IO テスト PE 標識抗体 CD8 IOT8
Contents
1. 2 EPICS XL vs PE Lumphocutes Leukocutes 3 4 5 Count 4 2 12 28 1 1 1 CYTO TROL 6604248 Fc IOTest CD45 FITC CD14 PE A077382. 1IO CD8 HPB ALL 1 CCRF CEM 2 90 CD8 KM3 3 1 1 HPB ALL 2 HPB MLT 3 CCRF CEM HPB MLT PBS 4 3 10 3 5 5 1 0
3. IOTest T B NK
4. IO PE CD8 IOT8 A07757 100 2mL 1 Hannet 1 Erkeller Yuksel F Lydyard P Deneys V DeBruyere M Developmental and maturational changes in human blood lymphocyte subpopulations 1992 Immunol Today 6 13 215 218 2 Alcover A CD8 cluster report 1995 Leucocyte Typing V White Cl Differentiation Antigens Schlossman S F et al Eds Oxford University Press D 353 354 3 Clark P Normansell D E Innes D J Hess C E Lymphocyte subset in normal bone marrow 1986 Blood 6 67 1600 1606 4 Baume D M Caligiuri M A Manley J Daley J F Ritz J Differential Expression of CD8a and CD8b Associated with MHCRestricted and Non MHC Restricted Cotolytic Effector Cells 1990 Cell Immunol 131 352 365 5 Moebius U Kober G Griscelli A L Hercend Meuer S C Expression of different CD8 isoforms on distinct human Ilymphocyte subpopulations 1991 Eur J Immunol 21 1793 1800 Rothe G Schmitz G Adorf D Barlage S Gramatzki M HOffkes H G Janossy G Knuchel R Ludwig W D Nebe T Nerl C Orfao A Serke S Sonnen R Tichelli A WOrmann B Consensus protocol for the flow cytometric immunophenotyping of hematopoietic malignancies 1996 Leukemia 10 877 895 7 Nicholson J K A Hearn T
5. 1 25 500 x g 5 2 3 4 DxH800 5 PBS 5 10 x 103 mm3 1 12mm x 75mm 2 100L 3 20 L IOTest Mouse lgG1 PE 20L 4 15 5
6. 10 11 ND 1 2 1 20 ux L 1 PBS PBS 6603369 1 500mL pH 7 2 0 2 BSA 0 5 0 1 2 PBS VersaLyse PBS 1mL IOTest 3 A07800 12 5L 0 19 PBS VersaLyse PBS 1mL
7. 4 5 Ficoll Paque 8 9
8. T 80 CD8 NK B9 11 CD8 cw B9 11 IgG1 HLA A2 CTL PE Phycoerythrin 486 575nm 560 590nm T CD8 T PE 1 IO 2 EDTA 3 6
9. 6 PBS 2mL 7 400 xg 3 8 6 7 9 0 5 1mL 0 5 PBS 10 24 3 Ficoll Paque 1 3 4mL PBS 2 Ficoll Paque 4mL 1 3 2 8 C 400 x 30 4 Ficoll Paque 5 PBS 2 8 C 400 x g 8 6
10. 5 7 0 5 1mL 0 19 0 59 PBS 8 24 FS 90 SS PE Log 29
11. PBS 7 2 8 400 x g 4 8 PBS 9 0 2 8 C 400 x g 3 10 PBS 5x103 mm 5 x 106 mL 11 90 1 12mm x 75mm 2 Ficoll Paque 5 x 10 5 x 10 mL 100L 20 uxL IOTest Mouse lgG1 PE 20wL 2 8 30 PBS 2mL 2 8 C 400 x g 5 6
12. IOTest 3 A07800 67 5uL 0 5 PBS 3 A09777 VersaLyse IM1400 OptiLyse B A11895 OptiLyse C A07799 IOTest 3 4 IO Test Mouse lgG1 PE A07796 100 2mL 5 Ficoll Paque Phamacia 17 0840 03 2 1 100 L 5x 103 mm3 10 x 10 mm PBS 3 x 109mm
13. L Cross G D White M D 1997 Revised 9uidelines for performing CD4 T cell determinations in persons infected with human immunodeficlency virus HIV 1997 Morbidity and Mortality Weekly Report RR 2 46 1 29 8 Bray R A Gebel H M Applications of flow cytometry to transplantation of solid organs 1990 Labmedica Feb March 28 30 9 Velardi A Terenzi A Cucciaioni S Millo R Grossi C E Grignani F Martelli M F Imbalance within the peripheral blood T helper CD4 and T suppressor CD8 cell populations in the reconstitution phase after human bone marrow transplantation 1988 Blood 5 71 1196 1200 10 Bernard A Brottier P Georget E Lepage V Boumsell L Joint report of the first international workshop on human leucocyte differentiation antigens by the investigators of the participating laboratories 1984 Leucocyte Typing Bernard A et al Springer Verlag 9 135 Kung P Goldstein G et al 1979 Sclence 206 347 349 135 0063 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460 135 0063 5 7 TOC A07757 1210
14. 196 2 3 4 5 6 7 8 2 8 C 12
15. 2012 10 1 20200EZY00111000 A07757 oe COULTER I T IO PE CD8 IOT8 1 3 4 I IO PE CD8 IOT8 PE IgG CD8 76kD 32 34kD CD8 T CD8 MHC 1
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