CytoFLEX フローサイトメーターでの DuraClone 試薬の測定：CytoFLEX
Contents
1. DuraClone Dry Dry CytoFLEX A PROTOCOL DuraClone IM kit2. Acq Setting Grab amp Drop Cytometer Acq Settings Gain TSet as default CytoFLEX IFU V 4
3. CytoFLEX 405 nm 80 mW 488 nm 50 mW 638 nm 50 mW 18 DuraClone IM kit 488 Excitation 638 Excitation 405 Excitation 1 a 8 A700 Pacific Krome A647 9 Blue Orange A700 B53309 NS A Se CD16 CDse CDig CD14 EE CD45 DuraClone IM B cell Tube B53318 RUO 25 tests cpa1 CD19 CD27 CD24 i CD38 cp45 DuraClone IM T cell subsets Tube B53328 RUO 25 tests CD45RA CCR7 CD28 a CD27 CD57 CD45 B53351 Dura Clone IM Dendritic cell Tube CD16 Lineage CD1e cD11c Clec9A HLA DR CD45 RUO 25 tests CD123 B53340 DUAGIONG I MGS MD TCRgd TcRab HLA DR rcnvaiLcp4 cps 0 RUO 25 tests s CD3 CD19 CD20 CD14 CD56 1 Alexa Fluor 700 2 APC Alexa Fluor 700 3 APC Alexa Fluor 750 4 Alexa Fluor 647 2015 9 CytoFLEX Xitogen Technologies
4. Label 4 d Acq Setting Acq Setting 4 Acqg Setting Gain QC Recommended 5 d CytExpert FSCSSC 5 VersaComp beads
5. Auto 5 6 7 6 Compensation Calculation Settings QC Advanced Help Compensation Setup Compensation Matrix Events Display Setting Options 2015 9 7 1 Save as DuraClone xxx Lot yyy Save to Compensation Library DuraClone xxx Lot yyy File 5 New Expe
6. Dry 1 1 WEB http www DuraClone com im CytoFLEX Daily QC Fluorosphere VersaLyse Lysing Solution IOTest 3 Fixative Solution VersaCOImD Antibody Capture Kit 1 DuraClone IM kit IFU 2 DuraClone Compensation Kit VersaComp beads 5 a VersaComp Antibody Capture Negative Bead VersaComp Antibody Capture Positive Bead 1 DuraClone IM Kit Compensation Kit b 20 3092C 15 c 1 mL VersaComp beads VersaComp IFU 300 xg 6 f
7. DuraClone IM Kit DuraClone IM kit Dry RR AT CytoFLEX REFERENCES 1 CytoFLEX B49006AB 2 Weit N Kapinsky M amp BOhmler A 2015 Advanced analysis of human T cell subsets on the CytoFLEX flow cytometer using a 13 color tube Dased on DuraClone dry reagent technologyr Beckman COu fer Application Information Bulletin FLOW 817APP03 15 A NOTES 488 nm 638 nm 405 nm CytoFLEX
8. Alexa FIuor Pacific Blue Molecular Probes gt BECKMAN COULTER 135 0063 3 5 7 TOC 0120 566 730 http 7www beckmancoulter co jp 2015 9
9. 2015 9 File gt Save as template DuraClone xxx xxx IM T Cell 12 New Experiment Ctrl N New Expenment from Template New Compensation Open Experiment Ctr O Open Compensation Save Ctrl S Save As SaveAsTemplsten Import FCS File Export FCS File Recent Recent Template Recent Compensation Close Experiment Eat 12 1 INew Experiment from Template 13 DuraClone xxx Experiment DuraClone xxx Acq Settings Gain a Recommended QC New Experiment Ctrl N _ New Experiment from Template New Compensation Open Experiment Cri O Open Compensatbon Save Crl S Save As Save As Template Import FCS File Export FC
10. 600 uL BE COULTER Life Sciences CytoFLEX 1 CytoFLEX 2 CytoFLEX 3 CYTOFLEX DuraClone IM kit 1 File New Compensation 1 2 DuraClone xxx Lot yyy xxx IM T Cell yyy DuraClone IM kit 2 y ATC 3 File Cytometer Settings QC New Experiment Advanced Ctrl N New Experiment from Template MO Open Experiment Open Compensaton Recent Recent Template Recent Compensation Exit 1 Ctrl O QC Compensation Setup 3
11. CytoFLEX DuraClone CytoFLEX Dr Nicole Weit Dr Andreas Bohmler Dr Michael Kapinsky 1 2 S A S SUMMARY PMT CytoFLEX 1 INTIRODUCTION
12. S File Recent Recent Template Recent Compensaton Close Experiment Ext 13 b IDefault a 14 Settings QC Advanced Help Set Channel Set Label i Compensaton Mer Compensation Library Events Display Setting Options 14 8 b Import from Library 59 2 15 DuraClone xxx Lot yyy OK 16 vcle16Z lt 16 IImport compensation matrix and transform with current gain OK 17 Import Compensaton 9 Import compensaton matr and transform it with current gains import compensaton matro Import compensation matrix and gain 17 Experiment Tube Label 2015 9 RESULTS CytoFLEX
13. riment DuraClone xxx xxx IM T Cell Settings Set Label 8 Set Labc Channe Labe Frc CD45SRA FITC PE CCR7 PE ECD CD28 ECD PCS 5 CD279 PCS 5 PC7 CD27 PC7 APC CD3 APC APC A700 CDS AF700 APC A750 CD3 APCA750 PB450 CD57 Pacblue KO525 CD45 KrO Violet610 CD95 BV605 Violet660 CD2S BV 650 Violet780 CD127 BV785 Apply to B Current Tube ubes Cance 8 4 Settings 9 Compensation Matrix Import 10 Settngs QC Advanced Help Set Channel DuraClone Set Label com i 11 Compensation Library Events Display Setting Options 9
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