フェアー - 和研薬
Contents
1. 196 190x250mm 7mm 250bp 23kb 0 75 29 a I CE 2 1000g cm 196 70mA 4 2000g cm 1 59 lS A noel 72 36C 1 5C 3 Easy Ladder 88C 1 5 Bioline Cat No BIO 33045 lt 0 4596 4 Tkb DNA Ladder RTU 0 196 NipponGenetics 4 NTU Europe Cat No NE MWD1 DIR Cat No NE AG01 Cat No NE AG02 9 600 wo5 000 FastGene a ini Kit P58 LB LB 40ml 10 400ml 5 1000mL LB2. lon Torrent PGM Pippin Prep Read Length 50bp Pippin Prep kk 2013 2013 10 1 12 31 info genetics n co jp I 2013 9 1 W WEB
3. 100bp DNA 200bp DNA HIiSeq1000 2000 MiSeq KAPA 1 KAPA real time library amplification kit PCR enrichment over amplification over amplification DNA PCRH SybrGreen PCRH PCH KAPA HiFi DNA polymerase
4. Cat No CS 06 001 600 i 5 11 i 80C 80C Direct 2 ee TTS Direct PBS Direct Direct Direct kg 0 80C 2013
5. 0C 100 C 4 9 RHS238 0 1C 0 2C Cat No MC 01N 110 SIB9iD00 Cool Hotter PCH MC MP01 MC MP02 MC B0 5 1 5 MC B02 05 1 5 MC B0 5 MC B1 5 15 000 22 000 18 000 18 000 18 000 18 000 MC B17 MC B29 MC B1 5H MC B0 5H MC B0 2H 18 000 18 000 11 000 11 000 11 000 Personal DEEP FREEZER
6. 1 5 0mL 0mL 64 Si eee Scientificrurc 8 80C 121C 3 10x1258x33 5mm LXWXH 133X133X53mm LXW XH Pile Cat No HS120018 IAN 1 SI Ye 700 0 1 5 2 0ml lt 1 5 2 0mL 50 Heothrow Scienkificruuc New Ceew same
7. 56 7cm2 100 x20mm 100 mm 90 mm AI D R Ne Free Er Pyrogenic Cat No FG 2090 240 10 X24 HPLC ee HPLC Cat No SLNY1322N 0 22um 13mm 100 SLNY1345N 0 45 m 13mm 100 SLNY2522N 0 22Lum 5mm 100 SLNY2545N 0 45 m 55mm 100 PTFE Cat No SLPT1322NB 0 22 m 13mm 100 SLPT1345NB 0 45 m 13mm 100 sii ls SLPT2545NB 045um 85mme 1OO PTFE
8. 1 5 2 0ml X12 500 12 500rpm 0 2ml 8 4 or 3 EA 0 2m x12 203x171x114mmtWDxtD 9 800Xg 0 5ml 12 100 240 VAC 50 60 Hz NOUSU 4 29 000 I HS100502 9 200 HS100503 16 06 18 000 HS100504 8 16 000 Cat No HS10050 HS100505 2 6 200 99 900 HS100506 1 5ml 7 900 2013 2013 10 1 12 31 info genetics n co jp NN Ee Cool Hotter OC 100
9. 340x280x480mm WxDxH 5 6kg 2GB 12M PC USB2 0 UV 340x280x320mm WxDxH 2 0kg FAS Digi FAS Digi Cat No FAS DGMU Cat No FAS DGDC Cat No LB 16K 470nm 100 5026 200x160mm 185 000 67 000 98 000 340x280x80mm WxDxH 96 CatLNo MB 16K UV 302nm 98 000 200x160mm 340x280x80mm WxDxH 4 3kg FAS Digi 350 000 UV 8W UV X5 Cat No AP 03M AT 95SG AP 03M 6 15 900 FAS 2530M OG FC FG 830
10. Info genetics n CO jp 2013 2013 10 1 12 31 infoQgenetics n co jp Application Note 2012 lt 11 ee jbidi Pump System iDbidi EE Ibidi IDidIi HUVEC perfusion HUVEC perfusion vivo in vivo ibidi iDpidl
11. Cat No BMF7510 BUF7510 BLF7510 BDF1510 BDF2010 ioo BDF3010 90 Q age science 90bp 27kb 30 8 10ug 9DNA 2ug DNA PCR OO 2AOV Eonstany LN 5900Ag 470nm 525nm 280X530X180mm WxDxH kg 0 100 200 300 400 500 600 700 60090010002 4 6 8 10 12 14 16 18 20 22 24 26 28 bp kp DNA InfoQ genetics n CO JD DNA Pippin DNA PR NE DNA 2 DNA Ck 1 Pippin Prep
12. 1 Es y sa N eNll Cat No 3 TR5000 9 5cm 6well 50 TR5001 3 8cm 12well 50 TR5002 1 9cmS 4well 50 TR5003 0 32cm 96well 50 28 2013 2013 10 1 12 31 info genetics n co p 15ml 8 000XG 5Oml 10 000XG 10 oe Jp RR 4 Free Free Pyrogenic 15ml Cat No TR2000 50 x10 500 8 900 17 8 TR2001 50 X10 500 8 000 16 0 TR2002 x 125 x10 1250 _ 16 600 13 3 Cat No TR2003 25 X20
13. LXxW Well clearance Bottom info genetics n co jp 175um kT 56 60C 127 7x85 5x22 5 20 5 mm 18 5 mm 19 0 mm 15 5 mm 1 90 cm2 4 30 cm 1000u 1000 Hl 10 um 5 MW 175um 7 56 60C X06 5X 1 62 15 6 jm 13 0 mm 9 1 mm 7 3x7 3 mm 0 55 cm 2 35 cm 300u 300 LI 5um 25 um 96 127 7x85 5 mm 16 6 14 4 mm 9 0 mm 10 ul 4 mm LOL 5 mm 0 125 cm 0 23 cm 1 0 mm ibidi Standard Bottom 180 um 2 a Slide 2well 1500 ll a 4 8cm a jbiTreat Yl EOG Lu Slide 2 well Lu Slide 2 well Ph Ca
14. KAPA 0 U U 6 0 0 5 2012 7 27 LIMprep 6013 4 Genome Biology KAPA Library Preparation Kit 3 10ng DNA n 9 lllumina MISeq DNA 10ng DNA CovarisS S220 100bp
15. MG MG SYBR Green 1 Il SYBR gold SafeView 10 150V 1V 10 300mA 1mA Output Voltage Inc Output Current Inc Max Power 30 watts Cat No MBE 150PLUS MBE 150 M A US 6 S a N _ LED 470nm LED 9000 40C 6 lt NERD 293x200x80mm WxDxH Cat No RT MBE 150 ES 158 000 0 MBE TLC 86 000 GL A i sim Input 100 240VAC 50 60Hz 1 4A Mupld 2plus Output 15VDC 4 0A 60W max MAASL Sx4 Lx2 4 800 Mupid exU OO X2 X1 I 1 MBE TANK
16. Cat No ib82401 60 000 60 000 u Plate 96well IDiTreat iDidi elei i Cat No ib89626 15 ibiTreat 15 60 000 60 000 Cat No Iib89621 gt EY Plate Ang genesis 96well SBS
17. 2013 2013 10 1 12 31 info genetics n co jp 6 HUVEC perfusion sprouting assay HUVEC ibidi 5dyne cm5 sprouting HUVEC ibidi ibidi EGM 2 BD X70 Hoper Image Pro Plus HUVEC iDidi HUVEC
18. 105x200x23mm LXWxH Cat No HS4283P Cat No HS23229B aE 10 4 ii WDB00 SL23600 A i a 2013 8 7 ttD377WWWii deTetICSiCOm 40 2013 _ 2013 10 1 12 31 info Qgenetics n coJp New 1 2 0ml ecoy Scientificuc 100 141xX151xX57mm LxXWxH I 45 Cat No HS120042 Wl Cat No HS120043 Wl Cat No HS120044 i 5 5 5 1 5 980 5 980
19. 2013 10 9 http www n genetics com 113 0033 6 17 9 3F Tel 03 3813 0961 Fax 03 3813 0962 604 8277 565 F Tel 075 257 5421 Fax 075 257 5422 TONT309A39K FAQ
20. Cat No GSPG20 1 Cat No GSPG trial ES iP 5 000 43 000 1mm 1 1 A solution B solution 20 in situ 0 A B 9 EE k A B In situ Hybridization R _ es In situ
21. 19 6 ww 75bp 5kb Cat No KK3606 2 5ml 200 0 000 25ml 2000 25 1 500 KAPA2G Robust HotStart ReadyMix with dye ee KAPABIOSYSTE 18 5 tubDe PCH KO allele 1 PCR FKAPABIOSYSTEMS
22. Universal qPCR ABI Prism qPCR ROX Low Bio Rad ABI7Z500 ABI qPCR iCycler qPCR Light Cycler ROX ABIZ500 ViA MX3000P hi 0 lt iCycler iQ iQ 5 MyiQ ME MX3005P MX4000 Cat No Kit contents KK4700 KK4704 F KK4708 30 20ul 5 300 2013 2013 10 1 12 31 info genetics n co jp KAPA LiDrary Amplification Kits LA LA IFKAPABIOSYSTEMS WM PCR KAPA HiFi Hot Start
23. Vent 0 2 m PTFE Pyrogenic 25cm 5 7ml Cat No TR6000 Vent 10 X20 S00 TR6001 NON Vent 0 X25 500 75cmS 15 22 5ml Cat No TR6002 Vent 5 xX20 100 TR6003 NON Vent 5 X20 100 162cm2 3 48ml Cat No TR6004 Vent 5 X5 TR6005 NON Vent 5 X5 Y Do HE3D 1 Pyrogenic Cat No X TR4000 8cm 35x10mm 0 X25 500 TR4001 1 cm 60xX15mm 0 X25 500 TR4002 55cm 100x20mm 20 x25 500 TR4003 148cm_ 150x25mm 5 x12 60
24. molecular ibidi PumpSysfem HUVEC endothelial cel IDidi 1 2013 2013 10 1 12 31 info genetics n co jp HUVEC ibidi Slidel Luer0 6 C0e
25. a 4 4 1mg mL 2mg 3 6 2013 2013 10 1 12 31 info genetics n co jp GenoStaff um enosta IPGell IPGell
26. info genetics n CO jD 2013 2013 10 1 12 31 info genetics n co jp Ga 4 USB FAS IV 5 USB USB FAS Digl PC Tr of FAS LED BOX PC 1 FAS Digi 0 5
27. 10 Cat No ib89646 15 iDiTreat 96 lt pO a 50l ww Cs AA ea 30 2013 2013 10 1 12 31 LxWxH lt LxWxH lt
28. 80C ES OP9 SNL MEF OKT4 K562 HEK2993 HEK 93T HeLa CoC12 PC12 HepG2 PCR DMSO 10C
29. 0 2mlX96 0 2ml 96 0 1 4C 0 2ml 8 X 12 9 0 2ml 4 105C 4C sec 4C sec TS 399 000 0 2C 55C 60 LOAC 595C 1 30C 250 99 PCR G eC elo U C h ON 5 Cat No TC EA 9
30. C57BL 6 i 14 5 14 5 14 5 I E 16 5 16 5 16 5 16 5 16 5 16 5 16 5 18 5 18 5 18 5 18 5 18 5 18 5 18 5 18 5 EN Ei 1 1 Bb I T TE Emp 11b Emp 10k 18 5 Emp 1 1c 8 Emp 11k Emp 11d Total RNA Panel 10 ug Cat No GSGMTP 10 Cat No GSMEP 8 35 000 50 000 70 000 Panel 1st strand cDNA Panel C57BL6 8 Cat No GSMDP Brain 8 Panel NC NgaSlc Cat
31. bp ng 0 5ug bp ng 0 25ug 20 4 54I 1mm 20 4 8000 31 25 12 5 20 4 20 4 7 20C 1 4C 6 60 12 49 12082 43 5 20 4 100bp 10kbp 18 100ng l a 1600 62 40 Cat No KK6302 Cat No KK6303 200 1000 200 x5 20 60 20 20 100bp BkbpD 7 7 60 50ng 9 700 83 000 800 400 20 20 20 20 20 Cat No KK6304 Cat No KK6305 A 000m G00 0700 YY 33 000 200 100 1 agarose in 1X TAE 196 agarose in 1X TAE HyperLadder eeue DNA HyperLadder 1 1kb HyperLadder IV 100bp bp ng Bang bp a 1 om 10 1 HN
32. Cat No KK2601 5 1kb GO 6596 650bp 1 25ml 100 H 25I 9096 8796 GC rich 9 000 UE UEO CE 30 DNA 6 25ml 500 25 RED00 ck Sx 2013 2013 10 1 12 31 info genetics n co jp
33. EY PiDDinPulse DNA 12X14cm 3 Cat No PPI 0200 700 000 10X KBB buffer for the Pippin Pulse Cat No KBB1001 1L 40 000 A SN 200mA Oe mn PD 1 1kg ELD 1 14 Gm Lonza SeaKem GOLD 1 10X KBB buffer pH8 7 fo
34. ISH ISH Google Scholar genostaff 100 ceroStat 900 000 ELISA lst Snd 1 1
35. 3GB 950 000 8 USB 4 TIFF JPEG BMP PNG a gt 35x31x48cm WxDxH 8 Fe LED ON OFF USB 11 2kg UV OMOFF UV High Low We LED UV 312nm UV 21 X21cm 21cmx 19cm UV 8WXx6 9 Exposure Time UV High 100 Low 70 Date OS sm sm 100 240V 0 8A ilslalrliielrlelrls mlerlmlelelmlslElli mw mr FAS IV
36. ibidli 10dyne cm 5 10 HUVEC iDidi iDidi u Slidel Luer0 6 iDiTreat iD80186 iDidi EGM 2 DAPI PE phalloidin F actin IX70 Roper Image Pro Plus HUVEC iDbidi HUVEC static Condition 10 a 2 scale 500um hm
37. m The Zinc Transporter SLC39A13 ZIP13 ls Required for Connective Tissue Development Its Involvement in BMP TGF B Signaling Pathways PLOS ONE3 11 e3642 2008 Toshiyuki Fukada Natacha Civic Tatsuya Furuichi Shinji Shimoda Kenji Mishima Hiroyuki Higashiyama Yayoi Idaira Yoshinobu Asada Hiroshi Kitamura Satoru Yamasaki Shintaro Hojyo Manabu Nakayama Osamu Ohara Haruhiko Koseki Heloisa G dos Santos Luisa Bonafe Russia Ha Vinh Andreas Zankl Sheila Unger Marius E Kraenzlin Jacques S Beckmann Ichiro Saito Carlo Rivolta Shiro Ikegawa Andrea Superti Furga Toshio Hirano 1 Koizumi S Shigemoto Mogami Y Nasu Tada K Shinozaki Y 0hsawa K Tsuda M Joshi BV Jacobson KA Kohsaka S Inoue K Nature 2007 Apr 26 446 7139 1091 5 T Nakamura M Nakagawa T Ichisaka A Shiota and S Yamanaka Mol Cell Biol 31 21 4366 4378 2011 3 H Fujishiro Y Yano Y Takada M Tanihara and S Himeno Metallomics 4 7 700 708 2012 4 S Hayano H Kurosaka T Yanagita Kalus F Milz Y Ishihara Md N Islam N Kawanabe M Saito H Kamioka T Adachi T Dierks and T Yamashiro J Biol Chem 287 15 12217 12229 2012 Google Scholar genostaff 100
38. Cat No TC 96GHbC 299 000 100 GeneQ 2 24 Bioer Technology Cat No TC 24Hb 250 000 100 4C 0 1C 0 2mlX96 96 PCR 0 2ml x96 8 12 gt 105C 4C sec 4C sec sl lt 0 4C gt 95C 1 30C 250 99 PCR 0 2mlx24
39. PC 2 FAS LED BOX W198xD254xH227mm 900 dBM CMOS CMOS DNA LED 2 AGtWMcorm Green dala il a 4 IMIOOTIGTe f JPEG AA SD SDHC is 16X11 5cm Cat No FAS BOX 220 000 LED LED LED 520nm Amber LED
40. GenoSfa in situ Cat No GSSRK 01 15mL 0 10x 500mL G Block 15mLx 4 2013 2013 10 1 12 31 info genetics n co jp Total RNA amp 1st Strand cDNA PCR 40 22c cy 1st strand cDNA Panel lst strand CDNA Panel C57BL 6 8 C57BL 6 Whole RNA olIololeololololo O O oN on rR wm CIEIEIEIEI 10 8
41. DNA PippinPrep 330Dbp PippinPrep Sample3 1 Agilent Technologies Bioanalyzer sample 1 L 330Dp sample 2 L 330Dp 1 1 4 Im I OO Hm EE el im Fm il Fe ban a sample 3 PippinPrep 315Dbp 1 a TT T T F i ai lf Wi Fm FE Tm Fm a a sample1
42. ELISA ELISA lst 24 well 2 1 ug mL 1 mL ex etc 10 3 SA et 2nd
43. 0C 1 196 75bp 5kb Cat No KK5706 5 ea 2 5ml 200 25u 14 500 Cat No KK5707 25ml 2000 7 25 ul 114 500 PCR II 5 UNG 2 9 55C 1 DECET
44. UV LED 490nm EZ MM 530nm gOnm EE3g 4C 1 dsa a a 3mM 100ml pe 4 6 L 0 3M NaCI 0 1ml Am eT ee EO 19900 100ml Advance 0 II 2 3 560 0 5cm 5 45
45. LED SDS PAGE CBB i LED DNA SBC BSNS SD a PC DR EY FAS LED BOX W188XD254XH287mm 900 dM CMOS CMOS 2 DINIOA 02 8 OM 470nm 2 2SdeS MidOorIGreen
46. wy 218 900 12 000 8 500 3 980 50 FAS 2530M FG 830 P18 0G FC 2013 2013 10 1 12 31 infoQ genetics n co jp RMAA NIPPON GCS Genetics carua CCD UV USB PC UV e SYBR Green e CCD Type 1 3 USB 4 2 2 75 1024x768 eo gt 3 lt LED 020 i 52mm 10 4 Cat No FAS4
47. PippinPrep sample3 sample4 DNA sample1 sample 2013 2013 10 1 12 31 info genetics n co jp Read Length Histogram sample 1 L 330Dp sample 2 L 330Dbp Tien dais oo Reit Le rtegtiat Total Number of Bases Mbp 348 76 _Total Number of Bases Mbp 148 08 Number of Q20 Bases Mbp 228 38 Number of Q20 Bases Mbp 100 36 Total Number of Reads 2 898 964 Total Number of Reads 1 577 037 Mean Length bp Mean Length bp Longest Read bp Longest Read bp sample 3 PippinPrep 315bp sample 4 PippinPreD 375bp ne mi i mn 3 Jr nen Total Number of Bases Mbp 932 52 Total Number of Bases Mbp 550 06 Number of Q20 Bases Mbp _ 702 58 Number of Q20 Bases Mbp 406 80 Total Number of Reads 5 302 371 Total Number of Reads 2 668 599 Mean Length bp Mean Length bp Longest Read bp Longest Read bp Ya Life Technologies lon Torrent PGM Read Length
48. Standardization Tip Cat No 15671T _ L a 1 1 200l 4 it 2 96 x50 yy23 000 MulTT Fit Tip EZ000 2013 2013 10 1 12 31 infoQgenetics n cojD 23 gl DIRI Free Free MA 0 2ml PCR Cat No FG 021D Cat No FG 021F 0 Oo my 2300 2300 0 2ml 8 PCR EE IE TE Cat No FG 0028DC SE Cat No FG 028DC 120 12 X10 Cat No FG 028FC Cat No FG 0028FC SE 12 x10 120 24 2013 2013 10 1 12 31 info genetics n co jp
49. DN DC 6 16x11 5cm FAS BOX 220 000 LED LED LED 520nm Amber LED LED LED 2 7 m0 B USB PC mm 100 240V 3A 198 x254x227mm WxDxH 3 2kg EE a Midori Green Direct SDS PAGE CBB 2013 2013 10 1 12 31 info Qgenetics n cojjp 7 s Y major scClehnee 4 SYBR Green 1I 15OV _ DNA RNA LED w SafeBlue System LED amp
50. LED DNA 5 2 2 2013 2013 10 1 12 31 info genetics n co jp Fast Gane SN Midori Green Direct
51. RCA 2 FAS Digi FAS LED BOX SD PC SD PC PC 3 FAS Digi PictBridge USB PictBridge egllelo ZN USB PictBridge http www ci
52. eta as 2013 2013 10 1 12 31 info Qgenetics n co jp 3 7 NIPPON Genetics EY Genetics cL 12 LED FUT NA P4 5 5 CaBI LED DNA OU 2 We FAS Digi FAS 340xX280x480mm WxDxH 5 6kg 2GB 12M PC USB2 0 UV 340xX280x320mm WxDxH 2 0kg FAS Digi FAS Digi cat No FAS DGMU cat
53. GC AT a i I Wellcome Trust Sanger NGS 300 Michael A Quail et al Optimal enzymes for amplifying SeduenCing libraries Nature Methods 9 10 11 2012 L 3 lt 2 2013 2013 10 1 12 31 info genetics n co jp LIMprep Workflow lt Start material gt 10ng fragmented mouse genome DNA n 9 lt KAPA library preparation kit gt End repair dA tail adaptor ligation lt KAPA real time library amplification kit gt Determination of PCR cycle lt KAPA library amplification kit gt PCR enrichment RS Library DNA gt Quantified by pico green Average 73 8 4 8 ng CV 0 066 Mi by qPCR gt 1ng Library DNA gene locus n 9 Detected 8 gene locus A H ieee 4 lt KAPA Iibrary quantification kit gt Final QC Eh mm qPCR lt D
54. 92mm x 30mm 2 Cat No HS23051 1 HS23053A Cat No HS23053A 5 lt Hesthron same amp 215x300mm 1 5mil 91X128xX14mm Cat No HS1003D 1000 PCR 0 mIPCH 130X88X33mm 446 9 Cat No HS23461C 3A 5 lt Cat No HS23461A ETAN 1 1 5 2 0ml Geewoy Scientificuc 1 5 2 0ml Cat No HS2165C ZA 5 2013 _ 2013 10 1 12 31 infoQgenetics n co jD 45
55. 5 640X480 262k 2 LifeEC o BIOer 5 Technology USB USB 96 5 0 1 4C POST Power On Self Test
56. e00000000606 6 e0000000006 6 e000 oo ee e00000000606 6 oeo e e eeeeeee ee 4 o o e e eeo e e e e00000000606 6 Cat No 4Ti 0563 5 GO Scientificrurc 1 5ml 2 0ml 80C 1 5ml 2 0ml 100 14 1X 15 1X 5 7cm Cat No HS1065 100well 1 lt 100 sl P41 0 Q Heathrow Scientific urc 1 5ml 2 0ml 80 1 5ml 2 0ml 0ml Cat No HS29025G Heathrow Scientific ic 0 5ml 1 5ml 2 0ml 0 5ml x96
57. Cat No ib80209 25 Dish Culture Insert u Dish35mm High Dish Cat No Iib81176 ibiTreat Culture Insert 2013 2013 10 1 12 31 info genetics n co jp 31 1 2sm a Pyrogenic 1 TR3000 1ml 0 3ml 71mm 1 3 1 8mm 500 6 500 13 0 TR3001 ml 0 4ml 71mm 1 8 2 2mm 500 6 500 13 0 TRS3002 5ml 3ml S894mm 1 4 2 0mm 50 5 700 22 8 TR3003 10ml 3ml 594mm 1 6 4mm 00 4 580 22 9 TR3004 25ml 2 6ml 297mm 2 3 3 5mm 100 4 800 48 0 R U 0 0O eeex Scientific uc
58. 2 2cm jbiTreat ele Lu Slide 4 well Lu Slide 4 well Ph Cat No ib80426 Cat No ib80446 15 15 Eo micro Insert 4Well es PIG micro Insert 4well 4 10 10 0 03cms x4 jbiTreat 4 Dish micro Insert 4well uu Dish35m High Dish Cat No iDb80406 30 ss i Culture InSeLt W Did gt wound healing 70ul 0 22cm 2x2 Culture Insert
59. 8 3 D Cat No NE MWP03 Cat No NE MWP03 8 100 600ub 800 00ulIX8 9900 74 000 1 5 TT 2018 9 ue A A Ae laa ey 1 2 5 PA 2 A 2 7 gt A we uu _ 2013 2013 10 1 12 31 info Qgenetics n co jp SOrenson 4 BioScience Inc Er WM One Touch Y D R P A Cat No 10320 N A 960 CE EE 45mm 33 ee gt m 964x0 22 Cat No 10380 50 1250pl mo64z6 Goamm ama 5500 HT
60. LB 1 Q Elg 2 15 3 FF Cat No NE L2720 100 Q 3 40ml 100 PP 5 900 1000ml 2013 2013 10 1 12 31 info genetics n co jp FastGene DNA a Bess Palrs 1 PPE 100 g ml L000 ia SFA z up HY zoo 3EE3g 25C 1 G0C m 100bp DNA Ladder lkb DNA Ladder 100bp 3kb S50bp 10kb 12 13 Cat No NE MWD100 Cat No NE MWD1 3 900 yg 900 1 5 TAE Marode Bi 1 3 TAE agarose gel 8 K A DA N A eKAPABIOSYSTEMS DNA
61. 89130 886 Blue 200 6 500 32 5 89130 888 10ml 0 1ml Orange 00 6 500 32 5 89130 890 5ml 0 2ml Red S00 15 000 75 0 89130 896 Blue S00 6 400 32 0 89130 898 10ml 0 1ml 0Crange S00 7 200 36 0 89130 900 ml 0 25ml Red S00 15 000 75 0 89130 902 50ml 1 0ml Purple 100 25 000 250 32 2013 2013 10 1 12 31 info genetics n co jp 15ml 8 000XG 5 10 000 x G 10 4 D R KON lL 2 ES CS a EE 15ml Cat No TR2000 50 x10 500 TR2001 50 X10 500 TR2002 x 125 x10 1250 Cat No TR2003 5 500 TR2004 50 10 500 TR2005 X 60 10 600 NN AN
62. eKAPABIOSYSTE Taq 4 3 Cat No KK3009 2 1 v17 000 250units dNTPs KK3009 2 2 va 500 1 750units Cat No KK3008 50units dNTPs si 2 000 P12 A KAPABIOSYSTEMS 2 KAPA3G Plant PCR Kits PCR KAPA3G DNA 3 PCR PCR DNA i 0C 6 Cat No KK7250 Cat No KK7251 Cat No KK7252 50 50 250 50 500 50l
63. dye BNIG 62 1 10 dye sample SWG Re LED 490nm 2 290nm 530nm r 4C 15 Cat No NE MG05 Cat No NE MG06 50 1ml 0 1 000 9 15 000 LED P68 2013 2013 10 1 12 31 info Qgenetics n c
64. 8 8A 1B D 3 ul AU Cat No NE MWP04 100 600ub 11 500 feit i itil Tris Glycine MES 1 5 TF FastGene BLUE Star Protein Ladder 4 20 12 10 Tris Glycine Bis Tris Bis Tris Heady to Use 10 180kDa Tris Glycine 10 ag C 1 4C 3 1 37 40C 3 10 1 5u 3 D 15 1 DS3
65. PippinPrep DNA sage science Cat No PIP0001 3 400 000 i gt _ 90bp 1 5kb Cat No CSD7510 0 759 with EtBr for 2 8kb EX D 10 115 000 50 2 CSD1510 1 596 with EtBr for 650 1500bp Ex A 10 115 000 80 CSD2010 2 wit EtBrfor 100 600pp FX BD OR 115 000 5 10 0 75 CSD3010 3 096 with EtBr for 90 50Dp Ex C 10 115 000 100 240V Constant 2 5A 50 60Hz CDF1 510 1 5 Dye Free for 50 1 500Dp Int K 10 115 000 535nm 640nm CDF2010 2 0 Dye Free for 100 600bp Int L 10 115 000 280x530X180mm WXDxH CEF2010 2 0 Dye Free for 100 600bp Ex E 10 115 000 7kg CDF3010 3 0 Dye Free for 90 250bp Int H 10 115 000 Ex External Int Internal Extracted from Duhaime et al
66. UV LED 2 FastGene DNA PCH i ET HI NE MGO4 1mlx1 Em aa NE AGO5 100 ER NE MWD100 DNALadder 500AlX1 Eo Ak S kb 3 hh Eh i Ht a haN mh DNA 100bp Cat No NE AG08 P4 P19 P20 9 000 P18 2018 9 1 UI HI RO es com
67. 40C 10C 1C 40C 10C WAY AC100V DC Personal DEEP FHEEZEH AC100V DC12V FPSC 48W 4A 40 10 25C 0 35 25L 335 W x 225 D x 340 H mm 695 W x 350 D x 460 H mm 15kg AC DC Cat No SC DF25 44 000 2013 2013 10 1 12 31 info genetics n co jp Q sage science i Na te re
68. P95D 1 195 000 K95HG 5 13 100 CMOS PC i i 500 10 PC FPS S 1 4V Iux sec Kr lt PC gt CPU 1 8GH z PentiumlV MANENGMMMNO mu NM 5GB 30 0 01 1 GB pe ee 1280x800 6Gmm OS Windows7 SP1 Vista SP1 XP SP3 F1 2 CD ROM USB2 0 1 PC 340X195X400mm WxLXH 0 8kg SmartView SafeBlue LED 5
69. 2013 2013 10 1 12 31 info Qgenetics n co jp eKAPABIOSYSTEAS PCR _ eKAPABIOSYSTEAS KAPA2G Fast HotStart ReadyMix with dye 30 60 PCR PCR ro I 20C 1 KAPA2G Fast 19 AS 5 95 0 75bp 5kb Cat No KK5610 2 5ml 200 25 kb 5 9 500 Cat No KK5611 25ml 2000 25 vrs 00
70. NH4 reaction Duffer 50mM MgClg 10mM dNTPs MIX 3 0C Cat No BIO 21040 Cat No BIO 21040 5 10 000 45 000 C 30 10nmol dNTPs 1 2013 2013 10 1 12 31 info genetics n cojp 17 MMyfag HS Oo Hot Start PCR Bioline GC 799 polymerase DNA 10kb TA Mix Hed dye S PCR PCR POE GC Ea 2 0C 1 26 FastPCR 340bp 530bp MyTaq Supplier F Supplier K Supplier Q Supplier S myc340 myc450 EGFR525 AGTR1 530 12 Ultra FastPCR 900bp MyTaq HS Supplier K Supplier P Supplier A 3 8 1 8 10
71. info genetics n co jp Applicafion Note 2012 10 0 1 10ng Multiplex Library Preparation Method LIMprep Ligation based lllumina Multiplex library PRHEParation method lllumina MiSea KAPA LiDrary Preparation Kit KAPA Real Time LiDrary Amplification Kit KAPA Library Quantification Kit KAPA Index DNA 0 1 10ng multiplex liDrary preparation method DNA
72. 65X1 3 5mm EX 24S 1 5ml 85x123x25mm 1 EX 24DB 0 5ml 1 5ml 865X1 3 X 5mm EX 24TR 0 2ml 0 5ml 1 5ml 85x123x25mm 1 EX MINI 0 2mlx8 0 5mlX3 1 5mlx2 mlX 85x30x25mm Sorenson W BioScience Inc FDA 10 6 10 YTDIRIELP ll jDNase RNase Pyrogen Free Free Free 77 G5ml Cat No 39900 Cat No 39930 Cat No 39960 100 80 100 Cat No 39910 Cat No 39940 Cat No 39970 200 X40 160 X32 200 X40 Chemical Resistant Marker 2
73. 1 G0 3 1ml 1 5 1 Cat No HS3000 25 000 1 Cat No HS5000RFFIL 0 45um 1 5 6 400 a HS300501 14 000 HS300502 9 Fa 9 lt HS5000RFRP 4 700 EE HS300504 14 000 5 50ml 296 ll0 a D R HIE 2 a UD a EE
74. PCR GC AT PCR PCFR e PCH PCH i 0C 1 KAPA KAPA with PCR Cat No KK2611 50 Cat No KK2612 250 Cat No KK2701 Cat No KK2702 27 500 114 000 40 000 155 000 EY KAPA HiFi HotStart Uracil Ready Mix DNA PCR IFKAPABIOSYSTEMS WGBS KAPA HiFi HotStart DNA Q
75. va89 600 PCR 3 4C 0 1C 3 4 105 0 2 55 60 0 2mlxX96 150 400 lt 0 4 gt 95C 164 000 250 B 48DA 0 2mlX48 x2 150 400 99 PCR 2013 2013 10 1 12 31 info genetics n cojp CAMLA NIPPON GRY Genetics co Ld LED LED SDS PAGE LED DNA USB CROCS SS
76. Cat No NE L2720 100 mP1 100 SE mP2 FastGene M mP 5 900 mP3 P19 1 mP4 2 mP5 mP6 10mMTris CI pH8 5 RNase A LB es 15k b 2013 2013 10 1 12 31 info genetics n co jp 100 100 25ml 25ml 40ml 50ml 25ml 10ml 10mg 10 300 300 65ml 75ml 100ml 130ml 40ml 30ml 26mg 25 Fast Gane we ylidori Green rldvancel Direct Ny P4 5 a FastGene Gel PCR Extraction DNA PCR s 2 Os e PCR DNA a 5 a tls ON
77. P 8 Y EM Tough Spot 6 280X89x150mm WxDxH Cat No HS23460 1 112800 2013 2013 10 1 12 31 info genetics n co jp la 3 196C 121C EX 24DB EX 24TR EX MINI EX 24TTV 2 0ml 85x123X25mm 1 EX 96WP 96 PCR 85x130xX25mm 1 EX 96S 0 8mIPCR
78. 3c Cat No FG 830 50 080 2 19 2013 2013 10 1 12 31 info Qgenetics n co jp Re FastGene Ready to Use 0 5g 0 5g8 50m l 19 6 96 100ml 1 0 13 0 15 0 59 1 596 61 50 1 596 88C 1 5C Protease EE3g DNA D R 5 9 Es 7 i ME 0 5 7kb Cat No NE AG05 9 0 4 6kb 0 5gx200 a 4 0 2 4kb 15 000 0 1 3kb FastGene 250bp 23kb DNA RNA
79. 8 9 16 17 24 8 48 48 48 21 250 133 000 133 000 133 000 2013 2013 10 1 12 31 info Qgenetics n co jp Bioline 7ag GC CR TA MyTaq DNA Polymerase 5XMyTaq 5mM dNTPs 15mM MgCle 3 20C 1 GC 61 DNA aaj 1 27 5 FastPCR DNA Cat No BIO 21105 500units dNTP 111 500 BIOTAQ 7ag 94kDa IA 15 30 kb dNTPs 5U BIOTAQ M DNA Polymerase 10X
80. a a i ca 1 4C 14 SI FG 9411 m FastGeneW DT 50 7 300 ae FastGeneW G50 5 x525 mg 525mg 10prep mi 2ml 50 ml DT 50ml 2013 2013 10 1 12 31 infoQ genetics n co jp GenosStaff in situ in situ 3 500 mRNA 8 actin 8 actin
81. lt KAPA library amplification kit gt PCR enrichment SE Library DNA gt Quantified by pico green Average 73 8 4 8 ng CV 0 066 ee O 0 lt Quantified by qPCR gt 1ng Library DNA gene locus n 9 Detected 8 gene locus A H lt KAPA library quantification kit gt Final QC cMiSeq Run gt SE50bp D PCR cycle lt KAPA real time library amplification kit gt Determination of PCR cycle 4 6 6 KAPA Real Time Library Amplification Kit KAPA HiFi HotStart enzyme PCR
82. 1 5 2 0ml x96 54 6X 12 1X 5cm 444 1 4 Cat No HS2345A 3 1 0 GO Scientificurc Ne 1 5ml 2 0ml 1 2ml ID 14 2X 9 6x 5 6cm cat No HS120033 DE 1 44 2013 2013 10 1 12 31 info genetics n co jp Heothrow Scientificrurc 38m W135XD105xH132mm 75m W135XD107XH13 mm Pargfilm isS are
83. 24 1 3 6 DNA CV NA MN lt KAPA library amplification kit gt PCR enrichment Quantified by pico green Average 73 8 4 8 ng CV 0 066 60 50 DNA n 9 73 8 4 8ng CV 0 066 30 20 10 The average yield of library DNA ng 0 BA lt DNA size distribution gt eid Es KAPA Library Preparation Kit End repair dA tail 3000 Adaptor ligation Kapa Library sn Amplification Kit KAPA HiFi HotStart enzyme EN KAPA HiFi HotStart enzyme
84. 4 RM A B C D 1096DMSO RM A C D 1096DMSO C D hRHM 4 ALP LEC S Mieno et al Characteristics and Function of Cryopreserved Bone Marrow Derived Endothelial Progenitor Cells the Annals of Thoracic Surgery 2008 85 1361 6 ES T Hikichi et al Differentiation Potential of Parthenogenetic Embryonic Stem Cells Is Improved by Nuclear Iransfer Stem Cells 2007 25 46 53 S Hatoya et al Effect of co culturing with embryonic fibroblasts on IVM IVF and IVC of canine oocytes Theriogenology 2006 66 1083 1090 Liu D et al Relation between human decay acceleratind factor hDAF expression in pig cells and inhibition of human serum anti pig Cytotoxicity value of highly expressed hDAF for xenotransplantation Xenotransplantation V
85. 5 980 New 15 50 ml momoy Scienkificuc 16 G0mm 12 194xX140x51mm LXWXH Cat No HS24330A AT DoS oO 1 2 7 500 Eo New 1 5 2 0ml Ceewoy Scienkificu Tough Spots 13mm 196C 150C Cat No HEAT SPOTS 50 Cat No HEATSPOTS 50 1000 1000 5 900 sz5 900 B C D G L M 0
86. 2013 2013 10 1 12 31 info Qgenetics n co jp in situ GdnoStaf HE in situ 3500 200 NBT BCIP KO TG 1
87. 22 INS 5 UU U Safe Blue SYBR Green 1 SmartView N SafeBlue SafeBlue 2013 2013 10 1 12 31 info genetics n co jp BAMBANKER a Fi FL _I 3 10 LL CUE tt 5 TL uh 3an3 oe FA imi ee atin TEE Direct
88. 28481 1 2013 2013 10 1 12 31 infoQgenetics n coJD 137 3 73 Cat No FG HP01 1 Adhesive Microplate Seal CM tude 135mmx80mm GO0C 80C i DNER s1NE gl Ee Free Free Free DNA Free Cat No 4Ti 0510 100 oge O Stick qPCR Adhesive Seal 135mmx80mm 20C 110C PCR 96 9 i INE X BNE MA el Free Free Free DNA Free Cat No 4Ti 0565 100 Adhesive PCR Seal 135mmx80mm 20C 110C PCR DNER A Free Free Free DNA Free Cat No 4Ti 0500 100 Ce t
89. Ceewoy Scientificric Pop Up Rack 5ml 50ml a e 15ml 50ml 255xX137xX7 mm WxDxH 255x137x21mm WxDxH 2 Cat No HS24320B Cat No HS24320P 2 lt 2 lt FastGene 80C 121C 0 2SEU mI 60 000XG 15ml 17x118mm 5Oml 3G0X114mm 10 1 D R MNONI EE Ll SS US Mk EE 15ml 50ml Cat No FG400 Cat No FG200 400 50 X8 200 20 10 2013 _ 2013 10 1 12 31 info genetics n cojp 133 SartOrIUS ha Proline amp i 8
90. LED LED 2 7 B USB PC RCA LED 100 240V 3A 198 xX254x227mm WxDxH 3 2kg a eC Midori Green Direct SDS PAGE CBB 2013 2013 10 1 12 31 info genetics n cojp LED DNA UIV U i NIPPON TIE Cenetics ca 12 LED FAS Didi FAS FAS Digi
91. oopQQ006 1 1 pho 1 EEE 1 ADQDQQQ0Qo000 Ss cc 8 PCR 8 PCH 2013 2013 10 1 12 31 info genetics n co jp 125 DNase RNase Human Free Free DNA Free FRAMES72 96well PCR Cat No 4Ti 0770 C 50 FRAMEStgr 8 11 000 y220 t
92. Blue Pippin 4 5 RS DNA DNA DNA DNA 5 DNA 4 3 2 1 DNA
93. 61 000 265x175x90mm WxDxH MBE 150 CABLE 13 000 150X70mm 150X100mm 150X150mm MBE LID Y1 176x268x35mm WxLxH 14 000 OG FC H 8 500 Max TO or 150 TOnmm Tay 35 140 fer 150 100mm Ta X39 a10 Gr 150 150mm Ta X35 500ml 2013 2013 10 1 12 31 info genetics n co jp SmartView lImage System SafeBlue AVD SCIGT1CG 500 CMOS Cat No MBE IMG CM SDOIDUO0 CMOS CD PC Cat No
94. Cat No 95042 566 12 42 2013 2013 10 1 12 31 info genetics n co jp New Soft Nitrile Gloves gt PA 1 pp a en _ Cat No 8 Color SA 716 SS 100 SA 716 S 100 SA 716 M M 100 SA 716 L 100 Ansell i LabTex SuperGrip 3 Color 10 10 10 10 1 100 WINDY I GLOVES Cat No Color 3025 671 ee 3025 672 3025 673 3025 674
95. Universal ABI Prism ROX Low New Bio Rad Roche Light qPCR qPCR qPCR iCycler Cycler qPCR ABI7Z500 ABI ABI7500 ViiA 7 qPCR Light Cycler 480 Light ROX MX3000P MX3005P 1 Light Cycler MX4000 GCycler iQ iQ 5 MyiQ Cycler Cat No Kit contents KK4600 1X1ml 100 20I 5 300 KK4601 1X5ml 500 20 26 000 KK4602 2x5ml 1 000 20l 45 000 KK4612 z7ZMtyk KK4613 fh KK4614 KK4615 Mt 30 20 5 300 KAPABIOSYST KAPA PROBE FAST qPCR PCR KAPABiosystems Fast 20C 18
96. ul ul 0 5 10 gt 908 oO le No 74 RR RA BH720210 BH720220 BH720250 BH720240 BH720270 12 BH720310 0O 5 10 BH720320 BH720350 BH720340 BH720370 uD 0 1 2 5 0 5 10 0 50 10 100 20 200 100 1000 1000 5000 I 36 000 BH720010 BH720015 BH720080 BH720025 BH720050 BH720070 BH720060 BH720110 50 300 NN 46 000 0 300 FastGene 4 A Ap DIRII DINE ES 121 3 Human Free Free DNA Free Cat No 10l FG 102 1 000 1 000 FG 102RS PP 96 x10 00 l FG 30 1 FG 305 FG 301 1 000 800 FG 301RS PP 96 x10 FG 302 1 000 FG 302R PP 86 x10 FG 302RS 5 PP 96
97. GC AT 20C 1 Cat No KK2801 1 25ml 50 50 66 000 Cat No KK2802 6 25ml 250 50 260 000 EY KAPA Library Preparation Kit Ion Torrent lon Torrent TKAPABIOSYSTE elon PGM lon Proton WM e KAPA HiF KK8300 KK8301 s 33i 0C 1 Cat No KK8300 KK8301 8 48 45 000 246 000 KK8310 PCH 8 40 500 KK831 1 PCHF 48 220 000 Cat No KK8330 KK8331 KK8332 KK8333 8
98. P6 Cat No NE MG05 2013 2013 10 1 12 31 info genetics n co jp DNA PCH 0 DNA PCR DNA SafeBlue DNA DNA LED FAS Digi P6 SRY 380bp TYR 255bp 2 agarose gel Safe Blue 125V 25min 10ul lane MGD P4 DNA
99. 10 11 000 550 7 500 750 riplate SW 2ml 43001 0200 es ATP Free DNase RNase Free Pyrogen Free 5 4 Cat No 43001 0020 Cat No 43001 0200 Ra 20 10 11 000 550 7 500 750 2013 lher riplate medio 1ml Cat No 43001 0101 A medio 32 P38 15 500 484 1ml 43001 1017 ATP Free DNase RNase Free Pyrogen Free Y Cat No 43001 0017 catNo 43001 1017 20 20 Ss 6 500 325 10 000 500 2ml 43001 1022 a HE3D ATP Free DNase RNase Free Pyrogen Free 5 4 Cat No 43001 0022 cat No 43001 1022 20 20 6 800 340 10 000 500 2013 10 1 12 31 info genetics n co jp 39 Work2Store Expanding
100. 18 000 w 50 000 90 000 KAPA MG Kit EC KAPABIOSYSTEMS 4 DNA XPCH 150 KAPA Express Extract DNA KAPA2G Robust HotStart ReadyMix PCR EE3g 0C 6 Cat No KK7150MG Cat No KK7153 Cat No KK7154 4 500 SU00 il2500 2013 2013 10 1 12 31 info genetics n co jp KAPA2G Fast Multiplex PCR Kit sabs22 IFKAPABIOSYSTEMS PCR PCR 20C
101. LED r Y major science SafeBlue Electrophoresis System LED MG MG SYBR Green I SYBR gold SafeView LED Cat No MBE 150PLUS MBE 150 MBE TLC 150 000 P8 m A NIPPON _ Fast ene GRE Genetic
102. TY AIO MD MINI dry bath 5C 100 PTFE 97 mm 1 9999 37 0 25C 28C 100C 19 W153xL125xH97 mm MD MINI CAR 0 6 kg MD MINI B01 MD MINI B02 MD MINI B03 MD MINI B04 MD MINI B05 MD MINI B06 MD MINI B07 Cat No MD MINI Cat No ES 29 000 MD MINI B01 0 2ml PCH x32 8 x4 5 000 MD MINI B02 1 5ml 1 5 000 MD MINI B03 15ml X6 5 000 MD MINI B04 50ml X 5 000 MD MINI B06 1 5ml 2 0ml 1 6 5 000 MD MINI B07 1 5m 82 0ml x12 5 000 MD MINI CAR Mini Centrifuge
103. 0 5ml XG6 1 5 8ml 0 2ml 6 0 2ml8 1 5 2 0ml x6 0 5 0 65ml Xx6 0 2ml x6 0 2mIl8 x2 6 000rpm 2 000xg 175x148 118mm 100 240V 0 5A 50 60Hz Cat No NE NG002B 26 400 2013 2013 10 1 12 31 info genetics n co jp JeM Heat Block 5C 150 88 RHS 832 100C 30 2 Cat No MD 02N 110 62 000
104. RF BT PES PS RF BT 2 45mm 0 85 EU ml Flow 18Ccm2 150ml 24cm2 250mD 40cmS 500mD 68cm2 1000mD RF 1 PES 180C1E 0 22 um 150ml 12 7 140 180C7E 0 22 um 50ml 12 7 840 180C2E 0 22um 500ml 1 12 880 180CSE 0 22 um 1 000ml 1 15 960 180C8E 0O 1um 1 000ml 1 15 960 BT PES 180C4K 0 22 m 150ml 4
105. i a e808 a 2013 10 1 1 1 1 a 3 i Pp i rT i 3 iu 5 SafeBlue lp MP BY ja DNA EtBr UV EtBr UV DNA EtBr UV DNA
106. IE PTFE SLPT1322NL 0 28 m 13mm 100 SLPT1345NL 0 45 m 13mm 100 PTFE amp SLPT2522NL 0 22 m 5mm 100 A o SLPT2545NL 0 45 m 5mm 100 PES Filstap ee PES 0 22um 0 45um PP SPF re Hm PES PTE Cat No Ui SLPES2522S 0 22um 5mm 100 SLPES3322S 0 22um 33mm 50 SLPES3345S 0 45 m 33mm 50 34 2013 2013 10 1 12 31 info genetics n co p i Sartorius
107. 0 2ml 8 3 0 2ml 4 99C 5C sec 4C sec 2013 2013 10 1 12 31 info genetics n co jp oy BIOer si 53 Tech nology mt CNC i LOT 6 5 640X480 262k USB USB Ver 2 LifeTOuch 96 6 5
108. 100 28 000 280 2013 Eoge DE 96well Tear A Way PCR WWW WN BLILLL Ne Co to a OY el EE Pree DA kw i NII Hm PP LU go LM 2222 Er a Cat No 4Ti 0750 TA il 50 7 20 000 400 Cheituae 0 2ml 96well PCR DIR Free Free DNA Free Cat No 4Ti 0760 50 9 500 190 Cheituae FRAME Star 96well FRAMEStar ABI Fast NE NE Free Free DNA Free Cat No 4Ti 0910 C 50 12 000 240 FRAME5S72 384well PCR FRAMEStgar nr Chituae Cat No 4Ti 0384 C 50 14 000 280 2013 10 1 12 31 info genetics n co jp 7
109. 10M read 9 3M read Q30 0 4G base 94 396 KAPA Library Quantification Kit PCHR SYBR Greenl PCR GC AT 6 DNA Total million LIMprep 2013 4 Genome Biology Yohei Sasagawa Itoshi Nikaido Tetsutaro Hayashi Hiroki Danno Kenichiro D Uno Takeshi Imai and Hiroki R Ueda Quartz Sedq a highly reproducible and sensitive Single cell RNA Sequencing method reveals non genetic gene expression heterogeneity Genome Biology 2013 14 R31T doi 10 1186 gD 20183 14 4 rd1 LIMprep KAPA Library Preparation Kit
110. Cat No FG 088WD Cat No FG 0088WD SE I 120 12 X10 5 200 6 200 y Cat No FG 088WF Cat No FG 0088WF SE 120 12 mpY5 200 T6200 FastGene PCR 8 PCR A H A 8 EES 1 5 X2 12 1 12 AV D Yl ee ei lt T I T I T Lo SE I CT I I I LT 1 d p 0 0 0n sa
111. Cat No MD 01N 110 39 000 Heat Block PCR PCR MD MP01 S MD MP02 S MD MP0O1 D MD MP02 D MD B0 5 1 5 15 000 22 000 30 000 30 000 22 000 MD B0 5 1 5 MD B0 2 MD B0 5 MD B1 5 MD B29 15 000 15 000 15 000 15 000 15 000 MD B17 MD B10 MD B13 MD B25 MD B20 15 000 11 000 15 000 15 000 15 000 teew Sontieus Gusto High Speed Mini Centrifuge 500 18 500rpm 100rpm 1 30 1 Xg RPM
112. Fi OC 2 6 4C 12 500 5 1 1 CO HyperLadderl 1kb HyperLadder V 100bp Cat No BIO 33025 Cat No BIO 33029 gt R206 9 000 14 000 TT ED i a i UD EE i pr Ee 2013 2013 10 1 12 31 info genetics n co jp 2 FastGene BLUE Star PLUS 245kDa 4 20 10 Tris Glycine Bis Tris Ready to Use Wa 5 245kDa Tris Glycine 13 20 1 4 3 1 37 40C 3 10 1 DB5ul
113. sprouting 88 5dyn cms 22 25 p lt 0 005 20 15 number of sprouting Der field 62 5dyn cm2 11 1111 1 scale 500um EEZEET 2013 2013 10 1 12 31 info genetics n co jp DNA Pippin Prep PIP0001 Sage Science IOn TOrrent POM DNA
114. x10 1000l FG 102 lt 10gpl Color FG 402 1000u 1 100 3 300 FG 402 FG 402R FG 402RS PP P 1 000 100 X10 PP 100 x10 1 300 3 700 4 500 0 PP P FG 301R FG 302R FG 402R FastGene 0 ie Ap IJNE NE Free Free FG FB205 FG FB205R FG FB205RS 1000 gl FG FB1005 FG FB1005R FG FB1005RS 1000 96 x10 96 x10 2 300 4 800 1000 100 X10 100 x10 2 600 6 600 7 200 34 2013 200 1000ul 200Ml 50 8mm 2 0mm 1 57mm 1000 76 2mm 2 65mm 2 00mm 2013 10 1 12 31
115. 2013 10 1 12 31 info genetics n co jp 80C hRM T 80C Cat No CS 07 001 5 600 250 CO1 5 1096DMSO hRM ES P3U1 K562 KATOI y oT Daudi B PC12 B OKT4 Cat N
116. 500 11 300 22 6 TR2004 50 X10 500 10 000 20 0 TR2005 x 60 X10 600 9 000 15 0 en 2 RR Free Free 10 NL NI WIT Pyrogenic TR7000 1 2ml 500 11 000 22 0 TR7001 20ml 500 11 000 22 0 TR7002 20ml 500 11 000 22 0 TR7003 5 0ml 500 11 800 23 6 2013 _ 2013 10 1 12 31 info genetics n co jp 29 rue 1 25ml TRS3000 TRS3001 TRS3002 TRS3003 TR3004 10 Lf Py 08enlc 2 Cat No TR3000 m 0 3ml 500 6 500 13 0 TRS3001 ml 0 4ml 500 6 500 13 0 TR3002 5ml 3ml 50
117. DNA EE RN NR DNA e PCR DNA Cat No BLU0001 3 400 000 ae Cat No BMF7510 0 75 Dye Free for 18 27kb Ex T1 10 115 000 BUF7510 0 75 Dye Free for 10 18kb Ex U1 10 115 000 BLF7510 0 7596 Dye Free for 1 10kD Ex 5 1 10 115 000 BDF1510 1 596 Dye Free for 6550 1500bp Int Hg 10 115 000 BDF2010 2 0 Dye Free for 100 600bp Int V1 10 115 000 BDF3010 3 0 Dye Free for 90 250bp Int Q2 10 115 000 Int Internal Ex xternal DNA 9 2013 2013 10 1 12 31 lurW FT nj El EZ 2 BluePippin DNA
118. lon Torrent POM OneTouch OneTouch PCR Life Technologies lon Torrent PGM lon Plus Fragment Library Kit DNA Covaris S2 Read Length Histogram DNA PippinPrep lt sample 1 gt lt sample gt lt sample 3 gt lt sample 4 gt L L PippinPrep PippinPrep 330bp 330bp 315bp 3 5DDp
119. 5 700 22 8 TRS3003 10ml 3ml S00 4 580 22 9 TR3004 25ml 2 6ml 100 4 800 48 0 amp lt LDPE gt _Y lnon Pyrogenic 1 Cat No TR9000 1 8cm 55cm 100 12 000 120 TR9001 3 0cm 39cm 100 14 500 145 Cat No TR9002 1 9cm 18cm 150 9 000 60 u Plate 24well IDiTreat eX l Cat No ib82406 15 ibilreat 15 iDidi
120. info genetics n co jp NN NY ar amp MP 100 R000 50V 100V 0 3kg 40W 1 100 120V Handy TOO0V 40C 0 U0 8 2 60x40x120mm WxDxH MT 108 130C a 136X188X58mm WxDXxH 400ml 9 50X83mm 105xX83mm 10mm _ _ 1 5mm 50x83mm MO 6 a 105x83mm 1 amp l 5mm 2 8mm 1 1 0mm 36mm 18004 1 1 0mm 17mm 85ul Cat No MT 100P 1 0mm 3 0mm 154 1 0mm 6 0mm 304 1 1 0mm 6 5mm 3 2 Fastln FastGene
121. ibidi lt gt gt
122. 100 100 100 N 8 A MicrO TOuch NitraTex Cat No 4 AN4470 1 100 AN4472 1 M 100 AN4474 1 100 2013 _ 2013 10 1 12 31 infoQgenetics n cojp 43 Heothrow Scientificrurc LN2 Liquid Nitrogen 1 5 0ml 82007 160 7x7x5 1cm 82007 158 12 7x12 7x5 1cm Cat No 82007 160 EAB cat no 82007 158 EAD 1 1 Optical film Compression Pad eituae PTFE 4Ti 0560 P 38
123. FastGene Stay Cool Rack 6x3mm 0C 3 4 4C 7C 596 196 96 WXDxH 99x141x44mm 4 99x141x38mm 9 Cat No FG 830 Cat No NE FG 20 50 Heathrow Scientifie Lrc Mini BinT lt amp 19X10x12cm WxDxH 153m 2013 _ 2013 10 1 12 31 infoQgenetics n coJD 35
124. KAPA Library Preparation kit KAPA Library Amplification kit KAPA Real Time Library Amplification kit KAPA Library Amplification kit KAPA HiFi HotStart enzyme KAPA Library Quantification kit iumina MiSed LIMprep Workflow lt Start material gt 10ng fragmented mouse genome DNA n 9 lt KAPA Iibrary preparation kit gt End repair dA tail adaptor ligation lt KAPA real time Iibrary amplification kit gt Determination of PCR cycle lt KAPA library amplification kit gt PCR enrichment lt Resulted Library DNA gt Quantified by pico green Average 73 8 4 8 ng CV 0 066 lt Quantifed by qPCR gt 1ng Library DNA gene locus n 9 Detected 8 gene locus A H lt KAPA library quantification kit gt Final QC lt MiSeq Run gt SE50bp LIMprep Ligation based lllumina Multiplex library PREParation method for low amount DNA using TruSeq adaptor and KAPA library preparation kit 2013 2013 10 1 12 31 info Qgenetics n co jp LIMprep Workflow lt Start material gt 10ng fragmented mouse genome DNA n 9 lt KAPA library preparation kit gt End repair dA tall adaptor ligation lt KAPA real time library amplification kit gt Determination of PCR cycle
125. Towards quantitative metagenomics of wild Viruses and other ultra low concentration DNA samples a rigorous assessment and optimization of the linker amplification method Environmental Microbiology 2012 14 9 2526 2537 Extracted from Peterson et al Double Digest RADseg An Inexpensive Method for De Novo SNP Discovery and Genotyping in Model and Non Model Speoies PLoS ONE 7 5 e37135 doi 10 1371 journal pone 0037135 2013 2013 10 1 12 31 info genetics n cojp SR P19 me Li rn a i Im al FastGene Plasmid Mini DNA 2 Oo LB _ 6 5 e5 DO mp Fast al 50ul 5bL Cat No FG 90402 Cat No FG 90502 SU lv 26 36 10 500 21 500 LB 3 2
126. 0 HeadyMix Sx GC AT PCR TKAPABIOSYSTEAMS KAPAHIFi HotStart ReadyMix T KAPA HiFi 3 9kbD 0C 1 Ready mix HotStart RT L KAPA T PR
127. 0ng 33ng 10ng 4ng 1ng 33pg 10pg 3pg Cat No BIO 21111 250units dNTP 15 600 BIOTAQ HS 7ag 94kDa 3 TA 15 30 kb 95C 10 dNTPs 5U BIOTAQIM HS DNA Polymerase 10X ImmoBuffer 50mM MgCle 10mM dNTPs MIX Sg 0C 1 Cat No BIO 21047 Cat No BIO 21047 5 26 400 wt113 000 Immolase DNA Polymerase Ray FastGene st DNA PCH EE MGO06 1mI l INE AG05 100 NE MWDIOO DNA Ladder
128. 1 Cat No KK5801 Cat No KK5802 5 1 25ml 100 25p 6 25ml 500 25p 5 12 800 41 000 ek a aaa ema 7 2x Sx 0 EA amp eKAPABIOSYSTEAS 1 KAPA SYBRY FAST qPCR Kit SE PCR GC AT SYBR Green1l Fast 20C 18
129. 11 880 96 4 X 1000ul 70610 200 3840 10 700 960 96 x5 X2 10 70620 300 3 840 12 600 768 86 4 X 8 70720 00 _ 860 10 3 500 70740 1000ul 768 8 3 500 71000 10 200 300 4 4 600 71010 1000 16 3 920 0 DTR IPI SOFCNSON gt ioScience Inc Low Binding TID MulTI Ultra G 2 s 0 1 10ul Cat No 23571T 29 96 x50 E24 000 MulTI MicroReach Tip 0110l aa Cat No 37661T 45mm 96 xX50 29 800
130. 500gIX1 5 N nm Cat No NE AG08 DNA 100bp gt 21 000 P4 P19 P20 1 Ey FastGene ig DNA PCR NE MGO04 1mlX1 INE AG05 100 NE MWDI00 DNA Ladder 500 X1 EEE 1 N mE Cat No NE AG07 DNA 100bp 21 000 P5 P19 P20 FastGene 6X3mm
131. 8 26 600 180C5E 0 22 m 500ml 1 10 080 600 700 180C6E 0 52 m 1 000ml 12 13 160 ii MM Sartolab 0 5L Sartolab 1 0L A 1 0L B 1 0L dol do do Flow ml min i Sartorius my 28mm 6 cm2 33mm elei i 3 0 2um 0 45 m Cat No 17597K Cat _No 17598K 50 50 8 200 164 vg 200 164 YY GAP CP EP 101 1mm 50 14 000 CP EP 201 L 1mm 50 14 000 CP EP 202 L mm 50 14 000 CP EP 104 4mm 50 14 000 1lcm 2cm Tip
132. D 20 ND EP GU CAN GE a 9 300 19 200 FastGene GP 100 300 EF ETE 2 ml 100 300 Cat No FG 830 1 RS 5 GP1 80ml 200ml 50 3 gt GP2 25ml 40ml 3 980 a ai GP3 10mMTris CI pH8 5 10ml 30ml P18 alsteie20 5 5 Midori Green Advance OMl 50 3 Midori Green Direct 50l 50l 2 s 300mg PCR 100 l FastGene Dye Terminator Removal WW 1 LS 20ul 10prep
133. L 22 2013 2013 10 1 12 31 info genetics n co jp Sorenson Platinum Reload System 0 1 10pl MulTI MicroReach Tip 45mm N 1 1 200l I Standardization Tip 51mm 9 N SS AO 1 300nl SV 300NX Tip 59mm 50 1000ul 1000XT Extended Tip 102mm 1 lh jp RR 121C Free Free Free DNA Free N 70500 10ul 3 840 9 800 70510 200 l 3 840 9 200 70520 200 3 840 9 200 70530 300gu 3 840 11 880 3 840 96 x5 X 2 X 4 3 07 96 x4 2 lt 4 4 70540 1000ul 3 072
134. NA contents gt 25 1 lt Quantified by qPCR gt Gene locus A og CV average 0 07 1ng Library DNA gene locus n 9 Detected CV 0 04 8 gene locus A H 20 0 8 em 0 7 KAPA HiFi HotStart enzyme 5 o DINA 5 8 6 1 locus A H PCH I EE SO 5 03 locus A 9 CV 5s 02 0 04 locusA H CV 0 07 a DNA 0 1 2 3 4 5 6 7 8 9 A B C D E F G H Library DNA name Gene locus name MiSeq lt KAPA library quantification kit gt lt MiSeq QC gt lt MiSed Run gt 000000000 AOHOH All Lanes All Reads All Cycles S E 50 b D Raw 10M read KAPA Library Quantification Kit PF 9 3M read 4 MISeq 50b p RAW
135. No GSMDP Derm 6 SPF SPF SPF Panel DBA 1JJmsSlc GSMDP Cia 6 I Panel ZDF Lepr CrICrlj GSRDP Diab 10 Genostaff st strand CDNA PCR 5 Total RNA Panel 2013 2013 10 1 12 31
136. No FAS DGDC cat No LB 16K ED 47Onm 100 50 200x160mm 185 000 67 000 J 98 000 SA OA SO RSID a a RD Cat No MB 16K UV Sm 98 000 200x160mm 4 340x280x80mm WxDxH a 4 3kg FAS Digi 350 000 UV 8W UV X5 Cat_No AP 03 218 900 AT 95SG AP 03M 6 15 900 FAS 2530M 12 000 Wg OG FC 8 500 FG 830 50 3 990 FAS 2530M FG 830 P18 OG FC 2013 2013 10 1 12 31 info genetics n co jp 7 NIPPON Genetics EY Geneticsco Lta LED DAS i 6
137. are FRAMES lt FrameStar PCR plates are covered by one or more of the following U S patents or their foreign counterparts owned by Eppendorf AG US Patent Nos 7 347 977 and 6 340 589 26 2013 2013 10 1 12 31 info genetics n co jp 0 2m 96well PCR RR Free ez WWW Cat No FG 1702 Cat No FG 1700 25 25 4 500 180 5 300 212 GG Fast Gane 0 2ml 96well PCR Cat No FG 1742 Cat No FG 1740 25 25 6 000 240 yy6 600 264 Sorenson BioScience Inc 0 1ml 96well ABI Fast IpNEE EE aITGETI Free Free DNA Free Cat No 38801 25 5 000 200 BioScience Inc 384well NX PCR INE X ENE BITTGETI Free Free DNA Free Cat No 39691
138. gistered trade mark Of American Can Company Tough Tag is a registered trademark Of Diversified Biotech 38m 100mm Cat No HS234526B 0 2ml kT 7 Ss Ss i 75m 100mm Cat No HS234526C Heothrow Scienkificrurc PCR 0 2mIPCRH 114X74X11mm Cat No HS23465A 1 gt 50ml 1 8 BB 8 Heathrow Scientific uuc 1 HS23051 90X67X67mm HS 3053A 75 9 0mm
139. o CS 02 001 RSOIC00 3 i ii CS 02 001 Cat No CS 04 001 PS human Regenerative Medicine DMSO POR C 10C iPS iPS IiPS iPS IiPS ES ES 24
140. o jp 2 NN a GD Fast ene Midori Green Advance DNA Staln Ni 0 5cm 50 60C 100ml Acivaines 4 6uL WM 0 5cm I ig oe
141. olume 14 Number 1 January 2007 pp 67 73 7 Sayyed K et al Runx2 deficiency and defective subnuclear targeting by pass senescence to promote immortalization and tumorigenic potential PNAS Vol 104 No 50 19861 SN DS CN Na OO DMSO 10C 2 9 900 2013 2013 10 1 12 31 info genetics n co jp KAPA PCR 100 BB 1 WEB 100 KAPATaq EXtra HotStart ReadyMix with dye 0C 1
142. pa jp pictbridge index_j html 4 FAS Digi HCA BNC nsW PANT RV RCA BNC VIDEO IN 2013 2013 10 1 12 31 info genetics n co jp a major
143. r 0 5 15kb and 1 50kb 20SIIAES IO IO NO ISSIMI 5 5 10X TBE Duffer DH8 0 for 3 70kb NS 108a Borle AGi BS 0 5M DTAAOmI ET 3 70kb 1 50kb 4 i 48 5kb 38 5 30 ee 1 Ee 24 5 md 1 ME 8 19 5 6 17 a 15 12 a Li 10 a 3 HH 2013 2013 10 1 12 31 info genetics n co jp Pippin DNA DNA Pippin DNA DNA
144. s co La Midori Green Direct FAS Digj FAS LED DNA 12 0 5cm 490nm 290nm 530nm EE g 4C 1 50ul 1 000 Cat No NE MG06 1ml 5 000 FAS Digi LED or UV 350 000 P4
145. t No ib80286 Cat No ib80296 15 15 U Slide 8well 8 300g4u 1 0cm jbiTreat elei i 3 Cat No ib80826 15 J Dish tbidi Low 800u1 High ml 35mm 3 5cmS 21mm Dish35mm Low 800l Cat No ID80136 IibiTreat 60 iD80131 60 Dish35mm High 6ml Cat No ID81156 ibiTreat 60 iD81151 60 3 Slide 4well 4 700
146. ude Adhesive PCR Foil Seal 135mmx80mm 40C 120C POR a lpJNEEt sNER RO El Free Free Free DNA Free Cat No 4Ti 0550 100 re tude qPCR Adhesive Seal 140mmx77mm 40C 1 10C PCR 96 384 DINE BE h Free Free Free DNA Free Cat No 4Ti 0560 100 eituae 38 2013 2013 10 1 12 31 info genetics n co jp er riplate medio 0 5ml Cat No 43001 0102 A medio 40 P38 ES 22 500 562 ter riplate plus 1ml 43001 1016 ATP Free DNase RHNase Free Pyrogen Free 4 CatNo 43001 0116 catNo 43001 1016 20
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