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1. AGPC Total RNA mRNA RNase RNA RNase Inhibitor2. 0 1 Diethylpyrocarbonate DEPC 37 C 12 121 C 30 7 1 mRNA 1 2 2 ME 2 1 800 pl 2 5 6 ul 1 400 pl 2 2 8 pl 2 1x10 cell 1 5 ml 400 2 ME 20 25 G 1 ml 10
3. 1 10 5 1 2 2 ME 2 1 ml 20 25 G Mag ca 77gpoe Code No MGS 101 65 C 3 000 5 000 rpm 6 RNA 1 RNA RNase RNase RNase 2
4. 2 3 3 mRNA 1 250 ul B F 10 min B F 1ml B F 1 ml B F 1 ml B F 90 ml 65 C 2 min
5. RNA 800 hl p 3 3 1 5 ml 400 2 ME 1 5 ml 20 25 G 1 ml 10 RNA 800 pl 10 000 rpm x 3 min
6. 15 000 rpm x1 min 7 10 A RNA RNA ng A x x 40 2 Total RNA mRNA Total RNA AGPC Acid Guanidinum Pheno Chroloform MagExtractor RNA Total RNA 1 2 2 ME 2 1 800 pl 2 5 6 ul 1 400 pl 2 2 8 pl 2 Total RNA DNase I Total RNATT2493 x 100ug 88 x l 10 x DNase I Buffer 10 RNase Inhibitor 10 units 1 TAI DNase I 1 unit pl TAI 100 1
7. 1x107 cells 50 mg 100 mg Total RNA 100 hg 4 5 mRNA 4 ml 8 ml 30 ml 5 ml 2 5 ml DNase I 1 unit pl 10 ul RNase Inhibitor 10 units ul 10 ul 10 x DNase I Buffer 100 ul 4C 4C 4C 4C 4C 20C 20 C 20 C 2 400 2 8 v v
8. 15 000 rpm x1 min 11 10 20 Azs RNA RNA ng A x x 40 8 Total RNA 100 ug 1 3 hg 1x107 cell 1 3 pg 30 mg 1 2 ug 30 mg 0 5 1 hg 40 mg 0 5 1 ug 100 mg 0 5 1 hg 11 9 1 RNA RNA 2 RNA RNase RNA DNase
9. PCR DNA Azo Azs 5 13 10 Code No MGS 101 HMX 301 MagExtractor RNA Total RNA NPK 201 RT PCR Quick Master Mix One Step RT PC
10. 8 1 20 30 gl 9 1 10 A260 RNA 15 000 rpm x1 min RNA ng A260 x x40 4 mRNA 2 250 ul B F 1 mRNA 10 min B F 1ml B F 1 ml B F 1 ml B F 20 30 pl 65 C 2 min B F 10 10
11. 72 DNase I RNA DNase DNase DNase 80 RNA 12 3 RNA 4 RT PCR DNA DNase I DNA DNase I RT PCR
12. B F 100 ul 65 C 2 min B F 1 400 pl 2 ME 800 2 1 mRNA 5 20 6 15 000 rpm X30 sec 7
13. 1 oligo dT mRNA poly A RNA mRNA Total RNA mRNA RT PCR cDNA cDNA MFX 2000 2100 6000 9600 lt gt mRNA Total RNA mRNA DNase I 2 mRNA 2 mRNA RT PCR cDNA cDNA
14. mRNA DNA DNase I rRNA 2 1 RT PCR 2 RT PCR cDNA cDNA 1 DNA DNase 2 1 rRNA DNA 1 1 3 1 5 MagExtractor mRNA mRNA eg cec gt gt Wo ov oo ov rRNA tRNA genomic DNA mRNA AAAAA TTTTT OQ oligo dT OK So AAAAA TT Nass DD DNase 1 3 MagExtractor mRNA
15. 15 400 1 2 ME 800 1 Total RNA 1 ng 2 Total RNA 100 ng 3 DNase I Total RNA 2 4 DNase I 3 mRNA 1 250 ul B F 9 DNase Total RNA 2 10 min B F 1 ml B F 1 ml B F 1 ml
16. B F 1 mRNA 4 DNase I 1 mRNA 90l 10 x DNase I Buffer 10 RNase Inhibitor 10 units pl 1 l DNase I 1 unit pl 1gl 102 1 20 2 15 000 rpm X 30 sec 3 4 1 20 lt 30 10 20
17. Azs RNA RNA ng H Azxaox x40 15 400 2 ME 800 pl 5 mRNA 2 250 ul B F DNase I 1 mRNA 10 min B F 1ml B F 1 ml B F 1 ml B F 20 30 pl 65 C 2 min B F 5 DNase I RNA 6
18. 10 04 mRNA Purification Kit MagExtractor MRNA Code No NPK 801F TOYOBO CO LTD Life Science Department OSAKA JAPAN A4123K 1 2 3 4 5 6 RNA 7 1 mRNA oo 1 2 3 mRNA 1 4 DNase 5 mRNA 2 2 Total RNA mRNA ES NR WP WH WR FR HRP MR Total RNA DNase mRNA 4 mRNA 2 8 9 1 RNA 2 RNA 3 RNA 4 RT PCR 5 10 1 2 3
19. R PCR 311 ReverTra Dash RT PCR PCR 401 ReverTra Plus RT PCR PCR 501 ReverTra Ace w cDNA FSK 101 PCR cDNA TRT1o1 SIN 10 NTP 301 KOD FX KFX 101 LDP 101 KOD Plus KOD 201 KOD 211 Blend Tag BTQ 101 BTQ 201 QPK 101 QPK 201 QPK 212 QRT 101 QRT 201 THUNDERBIRD Probe qPCR Mix 1 67ml x 3 QPS 101 200 THUNDERBIRD SYBR gqPCR Mix 1 67ml x 3 200 QPS 201 14 530 8230 2 8 TEL 06 6348 3786 FAX 06 6348 3833 E mail order_lifesclience toyobO jp 141 8633 10 2 TEL 03 6422 4819 FAX 03 6422 4951 E mail order_lifesclience toyobO jp TEL 06 6348 3888 FAX 06 6348 3833 9 00 lt 12 00 13 00 17 00 E mail tech_osaka toyobO jp URL http Www toyobo co jp bio
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