BIAevaluation （Ver.3、4共通）
Contents
1. 3 3 1 15HII ee T1400 13500 13000 12500 12000 ab00 400 BODD HH 1 12b00 1400 1BII 1 ab0b0 BIAevaluation Software Handbook 1 TS 9 T1400 13500 13000 1250D0 12000 ab II BODD HI 1IH 1200 14DD TE TSOD Edit Copy Copy Graph Edit Paste Curve St FE riginal Fositor el Lanmcel Se 1 5 ln tr ln Mew PEolour Specif2. Next gt Average Fit enera am a 125m 5 BIAevaluation Software Handbook 45 3 Affinity Fit NO Pardmeters Report Farameters RESsicluals Selections J Req SE Reqj GLCNAC Fc 1 1 164 0 0454 GLCNAC Fc 1 2 131 0 0454 GLCNAC Fc 1 3 100 0 454 GLCNAC Fc 1 4 747 00454 Fit Plot Parameters SE Lnric La T Wals Hgn Conc Y Values Req OK BE 1 E 4 EE 4 4 1 1 BIAevaluation Software Handbook 46 3 Affinity Fit General
3. OO 4 Fa ia Rlg sarnpnle 1 Feptide ble ee Enntrnl Feptide ble 1 samplel z camwel 1 2 Se RU 350 S ml 1D0 150 ab0 250 Tie ET ET TSE ET ia ETEIgEnIBTI TEEIEEIimr TN A ET EE 1 3 9 BlAevaluation Software Handbook 13 1 Curve Curve 2 Blank Run Subtraction Curve 2 2 control Transtoarm X Uperation Henlare Drginal ero at verage of Selection ero at Median of Selection dd As Mew Curve Value vauel Lurye lt walue os Lancel Ln Curve 1 Lurye LnfY0O YI TU at Cursor Fosition ft Curve Curve 2 Blank Hun Subtraction ruvez zeool I Apply to Entire Working Set Replace O
4. Global fitting Local fitting EAN 2 1 3 BlAevaluation Globol fitting P23 gt P29 Local fitting P31 Global fitting Kinetics Local BIAevaluation Software Handbook 22 2
5. RU Rmax Response O 11 0 O lt _ Rmax Global fitting Fitting BIAevaluation Software Handbook 33 2 Kinetics Response TO 5 1ODD 7 1 1 2BD Sb 230D rt Time TE EG GD Rmax EO RI RU Cone of analyte KA 17M KD M Req RU _ Local fitting Response ka 1 Ms1 kd 17s Rmax RU RITIRU Conc of analyte KA 17M KD M Reg RU 5 1 1 3 1 3 141 5 1 D 2 1 U
6. ko Y ky k 0 C Program files BIAeval Anr22 150 2D0D 25 3HH 35 OD 5 SO EO 5 Tirmnme BIAevaluation Software Handbook 39 2 Kinetics Fit Kinetics Separate k k Association RU 160 140 120 ae Ft Finetics Separate 1 80 60 En SE Pl 20 2 150 ao00 2 Next gt Ft Kinetics Separate ax 1 1 sngrnuir association hfeea hlrr FC 1 Hf22a hlr 8 FC 1 apraga blr 9 FC 1 hfega hlr 1 1 apr22a hlr 11 FC 1 CC 1 1 Langmuir rere Se BlAevaluation Software Handbook 40 2
7. Linear fitting Non linear fitting BIAevaluotion BIAevaluation Software Handbook 20 2 Kinetics Kinetics Kinetics Global fitting 2 1 1 en i a Local fitting 2 1 2 IITTUItOTIEOUS TITLIN 2 1 2 1 3 Separate fitting 2 2 Simultaneous fitting Separate fitting Curve fitting ko Curve fi
8. Pe OFF TEL 03 5331 9336 ED 9 00 17 3
9. Ka MD Kp M A B AB Kpl Kp A B AB Ka 1 Kp Affinity Affinity Affinity Affinity Affinity kinetics parameter ks M 1s 1 kg s1 Ka Ko Ka ka Ka ka ka Kinetics parameter ke kd 2 1 2 BIAevaluation Biqcore
10. a 7 pg 7 nn ns pg 160 Pri 150 2 SH 300 350 II 5 5HI 55II BDD ES 7DD Tirme BlAevaluation Software Handbook 24 hr rt rt tt 2 Kinetics Response Next gt BIAevaluation Software Handbook 25 2 Kinetics t Kinetics Simultanecus kldal 1 1 Langmuir binding Show hlndal Farameters hrega hlr 11 FC 1 lr Display cnnstants ornly lt Hack Fit Lance 1TTILan
11. Kinetics Ft Kinetics Separate ka Mipder 1 Langmuirl assnciatinn hnbs 1 Show Model FararTie ters Display constants only Cancel lkobs 1is Req RU RI RU Concofanalyte Chi2 ES 1 a 00416 150nE 0 029 8H 1nHn HI228 B 43 75n Hin1g1 10 1 5Hn 124 11 1 37 5n NN X Conc of analyte Y Values Y kobs 1 s OK reate Urwe ee lcanc of aaltg Canmce T alugs kabs BlAevaluation Software Handbook 41 2 Kinetics 5 5 IS5 1 1es 7 1 2eE 7 1 1 BeE 7 1E 7 1 1 ET 1 Next gt knhs ls ws Ennr of ahalyte BlAevaluation Software Handbook 42 2 Kinetics Linear fit Ft 5
12. 1e 3 4 1 BB 1g 3 1 1 3 Next gt lt Fit General PRE Steady state qffinity BIAevaluation Software Handbook 4 3 Affinity i HRT i lv Display cnnstanls only lt Back Fi Cancel EAN 2 9 KA1M KD M Rmax n Ch2 435e3 SS 32 4 2 3e 4 198 KA Kp BIAevaluation Software Handbook 48 4 4 Biacore
13. Name MAME TL Kinetics Fc 4 1 kinetics ble Kinetics Fc 4 2 kinetics ble Kinetics Fc 4 3 kinetics ble Kinetics Fc 4 4 kinetics ble kinetics ble kinetics F BIAevaluation Software Handbook 16 1 Vc we Curve Properties ame Proteins 5Hudml mm sm we mm Name OK 1HHI HH Frntein Sug EI Kinetics Fc 4 2 Kinetics Fc 4 3 Kinetics Fc 4 4 400 tn E Kinetics Fc 4 200 ab00 BIAevaluation Software Handbook 17 1 1 5 3 Curve Properties Color ght ed v oK Cu Properties 1 Iml lroteina SHu MTI CUFWE Fi LE CarnrEl ine sg Help
14. a O O hm
15. F OD HH 60 R 4 2
16. Kinetics 2 2 2 2 1 Global fitting 1 C Program Files BIAeval Amz2 bB Name Source apreeablr F 1 przezble Barr a hlr FC 1 1 Bre a hlr dd FC 1 pre2_ble apr2ezablr 10 FC 1 Pre2ble anreea hlr 11 FC 1 pre2ble BT a hlr 3 pree2_ble BT a hlr 33 nrEg_ hlg Br a hlr 34 FC 2 prezble Pr2zaablr 35 Pre2_ble a tl nl 5 32DDD 270DD 220DD RespOonSse TTD 12000 3 EO 1 132 n 1 TE Tims 400 100 1 5 hh 25 300 350 HH 5 SO SS 6DbD SH Tinmie BlAevaluation Software Handbook 23 2 Kinetics Ft Kinetics Simultaneous k k t Kinetics Simultaneous Next gt
17. Next gt Fit Kinatic Separate k IL namur dissociation FE OE EO SN im _ 281 3 53e 3 3 3 3 19e 3 SN BIAevaluation Software Handbook 36 2 Kinetics ky j Fit gt Kinetics Separate k k ANN RU El Association RU 160 14D0 120 100 B0 Il Response 4 2 20 150 ao0 Next gt Fit Kinetics Separate 1 1 LanEITUiF association Cemc 0 kd lap 22a blrB FC 1 ahlr11 FC 1 apbr22a blr 11 FC k BlAevaluation Software Handbook 37 2 Kinetics Fit Kinetics Sanarate akKd hipdel 1 1 Lamgmuir1 association Show Model Farameters 3
18. RU bound 9 A PROG Time window AbsResp SD Slope LRSD Quality Baseline RelResp Id SSaY 340 8 assay 339 8 assay 338 8 assay 338 8 1279 2 10 65 0 2 OK No 1002 6 bound 13UH 2 10 88 0 48 Ok No 1026 2 baund 852 8 4 11 0 75 Ok No 577 7 bound 556 9 2 11 1 21 Ok No 280 8 bound 401 7 2 18 1 38 OK No 125 1 bound 332 1 11 1 24 Ok No 54 6 bound 300 6 1 45 1 42 0k No 22 4 bound 285 8 1 62 1 49 OK No 7 bound assay 339 8 1326 2 11 14 5 0 95 Ok No 1047 9 bound assay 339 8 870 3 DB 0 81 Ok No 592 bound 5 5 5 5 assay 338 8 5 5 5 5 SSaY 339 8 5 5B1 1 58 1 25 Ok No 262 6 bound 5 5 5 5 5 5 5 5 5 5 assay 339 8 assay 339 8 SSaY 9 assay 339 8 405 1 72 1 48 Ok No 126 1 bound SSaY 339 8 db 1527 1 29 Ok No 54 bound SSaY 339 8 299 8 1 6 1 79 0k No 20 3 bnund assay 339 8 2866 4 1 63 1 53 Ok No 6 7 bound assay 339 8 927 5 DB 0 54 Ok Na 647 9 hnund assay 340 8 898 5 4 2 0 53 Ok No pb19 baund assay 340 8 682 9 2 0 97 Ok No 403 6 bound assay 339 8 924 2 4 21 0 75 Ok No 644 5 hnund assay 340 8 905 5 28 0 56 Ok Na 626 3 bound assay 339 8 BBp 1 0 81 Ok No 405 9 bound 4 2 3 Data Generate Calibration aselime RelResh Tat TI Keyword ng ml Y Keyword
19. 4 1 KEYWORD KEYWORD ng ml conc ng ml Excel ug ml nM uM unknown1 sqmple1 3 uk1 2 3 2 BIAevaluation Software Handbook 49 4 DEFINE APROG conc PARAM pos conc KEYWORD ng ml 9 conc
20. Y 10 20 5 248 4 KA Calculate Y Transform Transtorm OQ HLTgaT glInT 1 Kinetics Fc 4 1 Zero at Average of Selection Replace Original 8 6 BIAevaluation Software Handbook 1 U 1200 1000 1 4 3 x RU 350 300 2o50 200 3 150 100 50 D 50 150 1b0 50 D 50 100 150 ab0 2o50 Tirme BIAevaluation Software Handbook 1 Calculate gt X Transform Transtorm Ci i
21. Start BIAprogram BIAevaluation Ewaluation 1 2 C Program Files BIAeval KINETICS BLR ompetitiwe LaMSMUIR ass TransFer Swskem pen D Mu Documehnts rgg be CMC RPT TeT kinetics ble 1 BLE 5nlutinn Finity ble L Mn Lnmnuter 1 KINETICS BLR Mn Mahwnrk F Files of uhg al File Canmeel Open BIAevaluation Software Handbook 1 1 Biacore Kinetics blr BIAevaluation kinetics ble His tx Jpn inetic Elr Sensorgram Fc 4 TTiT 2 Sensorgram Fc 4 SeEnsorgram Fc 4 4 Sensorgram Fc 4 1 Sensorgram Fc 4 UE Tl Use Original Colors Use Original Colors B
22. Kinetics t Kinetics imut nnu karkd Next gt 9 Next gt BIAevaluation Software Handbook 29 2 Kinetics Fit Kinetics imultaneous ka kd lgdg 1 1 Langmuirl Binding how hiodel gpr22g bl 1U FC 1 PT 2gg hIF 11 F 1 ly Displaw cnnstants nnly ah RN eG Fit Fit 1 Response 150 2b00 250 JHH 350 4DD 450 5DD 550 EOD 650 HH Time S ka1 kd1 ka2 1 RUs kd2 1 s Rmax RU RI RU Conc of analyte Chi2 09e 3 352 SS 2 2 3 08e 4 4 S RCR blr 7 FC 1 3 150n apr22a blr 8 FC 1 Es 1 100n 75n ES br 10 FC 1 SUn ar blr 11 FC 1 375nS Parameter
23. RelResp OK BIAevaluation Software Handbook 4 ai Curye 4 2 4 yrle Unknnwn HelResn Calc nml cy nrmml 1 uk1 647 9 1111 032 FA uk1 bd 1104 956 i 107 394 0 39 18 UL 9 1039 7 1 uk 1072 Ot AVG zz D3 1066 158 0 8b 19 uk3 3 th 179 PP uk3 405 9 39 9 AVG 4 44 7 69 049 3H Calc ng ml AVG 2 CV BIAevaluation Software Handbook 5 5 BIAevaluation File Import Models jp BIA Ewaluation Prn ct ImEnt nade PrIrir BIAevaluation Software Handbook 4 5 C Program files BIAeval More Models md Import kiodels Lnnk Ii Bleeya pplata n rhE ItiyE LAMGPMLIR Mass Transher wstem To State Fores Models md esktn p Mn Docu
24. TE EE EE LITE jn EFFECT LT Et Et TRI EET Align To h BIAevaluation Software Handbook 1 lign To In Finish nl kl al il Al 1 Tam 1 ml 1bD0 150 250 Time BIAevaluation Software Handbook 1 1 4 4 C Program files Biaeval Hs des 1 2 3 14 1 13 1
25. TH 0 035 0 03 0 025 0 02 0 015 DJ ab de H EE H E Te r 1 26 7 1 4 7 1 hg Slope BE ET ISSNN Slop ks Intercept ko BIAevaluation Software Handbook 43 3 Affinity 3 Affinity Reqj ka 1x10 gt M 1S ka 1x10 gt M 1S ky 1x10 3 s 1 kg 1x10 1 s 1 kinetics parameter C Req KA Kp Req Ka C Rmax 1 KaC RU Req ver C BIAevaluation Software Handbook 44 3 Affinity
26. 1 1 Lqngmuir binding A B AB 1 1 1 1 1 1 binding with drifting baseline A B AB 1 1 1 1 binding with mass transfer A B AB 1 1 Bivalent analyte A B AB AB B AB2 2 2 AB AB 2 Heterogeneous analyte Competition reactions Al B A1B A2 B A2B 1 2 Heterogeneous ligand Parallel reaction A Bl ABl A B2 AB2 2 Two state reaqction conformation change A B gt AB ABX AB AB BlAevaluation Software Handbook 28 2
27. CAPTION 9 conc ng ml FLOW 5 INJECT pos 15 Isample 0 10 RPOINT baseline b 3 10 RPOINT Dound INJECT R2F3 5 Iregeneration solution 1 30 RPOINT regeneration END MAIN DETECTION 2 1 APROG conc ReA1 10 000 APROG conc _ RZ2AZ 5 000 APROG conc _ RZA3 2 500 APROG conc _ R2A4 1 250 APROG conc R2A5 625 APROG conc _ R2A1 10 000 APROG conc _ RZ2AZ 5 000 APROG conc _ RZA3 2 500 APROG conc _ R2A4 1 250 APROG conc R2A5 625 APROG conc _ R2B1 uk1 APROG conc _ R2B2 _ uk2 APROG conc _ R2B1 uk3 APROG conc _ R2B2 ukl APROG conc _ R2B1 uk2 APROG conc _ R2B2 _ uk3 APPENDStqndby END BlAevaluation Software Handbook 0 4 2 4 2 1 4 1 BIAeqvaluation File Open OK en 1 lr Sensorgram Fc Sensorgram Fc 2 Sensorgram Fc 3 Sensorgrarm Fc 4 Sensoargram Fc Sensorgram Fc 2 Sensorgram Fc 3 Sensorgrarm Fc 4 MM EE cle aAPROG 0 0 Re 5 0 AS ie 1assay 16176 7 0 0 09 Ok Yes 0 2 asSSaY 18234 4 0 05 0 15 Ok Yes 0 baseline 3 aSSaY 15100 4 0 02 0 17 Ok Yes 0 baseline 4 asSaY 16377 0 19 Ok Yes 0 baseline SSaY 2057 5 i 0 17 Ok Yes 0 baseline SSa
28. 0 WWW delifesclenceS COJD e mail Web 2009 GE GE 169 0073 3 25 1 TEL 03 5331 9336 FAX 03 5331 9370 e mail Tech JP ge com ISO 9001 2000
29. 02 3 73e 3 302 3 5e 3 302 3 45e 3 302 3 5e 3 302 3 3pe 3 Ww Display constants only Ft _ ka 1 Ms kd 1 s Rmax RUU RIIRU Conc of analyte KA 1 M KD MM Req RU kobs 1 s Chi2 2 52e5 3 73e 3 156 47 150n 56 77er 1 4Ba B 142 D41B 2 53e5 3Em 3 154 6 86 100n 7 D4e7 1 42e 8 135 00289 259e5 3 46e 3 154 B B z5n 7 5e7 1 33e B 131 0229 254e5 3 5e 3 153 3 5Hn Tber 1 3Be 8 120 HH1B2 aEI22ablr11 F6 1 2 4285 33Ee 3 151 1n 4 37 5n 719e7 1 39a 8 110 nn12 Residuals Report Farameters Resicduals Selections X 0 3 REsiHu BIAevaluation Software Handbook 38 2 Kinetics 2 3 ko k RT 3 Ks kass TKdjas Run different concentrations Plot dierivative vs R response dR dt vs R Plot slope vs C concentration K vs C 3
30. GE Healthcare BIQCOre BIAevaqluation Instrurment Handbook GE Imagination at work 71 3206 31 5z 1 1 RAO ON A 1 1 02 7 3 1 2 5 5 42 AAA 6 1 4 3 gt X O lt dd ii 124 4 NO 2 0 RSWRWOSWOSTS ST oo 10 13 CO A 15 15 16 1 5 3 18 MO Li 19 2 Kinetics 20 7 20 2 1 1 20 2 1 2 BIAevagluation 20 SS SO 22 BPR 23 2 2 1 Global fitting 23 2 2 2 Local fitting eeeseeeeesessseesesssessssssssssssssssss 31 2 33 2 35 2 39 3 Affinity ee 44 49 49 1 51 OI DO MA 51 52 52 AU 53 5 8M 54 1 1 1 1
31. LS 33 100n 6 1e7 1 64e 8 aprz2a blr 9 FC 1 2 31e5 371e 3 TS2 12 9 Tn 5 23er 1 6e 8 apr22a blr 10 FC 1 2 25e5 3 73e 3 156 13 2 5On 6 04e7 1 66e 8 apr22a blr 11 FC 1 2 16e5 3 54e 3 156 12 9 3T oh 6 1er 1 64e 8 Rmax K kg BIAevaluation Software Handbook 32 135 130 12S 109 U 0383 0 0269 0 0211 0 015 0 011 2 Kinetics 2 2 3 Global fitting 1 kinetics Ks kG RM 0 Rmax Rmax Rmax
32. Me5 1 3 8 144 1Un 1 8 A E 1 3 zn 7 1 1 8 34 5 1 3 AM un 7 1 BL 1 3 TN 8 WI 1 8 Residuals Selections Rmax A Rmax k Global fitting BlAevaluation Software Handbook 34 2 Kinetics 2 4 C Program Files BlAeval An22 2 wR RF RU 16D0 140 120 100 1 5 2D0D 25 3 35 HI SO EO 5 Tirmnme it Kinetics Simultanecus Next gt Fit Kinetics eparate Ea rE BlAevaluation Software Handbook 35 2 Kinetics Dissociation jp
33. Y 276 6 0 21 Ok Yes 0 baseline 1 assaY 15174 1 0 11 Ok No 2 5 bound 2 assay 18236 1 0 96 Ok 1 7 bound 3 aSSaY 16112 7 12 3 bound 4 assay 17409 7 1032 7 bound assay 2061 1 3 5 bound SSaY 1279 2 10 55 1002 6 bound 1 assay 1B171 8 0 19 4 9 regeneration 2 assay 18223 8 0 21 10 6 reqeneratian 3lassay 16095 2 0 18 5 2 regeneration 4 asSaY 16367 7 0 21 9 2 regeneration assay 2052 0 26 5 6 regeneration assay 272 6 0 27 4 regeneration 1 assay 15174 4 0 1 0 baseline 2 aSSaY 18228 4 0 19 0 baseline 3 assay 16098 6 0 09 0 baseline 4 assay 15372 6 0 19 0 baseline assay 2054 0 15 0 baseline assay 274 0 2 0 baseline 1 assay 1B1B3 4 45 10 9 bound 2 assay 18230 6 3 81 1 2 2 bound 3 aSSaY 16109 8 11 38 11 2 bound 4 assay 17430 9 20 78 1058 3 bound SSaY 2064 7 10 6 bound assay 13UH 2 10 88 5 102B 2 baund d 1 assay 16169 7 0 18 4 7 reqeneratinn o tu LU ho tu LU RNNIIUIN MDINRGNIIDSH 5 LUD LO LU mmnmmmmnmmnmmnmmnmnmmnmmnmnmmnommmnomnmnomn mn on mn mn on BIAevaluation Software Handbook 1 4 4 2 2 Data Filter Fc Ild
34. amu binding 28 Conc M aM fM pM nM uM mM M kM Fit BIAevaluation Software Handbook 26 2 Kinetics 160 EspOnsE 151 ab 3HII 35 II 5 5 55II Eh E5I bn Tirme SS 3 Hde J 153 SS bzHer 1 5de HN RR 3 NN sn ka Kg RI bulk effect KA Kp Redq 5 CA VE 3 5 6 BIAevaluation Software Handbook 27 2 Kinetics At B
35. colaur Light Red Marker 1Hmm RU Frateih interaction 12D00 1DD0 nn Frntein 5LIudJTTI Kinetics Fc 4 2 9 El _ Rinetics Fec 4 3 Rinetics Fc 4 OU Ed kinetics Fc 4 5 200 n a00 n En 120 18n 240 300 360 420 ah 54D En Timie BIAevaluation Software Handbook 18 1 1 5 4 View Scqle Auto Y OK RU Frateih A interaction TDD HI HI Th Fratein Sbugwnrl 600 kinetics Fc 4 2 HI kinetics Fc 4 3 HI kinetics Fc 4 4 DD Kinetics Fc 4 5 200 100 1DD SH TO 150 25 HI 350 HI 5 5HI 5 Time BlAevaluation Software Handbook 19 2 Kinetics 2 Kinetics 2 1 2 1 1 Affinity Affinity
36. ekics blr 5 kinetics Fc 4 5 Kinehirs_ hlr BIAevaluation Software Handbook 1 5 2 1 5 1 Re 24S00 5 anoo 5 22000 1 5 Time J CUTVG Kinehicg Fc 4 1 nc BlAevaluation Software Handbook 1 1 4 1 4 1 Time Edit Cut X 4 Response 24200 238 BIAevaluation Software Handbook 1 1 4 2
37. iacore Biacore3000 2000 1000 Biacore X Use Original Colors OK BIAevaluation Software Handbook 1 1 3 OK Einetics Fc 4 Einektics blr kinetics Fc 4 Einetics blr Einekics Fc 4 Kinekics blr Einetics Fc 4 Einetics blr Einetics Fc 4 Kinehrs hlr Ctrl Shift Name Source Einetics Fc 4 Einetics blr 1 1 Kinetics Fc 4 Einetics blr kinetics Fc 4 3 Kinehrs hlr Einekics Fc 4 4 Kin
38. ied X amp yY OK 1BHHI 12000 1IHHn HH BI III ab0b0 ab00 e se 300 HI 1300 183b00 BIAevaluation Software Handbook 11 1 File Close Window Project _Source his ttt1 HIS TXT hig t 1 Clpboard Paste 1 nisse1 ms TxT 2 3 Il Hame SOuUrce Clipboard Paste CIn hnard Faste CHI hnard Faste 3 5 350 ao50 150 BIAevaluation Software Handbook 12 1 1 4 5
39. ments File hame More odels md Files of tnpe Madal Files mdl Lance 2 Mu Nahwark PF Open Import kiodels Ipmrt Langmulr assnclalinr kobs IhHinn wlh Eponentlal baselne dr 1 binding with mass transfer kmlms IRM Help Imnnrl dd gt Emawe ES Add gt Import 96 BIAevaluation Software Handbook H9 3 NN OU 6 DR 9
40. riginal Add As New NN 6 PU gi a 1 150 hn 250 Time 8 BIAevaluation Software Handbook 14 1 1 5 1 5 1 View Labels OkK Y Labels Auto Labels RU Fratein A Ihteractinn 120D0 T1000 HI EHII R EHP E 400 200 ab0 BIAevaluation Software Handbook 15 1 1 5 2 View gt Legend OK Hidden Left Top TD Right Bottom lt 6D 120 18 240 S40 EOD
41. s Residuals Selections BIAevaluation Software Handbook 30 2 Kinetics 2 2 2 Local fitting Fit Kinetics Simultaneous k k t Finetics Simultaneous arkd Next gt 9 Next gt BIAevaluation Software Handbook 31 2 Kinetics Fit Kinetics imultaneous ka kd Show ng lgdg 1 1 Langmuirl binding araImBtBT Conc ai22g5lf 6 50n ly Displaw cnnstants nnly a heel Display constants only 1 apbF22ablrr 1 Rmax ko ka Fit Global Fit Local Ft ka 17Ms kd 17s Rmax RU RI RU Conc of analyte KA 1 M KD M Req RU kobs 1 s pr 22g blr FC 1 2 29e5 3 92e 3 150 12 3 150n 5 84e7 1 71e 8 apr22a blr 8 FC 1 2 3165 3 78e 3
42. tting k Curve fitting ke 12b00 TO GIIH EO 400 200 D BIAevaluation BIAevaluation Software Handbook 21 2 Kinetics Global fitting Local fitting 2 Local fitting kinetics ko ku 5 5 Global fitting Curve fitting kinetics Global fitting
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