CD3（lgG1）-RD1
Contents
1. 1 2 3 EDTA 6 4 90 52. 20 25 C CD3 lgG1 RD1 6604621 50 0 5mL 16 Bernard A Boumsell L Dausset J Milstein C and SchlossmanSF eds 1984 Leukocyte Typing New York Springer Verlag p 28 41 196 McMichael AJ ed 1987 Leukocyte Typing p 38 40 42 43 116 167 170 172 176 199 302 308 315 475 Reinherz EL and Schlossman SF 1980 The differentiation and function of human T ymphocytes Cell 19 821 827 Aiuta F Cerottini J C Coombs RRA Cooper M Dickler HB Froland S Fudenberg HH Greaves MF Grey HM Kunkel HG Natvig J Dreudhnomme J L Rabellino E Rittz RE Rowe DS Seligmann M Siegal FP Stiemsward J Terry WD and Wybran J 1975 dentification enumeration and isolation of B and T lymphocytes from human peripheral blood Intemational Union of Immuno
3. T EBV CMV 19 65 CD3 lgG1 CD3 lgG1 EPICS S PLUS IV n Min Max 30 56 85 72 6 5 7 mm 30 831 2 891 1 540 5 454 5 Mean 1S D CD3 lgG1 UCHT1 CD3 CD3 IgG1
4. lg E T T Smlg B lg Fc lg Smlg T B T B TdT HLA DR Smlg T B T
5. T T Common Variable Immunodeficiency DiGeorge T MS SLE B T T T T
6. MSlgG1 RD1 10L 4 45 5 PBS 2 3mL 400 450 x g 5 6 7 PBS 25 1mL 30 2 8 250 kL 9 PBS 2mL 10 400 450 x g 5 11 12 9 11 13 PBS 500L 14 EPICS 1
7. 6 7 8 9 TQ Prep Multi Q Prep TQ Prep Multi Q Prep 10
8. 11 CD3 T CD2 CD5 T 12 CD3 T T T T CD3 T CD3 13 1 10L 1 TQ Prep Multi Q Prep
9. 12mm x75mm 2 100xL 3 10L MSlgG1 RD1 10L 4 10 5 TQ Prep Multi Q Prep 6 EPICS 7 2 2 2 8 C 24 2 1 2 100L 3 10wL
10. CD3 lgG1 No Wash No Wash Q Prep Multi Q Prep TQ Prep Q Prep Multi Q Prep TQ Prep
11. 20 25KD 2 T CD3 Ti CD3 TCR CD3 Ti TCR CD3 lgG1 T NK T 20 30 CD3 B CD3 CD3 ww UCHT1 CD3 1gG1 BALB c P3 NS1 1 AG4 1
12. lg lgG1H z L RD1 Phycoerythrin RD1 Protein 0 5 1 5 486 575nm 568 590nm 1 0 5mL BSA 0 29 0 01M Nacl 0 15M NaNs 0 19 T T CD3 488nm FITC RD1 4 FS 90 SS
13. 2 Q Prep EPICS FS Q Prep 0 5mL 3 EPICS 98 1 gt CD3 lgG1 RD1 MslgG1 RD1 No Wash CD3 lgG1 RD1 MslgG1 RD1 PBS 2 CD3 lgG1 Flow Count
14. 3 4 LH 700 5 10 x10 mm 1 100L 1 Q Prep TQ Prep Multi Q Prep Coulter Immunology 3 A B C TQ Prep Multi Q Prep 1
15. B CD20 1 CD3 lgG1 CD3 lgG1 CD3 lgG1 3 EPICS CD3 lgG1 Level n Mean96 1S D 9 6CV 1 10 13 5 0 5 3 9 2 10 58 0 1 0 1 8 3 10 91 5 0 5 0 5 2 6 3 10 CD3 lgG1 Mean 1S D 9 CV 1 EPICS 84 6 0 5 0 6 2 EPICS 84 9 0 3 0 3 3 EPICS 83 7 0 6 0 8 T3 RD1 EPICS n
16. T T Harvard Medical School Dr S F Schlossman Coulter Immunology T B 3 T B T B T
17. 51 Y 0 084 1 01X 0 978 1 0 19 1 2 3 2 8 C
18. 7546999 100 3 A B C 2 1 6603152 300 PBS 24mL 1mL 2 PBS PBS 6603369 1 500mL pH 7 2 0 2 3 MslgG1 RD1 6604112 50 0 5mL EDTA 3 10 x 10
19. CD3 lgG1 STKS CD3 lgG1 mm mm x 9 x 9 10 CYTO TROL 6604248 50 Fc Fc 1 296
20. B T pan T CD7 CD2 CD5 CD3 CD3 CD4 CD8 T Pan T Pan B T B Pan T CD3 T
21. 1986 vol 26 179 188 1986 1 vol 34 173 1987 FCM vol 19 Suppl 11 38 41 1987 vol 19 Suppl 11 42 49 1987 vol 19 Suppl 11 50 58 1985 vol 8 184 191 1987 CD vol 19 Suppl 11 5 7 135 0063 5 7 TOC TEL 0120 566 73
22. 2009 11 2007 2 20600AMY00256000 6604621 877449 T CD3 lgG1 RD1 2 4 1 0 5 g T 20 000 CD3 pan T T
23. 0 FAX 03 5530 2460 135 0063 5 7 TOC Wa COULTER 6604621_0911
24. Alling DW and Steinberg AD 1982 Heterogeneity of immunoregulatory T cell subsets in systemic lupus erythematosus Am J Med 727 783 790 Smolen JS Morimoto C Steinberg AD Wolf A Schlossman SF Steinberg RT Penne E Rein herz EL Rerchlin M and Chused TM 1985 Systemic lupus erythematosus Oxford UniversityPress acute New antibodies for diagnosis of immunodeficiencies Blut 991 Immnology A ShortCourse Second Edition New JA Disatso JA and Schlossman SF 1990 iple sclerosis Analysis with monoclonal 4 4 20 21 22 23 24 25 26 27 28 29 30 31 32 33 delineation of subpopulations by clinicalserologic and T cell marker analysis Am J Med Sci 289 139 147 Morimoto C Reinherz EL Schlossman SF Shur PH Milis JA and Steinberg SD 1980 Alternations in immunoregulatory T cel erythematosus J Clin invest 66 1171 1174 subsets in active systemic lupus Morimoto C Reinherz EL Nadler LM Distaso JA Steinberg AD and Schlossman SF 1982 Comparison in T and B cell markers in patients with Sjogren s syndrome and systemic lupus erythematosus Clin Immunol Immunopathol 22 270 278 Pedrazzini AS Freedman AS Andersen J Heflin L Anderson K Takvorian R Canellos GP Whitman J Coral F Ritz J and Nadler LM 1989 Anti B cell monoclonal antibody purged autologous bone marrow transplantation for B cell non Hodgikin s lymphoma Phenotypic
25. logical Scienties IUIS Report July 1974 Clin Immunol Immunopathol 3 584 597 Foon KA and Todd RF 1986 Immunologic classifica tion of leukemia and lymphoma Blood 68 1 31 Drexler HG Gignac SM and Minowada J 1988 Routine immunophenotyping of leukemias Blut 57 327 339 Reinherz EL Haynes BF Nadler LM and Bernstein ID 1986 Leukocyte Typing ll York Springer Verlag Vo12 p 15 25 37 Caligiuri M Murray C Buchwald D Levine H Cheney P Peterson D Komaroff AL and Ritz J 1987 Pheno typic and functional deficiency of natural killer cells in patients with chronic fatigue syndrome JImmnol 139 3306 3313 Reinherz EL Meuer S Fitzgerald KA Hussey RE Levine H and Schlossman SF 1982 Antigen recong nition by human T lymphocyies is linked to surface expression of the T3 molecular complex Cell 30 735 743 Meuer SC Acuto O Hussey RE Hodgdon JC Fitzgerald KA Schlossman SF and Reinherz EL 1983 Evidence for the T3 associated 90K heterodimer as the T cell antigen receptor Nature 303 808 81O Reinherz EL Cooper MD and Schlossman SF 1981 Abnormalities of T cell maturation and regulation in human beings with immunodeficiency disorders J Clin invest 68 699 705 Schmidt RE 1989 Monoclona 59 200 206 Benjamini E and Leskowitz S York Wiley Liss p 211 244 Reinherz EL Weiner HL Hauser SL Cohen Loss of suppressor T cells in active mu antibodies N Engl J Med 303 125 129 Smolen JS Chused TM Leiserson WM Reeves JP
26. mm 10 x 10 mm 3x103 mm Q Prep PBS 20 25 C 6 a gt 10 x 103 mm 10 20 x103 2 20 30 x10 3 30 40 x10 5 40 60 x103 6 60 100 x103 10 100 200 x10 20 37 C PBS b lt 3 x 10 mm 1 20 25 C 500 x g 5 2
27. reconstitution and B cell function Blood 74 2203 2211 Preijers FWMB DeWitte T Wessels JMC DeGast GC Van Leeuwen E Capel PJA and Haanen C 1989 Auto logous transplantation of bone marrow purged in vitro anti CD7 WT 1 Ricin A Immunotoxin in T cell lymphoblastic leukemia and lymphoma Blood 74 1152 1158 Reinherz EL O Brien C Rosenthal P and Schlossman SF 1980 The cellular basis for viral induced immunodeficiency Analysis by moonoclonal anti bodies 125 1269 1274 Felsenstein D Camey WP Lacoviello VR and Hirsch MS 1985 Phenotypic properties of atypical lympho cytes in cytomegalovirus induced mononucleosis J Infect_Dis 152 198 203 Rinaldo CR Ho M Hamoudi WH Gui X and DeBiasio RL 1983 Lymphocyte subsets and natural killer cell responses during cytomegalovirus mononucleo sis Infect Immun 40 472 477 Goldstein G Lifter J and Mittler R 1982 immuno regulatory changes in human disease defected by monoclonal antibodies to T lymphocytes In Monoclonal Anyibodies in Clinical Medicine McMichael AJ and Fabre JW eds New York NY Academic Press p 39 70 Posner MR Reinherz EL Lane H Mauch P Hellman S and Schlossman SF 1983 Circulating lymphocyte populations in Hodgikin s disease after mantle and paraaortic irradiation Blood 61 705 708 Beverty PCL and Callard RE 1981 Distinctive functional characteristics of human T lymphocyte defined by E rosetting or a monoclonal anti T cells antibody Eur J Immunol 11 329 334
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