PrimerArray® Analysis Tool Ver.2.2
Contents
1. RNA Housekeeping Gene Primer Set 3790 3791 3792 deNorm manual http medgen ugent be lt jvdesomp genorm geNorm_manual pdf Accurate normalization of real time quantitative RT PCR data by geometric av eraging of multiple internal control genes Vandesompele J De Preter K Pattyn F Poppe B Van Roy N De Paepe A Speleman F Genome Biol 2002 Jun 18 3 7 RESEARCH0034 Epub 2002 Jun 18 http wwwr takara bio coJp 6 6 Fold Difference 3D profle2. Microsoft Offce Excel 2007 2 OK Microsott fficg ActiveX ctiven HL ctieeg 1 TE 0 URL FEF FF DO array calculatinn tonl wd2 0x ee ed 2 http wwwr takara bio coJp 10 http wwwr takar
3. lt 1 PCR dBi 0 4 6 B HO 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 2 4 6 8 1D 12 14 16 18 20 22 24 26 28 30 32 24 36 38 40 Cycles Cycles Threshold PCR Log scale Primary Curve 2 4 BB 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 C
4. IO EU a EF3 EGF EGFR IERBB2 IAKT1H AKTS IFIGF IFO5 IPIKSRS APPLYT IGSK3BH IHPRT1 1 MG aPC_ BIRCS IKBKB TRE JAK1 UN KRHS RHO8 SMD2 SHMRD3 PGK1 _ 16 FLDT ICYCS MAPKT MAPKE MAP2KT BAD RACYT R IRAD51 RAFT PRALA GWPDH 1 ES EE ET F ST 18 ea ITCP7L ITGFA TGFBT TGFB2 TGFB3 TGFBR1 TGFBR3 TF 3 BM 19 NT TCFTLT CASPS CASFS PIKSRS IIRKBRG AERHGEFs ob al 93 2 iaog9999 55 a op 10 000 00 29 1 000 00 REGG pathway ad 3n 100 00 1 39 EE 100 F 3 ara Bin is gm Hzmd eerie FT RE 3 010 Te 36 37 OOdi H 38 39 4H http wwwr takara bio coJp 8 KEGG_pathway KEGG KEGG pathway hsaWS nlnrertal cancer hsall 1J Fancreatic Camrer iefinstion ot rde color when behavior ts nalyred A Up Gene Point Black pcmepomt Be Red be wo 1 L
5. IdRaRa PrimerArray Analysis Tool Ver 2 2 V201212 PrimerArray Analysis Tool Ver 2 2 PrimerArray PH001 PH015 PN001 PN015 1 PCR Ct AACt PrimerArray Analysis Tool Ver 2 2 Microsoft Offce Excel OS5 Mi crosoft Ofce Excel Windows XP operating System Microsoft Offce Excel 2003 Microsoft Offce Excel 2007 http www takara bio co jp primerarray_tool 1 Ct PCR Ct PCR
6. cutoff Ct cutoff value http wwwr takara bio coJp 5 5 Normalization Factor set sample data Normalization Factor normalization_factors HKG 1 NF value Normalization Factor AA n Te Control sample Test ample uantity ratin 64SE 08 138E 07 535ECmW 249E07 SgiE0W 1mE 07 EC mm J1 CE el J1 CC 8 8 3doEOm 168E09 15eE mm 114E09 0 547E 0 346E 07 1 DOE DDE 1 J ET 3 1 Normalization Factor HKG
7. Fold Difference Control Sample 1 Test Sample ce co GO 1 CO O 18 Scatter plot Control Sample Scatter plot LDLE RT Scatter Plot expression level SD Well Symbol Sample Sample Sample Sample AKT3 2 127E 02 3 CDk4 a FRSF10H BR RALBP1 TestSamhls 1 E 04 1 E 03 1 E 02 EU 1 E 00 1 E 01 1 E 02 ControlSample http www takara bio co jp 7 3D Profile Fold Difference Plate Test Sample Fold Difference Symbol Fold Difference 0 5 0 5 2 2 A E F H J K RLL Fr Nl ER Cr ai _ r 7 RIP JHE T1 EK 1 33E H1 BS5E 0 14EH90 iE MMHG 335EO1 595E1 1 y 4 BGE 01 EN 2SEHHO 1 32E 1
8. mm IA1O 3348 3395 3383 13 RAT 2363 2362 23722 14 GUSB 2 2387 2376 2404 15 IE2E3 9 315g 3154 stal 16 ESE 02m 2469 2509 2539 17 EGRR B03 so78 3145 311 18 ERBB2 94 2611 2618 2618 _19 Akm 2844 2848 2866 _ 20 kT2 B06 25s4 2589 2598 21 ee 07W 25 28105 N 4 Lil Plateselect Platelnfo Gere CanrtrnEampleData JstSampleData PCR amp eff lt rorrralization factors scatter http wwwr takara bio coJp 4 4 Test Sample Test Sample TestSampleData Control Sample Ct set sample data clear Ct cutoff Ct cutoff Ct 35 Ct 35
9. a bio coJp 1 1 IdRAR4 Tel 0 543 6116 Fax 0 5483 19 http www takarga Dio CO jp V201212
10. ay Plate Select PlateList Product Code Product Name mate soreet rayr Focal acdhesson Human Primer Aray Colorectal Cancer amp Pancreatic Cancer Huma ay Lyio cyto ecepior nterectont io WP 91Ur3 CE EGGG_ cytoto 3 Primer dray T aCeptor sigralmg pathwav Mouse p Frosta1e ancer snome MOuse Pr imer ray Diabetes mellitus Typel amp Typell Mouse 3 Control Sample Plate Select Control Sample ControlSampleData exp1 C exp2 D exp3 E Ct PCR Q amp 10 2 4 RC EE EGG we 1 Symbol well Control Samp 3 AKTa 04 2616 2645 2657 _4 GDPK4 02 265 2656 2655 5 Repke IAA03 2839 2843 2849 6 TNFERSFIOB Ao4 2051 2058 2056 3111 3095 3104 06 2256 2241 2252 10 GTNNB1 A08 33s9 3392 3436 11 pec IA09 2236 2235 2259 12
11. c eaereeer ms dsplayed in map of KEGG patheay Coierbal mr Ho acims buen EE 1 ELLE re Ei en St et eB EE Fn 2 EE oh a ne EE A NE a a Et Es 1 ET PEPS era FP arm ET A EE Th TL LT ETE rt Etiam Fa arm mm Nl as Fh le Ti Fm ra mp kuu an pilid sh pe a Fun ii Ra IE EL 1 EE LR Em LET a LL be ET hE 1 EF a I epi CF ee Fm HL Ampemm 1 aka ee 6 F 1 Tal rm ee el EE La 2 FF aln RI LL rm TT Fe LE 1 Er ms EE TO WW a H Ap LE 1 La LIL vv ki Se hl BMP TestSampleData clear Plate http www takara bio co jp 9 PrimerArray Analysis Tool Ver 2 2
12. ycles Threshold http wwwr takara bio coJp 2 2 Ct Ct PCR 3 Ct Microsofte Office Excel CSV PCR PCR Omit PrimerArray Analysis Tool Ver 2 2 http wwwr takara bio coJp 9 I PrimerArray Analysis Tool Ver 2 2 A ACt 1 PrimerArray Analysis Tool Ver 2 2 PrimerArray Analysis Tool Ver 2 2 PrimerArray Analysis Tool Ver 2 2xls 2 Plate PrimerArr
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