD4 CD8
18. 3 T T CD4 T CD8 2 T CD4 TA CD4 T
19. 60 x 10 6 60 100 x 10 10 100 200 x10 20 b lt 3 x 10 mm 1 25C 500xg 5 2 LH700 5 10x10 mm 1 100 u L 3 4 1 Q Prep TQ Prep Multi Q Prep Coulter Immunology 3 A B C TQ Prep Multi Q Prep
20. T4 T8 CD4 CD8 CD4 CD8 TA T8 T T T47T8 T4 T8 CD4 ARC AIDS related complex AIDS AIDS CD4 T4 T8 0 CD8 TA T8 HRU CD4 CD8 MS SLE T4 T8 CD4 CD8 MS EF SLE
21. S 500u L EPICS 1 2 Q Prep EPICS EFS Q Prep 0 5mL 3 FITC Log 1 CD4 3 4 5 6 7 8 9 10 11 12 13 14 1
22. ctive systemic lupus erythematosus J Rheumatol 16 1315 1319 Sato K Miyasaka N Yamaoka K Okuda M Yata J and Nishioka K 1987 Quantitative detect of CD4 2H4 cell in systemic lupus erythematosus and Sjogren s syndrome Arthritis Rheum 30 1407 1411 Ramos EL Turka LA Leggat JE Wood IG Milford EL and Carpenter CB 1989 Decrease in phenotypically defined T helper inducer cells T4 4B4 and increase in T suppressor effector calls T8 2H4 in stable renal allograft recipients Transplantation 47 465 471 Pedrazzini AS Freedman AS Andersen J Heflin L Anderson K Takvorian R Canellos GP Whitman J Coral F Ritz J and Nadler LM 1989 Ants B cell monoclonal antibody purged autologous bone marrow transplantation for B cell non Hodgkin s lymphoma Phenotypic reconstitution and B cell function Blood 74 2203 2211 Reinherz EL Kung PC Goldstein G and Schlossman SF 1979 A monoclonal antibody with Selective reactivity with functionally mature human thymocytes and all peripheral human T cells J Immunol 123 1312 1317 Reinherz EL Haynes BF Nadler LM and Bernsteln ID eds Leukocyte Typing II Human T Lymphocytes 1986 New York Springer Verlag p 8 9 Koepke JA and Landay AL Precision and accuracy of absolute lymphocyte counts Clin Immunol Immunopathol 1989 52 19 27 135 0063
23. inducer T cell subset J Immunol 134 1508 1515 Morimoto C Letvin NL Distaso JA Brown HM and Schlossman SF 1986 The cellular basis for the induction of antigen specific T8 Suppressor cells Eur J Immunol 16 198 204 Reinherz EL Meuer SC and Schlossman SF 1983 The delineation of antigen receptors on human T lymphocytes Immunol Today 4 5 8 Reinherz EL Morimoto C Fitzgerald KA Hussey RE Daley JF and Schlossman SF 1982 Heterogeneity of human T4 inducer T cells defined by a monoclonal antibody that delineates two functional subpopulations J Immunol 128 463 468 Meuer SC Schlossman SF and Reinherz EL 1982 Clonal analysis of human cytotoxic T lymphocytes T4 and T8 effector T cells recognize Products of different major histocompatibility regions Proc Natl Acad Sci USA 79 4395 4399 Reinherz EL Cooper MD and Schlossman SF 1981 Abnormalities of T cel maturation and regulation in human beings with immunodeficiency disorders J Clin Invest 68 699 705 Schmidt RE 1989 Monoclonal antibodies for diagnosis of immunodeficiencies Blut 59 200 206 de Martini RM and Parker JW 1989 Immunologic alterations in Human Immunodeficiency virus infection A review J Clin Lab Anal 3 56 70 Fauci AS 1988 The human immunodeficiency virus infectivity and mechanism of pathogenesis Science 239 617 622 Taylor MGJ Fahey JL Detels R and Giorgi JV 1989 CD4 percentage CD4 number and CD4 CD8 ratio in HIV infectio
24. n How to choose and how to use J AIDS 2 114 124 Morimoto C Hafler DA Weiner HL Letvin NL Hagan M Daley J and Schlossman SF 1987 Selective Loss of the suppressor inducer T cell subset in progressive multiple sclerosis Analysis with anti 2H4 monoclonal antibody N Engl J Med 316 67 72 Reinherz EL Weiner HL Hauser SL Cohen JA Distaso JA and Schlossman SF 1980 Loss of suppressor T cells in active multiple sclerosis Analysis with monoclonal antibodies N Engl J Med 303 125 129 Weiner JL Hafler DA Fallis RJ Johnson D Ault KA and Hauser SL 1984 Altered blood T cell subsets in Patients with multiple sclerosis J Neuroimmunol 6 115 121 Smolen JS Chused TM Leiserson WM Reeves JP Alling DW and Steinberg AD 1982 Heterogeneity of immunoregulatory T cel subsets in systemic lupus erythematosus Am J Med 727 783 790 Smolen JS Morimoto C Steinberg AD Wolf A Schlossman SF Steinberg RT Penne E Reinherz EL Rerchlin M and Chused TM 1985 Systemic lupus erythematosus dellneation of subpopulations by clinical serologic and T cell marker analysis Am J Med Sci 289 139 147 Morimoto C Reinherz EL Schlossman SF Shur PH Mills JA and Steinberg SD 1980 Alterations in immunoregulatory T cell subsets in active systemic lupus erythematosus J Clin Invest 66 1171 1174 Raziuddin S Nur MA and Alwabel AA 1989 Selective loss of the CD4 inducers of suppressor T cell subsets 2H4 in a
Download Pdf Manuals
Related Search