心筋芽細胞を大量に得る方法
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1. 10 20 30 40 50 J P 2008 307205 A 2008 12 25 10 10 20 GI BCO nv Cel Seed C Di ssoci ation Buffer Cell 30 40 502. o o o o a N m in m 3 3 PEN US a lt oo Se gt 3 3 3 3 x a E 3 3 3 EU a x 5 3 3 cr o oo on 3 3 3 3 3 3 Lic o m S as E 3 3 3 3 3 3 LL TZ 3 Eli 3 3 G G Oo en O 5 C C C J C 5 C C G 200 Z 3 a 3 3 3 3 3 O r3 T x UE E 3 m E Fog sof gt ana 2 d an 3 4 3 3 3 3 gt 3 3 3 3 a n 3 3 3 3 3
3. 27 J P 2008 307205 A 2008 12 25 ADMPC 00000 0000050 0 14 T 0 16 0 12 0 14 0 12 7 0 1 0 10 0 08 008 0 06 006 0 04 0 04 0 02 0 02 0 0 ADMPC ADMPC HHHHH 0 14 012 T 0 10 F 0 08 0 06 T 004 0 02 0 ADMPC Bra 28 J P 2008 307205 A 2008 12 25 00000 2 a HHHHH ADMPC ADSC 00000 6000 r 5508 5000 4000 3000 1940 2000 1000 0 0014291 0 00014 HepG2 ADSC HepG2 ADSC 00000 HHHHH 6079 95 6000 5000 800 4000 600 3000 2000 1530 400 1000 0 101 0 004969 0 01365 m i i 1 02 3 417 49 54 6 82
4. o o o o a A m in C ee oo oOo o Oo MEN E BE u lt s EX E E ES 3 3 L3 ELE oo a ea ER E oo a __ a oo 3 E 3 3 Rt fi an le WE i 3 EE E oo oong C oO oo a Oo O c oo a oO Le gt 5 oo gt o c oo O O PE a c nn Se 5 u gt I Z 3 O 1 oo O I L3 O 1 Ea c O 1 ol 3 oo 5 9 LL c O 4 E lt gt El O
5. 225 30 4 J P 2008 307205 A 2008 12 25 D
6. 10 20 30 40 50 J P 2008 307205 A 2008 12 25 12 10 20 30 DU O D n 40 Osaka University Graduate Scho 50 of Medi ci ne Revi ew Boards for Human Resear ch
7. O 13 J P 2008 307205 A 2008 12 25 Si gra Chem cal Co C HHHHHHHHHH BD Bioscience Nycored 00000000 ee z Hycl one
8. ADSC HepG2 0 7 0 5 CCL4 Bis 30 J P 2008 307205 A 2008 12 25 HHH 2008307205000001 app 31 J P 2008 307205 A 2008 12 25 5DIrt G LH IlHHHHHHHHH O000 1 09 2006 01 15 00 72 O IHHHHHHH HHHHHHHHHHH 72 IHHHHHHH HHHHHHHHHHH 72 HHHHHHHHHHH 2045 AMO BRO CBO1 CE26 48024 AAO1 ANI Co5 DAO2 HAND 48069 QUIS 4986 499 540 48065 AA90 AL2 BB11 BHO CA44 4081 AB17 BA2 494 DX FAL
9. Cel Seed trogen 11 J P 2008 307205 A 2008 12 25 HHHHHHHHHHHHHHHHHHHHHHHH in vivo in vitro
10. 50 J P 2008 307205 A 2008 12 25 3 10 IO O0 00 IO O needle injection 1 2004 Sep 14 110 11 Suppl K et al Circul ation Suzuki 459 65 Nat Med 2006 Apr 12 4 M yahara Y et al IUD Ja a I D D 20 30 40 50 IO O
11. lt r3 Ol il 3 oie mE El G _ Ss gn e 2 lt o n 5 r3 oe Oo Ol c 3 s o E 4 G 4 Ol o 2 a UE O 9 pl _ Ol r3 r3 l U Ol bad V o 5 o u a J P 2008 307205 A 2008 12 25 16
12. HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH IO000000000000050 IlHHHHHH J P 2008 307205 A 2008 12 25 2 IUD B l IU l U O n 10 IUD 20 30 I D O 40 U O
13. U O 7 JP 2008 307205 A 2008 12 25 10 20
14. 0000 ID Hs00165656_ ml Hs00184979 ml 2 2 NM_0003 32 NM 004827 Sea ABCG2 10 C Chem con G 20 HHHHHH IHH HHH U l BU O uui 30 HHHH 40 C 50 uui C
15. 50 J P 2008 307205 A 2008 12 25 6 10 20 30 40 DU O 50 uui
16. HepG2 ADSC HepG2 ADSC 29 J P 2008 307205 A 2008 12 25 6000 4000 ADSC 0 005ng 00 ng 0 5 ng 2000 0 HepG2 ADSC 3 2 04 2 HepG2 ADSC 50 ng 500 ng 5000 ng ADSC gg gg DNA
17. O 50 21 J P 2008 307205 A 2008 12 25 r3 erc kim T E 10 20 30 40 50 22
18. J P 2008 307205 A 2008 12 25 15 DU O 10 El 20 30 40 50
19. Invitorogen 4 EH D 0 0 O Invitorogen O H Invitorogenl Ou IJ E de S l Invitorogen 0n 0000000 0 een CHHHHHHHH C HHHHHHHH 20 H H D O O Applied BiosystensH 0 04000 Appl i ed Bi osyst ens H 0 U C 0 C C B 0 0 0 0 Applied Bi 30 osystens Seo M et 2005 Mar 4 328 1 258 64 e E 18 J P 2008 307205 A 2008 12 25 ID ID ae TERTEFA zx NM 001134 Hs00173490 ml PAS Y NM 000477 Hs00609411 ml P450 1 NM 000104 Hs00164383 m1 PPS es UH By Ri1 CYP1B1 FZu uz Pp450 Zy NM 000767 Hs00167937 gl B 6 NM 002063 Hs00374213 ml PDF WI NM 199187 NM 000224 Hs01941416 gl 1 9 NM 002276 Hs00761767 s1 Nacalai Tesque 0 Uj 00
20. m 17 J P 2008 307205 A 2008 12 25 Q AGE L Ed f AG ua MES e fo a ELTON E u BE x o n u uoog u H H n n 10 Inv torogen 0 I 240000000 Invitorogen hu C x
21. Nu pee Be o REZ HEE EL NIE L c Sano IS xov ool 05 of OF OF 0 O El zune To 5 8888 Cr 60 00 24 J P 2008 307205 A 2008 12 25 HHHHH 2 10 2 2 00000 6 2 HHHHH 80 60 40 20 2 00000 607 407 201 2 25 J P 2008 307205 A 2008 12 25 26 J P 2008 307205 A 2008 12 25 00000 HHHHH
22. 3 3 5 gt onog 3 G 3 3 3 3 a 3 3 3 5 3 o 3 E eo X m 3 3 3 3 3 3 lt S 3 3 3 3 3 5 E 3 oo Y 3 3 3 lt a 3 Q R a il i 2 3 3 E rrr g oY 2 ol O 1 J P 2008 307205 A 2008 12 25 20 lOO Ig O BU O 10 20 30 40
23. J P 2008 307205 A 2008 12 25 UOU0O000000000000000000000000000000000000 HHHHHHH HHHHHHHHHHHH HHHHHH SEQ I D 1 Islet 1 forward pri mer SEQ ID 2 Islet 1 reverse pri ner SEQ I D NO 3 Nkx2 5 forward pri mer SEQ ID NO 4 Nkx2 5 reverse pri mer SEQ ID NO 5 GATA 4 forward pri mer SEQ ID NO 6 GATA 4 reverse pri mer SEQ ID NO 7 q CA forward pri mer SEQ ID NO 8 a CA reverse pri mer SEQ ID NO 9 M C2v forward pri mer SEQ ID NO 10 M C2v reverse pri mer SEQ I D NO 11 MHC forward pri mer SEQ ID 12 MHC reverse pri mer SEQ ID NO 13 GAPDH forward pri mer SEQ ID NO 14 GAPDH reverse pri mer 0 u u u u ADSC ADMPC ADSC ADMPC MLC MHC MLC MHC CA lt S GAPDH lisl 1 GAPDH Isl 1 Nkx2 5 GATA 4 Nkx2 5 GATA 4 ADSC ADMPC J P 2008 307205 A 2008 12 25 0000 23 0000 A GUB HELL lE L vo yuo IE eZ v VLVD HEL las mm ns G ZX9MHMN mm nc HE pre mE Wi mes AIS AS gt WB WEL nal dy Del CN Eo co Ec 8 5 38888 BS Bee E 7 HE S S NC 3 260 5 B G G G 3 Luma lt EE L EIE L a BOL i NB WBL TE iis gs NET BBC
24. OOOOOOOOO00000000000000000000000 nnnnnnn H H aa HHHHHHH uu TOYOBO Invi trogen gc 0000000000000 000007TOYOBO nnnnn nnn Cnn nn eCH C EET Mr AI c am m E iW HHHHHH C lis let GTCAGTGGTGGACCT GACCT 1 6 0 AGGGGAGATTCAGTGTGGTG 2 Nkx2 5 GGTGGAGCTGGAGAAGACAGA 3 6 0 CGACGCCGAAGTTCACGAAGT 4 GATA 4 ACCAGCAGCAGCGAGGAGAT 5 60 GAGAGATGCAGTGTGCTCGT 6 vw CA GGAGTTATGGTGGGTATGGGTC 7 6 0 AGTGGTGACAAAGGAGTAGCCA 8 MLC 2v GCGCCAACTCCAACGTGTTCT 9 6 0 GTGATGATGTGCACCAGGTTC 1 0 MHC GGGGACAGTGGTAAAAGCAA 11 6 0 TCOCTGCGTTCCACTATCTT 12 GAPDH GTCAGTGGTGGACCT GACCT 13 6 0 AGGGGAGATTCAGTGTGGTG l4 10 20 30 40 50 J P 2008 307205 A 2008 12 25 14
25. 0 PIERCE D000000000000000000000000000000000000000 Nacalai Tesquel O O C O DOE PERCEH 000000000 J P 2008 307205 A 2008 12 25 19
26. 30 40 50 J P 2008 307205 A 2008 12 25 8 BU O 10 20 30 40 50 U 9 J P 2008 307205 A 2008 12 25
27. JP 2008 307205 A 2008 12 25 19 JP a2 4s Bd Fe FF BRA 11 2008 307205 P2008 307205A 43 2 12 5 2008 12 25 51 Int CI A61L 27 00 2006 01 A61L 27 00 E 2G045 C12N 5 06 2006 01 C12N 5 00 ZNAE 4B024 C12Q 1 02 2006 01 C12Q 1 02 4B063 GOIN 33 15 2006 01 GO1N 33 15 Z 4B065 GOTN 33 50 2006 01 GOIN 33 50 Z 4C081 C 12 OL 31 21 Hees 2007 157389 P2007 157389 71 HERA 504176911 22 19 6 14 2007 6 14 1 1 74 100081422 74 100084146 74 100116311 74 100122301 EH BR 72 E I 1 1 hh B ji 54 520000 HHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
28. O OD 10 0 uu uu 0 a car ht Chain 20 H nn H nn 30 na n sd RS _ 40 50 J P 2008 307205 A 2008 12 25 5 10 DU O 20 30 C 40
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