IO テスト PE 標識抗体 CD19 IOB4a
Contents
1. 6 7 Ficoll Paque 8 9 10 112. IO 95 20 40 CD14 Raji 1 0 5 1 Raji Burkittl lyphoma PBS 4 3 10 3 5 5 1
3. PE Log 2 2 EPICS XL vs PE LumphocutesS Leukocutes 121 6 18 RD1 i100 1000 ON CYTO TROL 248 Fc
4. 1 3 1 PBS PBS 3 1 500mL 0 2 BSA 0 5 PBS VersaLyse PBS 1mL A07800 I IM3515 12 5L 0 19 PBS rsaLyse PBS 1mL Test 3 A07800 I IM3515 67 5L 0 5 PBS 3 A09777 IM3648 VersaLyse IM140
5. IOTest C CD14 PE A07738 IM1201 K T B NK
6. 0 19
7. 001 3 5 1 Immunotech a Beckman Coulter Company 130 avenue de Lattre de Tassigny B P 177 13276 Marseille Cedex 9 France
8. 4 LH700 5 PBS 5 10 x 10 mm3 1 12mm x 75mm 2 100 L 3 20uxL IOTest Mouse Il9G1 PE 20uL 4 15 5 6 PBS 2mL 7 400 x g 3 8 7 8 9 0 5 1mL 0 5 PBS
9. 10 24 3 Ficoll Paque 1 3 4mL PBS 2 Ficoll Paque 3 2 8 C 400xg 30 4 Ficoll Paque 5 PBS 2 8C 400xg 8 6 PBS 7 2 8 C 400 x g 4 4mL 1 8 PBS 9 2 8 400 x g 3 10 PBS
10. IO PE CD19 IOB4a 2 8C 12 IO PE CD19 IOB4a A07769 100 2mL 1 Carter R H Doody G M Bolen J B Fearon DT Tedder F lsaacs C M Pen Doody G M Dempsey P W Fearon D T Activation of B lymphocytes integrating signals from CD19 CD22 and FcyRIIb1 1996 Cur Opin Immunol 8 378 382 lt Bradbuy L E Kansas G S Levy S Evans R L Tedder LF 105 0001 3 5 1 The CD19 CD21 signal transducing complex of human lymphocytes includes the target of antiproliferative anti Leu 13 molecules 1992 J Immunol 9 149 2841 28 lgM induced tyrosine phosphorylation of CD19 require domain that mediates association with com antigen receptor complex 1997 J Immu Tedder T F Zhou L J Engel P T ignal transduction complex of B lymphocy mmunol Today 9 15 437 442 CD19 a member of the i transfected cells to exa Leucocyte Typing IV W ntiation Antigens W Knapp et al Eds Oxford
11. Ti CD1 J4 119 IgG1 SKLY18 PE Phycoerythrin 486 560 590nm 1 IO 2 EDTA 6 4 CO 5
12. 2006 3 2004 10 202EZY00111000 A07769 IO PE CD19 IOB4a 1 2 3 4 IO PE CD19 IOB4a PE lgG 20L 1 CD19 95kD CD19 B
13. mm3 5 x 106 mL 11 90 1 5x105 L 2 0 Fict IOTest Mouse 20L C 30 gt 2 8C 400 xg 5 0 5 1mL 0 1 0 5 BBS 24 FS 90 SS L
14. 0 OptiLyse B A11895 A11894 OptiLyse C A07799 I IM3514 IOTest 3 4 IOTest Mouse IgG1 PE A07796 I IM0670 100 2mL 5 Ficol Paque Phamacia 17 0840 03 2 1 100 L 5x 10 mm 10x10 mm PBS 3x 10 mm 1 25C 500 x g 5 2 3
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