概要 必要な試薬と装置 塩化アンモニウム溶血剤を用いた直接免疫蛍光
Contents
1. 38 0 EDTA3K BD 367660 FALCON 15mL BD 352099 FACS Lysing Solutiton 10X 12. 15 FACS EDTA3K 20 25
3. CD11b CD16 Clinical Applications of Flow Cytometry Quality Assurance and Immunophenotyping of Peripheral Blood Lymphocytes Tentative Guideline Villanova PA National Committee for Clinical Laboratory Standards 1992 NCCLS document H42 T Procedures for the Collection of Diagsnostic Blood Specimens by Venipuncture Approved Standard Villanova PA National Committee for Clinical Laboratory Standards 1991 NCCLS document H3 A3 47
4. 0 196 2 FBS PBS 8C 9 FALCON 126x75mm BD 352058 10 BD 11 0 19 PBS 196 28C 1
5. 39 40 20 25 1X 14mL 15mL 1mL FACS Lysing Solution FACS Lysing Solution 3 5 3 300xg 5 4 5 5mL 300x g 5 8C 6 1mL 7 9096
6. FITC 1 125x75mm 50 L 100 L 3 30 4 2 GmL 5 200xg 5 6 7 196 PB5S 0 5mL 8C 8 FACS
7. L NH4CI 89 9g KHCOs 10 0g EDTA4Na 370 0mg pH 7 3 2 8C 1X 10mL 10X 90mL 20 25C 1 Vortex 0 196 PBS Dulbecco PBS pH7 0 2 PBS 0 2 m 8C
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