IO テスト PE 標識抗体 CD2 IOT11a
Contents
1. IO PE CD2 IOT11a 2 80 12 IO PE CD2 IOT11a A07744 1002. 1 4 m Awa Mk Ficoll Paque 8
3. CD2 HPB ALL 1 JURKAT 2 90 CD2 KM3 3 19 1 HPB ALL 2 JURKAT 3 KM3 Non T Non B PBS 4 CD2 HPB ALL JURKAT 3 10 3 5 5 1 gt 0 19
4. CD58 LFA 3 T 39C1 5 T11 1 39C1 5 IgG2a PHA PE Phycoerythrin 486 575nm 560 590nm T CD2 T E PE IO EDTA 6
5. 1 25 C 500 x g 5 2 LET 3 FOIE ES 4 LH700 5 PBS 5 10 x 10 mm 1 12mm x 75mm EJ 2 SROAREIEN 1004L 3 20xL IOTest Rat lgG2a PE 20L 4 15 5 6
6. T B NK IO
7. PBS 2mL MHLET 7 400x 9 3 8 7 8 9 0 5 1mL 0 1 0 59 Kan PBS 10 24 3 Ficoll Paque 1 3 4mL PBS 2 Ficoll Paque 4mL 1 3 2 8 C 400 xg 30 4 Ficoll Paque 2 BEE M22O fll DIB ERKE Ar I RAY 5 PBS 2 8 C 400 xg 8 KEIL PBSEMATKHHLET 2 8CC 400 x g 4 K
8. 8 24 FS 90 SS 2 4 PE Log 2
9. 2 EPICS XL CHE vs PE Lymphocytes Leukocytes Count 58 75 188 25 188 1888 1 1 166 1888 18 18 PE PE CYTO TROL 6604248 Fc DR FSERLE BERD HAIER II xt IOTest CD45 FITC CD14 PE A07738 I IM1201 IOTest
10. 2mL 1 Sprent Vaikus L Bierber B E Bogart R E Wolff 1 H Burkakoff S J Functional analysis of CD2 Mab reactivity 1989 Leucocyte Typing IV White Cell Differentiation Antigens W Knapp et al Eds Oxford University Press p 274 277 Sewel W A Brown M H Dunne J Owen M J Crumpton M J Molecular cloning of the human T lymphocyte surface CD2 T11 antigen 1986 Proc Natl Acad Sci USA 83 8718 8722 Hannet I Erkeller Yuksel F Lydyard P Deneys V De Bruyere M Developmental and maturational changes in human blood lymphocyte subpopulations 1992 Immunol Today 13 215 Crawford K Gabuzda D Pantazopoulos V Xu J Clement C Reinherz E Alper C Circulating CD2 Monocytes are Dendritic Cells 1999 J Immunology 163 5920 5928 J lymphocytes and the thymus 1989 in Fundamental Immunology 2nd edition Paul W E Ed Raven Press 69 93 Ball E D Foon K A Immune markers in hematologic disease 1991 Crit Rev Onco Hematolo 11 267 Olive D Ragueneau M Cerdan C Dubreuil P Lopez M Mawas C Anti CD2 Sheep red blood cell receptor monoclonal antibodies and T cell activation Pairs of anti T11 1 and T11 2 CD2 subgroups are strongly mitogenic for T cells in presence of 12 0 tetradecnoylphorbol 13 acetate 1986 Eur J Immunol 16 1063 1068 Haynes B F S
11. h 2009 11 A07744 2006 9 20200EZY00111000 I T IO PE CD2 lOT11a 1 2 3 4 1 IO PE CD2 IOT11a PE IgG 20L CD2 50kD CD2 T E T 9596 NK B
12. 18 IM38515 67 5uL 0 59 PBS 3 A09777 IM3648 VersaLyse IM1400 OptiLyse B A11895 A11894 OptiLyseC A07799 IB IM3514 IOTest 3 IOTest Rat lgG2a PE IM1272 100 2mL Ficoll Paque Phamacia 17 0840 03 a 2 1 100L 5x 10 mm 10x10 mm PBS 3x 103 mm3 93
13. 10 11 1 20 ux ee ae 1 PBS PBS 6603369 1 500mL pH 7 2 0 2 BSA 0 5 0 1 PBS VersaLyse PBS 1mL IOTest 3 A07800 I IM3515 12 5L 0 19 PBS VersaLyse PBS 1mL IOTest 3 A07800
14. EIL PBS 2 8 CC 400 x g 3 PBS 5x10 A mm3 5 x 10 mL 90 A Nee 6 7 8 9 0 1 12mm x 75mm 2 Ficoll Paque 5 x 10 5x 10 mL 100L 3 20 L IOTest Rat lgG2a PE 20 L 4 2 8 C 30 5 PBS 2mL 2 8C 400 xg 5 6 5 7 0 5 1mL 0 59 PBS
15. ummary of T cell studies 1986 in Leukocyte Typing Il White Cell Differentiation Antigen Rheinherz E L et al Eds Springer Verlag p 3 30 3 4 135 0063 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460 135 0063 5 7 TOC econ COULTER tE Na IMMUNOTECH A BECKMAN COULTER COMPANY 130 avenue de Lattre de Tassigny B P 177 13276 Marseille Cedex 9 France A07744 1109 4 4
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