IO テスト PE 標識抗体 CD20 IOB20a
Contents
1. IO o CD20 N2. 7 8 2 8YC 12 IO PE CD20 IOB20a IM1451 100 2mL Braylan R C Orfao A Borowitz M J Davis B H Optimal number of readents reduired to evaluate hematolymphoid neoplasias results of an international co
3. O 1 2 1 20 ux 1 PBS PBS 6603369 1 500mL pH 7 2 0 2 BSA 0 59 0 1 2 PBS VersaLyse PBS 1mL IOTest 3 A07800 12 54L 0 19 PBS VersaLyse PBS 1mL IOTest 3 A07800 67 5L 0 59 PBS 3
4. B9E9 IgG2a B PE Phycoerythrin 486 575nm 560 590nm CD20 CD20 PE 1 IO 2 EDTA 3 6 4
5. A09777 VersaLyse IM1400 OptiLyse B A11895 OptiLyse C A07799 IOTest 3 4 IOTest Mouse IgG2a PE A09142 100 2mL 5 Ficoll Paque Phamacia 17 0840 03 2 1 100L 5x103 mm3 10 x 103 mm PBS 3x 103 mm3 1 25 C 500 xg 5 2
6. EPICS XL vs PE Lumphocutes Leukocutes Count 48 68 88 28 1 1 18 108 18088 1 18 108 1008 PE PE CYTO TROL 6604248 Fc IOTest CD45 FITC CD14 PE A07738 A a NO TRF A A EE T B NK
7. 24 FS 90 SS PE Log 2 2
8. IM1451 Saeco COULTER I B IO PE CD20 IOB20a 1 2 3 4 IO PE CD20 IOB20a PE IgG 2012 10 1 20200EZY00111000 CD20 35 37kD CD20 B pan B B CD19
9. PBS 5x10 mm 5 x 106 mL 11 90 1 12mm x 75mm 2 Ficoll Paque 5 x 10 5 x10 mL 100 L 3 20 L IOTest Mouse lgG2a PE 20gL 4 2 8 30 5 PBS 2mL 2 8 C 400 x g 5 6 5 7 0 5 1mL 0 59 PBS 8
10. 3 4 DxH800 5 PBS 5 10 x 10 mm 1 12mm x 75mm 2 100u L 3 20xL IOTest Mouse lgG2a PE 20L 4 15 5 6 PBS 2mL 7 400 xg 3 8 6 7 9 0 5 1mL 0 19
11. 5 Ficoll Paque 8 9 10 11
12. 0 59 PBS 10 24 3 Ficoll Paque 3 4mL PBS 2 Ficoll Paque 4mL 1 3 2 8 400 x g 30 4 Ficoll Paque PBS 28 C 400 x g 8 PBS 2 lt 8 400 xg 4 PBS 2 8 C 400 x g 3 10
13. alm 6 1 Raii 2 959 CD20 JURKAT 3 0 5 1 Nim 6 pre B ALL 2 Raji Burkitt lymphoma 3 JURKAT T cell Leukemia PBS 4 CD20 Nalm 6 Raii 3 109 3 5 5 1 0 19
14. nsensus meeting 2001 Cytometry 46 23 27 Jenning C D Foon K A Recent advances in flow cytometry Application to the diagnosis of hematologic malignancy 1997 Blood 8 90 2863 2892 Rosenberg S A Classification of lymphoid neoplasms 1994 Blood 5 84 1359 1360 Zhou L J Tedder T F CD20 Workshop panel report 1995 Leucocyle Typing V White Cell Differentiation Antigens Schlossman S F et al Eds Oxford University Press 511 514 CO O oo OO ID 135 0063 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460 135 0063 5 7 TOC IM1451_1210
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