T11-RD1/B4-FITC
Contents
1. No Wash No Wash Q PREP TQ Prep Q PREP TQ Prep ECT T B2. PBS 20 25 C 6 a gt 10 x 10 mm 10 20 x 10 2 20 30 x 10 3 dE 30 40 x 10 5 40 60 x 10 6 60 100 x 10 10 100 200 x 10 20 b 3 x 10 mm 276 1 25C 500 xg 2 LET LH 700 10 x 10 mm 1 ULARLY 3 4
3. Min Max Mean 1SD T11 67 87 78 4 4 9 B4 5 15 9 3 2 2 T11 mm3 1020 2041 1 479 2 270 1 B4 mm 81 271 177 3 48 2 T11 B4 CD2 CD19 l CD2 CD19 1 CD2 CD19 3 31 T11 B4 96CV CD2
4. PMT 1 2 3 EDTA 6 4 90 5
5. 3 FITC Log U Log RD1 PE Log 2 RD1 PE FITC CD2 Quadrant 1 T11 B4 0 CD19 Quadrant 4 T11 B4 CD2 CD19 Quadrant 2 T11 B4 1 Q PREP A R51 d 1 166 1688 18 FITC 2 168 1868 CD2 CD19 Flow Count LH 700 mm mm3 x 96 x 104
6. 6 7 8 9 TQ Prep Multi Q Prep 11 CD2 HRI d TORAH T NK
7. CYTO TROL 6604248 Fc Mo2 RD1 KC56 FITC CD14 CD45 Fc MSlgG1 RD1 MSlgG1 FITC 1 29 Quadrant1 2 4 29
8. T B T B CD4 T CD8 T CD4 CD8 AIDS HIV T U B T CD4 21 95 n 30 T11 B4 EPICS PROFILE
9. T NEF T Harvard Medical School Dr S F Schlossman Coulter Immunology T B 3 6 3 T B T
10. 9 25 2 PBS PBS 6603369 500mL x15 1 500mL pH 7 2 0 2 3 MslgG1 RD1 MslgG1 FITC 6603769 50 0 5mL EDTA 1 100L 1 1 2mL 3 10 x 10 mm3 10x 10 8 mm 3 x 103 mm Q PREP
11. 6603801 2009 11 2007 2 20100EZY00149000 877449 T B T11 RD1 B4 FITC 2 FITC Fluorescein isothyocyanate RDI Phycoerythrin PE 1 wo gt T11 RD1 02 1240g T Ah B4 FITC 0 2 3 0 4 g 7 AF T11 RD1 CD2 50kD CD2
12. 5 TQ Prep Multi Q Prep 6 25 35 EPICS 7 2 2 2 8 C 24 2 1 2 100L 3 10 L MSlgG1 RD1 MSlgG1 FITC 10L 4 45 5 PBS 2 3mL 400 450 X g 5 6 7
13. PBS 25 1mL 30 2 8 250L MA LET 9 PBS 2mL 10 400 450 X g 5 11 12 9 11 13 PBS 500L 14 EPICS 1 2 Q PREP EPICS FS Q PREP IK 0 5mL PARE E DISK EM
14. 2 20 25 C 3 T11 RD1 B4 FITC 6603801 50 0 5mL 1 Bernard A Boumsell L Dausset J Milstein C and Schlossman SF eds 1984 Leukocyte Typing New York Springer Verlag p 26 28 41 42 196 201 215 216 2 McMichael AJ ed 1987 Leukocyte Typing III Oxford Oxford University Press p 38 40 42 106 107 116 199 302 306 308 315 475 3 Reinherz EL Haynes BF Nadler LM and Bernstein ID 1986 Leukocyte Typing I New York Springer Verlag Vol 1 p 6 16 364 365 4 Reinherz El Haynes BF Nadler LM and Bernstein ID 1986 Leukocyte Typing II New York Springer Verlag VoL 2 p 8 15 25 37 5 Reinherz EL and Schlossman SF 1980 The differentiation and function of human T Iymphcctes Cell 821 827 6 Aiuta F Cerottini J C Coombs RRA Cooper M Dickler HB Froland S F
15. B T Ig T B E Smlg E T T Smlg B lg Fc lg E T B T B
16. B B M CD21 CD22 B B CD22 pre pre B CD22 Pan T Pan B T B Pan T Pan B CD2 CD19 T11 B4 KOC AMO T B
17. y 0 95x 0 7 r 0 97 n 56 1 4 6 2 3 4 5 6 1 28 C
18. E pan T 95 TN TOA T NK CD2 T CD2 Halt AMMO B B4 FTIC CD19 95kD CD19 pan B B B B 909 59 CD19 B T T11 SFCI3Pt2H9 CD2 T BALB c NS 1
19. TdT HLA DR Smlg BET B T B T pan T CD7 CD2 CD5 CD3 CD3 CD4 CD8 B pan B CD19 B pre pre B CD20 pre B CD19 CD20 B B
20. CD2 BIERI RA T CD3 12 1 10L 1 TQ Prep Multi Q Prep 7546999 300 TQ Prep Multi QPrep 3 A B C 2 1 6603152 300 1mL PBS 24mL 25 x
21. PB44 CD19 PB11 T11 9 B4 n Mean 1SD CV Mean 1SD CV 1 31 17 9 0 8 4 2 81 9 1 0 1 2 2 31 64 2 0 6 1 0 36 8 0 9 2 5 3 31 92 3 0 5 0 6 75 0 5 6 7 2 3 1 3 11 T11 B4 CV T11 B4 n Mean 1SD 96CV Mean 1SD CV 1 EPICS PROFILE 11 732 08 11 131 07 53 2 EPICS PROFILEI 11 750 07 09 134406 45 3 EPICS 753 11 732409 12 127404 31 T11 RD1 B4 FITC CD2 y 1 00x 1 3 r 0 93 CD19
22. RD1 FITC 4 FS 90 SS FITC RD1 CYTO COMP CYTO COMP CELL T8 FITC T8 RD1
23. 5 100 1 Q PREP TQ Prep Multi Q Prep Coulter Immunology 3 A B C TQ Prep Muiti Q PREP 1 12mm x 75mm 2 100L 3 MSIgG1 RD1 MSIgG1 FITC 10L 4 10 12
24. AG4 B4 89B B4 B BALB cJ NS 1 AG4 lg lgG1H zL SRCTT1 B4 T11 B4 T11 B4 1 6 T11 RD1 RD1 Phycoerythrin PE RD1 05 1 5 486 580nm 568 590nm B4 FITC FITC Fluorescein Isothyocyanate FITC 5 10 468 509nm 504 541nm 1 0 5mL BSA 0 2 0 01M NaCl 0 15M NaN3 0 1 E T B 2 T CD2 CD19 488nm
25. Clin Immunol Immunopathol 52 19 27 135 0063 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460 135 0063 5 7 TOC 576 6603801_0911 6 6
26. inical Medicine McMichael AJ and Fabre JW eds New York NY Academic Press p 39 70 Schmidt Re 1989 Monoclonal antibodies for diagnosis of immunodeficiencies Blut 59 200 206 deMartini RM and Parker JW 1989 Immunobgic alterations in human immunodeficiency virus infection A review J Clin Lab Anal 3 56 70 Fauci AS 1988 The human deficiency virus Infectivity and mechanism of pathogenesis Science 239 617 622 10 13 14 15 16 17 Taylor MGJ Fahey JL Detets R and Giorgi JVI1989 CD4Percentage CD4number and CD41CD8ratio in HIV infection How to choose and how to use JAIDS 2 114 124 18 Pedrazzini A Freedman AS Andersen J Heflin L Anderson K Takvorian T Canellos GP Whitman J Coral F Ritz J and Nadler LM 1989 Anti B cell monoclonal antibody purged autologous bone marrow transplantation for B cell non Hodgkin s lymphomal Phenotypic reconstitution and B cell function Blood 74 2203 2211 19 Ramos EL Turka LA Leggat JE Wood IG Milford EL and Carpenter CB 1989 Decrease in phenotypically defined T helper inducer cells cr4 4B4 and increase in T suppressor effecter cells T8 2H4 in stable renal allograft recipients Transplantation 47 465 471 20 Loken MR Brosnan JM Bach BA and Ault KA I 990 Establishing optimal lymphocyte gates for immunophenotyping by flow cytometry Cytometry 11 453 459 21 Koepke JA and Landay AL 1989 Precision and accuracy of absolute lymphocyte counts
27. udenberg HH Greaves MF Grey HM Kunkel HG Natvig J Preud homme J L Rabellino E Ritts RE Rowe DS Seligmann M Siegal FP Stjernsward J Terry WD and Wybran J 1975 Identiffication enumeration and isolation of B and T Iymphocytes from human peripheral blood international Union of Immunobgical Societies IUIS Report July 1974 Clin Immunol and Immunopathol 3 584 597 7 Nadler LM Anderson KC Marti G Bates M Park E Daley JF and Schlossman SF 1983 B4 a human B Iymphocyte associated antigen expressed on normal mitogen activated and malignant B lymphocytes J Immunol 131 244 250 8 Benjamini E and Leskowitz S 1991 Disorders of the Immune Response In Immunology A Short Course Second Edition New York Wiley Liss p211 244 9 Reinherz EL 0 Bhen C Rosenthal P and Schlossman SF 1980 The cellular basis for viral induced immunodeficiency Analysis by monoclonal antibodies J Immunol 125 1269 1274 Felsenstein D Camey WP lacoviello VR and Hirsch MS 1985 Phenotypic properties of atypical Iymphocytes in cytomegabvirus induced mononucleosis J Infect Dis 152 198 203 11 Rinaldo CR Ho M Hamoudi WH Gui X and DeBiasio RL 1983 Lymphocyte subsets and natural killer cell responses during cytomegalovirus mononucleosis Infect Immnun 40 472 477 Goldstein G Litter J and Mittler R 1982 Immunoregulatory changes in human disease detected by monoclonal antibodies to T Iymphocytes In Monoclonal Antibodies in Cl
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T11 RD1/B4 FITC