広島県で分離された腸管出血性大腸菌 O157 の分子疫学的解析
Contents
1. 25 MLVA 1 2014 4 2014 355 117 119 2 Escherichia colz STEC 2009 p 281 296 3 0157 73 4 5 6 F No 22 2014 2013 32 40 442. IS printing system 1S Multi locus variable number tandem repeat analysis MLVA 3 4 2013 EHEC 0157 PFGE IS MLVA 1 2013 1 12 EHEC O157 23 1 8 3 A B C 2 1 PFGE 5 Xba 1 TaKaRa BIO RAD CHEF MAPPER BioRad 6V cm 216 63 8 14 18 PFGE BioNumerics Ver6 6 Applied Maths Dice UPGMA
3. PFGE Type No TN No 22 2014 1 EHEC O157 13 01 2013 1 12 A 63 O157 H7 VT12 13 02 03 04 13 02 2013 1 17 A 41 0157 H7 VT12 13 01 13 03 2013 1 16 A 11 O157 H7 VT12 13 01 13 04 2013 1 16 A 14 O157 H7 VT12 13 01 13 05 2013 3 21 B 17 0157 H7 VT2 13 06 2013 4 16 A 8 0157 H7 VT12 13 07 2013 6 13 A 78 O157 H7 VT2 13 08 12 13 08 2013 6 18 A 80 0157 H7 VT2 13 07 12 13 09 2013 6 27 B 41 O157 H7 VT12 13 10 2013 6 28 A 13 0157 H7 VT2 13 11 13 14 13 11 2013 6 29 A 7 O157 H7 VT2 13 10 13 12 2013 6 29 A 91 0157 H7 VT2 13 07 08 13 13 2013 7 1 A 45 0157 H7 VT2 13 10 13 14 2013 7 1 A 27 0157 H7 VT2 13 12 13 15 2013 7 4 A 11 O157 H7 VT12 13 16 2013 7 5 A 16 O157 H7 VT2 13 10 13 19 2013 8 1 A 38 O157 H7 VT12 13 21 2013 8 7 A 11 O157 H7 VT12 13 22 2013 8 13 A 2 0157 H7 VT12 13 32 2013 8 31 A 19 0157 H7 VT2 13 34 2013 10 3 A 3 0157 H7 VT12 13 35 201
4. No 22 p9 14 2014 0157 Molecular Epidemiological Analysis of Enterohemorrhagric Escherichia coli O157 detected in Hiroshima Prefecture HIROKO YAMADA KAZUMI IMAr KANAkO MAsUDA and SHINICHI TAKAO Received October 16 2014 2013 0157 PFGE IS printing system IS Multilocus variable number tandem repeat analysis MLVA PFGE IS MLVA 1 3 MLVA IS PFGE Key words 0157 IS printing S
5. 2008 p 101 128 O157 IS printing system 2013 34 5 127 128
6. 13 01 04 4 PFGE TN i12 13 07 08 10 14 16 8 PFGE TN g501 PFGE 13 06 15 PFGE TN i49 13 21 22 PFGE TN i399 13 37 13 01 04 PFGE TN i12 PFGE 13 09 06 15 PFGE TN i49 1 97 6 13 35 2 920 2 IS EHEC O157 23 IS 11 PFGE 3 PFGE IS IS PFGE IS 341057 310652 13 07 08 10 14 16 1st 3 MLVA 9 1 single locus variant SLV
7. 0157 3 2009 44 45 48 15 2004 p 10 21 Izumiya H Pei Y Terajima J Ohnishi M Hayashi T Iyoda S Watanabe H New system for multilocus variable number tandem repeat analysis of the enterohemorrhagic Escherichia coli strains belonging to three major serogrOuDS O157 026 and Olll Microbiol Immunol 2010 54 569 577 7 Tenover FC Arbeit RD Goering RV Mickelsen 8 9 FF PA Murray BE Persing DH Swaminathan B Interpreting chromosomal DNA restriction patterns produced by pulsed field gel electrophoresis criteria for bacterial strain typing J Clin Microbiol 1995 33 9 2233 2239 19
8. 8 2 3 PFGE 13 01 04 PFGE TN i12 4 0157 3 1 13 02 0157 10 1 13 04 1 4 905 T 3 EHEC 0157 PFGE IS MLVA IS MLVA repeat numbers 0 NN na O157 O157 O157 O157 O157 O157 O157 O157 0157 ee 3 9 25 34 10 17 19 37 36 13 01 12 9 4 11 27 7 4 6 9 WV 13 02 13 9 4 11 27 4 6 9 13 03 77 611557 12 9 4 1 27 7 4 6 9 13 04 12 9 4 11 28 7 4 6 9 13 37 i440 717557 631557 12 9 4 11 28 6 4 6 9 13 32 i393 305457 611242 14 9 5 9 33 4 7 7 6 MI 13 19 i206 717577 611657 11 11 8 12 8 7 6 5 3 13 21 10 13 8 12 29 8 6 5 3 WW 13 22 1399 17555 611657 1j0 13 8 12 29 8 6 5 3 13 06 10 12 3 11 29 7 7 8 11 I 028 10 12 3 11 32 7 7 8 11 13 09 i197 717575 211657 10 12 3 11 27 7 7 8 11 13 35 i603 717555 211657 10 12 3 11 30 7 7 8 11 13 07 18 9 3 9 16 5 8 8 2 I 13 08 18 9 3 9 16 5 8 8 2 13 10 18 9 3 9 16 5 8 8 2 13 11 A 18 9 3 9 16 5 8 8 2 1312 8501 341057 310652 6 5 0 6 6 6 13 13 19 9 3 9
9. 13 01 04 13 07 08 10 14 16 2 PFGE IS MLVA 1 1 PFGE 0 2 3 4 6 7 MLVA 0 1 SLV 2 13 37 13 01 04 MLVA 2 PFGE 4 9 13 06 09 15 35 MLVA 1 PFGE IS 2 13 06 15 3
10. 12 IS Izumiya 6 2 9 QIAGEN Multiplex PCR Kit QIAGEN 2 PCR 3500 Genetic Analyzer Applied biosystems Gene Mapper Software 5 Applied biosystems Fragment size marker GeneScan 600 LIZ Size Standard v2 0 Applied biosystems No 22 2014 Big Dye Terminator v3 1 Cycle Sequencing Kit Applied biosystems 2 BioNumerics Ver6 6 Appled Maths categorical coefficient UPGMA 1 PFGE EHEC O157 23 PFGE 11 1 3
11. 16 5 8 8 2 13 14 18 9 3 9 16 5 8 8 2 13 16 18 9 3 9 16 5 8 8 2 13 05 i48 105457 311252 16 11 4 9 37 6 6 7 5 V 13 34 i348 717557 611657 10 14 3 11 26 7 7 8 10 I single locus variant No 22 2014 ll a a PFGE PFGE 8 RS EE RR EE kbp Type No UT so IT 13 02 _ i 13 03 12 HN 13 04 13 37 i440 EE Ii 1 13 32 i393 13 19 i206 13 21 Hi 13 22 399 Nil 1306 gg l 13 15 13 09 i197 13 35 i603 M I NI iso7 Ni 1308 HH Ni 13 10 13 11 13 12 g501 ll 13 13 fi 1 1 13 14 ll 13 16 fi 1305 iag HT 13 34 is4e 1 EHEC 0157 PFGE MLVA repeat numbers RB E WW W 5 5 5 8 5 5 8 8 5 10 12 3 11 29 7 7 8 11 13 06 10 12 3 11 27 7 7 8 11 13 09 10 12 3 11 32 7 7 8 11 13 15 I 10 12 3 11 30 4 8 11 13 35 10 14 3 1 26 7 7 8 10 13 34 I 18 9 3 9 16 5 8 8 2 2 13 07 18 9 3 9 16 5 8 8 2 13 08 18 9 3 9 16 5 8 8 2 13 10 18 9 3 9 16 5 8 8 2 13 11 18 9 3 9 16 5 8 8 2 13 12 18 9 3 9 16 5 8 8 13 14 18 9 3 9 16 5 8 8 2 13 16 19 9 3 9 16 5 8 8 2 13 13 12 9 4 11 28 7 4 6 9 13 04 12 9 4 11 28 6 4 6 9 13 37 12
12. 3 10 8 A 21 O157 H7 VT12 13 37 2013 10 29 C 32 0157 H7 VT12 2 MLVA Locus Locus Sequence 5 3 7 Dye A Offset Mixl 0157 3 F GGCGGTAAGGACAACGGGGTGTTTGAATTG 0 04 NED 6 323 R GAACAACCTAAAACCCGCCTCGCCATCG 0 04 O1579 F GCGCTGGTTTAGCCATCGCCTTCTTCC 0 04 VIC 6 465 R GTGTCAGGTGAGCTACAGCCCGCTTACGCTC 0 08 O157 25 F GCCGGAGGAGGGTGATGAGCGGTTATATTTAGTG 0 04 PET 6 110 R GCGCTGAAAAGACATTCTCTGTTTGGTTTACAC 0 04 O157 34 F TGTTACCAACGCGAAGCTAACAAG 004 6FAM 18 141 R AGGCATTAATAGCAGATGTTC 0 08 Mix2 O157 10 F CAAACTTTACTGTTCATTTCTACAGTCTC 0 04 VIC 6 350 R AGGTATTATAAGGGGCTTAAAGATAAAAG 0 08 O157 17 F GCAGTTGCTCGGTTTTAACATTGCAGTGATGA 0 04 VIC 6 121 R _ AGAAATGGTTTACATGAGTTTGACGATGGCGATC 0 04 O0157 19 F GCAGTGATCATTATTAGCACCGCTTTCTGGATGTTC 0 04 NED 6 273 R CGGGCAGGGAATAAGGCCACCTGTTAAGC 0 04 O157 37 F AATCAGAGCGGCAGGAAAAAGAAGA 0 04 PET 6 84 R GGGCTTCTGTCTTTTCAGACCTG 0 04 O157 36 F GGCGTCCTTCATCGGCCTGTCCGTTAAAC 0 04 NED 7 102 R GCCGCTGAAAGCCCACACCATGC 0 04 2 IS 3 MLVA IS printing system 2 lst set 2nd set 1 0 3 1 2 4 lst set 2nd set
13. 5 13 09 15 13 21 22 PFGE IS MLVA 3 EHEC 0157 MLVA PFGE 1S PFGE MLVA MLVA 1 2 PFGE I
14. 9 4 11 27 7 4 6 9 13 011V 12 9 4 11 27 7 4 6 9 13 03 13 9 4 11 27 7 4 6 9 13 02 16 11 4 9 37 6 6 7 5 13 05 V 14 9 5 9 33 4 YA 7 6 13 32 VI 10 13 8 12 29 8 6 5 3 13 21 10 13 8 12 29 8 6 5 3 13 22 11 11 8 12 8 7 6 5 3 13 19 2 EHEC 0157 MLVA No 22 2014 13 07 08 10 14 16 PFGE TN g501 8 1 13 13 0O157 3 1 PFGE IS 13 21 22 13 06 15 1 WI 1 PFGE IS 13 37 13 04 1 13 01 03 2 13 09 35 13 06 15 1 2013 EHEC O157 23 PFGE IS MLVA
15. S IS PFGE 8 IS PFGE IS PFGE 13 07 08 10 14 16 1 st 2012 O157 IS 9 MLVA PFGE IS MLVA 3 IS MLVA PFGE 3
16. ystem MLVA EHEC 3000 4000 1 HUS 1 2 EHEC 0 2013 0157 52 O26 25 O111 7 2 1 2013 EHEC O157 59 EHEC O157 PFGE
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