1. 2 9 2 B201 029 852 9351 FAX O20 4623 561 1 E mail info rizo co jp http www rizo co jp Copyright e201 1 RIZO Inc All Right Reserved 2. 6 DNA DNA 70 1 1 WV TE 7 3 CpH8O 1 1 25 24 1 SIGMA Cat No P2069 1 5 m 3. DNA DNA DNA PCR DNA DNA DNA 1OOO PCR DNAFTNTFON E 40C 2 FE PowerClean DNA Clean up Kit DNA FF0 E DNA 4. 2 DNA DNA 1 3 A B 0 8 Agarose 0 5XTBE DNA ON DNA PCR 16S RNA 723bp DNA 250 1 M 100 200 400 500bp 1 2 3 A 4 B 2 PCHR Kuske et al App Environ Microbiol 64 77 2463 72 1998 DNA FF0 E A 5. DNA FF0 E DNA 1 5m 1 DNA E 900 1 500 uu1 50 100mg 500D 15 000 rpm 10 7O0 ul 300u1 15 000 rpm 10 500 gu 50Ogl 2OC 10 15 000 rpm 10 400g1 7O0 15 OOO rpm 1 30 5O DNA DNA DNA DNA DNA DNA 6. DNA FF0 E DNA 1 DNA 00 E 90041 5OOx 1 2 100 ul1 50 100mg 500 1 1 3 4 15OOOrpm 10 7OOl 5 1 1 v v 3OO 1 6 15 000 rpm 10 7 50O gl 1 5m 1 8 20C 20 9 15 OOO rpm 10 7. 10 3 7O09 40Ow 11 15 OOO rpm 10 12 DNA FF0 E 13 3O1 TE DNA DNA 2 DNA 8. DNA DS OOO8 DNA Ver 1 0 RIZO Inc DNA DNA MA E DNA DNA DNA PCR DNA 9O mL 100 20 25 28C 3OC