単核細胞の間接免疫蛍光染色 概要 試薬と装置
Contents
1. 45 7 BD 1 gg 8 9 0 196 PBS 1959 PBS 28C 12. 10 FACS 46 1z CPT CPT
3. 1 CPT BD 3G62753 Ficoll Paaue Ficoll Paque FALCON 126x75mm BD 352058 3 FALCON 96 U BD 353918 4 5 BD 6 0 1 2 FBS PBS 2 8 PBS
4. BD 2 1g Fc
5. 30 45 8C 200x gg 9 100 L B00x g 3 30 45 mL BC 300x g 5 30 45 BC 200x g 3 100L 800x g3 800 L 196 PBS
6. PBMC CPT 20 25C 4 PBMC FicolL Paque 1 6O0 L 30 uL Ou L 12x75mm 2 x 107 mL 9096 3 1 75mm 50 L 1X10
7. 300L 19 PBS 12x 75mm 30 45 mL 300x 8C 5 0 5mL 196 1 10 mL 2 8C FACS Parks DR Lanier LL Herzenberg LA Flow cytometry and fluorescence activated cell sorting FACS In Weir DM Herzenberg LA Blackwell C eds Handbook of Experimental Immunology Oxford Blackwell Scientific Publications 1986 chap 29 Jackson AL Warner NL Preparation staining and analysis by flow cytometry of peripheral blood leukocytes In Rose NR Friedman H Fahey JL eds Manual of Clinical Laboratory Immunology 3rd ed Washington DC American Society for Microbiology 1986 226 233 47
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