(57)【要約】 本発明は新規なセルラーゼ核酸配列、指定のBagC el、及び
Contents
1. 10 20 30 50 2007 525179 2007 9 6 24 10 20 30 50 100 10002. 10 20 30 50 2007 525179 2007 9 6 16 10 20 30 1000 1000 100 100 10000000000000000 1000000000000000
3. 10 0 RE r m oe a 10 20 30 40 50 2007 525179 2007 9 6 22 10 20 0 100 0 1000 10000 0 10000000 1000 100 100 0
4. 2007 525179 2007 9 6 26 10 20 00000000000000 000000000000 nnnnnonnnonn nnnnnnnnnnnnnan 30 50
5. 2007 525179 2007 9 6 27 10 20 30 50 100 100 SE 1000
6. amp 8 H HD Z H A HD H Ot HD HD HD HD HD HD HD H 5 O HD O 5 5 5 50
7. 0 0000000000000000 00 74 100120145 OO 74 100122839 74 100133547 72 00000 72000 1000 000 13000 2 0000000000000000000020000000000 000 2000 00000 DD DO OD DDD DDD OOC DO 0000 U 0 28150 002 ABI 48024 AAO3 5 BALI DW7 DOS EAD4 4 4B050 03 48063 018 0005 QM 832 AB 866 75 536 48064 AFO2 AFO4 AF11 024 6 9 31 2007 525179 2007 9 6 48065 5 26 AAJOY AA72X 801 ACI4 18 BDLS CA31 955 CA57 4003
8. 50 17 2007 525179 2007 9 6
9. 28 2007 525179 2007 9 6 Figure Nucleotide sequence of inserted environmental DNA Figure 1B TGCCACAAAG TAAAGTTAAA 50 TGACAAAAGT ACGGATGTGA TTCAGCAAGG GGAGAAATTA 2500 TGGAACGAAT GATAACGAAA 100 AATGAACGTG ATATAACCCA 2550 GATGAACACG TGAAAGAGGG 150 2600 TIGTGACAAG AAAGGGTGTT ARATGAAGAT CAAAGCTAAA 200 ATATTAAAAG GGGGCGTTCT 2650 TAGTATATGA 250 AAGGACGGAA AGCCAATTAG 2700 300 AGATCAAGAT ATACAGCTTT ACTTAAACGG AAAT
10. 100000000000000000000000 50 2007 525179 2007 9 6 6 1000 100000 100 10 10000000 1000000 10000 20
11. 2007 525179 2007 9 6 12 10 20 30 50 0000000000 nnnennnnnn C C C 2007 525179 2007 9 6 13
12. 20 30 50 1000 1000 100 JP 2007 525179 9 6 9 10 20 1000
13. 30 100 50 100 100 000000000000 10000000 100 00000000000000000000000000000000 15 2007 525179 2007 9 6
14. 10 20 30 nnnnnn JP 2007 525179 9 6 14 1000000 10 20
15. 1000000 1000000 10000000000000000 100 30 50 100 1000 1000 JP 2007 525179 9 6 8 10
16. i 2007 525179 2007 9 6 10 10 20 30 50 BARA 2007 525179 2007 9 6 11
17. u 10 20 30 50 2007 525179 2007 9 6 nnmnnn n 4 n 10 20 30 50 2007 525179 2007 9 6 19 10
18. 20 30 50 JP 2007 525179 9 6 4 10 20 100 1000 1000
19. 30 1000000 000 100 10000 100000 1000000 0000000000000 00000000000000 0000000000000000000000000 50 2007 525179 2007 9 6 7 1000000 10 0000000 000000000000000 20
20. TGGAAGCTGT 1590 AGCGGAGAGA CGGTGGAATA AAAAATGGCA ATTATGITCA 1700 AGGTAGACGG TAA 1713 2 JP 2007 525179 9 6 10000000 C C 10000 10000 0010 0010 000 10 20 30 50 2007 525179 2007 9 6 3 10
21. 800 ATACCTTACG TAAAAATGGC AATTATGTTC AAGGTAGACG GTAACATGAT 3250 GAGAAGAAAC GCTACCGTGG CGTCATTGAC ATCGAATAAC GAAGCAGAAT 850 AGATAAACCA GCCTACCTAT CGTTTTTGGA AGAAGGCAAA 3300 ATGCCGCTTT ACATTTAGGA CTTAAAGAAC TTGAAGGGAT CGGTGCGCAT 900 GAACTTTAGA AGTGACGGTG 3350 CGGTGATTCT 950 3400 AAAAGGAGAA TGGGCGIGTA TGGAGGAGGT TGGGCTGACC 1000 TATTCAGGAG CTATGAATGC ATATAGTCTC 3450 GTATTGATCA GCATTTAGCT AAATTAGGCA TGACCGCTAC TTATAAGTTA 1050 TGACGAATAA AAAGAAGTGA GCCTAGTCCC CGAGGGGAAG 3500 ATCCCCCGTA AAGAAAACCG TGAAGCAGAT CACAAGCGTT 1100 GGGATAATGC CAACGTATTG GATTAAAGTA CCTTCTTGAT AAAAAGAAAG 3550 AAACGGGCAA GAAATTATAA TGTCAGTGAG CGTGGTGCAG 1150 GGTTTTCAAG AGATGGAAAT GGGCICGTTT AATTACGCCT 3600 ATTAGTCTGC ACCCGCATAA 1200 ACATCATAAA 3650 TAAAGGAACG TAAGCAAGCG TIGCGACAGC 1250 GTTCGATGGC
22. 0 100000000000000000000000 100 100000 100 10000 1000 100 30 100 1000000000000 100 100000 50 100 1000 100 23 JP 2007 525179 9 6
23. 10 20 100 30 100 0000000 1000 100 100 000000000000000000000000000000 00000000000000000000000000000000 2007 525179 2007 9 6
24. GACGCTGTTG GAGATGATGA 200 00000 0000000000000000 CIGASGNADO 300 5 2 GAAGATTATA TCAATCGGGT 350 00000 00000000000000000 AAAA Taler RICATTO ACNTAACTAT di I000000000000000000000000 TOQGAACAAT TOTCOGAMMA 600 GAAGATTTAA ATAAGACGTT CAGGAGGCAR 650 10000 CGCCCTTTAG GATAGAAACA 700 TOGCTTG TGATCCT 750 TATTTAATIG 800 850 CNGCGCGTGG 500 GTATTAGGCG GITAGGCITT GACAAAAGTA CGGATGTGAT 950 GAGRAATTAA CATCATCTCA 1000 TATAACCCAT ATAACGGCCA 1050 CGAGAAACTT TGATCAAGAA 1100 GGGCGTTCTG 1150 GCCAATTAGA GATCAAGATA TACAGCTTTA CTTAAACGGA 1200 ARTGAGCTAA CAGCCTTACA GAATCGCTIG TTCTAGGAGA 1250 1300 TACCCCTGGT 1350 CAATTIAA CAATTACAGA 1400 GCGATCCCTA 1450
25. 10000000000000000 1000000000000000 1000 100 2007 525179 2007 9 6 25 10 20 30
26. 3700 AAGTGGCAGT 1300 3750 TTTTTGACTA ACTCCTACTT 1350 CTGTAGATTT TACACCAAGT GATGATTITT TGACCCTTTT 3800 AATAATCACT AATTTTGACG 1400 TGGTGCAACT GATGATTATT 3850 ATAAACTAGT AACGATGTAG 1450 TCTATTGAAC AGAAAAAAAA 3900 TTGACCATAT AAGAAGACIC 1500 TCATGCCAAA TGCTGATATG AGTGCGTATA 3950 TTTGAAAGGT GGAGAGGACA CATGGGTTAT ACCAAAGCGA 1550 CGTTACIGGA TAAGGTGCTG CCTGAGTTTA TGAGAGCAAC 4000 AGTGTACGTT GAAAAAAACT GTCTTGTTTG GTTTAATTCT 1600 TCTCAGCACC GTGAAGACAC CTTTGCAGCT 4050 GAAGATGTCA CTTCGTCACA 1650 TGGGAGGATA CGGGGCTTTT AAATTGGCGC TGCGGCAACC GGAACGCTTC 4100 GTIGGATATT CACTCCTATG TAGCTGACAT GCAGCCTGGC TGGAATTTAG 1700 SCTGCAGCTG TGTCATTATC TGGTGCAGTT AAGCAAGTCA 4150 G
27. 100 00000000000000000000000000000000 30 100 0000 00000000000000 50 JP 2007 525179 9 6 5 10 20 30 100
28. 2007 525179 2007 9 6 19 RR FF A 1 33 2007 525179 P2007 525179A 43 281949869 2007 9 6 51 Int Cl Fl 38 C12N 9 2006 01 C12N 15 00 ZNAA 2B150 12 1 15 2006 01 C12N 1 15 4B024 C12N 1 19 2006 01 C12N 1 19 4 12 1 21 2006 01 C12N 1 21 4B063 12 5 10 2006 01 C12N 5 00 48064 A 3 R BRIM 12 88 2006 513418 P2006 513418 71 HERA 500284580 86 22 16 4 28 2004 4 28 Vibvia A173 3FN iy 85 MARS HEH A 17 12 2 2005 12 2 2 86 ER HME 52004 013175 97 87 W02004 099369 925 87 16 11 18 2004 11 18 74 R A 100071010 31 60 467 255 Fer TE 32 15 4 30 2003 4 30 100121762 83 US FET BU 100126767 FET 74 100118647 74 100138519 Fer EF RRAS 54 HRAB 570000 000000000000000000000000 CCAAAGCGAA 50 0000000000000000000000000 0 GGAATITAGG
29. 20 30 50 2007 525179 2007 9 6 20
30. 0 TGTTCCAATG ACATCAGCTG 100 QPGWNLGNTF DAVGDDETAW GNPRVTRELI KTIADEGYKS IRIPVTWONO 100 TTCGTCACAG TTGGATATTC AGCTGACATG 150 MGGSPDYTIN EDYINRVEQA IDWALEEDLY VMLNVHHDSW LWMYDMEHNY 150 CAGCCTGGCT GGAATTTAGG AAATACGTTT GACGCTGTTG 200 DEVMARYTAI WEQLSEKFKS HSHKLMFESV NEPRFTQEWG EIQENHHAYL 200 AACAGCGTGG 250 EDLNKTFYYI VRESGGNNVE RPLVLPTIET ATSQDLLDRL YQTMEDLDDP 250 CTGATGAAGG GTATAAAAGC CAGTGACATG 300 YLIATVHYYG FWPFSVNIAG YTHFEQETQQ DIIDTFDRVH NTFTARGVPV 300 ATGGGTGGTT CTCCAGATTA TACGATAAAT TCAATCGGGT 350 VLGEFGLLGF DKSTDVIQQG EKLKFFEFLI HHLNERDITH MLWDNGQHLN 350 ATAGATTGGG AGACTTATAT GTGATGTTAA 400 RETYAWYDQE FHDILKASWE GRSATAESNL IHVKDGKPIR DQDIQLYLNG 400 ATGTGCATCA TGACTCATGG CTGTGGATGT ATGATATGGA ACATAACTAT 450 NELTALQAGE ESLVLGEDYE LAGGVLTLKA DTLTRLITPG 450 TGGCAAGATA TACAGCTATT 500 QENSGADWRF QLONVDVPTV ENTDGSTWHF AIPTHFNGDS LATMEAVYAN 500 ATTCAAAAGC AATGAGCCTA 550 GEYAGPODWT SFKEFGEAFS PNYATGEIII SEAFFNAVRD DDIHLTFHFW 550 GGAGTGGGGA GAGATTCAAG ARAATCATCA 600 SGETVEY
31. AAATACGTT TGACGCTGTT AAACAGCGTG GGGGAATCCT 1750 ACCAGACTCC ATGCCTTTGG TGAAGGGACG AAAATCGCAG 4200 CGIGTAACAA GAGAGTTAAT AAAAACGATT GCTGATGAAG GGTATAAAAG 1800 GGACA 4205 CATTCGTATC AATGGGTGGT TCTCCAGATT 1850 ATACGATAAA TGAAGATTAT ATCAATCGGG TGGAGCAAGC GATAGATTGG 1900 GCGTTGGAGG AAGACTTATA ATGACTCATG 1950 GCIGTGGATG TATGATATGG AACATAACTA TGATGAGGTC ATGGCAAGAT 2000 TIGGGAACAA TTGTCGGAAA AATTCAAAAG 2050 CAATGAGCCT AGGTTTACGC 66 2100 AGAGATTCAA GAAAATCATC ATGCTTACTT 2150 TCTATTATAT TGTCAGAGAG TCAGGAGGCA ATAATGTGGA GCGCCCTTTA 2200 GTATTGCCTA CGATAGAAAC AGCCACGTCT CAGGATTTAC TAGATCGCTT 2250 GTATCAAACA ATGGAAGACT TGGATGATCC TTATTTAATT GCCACGGTGC 2300 CITCIGGCCA ATATAGCAGG GTACACTCAT 2350 AAACACAACA AGATATTATA GACACCTTTG ACCGTGTTCA 2400 ACAGCGCGTG GAATTCGGTT 2450 29 JP 2007 525179 2007 9 6 1000 Figure2 ORF Nucleotide sequence of cellulase gene Figure3 Amino acid sequence of BagCel cellulase AAAAAAACTG TCTTGITTGG 50 KKTVLFGLIL CLSVSMFVPM TSAEDVTSSQ LDIHSYVADM 5
32. AACATT TCATTTTTGG 1650 AGCGGAGAGA CGGTGGAATA TACCTTACGT AAAAATGGCA ATTATGTTCA 1700 AGGTAGACGG TAA 1713 SDInt a 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 0000 81 9 42 708 1 68 3386 742 326 732 1 165 3 02 1 07 2006 01 2006 01 2006 01 2006 01 2006 01 2006 01 2006 01 2006 01 2006 01 2006 01 2006 01 30 JP 2007 525179 2007 9 6 942 0000 000 708 00000 19 00 68 3 386 00 7 42 3 26 1 7 32 1 165 3 02 42 1 07 LS SD SL SZ TZ UG ZM ZW EX AZ BY KG KZ M RUT EP AT BG CH CY CZ ES Fl HJ 1 E IT LU MC PL PT SE 5 SK TR BF GG N M NE SN TG AG AL AZ BBY DK EG ES Fl Q H HJI D IL IN IS JP KP KR LG LK LR LS LT LU LV M MG NA NQ OM PG PH PL PT RU SG SD SE 56 SK SL SY TZ UA UG UZ VG WN YU ZA
33. GAGCTA 2750 CGTTTGAAGA TCTAATGATG CGAGAGGTTT 350 AGGCAGGGGA GGAATCGCTT GTTCTAGGAG AGGATTATGA 2800 TATCACTTTG GAGCGGAAGA 400 GGCGTATTAA CGCTAAAAGC ACAAGACTAA TTACCCCTGG 2850 TTGGTGTTAT TGTGTATTTA AAAGAAAGGG AAACGAAAAA AATGGTTAAA 450 TCAATTAGGA ACCAATGCAG TCATCACAGC ACAATTTAAT TCTGGAGCAG 2990 AAACAATGAA 500 ACTGGCGTTT TCAATTACAG AATGTGGACG TGCCAACGGT CGAAAATACA 2950 GAAGTGACAT ACTGCACGAC TAAAGGTATT CGAACAACCT TTCATTCAGA 550 GATGGCTCAA 3000 CGCAGAAGAT TTTCAGCGGA 600 TCTTGCGACG ATGGAAGCTG TTTATGCAAA CGGAGAATAT GCTGGGCCGC 3050 AAAACAGATC ATGAGCGTGA 650 GAATTTGGCG AGGCGTTTTC 3100 CGGAGCCGCA 700 GCCACAGGGG CGGTACGGGA 3150 GIGTATITIG ATGGGGGATT TGATTTGGAG ACACAACGAT 750 TGATGATATC GAGCGGAGAG ACGGTGGAAT 3200 CTGGTCITGG GTGTGATTTA
34. TLR KNGNYVOGRR 570 CAGGAGGCAA 650 CGCCCTTTAG GCCACGTCTC 700 AGGATTTACT AGATCGCTTG TATCAAACAA TGGAAGACTT GGATGATCCT 750 TTATTATGGC TTCTGGCCAT TTAGTGTCAA 800 TATAGCAGGG TACACTCATT TTGAACAGGA AACACAACAA GATATTATAG 850 ACACCTTTGA AACACATTTA CAGCGCGTGG TGTCCCAGTT 900 GTATTAGGCG GTTAGGCTTT GACAAAAGTA CGGATGTGAT 950 TCAGCAAGGG GAGAAATTAA 1000 ATGAACGTGA TATAACCCAT ATGTTATGGG ATAACGGCCA GCATTTAAAT 1050 ATGCATGGTA TGATCAAGAA TATTAAAAGC 1100 GAGTTGGGAG GGGCGTTCTG CTACAGCAGA GICTAATTTG ATTCATGTGA 1150 AGGACGGAAA GCCAATTAGA GATCAAGATA CTTAAACGGA 1200 AATGAGCTAA CAGCCTTACA GGCAGGGGAG TTCTAGGAGA 1250 GGATTATGAA CTAGCAGGAG GCGTATTAAC GCTAAAAGCG GACACCCTCA 1300 CAAGACTAAT TACCCCTGGT CAATTAGGAA CCAATGCAGT CATCACAGCA 1350 CAATTTAATT CIGGAGCAGA CTGGCGTTTT ATGTGGACGT 1400 GCCAACGGTC GAAAATACAG ATGGCTCAAC GCGATCCCTA 1450 TGGTGATAGT CITGCGACGA TGGAAGCIGT TTATGCAAAC 1500 GGAGAATATG CTGGGCCGCA AGATTGGACG TCATTTAAAG AATTTGGCGA 1550 GGCGTTTTCT CCTAATTACG CCACAGGGGA AATTATTATA TCAGAAGCCT 1600 TCTTTAACGC GGTACGGGAT GATGATATCC ATTT
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