Fluo-4 NW Calcium Assay Kit（F36205、F36206）
Contents
1. Fluo 4 AM Fluo 4 AM CK Molecular Probes Fluo 4 NW No Wash Fluo 4 NW fluo 3 fluo 4 Molecular Devices Calcium 32. fluo 4 NW A Cli 26 000 RFU 2 13 000 RFU fluo 4 NW HEK293 Jurkat CHO flhno 4 NW Calcium 3 ZEO CHO M1 3 EC 2 0 EC fluo 4 NW 22 6 nM ino 4 15 9 nM Calcium 3 19 7 nM
3. fiuno 4 Fluo 4 NW pH 7 7 5 Ca 1 MP 36205 Fluo 4 NW Ca
4. 10 150 mL 3 mL 1 M HEPES 147 mL 1 HBSS 150 mL 2 2 96 1 125 000 384 1 31 250 ge EYL Jurkat GeneBLAzer M1 NFAT bla Jurkat K1051 Bil 1 5 x 10 mL 96 100 384 400 12 5000 x 100 31 250 x 400 1 25 x 107 Al
5. fluo 4 4 uM 37 C 30 30 200 nM FlexStation 1 Molecular Devices 4 Fluo 4 NW Calcium Assay Kit 3 8 S Cc D D S D no probenecid Z m a s x water soluble form A Calcium 3 7 7 acid form 0 10 10 102 10 0 11 19 26 34 41 49 57 64 72 79 Carbachol nM Time seconds 2 CHO M1 3 2 5 mM CHO M1 37C 60 CHO M1 20 nM
6. 37C 37 C 30 45 1 8 494 nm 516 nm 488 nm FE MAORA F I L A MD 23 High throughput kit 2 1 Starter pack Component C 2 2 2 1 High throughput kit 10
7. 5 mM High throughput kit 50 mL 1mL 1 Component A 2 10 5 mM 1 2 2 9 C 2 10 50 uL 2 96 12 5 uL 2 384 2 11 212 37C 30
8. fluo 4 NW 4 BAXE 1 Cell Calcium 27 97 2000 2 Biotechniques 34 164 2003 F36205 Fluo 4 NW Calcium Assay Kit high throughput for 100 microplates F36206 Fluo 4 NW Calcium Assay Kit starter pack with buffer for 10 microplates P36400 ke ce F14202 fluo 4 AM packaged for high throughput screening lt Fluo 4 NW Calcium Assay Kit 1 kit 1 kit 10x 77 mg 5x 1 md Contact Information Further information on Molecular Probes products including product bibliographies is available from your local distributor or directly from Molecular Probes Customers in Europe Africa and the Middle East should contact our office in Paisley United Kingdom All others should contact our Drug Discovery Solutions Technical Service Department in Madison Wisconsin or the Technical Service Department in Eugene Oregon Please visit our websites probes invitrogen com and www invitrogen com drugdiscovery for the most up to date information Molecular Probes In
9. 2 5 mM 1 2 1 5 C 1 6 100 uL 96 1 25 x 10E 25 384 1 7 18 SF VH he 376 30 30
10. 3 mL 1 M HEPES 147 mL 1 HBSS 150 mL 1 3 250 mM 1 mL 1 Component B 20C 6 1 4 Starter pack 10 mL 100 uL 1 Component A 1 TRC OO Take CS 7 aes 2 5 mM High throughput kit 100 mL 1mL 1 Component A 1 10
11. R795 07 96 CHO HeLa 3T3 1 30 000 40 000 HEK293 1 40 000 50 000 1 384 1 96 14 1 22 B HOR High throughput kit 1 2 BFF Starter pack FCHMHAOGER FvrIaNvyI7r ls Component CELTHRBLFIODC KAFYT 1 3 BEARS LEl EH Component A AU THHL EEARBRIA 14 BF BHLESAILE AALOS EES BHBROAK ERLI SED FL K 2MbFAUSEE starter pack F KAOG GB 10 OFF BROFL hREFAUSGER high throughput kit TEADA FHAWEL FF 1 2 High throughput kit 10 BO 10 150 mL
12. 30 37C 37 C 30 45 2 12 494 nm 516 nm 488 nm HRM cha fluo 4 NW fluo 4 Calcium 3 Molecular Devices CHO MI 2000M OV fluno 4 NW X 1 fluo 4 NW
13. 1 5 x 10 mL SS ML OE RES eH i 2 3 1 000 rpm 200 x g 3 24 2 5 x 10 mL 96 125 000 50 nuL 384 31 250 12 5 uL 50 pL 12 5 pL Fluo 4 NW Calcium Assay Kit 2 96 S0 uL x100 mL 384 12 5 uL x 400 5 mL 25 FRM Le Me 1 50 pL 12 5 uL 26 5 CO 3
14. 100 9 2 MME FAA ARAW ETS Component A Component B 1 1 Re starter pack 10 high throughput kit starter pack 10 high throughput kit Fluo 4 NW Calcium Assay Kit RB tMIMAOZRTO KIA L A MIDE 1 1 96 384 poly D Lysine So PC SVE bin Cee eg Se CTS M1WT3 CHO M1 ATCC CRL 1985 GripTite 293 MSR HEK293
15. HEK293 EC fluo 4 NW 13 6 nM fluo 4 14 1 uM Calcium 3 19 0 uM Jurkat fluo 4 NW EC 13 nM Calcium 3 EC 12 nM CLE fluo 4 NW 3 10 x 10 9 x 10 8 x 10 7 x 10 6 x 10 5 x 10 4 x 10 3 x 10 2 x 10 1 x 10 Relative fluorescence 5 13 20 28 35 43 51 58 66 73 80 Time seconds B41 fluo 4 NW fuo 4 Calcium 3 M1 CHO poly D Lysine 96 fluo 4 NW Calcium 3
16. provided that neither this product nor any of its components was used in the manufacture of such product If the purchaser is not willing to accept the limitations of this limited use statement Invitrogen is willing to accept return of the product with a full refund For information on purchasing a license to this product for purposes other than research contact Molecular Probes Inc Business Development 29851 Willow Creek Road Eugene OR 97402 Tel 541 465 8300 Fax 541 335 0504 Several Molecular Probes products and product applications are covered by U S and foreign patents and patents pending All names containing the designation are registered with the U S Patent and Trademark Office FlexStation is a trademark of Molecular Devices Corp Copyright 2006 Molecular Probes Inc All rights reserved This information is subject to change without notice Fluo 4 NW Calcium Assay Kit 5
17. 7 C 60 B HRORR Component A 2 8 starter pack 10 high throughput kit ye Lad 2 7 250 mM Ab y ZRII 1 mL 1 Component B 20C 6 2 8 2 Starter pack 5 mL 100 mL 1 Component A CV 2 1
18. Molecular Probes invitrogen detection technologies 2006 10 A 20 AFT Fluo 4 NW Calcium Assay Kit F36205 F36206 F36205 Fluo 4 NW Calcium Assay Kit high throughput for 100 microplates F36206 Fluo 4 NW Calcium Assay Kit starter pack with buffer for 10 microplates e Component A 20 C e Component B 25 C e Component C Starter pack 2 6 C 20 C 494 516 nm Fluo 4 AM Ca HTS Ca Fluo 4 AM G GPCR
19. c Drug Discovery Solutions Technical Service 29851 Willow Creek Road Eugene OR 97402 Phone 760 603 7200 ext 40266 Phone 541 465 8300 Fax 541 335 0504 Invitrogen European Headquarters Customer Service 6 00 am to 4 30 pm Pacific Time Invitrogen Ltd Phone 541 335 0338 Fax 541 335 0305 probesorder invitrogen com 3 Fountain Drive Inchinnan Business Park Toll Free Ordering for USA Paisley PA4 ORF UK Order Phone 800 438 2209 Order Fax 800 438 0228 Phone 44 0 141 814 6100 Fax 44 0 141 814 6260 Email euroinfo invitrogen com Technical Service 8 00 am to 4 00 pm Pacific Time Technical Services eurotech invitrogen com Phone 541 335 0353 Toll Free 800 438 2209 Fax 541 335 0238 probestech invitrogen com Molecular Probes products are high quality reagents and materials intended for research purposes only These products must be used by or directly under the supervision of a technically qualified individual experienced in handling potentially hazardous chemicals Please read the Material Safety Data Sheet provided for each product other regulatory considerations may apply Limited Use Label License For research use only Not intended for any animal or human therapeutic or diagnostic use The purchase of this product conveys to the buyer the non transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer whether the buyer i
20. lcium Assay Kit 1 M NaOH Fluo 4 NW Calcium Assay Kit HTS Fluo 4 NW dye mix Component A Component B 1 x HBSS 20 mM HEPES Component C Starter Pack BERI SABIE lt 6 4 amp 6 high throughput pack A 15L0 1X HBSS 500 mL GIBCO 14025 092 3 e 30 mL 1 M HEPES 20 mL GIBCO 15630 106 2 Fluo 4 NW Calcium Assay Kit starter pack with buffer F36206 10 Component C Fluo 4 NW Calcium Assay Kit for high throughput
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