“WAKO BIO WINDOW” No.75(2006.6)
Contents
1. 0 00000 100 90 kDa 00 UU 0 0000000000000 18 24211 10 kDa 00000 0 4 7 30 10 CBB0 0 0 0 0 SYPRO 00000000000000000000000000000 0 0020060 50 10 0 0 70 310 0 25 500000 0000 0 0 0 0 RPMI 16401 0 0 0 00 0 0 00 0 HBSS D PB High L Glutamine and Phenol 0 0 0000 0000 MEMO 00000 00000 0 450 2 197 amp 1 Low 5 lt L Glutamine and Phenol Red 0000 0000 00000 000000 1090 000002 1 with L Glutamine and Phenol Red2. 00 Micro Smash 5 100 00000000000 8101 0 0 00 Micro Smash 5 100 0 0 000 0 0 0 000 0 0 0 0000 0 0 00000 L 0 0 0 0 90 000 35 080911 0 15 30mg 0 00000 0 15 0000000 0000000000000 0000000000000 010000000 imj 010000000 00000000 00000000 2 0 0 0 05006 lt 2 0 0 0 15006 noggin 00000002 10000000 Smm 0000000000 _ lt 00000008 81 1700090 00300 0 150009 003100 30mg 100 12019 000000000 000000000 0 40mg 15 2019 201 249 00 00000000000 00 1010000000000 30mg 50 6049 30mg 40 5019 Sample tub 17m Sample tub 17m 30mg 401 609 lt SRT 1754 lt SRT 1754 30mg 15 2019 Vortex maximum speed 15sec Vortex maximum speed 15sec 0 0 0 0 0 000 No635 o80911 000000 spindown spindown lt 95 EtOH lt 95 EtOH 17 0 00000000000000000 EE
3. 000 000 00 0000000000 eses o 00000000 638 06021 7507 00 Anti ELISA WB IHC 60 000 638 09681 4111 00 Anti CAR constitutive androstane receptor Antibody ELISA WB 50 000 632 06041 PP H8132 00 Anti COUP TF I chicken ovalbumin upstream promotor transcription factor Antibody ELISA WB IHC IP 60 000 636 06061 PP H7147 00 Anti COUP TF II chicken ovalbumin upstream promotor transcription factor 2 Antibody ELISA WB IHC IP 60 000 630 06081 7431 00 Anti nuclear receptor DAXI Antibody ELISA WB 40 000 635 09691 PP N2025 00 Anti 2 nuclear receptor 2 Antibody ELISA WB IHC 60 000 630 06101 4624 00 Anti ER estroger Estradiol receptor Ol Antibody ELISA WB IHC Rat Omi 60 000 634 06121 20506 00 Anti ER estrogen receptor Antibody ELISA WB 40 000 638 06141 5844 00 Anti ERR estrogen related receptor Ol Antibody ELISA WB IHC Rat 0101 60 000 632 06161 6705 00 Anti ERR estrogen related receptor Antibody ELISA WB IHC 60 000 636 06181 PP H6812 00 Anti ERR estrogen related Antibo
4. 0000000000000000000000 O 2100000000000000000 0 9961 961 0000000 967 000000 0 25 0000000 0000 28 30 21 247 0000000000000 lt 000000000000 1004 00000000000 DOE 2 amp Gg lt 43 O mU L L YES 2m lt YES O 0 02 0 O 70 00000000000000 0000 0 28 30 6 0 7 0 46 2 1 2 0000000 20 0000000 28 30 5 7 000000 000000 1 50 00000000 lt 01 00 0000000000049000 00000000 Sp Transformation Reagent 90u 0000000000000 00000 DNA ususse Iug C 21 0000 104000 0000000 28 3076 5 7 21 000000 376 6000000000000 000000 0 2 g 2 gt Sp Transformation Reagent 2 25
5. D E E 8 E 0050000000000 05550 0 000000 lt 0 0 4 000000 50 500 5 000 000 0000 000 0000000000000 000000000000000000000 00000000000000000000 0000000000060 00000 cDNA 200ng 0000 cDNA 1ng 5 1 50 5 0001 0 0 0 cDNA 6 5 000000000000000 1234 56 5 5 000000 000000 00 00000 0000 000000 4 000 0000 0000000000 000000000 000000000 0 0 0 000 1 654 0000000 0 0 0 000 1 000 00 2 5 cDNA 1ng 0 0000000000200 000002000 cDNA 119 69411000 00000000000 0000000 00000000 280 000 00000000 000000000 1 500700 236 0 0008 00000000 1 500700 468 000000000 1 50900 305 0 0011 00000000 1 50000 1 196 000000000 5 1 5000 246 0 0009 00000000 5 1 5900 171 455 3 429 000000000 5 1 9100 202 0 0007 00000000 1 S00 24165 4 833 0000000000 11009 0 11
6. 00000000 2091100000000000 00000000000000000000 gt 00 F200 gt Bottom gt 000 gt 00 gt 000000 gt 000 gt 000000000 gt 000000000000 M200 000 gt 0000 200 0000 gt 000000 22000 0000 00 M200 1 2001 000000000000000000000 eHO 4 DAHU 0000 0000 0000 00000 0000000 00 _ 0000000000000000000000 2001000 25000 490 M200 0 00 205 0 6681 377 669 0000000000000 000 _00000000000000000000000 0 0 0 0 M 22000 000 M200 0 0 0 C 0 00 moATP 0 00 0 3 00000 00000 000 00000000 55882611 __ 2 590 000 555 82621 4 300 000 55282631 2 4 950 000 559 82641 00000 001 2 050 000 556 82651 O O 3 240 000 0000000 2000 000 553 82661 O000000F20FA __ O 2 350 000 __ 0 0000000000000 A00 500 400 Wako BioWindow JUN 2006 No 75 13 FUJIFILM 0 000
7. 00000000 000000 lt 0 00000 1 0000 0000 000 000000 19C 000 000000 00000100000 00000 000 000 0000000 95 0000000 1 9 00000 00000 ____ 10 9 1929 1 __ 90 BALB 12 __ 95 1 5 00 000 00000 ___ __ 95 16 _5 9 2 o 20 95 NRK 9 JM404 100 00000 Jurka __ 100 90 0 0 0000000000 5 Jo e 5 88888 88888 88 88 88 88888 OUUU onoma ___5 f 000 80000005 9 0000000 0 5 wo 0 5 o es o BALB 3T3 90 o 90 ja wo 3 o _ 5 _ 00000 00000000000 000000000000000 0000 281000000000 ____
8. 0000000000000 481 00000000000000000000 LSEM 0 00000 00 00 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 58 0 0 20 4 amp 000000000000 5
9. 2 197 amp 1 1640 with L Glutamine and Phenol Red 2 12 with L Glutamine and Phenol 21 Ham s 12 with L Glutamine and Phenol Red 0 5 12 11 1100 00 0000 0000000 2 1 with Phenol Red 0 0000000000000 9000000 0 21 28 1 0 0 0000000000000000000000000 0000000000000000000000 0000000000000 00 000 00000 259000 10x D PB 01 191000000 00000 2574000 000 0 0 000000000 044 29765 0 Glucose with L Glutamine and Phenol Red 00000 500m 1 200 041 29775 D MEN Low Glucose with L Glutamine and Phenol Red 00000 500 4200 189 02025 RPMI 1640 with L Glutamineand Phenol Red 0000 500 1200 135 15175 vith L Glutamine and Phenol Red 00000 500m 1 200 087 08335 Ham s F 1
10. 199 77 siScreen 8j OOUCOTOUUU ef S 40 20 N E lt 47 00000000 000000000000000000 00 00 0000 75 0 0000 RO ORAO OG OTA O H E 000000 siRNA 0000 0000 100 100 80 90 cI 5 g 60 f lt 40 70 mm 20 60 0000000000000 000000000 15 0000000000 000 0 00 JES ALLIANCE Technology Inc siPerfect Screen 000000000000000 Kinase Receptor gt lon channel Ion binding factor Transporter Phosphatase Transcription factor O Nucleic acid binding factor Membrane transporter gt gt gt lt 10000000000 4 5000000 siPerfect Screen siS
11. gt 260 O 2221 1860 00000000000 0 000000000000000000000 0 0 0 0 0 0 0 00 0 0 0 0 00 0000 00000 00 0 0 0 0 0 1088 4 0 0000000000000000000000 0 2 4 6 8 10 12 14 16 18 20 22 24 0000000000000800000000000 J000 Fluoro GreenLys in vitro Translation Labeling Native 1 00000000000000 NativePAGE 0 Prussian Blue Stain 0 17 000000000000 0000000 3410000000000 000 0000000 0 1000000000 634 07601 4070 54 0 000 27 000 ECOS Competent E coli 1 22 20041 00 ECos Competent E coli
12. 654 0 0 0 0 0 000 000 0 0 0 0 0 0 000 0 0 0 cDNA O 0 24 165 0 0 200000 4 800 0 0 0000 0 0 GAPDHO 4 833 00 0 0 0 32100 000 0 0 00000 0 0000 0 0 0000 0 000 000000000000000000000000000000000000000 5100000000000 055090 100000000 204 62001 0500 cDNA Subtraction KitWako 90 9800 insect 1000 0000 O insect 0000000000000000000000000 24 000000000000000000000000000000000 0000210000 3 00000 0 0 00000 0 0 0 0000 00 223 2 000 00000 0 0 00000 00000000 004 0000000000 00000000000 0 000000000000000000000000000000000000 MRNA 00000
13. 2 0 00 N 0 pmol g wet tissue a 00000 0000000000 00000000000000000000000000 0 3 200 pmo 0000000000000 1 42 0 0 00 2 5 1 441 00 2 00 0 2 5 Std Mean n3 Std Mean 5 OD at 450 O O OD at 450 O O 0 0 023 255 0 0 046 126 01 005 286 15 01 0056 274 05 0083 120 05 0 097 239 w 20 0266 065 20 024 131 50 0591 272 50 0580 107 100 1132 308 100 1099 048 200 2159 220 50 100 150 200 200 2092 121 09 50 100 150 200 Std Std pmol 000 50 19 20 amp 4 000 0 0 F O 0 0 296 2 62 3 87 6 37 2 94 4 19 6 69 2 74 3 99 6 49 2 79 4 04 6 54 2 85 4 10 6 60 2 99 4 24 6 74 64401 0 0 0 93 41 94 40 96 28 92 65 82 16 98 27 06 7 30 86 8
14. 0 BA27 000000000000 pmo 1 49 0000 0000080000 4900000 O 0 0 A 45 9 45 5 564 58 8 49 1 51 2 68 1 644 E E c 624 644 83 5 799 00 405 41 2 56 1 536 E 42 1 43 5 58 0 53 7 433 454 583 547 00 G 54 7 59 5 767 7 4 51 2 54 4 709 65 8 35 40 45 50 55 60 65 9 60 70 80 9 00000 00000 pM 00000 91 200 0000 00000 1440 ELSAN H Std Mean n3 p OD at 450 0 009 2 1 0 0 083 1 73 1 5 2 5 0 054 0 00 A 5 0 0 093 2 49 1 0 10 0 0 162 6 67 25 0 0 388 7 70 05 50 0 0 859 9 14 100 0 2 031 0 67 0 200 400 600 800 1000 1000 500 80 0 100 0 Std Std 00000000 298 64601 78 000 294 64701 78 000 1000000 0000000000 20000000 00 0 000000000000000000000000 000 292 62301 298 62401 294 62501 290 62601 _
15. 0o00 10000 gt 00000000000000000000000 100 gt lt 0 000000000000000000600 0 000000000000 invitro 0000000000 Kilerkted 0 KillerRed galactosidas 10 20 30 40 50 60 000 in vitroj 0 00 00000000 0 0 00000000000000 2000 0 000000 60 gt 20 000 0005s 0 52281 00000 OUUU 25 1 99 1 0 0000000 0 0000 000 0 0000000 e gag KillerRed pKillerRed vector gt KillerRed 0 0 0 0 0 2010 oL EAT 0000 5 10 15 20 25 0 E M V Yanushevich G Staroverov B Chepumykh V M Shkrob A Lukyanov 5 and A Biotechnol 24 95 2006 000 0000000 o o 00000000 551 85141 84 000 558 385151 FP962 pkillerRedN 0 0 0 0000 000000 00 2019 84 000 55 85161 pkillerRe B vecti 00000 0 0 0 0000 00 2019 84 000 552 85171 84 000 559 85181 Cell Killer vector s pTurboGFP N vecter pKillerRed dMito vecter Iset 123 000 0 Notice to Fluorescent Protein
16. PEPTIDE 251 0 O CAR 2 4 5 1 SHP TRB 10 010000 0 00000 000 0000 00 00 00 Pyrococcus 0000000000000000000 799 0000000 994 1009 ec 9q 10041 100 O 211000000000 000 20 85 Ando S Ishida H Kosugi and Ishikawa K Appl Environ Microbiol 68 430 3 2002 000 o0 00000000 080 19871 00000 00 0 0 000 00 30 000 000000 000 0 00000000 294 6421 000 00 30 000 21 22 00 00000000060 00000 00000 000000000000000000000002 000000000000000000 0080 000000 00000000 0000000 000 0000000 0 Omi 646 09801 9944510 40 643 09811 90445 12 6 0 640 09821 9944513 10 0 647 09831 9944515 11 0 644 09841 9944516 15 0 641 09851 99445 16X 19 0 648 09861 99445 16 23 0 645 09871 9944518 28 5 642 09881 904
17. DsDD Subtraction Kit 6 Transdirect insect cell 7 0000000 Competent E coli 1 20 8 5 0 00000 00 000000000000000 9 ALLIANCE Technology siSereen p 10 Evrogen pKillerRed vector p 12 FUJIFILM 0 00000 p 14 eee p 21 OUOU 1 420ELISAQOO000 000000 008000 000 4208 05 0 0000000000 2 1 400 00 00 0 N 008000 00 400 5 0 000004 4 _ SEC 5 196 Purification 27 00000000000000 28 00000000 19 http www 1 16 Phalloidin Rhodamine X conjugated p 16 Biotum Dual Apoptosis Assay Kit with NucView 488 Caspase 3 Substrate and Sulfornodamine 101 Annexin V p 17 0000000 000000 000000000 aeree 20 0000000900000 0000000000 gt 24 26 000000 209000 13 1990 22 000 10000 00 0157 EEEE ETE 18 gogog 5 00000000 000
18. Humen R Amyloid 420 KitWako 00 78 000 78 000 78 000 78 000 20 000000000 200 0 300 0 0 00000 ProteoEnrich SEC 0 00000000000 20 1 39 00000 Protein extracts from cells tissues or body fluids ERFA RDR RR NA Novagen 5 3 40 00000000000000 000000000000000 64000000000 57990 L collect flow through 00 RIFEDI AHS LABA 40 0 00000000000000 elute with 0 100000000 5 2 1 1 2 3 s10x 0 0 0 0 0 200mv 0 50000000 2 NaCl 5mv PBS 000000 10 30 90m s BSA 100mg 0 0 0 099 0000000000 0 3m 0000000000000 5 1 0 1 Flow through 2 wash 3 eluted fraction 67 KDa 000 0 0 0 0 00000 0000 43 K
19. 5 available to Purchasers non commercial non for profit research use With purchase of Products Purchaser is granted a worldwide non exclusive royalty free limited license to use the Products for non commercial life science research only Such license specifically excludes the right to sell or otherwise transfe the Products its components or derivatives to third parties and any uses activities or the results therefrom that themselves generate revenue for the Purchaser For commercial use of the Products please contact Evrogen at license evrogen com for license information Evrogen Fluorescent Proteins Licensing Program Evrogen offers fluorescent proteins TurboGFP Phi Yellow and J Red patent applications pending for commercial use under a license Our Licensing Program is a cost effective and flexible way for customers to obtain a variety of licensing options for intemal use providing services to third parties manufacturing of novel products or other applications Quick and convenient evaluation of Evrogen fluorescent protein based technologies is easily available by purchase of fluorescent protein vectors of interest For license information please contact Evrogen by e mail at license evwrogen com I F 0 00000000000000000 10000000 2091000 2 0
20. 22 450 25 300 22 120 33 000 35 000 41 000 CORNING 100600000 37 800 10060000 37 000 44 000 33 600 0000000000000000000 0 000 00000000 00006000 0006000 000000 0 000000 00000000 0000000000000000 640 09561 647 09571 644 09581 641 09591 10 02 7077 2N 2 0 0 2 7077 5 5 0 1 0 7077 10N 10 0 2 0 00000 000000 00000000 000000000000000000000 00 644 09601 641 09611 648 09621 645 09631 642 09641 _ 10 02 7078 1N 10 0 2 7078 2N 2 0 0 2 7078 5 5 0 1 0 7078 10N 100 2 0 __ 20 __ 0 _ __ 20 0 1 290 __ 000000 00000000 000 0000000000 0000000 000 649 09651 646 09661 643 09671 640 09681 647 09691 70795 o5 02 7079 1N LO 0 2 7079 2N 20 0 2 7079 SN 5 0 10 7079 10N 100 2 0 0000 000 mi __ 25 _ 000000 00000000 0000000 0000000000000 000 640 09701 647 09711 644 09721 641 09731 648 09741 __ 10 05 70778 5 5 0 3 0 70778 10 100 40 7077 25 25 0 5 0 70778 50 50 0 3 0 00000 mO 000000 00000000 0000000 0000000000000
21. 000000000000000000000000 0 000 000000 and Wako biowin weako chemco jp 06 6201 5964 Lysate Lysate Lysate QuickGeneg 000000 lt 00000 000000 50 mO OOOO 00000 QuickGene 800 810 000 0O 00000000000000000000 000 0 0 0 8900000000000 00000000 00000000 0000000000000000 ta RNA 0000000000 00000 0 0 00000000 blood 50000 1000000000 9 0000000 D QuickGene RNA 00000 Blood QlAshodder 0 000000 36 m 2ming 0 000000 30s 000000 20000 2x100 00000000 5min 10000000000000 Blood 000000 00000 00000 43 00000 n 00000 47 8 000000 15 0000005 6100 21 000000000 50 99 0000000000000 0000000 QuickGene 000000 2000 0 00000000 6104 2m0 000 00 00000 00000 00000 504 91000 0000000 000 000000000 636 05601 800 1 632 07641 QuickGene 810 1 1 010 000 636 07921 810 00 0 0 000 110 1 498 000 633 07311 6101 1 1 480 000 632 07901 8101 0 0000 000 1110 20 000 639 07891 6101 0 00000
22. 347 90891 348 90941 348 90821 345 90831 90871 346 90861 90911 348 91041 34591051 349 90971 346 90981 90991 91001 LKOB Fluor 647 Labeling Kit NH2 B Phycoerythrin Labeling Kit LK25 Phycoerythrin Labeling NH2 SH 347 91011 Labeling Kit NH2 91021 Phycoerythrin Labeling Kit SH D 3 sample 3 sample 3 sample 3 sample 3 sample 3 sample 3 sample 3 sample 3 sample 3 sample 3 sample 3 sample 3 sample 3 sample 3 sample 00000000 21 000 21 000 00000000 12 000 12 000 17 000 17 000 21 000 21 000 21 000 21 000 21 000 27 A GEN 000 0 0 0 0 0 0000000000 0 0000 00000 FITCH TAMRA 010 000 0 0 0 00000 0 0 0000 0000 OOL Cysteine rich Peptide gt Phosphopeptid
23. 2 Binding Binding __1 Binding 5 __ Binding Pl e6 _ _ __1 Binding Pl e 7 ______ _ Binding Pl e8 __1 Binding ______ Ton Bindng Pite 10 80 _ Binding Pl e 11 _lonBinding Pl e 12 _ Binding Pl te 13 __1 Binding __1 Binding 15 _____ Binding Pl te 16 _ Binding 17 Binding 1 Binding 19 635 00571 0 Binding Plate 10 wel patex 2198 9800 80 o6 well piatex X set 632 09581 1001 Binding Plate 11 63 00501 910012 Ion Binding Plate 12 632 0961 10013 Ion Binding Plate 13 639 09611 Ion Binding Plate 14 636 09621 PSI0015_ Ion Binding Plate 15 633 09631 10016 Ion Binding Plate 16 630 09641 0017 Ion Binding Plate 17 637 09651 10018 Ion Binding Plate 18 63 09651 PSI0019 Ton Binding Plate 19 39 50 000 0000000000000 00000000000 HA http wwwwako chemcojp index htm 0000000000000000 http 00000000 000 0000000 00000000 633 07551 62024 SiScreen Custom 241611 plate 6 plates set 150 000 630 07561 12048 SiScreen Custom 48 plate 6 plates set 150 000 637 07571 912096 SiScreen Custom wall plate 6 plates set 150 000 634 07581 123
24. amp oga A 200000 O lt 5 02 0000 ole 0202 onnon A O o0 a 20000000 ooroo C 29 g AO 05 022102210505 g Sf o o 0020202102020 oton 7 E 1 g 2 oog K W 50 000 49 000 0 0 0 0 0000000089 300 0 0 0 0 000000008 3007 1 000000 000000 0 162 22011 Phalloidin Dye 547 conjugated 0 165 21641 Phalloidin Rhodamine X conjugated 0000000000000000 0000000000000000 000000 0000009 000 000 16 2 Dual Apoptosis Assay Kit with NucView 488 Biotium Caspase 3 Substrate and Sulforhodamine 101 Annexin V 1 31 000 00 488 3 00000000000000000000000000000 104 1 2 bstrat 00 DNAdye moiety 2 150 00000 OKO lt 0 0000000000 NucView substrate fluorogenic DNA dye nonfl
25. 1 gt 5mg 290 64301 296 64303 294 64304 000 00000 00 0 0 0 000 S pombe Direct Transformation Wako S cevevisiae Direct Transformation Wako 296 62 01 292 62703 290 62704 20100 10011 5001 0 0 11 5 1 57m x 1 1 5 1 125mx 2 201 4 800 100 11 000 5001 0 40 000 8 20 4800 100 10 000 5001 0 40 000 10 51 00000000000 5 siScreen 55 0000000000 0 000000000000000000000 0000000000000000 0000000 0 0 siRNA 000000000 00000 5 0 siRNA 0000000000 515 Custom 0000000000000000000000000 0 0 00 0
26. 00000000000000000000 00000000000000 BL 00000000 000000 00000000000 0000000000000000000000000 EcCos DH5a 109 000000000000 3 1 10 9 pUC19DNA DH5a 109 XLI 1 10 9 pUC19DNA 21 1 ECOS 10 0 0 60 0000000 _9 ECOS 60 000000 0 0 y 000000 2 05 0000000000 23 000000000000000000 100000000000000000000000 90000000000 5 10 0000000 429 8 0000000000 374 1 16100000 000 0 U lt e 2 0 pombe 000000000000000000000 Schizosaccharomyces
27. 40 000 63006841 PP H3210 00 Anti RXR retinoid X receptor Antibody ELISA WB Oni mgm 40 000 639 09731 PP 1665 00 Anti SF 1 steroidogenic factor 1 ELISA WB IHC Rat 60 000 636 09741 PP SHPRA OP Anti SHP orphan nuclear receptor SHPHAntibody polycional Ab ELISA WB 40 000 638 06881 6506 00 Anti TLX nuclear receptor TLXJAntibody ELISA WB IP 40 000 636 06941 0037 00 Anti orphan nuclear receptor TRZ ELISA WB 40 000 633 09751 1 7 Anti orphan nuclear receptor TR4 Antibody ELISA WB Mouse 50000 63806001 PP H2804 00 Anti TR thyroid hormone receptor Antibody ELISA WB IHC 011 60 000 632 06921 825 00 Anti thyroid hormone 1 Antibody ELISA WB Omi mym 40 000 630 06961 PP H4537 00 Anti VDR vitamin receptor Antibody ELISA WB IHC Rat Om 60 000 ELISA Enzyme linked immunosorbent assay WB Westem Blot IHC Immunohistochemistry S Supershift assay IP Immunoprecipitation 0 06 0 0 0 0 0 00000 www ppmx con products antibody NuclearReceptor index html PEPTIDE 250 00 38 Img mwm 0
28. 85571 000 0000 000 0 0 550 85873 600 401 104 0 5 50019 105 200 551 85881 600 406 104 0 00000000 000 000000 000000000 10019 46 400 1 000 0000 000 0 0 208 554 85893 600 401 105 0 5 5001 9 105 200 551 85901 600 406 105 0 00000000 000 0 0000 0 000000000 10019 46 400 000 0000 000 0 0 551 85923 600 401 106 0 5 50019 105 200 552 85931 600 406 106 0 00000000 000 00000 0 0000 000 0 0 10019 46 400 1000000 000 0000 0000 0 600 401 107 0 5 50019 109 000 600 406 107 000000000 00 0 00 000 0 0000 0000 0 10019 48 000 e aa 000 0000 000 0 0 9 555 85043 600 401 108 0 5 50019 105 200 556 85951 600 406 108 0 000 00000 00000 0 0000 000 0 0 10019 46 400 00 000 0000000 0 0 00000000 553 85961 600 401 117 01 10019 46 400 105 00000000000 000 000000000 550 85971 600 406 117 000000000 000 000000 000000000 10019 46 400 600 401 116 0 1 10019 48 000 00000 000 0000 00000 600 406 116 00000 000 00000 0 0000 000 0 0 10019 48 000 100 401 404 0 00000 0000 0 000 2001 48 000 20 06000 0 L PEPTIDE INSTITUTE INC ntan
29. 5455 Js gt 20 cO O o 0 o g S 55 851 gt HO Orin oog o o a O ooo 0 ooon Hono ogoogo _ T e z o O GA agag ZOG ogag gt lt ogo a OO oo Ooo g o oo oog 2 9 g HOTA ooo 2 o O ooo H p Oog co2000gp 5 a Oog 210000 H 9 a oo Opoo Opa po2o0o00po E g 2 808 ooog 1 O 11 4200 H H gA 2 4200 goH A goon o H N E 5 ooo Q g 24 1 O 7 I C 7502025 A Oec goro 995 JO ooo a00 aooaa
30. 634 08701 5 00022 NucleicAcidBinding 2 634 08201 631 08711 PSB0003 NucleicAcidBinding 631 08211 638 08721 PSB0004 Plate 4 638 08221 635 08731 0005 NucleicAcidBinding 5 635 06231 30 74 9800 63208741 5 0006 NudeicAcidBinding Plate 6 639 08751 0007 NucleicAcidBinding Plate 7 636 08761 PSB0008 NucleicAcidBinding 8 633 08771 PSB0009 NucleicAcidBinding Plate 9 630 08781 PSB0010 NucleicAcidBinding Plate 10 637 08791 5 0011 NucleicAcidBinding Plate 11 630 08801 PSB0012 NucleicAcidBinding Plate 12 8 637 068811 5 NudeicAcidBinding Plate 13 g wall patex ys 9800 63408821 NudeicAcidBinding Plate 14 631 06831 0015 NudeicAcidBinding Plate 15 638 0881 0016 NudeicAcidBinding Plate 16 65 08951 7 NudeicAcidBinding 17 80 96 wall plate 2 98 000 632 08961 5 0018 18 639 06871 0019 NudeicAcidBinding Plate 19 636 06581 020 NudeicAcidBinding_ Plate 20 633 068891 0021 NudeicAcidBinding Plate 21 636 0801 22 NudeicAcidBinding Plate 22 633 08911 2 NudeicAcidBinding Plate 23 630 08921 NudeicAcidBinding Piate 24 637 08931 5 005 31 50 000 H Receptor1 000 0000000 _____ O O 000000 00
31. 00 110 50 000 000 000000000 633 05611 96 33 400 637 05631 37 400 639 07151 26 500 630 07321 DB L QuickGene 1 QuickGene610L 48 44 800 639 08011 51 400 630 05621 5 0 0 0 0000 96 51 400 638 08081 52 RNA 96 43 800 638 07001 0 000 0 96 51 400 635 08091 52 000 51 96 43 800 15 20o ooo e coo coo coo o oo 8 8 Opg To 55 o 200 ZE gt coo o ooa Hoog g A D0 o ooa 5 5 54 o oo H gt O0 o 5 g Z700 858 lt 2 gt 5 5500 2 15 oOo m 2 5 5 A coo o 20 D gt 4 OO JA j gt gt o o 7 k a 55355 o0 go O G m 7 L OG a O20202 0 o 20
32. 000 65091 707881 10 05 642 09761 70788 5 5 0 3 0 649 09771 70788 10 100 4 0 646 09781 7078 25 25 0 5 0 643 09791 70788 50 50 0 3 0 00000 agi 000 0000000 00m 0000mm 0 00 646 0541 70950 5 1460 400 1000 643 09551 709509 2290 1150 1000 00000 10060000 7 000 8 000 000 _ 00000000 ___80 6540 __70 6520 __70 ___60 8700 000 _ 00000000 100 7000 100 7000 __ 7 58100 __ 110400 70 86400 000 00000000 ___50 30 100 610 7 5460 __ 5w 70 _ 84960 000 _ 00000000 50 _ 38500 __ _ 48000 ___40 _ 49600 __ _ 96000 5 2500 000 _ 00000000 ___50 _ 33500 40 4 800 __ 48000 ___40 ___ 2370 23 0000000 1 000 00000 000000000000 00000000000
33. 000000 636 09241 5 0001 1 Plate 1 633 09251 PSR0002 Receptor1 Plate 2 630 09261 PSR0008 1 637 09271 5 1 Plate 4 634 09281 PSR0005 1 5 631 09291 PSR0006 eceptor1 Plate 6 634 09301 PSR0007 Receptor Plate 7 631 09311 PSR0008 Receptor1 Plate 8 80 638 09321 PSR0009 Receptor1 1 9 96 well plate x 2 set 98000 635 09331 PSR0010 Receptor Plate 10 632 09341 011 Receptor1 Plate 11 639 09351 PSR0012 Receptor1 12 636 09361 PSR0013 Receptor1 Plate 13 633 09371 5 00 Receptor1 Plate 14 630 09381 PSR0015 Receptor Plate 15 637 09391 PSR0016 Receptor1 Plate 16 630 09401 PSR0017 Receptor Plate 17 637 09411 PSR0018 65 50000 2 000 0000000 96 latex 2 98 000 8 80 96 plate x 2 set 000 0000000 636 0981 10001 Binding Plate 1 633 0991 PS00092 Ton Binding Plate 2 636 0501 Ton Binding Plate 3 633 09511 90001 Ion Binding Plate 4 630 09521 Ton Binding Plate 5 637 09531 PS10006 Ion Binding Plate 6 634 09541 PSI0007 Ion Binding Plate 7 631 09551 PS10008 Ion Binding Plate 8 638 09561 PS10009 Ion Binding Plate 9 0 00000 0000000 _ Binding _____ _ Binding
34. 000000 00000000 eee 25 O 0 000 1900 000000 1 42 5 o 4220 0 0 40 x40 00000000 EUSA 1 amp 1420 42 0000000000000 0 11 20 e c0 0 0 0 0 BCN 1 100000000000000 O Wako 1 2 20 1 POO 50m 1 1 12 1 aaa 2m 11 0010000000 0000 0000000 0 0000000000000 5000x 9 491 5100 0 0 00000 0 0 0 0000 809 000000 0 0 0000000000000000 41000000000 1 49 1 49 0000 00 0 000 8000 240 Eusa 000 40 0 000 00 pM 2000000000 N UI 121 0 0 APPO 0000000000 0 J200 0000 2m Tris 0 0000000 298 0 00000
35. 000000000000 915 0000 000 00057 0 000000 924 omy 018 0193 012 10000000000000000 0 9157 0100000000000 9157 0000000 5001 1 0000 00 02500 000 10 10 0 601 0 000 10 00000 0 000000000000000000000000000000000000000 Ec 000 190 000 0 0 900000 000000 1002 0 0 60 0000000 22772 1000000000000000000000000 15100 6010 C Esheric
36. 2 vith L Glutamine and Phenol Red 00000 500 120 048 29785 _ Ham s F 12 with L Glutamine and Phenol Red 00000 1 200 084 08345 HBS Phenol Red gogogo 500 1 200 045 29795 00000 500m 1 200 048 29805 10x D PBS gogoo 5000n 2 300 0 Purification Kit IgG Purification 0 0000000000006 0 0 0 0 0 0 0 0 0 0 0 0 00 00 000 0000 196 00000000000 0 00 0 0 0 0 000 0 0 0 0 0 0 0 0 000 DOJINDO MOLECULAR TECHNOLOGIES INC 10000 0 00000 2000 90 196 0 0 00000 o 6110 0 0 000 30 1961 000000000 Labein 00000000000 00000 2004 9 00000 6 0000000 191 00000000000 5900010000000 Protein 200 30049 Goat 50 100u g Horse 150 20u 9 Human 150 25009 Mouse 150 20u g Rabbit 200 300p g Rat 50 100u g Sheep 50 100u g Protein G 250 350u g 150 25009 200 3004 g 200 3004 g 150 25009 150 25009 100 200 g 150 250 g 000 0000000 S 34991071 IgG Purification Kit A __ 34291061 2 000
37. 3 29 55 95 86 97 94 98 92 98 97 96 104 93 93 100 96 99 000 0 0 2 75 4 00 6 50 3 49 4 74 7 24 254 3 79 6 29 3 16 441 6 91 2 80 4 05 6 55 3 05 4 30 6 80 02 0 0 292 64501 0 96 43 99 96 96 01 98 17 97 07 92 96 93 85 89 21 4 1 000000000000000000000 0000000000000000000 1 42 000 2 5 421 00 0000 3 5 00 00 22 1 2 15 3 193 1 4 5 1 566 amp 2 000 F ao 000 1 16 0377 1 12 5 2 743 1 4 J 1 361 1 8 15 5 0 686 1 5 2 5 3 5 1 16 0 335 1 2 3 033 1 4 1 627 1 8 000 0 780 mO oOo I 000 296 64401 Human Amyloid 1 42 Kit Wako High Sensitive LI 2926551 Humen Amyloid 42 5 Kit Wako High Sensitive 0 00000000 90 000 90 000 L A A A E A C O G 1 400ELISAQOO00 OLD I 4100 8000 000 400ELISAOOOO ODOL 1 49 0000000000 000 0 0 00 00 00 0 000 0 0 00 0 000 00 00 0 00000000000000000000000000000000000000000 000000000000000 0
38. 45 20 36 0 000000 000 649 09891 99447 13 642 09901 99447 161 5 649 09911 99447 16 15 5 646 09921 190 643 09931 99447 0 24 0 640 09941 30 0 10 75 12 75 13x 100 15 85 16 100 16 15 16 150 18 150 20 150 00x0 13x 100 16 100 16 125 16 150 20x 125 20x 150 1 000 13 415 15 415 000000 00000000 00000 00000 000 0 648 09981 9944913 75 615 09991 115 645 10001 155 642 10011 190 649 10021 24 0 646 10081 300 00 13 100 16 100 16 125 16 150 20 125 20x 150 13 415 000000 00000000 00000 00000 000 0000000 O 647 09951 99448 16 115 644 09961 9944816 170 641 09971 9944819 29 5 00000 0 00006 000 0000000 00000 644 10071 999813 13 415 641 10081 999815 15 415 648 10091 999818 18 415 643 10041 9999913 13 415 640 10051 9999915 15 415 647 10061 9999918 18 415 16 100 16 125 19 5 145 0005 0000 0000 0000 0000 15 415 0100600000 8 100 9 000 11 700 13 500 15 300 18 000 19 800 11 700 15 750 00000 00 A 00000 1 000 1 000 1 000 1 000 500 500 1 000 1 000 1 000 1 000 1 000 00060000
39. 5 90 000000000 0000005 90 Wako Bio ___ ___ E mail biowin wako Jurka D 5 24 1 2100000 3100 00 00000 0 0 0 0 0 0 0 10 0000000000000 000 5 10 5 2000 0 0 0 0 0 000 10 0000 10000000000000000000000000000000000000000000 000 000000 809 000 000 00 0 0 00 0 0 00000 000 809 00000 1100000 19691 00 00000 000000000000000000 s 00000 0 0 0 00000 0 0 0 00 210 00000000000 19 0010000000 10000 00000 00 00000 0 0 0 0 00 1000000000 000 0000000 00000000 630 01601 BLC 1 000000800000000 100m 14 800 636 01603 BLC 1S 000000400000000 2 140 7 ooomimimoninimi w r KY 3 1 5 00 0000 000 00000000 204 120 637 0221 BLC 2 000000800000 100m 12800 3 00000000000000 Pro Ph tnix 005
40. 84 SiScreen Custom 384wall plate 6 plates set 150 000 637 07598 13011 5 Trial 1 24 35 000 siScreen Custom 0 Piae 0000000000000000000000000000000000000000 0000 0000000000 0 0 0 0000000000 00 Reactive Oxygen Species ROSI 00000000000000000 00000000000000000000000000000000000000000000 100000000 6000 0000000000000000000000000000000000000 00 0 in vivo 0 0 00 000000000 540 58017 O 0 00 0 00000 ROSI 00 a 00000 vv 00000000000000000000 00 0 00000 KillerRed Ca 000 lt lt nmi 585 Fluorescence 610 100 45000 804 00004 0 25 phospholipase 1 Pleckstrin Homology 113 a 00000 0 0000000 pKa 5 1 2937 00000 D 266 2 00000000 gt 00000000 2 00000000000000 1 000 400 450 500 550 600 650 700 00 nm 515 560 nm 7 W 0 000 10000 0 00000000 gt a 0 0 KillerRed 5
41. Da 0 0 0 0 0000000 000 0000000 0000000 4 12 30 20 1 14 4 Bratt et al 2001 J Chrom 909 10x amp 1 SA 0 00000000 000 00 1000000000 550 90481 715393 ProteoEnmrich CAT XSEC Kit 3800 U N IOR SOR SOR LORI LOR LEOR 5 5 5 5 5 54 5 54 5 5 5 5 5 5 5 5 5 5 5 04 0 6 190 23 00000000 Wako BioWindow 2006 75 5 00000000000 O 00 cDNA Subtraction opo 0500 Duplex specific Direct Digestion cDNA Subtraction 0 Driver 0 0 0 Tester
42. creen Custom 000000000000 00000 00 0000000 41 _ naana HeLa 9 T 47D 000 4 5 3 000 96 7 000 7 MDA MB 231 000 87 _MDA MB 4355 __ _HEK293 00000 97 ___ _ 2 ___ __ _ _00000 _______ 938 __ 2 _______000000000 __ _SHSY5SY 85 ___ hMSC 000000000000000 941 59 _ _ 850 ___ 000000000000000 77 00000000 amp 0 00 00000 0 000 0 0 00000000000000000000000 100 80 7 40 7 1000000 Oil Red 00000000000 20 50 110 180 0000 55 700000 0 0 0 0 0 0 0 00 000000000000000000000 00000000 Cy3 0 0 amp 0000000000 SK BR 30 MCF70 4355 5 0 Cy3 p 0 0 0 0 D0 siRNAQ 51 0 00 00000000000000000000 E Human kinase 1 Mouse homolog 655 5 E Human Nucleic Acid Binding Libraries 000 0000000 0000001 0 O 10000000 000 00000000 0 0000010000 634 08181 i 631 08691 0001 NucleicAcidBinding 1 631 08191
43. dy ELISA WB 40 000 630 06221 PP A9033A 00 Anti FXR famesoid X activated receptor Antibody ELISA WB IHC IP 60 000 638 09701 7921 00 Anti GCNF germ nuclear factor Antibody WB 40000 634 06241 PP H8081 00 Anti GR glucocorticoid receptor common Antibody ELISA Rat om 60000 632 06281 60 000 630 09761 PP N3224 00 Anti HNF4 hepatocyte i 50000 636 0621 2325 00 Anti LRH nuclear receptor NRSA2 Antibody ELISA WB Ra Moe 40000 638 06381 PP PPZ0412 00 Anti LXR liver X Ligand Binding Domain Antibody ELISA WB IHC IP 60 000 638 06401 PP K8917 00 Anti LXR liver X recepton B Antibody ELISA WB IP 40000 632 06421 PP H3122 00 Anti MR mineralocorticoid receptor Antibody ELISA WB IHC 60 000 636 06441 1648 00 Anti nuclear receptor Antibody ELISA WB 40 000 635 09711 PP N1404 00 Anti NGFI nuclear receptor Antibody ELISA WB IHC 60 000 630 06461 7833 00 Anti NGFI B nuclear hormone receptor NOR Y Antibody ELISA WB 40 000 634 06481 7223 00 Anti PNR photoreceptor specific nuclear receptor Antibody ELISA WB IHC Rat 00 Imm 60 000 634 06501 723 00 Anti PPAR peroxisome proliferator activated receptor Ol Antibody ELISA WB S IP 40 000 632 06541 8713 00 Anti PPAR peroxi
44. e gt Fluorophore conjugated Peptide Ser Octanoyi conjugated Peptide gt Bulk Peptide Synthesis 1 2 0 0 0 3037 3 0000 Smg 0 00098 00 CKSOGSSCSOTSYNCCRSCNOYTKRCY Disulfide bonds Cyst Reverse Phase HPLC MALDI TOF MASS 99 9 3087 3 Da 000000000 gt siyaku jutaku antibady htm G T 7 540 8605 0000000000010 20 2 0616203 1788 0000 103 0023 00000 000 00 00 0050 130 2 0303270 8243 0000 3 092 622 1005 00 e0000 2 082 285 6388 3 052m772 07884 00 28 04576 2060 3 02958 22784 e0000 022 222 3070 1 01171 0288 00 000000 O O 0120 052 099 100 O00000 0120 052 806 000000000E ODM 0 0 000 0 0 00 00 0 0 biowin http www wako chem co jp 06 218 60 01 DN
45. hia coli 25922 1175 Enterobacter aerogenes Enterobacter cloacae Enterobacter sakazakii Citrobacter freundii Klebsiella pneumoniae Klebsiella oxytoca Serratia liquefaciens Serratia marcescens Serratia odorifia Citrobacter 4 0000 0 Salmonella 0 0 0 0 0000000 100000000 30431361 NHJODO000 00 0157 10 000 G KY 0 3 1 000 MO 5 00000000000000000000000000 0000000 1 5000 1 10000 0000000000000000000000000 ELISA 1 4000 1 8000 10 0 0 000000 0501020 00000000 000 0000000 0 00000000 250 85851 e 000 00000000 0 556 85853 600 401 108 0 5 50019 105 200 557 85861 46 400
46. some proliferator activated receptor Y common Antibody ELISA WB S IP 40 000 630 06581 PP K9436 00 Anti PPAR peroxisome proliferator activated Antibody ELISA WB 5 40 000 630 06601 9621 00 Anti PR progesterone receptor common Antibody ELISA WB IHC 60 000 632 09721 4417 00 Anti PXR orphan nuclear receptor PXR common Antibody ELISA WB 50 000 000 0000000 0000000000 O 00100000 63806641 1920 00 Anti RAR retinoic acid receptor Ql Antibody ELISA WB Oni mym 40 000 632 06661 PP H4338 00 Anti RAR retinoic acid Antibody ELISA WB Om mym 40 000 63606681 PP H5620 00 Oi 40000 636 06701 PP 8740 00 Anti orphan nuclear receptor NR1D 1 Antibody ELISA WB Oni 40 000 630 06721 2729 00 REVERB orphan nuclear receptor NR1D2 ELISA WB IHC 01 mym 60000 63806761 3910 00 Anti nuclear receptor RORO Antibody ELISA WB On mgm 40 000 634 06741 925 00 Anti nuclear receptor ROR common Antibody ELISA WB IHC Rat Om 60 000 632 06781 PP H6437 00 Anti nuclear receptor Antibody ELISA WB Oni mym 40 000 632 06801 8508 00 Anti RXR retinoid X receptor Antibody ELISA WB IHC Rat Om 60 000 636 06821 7341 00 Anti RXR retinoid X receptor 3 Antibody ELISA WB Oni
47. uorescent nonfluorescent LeU GELEOC gt NucView 488 Caspase Substrate migration to 0 2 in DMSO 250 nucleus Sulforhodamin 101 Annexin V in buffer 01 BSA 02 7 5 20 250 gt Annexin V Binding 10 2 gt Ac 2 0 0 20 highly fluorescent NucView 488 00000 488 0 3 0 00 0 000 0 000 0 0 0000 0 00000 000 00 0 0 0 00 00 000 0 0 0 0 0 0 0 0 00 00000 0 000 0000 000 0 1 20 0000000 2100000 02m0 00 0 20 500000 5 3 0 0 0 0 0 2120 0000 4 PBS 10 2 0000 00 0000 00000 5 FITC 0 0 000 000 0 000 0 00000000000 00000000 30000000 00000 yua 000 00000 Sulforhodamin 101 annein VO 00000 0U UAU 0 0000 NucView 488 0000 000 0000000 __ _________ 100000000 558 93341 30030 Dual Apoptosis Assay Kit with NucView 488 Caspase Substrate and Suiforhodamine 101 AnnexinV ___ __ 65800 000000000000000000 000 0 0 0 http wwwhbhiotium cony U TN 17 0
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