（哺乳類細胞、昆虫細胞、バキュロウィルス、大腸菌）PDF
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1. 14 3 20 PEG 2 7 500g 1 15 8 9 1 5 1 10 Pellet 5 10 pellet 11 1 0ml 12 80C 100 12. 10 5 000g 5 12 2 6 3 5 lt 10 20 w v PEG MW 8 000 1 2 3 4 5 6 Option debris 1 000 g 10 1 20 PEG 30 500g 30 1 Pellet 13
3. Q3 A3 1 90 CEM HOS CHO HUT78 COS7 Jurkat E coli DH5c BL21 P3 NS 1 1 Ag4 1 HEK293 Raji Hela Sf9 HepG2 T cell hybridoma HL60 WEHI 3 Q4 1 2 A4 1 2 2
4. 4 2 27 0 5 ml 2 00 1 0 ml 2 15 37C 0 5 ml 1 40 1 0 ml 1 55 2 20 2 30 2 00 2 10 6 2 1 1 2 3 4 LB 0D600 0 6 1 0 5 2 9 80 C 1 1
5. 2 4C 4C 9 1 2 1 3 1 pH 7 0 8 5 2 20EU ml 4 80 Jurkat CHO 4 3 5 1 2 3 4 6 6 1 1
6. 1 80C 1ml Q2 5x105 5X106 cells ml A2 1
7. 8 2 8 3 1 2 3 4
8. 2 3 2 8 9 50 10 2 11 100 100 1 2 3 4
9. 103 0001 14 10 Liens 4 TEL 03 6264 9961 FAX 03 6264 9962 E mail info jujfield co jp ct gt
10. Q5 1 A5 1 1 1 Q6 A6 8 8 1 MSDS
11. 4 1 PBS 2 PBS Trypsin EDTA PBS 5 3 pipetting 4 5 200 300 g 5 6 5X10 5x108 cells ml 7 6 0 5ml 1 0ml 8 10 9 80C 3 10 24 196 1 6well 2 ml 2x105 1X106 cells 10cm 10 ml 1x106 5x106 cells 15cm 20 ml 2x106 1X107 cells 1
12. 27C 16 2 2 3 7 Q1 A1 Trypsin Trypsin 1 2 mM EDTA PBS
13. 2 1 3 4 2 1 2 10 6
14. 3 5 7 4 2 1 2 5 1 6 2 7 COz 2 5 DMSO HL60 6 80C 2 7 1 2
15. BLB2014 12 x Code No 1 100 ml x 1 BLB 1 20 mlx 4 BLB 1S 2 100 ml x 1 BLB 2 20 mlx 4 BLB 2S 1 DMSO 10
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