BIAevaluation （Ver.2.1） 取扱説明書
Contents
1. OANA HE SP S EBmQ r lt e5X33J2 Pe2. OFF TEL 03 5331 9336 ERIT RE U s d 9 00 17 30 WWwW gelifesciences co Jp e mail Web 2009 GE
3. GE 169 0073 3 25 1 TEL 03 5331 9336 FAX 03 5331 9370 e mail Tech JP ge com ISO 9001 2000
4. NHS EDC DSAI DSA DSA 7 7 LU 2 r V O KEYWORD ng ml Excel ug ml nM uM MAIN BIAevaluation Software Handbook 49
5. File gt Open i Interaction between analyte amd immobilized ligand Ru Caontral LAU CO EJ Lh wre i BlAevaluation Software Handbook ver 45 3 epe ee 1 GLENA ret 1 n p 3 Operation Henlace Original C Zero at Average of Selection C Zero at Median of Selection Add As New C Curve Value C Curve Value Cancel C Ln Curve C 1 7 Curve G Ln TU 7 Y at Cursor Position Curve Curve 2 Blank Run Subtraction Curve 2 5 GLCNAC FE 1 2 Apply to Entire Working 5et Curve Curve Blank Run Subtraction Curve 2
6. Plot 4 a X RII 900 700 500 Response 300 0 Plot 1 n XJ RU 1200 1000 BIAevaluation Softwore Handbook ver2 9 1 01 his txt amp Project ml gt x Id Hame 5nurce his_trt 1 HI5_T gt xT Plot 1 x 15000 14500 14000 13500 13000 12500 12000 500 1000 1500 2000 3 1 3 1 10 BIAe
7. Ea BIAevaluation Software Handbook ver2 29 2 Plot 1 x n u Eus ui 2 8 600 650 RU 5 i 55 NR ERE DE V V S POLI dx paie d T E f m E 10 Cuveld C jt lkd apr22a blr 7 apr22a blr B lapr22a blr 9 lapr22a blr 10 japr22a blr 11 qM fM pM nM uM mM M kM 30 BlAevaluation Software Handbook ver2 2 i a _ nl x RU 110 100 au au 70 B Response SH 40 3l 20 Curve ld 22 22 8 22 9 22 10 22 11 k k
8. Replace Original Add As New MU ti File gt Close 44 BIAevaluation Software Handbook 3 WMl1 fL oos 2ezs8L ene RU 200 150 100 504 05 50 4 100 150 Average Req Eq Heg eme v ceri f Zl p 150 i E E Selection X12143 5 Y 1271 7348 70 6952 Fit Non Linear Fit BIAevaluation Software Handbook 45 3 Options 3 Drift 3 ffset Description
9. 1 1 Plot 1 x RU Response c C a A A A 450 550 650 Time amp _ 6 150 200 250 300 350 400 450 500 550 But 650 Th Time am In abs dY dX v 2 0 TR 9 SS EIRIO DSE pouzi C wna O Ea 25 2 2BL C 8 NCOJ Z2IC S 01111 BIAevaluation Software Handbook ver2 35 2 Plot 1 B RU Response v gn P AERA Tires i Lis PE eri res spun ni ES Jj V a Eu EC is Constants X AB type 3 Fit Cancel Cuveld C n t 22 Fit Tolerance 22 8 22 9 0 0005 22 10 22 11 Apply To Entire Working 5et 30 Fit 36 BIAevaluation Software Handbook 2 ATB AB type 3 Fitting z lolx RU 100 50 80 70 B 50 40 30 20 10 300 320 340 360 360 400 42
10. Rea F ka 1x105 1 5 1 ka 1x105 1 5 1 1x10 1 5 1 1x10 3 5 1 association phase dissociation phase Req CC Ko Reg bulk effect 40 BIAevaluation Software Handbook 3 RU 1350 1300 1250 1200 4 1150 Response 1100 1050 1000 950 i 0 100 200 300 400 500 600 700 ime Req C ca RU Req ver C Req t t t 0 1 7 2 7 7 4
11. 2 3 12 BIAevaluation Software Handbook ver2 1 Project nl x ld Hame 5Snurce 1 his txt 1 5 2 his txt 1 Clipboard Paste 3 his txt 1 Clipboard Paste 4 his txt 1 Clipboard Paste 3 le Plot 3 x 1400 1200 1000 ang 600 400 200 BIAevaluation Softwore Handbook ver2 13 1 1 5 Plot 1 B X p 100 a u 60 Response 40 20 amp PEPTIDE BLE x ld Hame t nurce w 1 sample 1 PEPTIDE BLE control PEPTIDE BLE 1 2 14 BIAevaluation Software Handbook ver2 1
12. DEFINE APROG conc PARAM KEYWORD KEYWORD CAPTION FLOW INJECT 0 10 RPOINT 5 20 INJECT 1 50 END MAIN END FLOWCELL APROG APROG APROG APROG APROG APROG APROG APROG APROG APROG APROG APROG APROG APROG APROG APROG APROG APROG conc conc conc conc conc conc conc conc conc conc conc conc conc conc conc conc conc conc APPEND IlD conc sample ID ng ml Binding of 96lD 96conc 5 pos 15 Baseline b bound R2F3 5 Regeneration 1 2 5 4 RZA1 DSA RZAZ DSA 2 3 DSA 2 4 DSA R2B1 IgG R2B2 IgG R2B3 IgG R2B4 IgG 2 1 AFP R2C2 AFP R2C5 R2C4 R2D1 Sample R2D2 Sample R2D3 Sample R2D4 Sample R2D5 Sample R2D6 Sample CONTINUE BlAevaluation Software Handbook ISample Iregeneration solution 10 000 5 000 2 500 1 250 10 000 5 000 2 500 1 250 10 000 5 000 2 500 1 250 uk1 1 10 uk1 1 20 uk2 1 10 uk2 1 20 uk3 1 10 uk3 1 20 55 BIAconcentration Evaluation 1 1 1 2 A BIA Concentration Evaluation A amp SSAY3 BLR La File Edit Data Window ace rra aa 24498 5 BASELINE 10 000 265847 A BASELINE 10 000 15907 1 6 BASELINE 10 000 142955 1 BASELINE 10 000 24529 3 9 boundD 5A 10 000 290208 1
13. Biacore control software El 51 lt lamia FO Start gt BIAprogram BlAevaluation 2 1 2 2 BQBIA Evaluation 2 3 9 9 gt Eie Edit Wiew Fit Window Help Project jede xe Id 1 2 Open 2 x T 22 bazickin tzt i 9 biaewal y bE kinetics tst m1 bl Iri BI amp Files E S6 6 rn fa BIAevaluation Software Handbook ver2 1 Open KINE TICS BER File Description File Type HIAInnue Result File Created By BlAlngue Version 1 08 Sensorgram Fc 4 Sensordgram Fc 4 gensorgram E m Fc 4 Use Original Colour Cancel X Sensorgram Fc 4 Hun Date HH7A Processing Unit Instrument Id Keyword APROG V alue M Use Original Color Biacore control software 1 2 k
14. FE Linear Fitting D X D D45 Curve ld ver Ks Fa N 1 b3e3 2 41 3 1 49e 4 Slope ka Icpt ky cpt Non Linear Fitting BIAevaluation Software Handbook ver2 39 3 3 5 Ko Affinity Biqcore ka
15. DEFINE APROG conc PARAM pos conc KEYWORD ng ml conc CAPTION Binding of DSA 96conc ng ml on Asialofetuin FLOW 5 FLOWPATH 1 INJECT pos 15 ISomple 0 10 RPOINT Boseline b 5 20 RPOINT bound INJECT R2F3 5 Iregeneration solution EXTRACLEAN 1 50 Regeneration END MAIN FLOWCELL 1 APROG conc 2 1 10 000 APROG conc 2 2 5 000 APROG conc R2A5 2 500 APROG conc R2A4 1 250 APROG conc R2A5 6025 APROG conc R281 uk1 10 APROG conc R282 uk1 20 APROG conc R281 uk1 10 APROG conc R282 uk1 20 APROG conc R281 uk1 10 APROG conc R282 uk1 20 APPEND CONTINUE END uk1 10 uk1 10 uk unkown C PRE 10 3 50 BIAevaluation Software Handbook 4 2 BlAconcentration Evaluation xd BIAconcentration Evaluation
16. Plot 1 _ x RU 160 140 120 100 a Response 40 20 D 20 200 250 200 350 400 450 500 550 600 650 View Split View cpliE view X 5moothing Factor P at X Position 452 E Cancel Split Screen C C dY z Ini abs dX In YO 7 Y In YO Y OK 24 BIAevaluation Software Handbook 2 Plot 1 Response 150 250 RET Time In YO R0722 TERED SMAA IRADAS YERI YI LRL E 8 Fit Dissociation Kinetics Constants AB A B x Fit Cancel Cuveld RO Ro apr22ablr 7 Rar Fit Tolerance 22 8 22 9 0 0005 22 10 22 11 Apply To Entire Working Fit BIAevaluation Software Handbook ver2 25 2 AB A B Fitting _ e RLI 135 13 125 m n c a un Curve 19 22 7 3 25e h 0 144 aprzZa hlr 8 3 21e h 1 0 136 aprzZa hlr 9 1 53e h 1 0 0342 2 2
17. Uo 529 377 1 FE Lqg2 plot Lag1 plot BIAevaluation Software Handbook ver2 27 S OD EB i 2 ka Window gt Plot 1 Plot 1 _ x In Y Y View Split View cpliE view X 5moothing Factor 5 at X Position 452 iz EL Lancel Split Screen C C dY 7 dX Ini abs d lt Oin YU 7 Y 2 In abs dV d OK 28 BIAevaluation Software Handbook 2 1 1 Plot 1 x RU 140 100 a B 20 20 150 250 450 550 50 Time RLI 2 E z B m 1 150 200 250 300 350 400 450 500 550 eun 650 Time
18. i BlAevaluation Software Handbook ver 47 3 AB at steady state x v Cuveld n Respvsc Q Apply To Entire Working Set FO APR Eit Cancel Fit Tolerance Bus 22 7 36 7 de 7 52 7 Ta T ge r 7 Curve Id K SEIK Hmaw SEIRmal n Chi 2 Resp vs c 1 eb 94 8 11 4 1 14 6 Fitting 5122 000000023e 7 amp 2 8 000000093 CC C KA 3 73x106 M1 07730 Ko 1 2 7x107 M 48 BIAevaluation Software Handbook 4 Biacore 4 1
19. 4 3 DSAllgG AFP sample uk1 1 10 uk1 1 10 uk unkownj 1 10 54 BIAevaluation Software Handbook ver2 2
20. BIAevaluation Software Handbook ver2 5 1 Y ranstorm X peration Replace Original Zero at Average of Selection C Zero at Median of Selection Add As Hew Curve Value Yalue 1 C Curve Value izi Cancel Ln Curve 1 7 Curve GC Ln TU Z Y at Cursor Position O Curve Curve 2 Blank Run Subtraction Curve 2 1 KINETICS 4 1 Apply to Entire Working 5et O Zero at Average of Selection Replace Original amp 9 Plot 4 D X FU 1200 1000 200 Response 200 6 BIAevaluation Software Handbook 1 Plot 3 D X RII 900 700 500 Response 200 100 100 100 200 300 400 500 Bun r X dl amp lsingte overtay Plot
21. EILAN QU 5 35 5 3U 977 0u 3 JALAN 177510 Ab IC 60 O a5 2 B S B 2 Fisch CRUS VN i x iB
22. Y ranstorm X peration Replace Original Zero at Average of Selection Zero at Median of Selection Add As Hew O Curve Value Vale C Curve Value 5 Cancel C Ln Curve C 1 2 Curve TU Z Y at Cursor Position Curve Curve 2 Blank Run Subtraction CuveZ 2cono E Apply to Entire Working 5et QCurve Curve 2 Blank Run Subtraction Curve 2 2 control Replace Original Add As New eXTEBUEE UII SEU Le gs 3 Plot 1 X pm 100 B B 40 Response 20 20 40 20 20 40 Bt Bt 100 120 140 BIAevaluation Software Handbook 15 1 1 6 View Labels Ok Protein Interaction Y Labels LlAuto La
23. Duos am Plot 3 X RU 900 700 500 100 100 100 200 300 400 500 600 700 BIAevaluation Software Handbook ver2 7 1 Plot 3 Xx RII 900 700 500 m m m 300 Calculate X Transform X ranstorm peralion Heplace Original Curve Value at Reference Line O Curve Value Add As Hew V alue C Curve Value GE Cancel Apply to Entire Working Set Curve Value at Reference Line Replace Original Plot 1 gt jon 24400 24300 24200 24100 Response 24000 23900 23800 8 BIAevaluation Software Handbook 1 tumse C Eb X Y ERARI
24. 1 5 1 6 0 2 2 1 Non Linear Fitting ka D ka 2 2 Linear Fitting 3 3 1 3 2 Average Req BIAevaluation Software Handbook ver2 16 17 19 20 21 21 25 24 28 55 55 55 34 42 43 43 45 4 4 1 49 4 2 51 51 51 52 53 54 4 3 54 54 56 BlAevaluation Software Handbook ver2 1 1 Overlay plots 1 1
25. 20 22 BIAevaluation Softwore Handbook ver2 2 Fit Preference Association Model Dietault Association Model X Cancel ptions Drift ly Offset A B1 B2 AB1 4AB2 type 2 1 2 A1B A2B Description analyte A reacts with a single site on the immobilized ligand B Calculates ka and Req Model assumes kd known A B 1 1 1 1 Type 1 2 Non Linear Fitting Type 5 4 Linear Fitting A B1 B2 AB1 2 2 1 Type 1 Type lt Non Linear Fitting A1 A2 B A1B A2B 1 2 Competition ossoy A B AB Type 1 Type 2 OK BIAevaluation Software Handbook ver2 23 2 ka
26. 2 Chi2 20 3 de Residual Plot BIAevaluation Software Handbook ver2 31 2 Residual e gt gt gt Az z gt Eri Eri gt J gt gt 300 320 340 360 3at 400 420 440 Time 2RU Lag 1 plot Lagi 2RU Uo 5 Eb Lag2 plot Log1 plot 32 BIAevaluation Software Handbook 2 2 2 Linear Fitting 2 1 Non Linear Fitting Plat 1 B x Response M Fit Preference Association Model Hodelz AB type 1 AB AB type 2 type 3 A B AB type
27. 3 d c P 2222 Use Original Color OK 3Project _ x ld Hame 5uurce KINETICS Fc 4 EINETICS BLR KINETICS Fc 4 KIHETICS BLR KINETICS Fc 4 KINETICS BLR KINETICS Fc 4 EINETICS BLR KINETICS Fc 4 KIMETICS BLR BlAevaluation Software Handbook ver2 1 1 3 d A Project al ld Shift 2 Plot 4 5 x RL 25000 23000 Response 22500 22000 21500 21000 db amp eoo0vossc n 20arcxEmencosh 2021 1 BIAevaluation Software Handbook ver2 3 1 le Plot 1 x 250
28. E boundD 5A 10 000 153 07 3 0 i boundD 5A 10 000 14294 4 I boundD 5A 10 000 24529 2 J boundl gG 10 000 290109 i 6 3 boundl gG 10 000 1537 3 boundl gG 10 000 142939 J boundlqi 10 000 245290 boundAFP 10 000 29001 4 boundAFP 10 000 153 07 3 6 boundAFP 10 000 14293 7 6 boundAFP 10 000 24435 9 3 6 Regeneration 10 000 25525_5 Regeneration 10 000 15895 7 8 6 Regeneration 10 000 14233 2 Regeneration 10 000 24753 8 E 6 BASELIHE 5 000 266196 I 5 5 000 15908 E BASELINE 5 000 143102 i BASELINE 5 000 Ta o4 G P Ra G ja Ta 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 22 s Gs DSA ESE d SG Fc ET A Id bound Concentration Evaluation 4554 Y3 BLER x 3 File Edit Data Window Help yele Fe JAPROG Lrime Window SD Slope LHSD Baseline RelResp Ild _ 1 290208 I boundD SA 28507 5 L baundD SA JFPBSR bound 5 27239 7 L I boundD SA Zb5883 2 1 boundD SA 291777 8 L 1 baundD SA 285400 bound SA Z2FB852Ff 3
29. I i boundD SA 27238 L i boundD SA 268646 i i baundD SA 3 bound SA 286546 3 I boundD SA ZFB858 5 J i boundD SA 272959 2 I _ baundD SA 266902 9 3 0 SA 26484 4 f boundD SA 276430 5 I 4 boundD SA 264046 i L L bound SA 263966 d bound SA 26379 0 I i boundD SA 276361 3 l boundD SA 263950 0 L E baundD SA 263398 baundD SA 263432 E 8 boundD SA D GD Qn Rk G 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 Sample DSA RER C NI S73 9 N C BUERU d Edit Mode amp Fi c 7172400005 Say 8889007 2 81716 OLUN 56 BIAevaluation Software Handbook Edit Edit Mode DSA Sample HE Pa LM assay 256 6 290208 18 74 10 01 0 45 2435 0 5 10 000 DSA assay 255 6 2n 285076 231038 5 55 014 1887 9 boundD5A 5 000 DSA assay 255 5 50 277825 320 1 70 0 41 Ha 11584 boundDSA 2 500 DSA assay 255 6 5 0 2 2397 0 76 0 35 0 43 652 7 boundDSA 1 250 05 assay 255 6 bl 268932 0 55 0 16 051 346 3 boundD5A 625 DSA assay 255 5 50 2
30. 10 5 0 106 22 11 3 43e h 1 104 ka 1 ka as C Ice UN 2 Chi2 20 374 37 de Residual Plot T Residual Eri E EH T gt gt T xr Time amp 26 BlAevaluation Software Handbook ver 2 2RU 4 8 Log 1 plot ABS ArrBj Fitting gt 1 5 1 2 0 9 0 5 0 3 0 3 0 6 0 9 1 2 1 5 Curve ld ISE kdl 5a apr2Z2a blr 115 29 5 5 BZe 3 251e3 122 9 18 3 0 02359 475 0 204 0 22 8 113 12 1 h le 3 1 4A7 amp 3 116 0 0141 0 0268 475 0 303 0 m Ll EDO C Ud 3 fi 5n a d 1111 I D n V 73 ATE ra lt AN 2RU
31. File Open OK ruf arid Jd IND Tal EX ok eonc rpt 25 cx evtl ee osbir x e biaeval x gE Iri Eod 0 Files 16 E c BlAevaluation Software Handbook ver2 51 A BIA Concentration Evaluation CONC RPT La File Edi Data Window 8 x Cycle APROG FC Time Window AbsResp SD Slope LRSD j Baseline RelResp ld ng mll immob 0 42693 0 13373 0 38677 BASELINE immob 0 63197 0 10028 0 67471 immoh_MAb h2micro U 0 66613 0 30586 0 45517 baseline b2micro U 0 82325 0 35906 0 59927 b2micro b2micro 3 38347 2 02958 0 36530 second Ab bZmicro U 0 55899 0 05558 0 58117 baseline b2micro U 0 66275 0 25547 0 53724 b2micro b2micro l 3 96720 2 38374 0 35373 second Ab b2micro U l 0 75142 0 25732 0 65196 baseline b2micro U 2 94728 1 68242 1 00138 second Ab b2micro 1 0429465 1 10575 0 41323 b2micro b2micro I 0 62964 1 05353 0 65703 baseline b2micro 0 50330 0 17088 0 43845 hgmicrn hgmicrn 248228 1 45732 0 59753 second Ab hgmicrn 3 0 72404 0 09957 00 74310 baseline hgmicrn 0 55753 0 08029 0 58164 hgmicrn hgmicrn I 1 63845 D 34273 0 51659 second Ab b2micro 0 69844 0 08928 0 71949 baseline b2micro 0 43175 0 00469 0 45504 b2micro hgmicrn 8 0 66538 0 04439 0 69707 second Ab b2micro 0 59952 0 07695 0 61746 baseline b2micro 0 69767 0 16346 0 57754
32. 1266 236 1b uk1 1 110 673 7 1265 607 32 uk1 1 11 682 3 1283 679 32 uk1 1 11l b81 7 1282 41h 32 uk1 1 10 b81 2 1281 364 38 uk1 1 11l 676 7 1271 904 38 uk1 1 10 brb 1 1270 544 38 uk1 1 11U 675 4 1269 174 AN 277777 1273122 58 47 uk1 1 ll 321 571 378 17 uk1 1 2l 321 570 378 47 uk1 1 l 321 1 n70 55h 33 uk1 1 ll 335 5 599 785 23 1 1 220 336 5 599 596 33 uk1 1 ll 335 3 599 219 39 uk1 1 2l 325 9 581 492 39 uk1 1 l 327 581 581 39 uk1 1 ll 327 3 582 24h Aw 328 1 8888893 583 927 217 18 uk 2 1 11U 2551 3 10029 204 18 uk 2 1 10 e542 1 9923 957 unl CIF F 7277 lt N Menu bar Window gt e Datq Overlay Plot OK BIAevaluation Software Handbook ver2 59 60 werlaw Plat Calbration Windows to Plot Calibration of A55A amp Y3 BLR View Calibration of amp 55A amp Y 3 Calibration of A55AT3 BLR C
33. 4 A B1 B2 2 type 1 2 2 type 2 1 2 A1B A2B Drift ly Offset Description analyte A reacts with a single site on the immobilized ligand B Calculates ks and r A B AB Type 3 4 OK BIAevaluation Software Handbook ver2 33 2 88 Plot 1 _ x RU 160 140 120 100 a Response 40 20 D 20 200 250 200 350 400 450 500 550 600 650 View Split View cpliE view X 5moothing Factor 5 at X Position 452 Cancel Split Screen C C dr z Ini abs dX Cin YD 7 Y 2 In abs dV d OK 34 BIAevaluation Software Handbook 2 Plot 1 lol x FAU 140 d e BD p UU ALTE ERI E 20 20 150 250 350 450 550 650 Time 5 AU c L 2 1 M Ni T B m E 1 150 200 250 300 350 400 450 s500 550 600 650
34. 6 2825 2239 7 25883 2 29177 8 2854U0 U 278276 272388 268646 29205 3 28545 3 27858 6 272992 269029 25484 4 26430 5 264046 26396 6 263 9 0 263613 263500 26339 89 26343 2 II BI II II 18 74 1 38 3 20 0 6 0 55 19 14 10 41 3 57 0 93 0 13 19 86 10 34 3 77 0 98 0 19 0 34 0 26 0 28 0 16 0 33 0 36 0 24 0 20 0 27 Data Generate Calibration X Keyword ug ml aenerate Calibration Keyword 2500 2000 1500 Rame 1000 500 0 2000 Calibration Curve BIAevaluation Softwore Handbook ver2 5 55 1 70 0 35 0 16 014 041 0 43 0 51 10 23 0 47 5 56 1 90 0 44 0 01 0 38 0 38 049 014 10 61 0 82 5 52 2 01 0 51 0 07 0 10 0 08 0 04 0 00 0 06 0 01 0 07 0 02 0 01 Y Keyword RelResp Colibration Curve T Keyword Response 4000 0 29 0 23 0 16 031 0 24 0 30 0 18 0 35 0 41 0_22 mn 22 30 ugiml Fitting Function Spline Qubic Spline 5 moothing boundD 5 5 boundD 5 bound 5 bound 5 boundD 5 boundD 5 boundD 5 5 bound 5 bound 5 boundD 5 boundD 5 bound 5 boundD 5 5 bound 5 boundD 5 bound
35. 0 440 Response Time Z Curve Id ks 5 Irm SElrOd C n 0 Offset SE ffset E 22 7 0435498 q 1 b5e 4 4 73 0 0205 1 5e 7 309 37 0 175 0 apr22a bl 8 00233111 1 29 4 3 34 0 0146 1e 7 308 28 2 0 163 apr22a bir 10 1 12 4 1 54 8 13e 3 8 apr22a blr 11 0 0128328 1 32 4 1 26 8 01 3 3 75e 8 1 1 apr22ablr 9 D 0223998 1 03 4 2 55 0 0108 He 1 303 25 9 0 146 1 1 0 0 312 26 4 0 126 0 310 20 0 138 0 Table Define Curve Define Curve X Lurve Mame Cancel lise As x C Index Column Dynamic ly Hew Plot SE UDffset Chi 2 Curve Name X C Y ks OK BIAevaluation Software Handbook ver2 37 2 0 045 0 035 0 025 0 015 2 8 4e 8 5e 8 8e 8 1e 7 1 2e 7 1 4e 7 1 5e 7 0 045 0 0395 0 0295 0 015 26 0 4 8 Be 8 Be 8 1e 7 1 28 7 1 4 1 Be r Linear Fit X Linear fit on selected curves s Fit Apply To Entire Working Set Cancel 38 BIAevaluation Software Handbook ver2 2
36. 00 24500 24000 23500 n 23000 n 22500 22000 21500 21000 1 4 8 Plot 4 B XJ RU 25000 24500 24000 23500 23000 Response 22500 22000 21500 21000 Edit gt Cut 4 BlAevaluation Software Handbook ver2 1 A Plot 1 ni x pus 24900 24600 24400 24200 Response 24000 23800 23600 0 100 200 300 400 500 600 Plot 1 x RU 25000 24000 24600 24400 24200 Response 24000 23900 23600 28 Calculate Y Transform
37. 486 1723 1921 062 Ne 2551 3 beundDSA uk1710 Sample2 assay 256 6 5 0 28036 2 7 02 3 75 0 22 No 1470 9 boundDSA luk1720 Sample2 Sample3 assay 2566 50 273222 1 71 0 90 0 36 INo 790 2 boundDSA luk1720 Sample3 assay 1256 6 5 0 271511 _ 27151 1 1 osa lo 21 1682 3 boundDSA luk1710 Sample1 EIR 5 Data Generate Calibration X Keyword ng ml Y Keyword RelResp OK Cqlibration Curve Generate Calibration x keyword Conc Y Keyword Response C Bubic Spline Line C Paint to Point Fitting Function Spline ly Generate Unk Heplicates Weighting C Median 5moothing Smoothed Calibration Curve 2500 2000 1500 1000 500 e 2000 4000 6000 8000 ug ml View Label Calibration Curve D58 Title DSA OK Y Axis Auto Y Labels Label Cancel Auto X Labels Cancel 58 BlAevaluation Software Handbook ver2 8F amp 2Uv2uvsis cle Unknown HalHtes Calc 45 uk1 1710 6744 1267 075 1h uk1 1 11 674
38. 6 7 5e 7 6e 7 7e 7 8e 7 9e 7 1e 6 conc M CODD BlAevaluation BIAevaluation Software Handbook ver2 41 3 3 1 BlAevaluation Fit Edit Models Models m 1 gt A B AB type 1 type 2 AB type 3 Edit A B AB type 4 1 2 AB1 AB2 tyr 1 2 AB1 AB2 typ 1 2 1 2 AiB Hemove Print Close 111 Description Calculates affinity rom steady state data Heq vs E plot New i Hodel General Fitting Normal Password Formula Info Cancel Independent Variable t Short Description Calculate average response over the selected reagion Constants x 2 Name Dios Fit Keyword Initial Value T average Req EREA 42 BIAevaluation Software Handbook 3 3 2
39. 91778 31914 10 23 0 47 25832 boundDSA 10 000 DSA assay 255 6 50 285400 10 41 5 56 0 38 18728 boundDSA 5 000 DSA assay 255 6 5l 278275 35 1 90 0 38 12038 boundD5A 2 500 05 assay 255 5 50 2 2388 0 93 0 44 049 663 1 boundDSA 1 250 05 assay 255 6 50 268646 013 0 01 0 14 340 5 boundD5A 625 D5A assay 255 6 50 2392053 19 86 10 61 0 82 2734 0 boundDSA 10 000 DSA assay 256 6 51 285453 10 34 5 52 029 Ha 1853 3 boundDSA 5 000 DSA assay 256 6 510 278589 5 3 77 201 D17 12223 boundD5 A 2500 DSA assay 256 6 51 272593 2 098 0 51 10 23 Ha 669 8 boundDSA 31 250 05 assay 255 5 5 0 269029 019 0 07 0 16 366 4 boundDSA 625 D5A lassay 2556 50 26484 4 0 34 010 031 No 75 boundDSA 10 00 196 assay 255 6 50 26430 5 0 26 O08 0 24 71 boundDSAl5 OOO 196 2 lassay 12556 50 264046 0 28 10 04 0 30 INo 62 boundDSA 2 500 196 2 assay 25665 50 263966 016 0 00 0 18 No 1 20 boundDSA 11 250 lg 2 assaw 12566 50 263790 0 33 10 06 0 35 INo 1 45 boundDSA l625 96 2 assay 256 6 50 26361 3 0 36 0 01 0 41 86 boundDSA 110 000 195 2 assay 256 6 5 0 26350 0 0 24 0 07 0 22 No 1 75 boundDSA 5 000 195 2 assaw 1256 6 50 263398 020 10 02 0 22 No 65 boundDSA 2 500 196 2 lassay 256 6 50 26343 2 0 27 0 01 0 30 INo 6 B boundDSA 1 250 196 BM S NNNINNISNININN Edit Delete OK In m In Entire Rows Entir
40. Calculate average response over the selected reagian Fit Cancel Fit Tolerance 0 0005 Apply To Entire Working Set P724 EReAJJU Ft amp 2Uv 2Uss i Curve 19 C Resp Chi 2 GLCHAC Fc 1 GLCHAC Fc GLCHAC Fc 3 Fc 4 Table Define Curve Cancel Dynamic ly Hew Plot C Y Resp Curve Name Ok 46 BIAevaluation Software Handbook 3 ss 1 Curve Ti fms vg G 76 1e 7 22 7 3 7 48 7 6 7 TE 7 7 T 7 Selection XIa2e 7 Y1 42 4510 X2sBa 7 Y2 72 2722 Fit Non linear Fit A B AB type 1 A B AB type 2 A B AB type 3 A B AB type 4 A B1 B2 ABT AB 2 typ Cancel m A B at ES C DI LUE EG Options 3 Drift 3 Offset 1 2 ABT1 AB typ 1 2 1 2 AB Description Calculates affinity from steady state data Req vs plat atsteady state OK
41. D 5 boundD 5 bound 5 boundD 5 bound 5 bound 5 Point to Point Smoothed 000 10 000 5 000 2 500 1 250 625 10 000 5 000 2 500 1 250 625 10 000 5 000 2 500 1 250 625 10 000 5 000 2 500 1 250 625 10 000 5 000 2 500 1 250 Z Generate Unk Heplicates Weighting C7 Median OK 10000 53 19021 0021 89 cle Unknown RelResp Calc ug ml ug ml 11 unkounl 1 2407 7 11820 711 AN ra 7 11820 711 FH A 12 unknunl 122 1654 5 5190 869 ANM 1554 5 5190 8595 ZNH A 13 unkoaun 2 1 1797 7 6024 843 AMO 1797 7 6024 843 14 unkoun 2 122 1110 7 28554 158 AM 1110 7 2054 158 N A 15 unkoun3_1 2117 3 8455 925 AMO 2117 3 8155 925 16 unkoun3 142 141 4 9 4029 425 AN 1414 9 41029 47 ENA 4 K UVZNOSXEIX Calcug mlSg csmetiesd cf 707 7 U1 5 RelResp AVG CV CV 4 3
42. GE Healthcare 3 Biacore BlAevaluation Ver 2 1 Instrument Handbook EX Fa 1 Die eem cere E GE imagination at work BlAevaluation Software Handbook BlAevaluation Biacore Handbook BIAevaluation Software Handbook 10 3 10 11M kalka 104 2x1012M Ko 103 107M 1s 1 EZ 10 101 1 1 1 2 pes ss 1 4 Q S D
43. alibration C C cv ASSAY3BLEB ASSAY 3 eun 5000 4000 3000 2000 1000 0 2000 4000 Bun 10000 12000 2 BIAevaluation Software Handbook rO ERROA LU EIB PE OREORE A coUv duUlcla 151 89 ms sess CES gt _ T E vm i IC 525 576 D DR gt on gt m
44. b2micro b2micro 3 67097 2 19450 0 50832 second Ab hgmicrn 0 33230 0 06165 0 33329 baseline 1 1 2 2 2 3 3 3 4 4 4 5 5 5 b b b 7 T T 8 8 8 g Fc Id BASELINE Data Extract Keyword Keyword BEC QUXd SO Keyword Fc Value Text 2 OK Value Text 2 El ly Mew Table Cancel Datqa Extract Keyword Id Value Text bound OK 52 BIAevaluation Software Handbook A BIA Concentration Evaluation SSA Y3 BLRx boundDS 3 File Edit Data Window Help Cycle APROG Time window AbsHesp SD Slope LRSD Baseline RelResp ld ngyml sample 10 01 0 45 29020 89 28507
45. bels Plot 11 Imp xj RU Protein Interaction Frntgin S0uginl Pratein amp 25udml Response Cc c 150 Pratein 12 5 40 20 20 40 B at 100 120 140 160 Time 16 BIAevaluation Software Handbook 1 View gt Legend OK OK Hidden Left Top Right Bottom 60 120 180 240 300 360 420 480 540 600 Project d Kinetics Fc 4 kinetics ble Kinetics Fc 4 kinetics ble Kinetics Fc 4 kinetics ble Kinetics Fc 4 kinetics ble PEE Kinetics Fc 4 5 kinetics ble BIAevaluation Software Handbook ver2 17 1 Curve Prop
46. e Column s Cancel A BIA Concentration Evaluation 4SSAY3 BLR x File Edit Data Window Help aar O OND a Q N assay 256 6 8 18 74 1 0 01 0 45 usa 2436 0 boundDSA 1 0 000 DSA assay 255 6 2850726 10 38 5 55 014 No 1887 9 5 000 DSA assay 255 6 5 0 277825 320 170 041 1158 4 boundDSA 2 500 DSA assay 255 6 5 0 27239 7 0 76 0 35 0 43 No 652 7 boundDSA 1 250 DSA assay 255 6 5 0 26883 2 0 55 0 16 0 51 No 346 3 boundDSA 625 DSA assay 255 6 5 0 29177 8 19 14 10 23 0 47 No 2683 2 boundDSA 10 000 DSA assay 2556 5 0 28540 0 310 41 5 56 0 38 1872 8 boundDSA 5 000 DSA assay 2556 5 0 27827 6 3 57 1 90 0 38 1203 8 boundDSA 2 500 DSA assay 255 6 5 0 27238 8 0 93 0 44 0 49 663 1 boundDSA 1 250 DSA assay 255 6 5 0 268646 013 0 01 0 14 340 5 boundDSA 625 DSA assay 255 6 5 0 29205 3 19 86 10 61 0 82 2734 0 boundDSA 10 000 DSA assay 2566 5 0 285463 10 34 5 52 029 1869 3 boundDSA 5 000 DSA assay 256 6 5 0 278586 3 77 2 01 0 17 No 12223 boundDSA 2 500 DSA assay 256 6 272592 d s 23 669 8 boundDSA 1 250 DSA assay 255 6 26902 9 366 4 boundDSA 625 DSA 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 N assay 2566 50 279478 EE INI N NI ISNI BIAevaluation Softwore Handbook ver2 say 256650 228 975 03B 1624 No B744 boundDSA eki10 Sample1 2 assay 256 6 5 0 26635 6 0 34 0 14 0 25 INo 3211 boundDSA luk1720 Sample1 assay 2566 s0 288
47. erties Name OK Properties 1 Prnotein 5 Frotein A Protein A dLluggml Prntain A 20ug ml Frotein A 10ug ml 18 BlAevaluation Software Handbook ver2 1 Curve Properties Color M Lish nea OK urve Properties c L wawa ww m E Marker Flot 1 2m i E o x 700 BU 500 400 300 Protein A ugrml h BIAevaluation Software Handbook ver2 19 1 View gt Scale Auto X Y OKk Plot 11 RU ProteinA Interaction 450 400 350 300 250 Pr
48. otein 5HudMTI 200 Protein 25ugitml 150 100 Response Protein 12 Sucml 50 Time 20 BIAevaluation Softwore Handbook ver2 2 2 2 1 Non Linear Fitting Apr22 blr amp APR 2 BLE n ld 5uource 22 7 1 APR22 BLE 22 8 1 22 BLE 22 9 1 22 BLE 22 10 1 22 BLE 22 11 1 22 BLE 22 32 APHR22 BLE 22 33 APR22 BLE 22 34 APR22 BLE 22 35 APR22 BLE 5 Plot 1 _ X 22000 Response 17000 12000 200 400 200 1000 1200 1400 1600 2 BIAevaluation Software Handbook ver2 21 2 Plot 1 x RU 32000 27000 22000 Response 17000 12000 0 200 400 Bu ang 1000 1200 1400 1600 Edit Cut Calculate gt Y Transform De Plot 1 xl RU 160 140 120 100 al rie B Response 40 20 M 0
49. valuation Software Handbook ver2 1 Plot 1 al x 15000 14500 14000 13500 13000 I 12500 12000 0 s 1000 1500 2000 l Plot 1 E x 15000 13500 zi ii 13000 12500 L 12000 4 8 D 500 14500 14000 Edit G Graph 1000 1500 Edit Paste Curve Paste x n Original Position gt 5S pecilied T gt amp TY w ll Paste At xo X AL w Specified X amp Y OK Cancel BlAevaluation Software Handbook ver2 11 1 l4 Plot 1 n X 1500 1000 X Y 0 File Close Window gt Project 1
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