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沈降速度法
Contents
1. A native
2. C C
3. C 9 0 1 Main Peak Monomer 45 5 1 Chain 1 4 Dimier 30 6 5 3 1 4 Q Q 6 lt N Q O 0 4 HL Half Molecule 0 8 er Free Light 1 ntamer Xamer Pe He Il2 1l4 16 18 20 22 24 Sedimentation Coefficient Svedbergs B Abs ee A
4. A B 4 IgG 0 19
5. Da g mL mg mL A A ProteomeLab XL A XL
6. 0 2 4 6 8 10121I416182022 24 26 28 30 6 8 10 12 I4 16 18 20 22 24 26 28 30 Sedimentation Coefficient Svedbersgs C Source J 5 Philo Ph D Alliance Protein Laboratories Molecular Conformation ME TE I ris EDTA 27 0S 1 8 mM 33 5 5
7. Nano Particle Size DistriDution od 6 Cumulative Fraction Cumulative Fraction _20 30 40 50 60 _20 30 40 50 60 S20 w S20 w SOU Ce Jeffrey C Hansen Ph D Dept of Biochemistry University of exas Health Science Center SanAntonio Texas USA ME 3 SO0 50 100 nm 0 50 100 nm 15 000 rpm 0 0 5 0 50 00 50 20o 00 00 00 400 S S S 20 50 100nm 20nm 50nm 100nm 0 5 10 15 20 25 0 50 100 150 200 100 2
8. Concentration Meniscus Rd Bottom A Abs B G In Abs hl C T Dm 400 L 150 L
9. Dy aAcquire Zoom Palette Control ProteomeLab XL Protein Heterogeneity 4 4 S A B
10. 60 000rpm 90 000g An 60Ti 50 1 000 rpm 10 0C 40C 1 CO 050 190nm 800nm 660nm 10 10 Da 5 g mL 1 2 mg mL ProteomeLab XL 5 g mL 4 5mg mL a 7 3 8 K a 1 190nm 10mM NaPO4 0 15 MKF 9340 W X673 D X1 207 H mm PC pH7 5 g mL LHRH 10 mM MOPS 0 15 M NaCl pH7 5 200V 50 60Hz 30A m CI p 4mg mL BSA 58 uM ProteomLab XL A XL 1 mm 3mm 56 et XL A ee A CE a
11. 3 0 196
12. opH 4 8 rpm A 2 ProteomeLab XL A ProteomeLab XL X XL A 180 800nm
13. 3 1nm A 381nm B Inhibitor 321nm Compound does not bind to A Compound binds to B 7 MW 59 770 Da 06 0 04 Absorbance 321nm CN C 3 03 64 645 65 655 66 RadiuS cm RadiuS cm SOU Ce Michelle Arkin Ph D Sunesis PharmaceuticalS Inc ProteomeLabD XXL A XXL
14. An 60Ti 4 Epon ChacoalFilled 3 A An 60Ti 4 Epon Charcoal Filled 3 Epon CharcoaFiled 3 Ne Epon Charcoal Filled 7 80 4 Epon Aluminum Filled 7 Epon Charcoal Filled 7 Epon Charcoal Filled 7 0 6 IE NM 6 XL Te A An 60Ti 4 Epon Charcoal Filled 4 Epon Charcoal Filled 4 8 Epon Charcoal Filled 8 XL An 6OTi 4 _ ei B An 5OTI 8 a 8 Epon Charcoal Filled 4 Epon Charcoal Filled 8 ProteomLab XL A XL An 60Ti 4 An 50Ti 8 rpm 60 000 50 000 xg SO 01 00 3 4 8 4 Te NE 6 An 50Ti 8 Beckman Coulter Beckman Coulter Beckman Coulter Inc Epon Shell Ch
15. XL 1282 mm 3mm 6 3 pH An 50Ti 8 8 24 8 1
16. with density or molecular weight by D analytical utracentrifugation Nat Commun 2 335 doi NS SUOMI SS SON ND tumor necrosis factor sequence homolog log absorbance 02 00 02 04 0 r2 r 2 2 sSource J 6 Philo Ph D Alliance Protein Laboratories ME
17. 00 300 400 S S 93 Carney R P et al Determination of nanoparticle size distriDution together
18. B COULTER Brilliance at every 777777 4
19. emical A 135 0063 3 5 7 TOC 0120 566 730 03 6745 4704 03 5530 2460 ER bckkcas beckman com http www beckmancoulter co jD B01002 2015 04 1000 L
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