リグノフェノール誘導体によるダニアレルゲン低減化
Contents
1. Siri Reya IE AMARE No 31 2007 NDUUUOUDDUUDULUUDLUUUDLLUUDDL OLD 000000000000000000000000000000 BT ep IE HELLE nini Allergen Deactivation by Lignophenol Derivatives Partll Kazuaki MASUYAMA Naomi NISHIKAWA Atsuo FUNAKI Hiroyuki IOKA and RieMIHARA The mite allergen deactivation by ligno p cresol LC lignopyrocatechol LPC and lignopyrogallol LPG were investigated All these lignophenol derivatives deactivated mite allergen effectively The deactivation order of Derfl and Derf2 was LPG gt LPC gt LC The deactivating effect of LPG was similar to tannic acid Poly 4 vinyl phenol well deactivated Derf1 allergen like LPG LPC and tannic acid but didn t deactivate for Derf2 A clear difference was not seen in the deactivation capacity between solvents that dissolved the samples of LPG and LPC Since tannic acid is water soluble once tannic acid soaked fabrics were washed tannic acid couldn t keep to the fabrics the deactivation effect was almost lost On the other hand it was revealed that LPG well adsorbed BSA bovine serum albumin like a tannic acid when LPG solution and BSA solution were mixed When the adsorption and the precipitation between BSA and LPG was observed it was thought that deactivation mechanism of mite allergen was caused by denaturing protein Key words mite allergen ligno phenol derivatives tannic acid poly 4 vinyl phenol a AEN HBP hillu B L ESE EE t E ni ODO BOB DO hili haidh2. DO DO ODO BOBO ODO ODO DO ODO DO0 DO 0 24pAa000000 000000000000 JUD Derflo 00 PBSOO 8 3x 10 Www O poeaDOo 0750100080000 0000 30 OD OD UDO DUO 0De2 00000000000 II ODO BOBO O DUDO DO DO ODO DO DL ODO BOBO ODO DD ODO ODO DO DO ODO DO DD OD OOO 100 400000 ODO DO ODO DD ODO ODO OD LO HOO 000000 H gg dust 1 0 1 2 0 8 3 27 4 8 0 HO 300000000 400000000000 Dado gg o gg O da Dg OD Dodo 00000 pad 000000 40000 000000000 DA4Bo gg o gg th DO DD 00000000 DAaBo gg T DD 00000000 Daga gd ong gogo 000000000700 Dago go og og ooo 000000 HOOUOU Dertl L Derfl NO 0000000 ELISA 0000 LCD HOOOUOQUOOO Dri 000000000 0 1em OO al YODO 0 gg 0000000 2emx 2cm JOD DO DEE 3 1x 10 ww0 0 0 D O00000 impo DU 6 gg 0 000000 OOOO Derfl 0000000 ELISA 000800 SU YDBO DD 0d 000000 415nm 0 000 AAA ANO bi ii UNE AA NO iH HOUUU YUUUUUUUUOUUUOU HOU DABOa gg o 0D Dd O 0d 0000000000 DA4Bo gg o Dg gd 0d DO DD 00000000 hBi Py gg o at ty DD 00000000 Dado gg og Dg 0d DO DD 00000000 Aa Bog o gn o od e a 20 EEE EEE DODO0DO0D00 00 0 000000000000 DODO0DO0 0000 DD 00000000000 ODO DO DDD DO DO O 00 D0D00O IL l 0000000 0000000000000 40 C DODO0D0O0D0D0 00 0 000000000000 DOdO0D0000000De 2p0000000 aaa phi m 311 D tit Phi E e DD Oh AOADODODO0D OL DOBO0 0000000 0 0 000000000 MATE MAEA 00000000000 HO LPG QOUUYUOUUUUUUUUUUOOU OO Deri DOADODODOOO BSAUUUOO EU pa aa WE NEAR EN 4000000000000 1 5mD g 800 000000000 3 wwpg LPG J 0000 3004 10 PBSO 0 0
3. J 0 1 wwg BSA HOU Derti 00d00000000000000 O O O 10 000rpm 100000000000 00 AAA E ANN UU E A OOD UUOUUUUUUUUUUUY U Dert1 20 00 0000000 0 200 300 Derf2Q 00 Dberfip000060 UUUUUUUDULULUUUULUUULUULUUL UUUUUUUUUDUULUU 20000 30000 Aaa A ERBE EI p4vp amp k II U 0 U 000000000 8000 p4vp20k IT 0 40 0000000000 20000 Ac UUUOO Mel UUUOO EtU OU d0000 Derf2 UUUUUDUUDUDUUUDUUULDLLD UOUUUQUGUUU ULPG gt Lec gt LOCQOUUUUUU eB a E A a a UUUUUUUDULUDUUUULUUULUULUUL UODOUOUOUDLCEOUPp amp PUDUUUDUUULDLLD UUUUUUUUUDUULULU 2500 400000 HOUTAQ LPGUUUDUUDDUUDUDULD 200 UNUUUUUUUDULUUUULUUULUULUUL 000 O od E RAF RARA T A IE FAN No 31 2007 Evaluated value by Dan Scan N N G ww w an om SH Control p4vp Sk Ac a p4vp Sk Me ES p4vp Sk Et s p4vp 20k Ac MEE p4vp 20k Me nna p4vp 20k Et Ez 5 TA Et EEE LC Me KzZERIIIIINI2 LC Et es LPC Me EEE LPC Et mu Ml n T D D R TEE lt lt DPEPPPIT SRE 35H 0 2 Derf2 IUUDDDUDUDUUUUuDUDUuuD HOUOUOU UI BL DODOoDo U 2 03 OD00000000000000000000000 w UJUDUDUU S90 UUUUDUUULDUUUUOULDUUUUOUUDUL 20000 U U O LPCULPGUUUUU E 00060 UUUUUUUUOULUUUULUUULUULUUL UUUUUUUDULULUUUDULUUULUULUUL 0000 UODELISAUUUUUD Defil 000000 sSUUUUUUUULUUUUUUUULUULLUND DOO OO00000000000000000 DADODo0 DD 000000 20 Derf2 0 000 HOU TAQ LPGQOUOUUUUUUUUOO Derfi 0 4 De
4. ODO DDD DO DOo0LO IO A OA a 0 a DOpOaDbo0 DO DO DO DD 0000000 00L P2gg00000000000000 0 0 LPGI pp 480000 000000000000 DADO 000000000000 ODerf20 000 ODO BOB DO DO ODO DDD 000 DoLo DAdBg god 000000000000 DerfA ODO BOB DO DO ODO DO ODO00Do0D ODO BOB DO DO ODO DO O DD0DOoD A A ANN Wwhitley I 0D800090900000000000 UUUUUUUUULUDUULUDULUUUUUUULL UUUUUUUUDULUDUULUDULUUUUUUULL DODO BOB DUH EH PH TID ITP TY Il U UUUUUUUUULUDUULUDULUUUUUUULL HOUUUUUUUUUUUOULPGQUUUUOOU dApo0000000000000 BSAN U Dert IDO DDD O DDD OD DDO IU l DOBODO DO DO DO DO DO UO odo 00d UUUUOUUUUULUUUN aaa E aaa i o hh DADO BOBO ODO ODO DO ODO DO DO 0000 10000 Lignocellulosics Sorption of phenolic compounds Treatment with 72 sulfuric acid lt Excess water LIL Insoluble lt wsshing Lignophenols U1 DUDDUOUUUDLDUUUUULLLN od HI pgg000000000000 TAD p4vp 0 O 8000 Y 200000 000000000000000 ODO BOBO ODO ODO DO ODO DO DO 00400000000 805600 0 0 Y Li O ODerf1 HOU Derf2 0 0000000000000 l UU a da Derf2 IL Derf2 OD O Derf1 50ug O O50010 I D HOUUUUUUUUUUUUUUUUUUUUO 0 254100000000000 0 01 w p 0 O s E a UI HOOUUOUUUUUUUUEU PBS pH 7 40 000 gH et P EEE U0O000 Dert2 00 000 p4vpl TAQ LCO LPCH LPG O 2 w TI U La UOUUOU OOO Derfl zga e A IE FAN AERE No 31 2007 HOUUUODUUUUE 20m O O 20cmx 15cm HOUUUUUUUU s 000000000 30 C ODO BOBO ODO ODO DO ODO
5. DO UL dop0000 Derfl 000 BSApo0000060 HOUUUUUUUUUUU LPG TAULU LD NUUUUUDUUUDUUUUUUDUUUL HOUUO HOUUGUULCOOUG Derf2QU0U00000000 TAQ pavpHOOUUUUUUDOUOUUUUUUL HOUUUUUUUUUUUUUUUUUUUUU HOUUUOKDOUUUUUDUODUULCOUUODUUUYT HOUUUUUDUODOUU LPC0 0 LPG00000 Derfl 0 O0 Derf2 40d 00 0000000060 DODBO0D 0D ODO 0000 DOBODO DO DO DO OOO DO0DODO OL N Derfi Der 0 00 LPGO LPCO LC O O O DBOBDoODDODOO LPGY 71400000 O UUUOUUOUUL O0 p4vp O00000 Der 000000000 HOUUGUUOOD Derf2 0000 TAQ LPC EPGODOD O DD OOO ODO OD 00000 UVHOUUUUUUUUUUUUUUUUUUUUU DBABDO DO OOOO DO ODO ODO Derfl ER e A IE FAN No 31 2007 centrifuge 6 EPGODDODODODO BsAg DO Deri 0000000000 JUD U l DO 0 LPGO BSA 2 LPG D Derf1 3 0 0 TAD BSA 40 0 TAQ Derf1 Derf2Q 0000000000000 l DADO BOO DO DUO DO ODO DO Doo DADO BOBO OD DO DO DUO DOD ODO DADO BOB DO DD ODO DO DDODODO DODO BOB DO DD ODO DO DDODODO DADO DO DD O DDD 23 0 LPGO TADO OD Oo Doo o0000 000 O0 Derft 0O00 BSAQVOUUUUUUUUDUOL ID DO DO DLPGI BSA LPGO Derf1 TA BSAUUUUDDUUDDUUUUUDUUDULDLD UUUDUUDUUDUUULDUUUUDULUUL UUUUUULUULULUUULUULUUULUU UUUUUUUULUD UOUU 1 J A Woodfolk et al Chemical treatment of carpets to reduce allergen A detailed study of the effects of tannic acid on indoor allergens J allergy Clin Immunol 94 p 19 26 1994 2 T Hashimoto et al Accumulation of Group I Delmatophagoides farinae and Stability of the Mite Allergen inthe Cultured Medium of All
6. ergenicity Bull of JESC 28 2001 3 T Suzuki et al Control of Mite Allergen by Anti Allergen Agent Grafted Cloths Extended Abstracts of The 33rd Textile Research Symposium 99 2004 4 E Tovey et al Changes in mite allergen Derp1 in house dust following spraying with a tannic acid acaricide solution Clin Exp Allergy 22 67 74 1992 5 M Labieniec et al Interactions of tannic acid and its derivatives ellagic and gallic acid with thymus DNA and bovine serum albumin using spectroscopic method J Photochem Photobio B 82 72 78 2006 6 M Funaoka et al Characteristics of Lignin Structural conversion in a Phase Separative Reaction System Composed of Cresol and Sulfuric Acid Holzforcshung 50 p 245 252 1996 ODO BOBO O DUDO DO DO ODO DO DO UU
7. f goado 00000000000 000000 Derf2QHO0U00U0UUUU0UU y 30 UOUFAQOUUUU Acl UU Up4vp O UO 8000 OOOO LOOUUUU MefUUUpsvpH 000 lt O O E E O Der f1 inhibition N A Ot O 4 00 O oD O O o oD p4vp 8k C C C C C C C C C C C C C C C CT 21 ER e A IE FAN No 31 2007 4 0 EA Before washing l After washing 9 0 3 0 2 0 2 0 1 5 1 0 Evaluated Value by Dani Scan Cont LC Ac LCMe LC Et LPC Ac LPC Me LPG Ac LPG Me LPG Et 05 pagpBg00080000000000000000 Derf2 UU pvp TAY LPC LPG II OUOUUUUU 80 UODOUUDDUUDULUDUDUUDULCEUDULDD 10 U UUUUUUUUUUUUOLU LU Up4vp UO OO Derf20 000000 Dert OU 000000 pApo00000 00000000 Werfl Derf2 HOUUUODUUY 25kDa 14kDaQ O I 000 DO OB DOD ODO DO DO DDO0DDO O00 HUBUHDUDUDDUUDUUUUDDUHUI 0 20 Def20 0 0000000000000 HOUU TAILPCII III LPGI 000000060 HOUUUUUUO Derf2OOO0U0U0UUUUU0OU DApoa0000 000 SU TAppo000 DADO DO DD ODOO 2000000000 DO OB DO DD ODO MODO oDO 3 0 UU 400 00000 DO DD ODO DDO DO DADO BOBO O DUDO DO ODO DODO HO TAUUUDUUDUDUUDUUDUUULDLLD UUUUUUUDULULUUUULUUULUULUUL UUUUUULUUOULUUUULUUULUULULUL HOUUU MeUUULUUUUULLDUOUUULLD UUUOUUUOUUUOUULUUL O00 UUOUUUUUUUUUULULU UUOUUUUUN UUUOUUUDUUUDUUUDUUUOU SULLLUL HOUUUUOUUU LPGO TAUDDDUUDDODD 22 UUUUUUUUOULUUUULUUULUULUUL OOOO O LPG PSAI LPG Derf1l TA BSANpPp0000000050 UU TAQ Derf1 PODODO DO DO
8. i T i ll UUUUUUUUULUDUULUDULUUUUUUULL a AA A A U EB UUUUUUUUULDUULUDULUUUUUULUL ODO DO DD Do doo 60009 0 00L BR I A Ae UUUUUUUUULUDUULUDULUUUUUULL UUUUUUUDUUUULUULUULUUULUULLN AUNAR ib Pi hi ih iii I HUUOUUO UU UW OUUUUUOULD UW UUOUUUUDUUL OB 0 UUUUOUUUU F Phintu EEE UUHUHUOUUUUUUUUUUUUUUUUUO UUUUUUUUDULUDUULUDULUUUUUUULL UUUUUUUUULUDUULUDUULUUUUUULUL UUUUUUUUUUUDULUUULUUL UDULU D UUUUDUUDUUUUDUUUUDUUUUDUUULUND Aaa taa Bda Bao ODO BOB DO DO ODO DDD DO0ODO0D DODODODO ppe eE EN au Ot gn ear ODO BOB DO DO ODO DO TT 1 D poapo0000000006 q10000 0006 DODO BOB DO DO ODO DO ODO DOoD ODO BOB DO DO ODO A E EE zga e A IE FAN AERE No 31 2007 UUUUUUUUDULUULUUUUDULUUUULL UODOUOUDDUUUDUDUL 10u g g fine dust ODO DO DO DO ODO DD O DDODO0D DADO BOB DO DO ODO DO DDD OoLD ODO BOB DO DO ODO DO DDD DOoD ODO BOB DO DO ODO EERE EEN R ODO BOB DO DO ODO DDD DDODOoD HUUUUU ODO DO DO DO DDD DO Doo IS a Ba shpesh pipi papi OOOOOOO00dd00 o000000 HUUUUUUOU OOOO Tovey QOOOO000 7400000 UUUUUUUUULDUULUDULUUUUUUULL UUUUOUUUDUOULULUUULULUUOUULLUUUL Pavpl UUULULUUUUDUULUUUUUUDULUN UUUUUUUUDULUDUULUULUUUUUUL DOO OO0O0000000000000000 AAA AA ANA A A p444g00000000 001ec000 000 ODO BOB DO DO ODO DDD DO00DoLo Derf27 Y 000000000000 pvp 0050 ODO BOB DO DO ODO DDD EE EE Labieniec POD OD ODO DD00D 0000 DADO d 0D 0D UI DNAD OO B SAO BD BOBO OD BOBO DO 0000000 ODO BOB DO DO ODO DDD DDD o0D AO A A Aa ODO BOB DO DO ODO DDD DDD E ODO BOB DO DO
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