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J5-RD1

1. CD10
2. CD10 non T 8096 40 509 B B non T CD19 Ficol paque 7 Fc
3. 0 1 2 8 C 2
4. T non T J5 RD1 non T non T ALL CD10 CD10 Common Acute Lymphoblastic Leukemia Antigen CALLA 100kDa I CD10 B J5 RD1 CALLA non T T CD10 non T 80 40 50 nodular lymphocytic lymphoma Burkitt s lymphoma T lymphoblastic lymphoma
5. 4 5 200L IgG MslgG2a RD1 6 4 C 830 7 4 C 400 xg 5 8 3 4 9 PBS200L 4 C 400 xg 5 3 4 PBS 200L EPICS Cytomics 10 3 4 1 1
6. 2 PBS 8 0g L 2 12 2 9g L 1 0 2g L 0 2g L pH7 2 7 4 BSA 0 196 0 196 3 MSlgG2a RD1 6602888 100 Propidium lodide Calbiochem 53705 0 01mg mL 0 05mg mL a Acridine orange Baker A366 3 0 005mg mL oo Ficoll paque Pharmacia 17 0840 03 1 EDTA 3 gt 10 x 103 L 10 x 10 L 3x10 L
7. CD10 CD10 959 296 J5 CD10 10 42 J5 RD1 CD10 J5 RD1 y 0 96X 4 49 r 0 989 n 52 0
8. 2 Harvard Medical School Dr S F Schlossman FAB TCR lgH
9. 2 19 6 2 1 J5 RD1 Ficoll paque 200L PBS 109 6 20L 2 1 3 CD10 x 100 Fc
10. 90 a 11 1 x 10 1x10 wL 1mL 12 PBS 4 C 400 x g 4 13 0 05mg mL Propidium lodide 3 1 14 PBS 4 C 400 x g 4 15 14 16 859 6 b 11 25 000 1 x 10 L 25 L 12 Propidium lodide 0 01mg mL 10 x L 13 30
11. Ficoll paque 5 Ficoll paque
12. PBS 2 4 15 25 6 a gt 10 x 10 L 10 20 x10 2 20 30 x10 3 30 40 x103 5 40 60 x10 6 60 100 x10 10 100 200 x10 20 37 PBS b 3 x 10 L 1 25C 500 x g 1 700rpm 5 2 3 4 LH 700 10 x 10 wL 1
13. 488nm RD1 FS 90 1 4 SS 1 EDTA 6 90 85 Ficoll paque
14. BSP NBS PBS 10 000 L PBS 10 000 lt J5 RD1 gt 1 5L 1 200L PBS BSA0 19 NBS 29 0 19 BSA NB
15. 20 25 C 3 J5 RD1 6603475 100 1 10 Ritz J Pesando JM Notis McConarty J Lazarus H Schlossman SF 1980 A monoclonal antibody to human acute lymphoblastic leukemia antigen Nature 283 583 Ritz J Nadler LM Bhan AJ Notis McConarty J Pesando JM Schlossman SF 1981 Expression of common acute lymphoblastic leukemia antigen CALLA by lymphomas of B cell and T cell lineage Blood 48 648 Clavell LA Lipton JM Blast RC Kudish M Pesando JM Schlossman SF Ritz J 1981 Absence of common ALL antigen on normal bipotent myeloid erythroid and granulocytic progenitors Blood 58 333 Sallan SE Ritz J Pesando JM Gelber R O Brien C Hitchcock S Coral FS Schlossman SF 1980 Cell surface antigens Prognostic implicatton in childhood acute lymphoblastic leukemia Blood 55 395 Greaves MF 1981 Monoclonal antibodies as probes for leukemic heterogeneity and hemopoetic differentiation In Leukemia
16. 2009 11 6603475 2007 2 20900AMY00062000 877429 CALLA J5 RD1 IN ww 1 Common Acute Lymphoblastic Leukemia Antigen CALLA 100KD CD10
17. Acridine orange 0 005mg mL 10L 3 14 15 100 85 1 1 12mm x 75mm 2 100wL 3 IgG MslgG2a RD1 4 45 5 a b a
18. ae COULTER 6603475_0911
19. 2mL 5 15 b PBS 400 x g 5 1mL 30 2 250L 6 PBS 7 4 C 400 xg 5 8 9 PBS 10 EPICS Cytomics 2 Ficoll paque 1 12mm x 75mm 2 Ficol paque 1 x 10 1x10
20. L 1mL 3 4 C 400 xg 4 4 200L IgG MslgG2a RD1 5 4C 30 6 PBS 1mL 4 400 xg 4 7 6 2 8 PBS EPICS Cytomics 3 Ficoll paque 1 1 x 10 200L Ficoll paque 200 L 96 2 4 C 400xg 5 3
21. 100 L 5 2 Ficoll paque 1 4mL PBS 2 Ficoll paque 4mL 1 3 400 xg 30 4 Ficoll paque 5 PBS 4 400xg 8 6 PBS 4 C 400 xg 4 7 PBS 4C 400xg 3 8 PBS 1 x10 wL 1 x 10 mL 9 Fc 10 15 7 1mg mL 10 a b
22. T CD10 B B J5 CALLA T ALL BALB cJ NS 1 AG4 lg IgG2a H z L J5 RD1 Phycoerythrin PE PE Protein 1 lt 2 468 575nm 568 590nm 1 0 5mL 0 2 0 01M 0 15M 0 19 CD10
23. Markers ed W Knapp Academic Press p 19 Greaves MF 1981 Biological basis of differential prognosis In immunologically defined sub classes of acute lymphoblastic leukemia In Leukemia markers ed W Knapp Academic Press p 463 Nadler LM Ritz J Todd RFII Griffin J Reinherz EL Schlossman SF 1981 Diagnosis and treatment of human leukemia and Ilymphomas utilizing monoclonal antibodies Progress in Hematology ed E Brown Grune and Stratton Inc NY p 187 Cossman J Neckers LM Leonard WJ Greene WC 1983 Polymorphonuclear neutrophils express the common acute lymphoblastic leukemia antigen J Exp Med 157 1064 Hokland P Ritz J Schlossman SF Nadler LM 1985 Orderly expression of B cell antigens during the in vitro differentiation of nonmalignant human pre B cells J Immunol 135 1746 Boucheix C Perrot JY Mirshahi M Giannoni M Billard F Bernadou A Rosenfeld C 1985 A new set of monoclonal antibodies against acute Ilymphoblastic leukemia Leuk res 9 5 597 604 4 4 11 12 13 14 15 16 17 Boucheix C Perrot JY Mirshahi M Bernadou A Rosenfeld C 1983 A rapid method for detection of membrane amtigens by Fluorescent microscopy and its application to screening hybridoma antibodies J Immunol Methods 57 145 150 Lebien WT Mac Cormach RT 1989 The common acute lymphoblastic leukemia antigen CD10 Emancipation from a functional Enigma Blood 73 3 625 635 Penchansky L
24. Pirrotta V Kaplan SS 1993 Flow cytometric study of the expression of neutral endopeptidase CD10 CALLA on the surface of newborn granulocytes Mod Pathol 6 414 418 Connelly JC Chambless R Holiday D Chittenden K Johnson AR 1993 Up regulation of neutral endopeptidase CALLA in human neutrophils by granulocyte macrophage colony stimulating factor J Leukoc Biol 53 685 690 Taki T lda K Bessho F Hanada R Kikuchi A Yamamoto K Sako M Tsuchida M Seto M Ueda R Hayashi Y 1996 Frequency and clinical significance of the MLL gene rearrangemants in infant acute leukemia Leukemia 10 1303 1307 Heerema NA Maben KD Bernstein J Breitfeld PP Neiman RS Vance GH 1996 Dicentric 9 20 p11 q11 identified by fluorescence in situ hybridization in four pediatric acute lymphoblastic leukemia patients Cancer Genet Cytogenet 92 1 11 115 Ganju RK Shpektor RG Brenner DQ Shipp MA 1996 CD10 neutral endopeptidase 24 11 js phosphorylated by casein kinase Il and coassociates other phosphoproteins including the lyn src related kinase Blood 88 4159 4165 135 0063 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460 135 0063 5 7 TOC
25. S 1 1 2 1 6603152 300 PBS 24mL 1mL 2 1L 8 26g 1 0g EDTA4 2 37mg pH7 2 7 4 1 1 2 30 2 10 15 20 30

J5-RD1

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