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Contents
1. gal gal gal2. 8 gal 96 Step 1 Lumi Gal 530 Step 2 gal Step 3 50 Step 5 5 lt 10 10 15 Step 6 20 Step 7 1801 Lumi GalS 530 Step 8 37 C 30 Step 9 5 lt 10
3. 1 MDCK 2
4. J Alam and J L Cook 1990 Analytical Biochemistry 188 245 254 E G Beale E A Deeb R S Handley H Akhavan Tafti and A P Schaap 1992 Biotechniques 12 320 323 1997 CURRENT PROTOCOLS TI 288 298 D C Young S D Kingsley K A Ryan and F J Dutjo 1993 Analytical Biochemistry 215 24 30 2 Lumi GalS 530 100 ml 150 ml 0 1 ml Lumi Gale Lumigen Inc Lumi GalS 530 20 4 C 20C 10
5. galactosidase 1 2u 1 10 1 20 1 gal 4 DNA gal A gal gal
6. 50 30 8 gal HPCR 530 8230 2 8 TEL 06 6348 3786 FAX 06 6348 3833 E mail order_lifescience toyobO jp 141 8633 10 2 TEL 03 6422 4819 FAX 03 6422 4951 E mail order_lifescience toyobO jp TEL 06 6348 3888 FAX 06 6348 3833 9 00 12 00 13 00 17 00 E mail tech_osaka toyobo jp URL http www toyobo co jp bio
7. 4C 2 gal es 5 PB Reporter Assay Kit gg 4 fi 37 C 30 60 3 PBS X 96 1 5ml 96
8. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Step Step 8 Step 9 Step 10 Step 11 Lumi GalS 530 gal PBS 1 PBS 35mm 200 gt 250 5 lt 10 37 C 1 3 1 5ml 1 5ml 4C 15 000
9. 8 gal pH7 3 gal pH5 pH 8 gal ONPG 5 pH pH gt 9 gal
10. ldeas amp Chemistry Reporter Assay Kit 6gal Code No BAK 101 TOYOBO CO LTD Life Science Department OSAKA JAPAN 1 2 3 4 OMN VY 1 Reporter Assay Kit gg gal jj gal 2c
11. rpm 2 20 96 20 C 1 2 3 180 Lumi Gale 530 37 C 30 lt 60 8 gal 0 15 lt 90 30 5 lt 10 1 5
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