カラム管理上の注意点
Contents
1. 1 2 1 1 Pyridine 1 Procainamide Hydrochloride 1 Acetylacetone 2 Phenol 2 Phenol 2 Phenol 3 8 Quinolinol 4 N Benzoyl N phenylhydroxylamine 5 Benzene 1 2 2 a 0 4 8 min 0 5 10 min i 5 10 5 20 min Column Wakopak Wakosil I 5C18 RS Column Wakopak Wakosil I 5C18 RS Column Wakopak Wakosil I 5C18 HG 4 6mmx150mm 4 6mmx150mm 4 6mmx150mm Eluent CHsCN Hz0 30 70 v v Eluent CHsOH 20mM KsHPO4 Eluent CHsCN 20mM KsHPO4 Flow Rate 1 0ml min at 35 C pH7 6 40 60 v v pH7 6 40 60 v v Press 50kg cm Flow Rate 1 0ml min at 30C Flow Rate 1 0ml min at 30C Detection UV 254nm Detection UV2. 66 V TYP FPP 68 PP PP PPP PPP YYrFTTT 69 ENS 70 V rr 81 M Presep VI 1 eenseeeeseeeeeeeeseeceeeeeeeeeeeeneeeeeeeeeeeeeeeeee 82 VI 2 NH FPP 84 VI 3 Presep PT 86 VI VI 1 Or 88 VI 2 CCPTF PP 89 VI 93 oo 94 VI RR 2 97 EE 36
3. 39 2 im
4. 4 10 50C 5 AR 1 4 1 2 50 50 v Y 3 4 5
5. 2 5 7 10 1 0 1M EDTA 2Na gt 2 1009
6. 1 Wakopak Column Performance Report 10 1 2 Wakopak Certificate of Analysis 1 2 Wakopak Column Performance Report Performance Report wakopak Co i i ey i Cee Naphthalene en ile Naphthalene feettore 5 ET Naphthalene Benzene Naphthalene 1096
7. 2 0 1 0 2 3 HPLC 3 1 HPLC 2 pH 2 5 7 Wakosil AR pH2 8 3
8. 1 Wakosil Wakosil Wakopak
9. SIL Fluofix Fluofix IL 2 1 1 2 3 HPLC
10. 0 ud 2286 1 DMSO 200 ul 2 10 3 0 1 EN SEM MG 30 a 2
11. HPLC 0 1 0 2ml min 5 10 6 1 2 7 1 THF
12. 0 1ml min HPLC 1ml min 0 45 m HPLC 180 28 2 1 15 20
13. 4 I I 1 ee 32 I 2 ee 33 I 3 ee 33 I PTI TNI RE ROT NRVC NS CeO RP OR RN 34 36 TT 1 CPP 3b 2 ee 38 IV HPLC Wakopak V TTY 40 IV 2 59 NV 3 TYP PF 51 V 4 UHPLC RPCPPPFPYP PF 62 V 5 HILIC FT 64 V 6 CCP
14. Test Chrosatosraa Inspection Data Performasce Ressit Wakosil 5C18 RS Lot No _9542 ier A SK Keight Se Li 10 Helight ctor es Separation factor TeTaiting Factor Asymaetry factor Tested by K Shibats Wako Pure Chemicea Industries itd Wakopak Certificate of Analysis 2 Coneaens Cowrw Size mmn 250mm Fow Fiate OU Crorqkogram 2 5 f Wakopak Certificate of Analysis Wakosil 5C18 RS Lol No _9542_ oe Mk i ODS EE th B Yon own cA 000 4 7 Naphthalene Naphthalene Naphthalene Benzene Naphthalene 1096 Pyridine Phenol Procainamide amp 2 4 6 TriImethyl
15. 254nm Detection UV 254nm 0 16AUFS 5 0 5 0 Naphthalene KAve 3 79 Std dev 0 13 Naphthalene K Ave 3 91 Std dev 0 0 075 4 0 4 0 J ooooo oooroooooooooO OS ooo_oo_ooo o_o 3 0 a 3 0 a Naphthalene Benzene Ave 2 08 Std dev 0 008 gt a Naphthalene Benzene Ave 2 09 Std dev 0 006 2 0 ee ese ee 2 0 J ooooooooOooooooooooO_OOro oo_oo o_o_ooOO oo 1 0 1 0 1 1 1 0 5 10 15 20 0 10 20 30 40 Batch Number Column Number Column Wakopak Wakosil I 5C18 HG 4 6mm x 150mm Column Wakopak Wakosil I 5C18 HG 4 6mm x 150mm Eluent CHsCN HzO 60 40 V y Eluent CHsCN HzO 60 40 v v Flow Rate 1 0ml min Flow Rate 1 0ml min Sample Uracil Benzene Naphthalene Sample Uracil Benzene Naphthalene p 71 GMP Wakosil I 3C18 HG RS AR Wakosil II 3C8 RS 3 m Wakosil I 5C18 HG RS AR Wakosile T 5C8 HG RS 5um Handy ODS
16. pyridine 6 Dopamine amp DOPA Oxine Cu EE VE Triphenylene Dibenz a h anthracene p 71 38 Wakopak EN C18 C8 C4 TMS Ph PTH PTH 20 CN PTC PTC 17 NHz DNA AS AQ Cu SO MCPP SAX 5 UMP Agri 9 TPN SCX GP N6 PAHs AS Aqua Diol DNPH I DNPH SPP Re 8
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