遺伝子組換えキット 取扱説明書ver2.1
Contents
1. NT OO OO POV 42. DNA 42 1 DNA DNA DNA DNA 1929
3. A A 2 ATP 008
4. Part 3 2 10 10 140 10 1 2
5. 5 5 20 14 1 lt 1 DNA DNA
6. Feel so Bio TEL 03 627 7 6041 FAX 03 627 6042 Mall info feelsoDio net FAX FAX http www leaveanestcom 160 0004 Ad 135 0016 2 2 20 RS 2 8 5F TEL 03 5632 9610 FAX 03 5632 9619 TEL 03 6277 8041 e mail mail cosmobio co jp EE LU FAX 03 6277 8042 URL http www cosmobio co jp
7. 8 9 7
8. 121 C 20 4 C LB DNA 4 1 2
9. 2 3 4 5 lt 6 7
10. 210uL 1 DNA 10 L 1 1 LB 250uL XxX2 1 LB 1 60 L 30 2 DNA DNA DNA
11. J M109 20 C DNA DNA 20C 20 C 100mg mL LB 20 LB 20C LB 1 500ml 1 100mL
12. 2 3 DNA 4 DNA 7 4 4 1 5 1 2 1 P1 P1 1
13. DNA PCR Feel so Bio19 003 2 1 DNA DNA DNA Feel so Bio19 004 DNA 15 3 2 DNA
14. 250 gL 10 3 2 2 LB 37C LB LB 2 1 1 LB LB LB ee 37 C
15. DNA DNA DNA DNA DNA DNA Feel so Bio19 004 DNA DNA DNA 4 D NA L DNA DNA
16. DNA DNA DNA DNA Feel so Bio19 005 DNA a 6 2 16 2 19
17. DNA ATP DNA Part 1 1 LB 2 5 LB LB 1L 500mL 121 C 20 60C 1 2 6 LB 1 2 LB gt LB 250 ux
18. 20 25L 1 DNA 120L 1 10 150L 1 500pL 1 1 2mL 1 LB 100mL 1 LB 500mL 1 20 1 50 5 50 2 3 C 1 42 1 1 20 L 5 1 200 kL 5 1 6 1 5 1 1L 300mL 1 500mL 1 _ 4 Escherichia coli M109 LB
19. 42 C 50 _12 5 4 2 42 C 50 6 LB 250 ux 10 7 6 100 LB Amp a 6 LB Amp LB Amp LB LB Amp 8 37C 4 4
20. 7 8 DNA DNA 7 DNA 1 1 1
21. 14 LB AmDp LB Amp LB s LB Am p 2 709 1 2 4 10 LB LB 300mL 100mL 121 C 20 3
22. LB 5 Part 2 1 DNA 22 L 5 210 ux L 5 LB 250 L 10 x 1 LB Amp x 2 DNA22 gL 210 ux L LB 250 L x 1 1 1 100 L 2 1 DNA 10 gL 3 2 10 4
23. 1 100 006 Feel so Bio 19 oO5 ver 2 1 NN ES NE nS get Close to Biotechnology 2 3 4 5 6 6 7 9 10 14 1 15 16 2 17 DNA GFP UV
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