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Contents
1. 2 PCR cDNA 22. 0 2 1 M 0 05 0 3 M SNPs 2 PCR
3. 20 30mer GC 40 60 200bp 150bp PCR PCR ABI PRISMP7700 LightCycler LineGene PCR 2 DNA cDNAI 1st strand cDNA cDNA dT
4. 60 15 5 data collection 30 PCRH 2 60 95C 60 95C 15 x40 60C 60 data collection 2 Roche LightCycler 1 PCR 0 3uM 0 2 0 4uM 20pL 5 6 uL Realtime PCR Master Mix 10 AL 1 10 M 0 6 wL 2 10w M 0 6 wL 1 5 acceptor 10 M 0 8 L 2 3 donon 10 uM 0 4 MWL 2 pL 20 wwL
5. 60 15 5 PCR 3 60 72C 95C 60 95C 15 x40 60C 15 72C 30 data collection 4 1 RT PCR 1 cDNA DNA DNA POCHR RNA 1 RT PCR TagMan 1 RT PCR PCR
6. 530 8230 2 8 TEL 06 6348 3786 FAX 06 6348 3833 E mail order_lifescience toyobo jp 141 8633 10 2 TEL 03 6422 4819 FAX 03 6422 4951 E mail order_lifescience toyobo jp TEL 06 6348 3888 FAX 06 6348 3833 9 00 12 00 13 00 17 00 E mail tech_osaka toyobo jp URL http www toyobo co jp bio
7. ReverTra Ace Code TRT 101 2 PCR RNase Inhibitor Code SIN 201 0 5U L ReverTra Ace 0 01 1U L 1U L ReverTra Ace ReverTra Ace RNase Inhibitor 5U L ReverTra Ace RT RT DNA DNA RT RT ReverTra Ace
8. Taq PCR 10 1 LightCycler PCH 2 QPK 101 QPK 101X5 50L 50L x 200 x 1000 20 C Realime PCR 4 Master Mix 2 UN MUSE Realtime PCR Master Mix dNTP MgCls Tagq DNA Taqdqd PCR 2 DNA
9. PCR cDNA ReverTra Ace qPCR RT Kit Code FSQ 101 209 1st strand cDNA ReverTra Ace w Code FSK 101 10 cDNA PCR DNA PCR DNA DNA 1 lt 10ng 4 1 ABI PRISMY7700 TaqMan 1 PCR 0 4uM 0 2wM 50L 14 AL Realtime PCR Master Mix 25 L 1 10guM 2 AL 2 10M 2 AL TagMan 5M 2 AL 5 AL 50 AL Realtime PCR Master Mix
10. Tm 30 5 5 PCR 50 C 15 75C 30 95C 30 95C 5 x40 50 15 data collection 75C 30 3 BioFlux LineGene TaqMan 1 PCR 0 4uM 0 2wM 10L 1 8 L Realtime PCR Master Mix 5 uuL 1 10 M 0 4 wL 2 10w M 0 4 wL TagMan 5M 0 4 AL 2 pL 10 AL Realtime PCR Master Mix 1 2
11. 3 PCR PCR 1 2 3 42C 20 5 PCR 1 2 3 Tm ABI PRISM 7700 42 C 20 95 C 5 95 C 15 x40 60 C 60 data collection 5 1 1
12. PCR cDNA ReverTra Ace qPCR RT Master Mix 200 FSQ 301 with gDNA Remover 10 000U x 1 TRT 101 ReverTra Ace9 10 000Ux 1 x 5 RT 101X5 10 000Ux 1 x 10 RT 101X10 DNA RNase 2 500U x 1 SIN 201 RNase Inhibitor recombinant 2 500Ux 1 x5 SIN 201X5 PCR SYBR Green 1mL x 5 QPK 201 SYBR Green Realtime PCR Master Mix 1mLx 5 x5 QPK 201X5 PCR BR Green yt 0 ImLx5 QPK212 SYBR Green Realtime PCR Master Mix Plus 1mLX5 X5 1mLx1 QPS 101T PCHR 1 67mLx3 QPS 101 THUNDERBIRD Probe qPCR Mix 1 67mL x 3 x5 QPS 101X5 SYBR Green PCR 1 0 Nd THUNDERBIRD SYBR qPCR Mix 1 67mLx 3 x5 QPS 201X5 500Lx5 QRT 101 RNA direct Realtime PCR Master Mix S00 EX RS RDNS 1 step PCH SYBR Green 500Lx5 QRT 201 RNA directy SYBR Green 500Lx5 x5 QRT 201X5 Realtime PCR Master Mix
13. cDNA PCR PCR 6 ReverTra Ace cDNA ReverTra Ace w 100 ESKOI PCR cDNA ReverTra Ace qPCR RT Kit 200 FSG 101 PCR cDNA ReverTra Ace qPCR RT Master Mix 200 Re DNA
14. 2 FRET 1 LC Red640 2 3 FITC Realtime PCR Master Mix 1 02 1 2 w 2 PCR Tm data
15. 1 20 10 2 4 QPK 101 1 20C 4 C 3 1
16. 1 2 0 2 1 M 0 05 0 3 M SNPs 2 PCR
17. 3U L 0 3U L RT RNase Inhibitor 5U L 32 AL ReverTra Ace 100U L 1 AL 33 AL PCR TaqMan 9 AL Realtime PCR Master Mix 25 uL 1 10w M 2 MWL 2 10 M 2 AL TagMan 5wM 2 AL RT ReverTra Ace 3U L 5 AL 5 pL 50 AL Realtime PCR Master Mix 1 26 1
18. California 94404 USA LightCycler Idaho Technology Inc Roche Molecular Systems Inc TaqMan Roche Molecular Systems Inc ABI PRISM Applera Corporation 1 1 DNA 2x TaqMan PCR PCR 2 LightCycler BSA ABI PRISMP7700
19. ldeas amp Chemistry Realtime PCR Master Mix Code No QPK 101 QPK 101X5 TOYOBO CO LTD Life Science Department OSAKA JAPAN A3313K 1 2 3 3 HOE RE DE CE BG I Rs 4 5 1 ABI PRISM 7700 TaqMan 5 2 Roche LightCycler 6 3 BioFlux LineGene TagMan 7 4 1 RT PCR 8 5 10 6 11 NOTICE TO PURCHASER LIMITED LICENSE A license to perform the patented 5 Nuclease Process for research is obtained by the purchase of i both Authori
20. zed 5 Nuclease Core Kit and Licensed Probe ii a Licensed 5 Nuclease Kit or iii license rights from Applied Biosystems This product is an Authorized 5 Nuclease Core Kit Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US 5 079 352 5 789 224 5 618 711 6 127 155 5 677 152 5 773 258 5 407 800 5 322 770 5 310 652 5 210 015 5 487 972 and claims outside the US corresponding to US Patent No 4 889 818 The purchase of this product includes a limited non transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser s own internal research Separate purchase of a Licensed Probe would convey rights under the applicable claims of US Patents Nos 5 538 848 5 723 591 5 876 930 6 030 787 6 258 569 5 804 375 claims 1 12 only and 6 214 979 and corresponding claims outside the United States No right under any other patent claim and no right to perform commercial services of any kind including without limitation reporting the results of purchaser s activities for a fee or other commercial consideration is conveyed expressly by implication or by estoppel This product is for research use only Diagnostic uses under Roche patents require a separate license from Roche Further information on purchasing licenses may be obtained from the Director of Licensing Applied Biosystems 850 LincoIn Centre Drive Foster City
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