Xpress Plasmid PLUS Kit クイックガイド/日本語
Contents
1. E e kaa NIPPON 499 Genetics coa G FestGene Xpress Plasmid PLUS Kit FastGene LIZDLA TIAZE FIA v R IM act n atc 25Z EFDNAO 5 FG 90102P 2 preps FG 90202P 10 preps FG 90302P 25 preps IHTIE TITA ZE HSIEITA s iE HERE ml 2400 0D600f amp HAE ml 800 0D600 1 td 6 000x g 4C 15min 6 000xg 4C 15min X ERA EE BRA Beas lOml yD P PXI 3ULT wZA 2OmIIyIP PXI MILF YIR Y 558 10ml ty27 PX2 8226 1018 20m1 tv27 PX2 S81 56 108 58 5min jm Smin E f 0 20ml 27 PEQ 20ml Jty77 PEQ 3 ET 1Oml JtZ27 PX3 812 46 1018 20ml ty27 PX3 E 46 101 ly 58 Smin jm Smin D k y GALA 3E TI IVURAZJI LSe hno RN id Ya q SK Oml C6 Z27 PEQ LE Eo TOUBEZ 41 9 0PE t 5ml J6yZ27 PW d m H isl Oml 69 27 PE y a 75ml 4Y TOD Jb B38 10ml tyZ27 PEDO 75f48 p RE AR A8150 or AJULTYIA y 2m 2min 5 000 xg 4C 30min 15 000x8 AR MOA lS MERAS ECHR lt IES 5m 7096T47 Jv GER I 5 000 xg 4C 10min v EJE 10min 15 OOOxeAMOBAlS MERAS ETERNE HE EJVYTP or MARK Y X1 TNDON YI EEN SSD SE Cd X2 PREI YI 7 FG 90002 buc gs TO fees BALITT42ABURA ES httpJ www n genetics com m 5 MAA A NIPPON GPRS Genetics co G FastGene Xpress Plasmid PLUS Kit Purification of high 82. e X2 need additional buffer FG 90002 in the beginning of low copy plasmid protocol ge NIPPON GENETICS EUROPE GmbH cd English web page www nippongenetics eu German web page www nippongenetics de
3. low copy plasmid DNA FG 90102P 2 preps FG 90202P 10 preps FG 90302P 25 preps High copy Plasmid Low copy Plasmid Cultivate Culture Vol ml 400 OD600 Culture Vol ml 800 OD600 Harvest of i estes 6 000xg 4 C 15min 6 000xg 4 C 15min cells X Remove the supernatant Remove the supernatant Resuspension 10ml of PX1 Vortexing 20ml of PX1 Vortexing v Cell lysis 10ml of PX2 Invert the tube 6 10 times 20ml of PX2 Invert the tube 6 10 times y ley 5min at room temperature 5min at room temperature Equilibration x sa q SK 20ml of PEQ 20ml of PEQ Thimble MA Neutralization 10ml of PX3 Invert the tube 6 10 times 20ml of PX3 Invert the tube 6 10 times y 5min at room temperature 5min at room temperature Clarification ud Invert the tube 3 times x d Load the lysate on Thimble of the lysate y ENEE d gt AE 10ml of PEQ Discard Thimble 2nd Washing 15ml of PW Y Elution 10ml of PE y 7 5ml Isopropanol RT 0 75 x isopropanol to 10ml of PE Precipitation Invert the tube 15 times Or Vortexing 2min at room temperature 15 000 xg 4 C 30min If in case of less than 15 000xg please read the kit protocol 0 Wash amp Dry 5ml Lais ethanol RT DNA pellet 15 000 xg 4 C 10min Y 10min at room temperature If in case of less than 15 000xg please read the kit protocol Reconstitute Dissolve the DNA pellet in TE Buffer or sterile H20 DNA v 3X1 need preparation before us
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